immunocap  (Thermo Fisher)


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    Name:
    ImmunoCAP IgA IgG Calibrator ImmunoCAP
    Description:

    Catalog Number:
    14-4424-01
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    Structured Review

    Thermo Fisher immunocap
    Correlation between <t>ImmunoCAP</t> assay and DELFIA (Spearman's rank test, P

    https://www.bioz.com/result/immunocap/product/Thermo Fisher
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    immunocap - by Bioz Stars, 2020-07
    95/100 stars

    Images

    1) Product Images from "New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis"

    Article Title: New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis

    Journal: Clinical and Vaccine Immunology : CVI

    doi: 10.1128/CVI.00498-15

    Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P
    Figure Legend Snippet: Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P

    Techniques Used: Immunocap ISAC Assay

    2) Product Images from "Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects"

    Article Title: Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects

    Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    doi: 10.1111/pai.12310

    No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via ImmunoCAP. Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p
    Figure Legend Snippet: No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via ImmunoCAP. Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p

    Techniques Used: Microarray

    Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p
    Figure Legend Snippet: Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Techniques Used: Microarray

    3) Product Images from "Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components"

    Article Title: Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0231344

    Positive CCD-sIgE patients resulting in false-positive results were tested against ImmunoCAP streptavidin test (O212) with no allergenic proteins present. A single patient was not included due to insufficient volume.
    Figure Legend Snippet: Positive CCD-sIgE patients resulting in false-positive results were tested against ImmunoCAP streptavidin test (O212) with no allergenic proteins present. A single patient was not included due to insufficient volume.

    Techniques Used:

    Change in specific IgE results against recombinant components before and after CCD inhibition focusing on the 14 false-positive results observed on ImmunoCAP. (a) NOVEOS Specific IgE results. (b) ImmunoCAP Specific IgE results.
    Figure Legend Snippet: Change in specific IgE results against recombinant components before and after CCD inhibition focusing on the 14 false-positive results observed on ImmunoCAP. (a) NOVEOS Specific IgE results. (b) ImmunoCAP Specific IgE results.

    Techniques Used: Recombinant, Inhibition

    4) Product Images from "Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study"

    Article Title: Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2015.09.052

    A and B , Likelihood ( y-axis , percentage) of reporting symptoms to cat at 16 years of age depending of the number of IgE-reactive cat allergens (Fel d 1, Fel d 2, and Fel d 4) and ImmunoCAP cat extract sensitization (x-axes) at 4 (Fig 5, A ) and 8 (Fig 5, B ) years of age. C and D , Likelihood ( y-axis , percentage) of reporting symptoms to dog at 16 years of age depending of the number of IgE-reactive dog allergens (Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6) and ImmunoCAP dog extract sensitization (x-axes) at 4 (Fig 5, C ) and 8 (Fig 5, D ) years of age. Numbers, ORs, and 95% CIs are shown (n = 779).
    Figure Legend Snippet: A and B , Likelihood ( y-axis , percentage) of reporting symptoms to cat at 16 years of age depending of the number of IgE-reactive cat allergens (Fel d 1, Fel d 2, and Fel d 4) and ImmunoCAP cat extract sensitization (x-axes) at 4 (Fig 5, A ) and 8 (Fig 5, B ) years of age. C and D , Likelihood ( y-axis , percentage) of reporting symptoms to dog at 16 years of age depending of the number of IgE-reactive dog allergens (Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6) and ImmunoCAP dog extract sensitization (x-axes) at 4 (Fig 5, C ) and 8 (Fig 5, D ) years of age. Numbers, ORs, and 95% CIs are shown (n = 779).

    Techniques Used:

    5) Product Images from "Mechanisms, safety and efficacy of a B cell epitope-based vaccine for immunotherapy of grass pollen allergy"

    Article Title: Mechanisms, safety and efficacy of a B cell epitope-based vaccine for immunotherapy of grass pollen allergy

    Journal: EBioMedicine

    doi: 10.1016/j.ebiom.2016.08.022

    Quantification of grass pollen allergen-specific IgE levels in the treatment groups before and after vaccination. Timothy grass pollen allergen extract-specific IgE levels determined by ImmunoCAP (y-axes: kUA/l, horizontal bars denote medians) in sera from the four patient groups (S1: 10 μg; S2: 20 μg; S3: 40 μg; placebo) before (pre) and after (post) treatment.
    Figure Legend Snippet: Quantification of grass pollen allergen-specific IgE levels in the treatment groups before and after vaccination. Timothy grass pollen allergen extract-specific IgE levels determined by ImmunoCAP (y-axes: kUA/l, horizontal bars denote medians) in sera from the four patient groups (S1: 10 μg; S2: 20 μg; S3: 40 μg; placebo) before (pre) and after (post) treatment.

    Techniques Used:

    6) Product Images from "Comparison of an automated microfluidic immunoassay technology (BioIC, lab-on-chips) and ImmunoCAP assay. Lab-on-chips as a tool for specific IgE (sIgE) detection"

    Article Title: Comparison of an automated microfluidic immunoassay technology (BioIC, lab-on-chips) and ImmunoCAP assay. Lab-on-chips as a tool for specific IgE (sIgE) detection

    Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

    doi: 10.5114/ada.2020.93383

    ROC curves for ImmunoCAP ( A ) and BioIC ( B )
    Figure Legend Snippet: ROC curves for ImmunoCAP ( A ) and BioIC ( B )

    Techniques Used:

    7) Product Images from "Evaluation of a Novel Rapid Test System for the Detection of Specific IgE to Hymenoptera Venoms"

    Article Title: Evaluation of a Novel Rapid Test System for the Detection of Specific IgE to Hymenoptera Venoms

    Journal: Journal of Allergy

    doi: 10.1155/2012/862023

    Spearman correlation diagram of ALFA versus ImmunoCAP for the detection of sIgE to bee venom. Quantitative agreement was found with a Spearman correlation coefficient of 0.72 ( P
    Figure Legend Snippet: Spearman correlation diagram of ALFA versus ImmunoCAP for the detection of sIgE to bee venom. Quantitative agreement was found with a Spearman correlation coefficient of 0.72 ( P

    Techniques Used:

    Spearman correlation diagram of ALFA versus ImmunoCAP for the detection of sIgE to wasp venom. Quantitative agreement was found with a Spearman correlation coefficient of 0.82 ( P
    Figure Legend Snippet: Spearman correlation diagram of ALFA versus ImmunoCAP for the detection of sIgE to wasp venom. Quantitative agreement was found with a Spearman correlation coefficient of 0.82 ( P

    Techniques Used:

    Graphical illustration of the relation of total IgE (green diamond, determined by ImmunoCAP) and sIgE to BV (red) and WV (blue) for ImmunoCAP (a) and ALFA (b) for each study group.
    Figure Legend Snippet: Graphical illustration of the relation of total IgE (green diamond, determined by ImmunoCAP) and sIgE to BV (red) and WV (blue) for ImmunoCAP (a) and ALFA (b) for each study group.

    Techniques Used:

    Receiver operating characteristic of ALFA and ALLERGO-LIQ for the diagnosis of bee (a) and wasp (b) venom allergy in monosensitized patients (group A) and control groups (C and D). Curve with dots indicates results for ALFA and curve with squares for ALLERGO-LIQ. Diagnosis of insect venom allergy was based on patient's history, skin testing, and detection of sIgE to bee or wasp venom by ImmunoCAP. Sensitivity/specificity for ALFA is 100%/83% (BV) and 82%/97% (WV) at a cut-off value of 10.0 RU and 100%/93% (BV) and 82%/93% (WV) for ALLERG-O-LIQ.
    Figure Legend Snippet: Receiver operating characteristic of ALFA and ALLERGO-LIQ for the diagnosis of bee (a) and wasp (b) venom allergy in monosensitized patients (group A) and control groups (C and D). Curve with dots indicates results for ALFA and curve with squares for ALLERGO-LIQ. Diagnosis of insect venom allergy was based on patient's history, skin testing, and detection of sIgE to bee or wasp venom by ImmunoCAP. Sensitivity/specificity for ALFA is 100%/83% (BV) and 82%/97% (WV) at a cut-off value of 10.0 RU and 100%/93% (BV) and 82%/93% (WV) for ALLERG-O-LIQ.

    Techniques Used:

    8) Product Images from "Single Nanoparticle Detection for Multiplexed Protein Diagnostics with Attomolar Sensitivity in Serum and Unprocessed Whole Blood"

    Article Title: Single Nanoparticle Detection for Multiplexed Protein Diagnostics with Attomolar Sensitivity in Serum and Unprocessed Whole Blood

    Journal: Analytical chemistry

    doi: 10.1021/ac4000514

    Multiplexed assay to detect specific IgE to eight allergens. Fluorescence measurement was used as a control to validate nanoparticle counting detection ( R 2 = 0.97 for serum both and whole blood samples). Human sample was characterized with Phadia ImmunoCAP or skin prick testing (SPT): (+) reflects a positive result. Error bars correspond to the standard deviation from the mean ( n = 4). The * indicates that the results were not determined.
    Figure Legend Snippet: Multiplexed assay to detect specific IgE to eight allergens. Fluorescence measurement was used as a control to validate nanoparticle counting detection ( R 2 = 0.97 for serum both and whole blood samples). Human sample was characterized with Phadia ImmunoCAP or skin prick testing (SPT): (+) reflects a positive result. Error bars correspond to the standard deviation from the mean ( n = 4). The * indicates that the results were not determined.

    Techniques Used: Fluorescence, Single-particle Tracking, Standard Deviation

    9) Product Images from "Allergen Component Specific IgE Measurement With the Immulite™ 2000 System: Diagnostic Accuracy and Intermethod Comparison"

    Article Title: Allergen Component Specific IgE Measurement With the Immulite™ 2000 System: Diagnostic Accuracy and Intermethod Comparison

    Journal: Journal of Clinical Laboratory Analysis

    doi: 10.1002/jcla.21741

    Scatterplot of intermethod comparison between Immulite™ 2000 and ImmunoCAP™ for Ole e 1, Bet v1, Bet v 2, Pru p 3, Pen m 1/Pen a 1, individually and altogether considered. Dotted line: 95% confidence interval.
    Figure Legend Snippet: Scatterplot of intermethod comparison between Immulite™ 2000 and ImmunoCAP™ for Ole e 1, Bet v1, Bet v 2, Pru p 3, Pen m 1/Pen a 1, individually and altogether considered. Dotted line: 95% confidence interval.

    Techniques Used:

    10) Product Images from "The usefulness of casein-specific IgE and IgG4 antibodies in cow's milk allergic children"

    Article Title: The usefulness of casein-specific IgE and IgG4 antibodies in cow's milk allergic children

    Journal: Clinical and Molecular Allergy : CMA

    doi: 10.1186/1476-7961-10-1

    ROC curves showing the performance of the ImmunoCAP tests for milk-, casein-, α-lactalbumin- and β-lactoglobulin-specific IgE antibodies in relation to the diagnosis of cow's milk allergy .
    Figure Legend Snippet: ROC curves showing the performance of the ImmunoCAP tests for milk-, casein-, α-lactalbumin- and β-lactoglobulin-specific IgE antibodies in relation to the diagnosis of cow's milk allergy .

    Techniques Used:

    11) Product Images from "Detection of Allergen Specific Antibodies From Nasal Secretion of Allergic Rhinitis Patients"

    Article Title: Detection of Allergen Specific Antibodies From Nasal Secretion of Allergic Rhinitis Patients

    Journal: Allergy, Asthma & Immunology Research

    doi: 10.4168/aair.2016.8.4.329

    Detection of local specific antibodies in the nasal secretion of the NPT-positive and NPT-negative groups. (A) Total IgE, (B) specific IgE to D. farinae were detected by using the ImmunoCAP, (C) specific IgE to D. farinae , (D) specific IgA to D. farinae , and (E) secretory IgA to D. farinae were measured by using ELISA. The horizontal bars indicate positive cutoff values (mean+3 SD). All values were adjusted for the protein ratio. NPT, nasal provocation test.
    Figure Legend Snippet: Detection of local specific antibodies in the nasal secretion of the NPT-positive and NPT-negative groups. (A) Total IgE, (B) specific IgE to D. farinae were detected by using the ImmunoCAP, (C) specific IgE to D. farinae , (D) specific IgA to D. farinae , and (E) secretory IgA to D. farinae were measured by using ELISA. The horizontal bars indicate positive cutoff values (mean+3 SD). All values were adjusted for the protein ratio. NPT, nasal provocation test.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    12) Product Images from "Cross-reactive LTP sensitization in food-dependent exercise-induced urticaria/anaphylaxis: a pilot study of a component-resolved and in vitro depletion approach"

    Article Title: Cross-reactive LTP sensitization in food-dependent exercise-induced urticaria/anaphylaxis: a pilot study of a component-resolved and in vitro depletion approach

    Journal: Clinical and Translational Allergy

    doi: 10.1186/s13601-016-0136-5

    ISAC microarray results comparing native and depleted serum for each patient. The suspected culprit allergens in each case are shown in white boxes . Serum was pre-incubated with ImmunoCAP peach (f 95) in patient I, walnut (f 256) in patient II, and wheat (f 4) in patient III
    Figure Legend Snippet: ISAC microarray results comparing native and depleted serum for each patient. The suspected culprit allergens in each case are shown in white boxes . Serum was pre-incubated with ImmunoCAP peach (f 95) in patient I, walnut (f 256) in patient II, and wheat (f 4) in patient III

    Techniques Used: Microarray, Incubation

    13) Product Images from "Novel birch pollen specific immunotherapy formulation based on contiguous overlapping peptides"

    Article Title: Novel birch pollen specific immunotherapy formulation based on contiguous overlapping peptides

    Journal: Clinical and Translational Allergy

    doi: 10.1186/2045-7022-3-17

    Human basophil degranulation assay. Panel A : Basophils from two subjects allergic to birch (circle and square symbols) were activated by rBet v 1, AllerT, T4-T5 or T6-T7-T8 mixes at indicated concentrations. Panel B : Basophils from a non-allergic donor were pre-incubated with sera from 3 subjects allergic to birch (circles, squares and triangles) and incubated with the indicated concentrations of rBet v 1 and AllerT. Degranulation is expressed as the percentage of CD63 positive basophils. Panel C : Rat basophils expressing hu FcϵRI were pre-incubated with sera from 10 subjects allergic birch and reacted with either rBet v 1 or AllerT (filled and open symbols respectively). Results were grouped by IgE class levels as determined by the ImmunoCAP assay (classes 4 to 6; n= 3 or 4 sera per class). Hexosaminidase release is expressed as the percentage of release induced by anti-total IgE antiserum.
    Figure Legend Snippet: Human basophil degranulation assay. Panel A : Basophils from two subjects allergic to birch (circle and square symbols) were activated by rBet v 1, AllerT, T4-T5 or T6-T7-T8 mixes at indicated concentrations. Panel B : Basophils from a non-allergic donor were pre-incubated with sera from 3 subjects allergic to birch (circles, squares and triangles) and incubated with the indicated concentrations of rBet v 1 and AllerT. Degranulation is expressed as the percentage of CD63 positive basophils. Panel C : Rat basophils expressing hu FcϵRI were pre-incubated with sera from 10 subjects allergic birch and reacted with either rBet v 1 or AllerT (filled and open symbols respectively). Results were grouped by IgE class levels as determined by the ImmunoCAP assay (classes 4 to 6; n= 3 or 4 sera per class). Hexosaminidase release is expressed as the percentage of release induced by anti-total IgE antiserum.

    Techniques Used: Degranulation Assay, Incubation, Expressing, Immunocap ISAC Assay

    14) Product Images from "Comparison of an automated microfluidic immunoassay technology (BioIC, lab-on-chips) and ImmunoCAP assay. Lab-on-chips as a tool for specific IgE (sIgE) detection"

    Article Title: Comparison of an automated microfluidic immunoassay technology (BioIC, lab-on-chips) and ImmunoCAP assay. Lab-on-chips as a tool for specific IgE (sIgE) detection

    Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

    doi: 10.5114/ada.2020.93383

    ROC curves for ImmunoCAP ( A ) and BioIC ( B )
    Figure Legend Snippet: ROC curves for ImmunoCAP ( A ) and BioIC ( B )

    Techniques Used:

    15) Product Images from "Monitoring Allergen Immunotherapy Effects by Microarray"

    Article Title: Monitoring Allergen Immunotherapy Effects by Microarray

    Journal: Current Treatment Options in Allergy

    doi: 10.1007/s40521-016-0084-2

    Example of the calculation of the ratio of allergen-specific IgE and allergen molecules in a test system using allergen excess (e.g., ImmunoCAP).
    Figure Legend Snippet: Example of the calculation of the ratio of allergen-specific IgE and allergen molecules in a test system using allergen excess (e.g., ImmunoCAP).

    Techniques Used:

    Samples that are devoid of blocking IgG antibodies ( blue ) yield similar results for detection of IgE ( red ) in serological testing by microarray ( a ) and by tests that employ a large amount of allergen ( yellow ) per assay, like ImmunoCAP ( c ). By contrast, in presence of blocking IgG, competition between IgE and blocking IgG antibodies for binding to the same allergen molecule reduces IgE levels detected by microarray containing low amounts of allergen ( b ) but not in tests using large quantities of allergen ( d ).
    Figure Legend Snippet: Samples that are devoid of blocking IgG antibodies ( blue ) yield similar results for detection of IgE ( red ) in serological testing by microarray ( a ) and by tests that employ a large amount of allergen ( yellow ) per assay, like ImmunoCAP ( c ). By contrast, in presence of blocking IgG, competition between IgE and blocking IgG antibodies for binding to the same allergen molecule reduces IgE levels detected by microarray containing low amounts of allergen ( b ) but not in tests using large quantities of allergen ( d ).

    Techniques Used: Blocking Assay, Microarray, Binding Assay

    16) Product Images from "Reduction in allergen-specific IgE binding as measured by microarray: A possible surrogate marker for effects of specific immunotherapy"

    Article Title: Reduction in allergen-specific IgE binding as measured by microarray: A possible surrogate marker for effects of specific immunotherapy

    Journal: The Journal of Allergy and Clinical Immunology

    doi: 10.1016/j.jaci.2015.02.034

    Courses of geometric means and 95% CIs of Bet v 1–specific IgE ( A : ImmunoCAP; B : ISAC) and IgG ( C : ImmunoCAP; D : ISAC) levels (y-axes) over the study period (x-axes) for placebo- (blue) , fragment- (green) , and trimer-treated patients (orange) . * P
    Figure Legend Snippet: Courses of geometric means and 95% CIs of Bet v 1–specific IgE ( A : ImmunoCAP; B : ISAC) and IgG ( C : ImmunoCAP; D : ISAC) levels (y-axes) over the study period (x-axes) for placebo- (blue) , fragment- (green) , and trimer-treated patients (orange) . * P

    Techniques Used:

    Association of alterations in Bet v 1–specific IgE levels determined by ImmunoCAP and nasal allergen tolerance. Y-axes (positive range) indicate increased allergen-specific tolerance, wherein 1-point increase indicates tolerance of a 10-fold higher allergen concentration and 1-point decrease indicates a 10-fold higher sensitivity. X-axes indicate the log fold changes of Bet v 1–specific IgE when comparing prestudy levels (ie, before treatment; see Fig 1 and Fig E1) to levels measured after the pollen season (ie, after season; see Fig 1 and Fig E1).
    Figure Legend Snippet: Association of alterations in Bet v 1–specific IgE levels determined by ImmunoCAP and nasal allergen tolerance. Y-axes (positive range) indicate increased allergen-specific tolerance, wherein 1-point increase indicates tolerance of a 10-fold higher allergen concentration and 1-point decrease indicates a 10-fold higher sensitivity. X-axes indicate the log fold changes of Bet v 1–specific IgE when comparing prestudy levels (ie, before treatment; see Fig 1 and Fig E1) to levels measured after the pollen season (ie, after season; see Fig 1 and Fig E1).

    Techniques Used: Concentration Assay

    17) Product Images from "Measurement of Hymenoptera venom specific IgE by the IMMULITE 3gAllergy in subjects with negative or positive results by ImmunoCAP"

    Article Title: Measurement of Hymenoptera venom specific IgE by the IMMULITE 3gAllergy in subjects with negative or positive results by ImmunoCAP

    Journal: Asia Pacific Allergy

    doi: 10.5415/apallergy.2012.2.3.195

    Quantitative results for Hymenoptera sIgE according to IMMULITE 3gAllergy (IMMULITE) using its 0.1 IU A /mL cutoff among the 50 participants who had a history of Hymenoptera stings but who tested negative according to ImmunoCAP (CAP) (
    Figure Legend Snippet: Quantitative results for Hymenoptera sIgE according to IMMULITE 3gAllergy (IMMULITE) using its 0.1 IU A /mL cutoff among the 50 participants who had a history of Hymenoptera stings but who tested negative according to ImmunoCAP (CAP) (

    Techniques Used:

    Study design. SR, systemic reactions; CAP; ImmunoCAP.
    Figure Legend Snippet: Study design. SR, systemic reactions; CAP; ImmunoCAP.

    Techniques Used:

    Of the 21 participants with negative ImmunoCAP test results (
    Figure Legend Snippet: Of the 21 participants with negative ImmunoCAP test results (

    Techniques Used:

    18) Product Images from "Protein-bound polyphenols create “ghost” band artifacts during chemiluminescence-based antigen detection"

    Article Title: Protein-bound polyphenols create “ghost” band artifacts during chemiluminescence-based antigen detection

    Journal: F1000Research

    doi: 10.12688/f1000research.10622.2

    Protein distribution visualized by Coomassie Brilliant Blue staining (CBB), nitroblue tetrazolium (NBT) staining, and IgE binding capacity. ( A ) SDS-PAGE of unmodified egg white protein (CTL) or egg white protein-polyphenol aggregate particles containing 5, 10, 15, 30, and 40% polyphenols and stained with CBB; ( B ) Staining of green tea polyphenol-bound egg white proteins by NBT, following SDS-PAGE and subsequent electrophoretic transfer to a PVDF membrane; ( C ) corresponding Western blot. Pooled human plasma from 7 egg white-allergic individuals was used to bind antigens on the membrane. Egg white-specific IgE levels ranged from 15.4 to 100 kU L −1 as determined via ImmunoCAP (Phadia, Uppsula, Sweden). Biotinylated goat IgG anti-human IgE was used as the secondary antibody and NeutrAvidin HRP conjugate and substrate were used for signal production. M: molecular weight marker (kDa); CTL: control (unmodified egg white protein). Approximate locations for egg white allergens are indicated. Gray scale was used for gels and membranes and contrast was optimized to improve visualization.
    Figure Legend Snippet: Protein distribution visualized by Coomassie Brilliant Blue staining (CBB), nitroblue tetrazolium (NBT) staining, and IgE binding capacity. ( A ) SDS-PAGE of unmodified egg white protein (CTL) or egg white protein-polyphenol aggregate particles containing 5, 10, 15, 30, and 40% polyphenols and stained with CBB; ( B ) Staining of green tea polyphenol-bound egg white proteins by NBT, following SDS-PAGE and subsequent electrophoretic transfer to a PVDF membrane; ( C ) corresponding Western blot. Pooled human plasma from 7 egg white-allergic individuals was used to bind antigens on the membrane. Egg white-specific IgE levels ranged from 15.4 to 100 kU L −1 as determined via ImmunoCAP (Phadia, Uppsula, Sweden). Biotinylated goat IgG anti-human IgE was used as the secondary antibody and NeutrAvidin HRP conjugate and substrate were used for signal production. M: molecular weight marker (kDa); CTL: control (unmodified egg white protein). Approximate locations for egg white allergens are indicated. Gray scale was used for gels and membranes and contrast was optimized to improve visualization.

    Techniques Used: Staining, Binding Assay, SDS Page, CTL Assay, Western Blot, Molecular Weight, Marker

    19) Product Images from "Dramatically decreased T cell responses but persistent IgE upon reduced pollen exposure"

    Article Title: Dramatically decreased T cell responses but persistent IgE upon reduced pollen exposure

    Journal: Immunobiology

    doi: 10.1016/j.imbio.2019.07.003

    Exposure and prevalence of sensitization to mugwort pollen in Vienna from 1997-2018. (A.) Local total pollen counts for Artemisia vulgaris . The years when T cell experiments were performed and when sensitization of the population was assessed are indicated. (B.) Percentage of mugwort pollen-sensitized subjects as determined by skin prick test with pollen extract (1997–2007) and (C.) analysis of Art v 1-specific serum IgE-levels within all mugwort positive subjects (2009–2015) and (D.) within a single individual (2000–2007) (Pat7 is identical to Pat7 in Fig. 1C ). ImmunoCAP classes are defined as follows (kU/L):1, 0.35–0.70; 2, 0.7 0–3.5; 3, 3.5–17.5; 4, 17.5–50; 5, 50–100; 6, > 100.
    Figure Legend Snippet: Exposure and prevalence of sensitization to mugwort pollen in Vienna from 1997-2018. (A.) Local total pollen counts for Artemisia vulgaris . The years when T cell experiments were performed and when sensitization of the population was assessed are indicated. (B.) Percentage of mugwort pollen-sensitized subjects as determined by skin prick test with pollen extract (1997–2007) and (C.) analysis of Art v 1-specific serum IgE-levels within all mugwort positive subjects (2009–2015) and (D.) within a single individual (2000–2007) (Pat7 is identical to Pat7 in Fig. 1C ). ImmunoCAP classes are defined as follows (kU/L):1, 0.35–0.70; 2, 0.7 0–3.5; 3, 3.5–17.5; 4, 17.5–50; 5, 50–100; 6, > 100.

    Techniques Used:

    20) Product Images from "Allergen Chip Diagnosis for Soy-Allergic Patients: Gly m 4 as a Marker for Severe Food-Allergic Reactions to Soy"

    Article Title: Allergen Chip Diagnosis for Soy-Allergic Patients: Gly m 4 as a Marker for Severe Food-Allergic Reactions to Soy

    Journal: International archives of allergy and immunology

    doi: 10.1159/000345970

    Linear regression analysis of Gly m 4-specific IgE measured by Gly m 4 ImmunoCAP and ISAC in the soy-allergic patients (n = 20). Dotted lines indicate 0.1 kUA/l cutoff level for ImmunoCAP and 0.3 ISU for ImmunoCAP ISAC 112.
    Figure Legend Snippet: Linear regression analysis of Gly m 4-specific IgE measured by Gly m 4 ImmunoCAP and ISAC in the soy-allergic patients (n = 20). Dotted lines indicate 0.1 kUA/l cutoff level for ImmunoCAP and 0.3 ISU for ImmunoCAP ISAC 112.

    Techniques Used:

    21) Product Images from "Cross-Reactivity between Oak and Birch Pollens in Korean Tree Pollinosis"

    Article Title: Cross-Reactivity between Oak and Birch Pollens in Korean Tree Pollinosis

    Journal: Journal of Korean Medical Science

    doi: 10.3346/jkms.2016.31.8.1202

    Cross-reactivity by PR-10 allergens. Inhibition ImmunoCAP analysis against Bet v 1 was performed using sawtooth oak, Mongolian oak, white oak, and common silver birch pollen extracts.
    Figure Legend Snippet: Cross-reactivity by PR-10 allergens. Inhibition ImmunoCAP analysis against Bet v 1 was performed using sawtooth oak, Mongolian oak, white oak, and common silver birch pollen extracts.

    Techniques Used: Inhibition

    22) Product Images from "Anaphylactic reaction in patient allergic to mango"

    Article Title: Anaphylactic reaction in patient allergic to mango

    Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

    doi: 10.1186/s13223-018-0294-1

    Results of the inhibition with the mango allergen on the ImmunoCap matrix
    Figure Legend Snippet: Results of the inhibition with the mango allergen on the ImmunoCap matrix

    Techniques Used: Inhibition

    23) Product Images from "Sensitization to cereals and peanut evidenced by skin prick test and specific IgE in food-tolerant, grass pollen allergic patients"

    Article Title: Sensitization to cereals and peanut evidenced by skin prick test and specific IgE in food-tolerant, grass pollen allergic patients

    Journal: Clinical and Translational Allergy

    doi: 10.1186/2045-7022-1-15

    Quantitative relation of sIgE against the different cereals, peanut and bromelain . Immulite is plotted against ImmunoCAP for each cereal, peanut and bromelain on double-log-scale. Values depicted at 0.01 represent values
    Figure Legend Snippet: Quantitative relation of sIgE against the different cereals, peanut and bromelain . Immulite is plotted against ImmunoCAP for each cereal, peanut and bromelain on double-log-scale. Values depicted at 0.01 represent values

    Techniques Used:

    Positive results of SPT and sIgE . A . SPT mean wheal sizes of positive results. n = number of positives. Number of positive results > 7 mm 2 in parentheses. The inserted table shows percentages of positives with or without application of 7 mm 2 as cut off. B . Immulite, means of positive sIgE results > 0.35 kU A /L on log-scale. Percent positive in parentheses. C . ImmunoCAP, means of positive sIgE results > 0.35 kU A /L on log-scale. Percent positive in parentheses.
    Figure Legend Snippet: Positive results of SPT and sIgE . A . SPT mean wheal sizes of positive results. n = number of positives. Number of positive results > 7 mm 2 in parentheses. The inserted table shows percentages of positives with or without application of 7 mm 2 as cut off. B . Immulite, means of positive sIgE results > 0.35 kU A /L on log-scale. Percent positive in parentheses. C . ImmunoCAP, means of positive sIgE results > 0.35 kU A /L on log-scale. Percent positive in parentheses.

    Techniques Used: Single-particle Tracking

    Cluster diagrams and chi 2 -test results of relation between the cereals and peanut . A . Skin prick test. B . Immulite. C . ImmunoCAP. D . Number of patients reacting to peanut in the different clusters.
    Figure Legend Snippet: Cluster diagrams and chi 2 -test results of relation between the cereals and peanut . A . Skin prick test. B . Immulite. C . ImmunoCAP. D . Number of patients reacting to peanut in the different clusters.

    Techniques Used:

    Cluster diagrams showing the distribution of positives to bromelain . Positives to bromelain are shown in the white shaded circles. Colour codes are as in Figure 3. A . Immulite. B . ImmunoCAP.
    Figure Legend Snippet: Cluster diagrams showing the distribution of positives to bromelain . Positives to bromelain are shown in the white shaded circles. Colour codes are as in Figure 3. A . Immulite. B . ImmunoCAP.

    Techniques Used:

    24) Product Images from "Allergen sensitization in a birth cohort at mid-childhood: focus on food component IgE and IgG4 responses"

    Article Title: Allergen sensitization in a birth cohort at mid-childhood: focus on food component IgE and IgG4 responses

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2017.07.029

    A , IgE sensitization to inhalant and food allergen extracts in 6-10 year olds (n = 616) as measured by ImmunoCAP. B , Comparison of IgE results from microchip assay with results from the extract assay. C , Comparison of food-specific components with ImmunoCAP singleplex and ISAC. Class sensitization (on x-axis ) was determined by extract testing and statistics reflect the χ 2 test with Yates correction.
    Figure Legend Snippet: A , IgE sensitization to inhalant and food allergen extracts in 6-10 year olds (n = 616) as measured by ImmunoCAP. B , Comparison of IgE results from microchip assay with results from the extract assay. C , Comparison of food-specific components with ImmunoCAP singleplex and ISAC. Class sensitization (on x-axis ) was determined by extract testing and statistics reflect the χ 2 test with Yates correction.

    Techniques Used: MicroChIP Assay

    A , Representative results of samples that were tested for IgE and IgG4 to cow's milk and peanut components by ImmunoCAP, and for IgE to components by ISAC. B , Specific IgG4 levels from samples that were positive to the component-relevant food specific IgE by extract testing where threshold of detection of specific IgG4 was 0.07 μg/ml. Data represents geometric means where error bars are the 95% CI. Values less than the threshold of detection are shown below the dotted line and are not included in the geometric mean. C , Milk (n=20) and peanut (n=23)-specific components were plotted for IgE and IgG4. Where IgE and IgG4 were less than the detection limit they were depicted with the values 0.25 kU/L and 0.04 μg/mL, respectively.
    Figure Legend Snippet: A , Representative results of samples that were tested for IgE and IgG4 to cow's milk and peanut components by ImmunoCAP, and for IgE to components by ISAC. B , Specific IgG4 levels from samples that were positive to the component-relevant food specific IgE by extract testing where threshold of detection of specific IgG4 was 0.07 μg/ml. Data represents geometric means where error bars are the 95% CI. Values less than the threshold of detection are shown below the dotted line and are not included in the geometric mean. C , Milk (n=20) and peanut (n=23)-specific components were plotted for IgE and IgG4. Where IgE and IgG4 were less than the detection limit they were depicted with the values 0.25 kU/L and 0.04 μg/mL, respectively.

    Techniques Used:

    25) Product Images from "Detection of Allergen Specific Antibodies From Nasal Secretion of Allergic Rhinitis Patients"

    Article Title: Detection of Allergen Specific Antibodies From Nasal Secretion of Allergic Rhinitis Patients

    Journal: Allergy, Asthma & Immunology Research

    doi: 10.4168/aair.2016.8.4.329

    Detection of local specific antibodies in the nasal secretion of the NPT-positive and NPT-negative groups. (A) Total IgE, (B) specific IgE to D. farinae were detected by using the ImmunoCAP, (C) specific IgE to D. farinae , (D) specific IgA to D. farinae , and (E) secretory IgA to D. farinae were measured by using ELISA. The horizontal bars indicate positive cutoff values (mean+3 SD). All values were adjusted for the protein ratio. NPT, nasal provocation test.
    Figure Legend Snippet: Detection of local specific antibodies in the nasal secretion of the NPT-positive and NPT-negative groups. (A) Total IgE, (B) specific IgE to D. farinae were detected by using the ImmunoCAP, (C) specific IgE to D. farinae , (D) specific IgA to D. farinae , and (E) secretory IgA to D. farinae were measured by using ELISA. The horizontal bars indicate positive cutoff values (mean+3 SD). All values were adjusted for the protein ratio. NPT, nasal provocation test.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    26) Product Images from "Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure"

    Article Title: Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure

    Journal: Annals of Laboratory Medicine

    doi: 10.3343/alm.2018.38.4.338

    Correlation analysis of three in vitro diagnostic platforms: GOLD chip, ImmunoCAP, and AdvanSure. The sera of 150 patients with allergy were screened for seven of the most common allergens, and the amount of allergen-specific IgE (sIgE) measured by each platform was plotted for Pearson's correlation analysis.
    Figure Legend Snippet: Correlation analysis of three in vitro diagnostic platforms: GOLD chip, ImmunoCAP, and AdvanSure. The sera of 150 patients with allergy were screened for seven of the most common allergens, and the amount of allergen-specific IgE (sIgE) measured by each platform was plotted for Pearson's correlation analysis.

    Techniques Used: In Vitro, Diagnostic Assay, Chromatin Immunoprecipitation

    27) Product Images from "Anaphylactic reaction in patient allergic to mango"

    Article Title: Anaphylactic reaction in patient allergic to mango

    Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

    doi: 10.1186/s13223-018-0294-1

    Results of the inhibition with the mango allergen on the ImmunoCap matrix
    Figure Legend Snippet: Results of the inhibition with the mango allergen on the ImmunoCap matrix

    Techniques Used: Inhibition

    28) Product Images from "Allergen Component Specific IgE Measurement With the Immulite™ 2000 System: Diagnostic Accuracy and Intermethod Comparison"

    Article Title: Allergen Component Specific IgE Measurement With the Immulite™ 2000 System: Diagnostic Accuracy and Intermethod Comparison

    Journal: Journal of Clinical Laboratory Analysis

    doi: 10.1002/jcla.21741

    Scatterplot of intermethod comparison between Immulite™ 2000 and ImmunoCAP™ for Ole e 1, Bet v1, Bet v 2, Pru p 3, Pen m 1/Pen a 1, individually and altogether considered. Dotted line: 95% confidence interval.
    Figure Legend Snippet: Scatterplot of intermethod comparison between Immulite™ 2000 and ImmunoCAP™ for Ole e 1, Bet v1, Bet v 2, Pru p 3, Pen m 1/Pen a 1, individually and altogether considered. Dotted line: 95% confidence interval.

    Techniques Used:

    29) Product Images from "IgE Antibody Detection and Component Analysis in Patients with Eosinophilic Esophagitis"

    Article Title: IgE Antibody Detection and Component Analysis in Patients with Eosinophilic Esophagitis

    Journal: The journal of allergy and clinical immunology. In practice

    doi: 10.1016/j.jaip.2015.05.015

    Molecular allergen ImmunoCAP assays
    Figure Legend Snippet: Molecular allergen ImmunoCAP assays

    Techniques Used:

    Testing for IgE Antibody on diluted serum using ImmunoCAP
    Figure Legend Snippet: Testing for IgE Antibody on diluted serum using ImmunoCAP

    Techniques Used:

    Comparison of quantities of allergen extract-specific IgE measured using ImmunoCAP with semiquantitative ISAC standardized unit (ISU) levels of IgE antibody from ISAC testing for molecular allergens. Quantities of IgE antibody are distinguished by color:
    Figure Legend Snippet: Comparison of quantities of allergen extract-specific IgE measured using ImmunoCAP with semiquantitative ISAC standardized unit (ISU) levels of IgE antibody from ISAC testing for molecular allergens. Quantities of IgE antibody are distinguished by color:

    Techniques Used:

    The prevalence of sensitization to common whole food extracts in adults and children with EoE as determined by skin prick testing or ImmunoCAP testing (A). Levels of specific IgE antibody for those aeroallergens and foods to which patients with EoE have
    Figure Legend Snippet: The prevalence of sensitization to common whole food extracts in adults and children with EoE as determined by skin prick testing or ImmunoCAP testing (A). Levels of specific IgE antibody for those aeroallergens and foods to which patients with EoE have

    Techniques Used:

    Molecular allergen ImmunoCAP assays
    Figure Legend Snippet: Molecular allergen ImmunoCAP assays

    Techniques Used:

    30) Product Images from "Comparison of the ImmunoCAP Assay and AdvanSure™ AlloScreen Advanced Multiplex Specific IgE Detection Assay"

    Article Title: Comparison of the ImmunoCAP Assay and AdvanSure™ AlloScreen Advanced Multiplex Specific IgE Detection Assay

    Journal: Yonsei Medical Journal

    doi: 10.3349/ymj.2017.58.4.786

    Specific IgE titers of serially diluted serum specimens. Specific IgE titers of Dermatophagoides farinae ( D. farina ) detected by ImmunoCAP (A) and AlloScreen (B). Specific IgE titers of birch detected by ImmunoCAP (C) and AlloScreen (D). Specific IgE titers of dog detected by ImmunoCAP (E) and AlloScreen (F).
    Figure Legend Snippet: Specific IgE titers of serially diluted serum specimens. Specific IgE titers of Dermatophagoides farinae ( D. farina ) detected by ImmunoCAP (A) and AlloScreen (B). Specific IgE titers of birch detected by ImmunoCAP (C) and AlloScreen (D). Specific IgE titers of dog detected by ImmunoCAP (E) and AlloScreen (F).

    Techniques Used:

    Specific IgE titers of each allergen detected by ImmunoCAP and AlloScreen. D. pteronyssinus , Dermatophagoides pteronyssinus ; D. farinae , Dermatophagoides farinae .
    Figure Legend Snippet: Specific IgE titers of each allergen detected by ImmunoCAP and AlloScreen. D. pteronyssinus , Dermatophagoides pteronyssinus ; D. farinae , Dermatophagoides farinae .

    Techniques Used:

    31) Product Images from "Single Nanoparticle Detection for Multiplexed Protein Diagnostics with Attomolar Sensitivity in Serum and Unprocessed Whole Blood"

    Article Title: Single Nanoparticle Detection for Multiplexed Protein Diagnostics with Attomolar Sensitivity in Serum and Unprocessed Whole Blood

    Journal: Analytical chemistry

    doi: 10.1021/ac4000514

    Multiplexed assay to detect specific IgE to eight allergens. Fluorescence measurement was used as a control to validate nanoparticle counting detection ( R 2 = 0.97 for serum both and whole blood samples). Human sample was characterized with Phadia ImmunoCAP or skin prick testing (SPT): (+) reflects a positive result. Error bars correspond to the standard deviation from the mean ( n = 4). The * indicates that the results were not determined.
    Figure Legend Snippet: Multiplexed assay to detect specific IgE to eight allergens. Fluorescence measurement was used as a control to validate nanoparticle counting detection ( R 2 = 0.97 for serum both and whole blood samples). Human sample was characterized with Phadia ImmunoCAP or skin prick testing (SPT): (+) reflects a positive result. Error bars correspond to the standard deviation from the mean ( n = 4). The * indicates that the results were not determined.

    Techniques Used: Fluorescence, Single-particle Tracking, Standard Deviation

    32) Product Images from "Efficacy, safety and immunological actions of butanol-extracted FAHF-2 on peanut anaphylaxis"

    Article Title: Efficacy, safety and immunological actions of butanol-extracted FAHF-2 on peanut anaphylaxis

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    doi: 10.1111/j.1365-2222.2010.03643.x

    Effect of B-FAHF-2 on effector cells in vitro Effects of B-FAHF-2 on IL-4 (A) and IL-5(B) production by PN-primed SPCs were measured by ELISA. Cell viability (C) was assessed by Trypan Blue exclusion. Effect of B-FAHF-2 on degranulation of RBL 2H3 cells (D) was measured by β-hexoseaminidase release and cell viability (E) was assessed as above. Effect of B-FAHF-2 on IgE production by U266 cells (F) was measured by ImmunoCap. Data shown as Mean ± SD of triplicate cultures. ***, p
    Figure Legend Snippet: Effect of B-FAHF-2 on effector cells in vitro Effects of B-FAHF-2 on IL-4 (A) and IL-5(B) production by PN-primed SPCs were measured by ELISA. Cell viability (C) was assessed by Trypan Blue exclusion. Effect of B-FAHF-2 on degranulation of RBL 2H3 cells (D) was measured by β-hexoseaminidase release and cell viability (E) was assessed as above. Effect of B-FAHF-2 on IgE production by U266 cells (F) was measured by ImmunoCap. Data shown as Mean ± SD of triplicate cultures. ***, p

    Techniques Used: In Vitro, Enzyme-linked Immunosorbent Assay

    33) Product Images from "Comparison of ImmunoCAP and Immulite serum specific IgE assays for the assessment of egg allergy"

    Article Title: Comparison of ImmunoCAP and Immulite serum specific IgE assays for the assessment of egg allergy

    Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

    doi: 10.1186/s13223-016-0134-0

    Correlation plot of egg white specific IgE levels as measured with ImmunoCAP ® and Immulite ®
    Figure Legend Snippet: Correlation plot of egg white specific IgE levels as measured with ImmunoCAP ® and Immulite ®

    Techniques Used:

    34) Product Images from "New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis"

    Article Title: New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis

    Journal: Clinical and Vaccine Immunology : CVI

    doi: 10.1128/CVI.00498-15

    Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P
    Figure Legend Snippet: Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P

    Techniques Used: Immunocap ISAC Assay

    35) Product Images from "In Vitro Evaluation of Allergen Potencies of Commercial House Dust Mite Sublingual Immunotherapy Reagents"

    Article Title: In Vitro Evaluation of Allergen Potencies of Commercial House Dust Mite Sublingual Immunotherapy Reagents

    Journal: Allergy, Asthma & Immunology Research

    doi: 10.4168/aair.2015.7.2.124

    ImmunoCAP inhibition of D. farinae -specific IgE with house dust mite sublingual immunotherapy reagents and Hollister-Stier subcutaneous immunotherapy reagents. HS, Hollister-Stier; Wolwo, Wolwopharma ( D. farinae 15,000 AU/mL and D. pteronyssinus 15,000 AU/mL mixture).
    Figure Legend Snippet: ImmunoCAP inhibition of D. farinae -specific IgE with house dust mite sublingual immunotherapy reagents and Hollister-Stier subcutaneous immunotherapy reagents. HS, Hollister-Stier; Wolwo, Wolwopharma ( D. farinae 15,000 AU/mL and D. pteronyssinus 15,000 AU/mL mixture).

    Techniques Used: Inhibition

    Related Articles

    Modification:

    Article Title: The relevance of tick bites to the production of IgE antibodies to the mammalian oligosaccharide galactose-?-1,3-galactose
    Article Snippet: .. Total and specific IgE antibodies were measured by using either commercially available ImmunoCAP (Phadia US, Portage, MI) or a modification of the assay with streptavidin on the solid phase. ..

    Article Title: Seventeen-year-old girl hospitalized for localized swelling, pruritus, tenderness, and lymphatic streaking with eosinophilia
    Article Snippet: .. Total and specific IgE antibodies were measured by ImmunoCAP (Phadia US, Portage, Mich) or a modification of the assay with streptavidin on solid phase (Galactose-α-1,3-galactose, ladybug, and nitrophorin [cNP] protein ) ( ). ..

    Article Title: Galactose-?-1,3-Galactose-Specific IgE Is Associated with Anaphylaxis but Not Asthma
    Article Snippet: .. Total and specific IgE antibodies were measured by using either commercially available ImmunoCAP (Phadia US, Portage, MI) or a modification of the assay with streptavidin on the solid phase ( ). ..

    Article Title: The relationship between red meat allergy and sensitization to gelatin and galactose-alpha-1,3-galactose
    Article Snippet: .. Total and specific IgE antibodies were measured by using either commercially available ImmunoCAP (Phadia US, Portage, Mich, USA) or a modification of the assay with streptavidin on the solid phase as previously described ( , ). ..

    Article Title: Cetuximab-Induced Anaphylaxis and IgE Specific for Galactose-α-1,3-Galactose
    Article Snippet: .. ImmunoCAP is a variation of the radioallergosor-bent test in which IgE antibodies that have bound to antigen on the solid phase are detected with a secondary enzyme-labeled anti-IgE antibody., Total and specific IgE antibodies were measured with the use of either ImmunoCAP (Phadia U.S.) or the modified assay with streptavidin-coated ImmunoCAP. ..

    Acetylene Reduction Assay:

    Article Title: Diagnostic Value of Specific IgE to Peanut and Ara h 2 in Korean Children with Peanut Allergy
    Article Snippet: .. Serum samples were analyzed using immunoCAP (Thermo Fisher Scientific Inc., Waltham, MA, USA) to quantify total IgE and sIgE antibodies to peanuts, Ara h 1, Ara h 2, Ara h 3, Ara h 8, and Ara h 9. ..

    other:

    Article Title: Domestic airborne black carbon and exhaled nitric oxide in children in NYC
    Article Snippet: Serum IgE antibodies against German cockroach, mouse urine, D. farinea , cat dander, dog dander, common ragweed, and mixed tree (Tx8) and grass (Gx2) pollen were measured by ImmunoCAP (Phadia, Uppsala, Sweden).

    Article Title: Intranasal administration of allergen increases specific IgE whereas intranasal omalizumab does not increase serum IgE levels—A pilot study, et al. Intranasal administration of allergen increases specific IgE whereas intranasal omalizumab does not increase serum IgE levels —A pilot study
    Article Snippet: Supernatants were taken after 1 week of culture and frozen at −20°C until measurement of total IgE levels by ImmunoCAP (total IgE low range, Phadia‐Thermofisher, Uppsala, Sweden).

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    Thermo Fisher igg4
    Component- and peanut-specific immunoglobulin data over time on treatment for all subjects. A) Specific IgE; B) Specific <t>IgG4;</t> C) Specific IgE/specific IgG4. *p
    Igg4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunocap feia
    Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by <t>ImmunoCAP-FEIA</t> in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.
    Immunocap Feia, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunocap
    No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via <t>ImmunoCAP.</t> Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p
    Immunocap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Component- and peanut-specific immunoglobulin data over time on treatment for all subjects. A) Specific IgE; B) Specific IgG4; C) Specific IgE/specific IgG4. *p

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    Article Title: Utility of component analyses in subjects undergoing sublingual immunotherapy for peanut allergy

    doi: 10.1111/cea.12635

    Figure Lengend Snippet: Component- and peanut-specific immunoglobulin data over time on treatment for all subjects. A) Specific IgE; B) Specific IgG4; C) Specific IgE/specific IgG4. *p

    Article Snippet: Peanut- and peanut component-specific IgE and IgG4 were measured via ImmunoCAP 100 (Thermo Fisher, Uppsala, Sweden) according to the manufacturer’s specifications.

    Techniques:

    Baseline component- and peanut-specific immunoglobulin data for subjects passing (P) and failing (F) the 12 month food challenge. A) Specific IgE; B) Specific IgG4; C) Specific IgE/Specific IgG4. *p

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    Article Title: Utility of component analyses in subjects undergoing sublingual immunotherapy for peanut allergy

    doi: 10.1111/cea.12635

    Figure Lengend Snippet: Baseline component- and peanut-specific immunoglobulin data for subjects passing (P) and failing (F) the 12 month food challenge. A) Specific IgE; B) Specific IgG4; C) Specific IgE/Specific IgG4. *p

    Article Snippet: Peanut- and peanut component-specific IgE and IgG4 were measured via ImmunoCAP 100 (Thermo Fisher, Uppsala, Sweden) according to the manufacturer’s specifications.

    Techniques:

    Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by ImmunoCAP-FEIA in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.

    Journal: Scientific Reports

    Article Title: Differential Plasma-cell evolution is linked with Dermatophagoides pteronyssinus immunotherapy response

    doi: 10.1038/srep14482

    Figure Lengend Snippet: Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by ImmunoCAP-FEIA in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.

    Article Snippet: Total and specific IgE, IgG4 assays Total and specific IgE and IgG4 to DP were determined by ImmunoCAP-FEIA in serum samples, according to manufacturer instructions (Thermo Fisher Scientific, Massachusetts, USA).

    Techniques: Enzyme-linked Immunospot, MANN-WHITNEY

    No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via ImmunoCAP. Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p

    Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    Article Title: Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects

    doi: 10.1111/pai.12310

    Figure Lengend Snippet: No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via ImmunoCAP. Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p

    Article Snippet: We included (randomized) 31 patients, aged 2–5 yr, with a sensitization to house-dust mite or grass pollen as defined either by skin prick test (ALK-Abelló, Hamburg, Germany) or by specific IgE antibody testing (ImmunoCAP® ; Thermo Fisher Scientific-Phadia AB, Uppsala, Sweden) ( ).

    Techniques: Microarray

    Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    Article Title: Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects

    doi: 10.1111/pai.12310

    Figure Lengend Snippet: Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Article Snippet: We included (randomized) 31 patients, aged 2–5 yr, with a sensitization to house-dust mite or grass pollen as defined either by skin prick test (ALK-Abelló, Hamburg, Germany) or by specific IgE antibody testing (ImmunoCAP® ; Thermo Fisher Scientific-Phadia AB, Uppsala, Sweden) ( ).

    Techniques: Microarray