Journal: Neural Regeneration Research

Article Title: Chitosan conduits enriched with fibrin-collagen hydrogel with or without adipose-derived mesenchymal stem cells for the repair of 15-mm-long sciatic nerve defect

doi: 10.4103/1673-5374.358605

Figure Lengend Snippet: Primary and secondary antibodies used in western blot analysis

Article Snippet: ImmPRESS® HRP anti-mouse IgG (Peroxidase) , Ready to use , MP-7402 , AB_2336528 , 30 min at RT , – , Vector Laboratories, Inc. Burlingame, CA, USA.

Techniques: Western Blot

Journal: Neural Regeneration Research

Article Title: Chitosan conduits enriched with fibrin-collagen hydrogel with or without adipose-derived mesenchymal stem cells for the repair of 15-mm-long sciatic nerve defect

doi: 10.4103/1673-5374.358605

Figure Lengend Snippet: Antibodies/reagents used in immunohistochemical analysis

Article Snippet: ImmPRESS® HRP anti-mouse IgG (Peroxidase) , Ready to use , MP-7402 , AB_2336528 , 30 min at RT , – , Vector Laboratories, Inc. Burlingame, CA, USA.

Techniques: Immunohistochemical staining, Incubation, Plasmid Preparation

Journal: Frontiers in Neuroanatomy

Article Title: The olfactory limbus of the red fox ( Vulpes vulpes ). New insights regarding a noncanonical olfactory bulb pathway

doi: 10.3389/fnana.2022.1097467

Figure Lengend Snippet: Detailed information on the antibodies and lectins used in this study.

Article Snippet: To prevent non-specific binding sites, 2.5% horse normal serum from the ImmPRESS reagent kit Anti-mouse IgG/Anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) was used for 30 min ( ).

Techniques: Binding Assay, Recombinant, Plasmid Preparation

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet:

Article Snippet: Substituting Opal™ Polymer HRP Ms + Rb secondary antibody with ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (Vector Laboratories, Cat# MP-7500 ) should substantially resolve any low intensity signals.

Techniques: Plasmid Preparation, Recombinant, Software, Staining, Imaging

Journal: Theranostics

Article Title: Experimental pancreatic cancer develops in soft pancreas: novel leads for an individualized diagnosis by ultrafast elasticity imaging

doi: 10.7150/thno.34066

Figure Lengend Snippet: Markers of senescence are present in the pancreatic pretumoral niche, where increased elasticity was measured. A-B: H&E, IHC using indicated antibodies & picrosirius red analysis of pancreas (Ctrl, pretumoral or KPC at euthanasia). Scale: 200µm. Red arrows represent senescent cells. Black arrows represent positive DAB (p16) or AEC (F4/80) or picro sirius red stainings. C-E: Quantification of stainings in B (mean area per field in ≥ 3 images at 63 x) are presented as mean ± SEM. n=3 in each group. Mann Whitney test: *: p<0.05; **: p<0.01; ***: p<0.001. F-H: Circulating granulocyte, neutrophil, platelet values represented as ratios to circulating lymphocytes. Mean± SEM. n≥3 in each group Mann Whitney test: *: p<0.05; **: p<0.01; ***: p<0.001.

Article Snippet: Antigen retrieval and antibody dilution (p16, F12 clone, Sigma #sc-1661, 1/100, citrate antigen retrieval; F4/80, Cl A3-1 clone, Pierce #MA1-91124, 1/100, Proteinase K antigen retrieval) was carried out as described in table below followed by AEC or DAB incubation prior secondary antibody (ImmPress (MP-7402, Vector) or BA-4001 (1/50, Vector)).

Techniques: MANN-WHITNEY