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Agilent technologies immunoaffinity column
Immunoaffinity Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunoaffinity column/product/Agilent technologies
Average 99 stars, based on 1 article reviews
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immunoaffinity column - by Bioz Stars, 2021-03
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Gas Chromatography-Mass Spectrometry:

Article Title: cis-Jasmone Elicits Aphid-Induced Stress Signalling in Potatoes
Article Snippet: HP Chemstation software was used for data analysis. .. Coupled gas chromatography-mass spectrometry (GC-MS) was performed on an Agilent MSD 5972 and Agilent 5890 GC (fitted with a non-polar HP1 column 50 m length × 0.32 mm inner diam. ..

Article Title: Imbalance of gut microbiome and intestinal epithelial barrier dysfunction in patients with high blood pressure
Article Snippet: Samples were dispersed in acidified water spiked with stable-isotope labeled short chain fatty acid standards, and then extracted with diethyl ether. .. The ether layer was immediately analyzed by GC-MS using a Phenomenex ZB-WAX column on an Agilent 6890 GC with a 5973 MS detector. .. Quantitation was performed by calibration to internal butyrate standards.

Article Title: Evaluating PHA Productivity of Bioengineered Rhodosprillum rubrum
Article Snippet: .. The methyl esters were assayed by GC-MS with an Agilent 6890 GC equipped with DB-WAX column (30 m×0.25 mm ID, 0.5 µ m) interfaced to a 5973 mass spectrometer and an electron impact ionization detector (Agilent Technologies, Santa Clara, CA). .. The GC/MS data files were deconvoluted by NIST AMDIS software.

Article Title: Antibiotic-induced shifts in the mouse gut microbiome and metabolome increase susceptibility to Clostridium difficile infection
Article Snippet: Cecal samples were dispersed in acidified water spiked with stable-isotope labeled SCFA standards, and then extracted with diethyl ether. .. The ether layer was immediately analyzed by GC-MS using a Phenomenex ZB-WAX column on an Agilent 6890 GC with a 5973 MS detector. .. The bile acids were detected by negative LC-ESI-triple quadrupole mass spectrometry and 3 of them (LCA, CDCA, and UDCA) were detected by positive mode.

Mass Spectrometry:

Article Title: Imbalance of gut microbiome and intestinal epithelial barrier dysfunction in patients with high blood pressure
Article Snippet: Samples were dispersed in acidified water spiked with stable-isotope labeled short chain fatty acid standards, and then extracted with diethyl ether. .. The ether layer was immediately analyzed by GC-MS using a Phenomenex ZB-WAX column on an Agilent 6890 GC with a 5973 MS detector. .. Quantitation was performed by calibration to internal butyrate standards.

Article Title: Cyanoethylation of the glucans dextran and pullulan: Substitution pattern and formation of nanostructures and entrapment of magnetic nanoparticles
Article Snippet: Peaks were identified by gas chromatography/mass spectrometry (GLC–MS) analysis. .. GLC–MS: Agilent 6890 GC (ZB5-MS column, 30 m, inner diameter 0.25 mm, film thickness 0.25 mm and 1.5 m) and a JEOL GC mate II bench-top double-focusing magnetic sector mass spectrometer. ..

Article Title: Evaluating PHA Productivity of Bioengineered Rhodosprillum rubrum
Article Snippet: .. The methyl esters were assayed by GC-MS with an Agilent 6890 GC equipped with DB-WAX column (30 m×0.25 mm ID, 0.5 µ m) interfaced to a 5973 mass spectrometer and an electron impact ionization detector (Agilent Technologies, Santa Clara, CA). .. The GC/MS data files were deconvoluted by NIST AMDIS software.

Article Title: Antibiotic-induced shifts in the mouse gut microbiome and metabolome increase susceptibility to Clostridium difficile infection
Article Snippet: Cecal samples were dispersed in acidified water spiked with stable-isotope labeled SCFA standards, and then extracted with diethyl ether. .. The ether layer was immediately analyzed by GC-MS using a Phenomenex ZB-WAX column on an Agilent 6890 GC with a 5973 MS detector. .. The bile acids were detected by negative LC-ESI-triple quadrupole mass spectrometry and 3 of them (LCA, CDCA, and UDCA) were detected by positive mode.

Gas Chromatography:

Article Title: Cyanoethylation of the glucans dextran and pullulan: Substitution pattern and formation of nanostructures and entrapment of magnetic nanoparticles
Article Snippet: Peaks were identified by gas chromatography/mass spectrometry (GLC–MS) analysis. .. GLC–MS: Agilent 6890 GC (ZB5-MS column, 30 m, inner diameter 0.25 mm, film thickness 0.25 mm and 1.5 m) and a JEOL GC mate II bench-top double-focusing magnetic sector mass spectrometer. ..

Article Title: Up-regulation of neurotrophic factors by cinnamon and its metabolite sodium benzoate: Therapeutic implications for neurodegenerative disorders
Article Snippet: A JEOL GCMate II (JEOL USA, Peabody MA) gas chromatograph/ mass spectrometer was used in these experiments. .. The gas chromatography column was a fused silica capillary column with a nonpolar 5% phenyl 95% dimethylpolysiloxane phase (Agilent HP-5ms), 30 meters long, 0.25 mm internal diameter, 0.25 um film thickness. .. The carrier gas was Helium (99.9995% Research Grade) run through a STG triple filter (Restek Corp.) at a constant flow rate of 1.1 ml/min.

Article Title: Upregulation of suppressor of cytokine signaling 3 in microglia by cinnamic acid
Article Snippet: A JEOL GCMate II (JEOL USA, Peabody MA) gas chromatograph/mass spectrometer was used for GC-MS as described earlier ( ). .. Briefly, the gas chromatography column was a fused silica capillary column with a nonpolar 5% phenyl 95% dimethylpolysiloxane phase (Agilent HP-5ms), 30 meters long, 0.25 mm internal diameter, 0.25 um film thickness. .. The carrier gas was Helium (99.9995% Research Grade) run through a STG triple filter (Restek Corp.) at a constant flow rate of 1.1 ml/min.

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    Agilent technologies immunoaffinity chromatography
    Electrophoresis separation and purification of individual plasma proteins. Plasma proteins were first fractionated by <t>immunoaffinity</t> chromatography to low abundant and high abundant proteins. Low abundant proteins were separated by 2-dimensional gel electrophoresis
    Immunoaffinity Chromatography, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity chromatography/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity chromatography - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies lmp 1
    <t>LMP-1</t> protein levels in EBV + LCLs are elevated following prolonged growth in IFN-α. 721 cells were treated with IFN-α for 41 days (A) or treated with IFN-α for 14 days, washed, and then cultured in the absence of IFN-α
    Lmp 1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lmp 1/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lmp 1 - by Bioz Stars, 2021-03
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    99
    Agilent technologies immunoaffinity column
    <t>LMP-1</t> protein levels in EBV + LCLs are elevated following prolonged growth in IFN-α. 721 cells were treated with IFN-α for 41 days (A) or treated with IFN-α for 14 days, washed, and then cultured in the absence of IFN-α
    Immunoaffinity Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity column - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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    Electrophoresis separation and purification of individual plasma proteins. Plasma proteins were first fractionated by immunoaffinity chromatography to low abundant and high abundant proteins. Low abundant proteins were separated by 2-dimensional gel electrophoresis

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Differential effects of insulin deprivation and systemic insulin treatment on plasma protein synthesis in type 1 diabetic people

    doi: 10.1152/ajpendo.00351.2009

    Figure Lengend Snippet: Electrophoresis separation and purification of individual plasma proteins. Plasma proteins were first fractionated by immunoaffinity chromatography to low abundant and high abundant proteins. Low abundant proteins were separated by 2-dimensional gel electrophoresis

    Article Snippet: Plasma samples were first fractionated by depleting six high-abundant proteins through immunoaffinity chromatography (Multiple Affinity Removal Column Hu-6; Agilent Technologies).

    Techniques: Electrophoresis, Purification, Chromatography, Nucleic Acid Electrophoresis

    LMP-1 protein levels in EBV + LCLs are elevated following prolonged growth in IFN-α. 721 cells were treated with IFN-α for 41 days (A) or treated with IFN-α for 14 days, washed, and then cultured in the absence of IFN-α

    Journal: Journal of Virology

    Article Title: The Epstein-Barr Virus-Encoded LMP-1 Oncoprotein Negatively Affects Tyk2 Phosphorylation and Interferon Signaling in Human B Cells ▿

    doi: 10.1128/JVI.01570-06

    Figure Lengend Snippet: LMP-1 protein levels in EBV + LCLs are elevated following prolonged growth in IFN-α. 721 cells were treated with IFN-α for 41 days (A) or treated with IFN-α for 14 days, washed, and then cultured in the absence of IFN-α

    Article Snippet: CS1-4 is a pool of four mouse monoclonal antibodies to LMP-1 (Dako).

    Techniques: Cell Culture

    IFN-α-stimulated STAT2 nuclear localization is inhibited in 721 cells expressing high levels of LMP-1. (A, B) LMP-1 and STAT2 staining of 721 cells, untreated (A) or treated with IFN-α for 30 min (B). Cells were fixed, made permeable,

    Journal: Journal of Virology

    Article Title: The Epstein-Barr Virus-Encoded LMP-1 Oncoprotein Negatively Affects Tyk2 Phosphorylation and Interferon Signaling in Human B Cells ▿

    doi: 10.1128/JVI.01570-06

    Figure Lengend Snippet: IFN-α-stimulated STAT2 nuclear localization is inhibited in 721 cells expressing high levels of LMP-1. (A, B) LMP-1 and STAT2 staining of 721 cells, untreated (A) or treated with IFN-α for 30 min (B). Cells were fixed, made permeable,

    Article Snippet: CS1-4 is a pool of four mouse monoclonal antibodies to LMP-1 (Dako).

    Techniques: Expressing, Staining

    LMP-1 negatively regulates Tyk2 and STAT2 phosphorylation. (A) WCLs from DG75 cells transfected with pRSV-LMP-1, pMT2T-Tyk2, or empty vector, analyzed by Western blotting for phosphotyrosine (pY) (top blot), Tyk2 (middle blot), or LMP-1 (lower blot).

    Journal: Journal of Virology

    Article Title: The Epstein-Barr Virus-Encoded LMP-1 Oncoprotein Negatively Affects Tyk2 Phosphorylation and Interferon Signaling in Human B Cells ▿

    doi: 10.1128/JVI.01570-06

    Figure Lengend Snippet: LMP-1 negatively regulates Tyk2 and STAT2 phosphorylation. (A) WCLs from DG75 cells transfected with pRSV-LMP-1, pMT2T-Tyk2, or empty vector, analyzed by Western blotting for phosphotyrosine (pY) (top blot), Tyk2 (middle blot), or LMP-1 (lower blot).

    Article Snippet: CS1-4 is a pool of four mouse monoclonal antibodies to LMP-1 (Dako).

    Techniques: Transfection, Plasmid Preparation, Western Blot

    Tyk2 copurifies with LMP-1. (A) LMP-1 immunoaffinity purification procedure. (B) LMP-1 complexes eluted from anti-LMP-1 antibody affinity column by decreasing pH, analyzed by silver staining (top gel) or Western blotting for LMP-1 and Tyk2 (bottom blots).

    Journal: Journal of Virology

    Article Title: The Epstein-Barr Virus-Encoded LMP-1 Oncoprotein Negatively Affects Tyk2 Phosphorylation and Interferon Signaling in Human B Cells ▿

    doi: 10.1128/JVI.01570-06

    Figure Lengend Snippet: Tyk2 copurifies with LMP-1. (A) LMP-1 immunoaffinity purification procedure. (B) LMP-1 complexes eluted from anti-LMP-1 antibody affinity column by decreasing pH, analyzed by silver staining (top gel) or Western blotting for LMP-1 and Tyk2 (bottom blots).

    Article Snippet: CS1-4 is a pool of four mouse monoclonal antibodies to LMP-1 (Dako).

    Techniques: Immunoaffinity Purification, Affinity Column, Silver Staining, Western Blot

    LMP-1 inhibits IFN-α activation of ISRE elements.

    Journal: Journal of Virology

    Article Title: The Epstein-Barr Virus-Encoded LMP-1 Oncoprotein Negatively Affects Tyk2 Phosphorylation and Interferon Signaling in Human B Cells ▿

    doi: 10.1128/JVI.01570-06

    Figure Lengend Snippet: LMP-1 inhibits IFN-α activation of ISRE elements.

    Article Snippet: CS1-4 is a pool of four mouse monoclonal antibodies to LMP-1 (Dako).

    Techniques: Activation Assay

    LMP-1 inhibits IFN-α-stimulated ISRE activation. DG75 cells were transfected with 6 μg pISRE-Luc (A, B, E) or pGAS-Luc (C, D, F), 1 μg pRSV-LacZ, and the indicated amounts of pRSV-LMP-1 or pRC-RSV vector ( x axis). Two days later,

    Journal: Journal of Virology

    Article Title: The Epstein-Barr Virus-Encoded LMP-1 Oncoprotein Negatively Affects Tyk2 Phosphorylation and Interferon Signaling in Human B Cells ▿

    doi: 10.1128/JVI.01570-06

    Figure Lengend Snippet: LMP-1 inhibits IFN-α-stimulated ISRE activation. DG75 cells were transfected with 6 μg pISRE-Luc (A, B, E) or pGAS-Luc (C, D, F), 1 μg pRSV-LacZ, and the indicated amounts of pRSV-LMP-1 or pRC-RSV vector ( x axis). Two days later,

    Article Snippet: CS1-4 is a pool of four mouse monoclonal antibodies to LMP-1 (Dako).

    Techniques: Activation Assay, Transfection, Plasmid Preparation

    LMP-1 targets Tyk2 and IFN-α signaling in a CTAR-independent manner. (A) LMP-1 mutants. (B) Western analysis of anti-Tyk2 immunoprecipitates from lysates of DG75 cells expressing the indicated LMP-1 mutants (shown below blots). LMP-1 and mutants

    Journal: Journal of Virology

    Article Title: The Epstein-Barr Virus-Encoded LMP-1 Oncoprotein Negatively Affects Tyk2 Phosphorylation and Interferon Signaling in Human B Cells ▿

    doi: 10.1128/JVI.01570-06

    Figure Lengend Snippet: LMP-1 targets Tyk2 and IFN-α signaling in a CTAR-independent manner. (A) LMP-1 mutants. (B) Western analysis of anti-Tyk2 immunoprecipitates from lysates of DG75 cells expressing the indicated LMP-1 mutants (shown below blots). LMP-1 and mutants

    Article Snippet: CS1-4 is a pool of four mouse monoclonal antibodies to LMP-1 (Dako).

    Techniques: Western Blot, Expressing