immunoaffinity beads conjugated to succinyllysine antibody  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc immunoaffinity beads conjugated to succinyllysine antibody
    Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative <t>anti-succinyllysine</t> Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).
    Immunoaffinity Beads Conjugated To Succinyllysine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity beads conjugated to succinyllysine antibody/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity beads conjugated to succinyllysine antibody - by Bioz Stars, 2023-12
    95/100 stars

    Images

    1) Product Images from "Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism"

    Article Title: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism

    Journal: Journal of proteome research

    doi: 10.1021/acs.jproteome.8b00800

    Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).
    Figure Legend Snippet: Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).

    Techniques Used: Western Blot

    Ethanol metabolism alters hepatic protein acylation and lipid deposition in WT, SIRT3 KO, and SIRT5 KO mice. Paraffin embedded formalin fixed hepatic tissue sections were analyzed using (A) hematoxylin and eosin (H&E) staining and IHC for (B) Plin2, (C) acetyllysine, and (D) succinyllysine. [CV = central vein, PT= portal vein. (10× mag), (n=3)] See also Figures S1–S3.
    Figure Legend Snippet: Ethanol metabolism alters hepatic protein acylation and lipid deposition in WT, SIRT3 KO, and SIRT5 KO mice. Paraffin embedded formalin fixed hepatic tissue sections were analyzed using (A) hematoxylin and eosin (H&E) staining and IHC for (B) Plin2, (C) acetyllysine, and (D) succinyllysine. [CV = central vein, PT= portal vein. (10× mag), (n=3)] See also Figures S1–S3.

    Techniques Used: Staining

    immunoaffinity beads conjugated to succinyllysine antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc immunoaffinity beads conjugated to succinyllysine antibody
    Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative <t>anti-succinyllysine</t> Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).
    Immunoaffinity Beads Conjugated To Succinyllysine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity beads conjugated to succinyllysine antibody/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity beads conjugated to succinyllysine antibody - by Bioz Stars, 2023-12
    95/100 stars

    Images

    1) Product Images from "Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism"

    Article Title: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism

    Journal: Journal of proteome research

    doi: 10.1021/acs.jproteome.8b00800

    Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).
    Figure Legend Snippet: Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).

    Techniques Used: Western Blot

    Ethanol metabolism alters hepatic protein acylation and lipid deposition in WT, SIRT3 KO, and SIRT5 KO mice. Paraffin embedded formalin fixed hepatic tissue sections were analyzed using (A) hematoxylin and eosin (H&E) staining and IHC for (B) Plin2, (C) acetyllysine, and (D) succinyllysine. [CV = central vein, PT= portal vein. (10× mag), (n=3)] See also Figures S1–S3.
    Figure Legend Snippet: Ethanol metabolism alters hepatic protein acylation and lipid deposition in WT, SIRT3 KO, and SIRT5 KO mice. Paraffin embedded formalin fixed hepatic tissue sections were analyzed using (A) hematoxylin and eosin (H&E) staining and IHC for (B) Plin2, (C) acetyllysine, and (D) succinyllysine. [CV = central vein, PT= portal vein. (10× mag), (n=3)] See also Figures S1–S3.

    Techniques Used: Staining

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    • immunoaffinity beads conjugated to succinyllysine antibody  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc immunoaffinity beads conjugated to succinyllysine antibody
    Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative <t>anti-succinyllysine</t> Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).
    Immunoaffinity Beads Conjugated To Succinyllysine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity beads conjugated to succinyllysine antibody/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoaffinity beads conjugated to succinyllysine antibody - by Bioz Stars, 2023-12
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).

    Journal: Journal of proteome research

    Article Title: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism

    doi: 10.1021/acs.jproteome.8b00800

    Figure Lengend Snippet: Ethanol increases lysine acetylation and decreases succinylation of mitochondrial proteins in WT, SIRT3 KO, and SIRT5 KO mouse livers. (A) Representative anti-acetyllysine Western blot. (B) Representative anti-succinyllysine Western blot. (C) Representative SIRT3, SIRT5, and loading control (COXIV) Western blots. (D-G) Corresponding densitometry quantification using unpaired Student’s t-test (mean ± SEM, n=3, *=p<0.05, **=p<0.005, ***=p<0.0005, ****=p<0.0001).

    Article Snippet: Samples were incubated 2 hours at 4 °C with immunoaffinity beads conjugated to acetyllysine antibody or immunoaffinity beads conjugated to succinyllysine antibody (PTMScan® Acetyl-Lysine Motif [Ac-K] Immunoaffinity Beads #13416 and PTMScan® Succinyl-Lysine Motif [Succ-K] Immunoaffinity Beads #13764, Cell Signaling, Boston, MA).

    Techniques: Western Blot

    Ethanol metabolism alters hepatic protein acylation and lipid deposition in WT, SIRT3 KO, and SIRT5 KO mice. Paraffin embedded formalin fixed hepatic tissue sections were analyzed using (A) hematoxylin and eosin (H&E) staining and IHC for (B) Plin2, (C) acetyllysine, and (D) succinyllysine. [CV = central vein, PT= portal vein. (10× mag), (n=3)] See also Figures S1–S3.

    Journal: Journal of proteome research

    Article Title: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism

    doi: 10.1021/acs.jproteome.8b00800

    Figure Lengend Snippet: Ethanol metabolism alters hepatic protein acylation and lipid deposition in WT, SIRT3 KO, and SIRT5 KO mice. Paraffin embedded formalin fixed hepatic tissue sections were analyzed using (A) hematoxylin and eosin (H&E) staining and IHC for (B) Plin2, (C) acetyllysine, and (D) succinyllysine. [CV = central vein, PT= portal vein. (10× mag), (n=3)] See also Figures S1–S3.

    Article Snippet: Samples were incubated 2 hours at 4 °C with immunoaffinity beads conjugated to acetyllysine antibody or immunoaffinity beads conjugated to succinyllysine antibody (PTMScan® Acetyl-Lysine Motif [Ac-K] Immunoaffinity Beads #13416 and PTMScan® Succinyl-Lysine Motif [Succ-K] Immunoaffinity Beads #13764, Cell Signaling, Boston, MA).

    Techniques: Staining