Journal: iScience

Article Title: OGT/HIF-2α axis promotes the progression of clear cell renal cell carcinoma and regulates its sensitivity to ferroptosis

doi: 10.1016/j.isci.2023.108148

Figure Lengend Snippet: OGT is upregulated in ccRCC and associated with cancer progression (A and B) The protein (A) and mRNA (B) expression of OGT, OGA, and O-GlcNac in ccRCC and paired non-tumor renal tissues (n = 5). (C) The expression of OGT in different grades of ccRCC (Grade 1: n = 36, Grade 2: n = 42, Grade 3: n = 10, Grade 4: n = 4) detected by IHC staining. (D) The expression of OGT in different pathological stages of ccRCC (Stage Ⅰ: n = 72, Stage Ⅱ: n = 6, Stage Ⅲ: n = 14) detected by IHC staining. (E) The association between OGT and overall survival of 512 cases patients with ccRCC in the TCGA database (Kaplan-Meier analysis). (F–H) The association between OGT (F), OGA (G), O-GlcNac (H) and overall survival of our 92 cases of patients with ccRCC (Kaplan-Meier analysis). Scale bar, 400 μm. Data are represented as mean ± SD. Statistical analysis of the data from 2 groups was performed using Student’s t test. Comparisons among multiple groups were performed by one-way ANOVA followed by Fisher’s LSD test. (∗p < 0.05, ∗∗∗p < 0.001).

Article Snippet: Then, the lysates were incubated overnight with O-GlcNAc [GlcNAc-S/T] Immunoaffinity Beads (Cell Signaling Technology, Inc. #95220) at 4°C.

Techniques: Expressing, Immunohistochemistry

Journal: iScience

Article Title: OGT/HIF-2α axis promotes the progression of clear cell renal cell carcinoma and regulates its sensitivity to ferroptosis

doi: 10.1016/j.isci.2023.108148

Figure Lengend Snippet: The association between OGT, OGA, O-GlcNac and clinical features in patients with ccRCC

Article Snippet: Then, the lysates were incubated overnight with O-GlcNAc [GlcNAc-S/T] Immunoaffinity Beads (Cell Signaling Technology, Inc. #95220) at 4°C.

Techniques:

Journal: iScience

Article Title: OGT/HIF-2α axis promotes the progression of clear cell renal cell carcinoma and regulates its sensitivity to ferroptosis

doi: 10.1016/j.isci.2023.108148

Figure Lengend Snippet:

Article Snippet: Then, the lysates were incubated overnight with O-GlcNAc [GlcNAc-S/T] Immunoaffinity Beads (Cell Signaling Technology, Inc. #95220) at 4°C.

Techniques: Recombinant, Luciferase, CRISPR, Plasmid Preparation, Software