free psa  (Beckman Coulter)


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    Structured Review

    Beckman Coulter free psa
    Calibration curves of transition 636.8/943.5 monitored for peptide LSEPAELTDAVK derived from <t>PSA.</t> The spiking concentrations were 0.5, 1, 1.5, 2.5, 5, 7.5, 10, 25, and 50 ng/mL for both the <t>IgY14</t> (A) and IgY14/SuperMix (B) based workflows for measurements
    Free Psa, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 89/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/free psa/product/Beckman Coulter
    Average 89 stars, based on 272 article reviews
    Price from $9.99 to $1999.99
    free psa - by Bioz Stars, 2020-09
    89/100 stars

    Images

    1) Product Images from "Analysis of Serum Total and Free PSA Using Immunoaffinity Depletion Coupled to SRM: Correlation with Clinical Immunoassay Tests"

    Article Title: Analysis of Serum Total and Free PSA Using Immunoaffinity Depletion Coupled to SRM: Correlation with Clinical Immunoassay Tests

    Journal: Journal of proteomics

    doi: 10.1016/j.jprot.2012.01.035

    Calibration curves of transition 636.8/943.5 monitored for peptide LSEPAELTDAVK derived from PSA. The spiking concentrations were 0.5, 1, 1.5, 2.5, 5, 7.5, 10, 25, and 50 ng/mL for both the IgY14 (A) and IgY14/SuperMix (B) based workflows for measurements
    Figure Legend Snippet: Calibration curves of transition 636.8/943.5 monitored for peptide LSEPAELTDAVK derived from PSA. The spiking concentrations were 0.5, 1, 1.5, 2.5, 5, 7.5, 10, 25, and 50 ng/mL for both the IgY14 (A) and IgY14/SuperMix (B) based workflows for measurements

    Techniques Used: Derivative Assay

    Re-constructed calibration curves for (A) total and (B) free PSA measurements using the IgY14 and IgY14/SuperMix based workflows, respectively. The PSA concentrations measured by the total and free PSA immunoassays, instead of the nominal PSA spiking
    Figure Legend Snippet: Re-constructed calibration curves for (A) total and (B) free PSA measurements using the IgY14 and IgY14/SuperMix based workflows, respectively. The PSA concentrations measured by the total and free PSA immunoassays, instead of the nominal PSA spiking

    Techniques Used: Construct

    2) Product Images from "tRNA Is the Source of Low-Level trans-Zeatin Production in Methylobacterium spp. † spp. † ‡"

    Article Title: tRNA Is the Source of Low-Level trans-Zeatin Production in Methylobacterium spp. † spp. † ‡

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.184.7.1832-1842.2002

    RIA of the M. extorquens tRNA hydrolysate purified by anti- cis -zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis -zeatin (cZR) is indicated by a bar.
    Figure Legend Snippet: RIA of the M. extorquens tRNA hydrolysate purified by anti- cis -zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis -zeatin (cZR) is indicated by a bar.

    Techniques Used: Purification

    RIA analysis (A) and HPLC absorbance profile (B) of cytokinins isolated by the immunoaffinity procedure from spent media from cultures of the Arabidopsis PPFM isolate. The retention times of trans -zeatin (Z) and trans- zeatin riboside (ZR) standards are indicated by bars. Control media showed no activity in any fraction. The data were not corrected for recovery.
    Figure Legend Snippet: RIA analysis (A) and HPLC absorbance profile (B) of cytokinins isolated by the immunoaffinity procedure from spent media from cultures of the Arabidopsis PPFM isolate. The retention times of trans -zeatin (Z) and trans- zeatin riboside (ZR) standards are indicated by bars. Control media showed no activity in any fraction. The data were not corrected for recovery.

    Techniques Used: High Performance Liquid Chromatography, Isolation, Activity Assay

    3) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

    Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

    Journal: Journal of Proteome Research

    doi: 10.1021/pr800904z

    General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.
    Figure Legend Snippet: General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.

    Techniques Used: Labeling, Electrofocusing, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    4) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

    Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

    Journal: Journal of Proteome Research

    doi: 10.1021/pr800904z

    General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.
    Figure Legend Snippet: General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.

    Techniques Used: Labeling, Electrofocusing, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    5) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

    Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

    Journal: Journal of Proteome Research

    doi: 10.1021/pr800904z

    General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.
    Figure Legend Snippet: General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.

    Techniques Used: Labeling, Electrofocusing, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berries
    Article Snippet: .. The metabolites were separated by HPLC using a System Gold HPLC with the software 32 Karat (Beckman Coulter, NSW, Australia). .. Aliquots of the post-column eluate were collected into 5 ml scintillation vials according to the peaks observed on a flatbed recorder (Kipp and Zonen, Delft, The Netherlands) directly connected to the photodiode array detector.

    Article Title: Effects of secondary metabolites produced by different cyanobacterial populations on the freshwater zooplankters Brachionus calyciflorus and Daphnia pulex
    Article Snippet: .. HPLC-FLD analysis of anatoxin-a ANTX was determined using HPLC with fluorescence detection (FLD, Beckman). .. Ten microliters of each extract was reconstituted with 100 μl of 0.1 M sodium borate in a 2-mL Eppendorf probe.

    Article Title: tRNA Is the Source of Low-Level trans-Zeatin Production in Methylobacterium spp. † spp. † ‡
    Article Snippet: .. The immunoaffinity-purified cytokinins were fractionated by HPLC performed with a Beckman Ultrasphere octadecylsilica column (5 μm; 4.6 by 250 mm) equilibrated in triethylammonium acetate buffer (40 mM acetic acid adjusted to pH 3.4 with triethylamine [ ]). .. They were eluted at a flow rate of 1.0 ml/min with a linear acetonitrile gradient (the initial gradient was either 5 to 15% or 10 to 15% for 15 min and was followed by a 15 to 35% gradient for 15 min and a 35 to 100% gradient for 1 min).

    Centrifugation:

    Article Title: Coproduction of Acetaldehyde and Hydrogen during Glucose Fermentation by Escherichia coli ▿ ▿ †
    Article Snippet: .. Cells were harvested by centrifugation at 10,000 × g using an Avanti J-25 centrifuge and a JA-17 rotor (Beckman Coulter, Brea, CA). .. Cell pellets were lysed with bacterial protein extraction reagent (BPER) (Thermo Scientific), and the lysate was centrifuged to separate the supernatant from the pellet according to the manufacturer's instructions.

    other:

    Article Title: The Prostate Health Index Selectively Identifies Clinically Significant Prostate Cancer
    Article Snippet: Free PSA is, in fact, comprised of several different iso forms including [-2]proPSA, which is more specific for prostate cancer than total PSA or free PSA., The Beckman Coulter Prostate Health Index combines total, free and [-2]proPSA into a single score.

    Fluorescence:

    Article Title: Effects of secondary metabolites produced by different cyanobacterial populations on the freshwater zooplankters Brachionus calyciflorus and Daphnia pulex
    Article Snippet: .. HPLC-FLD analysis of anatoxin-a ANTX was determined using HPLC with fluorescence detection (FLD, Beckman). .. Ten microliters of each extract was reconstituted with 100 μl of 0.1 M sodium borate in a 2-mL Eppendorf probe.

    Software:

    Article Title: Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berries
    Article Snippet: .. The metabolites were separated by HPLC using a System Gold HPLC with the software 32 Karat (Beckman Coulter, NSW, Australia). .. Aliquots of the post-column eluate were collected into 5 ml scintillation vials according to the peaks observed on a flatbed recorder (Kipp and Zonen, Delft, The Netherlands) directly connected to the photodiode array detector.

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    Beckman Coulter immunoaffinity column
    General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by <t>immunoaffinity</t> depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.
    Immunoaffinity Column, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column/product/Beckman Coulter
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity column - by Bioz Stars, 2020-09
    91/100 stars
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    General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.

    Journal: Journal of Proteome Research

    Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

    doi: 10.1021/pr800904z

    Figure Lengend Snippet: General workflow for processing of serum samples. Pooled serum from pancreatic cancer patients or controls is mixed with SILAC labeled conditioned media from CAPAN-2 cells. Abundant proteins are removed by immunoaffinity depletion. Samples were separated by IEF. IPG strips were then cut into pieces and digested with trypsin. Tryptic peptides were separated by strong cation exchange, then analyzed by reversed-phase LC−MS/MS.

    Article Snippet: As expected, only a negligible amount of protein is lost from SILAP standard samples processed alone using this immunoaffinity column.

    Techniques: Labeling, Electrofocusing, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry