immunocap system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher immunocap system
    The Box-and-Whisker plot of fungal spore concentration (CFU/m 3 ) in the active and inactive stages among patients with positive <t>ImmunoCAP</t> test results for specific IgE to the designated fungal allergens. (A) Cladosporium oxysporum , (B) Cladosporium cladosporioides , (C) Cladosporium spp, (D) Aspergillus niger , (E) Penicillium brevicompactum , and (F) Penicillium oxalicum .
    Immunocap System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunocap system/product/Thermo Fisher
    Average 95 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    immunocap system - by Bioz Stars, 2020-05
    95/100 stars

    Images

    1) Product Images from "Does Spore Count Matter in Fungal Allergy?: The Role of Allergenic Fungal Species"

    Article Title: Does Spore Count Matter in Fungal Allergy?: The Role of Allergenic Fungal Species

    Journal: Allergy, Asthma & Immunology Research

    doi: 10.4168/aair.2016.8.5.404

    The Box-and-Whisker plot of fungal spore concentration (CFU/m 3 ) in the active and inactive stages among patients with positive ImmunoCAP test results for specific IgE to the designated fungal allergens. (A) Cladosporium oxysporum , (B) Cladosporium cladosporioides , (C) Cladosporium spp, (D) Aspergillus niger , (E) Penicillium brevicompactum , and (F) Penicillium oxalicum .
    Figure Legend Snippet: The Box-and-Whisker plot of fungal spore concentration (CFU/m 3 ) in the active and inactive stages among patients with positive ImmunoCAP test results for specific IgE to the designated fungal allergens. (A) Cladosporium oxysporum , (B) Cladosporium cladosporioides , (C) Cladosporium spp, (D) Aspergillus niger , (E) Penicillium brevicompactum , and (F) Penicillium oxalicum .

    Techniques Used: Whisker Assay, Concentration Assay

    2) Product Images from "High-Yield Production of the Major Birch Pollen Allergen Bet v 1 With Allergen Immunogenicity in Nicotiana benthamiana"

    Article Title: High-Yield Production of the Major Birch Pollen Allergen Bet v 1 With Allergen Immunogenicity in Nicotiana benthamiana

    Journal: Frontiers in Plant Science

    doi: 10.3389/fpls.2020.00344

    Correlation between specific recombinant Bet v 1 IgE levels determined by ImmunoCAP and OD values in ELISA assays using recombinant allergens derived from N. benthamiana (A) or B. brevis (B) . Rho = 0.91, P = 0.0001 for N. benthamiana and rho = 0.95, P
    Figure Legend Snippet: Correlation between specific recombinant Bet v 1 IgE levels determined by ImmunoCAP and OD values in ELISA assays using recombinant allergens derived from N. benthamiana (A) or B. brevis (B) . Rho = 0.91, P = 0.0001 for N. benthamiana and rho = 0.95, P

    Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay, Derivative Assay

    3) Product Images from "Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis"

    Article Title: Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis

    Journal: Allergo Journal International

    doi: 10.1007/s40629-015-0046-4

    Comparison of sIgE results obtained with the ImmunoCAP ISAC test with 8 extract-based ImmunoCAP singelplex tests (n = 101).
    Figure Legend Snippet: Comparison of sIgE results obtained with the ImmunoCAP ISAC test with 8 extract-based ImmunoCAP singelplex tests (n = 101).

    Techniques Used:

    4) Product Images from "Dog saliva - an important source of dog allergens"

    Article Title: Dog saliva - an important source of dog allergens

    Journal: Allergy

    doi: 10.1111/all.12130

    IgE reactivity. (A) Correlation between IgE reactivity to dog dander by ELISA ( y- axis) and ImmunoCAP (e5) ( x- axis) ( n = 20); (B) correlation between IgE reactivity in ELISA to dog dander (y- axis) and dog saliva ( x- axis) ( n = 59); OD – optical density, r 2 – correlation factor.
    Figure Legend Snippet: IgE reactivity. (A) Correlation between IgE reactivity to dog dander by ELISA ( y- axis) and ImmunoCAP (e5) ( x- axis) ( n = 20); (B) correlation between IgE reactivity in ELISA to dog dander (y- axis) and dog saliva ( x- axis) ( n = 59); OD – optical density, r 2 – correlation factor.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    5) Product Images from "Advances in allergen-microarray technology for diagnosis and monitoring of allergy: The MeDALL allergen-chip"

    Article Title: Advances in allergen-microarray technology for diagnosis and monitoring of allergy: The MeDALL allergen-chip

    Journal: Methods (San Diego, Calif.)

    doi: 10.1016/j.ymeth.2013.10.008

    Measurement of monoclonal human Bet v 1-specific IgE antibody levels using the MeDALL allergen-chip and by ImmunoCAP. Different concentrations of a monoclonal human IgE antibody specific to Bet v 1 ( x -axes) were tested. Bet v 1-specific IgE-levels measured
    Figure Legend Snippet: Measurement of monoclonal human Bet v 1-specific IgE antibody levels using the MeDALL allergen-chip and by ImmunoCAP. Different concentrations of a monoclonal human IgE antibody specific to Bet v 1 ( x -axes) were tested. Bet v 1-specific IgE-levels measured

    Techniques Used: Chromatin Immunoprecipitation

    3.2.2. Background, cut-off and inter-assay variation of the MeDALL allergen-chip for IgE in comparison to ImmunoCAP, and for IgG-detection
    Figure Legend Snippet: 3.2.2. Background, cut-off and inter-assay variation of the MeDALL allergen-chip for IgE in comparison to ImmunoCAP, and for IgG-detection

    Techniques Used: Inter Assay, Chromatin Immunoprecipitation

    3.2.3. Simultaneous presence of blocking IgG antibodies inhibits IgE binding to allergens on the chip but not in the ImmunoCAP
    Figure Legend Snippet: 3.2.3. Simultaneous presence of blocking IgG antibodies inhibits IgE binding to allergens on the chip but not in the ImmunoCAP

    Techniques Used: Blocking Assay, Binding Assay, Chromatin Immunoprecipitation

    6) Product Images from "Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure"

    Article Title: Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure

    Journal: Annals of Laboratory Medicine

    doi: 10.3343/alm.2018.38.4.338

    Correlation analysis of three in vitro diagnostic platforms: GOLD chip, ImmunoCAP, and AdvanSure. The sera of 150 patients with allergy were screened for seven of the most common allergens, and the amount of allergen-specific IgE (sIgE) measured by each platform was plotted for Pearson's correlation analysis.
    Figure Legend Snippet: Correlation analysis of three in vitro diagnostic platforms: GOLD chip, ImmunoCAP, and AdvanSure. The sera of 150 patients with allergy were screened for seven of the most common allergens, and the amount of allergen-specific IgE (sIgE) measured by each platform was plotted for Pearson's correlation analysis.

    Techniques Used: In Vitro, Diagnostic Assay, Chromatin Immunoprecipitation

    7) Product Images from "New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis"

    Article Title: New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis

    Journal: Clinical and Vaccine Immunology : CVI

    doi: 10.1128/CVI.00498-15

    Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P
    Figure Legend Snippet: Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P

    Techniques Used: Immunocap ISAC Assay

    8) Product Images from "Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing"

    Article Title: Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0191987

    Example ImmunoCAP ® -correlation of AllerBead with and without PC-PURE. Correlation plot of the multiplex AllerBead assays with and without PC-PURE, compared to the standard, FDA-cleared, non-multiplex ImmunoCAP ® test (for the tree nut cashew) for all 205 Boston Children’s Hospital patients. Pearson’s r and slope of the linear regression lines are provided. Note that AllerBead results were converted to kIU A /L by heterologous interpolation from a standard curve (5 points; R 2 of linear regression = 0.99) comprised of purified IgE from the serum of patients with various known amounts of sIgE (based on ImmunoCAP ® testing). Pearson’s r for all foods are shown in Fig 4b .
    Figure Legend Snippet: Example ImmunoCAP ® -correlation of AllerBead with and without PC-PURE. Correlation plot of the multiplex AllerBead assays with and without PC-PURE, compared to the standard, FDA-cleared, non-multiplex ImmunoCAP ® test (for the tree nut cashew) for all 205 Boston Children’s Hospital patients. Pearson’s r and slope of the linear regression lines are provided. Note that AllerBead results were converted to kIU A /L by heterologous interpolation from a standard curve (5 points; R 2 of linear regression = 0.99) comprised of purified IgE from the serum of patients with various known amounts of sIgE (based on ImmunoCAP ® testing). Pearson’s r for all foods are shown in Fig 4b .

    Techniques Used: Multiplex Assay, Purification

    Concentrating patient IgE with PC-PURE: Increased low-end sensitivity for sIgE. PC-PURE was used to simultaneously purify and concentrate IgE from 46 food allergy samples, followed by analysis on the multiplex AllerBead assay. To achieve the concentrating effect, the input sample volume for the PC-PURE step was 500 μL and the photo-release volume was 100 μL (“5x”), which was then input into the AllerBead assay. This was compared to PC-PURE used to only purify but not concentrate the IgE (“1x”; 100 μL input and photo-release volumes). AllerBead sensitivity (percent of sIgE-positives detected) was assessed in the low-end of the ImmunoCAP ® scale, defined as between 0.35 kIU A /L and 5 kIU A /L (sIgE positives determined a priori by ImmunoCAP ® testing).
    Figure Legend Snippet: Concentrating patient IgE with PC-PURE: Increased low-end sensitivity for sIgE. PC-PURE was used to simultaneously purify and concentrate IgE from 46 food allergy samples, followed by analysis on the multiplex AllerBead assay. To achieve the concentrating effect, the input sample volume for the PC-PURE step was 500 μL and the photo-release volume was 100 μL (“5x”), which was then input into the AllerBead assay. This was compared to PC-PURE used to only purify but not concentrate the IgE (“1x”; 100 μL input and photo-release volumes). AllerBead sensitivity (percent of sIgE-positives detected) was assessed in the low-end of the ImmunoCAP ® scale, defined as between 0.35 kIU A /L and 5 kIU A /L (sIgE positives determined a priori by ImmunoCAP ® testing).

    Techniques Used: Multiplex Assay

    Performance metrics of AllerBead with and without PC-PURE. Serum samples from 205 subjects presenting at Boston Children’s Hospital with suspicion of or known food allergy were analyzed by the multiplex AllerBead assay against all eight food allergens under study. AllerBead was performed with and without PC-PURE. Results from the standard, FDA-cleared, non-multiplex ImmunoCAP ® test for all eight foods were used as a reference and to determine true positives and negatives for sIgE. (a) Signal-to-Noise of AllerBead. Signal-to-noise was calculated on a per-food basis as the average AllerBead result for ImmunoCAP ® -positives (≥0.10 kIU A /L) divided by the average AllerBead result of ImmunoCAP ® -negatives (
    Figure Legend Snippet: Performance metrics of AllerBead with and without PC-PURE. Serum samples from 205 subjects presenting at Boston Children’s Hospital with suspicion of or known food allergy were analyzed by the multiplex AllerBead assay against all eight food allergens under study. AllerBead was performed with and without PC-PURE. Results from the standard, FDA-cleared, non-multiplex ImmunoCAP ® test for all eight foods were used as a reference and to determine true positives and negatives for sIgE. (a) Signal-to-Noise of AllerBead. Signal-to-noise was calculated on a per-food basis as the average AllerBead result for ImmunoCAP ® -positives (≥0.10 kIU A /L) divided by the average AllerBead result of ImmunoCAP ® -negatives (

    Techniques Used: Multiplex Assay

    9) Product Images from "Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing"

    Article Title: Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0191987

    Concentrating patient IgE with PC-PURE: Increased low-end sensitivity for sIgE. PC-PURE was used to simultaneously purify and concentrate IgE from 46 food allergy samples, followed by analysis on the multiplex AllerBead assay. To achieve the concentrating effect, the input sample volume for the PC-PURE step was 500 μL and the photo-release volume was 100 μL (“5x”), which was then input into the AllerBead assay. This was compared to PC-PURE used to only purify but not concentrate the IgE (“1x”; 100 μL input and photo-release volumes). AllerBead sensitivity (percent of sIgE-positives detected) was assessed in the low-end of the ImmunoCAP ® scale, defined as between 0.35 kIU A /L and 5 kIU A /L (sIgE positives determined a priori by ImmunoCAP ® testing).
    Figure Legend Snippet: Concentrating patient IgE with PC-PURE: Increased low-end sensitivity for sIgE. PC-PURE was used to simultaneously purify and concentrate IgE from 46 food allergy samples, followed by analysis on the multiplex AllerBead assay. To achieve the concentrating effect, the input sample volume for the PC-PURE step was 500 μL and the photo-release volume was 100 μL (“5x”), which was then input into the AllerBead assay. This was compared to PC-PURE used to only purify but not concentrate the IgE (“1x”; 100 μL input and photo-release volumes). AllerBead sensitivity (percent of sIgE-positives detected) was assessed in the low-end of the ImmunoCAP ® scale, defined as between 0.35 kIU A /L and 5 kIU A /L (sIgE positives determined a priori by ImmunoCAP ® testing).

    Techniques Used: Multiplex Assay

    10) Product Images from "Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure"

    Article Title: Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure

    Journal: Annals of Laboratory Medicine

    doi: 10.3343/alm.2018.38.4.338

    Correlation analysis of three in vitro diagnostic platforms: GOLD chip, ImmunoCAP, and AdvanSure. The sera of 150 patients with allergy were screened for seven of the most common allergens, and the amount of allergen-specific IgE (sIgE) measured by each platform was plotted for Pearson's correlation analysis.
    Figure Legend Snippet: Correlation analysis of three in vitro diagnostic platforms: GOLD chip, ImmunoCAP, and AdvanSure. The sera of 150 patients with allergy were screened for seven of the most common allergens, and the amount of allergen-specific IgE (sIgE) measured by each platform was plotted for Pearson's correlation analysis.

    Techniques Used: In Vitro, Diagnostic Assay, Chromatin Immunoprecipitation

    11) Product Images from "New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis"

    Article Title: New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis

    Journal: Clinical and Vaccine Immunology : CVI

    doi: 10.1128/CVI.00498-15

    Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P
    Figure Legend Snippet: Correlation between ImmunoCAP assay and DELFIA (Spearman's rank test, P

    Techniques Used: Immunocap ISAC Assay

    Related Articles

    Multiplex Assay:

    Article Title: Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis
    Article Snippet: .. IgE Analysis IgE analysis of serum samples was performed (A) traditional extract-based singleplex-testing with the ImmunoCAP system and (B) with the molecular multiplex test ImmunoCAP® ISAC 112 (both Thermo Fisher Scientific, Uppsala, Sweden). ..

    RAST Test:

    Article Title: Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure
    Article Snippet: .. The currently available tests include the radioallergosorbent test (RAST), multiple allergen simultaneous test (MAST), and ImmunoCAP system (Thermo Scientific, Uppsala, Sweden). .. The once market-leading RAST method is no longer popular, since this is considered to be a rather inefficient and expensive method compared with others [ ].

    Binding Assay:

    Article Title: Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing
    Article Snippet: .. The current standard approach is the ImmunoCAP® system (Phadia/Thermo Fisher Scientific), which measures the concentration of circulating allergen-specific IgE antibodies (sIgE) in human serum or plasma (by measuring sIgE binding to allergen immobilized on an assay surface, using a fluorescence enzyme immunoassay [FEIA] format). ..

    Fluorescence:

    Article Title: Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing
    Article Snippet: .. The current standard approach is the ImmunoCAP® system (Phadia/Thermo Fisher Scientific), which measures the concentration of circulating allergen-specific IgE antibodies (sIgE) in human serum or plasma (by measuring sIgE binding to allergen immobilized on an assay surface, using a fluorescence enzyme immunoassay [FEIA] format). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing
    Article Snippet: .. The current standard approach is the ImmunoCAP® system (Phadia/Thermo Fisher Scientific), which measures the concentration of circulating allergen-specific IgE antibodies (sIgE) in human serum or plasma (by measuring sIgE binding to allergen immobilized on an assay surface, using a fluorescence enzyme immunoassay [FEIA] format). ..

    Article Title: High-Yield Production of the Major Birch Pollen Allergen Bet v 1 With Allergen Immunogenicity in Nicotiana benthamiana
    Article Snippet: .. IgE reactivity to recombinant Bet v 1 from N. benthamiana and B. brevis was determined by ELISA using 12 serum samples obtained from a general population, and these values were then compared with those measured by ImmunoCAP system, a standard specific IgE detection method (specific IgE test for Bet v 1, Thermo Fisher Diagnostics K.K., Tokyo, Japan). .. Recombinant Bet v 1 produced in Escherichia coli was used to determine specific IgE levels for Bet v 1. shows the correlation between the level of specific Bet v1 IgE determined by ImmunoCAP and the OD values from ELISA assays using recombinant allergens derived from N. benthamiana ( ) and those between ImmnoCAP values and OD values from B. brevis ( ).

    Concentration Assay:

    Article Title: Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing
    Article Snippet: .. The current standard approach is the ImmunoCAP® system (Phadia/Thermo Fisher Scientific), which measures the concentration of circulating allergen-specific IgE antibodies (sIgE) in human serum or plasma (by measuring sIgE binding to allergen immobilized on an assay surface, using a fluorescence enzyme immunoassay [FEIA] format). ..

    Recombinant:

    Article Title: High-Yield Production of the Major Birch Pollen Allergen Bet v 1 With Allergen Immunogenicity in Nicotiana benthamiana
    Article Snippet: .. IgE reactivity to recombinant Bet v 1 from N. benthamiana and B. brevis was determined by ELISA using 12 serum samples obtained from a general population, and these values were then compared with those measured by ImmunoCAP system, a standard specific IgE detection method (specific IgE test for Bet v 1, Thermo Fisher Diagnostics K.K., Tokyo, Japan). .. Recombinant Bet v 1 produced in Escherichia coli was used to determine specific IgE levels for Bet v 1. shows the correlation between the level of specific Bet v1 IgE determined by ImmunoCAP and the OD values from ELISA assays using recombinant allergens derived from N. benthamiana ( ) and those between ImmnoCAP values and OD values from B. brevis ( ).

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    Thermo Fisher immunocap calibrators
    <t>ImmunoCAP</t> ® and biosensor calibration curves. ( A ) ImmunoCAP ® curve, range 2–5000 kU/L; ( B ) ImmunoCAP ® curve, range 0.35–100 kU/L; ( C ) Biochip curve, range 2–5000 kU/L (point-of-care (PoC) reader platform signal); ( D ) Biochip curve, range 0.7–1000 kU/L (PoC reader platform signal).
    Immunocap Calibrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunocap calibrators/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunocap calibrators - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher immunocap feia
    Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by <t>ImmunoCAP-FEIA</t> in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.
    Immunocap Feia, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunocap feia/product/Thermo Fisher
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    immunocap feia - by Bioz Stars, 2020-05
    88/100 stars
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    95
    Thermo Fisher immunocap
    No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via <t>ImmunoCAP.</t> Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p
    Immunocap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunocap/product/Thermo Fisher
    Average 95 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    immunocap - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    ImmunoCAP ® and biosensor calibration curves. ( A ) ImmunoCAP ® curve, range 2–5000 kU/L; ( B ) ImmunoCAP ® curve, range 0.35–100 kU/L; ( C ) Biochip curve, range 2–5000 kU/L (point-of-care (PoC) reader platform signal); ( D ) Biochip curve, range 0.7–1000 kU/L (PoC reader platform signal).

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Proof-of-Concept of Label-Free Biosensing System for Food Allergy Diagnostics in Biophotonic Sensing Cells: Performance Comparison with ImmunoCAP

    doi: 10.3390/s18082686

    Figure Lengend Snippet: ImmunoCAP ® and biosensor calibration curves. ( A ) ImmunoCAP ® curve, range 2–5000 kU/L; ( B ) ImmunoCAP ® curve, range 0.35–100 kU/L; ( C ) Biochip curve, range 2–5000 kU/L (point-of-care (PoC) reader platform signal); ( D ) Biochip curve, range 0.7–1000 kU/L (PoC reader platform signal).

    Article Snippet: Finally, we measured the recognition response in increasing levels of human IgEs molecules provided by ImmunoCAP® calibrators (ThermoFisher Scientific, Phadia AB, Uppsala, Sweden) and compared both recognition responses (i.e., ImmunoCAP® and our system responses).

    Techniques:

    Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by ImmunoCAP-FEIA in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.

    Journal: Scientific Reports

    Article Title: Differential Plasma-cell evolution is linked with Dermatophagoides pteronyssinus immunotherapy response

    doi: 10.1038/srep14482

    Figure Lengend Snippet: Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by ImmunoCAP-FEIA in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.

    Article Snippet: Total and specific IgE, IgG4 assays Total and specific IgE and IgG4 to DP were determined by ImmunoCAP-FEIA in serum samples, according to manufacturer instructions (Thermo Fisher Scientific, Massachusetts, USA).

    Techniques: Enzyme-linked Immunospot, MANN-WHITNEY

    No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via ImmunoCAP. Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p

    Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    Article Title: Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects

    doi: 10.1111/pai.12310

    Figure Lengend Snippet: No evidence for an enhanced or reduced IgE sensitization rate in patients treated with preventive sublingual immunotherapy (SLIT). (a) The number of sensitizations to a panel of allergens (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk) was assessed via ImmunoCAP. Each patient was assigned as monosensitized (one sensitization), oligosensitized (2–3 sensitizations), and polysensitized ( > 4 sensitizations). Changes over treatment period are shown. (b) Changes of IgE sensitizations to the 10 allergen sources are shown over treatment period. (c) Microarray analysis of IgE sensitization was used to assess specific IgE antibodies to 58 components of 10 allergen sources (Dermatophagoides pteronyssinus, 6-grass–pollen mix, birch pollen, cladosporium herbarum, alternaria alternata, peanut, cat- and dog dander, hen’s egg, and cow’s milk). Each dot represents one patient’s serum recognizing an individual number of allergen components of the 10 allergen sources. p

    Article Snippet: We included (randomized) 31 patients, aged 2–5 yr, with a sensitization to house-dust mite or grass pollen as defined either by skin prick test (ALK-Abelló, Hamburg, Germany) or by specific IgE antibody testing (ImmunoCAP® ; Thermo Fisher Scientific-Phadia AB, Uppsala, Sweden) ( ).

    Techniques: Microarray

    Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    Article Title: Preventive sublingual immunotherapy in preschool children: First evidence for safety and pro-tolerogenic effects

    doi: 10.1111/pai.12310

    Figure Lengend Snippet: Specific IgG but not specific IgE to house-dust mite allergens significantly increases during preventive sublingual immunotherapy (SLIT). Specific IgE to Der p 1 and Der p 2 was measured by ImmunoCAP. Specific IgG was measured by microarray technology. *p

    Article Snippet: We included (randomized) 31 patients, aged 2–5 yr, with a sensitization to house-dust mite or grass pollen as defined either by skin prick test (ALK-Abelló, Hamburg, Germany) or by specific IgE antibody testing (ImmunoCAP® ; Thermo Fisher Scientific-Phadia AB, Uppsala, Sweden) ( ).

    Techniques: Microarray

    A and B , Likelihood ( y-axis , percentage) of reporting symptoms to cat at 16 years of age depending of the number of IgE-reactive cat allergens (Fel d 1, Fel d 2, and Fel d 4) and ImmunoCAP cat extract sensitization (x-axes) at 4 (Fig 5, A ) and 8 (Fig 5, B ) years of age. C and D , Likelihood ( y-axis , percentage) of reporting symptoms to dog at 16 years of age depending of the number of IgE-reactive dog allergens (Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6) and ImmunoCAP dog extract sensitization (x-axes) at 4 (Fig 5, C ) and 8 (Fig 5, D ) years of age. Numbers, ORs, and 95% CIs are shown (n = 779).

    Journal: The Journal of allergy and clinical immunology

    Article Title: Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study

    doi: 10.1016/j.jaci.2015.09.052

    Figure Lengend Snippet: A and B , Likelihood ( y-axis , percentage) of reporting symptoms to cat at 16 years of age depending of the number of IgE-reactive cat allergens (Fel d 1, Fel d 2, and Fel d 4) and ImmunoCAP cat extract sensitization (x-axes) at 4 (Fig 5, A ) and 8 (Fig 5, B ) years of age. C and D , Likelihood ( y-axis , percentage) of reporting symptoms to dog at 16 years of age depending of the number of IgE-reactive dog allergens (Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6) and ImmunoCAP dog extract sensitization (x-axes) at 4 (Fig 5, C ) and 8 (Fig 5, D ) years of age. Numbers, ORs, and 95% CIs are shown (n = 779).

    Article Snippet: Serum samples were initially tested with ImmunoCAP (Thermo Fisher AB, Uppsala, Sweden) for allergen-specific IgE antibodies to cat (e1) and dog (e5) extract.

    Techniques: