immpact dab peroxidase hrp substrate  (Vector Laboratories)


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    ImmPACT DAB Peroxidase HRP Substrate
    Description:
    ImmPACT DAB Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsHeat StablePermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated DAB stock solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This product is an entirely new formula for a diaminobenzidine based peroxidase substrate ImmPACT DAB produces a dark brown reaction product that is crisper and is 3 4 times more sensitive than the original Vector DAB substrate kit It can be used for both manual and automated staining methods The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB This product can also be used on blots
    Catalog Number:
    SK-4105
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Size:
    120 ml
    Buy from Supplier


    Structured Review

    Vector Laboratories immpact dab peroxidase hrp substrate
    ImmPACT DAB Peroxidase HRP Substrate
    ImmPACT DAB Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsHeat StablePermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated DAB stock solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This product is an entirely new formula for a diaminobenzidine based peroxidase substrate ImmPACT DAB produces a dark brown reaction product that is crisper and is 3 4 times more sensitive than the original Vector DAB substrate kit It can be used for both manual and automated staining methods The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB This product can also be used on blots
    https://www.bioz.com/result/immpact dab peroxidase hrp substrate/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpact dab peroxidase hrp substrate - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: Then the slices were incubated with the second biotinylated antibodies (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) for 30 min. .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Article Title: Inositol 1,4,5-triphosphate receptor is selectively expressed in cerebellum but not cerebellum-like structures of the elasmobranch fish, Leucoraja erinacea
    Article Snippet: Sections were incubated in primary antibody for 12–15 hours at room temperature followed by biotinylated secondary antibody (Vector Laboratories goatαrabbit Catalog # PK-6101 ) in blocking buffer for 30 minutes at room temperature. .. Slides were incubated in ABC reagent for 30 minutes, then in a DAB peroxidase substrate (Vector Laboratories ImmPACT DAB peroxidase (HRP) substrate Catalog # SK-4105 ) for two minutes and dehydrated in an ethyl alcohol series and cleared in xylene. .. A no-primary negative control was done for each protein.

    Article Title: The absence of the leukotriene B4 receptor BLT1 attenuates peripheral inflammation and spinal nociceptive processing following intraplantar formalin injury
    Article Snippet: Sections were then incubated with a rabbit p-S133 rabbit monoclonal antibody for pCREB (1:400, Cell Signaling Technology, Danvers, MA, USA) containing 0.25% normal goat serum overnight at 4°C. .. The sections were incubated with biotinylated goat anti-rabbit IgG (1:200, Vector Laboratories, Burlingame, CA, USA) at room temperature for 1 h and then incubated in Vectastain Elite ABC Reagent (PK-6101, Vector Laboratories) for 1 h followed by incubation in a diaminobenzidine solution (ImmPACT™ DAB substrate, SK-4105, Vector Laboratories) for 1 min. ..

    Avidin-Biotin Assay:

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: Then the slices were incubated with the second biotinylated antibodies (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) for 30 min. .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Article Title: Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse/human IL-1β, IL-8, TNF-α, CCL2, CCL5, recombinant mouse IL-33 (rmIL-33), recombinant human IL-33 (rhIL-33), monoclonal anti-mouse IL-33-phycoerythrin conjugated Rat IgG2A, monoclonal anti-mouse CD40/TNFRSF5-Allophycocyanin conjugated Rat IgG2A, monoclonal anti-mouse CD40L/TNFSF5 Phycoerythrin conjugated Rat IgG2A antibodies and flow cytometry reagents were obtained from R & D Systems (Minneapolis, MN). .. ImmPRESS reagent anti-mouse Ig peroxidase, ImmPRESS reagent anti-rabbit Ig peroxidase kits, ImmPACT DAB peroxidase and avidin-biotin complex (ABC) kits were obtained from Vector Laboratories (Burlingame, CA). .. Recombinant α-synuclein (human) was from Enzo Life Sciences (Farmingdale, NY) and used with both human and mouse mast cells.

    Staining:

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: Then the slices were incubated with the second biotinylated antibodies (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) for 30 min. .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Modification:

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Article Snippet: .. Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112) .. Incubator Centrifuge Cell culture laminar flow hood, vacuum suction, centrifuge, cell counter 2 L induction chamber Scissors (Roboz Surgical Instrument, catalog number: RS-5914) Fine forceps (Roboz Surgical Instrument, catalog number: RS-5132) Mouse housing and handling facility Isoflurane gas anesthetic system (VetEquip, catalog number: 922100) including a vaporizer, induction chamber, tubing, nose cones, and oxygen tank Glass bead sterilizer (GERMINATOR 500) (Roboz Surgical Instrument, catalog number: DS-401) Rechargeable shaver Space gel heating pads (Braintree Scientific, catalog number: SPGL) 25 μl, model 702 RN syringe (Hamilton, catalog number: 7636-01) Syringe guide 25 μl–500 μl/7000 SYR (Hamilton, catalog number: 14906)

    Recombinant:

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Article Snippet: .. Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112) .. Incubator Centrifuge Cell culture laminar flow hood, vacuum suction, centrifuge, cell counter 2 L induction chamber Scissors (Roboz Surgical Instrument, catalog number: RS-5914) Fine forceps (Roboz Surgical Instrument, catalog number: RS-5132) Mouse housing and handling facility Isoflurane gas anesthetic system (VetEquip, catalog number: 922100) including a vaporizer, induction chamber, tubing, nose cones, and oxygen tank Glass bead sterilizer (GERMINATOR 500) (Roboz Surgical Instrument, catalog number: DS-401) Rechargeable shaver Space gel heating pads (Braintree Scientific, catalog number: SPGL) 25 μl, model 702 RN syringe (Hamilton, catalog number: 7636-01) Syringe guide 25 μl–500 μl/7000 SYR (Hamilton, catalog number: 14906)

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  • 99
    Vector Laboratories immpact dab peroxidase substrate
    Myc-CaP orthotopic allograft and staining by H E and IHC A. Myc-CaP orthotopic allograft (arrow) in the mouse anterior prostate. Seminal vesicle (S.V.) and anterior prostate (A.P.). H E and IHC staining for Myc and androgen receptor (AR) of a Myc-CaP orthotopic allograft tumor; B. H E and IHC staining of a Myc-CaP orthotopic allograft tumor (T) and adjacent seminal vesicle containing secretions (S, bright pink staining); C. H E and IHC staining of a lung metastasis from a Myc-CaP orthotopic allograft. Positive Myc and AR staining, shown in brown, was visualized with an ImmPRESS Polymer Detection Kit and <t>ImmPACT</t> <t>DAB</t> (Vector Laboratories). IHC slides were counterstained with hematoxylin. Images at 10× magnification (20× inset) and black bars are 100 μm.
    Immpact Dab Peroxidase Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immpact dab peroxidase substrate/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immpact dab peroxidase substrate - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    94
    Vector Laboratories diamino benzidine
    Immunoreactive VISTA is detected in human synovium tissue sections and lysates of normal and rheumatoid human synovium. a – c Normal human synovium. d – l RA synovium. a , d , g , j Hematoxylin-and-eosin-stained paraffin tissue sections. b , e , h , k Anti-VISTA peroxidase staining developed with <t>diamino-benzidine.</t> e Arrows denote synovial membrane staining; rectangle encloses area enlarged in ( g – i ). c , f , i , l Nonspecific isotype control antibody staining developed with diamino-benzidine. j – l Neutrophils in vessel in synovium; arrows denote VISTA-stained neutrophils in ( k ). m Western blot of synovial tissue lysates from rheumatoid arthritis patients ( n = 3) and normal synovium ( n = 2) stained with anti-human VISTA antibody or anti-histone H3 as sample loading control. VISTA; V domain-containing Ig suppressor of T-cell activation
    Diamino Benzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diamino benzidine/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diamino benzidine - by Bioz Stars, 2021-05
    94/100 stars
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    Image Search Results


    Myc-CaP orthotopic allograft and staining by H E and IHC A. Myc-CaP orthotopic allograft (arrow) in the mouse anterior prostate. Seminal vesicle (S.V.) and anterior prostate (A.P.). H E and IHC staining for Myc and androgen receptor (AR) of a Myc-CaP orthotopic allograft tumor; B. H E and IHC staining of a Myc-CaP orthotopic allograft tumor (T) and adjacent seminal vesicle containing secretions (S, bright pink staining); C. H E and IHC staining of a lung metastasis from a Myc-CaP orthotopic allograft. Positive Myc and AR staining, shown in brown, was visualized with an ImmPRESS Polymer Detection Kit and ImmPACT DAB (Vector Laboratories). IHC slides were counterstained with hematoxylin. Images at 10× magnification (20× inset) and black bars are 100 μm.

    Journal: Bio-protocol

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis

    doi: 10.21769/BioProtoc.2137

    Figure Lengend Snippet: Myc-CaP orthotopic allograft and staining by H E and IHC A. Myc-CaP orthotopic allograft (arrow) in the mouse anterior prostate. Seminal vesicle (S.V.) and anterior prostate (A.P.). H E and IHC staining for Myc and androgen receptor (AR) of a Myc-CaP orthotopic allograft tumor; B. H E and IHC staining of a Myc-CaP orthotopic allograft tumor (T) and adjacent seminal vesicle containing secretions (S, bright pink staining); C. H E and IHC staining of a lung metastasis from a Myc-CaP orthotopic allograft. Positive Myc and AR staining, shown in brown, was visualized with an ImmPRESS Polymer Detection Kit and ImmPACT DAB (Vector Laboratories). IHC slides were counterstained with hematoxylin. Images at 10× magnification (20× inset) and black bars are 100 μm.

    Article Snippet: Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112)

    Techniques: Staining, Immunohistochemistry, Plasmid Preparation

    Expression of VEGF-A in breast IDC and breast FA. Expression of VEGF-A in IDC samples before (a) and after (b) incubation with polyclonal activator (PA). Expression of VEGF-A in FA samples before (c) and after (d) incubation with PA. Immunohistochemical staining with 3,3′-diaminobenzidine (DAB; brown) as a substrate and additional staining with H E (400× magnification).

    Journal: International Journal of Immunopathology and Pharmacology

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors

    doi: 10.1177/0394632016681306

    Figure Lengend Snippet: Expression of VEGF-A in breast IDC and breast FA. Expression of VEGF-A in IDC samples before (a) and after (b) incubation with polyclonal activator (PA). Expression of VEGF-A in FA samples before (c) and after (d) incubation with PA. Immunohistochemical staining with 3,3′-diaminobenzidine (DAB; brown) as a substrate and additional staining with H E (400× magnification).

    Article Snippet: The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E.

    Techniques: Expressing, Incubation, Immunohistochemistry, Staining

    The expression of phosphorylated CREB (pCREB) in the dorsal horn is attenuated in BLT1KO mice. Representative diaminobenzidine-stained images showing pCREB-positive neurons in sections of the ipsilateral (A) and contralateral (B) dorsal horn of the lumbar spinal cord before or 20 min after a peripheral formalin injection (magnification, 100×). Arrowheads indicate pCREB-positive neurons. The number of pCREB-positive neurons in the ipsilateral (C) and contralateral (D) dorsal horn of the lumbar spinal cord prior to or 20 min after formalin injections. (**** p

    Journal: Molecular Pain

    Article Title: The absence of the leukotriene B4 receptor BLT1 attenuates peripheral inflammation and spinal nociceptive processing following intraplantar formalin injury

    doi: 10.1186/s12990-015-0010-9

    Figure Lengend Snippet: The expression of phosphorylated CREB (pCREB) in the dorsal horn is attenuated in BLT1KO mice. Representative diaminobenzidine-stained images showing pCREB-positive neurons in sections of the ipsilateral (A) and contralateral (B) dorsal horn of the lumbar spinal cord before or 20 min after a peripheral formalin injection (magnification, 100×). Arrowheads indicate pCREB-positive neurons. The number of pCREB-positive neurons in the ipsilateral (C) and contralateral (D) dorsal horn of the lumbar spinal cord prior to or 20 min after formalin injections. (**** p

    Article Snippet: The sections were incubated with biotinylated goat anti-rabbit IgG (1:200, Vector Laboratories, Burlingame, CA, USA) at room temperature for 1 h and then incubated in Vectastain Elite ABC Reagent (PK-6101, Vector Laboratories) for 1 h followed by incubation in a diaminobenzidine solution (ImmPACT™ DAB substrate, SK-4105, Vector Laboratories) for 1 min.

    Techniques: Expressing, Mouse Assay, Staining, Injection

    Immunoreactive VISTA is detected in human synovium tissue sections and lysates of normal and rheumatoid human synovium. a – c Normal human synovium. d – l RA synovium. a , d , g , j Hematoxylin-and-eosin-stained paraffin tissue sections. b , e , h , k Anti-VISTA peroxidase staining developed with diamino-benzidine. e Arrows denote synovial membrane staining; rectangle encloses area enlarged in ( g – i ). c , f , i , l Nonspecific isotype control antibody staining developed with diamino-benzidine. j – l Neutrophils in vessel in synovium; arrows denote VISTA-stained neutrophils in ( k ). m Western blot of synovial tissue lysates from rheumatoid arthritis patients ( n = 3) and normal synovium ( n = 2) stained with anti-human VISTA antibody or anti-histone H3 as sample loading control. VISTA; V domain-containing Ig suppressor of T-cell activation

    Journal: Arthritis Research & Therapy

    Article Title: VISTA deficiency attenuates antibody-induced arthritis and alters macrophage gene expression in response to simulated immune complexes

    doi: 10.1186/s13075-017-1474-y

    Figure Lengend Snippet: Immunoreactive VISTA is detected in human synovium tissue sections and lysates of normal and rheumatoid human synovium. a – c Normal human synovium. d – l RA synovium. a , d , g , j Hematoxylin-and-eosin-stained paraffin tissue sections. b , e , h , k Anti-VISTA peroxidase staining developed with diamino-benzidine. e Arrows denote synovial membrane staining; rectangle encloses area enlarged in ( g – i ). c , f , i , l Nonspecific isotype control antibody staining developed with diamino-benzidine. j – l Neutrophils in vessel in synovium; arrows denote VISTA-stained neutrophils in ( k ). m Western blot of synovial tissue lysates from rheumatoid arthritis patients ( n = 3) and normal synovium ( n = 2) stained with anti-human VISTA antibody or anti-histone H3 as sample loading control. VISTA; V domain-containing Ig suppressor of T-cell activation

    Article Snippet: Signal amplification was performed with Biotin-XX Tyramide SuperBoost (catalog number B40921; Invitrogen) followed by reaction with streptavidin horseradish peroxidase (catalog number S911; Invitrogen) and insoluble substrate development with diamino-benzidine (ImmPact DAB, catalog number SK-4103; Vector Laboratories).

    Techniques: Staining, Western Blot, Activation Assay