immulon 2hb 96 well plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher immulon 2hb 96 well plates
    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of <t>Immulon</t> 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.
    Immulon 2hb 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immulon 2hb 96 well plates/product/Thermo Fisher
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    immulon 2hb 96 well plates - by Bioz Stars, 2020-04
    94/100 stars

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    1) Product Images from "Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *"

    Article Title: Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.353144

    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of Immulon 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.
    Figure Legend Snippet: Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of Immulon 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.

    Techniques Used: Binding Assay, Recombinant, Incubation, Sequencing

    Binding of PC12 cells to Toolkits II and III and selected peptides. Immulon 2HB 96-well plates or E-Plates TM for the xCELLigence instrument were coated with the indicated peptides as described under “Experimental Procedures.” a and b , PC12 cells were seeded at 10 5 /well, and SPBA was performed after 1 h as described using the colorimetric lactate dehydrogenase assay kit. c and d , PC12 cells were seeded onto E-Plates at 2.5 × 10 4 cells/well, and the cell index was measured continually. PC12 cell binding to Toolkits II ( a ) and III ( b ) is shown. PC12 cell adhesion to the indicated Toolkit peptides ( c ) and to selected shorter integrin-binding peptides ( d ) is shown as end point data after 2 h of adhesion. All experiments were performed in triplicate in the presence of either 2 m m Mg 2+ or EDTA as indicated. Data are the mean ± S.E. of three independent experiments.
    Figure Legend Snippet: Binding of PC12 cells to Toolkits II and III and selected peptides. Immulon 2HB 96-well plates or E-Plates TM for the xCELLigence instrument were coated with the indicated peptides as described under “Experimental Procedures.” a and b , PC12 cells were seeded at 10 5 /well, and SPBA was performed after 1 h as described using the colorimetric lactate dehydrogenase assay kit. c and d , PC12 cells were seeded onto E-Plates at 2.5 × 10 4 cells/well, and the cell index was measured continually. PC12 cell binding to Toolkits II ( a ) and III ( b ) is shown. PC12 cell adhesion to the indicated Toolkit peptides ( c ) and to selected shorter integrin-binding peptides ( d ) is shown as end point data after 2 h of adhesion. All experiments were performed in triplicate in the presence of either 2 m m Mg 2+ or EDTA as indicated. Data are the mean ± S.E. of three independent experiments.

    Techniques Used: Binding Assay, Lactate Dehydrogenase Assay

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Antigen Is Required for Maturation and Activation of Pathogenic Anti-DNA Antibodies and Systemic Inflammation
    Article Snippet: Paragraph title: Anti-dsDNA Ab ELISA ... Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA that had been passed through a nitro-cellulose filter to remove ssDNA.

    Article Title: Immunotherapy to improve cognition and reduce pathological species in an Alzheimer’s disease mouse model
    Article Snippet: Paragraph title: ELISA ... For direct ELISA experiments to determine the binding affinity (KD ) value, Immulon 2HB 96-well plates (Thermo, Waltham, MA) were coated per well with 0.2 μg Aβ oligomers, prepared as previously published [ ] in 50 mM ammonium bicarbonate solution, pH 9.6, overnight at 4 °C.

    Article Title: Recombinant human VEGF165b protein is an effective anti-cancer agent in mice
    Article Snippet: Paragraph title: 2.6. ELISA for VEGF 165 b or VEGF 165 ​ in plasma ... Briefly, immulon 2HB 96-well plates (Thermo Scientific, Basingstoke, UK) were coated with 0.8 μg/ml goat anti-hVEGF capture antibody and blocked with 5% w/v BSA followed by the addition of samples.

    Article Title: Clearance of Pneumococcal Colonization in Infants Is Delayed through Altered Macrophage Trafficking
    Article Snippet: .. ELISA For measurements of anti-pneumococcal antibody titers, pneumococcal strain P1121 was grown and resuspended to an OD620 of 0.1 in coating buffer (0.015 M Na2 CO3 , 0.035 M NaHCO3 ), then plated onto Immulon 2HB 96-well plates (Thermo) at 4°C overnight. ..

    Article Title: Soluble T Cell Immunoglobulin Mucin Domain 3 Is Shed from CD8+ T Cells by the Sheddase ADAM10, Is Increased in Plasma during Untreated HIV Infection, and Correlates with HIV Disease Progression
    Article Snippet: Paragraph title: Soluble Tim-3 ELISA. ... To measure levels of sTim-3 in human plasma and in vitro culture supernatant samples, Immulon 2HB 96-well plates (Thermo Scientific, Rockford, IL) were coated overnight at 4°C with an unconjugated anti-Tim-3 MAb (clone 344823; R & D Systems) at 2.1 μg/ml in PBS.

    Binding Assay:

    Article Title: Immunotherapy to improve cognition and reduce pathological species in an Alzheimer’s disease mouse model
    Article Snippet: .. For direct ELISA experiments to determine the binding affinity (KD ) value, Immulon 2HB 96-well plates (Thermo, Waltham, MA) were coated per well with 0.2 μg Aβ oligomers, prepared as previously published [ ] in 50 mM ammonium bicarbonate solution, pH 9.6, overnight at 4 °C. .. Blocking, washes, and antibody incubations were followed as described above.

    Article Title: Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *
    Article Snippet: Paragraph title: I Domain Binding Assay ... Peptides were coated at 10 μg/ml for 1 h at 22 °C on Immulon 2HB 96-well plates (Thermo Scientific) and blocked for 1 h with 200 μl of TBS containing 50 mg/ml BSA.

    In Vitro:

    Article Title: Soluble T Cell Immunoglobulin Mucin Domain 3 Is Shed from CD8+ T Cells by the Sheddase ADAM10, Is Increased in Plasma during Untreated HIV Infection, and Correlates with HIV Disease Progression
    Article Snippet: .. To measure levels of sTim-3 in human plasma and in vitro culture supernatant samples, Immulon 2HB 96-well plates (Thermo Scientific, Rockford, IL) were coated overnight at 4°C with an unconjugated anti-Tim-3 MAb (clone 344823; R & D Systems) at 2.1 μg/ml in PBS. ..

    Serial Dilution:

    Article Title: Hormonal milieu at time of B cell activation controls duration of autoantibody response
    Article Snippet: Briefly, Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA. .. Splenocytes from P- or hormone-treated R4Atg mice were added to the plates in serial dilution and incubated for 12 h at 37 °C followed by biotin-conjugated anti-mouse IgG (1:600 dilution, Southern Biotechnology) and alkaline phosphatase (AP)-conjugated streptavidin (Southern Biotechnology).

    Cell Culture:

    Article Title: Memory B cells from a subset of treatment na?ve relapsing remitting multiple sclerosis patients elicit CD4+ T cell proliferation and IFN-? production in response to MBP and MOG
    Article Snippet: Paragraph title: MEASUREMENT OF CYTOKINE SECRETION IN B-T CELL CULTURE SUPERNATANTS ... Interleukin-5 (IL-5), Interferon-gamma (IFN-γ), Interleukin-10 (IL-10), or lymphotoxin-alpha (LTα) capture monoclonal antibodies were diluted to 2 μg/mL in 0.1 M NaHCO3 and coated onto Immulon 2HB 96-well plates (Thermo-Fisher Scientific, Pittsburgh, PA) overnight at 4°C.

    Incubation:

    Article Title: Immunotherapy to improve cognition and reduce pathological species in an Alzheimer’s disease mouse model
    Article Snippet: After washing, 50 μl of goat anti-mouse IgG horseradish peroxidase (1:5000; GE Healthcare, UK) was added and incubated for 1 h. Plates were washed again, and 100 μl of tetramethyl benzidine substrate solution (TMB; Thermo Scientific, Rockford, IL) was added and incubated. .. For direct ELISA experiments to determine the binding affinity (KD ) value, Immulon 2HB 96-well plates (Thermo, Waltham, MA) were coated per well with 0.2 μg Aβ oligomers, prepared as previously published [ ] in 50 mM ammonium bicarbonate solution, pH 9.6, overnight at 4 °C.

    Article Title: Recombinant human VEGF165b protein is an effective anti-cancer agent in mice
    Article Snippet: Briefly, immulon 2HB 96-well plates (Thermo Scientific, Basingstoke, UK) were coated with 0.8 μg/ml goat anti-hVEGF capture antibody and blocked with 5% w/v BSA followed by the addition of samples. .. Detection was done by the addition of biotinylated mouse monoclonal anti-VEGF165 b antibody at 0.4 μg/ml followed by incubation with horseradish peroxidase-conjugated streptavidin and substrate development before being read at 450 nm and 570 nm for reference.

    Article Title: Memory B cells from a subset of treatment na?ve relapsing remitting multiple sclerosis patients elicit CD4+ T cell proliferation and IFN-? production in response to MBP and MOG
    Article Snippet: Interleukin-5 (IL-5), Interferon-gamma (IFN-γ), Interleukin-10 (IL-10), or lymphotoxin-alpha (LTα) capture monoclonal antibodies were diluted to 2 μg/mL in 0.1 M NaHCO3 and coated onto Immulon 2HB 96-well plates (Thermo-Fisher Scientific, Pittsburgh, PA) overnight at 4°C. .. Plates were then washed twice with wash buffer (PBS, 0.1% v/v Tween-20) and incubated with blocking buffer (1% BSA in PBS) for 2 hours at room temperature.

    Article Title: Inhibition of Glycoprotein VI Clustering by Collagen as a Mechanism of Inhibiting Collagen-Induced Platelet Responses: The Example of Losartan
    Article Snippet: Briefly, Immulon 2HB 96-well plates (Thermo Scientific, MA, USA) were coated with Horm collagen (100 μL, 10 μg.mL-1 ) or GFOGER (100 μL, 20 μg.mL-1 ) overnight at 4°C. .. After saturation with 10 mg.mL-1 BSA in PBS for 2 hours at RT, washed platelets (2×108 .mL-1 ) in reaction buffer completed or not with Mg2+ , treated or not with 22 μM losartan, were incubated into collagen- or GFOGER-coated wells for different times.

    Article Title: Hormonal milieu at time of B cell activation controls duration of autoantibody response
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    Article Title: Nod2 sensing of lysozyme-digested peptidoglycan promotes macrophage recruitment and clearance of S. pneumoniae colonization in mice
    Article Snippet: .. Cells were fixed by overnight incubation at 4°C on Immulon 2HB 96-well plates (Thermo). .. Plates were blocked using 1% BSA (Sigma-Aldrich) in PBS and washed between steps with PBS containing Brij 35 (0.05%).

    Microscopy:

    Article Title: Hormonal milieu at time of B cell activation controls duration of autoantibody response
    Article Snippet: Briefly, Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA. .. The plates were developed with 5-bromo-4-chloro-3-indolyl phosphate substrate (Sigma Aldrich) and spots were counted under a dissecting microscope.

    Mouse Assay:

    Article Title: Hormonal milieu at time of B cell activation controls duration of autoantibody response
    Article Snippet: Briefly, Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA. .. Splenocytes from P- or hormone-treated R4Atg mice were added to the plates in serial dilution and incubated for 12 h at 37 °C followed by biotin-conjugated anti-mouse IgG (1:600 dilution, Southern Biotechnology) and alkaline phosphatase (AP)-conjugated streptavidin (Southern Biotechnology).

    Produced:

    Article Title: Selecting the correct cellular model for assessing of the biological response of collagen-based biomaterials
    Article Snippet: .. Collagens and peptides coatings were produced on the surface of Immulon 2HB 96-well plates (Thermo Scientific) by incubating 100 µl/well of 10 µg/ml solution or suspension (for insoluble collagen type I) in 10 mM acetic acid containing the appropriate proteins/peptides over night at 4 °C. .. Bovine serum albumin (BSA, Sigma) and triple-helical-like sequences GPP10 were plated in triplicate to act as nonspecific background adhesion controls.

    Article Title: Selecting the correct cellular model for assessing of the biological response of collagen-based biomaterials
    Article Snippet: .. 2.2.1 Monolayer coated surfaces Collagens and peptides coatings were produced on the surface of Immulon 2HB 96-well plates (Thermo Scientific) by incubating 100 µl/well of 10 µg/ml solution or suspension (for insoluble collagen type I) in 10 mM acetic acid containing the appropriate proteins/peptides over night at 4 °C. .. Bovine serum albumin (BSA, Sigma) and triple-helical-like sequences GPP10 were plated in triplicate to act as nonspecific background adhesion controls.

    Concentration Assay:

    Article Title: Antigen Is Required for Maturation and Activation of Pathogenic Anti-DNA Antibodies and Systemic Inflammation
    Article Snippet: Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA that had been passed through a nitro-cellulose filter to remove ssDNA. .. Purified R4A mAb was used (1–50 μg/ml) to generate a standard curve for calculating the concentration of anti-dsDNA Abs in the sera.

    Article Title: Immunotherapy to improve cognition and reduce pathological species in an Alzheimer’s disease mouse model
    Article Snippet: The relationship between OD and antibody concentration was determined by a four-parameter logistic log function. .. For direct ELISA experiments to determine the binding affinity (KD ) value, Immulon 2HB 96-well plates (Thermo, Waltham, MA) were coated per well with 0.2 μg Aβ oligomers, prepared as previously published [ ] in 50 mM ammonium bicarbonate solution, pH 9.6, overnight at 4 °C.

    Purification:

    Article Title: Antigen Is Required for Maturation and Activation of Pathogenic Anti-DNA Antibodies and Systemic Inflammation
    Article Snippet: Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA that had been passed through a nitro-cellulose filter to remove ssDNA. .. Purified R4A mAb was used (1–50 μg/ml) to generate a standard curve for calculating the concentration of anti-dsDNA Abs in the sera.

    Direct ELISA:

    Article Title: Immunotherapy to improve cognition and reduce pathological species in an Alzheimer’s disease mouse model
    Article Snippet: .. For direct ELISA experiments to determine the binding affinity (KD ) value, Immulon 2HB 96-well plates (Thermo, Waltham, MA) were coated per well with 0.2 μg Aβ oligomers, prepared as previously published [ ] in 50 mM ammonium bicarbonate solution, pH 9.6, overnight at 4 °C. .. Blocking, washes, and antibody incubations were followed as described above.

    Activity Assay:

    Article Title: Inhibition of Glycoprotein VI Clustering by Collagen as a Mechanism of Inhibiting Collagen-Induced Platelet Responses: The Example of Losartan
    Article Snippet: Briefly, Immulon 2HB 96-well plates (Thermo Scientific, MA, USA) were coated with Horm collagen (100 μL, 10 μg.mL-1 ) or GFOGER (100 μL, 20 μg.mL-1 ) overnight at 4°C. .. After washing, the amount of adherent platelets was determined by quantifying alkaline phosphatase activity using p-nitrophenyl phosphate (pNPP).

    Blocking Assay:

    Article Title: Antigen Is Required for Maturation and Activation of Pathogenic Anti-DNA Antibodies and Systemic Inflammation
    Article Snippet: Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA that had been passed through a nitro-cellulose filter to remove ssDNA. .. Mouse serum at different dilutions was added to plates after blocking with 1.0% BSA/PBS.

    Article Title: Immunotherapy to improve cognition and reduce pathological species in an Alzheimer’s disease mouse model
    Article Snippet: For direct ELISA experiments to determine the binding affinity (KD ) value, Immulon 2HB 96-well plates (Thermo, Waltham, MA) were coated per well with 0.2 μg Aβ oligomers, prepared as previously published [ ] in 50 mM ammonium bicarbonate solution, pH 9.6, overnight at 4 °C. .. Blocking, washes, and antibody incubations were followed as described above.

    Article Title: Memory B cells from a subset of treatment na?ve relapsing remitting multiple sclerosis patients elicit CD4+ T cell proliferation and IFN-? production in response to MBP and MOG
    Article Snippet: Interleukin-5 (IL-5), Interferon-gamma (IFN-γ), Interleukin-10 (IL-10), or lymphotoxin-alpha (LTα) capture monoclonal antibodies were diluted to 2 μg/mL in 0.1 M NaHCO3 and coated onto Immulon 2HB 96-well plates (Thermo-Fisher Scientific, Pittsburgh, PA) overnight at 4°C. .. Plates were then washed twice with wash buffer (PBS, 0.1% v/v Tween-20) and incubated with blocking buffer (1% BSA in PBS) for 2 hours at room temperature.

    Article Title: Soluble T Cell Immunoglobulin Mucin Domain 3 Is Shed from CD8+ T Cells by the Sheddase ADAM10, Is Increased in Plasma during Untreated HIV Infection, and Correlates with HIV Disease Progression
    Article Snippet: To measure levels of sTim-3 in human plasma and in vitro culture supernatant samples, Immulon 2HB 96-well plates (Thermo Scientific, Rockford, IL) were coated overnight at 4°C with an unconjugated anti-Tim-3 MAb (clone 344823; R & D Systems) at 2.1 μg/ml in PBS. .. Each sample was diluted 1-in-2 with blocking buffer (3% bovine serum albumin [BSA] in PBS-Tween 20 [PBST]).

    Sonication:

    Article Title: Antigen Is Required for Maturation and Activation of Pathogenic Anti-DNA Antibodies and Systemic Inflammation
    Article Snippet: .. Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA that had been passed through a nitro-cellulose filter to remove ssDNA. .. Mouse serum at different dilutions was added to plates after blocking with 1.0% BSA/PBS.

    Article Title: Hormonal milieu at time of B cell activation controls duration of autoantibody response
    Article Snippet: .. Briefly, Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA. .. Splenocytes from P- or hormone-treated R4Atg mice were added to the plates in serial dilution and incubated for 12 h at 37 °C followed by biotin-conjugated anti-mouse IgG (1:600 dilution, Southern Biotechnology) and alkaline phosphatase (AP)-conjugated streptavidin (Southern Biotechnology).

    Activated Clotting Time Assay:

    Article Title: Selecting the correct cellular model for assessing of the biological response of collagen-based biomaterials
    Article Snippet: Collagens and peptides coatings were produced on the surface of Immulon 2HB 96-well plates (Thermo Scientific) by incubating 100 µl/well of 10 µg/ml solution or suspension (for insoluble collagen type I) in 10 mM acetic acid containing the appropriate proteins/peptides over night at 4 °C. .. Bovine serum albumin (BSA, Sigma) and triple-helical-like sequences GPP10 were plated in triplicate to act as nonspecific background adhesion controls.

    Article Title: Selecting the correct cellular model for assessing of the biological response of collagen-based biomaterials
    Article Snippet: 2.2.1 Monolayer coated surfaces Collagens and peptides coatings were produced on the surface of Immulon 2HB 96-well plates (Thermo Scientific) by incubating 100 µl/well of 10 µg/ml solution or suspension (for insoluble collagen type I) in 10 mM acetic acid containing the appropriate proteins/peptides over night at 4 °C. .. Bovine serum albumin (BSA, Sigma) and triple-helical-like sequences GPP10 were plated in triplicate to act as nonspecific background adhesion controls.

    Recombinant:

    Article Title: Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *
    Article Snippet: Peptides were coated at 10 μg/ml for 1 h at 22 °C on Immulon 2HB 96-well plates (Thermo Scientific) and blocked for 1 h with 200 μl of TBS containing 50 mg/ml BSA. .. Wells were washed four times with 200 μl of adhesion buffer (TBS with 1 mg/ml BSA) before the addition of 100 μl of adhesion buffer containing 1 μg/ml recombinant GST-I domains in the presence of either 2 mm MgCl2 or EDTA for 1 h at room temperature.

    Enzyme-linked Immunospot:

    Article Title: Hormonal milieu at time of B cell activation controls duration of autoantibody response
    Article Snippet: Paragraph title: 2.6. ELISpot assay ... Briefly, Immulon 2HB 96-well plates (Thermo LabSystems) were coated with 100 μl/ml sonicated calf thymus DNA.

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    Thermo Fisher immulon 2hb 96 well plates
    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of <t>Immulon</t> 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.
    Immulon 2hb 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immulon 2hb 96 well plates/product/Thermo Fisher
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    immulon 2hb 96 well plates - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    92
    Thermo Fisher elisa immulon 96 well plates
    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of <t>Immulon</t> 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.
    Elisa Immulon 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa immulon 96 well plates/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa immulon 96 well plates - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    85
    Thermo Fisher immulon 2hb
    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of <t>Immulon</t> 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.
    Immulon 2hb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immulon 2hb/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immulon 2hb - by Bioz Stars, 2020-04
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    Image Search Results


    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of Immulon 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.

    Journal: The Journal of Biological Chemistry

    Article Title: Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *

    doi: 10.1074/jbc.M112.353144

    Figure Lengend Snippet: Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of Immulon 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.

    Article Snippet: Peptides were coated at 10 μg/ml for 1 h at 22 °C on Immulon 2HB 96-well plates (Thermo Scientific) and blocked for 1 h with 200 μl of TBS containing 50 mg/ml BSA.

    Techniques: Binding Assay, Recombinant, Incubation, Sequencing

    Binding of PC12 cells to Toolkits II and III and selected peptides. Immulon 2HB 96-well plates or E-Plates TM for the xCELLigence instrument were coated with the indicated peptides as described under “Experimental Procedures.” a and b , PC12 cells were seeded at 10 5 /well, and SPBA was performed after 1 h as described using the colorimetric lactate dehydrogenase assay kit. c and d , PC12 cells were seeded onto E-Plates at 2.5 × 10 4 cells/well, and the cell index was measured continually. PC12 cell binding to Toolkits II ( a ) and III ( b ) is shown. PC12 cell adhesion to the indicated Toolkit peptides ( c ) and to selected shorter integrin-binding peptides ( d ) is shown as end point data after 2 h of adhesion. All experiments were performed in triplicate in the presence of either 2 m m Mg 2+ or EDTA as indicated. Data are the mean ± S.E. of three independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *

    doi: 10.1074/jbc.M112.353144

    Figure Lengend Snippet: Binding of PC12 cells to Toolkits II and III and selected peptides. Immulon 2HB 96-well plates or E-Plates TM for the xCELLigence instrument were coated with the indicated peptides as described under “Experimental Procedures.” a and b , PC12 cells were seeded at 10 5 /well, and SPBA was performed after 1 h as described using the colorimetric lactate dehydrogenase assay kit. c and d , PC12 cells were seeded onto E-Plates at 2.5 × 10 4 cells/well, and the cell index was measured continually. PC12 cell binding to Toolkits II ( a ) and III ( b ) is shown. PC12 cell adhesion to the indicated Toolkit peptides ( c ) and to selected shorter integrin-binding peptides ( d ) is shown as end point data after 2 h of adhesion. All experiments were performed in triplicate in the presence of either 2 m m Mg 2+ or EDTA as indicated. Data are the mean ± S.E. of three independent experiments.

    Article Snippet: Peptides were coated at 10 μg/ml for 1 h at 22 °C on Immulon 2HB 96-well plates (Thermo Scientific) and blocked for 1 h with 200 μl of TBS containing 50 mg/ml BSA.

    Techniques: Binding Assay, Lactate Dehydrogenase Assay