immobilon p hydrophobic pvdf plates  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Immobilon P Polyvinylidene difluoride membranes
    Description:

    Catalog Number:
    p2938
    Price:
    None
    Applications:
    Immobilon-P Polyvinylidene difluoride membrane has been used in western blotting for the transfer of proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
    Buy from Supplier


    Structured Review

    Millipore immobilon p hydrophobic pvdf plates

    https://www.bioz.com/result/immobilon p hydrophobic pvdf plates/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immobilon p hydrophobic pvdf plates - by Bioz Stars, 2020-12
    99/100 stars

    Images

    Related Articles

    Electrophoresis:

    Article Title: The ClpXP ATP-Dependent Protease Regulates Flagellum Synthesis in Salmonella enterica Serovar Typhimurium
    Article Snippet: .. For immunoblot analysis, proteins separated on the SDS-12% polyacrylamide gels were transferred after electrophoresis onto Immobilon-P (Millipore) as previously reported ( ). .. Proteins were reacted with either rabbit anti- S. enterica serovar Typhimurium FliC (1:25,000) or FliA (1:12,500) antiserum, followed by treatment with alkaline phosphatase-conjugated anti-rabbit immunoglobulin G. The enzymatic reactions were performed in the presence of 300 μg of nitroblue tetrazolium (Dojindo, Kumamoto, Japan)/ml and 150 μg of bromochloroindolyl phosphate (aMReSCO, Solon, Ohio)/ml.

    Article Title: Catalytic mechanism of the phospholipase D superfamily proceeds via a covalent phosphohistidine intermediate
    Article Snippet: .. The gel was soaked in buffer containing 12.5 mM Tris base, 96 mM glycine, and 20% methanol, and the protein was transferred to an Immobilon P (Millipore) membrane by electrophoresis at 100 V for 1 hr in the same buffer. .. The four lanes containing equal amounts of radioactivity were cut into strips and counted in a Beckman scintillation counter.

    Western Blot:

    Article Title: Characterization of the Golgi Complex Cleared of Proteins in Transit and Examination of Calcium Uptake Activities
    Article Snippet: .. For immunoblots, samples were transferred to Immobilon-P (Millipore, Bedford, MA) and were blocked for 1 h in 5% defatted milk/PBS/0.02% sodium azide. .. When using a mouse primary antibody, the filters were incubated with rabbit anti-mouse IgG for 2 h. Bound antibody was detected using 125 I-labeled protein A (Dupont, New England Nuclear, Boston, MA).

    Transferring:

    Article Title: DECREASED ENZYME ACTIVITIES OF CHAPERONES PDI AND BIP IN AGED MOUSE LIVERS
    Article Snippet: .. Fractions containing PDI and BiP were identified by resolving a small aliquot of these fractions on large format 26 well acrylamide gels (Bio-Rad), transferring the gels to Immobilon-P (Millipore) PVDF membranes and probing membranes with antibodies specific for either PDI or BiP. .. Protein samples containing PDI and BiP were pooled and applied to a 2 ml Bio-Scale ceramic hydroxyapatite (HAP) column (Bio-Rad).

    Activity Assay:

    Article Title: Characterization of the autophosphorylation property of HflX, a ribosome‐binding GTPase from Escherichia coli
    Article Snippet: .. The Ni+2 ‐nitrilotriacetic acid resin (QIAGEN GmbH, Hilden, Germany), dNTP mix (Fermentas Life Sciences, Waltham, MA, USA), Immobilon‐P (PVDF, polyvinylidene fluoride) membrane (Millipore, Billerica, MA, USA) and [γ32 ‐P] GTP (specific activity 4500 Ci·mmol−1 ) (BRIT, Mumbai, India) were purchased from the indicated sources. .. Cellulose thin layer (100 μm) chromatography plates (polyester sigmacell type 100 cellulose) of dimensions 20 cm × 20 cm, were purchased from Sigma.

    SDS Page:

    Article Title: The Long Form of Fas Apoptotic Inhibitory Molecule Is Expressed Specifically in Neurons and Protects Them against Death Receptor-Triggered Apoptosis
    Article Snippet: .. Cell lysates (10–25 μg of protein) were resolved in SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) Immobilon-P membranes (Millipore, Bedford, MA) using a semidry transfer unit (Hoefer Pharmacia Biotech, San Francisco, CA). .. After blocking with Tris-buffered saline with Tween-20 containing 5% nonfat dry milk for 1 h at room temperature, the membranes were probed with the appropriate primary antibodies according to the specific requirements indicated by each provider.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore elispot plates
    Number of brain MNCs and their CNS antigen-specific autoreactive phenotype 14 d after MCAo. A , Number of MNCs infiltrating ipsilateral and contralateral hemisphere of the brain in vehicle and SNS/HPA blockade group (three individual experiments; vehicle, N = 15; SNS/HPA block, N = 20). B–D , <t>Elispot</t> experiments. B , Higher number of total brain MNCs responded to pMOG 35–55 stimulation with IFN-γ production (Th1 cells) per equal number of brain MNCs in SNS/HPA block group compared with the vehicle group. C , A similar effect was observed in ipsilateral hemisphere MNCs when only the SNS axis of SIDS was blocked with propranolol (two experiments; vehicle group, N = 5–8; SNS block, N = 6 or 7). D , Number of splenocytes reacting to pMOG 35–55 stimulation with IFN-γ, IL-4, or IL-17 secretion did not differ between the groups. E , F , ELISA experiments. TGF-β1, IL-10, IL-4, IL-17, and IFN-γ secretion (pg/ml) by pMOG 35–55 -stimulated brain MNCs, ipsilateral hemisphere ( E ) and by pMOG 35–55 -stimulated splenocytes ( F ).
    Elispot Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elispot plates/product/Millipore
    Average 99 stars, based on 468 article reviews
    Price from $9.99 to $1999.99
    elispot plates - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    91
    Millipore 96 well hydrophobic polyvinylidene difluoride backed plates
    Analysis of frequencies of IFN γ -producing T-cells specific to a model antigen or adenovirus vector. A)  ELIsport analyses of IFNγ-producing T-cells frequencies. C57 or C57-CD46 mice were injected intramuscularly with 5×10 9  pfu of Ad5.HBeAg, Ad5/35.HBeAg, or PBS. Twelve days later, spleen cells of naïve syngeneic animals were obtained and pulsed with 5μg/ml of recombinant HBeAg and HBsAg (as a negative control)  (left panel)  or transduced with 25pfu (=500 viral particles) of Ad.5GFP (right panel). On day 14, vaccinated animals were sacrificed, splenocytes were collected and 1×10 6  cells were mixed with 1×10 6 ex vivo  pulsed splenocytes for  in vitro  sensitization. After 24 hours of incubation in 96 well plates, cells were plated in anti-IFNγ-coated wells of an ELIspot plate. Twenty-four hours later, plates were washed and the spots of IFNγ producing T-cells were counted. The number of spots was expressed as the average +/− the standard deviation. N=3 animals per group. B)  Frequency of IFNγ +  CD4 +  cells (left panel) and IFNγ +  CD8 +  cells (right panel) analyzed by intracellular cytokine staining .  C57-CD46 mice were injected intramuscularly with 5×10 9  pfu of Ad5/35.HBeAg, or PBS. Twelve days after immunization, splenocytes were harvested and cultured with recombinant HBeAg and (control) HBsAg for 5 hours in the presence of Brefeldin. Cells were then stained for CD4 and CD8 and intracellular IFNγ. Shown is the percentage of IFNγ + CD4 +  in all CD4 +  cells and IFNγ + CD8 +  in all CD8 +  cells. (N=3)
    96 Well Hydrophobic Polyvinylidene Difluoride Backed Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well hydrophobic polyvinylidene difluoride backed plates/product/Millipore
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    96 well hydrophobic polyvinylidene difluoride backed plates - by Bioz Stars, 2020-12
    91/100 stars
      Buy from Supplier

    85
    Millipore multiscreen ip sterile 96 well filtration plates
    Analysis of frequencies of IFN γ -producing T-cells specific to a model antigen or adenovirus vector. A)  ELIsport analyses of IFNγ-producing T-cells frequencies. C57 or C57-CD46 mice were injected intramuscularly with 5×10 9  pfu of Ad5.HBeAg, Ad5/35.HBeAg, or PBS. Twelve days later, spleen cells of naïve syngeneic animals were obtained and pulsed with 5μg/ml of recombinant HBeAg and HBsAg (as a negative control)  (left panel)  or transduced with 25pfu (=500 viral particles) of Ad.5GFP (right panel). On day 14, vaccinated animals were sacrificed, splenocytes were collected and 1×10 6  cells were mixed with 1×10 6 ex vivo  pulsed splenocytes for  in vitro  sensitization. After 24 hours of incubation in 96 well plates, cells were plated in anti-IFNγ-coated wells of an ELIspot plate. Twenty-four hours later, plates were washed and the spots of IFNγ producing T-cells were counted. The number of spots was expressed as the average +/− the standard deviation. N=3 animals per group. B)  Frequency of IFNγ +  CD4 +  cells (left panel) and IFNγ +  CD8 +  cells (right panel) analyzed by intracellular cytokine staining .  C57-CD46 mice were injected intramuscularly with 5×10 9  pfu of Ad5/35.HBeAg, or PBS. Twelve days after immunization, splenocytes were harvested and cultured with recombinant HBeAg and (control) HBsAg for 5 hours in the presence of Brefeldin. Cells were then stained for CD4 and CD8 and intracellular IFNγ. Shown is the percentage of IFNγ + CD4 +  in all CD4 +  cells and IFNγ + CD8 +  in all CD8 +  cells. (N=3)
    Multiscreen Ip Sterile 96 Well Filtration Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiscreen ip sterile 96 well filtration plates/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multiscreen ip sterile 96 well filtration plates - by Bioz Stars, 2020-12
    85/100 stars
      Buy from Supplier

    Image Search Results


    Number of brain MNCs and their CNS antigen-specific autoreactive phenotype 14 d after MCAo. A , Number of MNCs infiltrating ipsilateral and contralateral hemisphere of the brain in vehicle and SNS/HPA blockade group (three individual experiments; vehicle, N = 15; SNS/HPA block, N = 20). B–D , Elispot experiments. B , Higher number of total brain MNCs responded to pMOG 35–55 stimulation with IFN-γ production (Th1 cells) per equal number of brain MNCs in SNS/HPA block group compared with the vehicle group. C , A similar effect was observed in ipsilateral hemisphere MNCs when only the SNS axis of SIDS was blocked with propranolol (two experiments; vehicle group, N = 5–8; SNS block, N = 6 or 7). D , Number of splenocytes reacting to pMOG 35–55 stimulation with IFN-γ, IL-4, or IL-17 secretion did not differ between the groups. E , F , ELISA experiments. TGF-β1, IL-10, IL-4, IL-17, and IFN-γ secretion (pg/ml) by pMOG 35–55 -stimulated brain MNCs, ipsilateral hemisphere ( E ) and by pMOG 35–55 -stimulated splenocytes ( F ).

    Journal: The Journal of Neuroscience

    Article Title: Blocking Stroke-Induced Immunodeficiency Increases CNS Antigen-Specific Autoreactivity But Does Not Worsen Functional Outcome after Experimental Stroke

    doi: 10.1523/JNEUROSCI.1532-14.2015

    Figure Lengend Snippet: Number of brain MNCs and their CNS antigen-specific autoreactive phenotype 14 d after MCAo. A , Number of MNCs infiltrating ipsilateral and contralateral hemisphere of the brain in vehicle and SNS/HPA blockade group (three individual experiments; vehicle, N = 15; SNS/HPA block, N = 20). B–D , Elispot experiments. B , Higher number of total brain MNCs responded to pMOG 35–55 stimulation with IFN-γ production (Th1 cells) per equal number of brain MNCs in SNS/HPA block group compared with the vehicle group. C , A similar effect was observed in ipsilateral hemisphere MNCs when only the SNS axis of SIDS was blocked with propranolol (two experiments; vehicle group, N = 5–8; SNS block, N = 6 or 7). D , Number of splenocytes reacting to pMOG 35–55 stimulation with IFN-γ, IL-4, or IL-17 secretion did not differ between the groups. E , F , ELISA experiments. TGF-β1, IL-10, IL-4, IL-17, and IFN-γ secretion (pg/ml) by pMOG 35–55 -stimulated brain MNCs, ipsilateral hemisphere ( E ) and by pMOG 35–55 -stimulated splenocytes ( F ).

    Article Snippet: For this, Elispot plates (96-well plates with hydrophobic PVDF membrane, 0.45 μm pore size; EMD Millipore) were coated overnight with 5 μg/ml of the following: (1) IFN-γ (for Th1 cells; eBioscience, catalog #14-7313-85; RRID:AB_468472); (2) IL-4 (for Th2 cells; BD Biosciences, catalog #551878; RRID:AB_2336921); or (3) IL-17 (for Th17 cells; eBioscience, catalog #16-7175-85; RRID:AB_763573) specific capture antibody in sterile PBS.

    Techniques: Blocking Assay, Enzyme-linked Immunospot, Enzyme-linked Immunosorbent Assay

    Analysis of frequencies of IFN γ -producing T-cells specific to a model antigen or adenovirus vector. A)  ELIsport analyses of IFNγ-producing T-cells frequencies. C57 or C57-CD46 mice were injected intramuscularly with 5×10 9  pfu of Ad5.HBeAg, Ad5/35.HBeAg, or PBS. Twelve days later, spleen cells of naïve syngeneic animals were obtained and pulsed with 5μg/ml of recombinant HBeAg and HBsAg (as a negative control)  (left panel)  or transduced with 25pfu (=500 viral particles) of Ad.5GFP (right panel). On day 14, vaccinated animals were sacrificed, splenocytes were collected and 1×10 6  cells were mixed with 1×10 6 ex vivo  pulsed splenocytes for  in vitro  sensitization. After 24 hours of incubation in 96 well plates, cells were plated in anti-IFNγ-coated wells of an ELIspot plate. Twenty-four hours later, plates were washed and the spots of IFNγ producing T-cells were counted. The number of spots was expressed as the average +/− the standard deviation. N=3 animals per group. B)  Frequency of IFNγ +  CD4 +  cells (left panel) and IFNγ +  CD8 +  cells (right panel) analyzed by intracellular cytokine staining .  C57-CD46 mice were injected intramuscularly with 5×10 9  pfu of Ad5/35.HBeAg, or PBS. Twelve days after immunization, splenocytes were harvested and cultured with recombinant HBeAg and (control) HBsAg for 5 hours in the presence of Brefeldin. Cells were then stained for CD4 and CD8 and intracellular IFNγ. Shown is the percentage of IFNγ + CD4 +  in all CD4 +  cells and IFNγ + CD8 +  in all CD8 +  cells. (N=3)

    Journal: Molecular therapy : the journal of the American Society of Gene Therapy

    Article Title: Evaluation of adenovirus vectors containing serotype 35 fibers for vaccination.

    doi: 10.1016/j.ymthe.2005.12.008

    Figure Lengend Snippet: Analysis of frequencies of IFN γ -producing T-cells specific to a model antigen or adenovirus vector. A) ELIsport analyses of IFNγ-producing T-cells frequencies. C57 or C57-CD46 mice were injected intramuscularly with 5×10 9 pfu of Ad5.HBeAg, Ad5/35.HBeAg, or PBS. Twelve days later, spleen cells of naïve syngeneic animals were obtained and pulsed with 5μg/ml of recombinant HBeAg and HBsAg (as a negative control) (left panel) or transduced with 25pfu (=500 viral particles) of Ad.5GFP (right panel). On day 14, vaccinated animals were sacrificed, splenocytes were collected and 1×10 6 cells were mixed with 1×10 6 ex vivo pulsed splenocytes for in vitro sensitization. After 24 hours of incubation in 96 well plates, cells were plated in anti-IFNγ-coated wells of an ELIspot plate. Twenty-four hours later, plates were washed and the spots of IFNγ producing T-cells were counted. The number of spots was expressed as the average +/− the standard deviation. N=3 animals per group. B) Frequency of IFNγ + CD4 + cells (left panel) and IFNγ + CD8 + cells (right panel) analyzed by intracellular cytokine staining . C57-CD46 mice were injected intramuscularly with 5×10 9 pfu of Ad5/35.HBeAg, or PBS. Twelve days after immunization, splenocytes were harvested and cultured with recombinant HBeAg and (control) HBsAg for 5 hours in the presence of Brefeldin. Cells were then stained for CD4 and CD8 and intracellular IFNγ. Shown is the percentage of IFNγ + CD4 + in all CD4 + cells and IFNγ + CD8 + in all CD8 + cells. (N=3)

    Article Snippet: After 24 hours of incubation in 96 well plates, cells were plated at a concentration of 105 cells/100 μl into wells of 96-well hydrophobic polyvinylidene difluoride-backed plates (Millipore, Bedford, Mass.), previously coated with 50μl of 10μg/ml anti-IFNγ monoclonal antibody (1-D1K, mouse immunoglobulin G1; Mabtech, Nacka, Sweden) overnight at 4°C.

    Techniques: Plasmid Preparation, Mouse Assay, Injection, Recombinant, Negative Control, Transduction, Ex Vivo, In Vitro, Incubation, Enzyme-linked Immunospot, Standard Deviation, Staining, Cell Culture