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GE Healthcare imagequant las 4000 imager
Both the Synt-3A11 and Synt-2C6 monoclonal antibodies interacted specifically with a 32 kDa molecular species of Syntenin-1. ( A ) Ten micrograms of total cell protein from whole-cell lysates of non-malignant PNT1a and PNT2, and 22RV1, DU-145 and LNCaP cancer cell lines were analysed by Western blotting using Synt-3A11 or Synt-2C6 (1 μg/mL) antibodies. Full-length blots are shown. ( B ) siRNA (non-specific control, SDCBP or GAPDH ) was transfected into PNT2 cells for 24, 48 or 72 h and Western blotting performed on 10 μg total protein from whole cell lysate. Detection was performed using 1:10,000 anti-mouse HRP, Novex ® ECL chemiluminescent substrate and <t>ImageQuant™</t> <t>LAS</t> 4000 imager. Detection using Synt-3A11, Synt-2C6 were performed using separate SDS-PAGE gels. Uncropped Western blots are contained within Supplementary Materials .
Imagequant Las 4000 Imager, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagequant las 4000 imager/product/GE Healthcare
Average 91 stars, based on 50 article reviews
Price from $9.99 to $1999.99
imagequant las 4000 imager - by Bioz Stars, 2020-07
91/100 stars

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1) Product Images from "A Paradigm in Immunochemistry, Revealed by Monoclonal Antibodies to Spatially Distinct Epitopes on Syntenin-1"

Article Title: A Paradigm in Immunochemistry, Revealed by Monoclonal Antibodies to Spatially Distinct Epitopes on Syntenin-1

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms20236035

Both the Synt-3A11 and Synt-2C6 monoclonal antibodies interacted specifically with a 32 kDa molecular species of Syntenin-1. ( A ) Ten micrograms of total cell protein from whole-cell lysates of non-malignant PNT1a and PNT2, and 22RV1, DU-145 and LNCaP cancer cell lines were analysed by Western blotting using Synt-3A11 or Synt-2C6 (1 μg/mL) antibodies. Full-length blots are shown. ( B ) siRNA (non-specific control, SDCBP or GAPDH ) was transfected into PNT2 cells for 24, 48 or 72 h and Western blotting performed on 10 μg total protein from whole cell lysate. Detection was performed using 1:10,000 anti-mouse HRP, Novex ® ECL chemiluminescent substrate and ImageQuant™ LAS 4000 imager. Detection using Synt-3A11, Synt-2C6 were performed using separate SDS-PAGE gels. Uncropped Western blots are contained within Supplementary Materials .
Figure Legend Snippet: Both the Synt-3A11 and Synt-2C6 monoclonal antibodies interacted specifically with a 32 kDa molecular species of Syntenin-1. ( A ) Ten micrograms of total cell protein from whole-cell lysates of non-malignant PNT1a and PNT2, and 22RV1, DU-145 and LNCaP cancer cell lines were analysed by Western blotting using Synt-3A11 or Synt-2C6 (1 μg/mL) antibodies. Full-length blots are shown. ( B ) siRNA (non-specific control, SDCBP or GAPDH ) was transfected into PNT2 cells for 24, 48 or 72 h and Western blotting performed on 10 μg total protein from whole cell lysate. Detection was performed using 1:10,000 anti-mouse HRP, Novex ® ECL chemiluminescent substrate and ImageQuant™ LAS 4000 imager. Detection using Synt-3A11, Synt-2C6 were performed using separate SDS-PAGE gels. Uncropped Western blots are contained within Supplementary Materials .

Techniques Used: Western Blot, Transfection, SDS Page

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Article Snippet: Blots were imaged with an ImageQuant LAS 4000 imager (GE Healthcare).

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Article Title: A Paradigm in Immunochemistry, Revealed by Monoclonal Antibodies to Spatially Distinct Epitopes on Syntenin-1
Article Snippet: .. Membranes were visualised using Novex® ECL chemiluminescent substrate reagent kit (Life Technologies) and ImageQuant™ LAS 4000 imager, software version 1.2.0.101 (GE Healthcare Pty Ltd., NSW, Australia). .. The intensity of the signal was quantified relative to a reference GAPDH loading control and Amido Black total protein staining (Sigma Aldrich) using AlphaViewSA™ software v3.0 (ProteinSimple Pty Ltd., CA, USA).

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    GE Healthcare imagequant las 4000 imager
    Both the Synt-3A11 and Synt-2C6 monoclonal antibodies interacted specifically with a 32 kDa molecular species of Syntenin-1. ( A ) Ten micrograms of total cell protein from whole-cell lysates of non-malignant PNT1a and PNT2, and 22RV1, DU-145 and LNCaP cancer cell lines were analysed by Western blotting using Synt-3A11 or Synt-2C6 (1 μg/mL) antibodies. Full-length blots are shown. ( B ) siRNA (non-specific control, SDCBP or GAPDH ) was transfected into PNT2 cells for 24, 48 or 72 h and Western blotting performed on 10 μg total protein from whole cell lysate. Detection was performed using 1:10,000 anti-mouse HRP, Novex ® ECL chemiluminescent substrate and <t>ImageQuant™</t> <t>LAS</t> 4000 imager. Detection using Synt-3A11, Synt-2C6 were performed using separate SDS-PAGE gels. Uncropped Western blots are contained within Supplementary Materials .
    Imagequant Las 4000 Imager, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imagequant las 4000 imager/product/GE Healthcare
    Average 91 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    imagequant las 4000 imager - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    89
    GE Healthcare ccd camera based device
    Both the Synt-3A11 and Synt-2C6 monoclonal antibodies interacted specifically with a 32 kDa molecular species of Syntenin-1. ( A ) Ten micrograms of total cell protein from whole-cell lysates of non-malignant PNT1a and PNT2, and 22RV1, DU-145 and LNCaP cancer cell lines were analysed by Western blotting using Synt-3A11 or Synt-2C6 (1 μg/mL) antibodies. Full-length blots are shown. ( B ) siRNA (non-specific control, SDCBP or GAPDH ) was transfected into PNT2 cells for 24, 48 or 72 h and Western blotting performed on 10 μg total protein from whole cell lysate. Detection was performed using 1:10,000 anti-mouse HRP, Novex ® ECL chemiluminescent substrate and <t>ImageQuant™</t> <t>LAS</t> 4000 imager. Detection using Synt-3A11, Synt-2C6 were performed using separate SDS-PAGE gels. Uncropped Western blots are contained within Supplementary Materials .
    Ccd Camera Based Device, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ccd camera based device/product/GE Healthcare
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ccd camera based device - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

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    Both the Synt-3A11 and Synt-2C6 monoclonal antibodies interacted specifically with a 32 kDa molecular species of Syntenin-1. ( A ) Ten micrograms of total cell protein from whole-cell lysates of non-malignant PNT1a and PNT2, and 22RV1, DU-145 and LNCaP cancer cell lines were analysed by Western blotting using Synt-3A11 or Synt-2C6 (1 μg/mL) antibodies. Full-length blots are shown. ( B ) siRNA (non-specific control, SDCBP or GAPDH ) was transfected into PNT2 cells for 24, 48 or 72 h and Western blotting performed on 10 μg total protein from whole cell lysate. Detection was performed using 1:10,000 anti-mouse HRP, Novex ® ECL chemiluminescent substrate and ImageQuant™ LAS 4000 imager. Detection using Synt-3A11, Synt-2C6 were performed using separate SDS-PAGE gels. Uncropped Western blots are contained within Supplementary Materials .

    Journal: International Journal of Molecular Sciences

    Article Title: A Paradigm in Immunochemistry, Revealed by Monoclonal Antibodies to Spatially Distinct Epitopes on Syntenin-1

    doi: 10.3390/ijms20236035

    Figure Lengend Snippet: Both the Synt-3A11 and Synt-2C6 monoclonal antibodies interacted specifically with a 32 kDa molecular species of Syntenin-1. ( A ) Ten micrograms of total cell protein from whole-cell lysates of non-malignant PNT1a and PNT2, and 22RV1, DU-145 and LNCaP cancer cell lines were analysed by Western blotting using Synt-3A11 or Synt-2C6 (1 μg/mL) antibodies. Full-length blots are shown. ( B ) siRNA (non-specific control, SDCBP or GAPDH ) was transfected into PNT2 cells for 24, 48 or 72 h and Western blotting performed on 10 μg total protein from whole cell lysate. Detection was performed using 1:10,000 anti-mouse HRP, Novex ® ECL chemiluminescent substrate and ImageQuant™ LAS 4000 imager. Detection using Synt-3A11, Synt-2C6 were performed using separate SDS-PAGE gels. Uncropped Western blots are contained within Supplementary Materials .

    Article Snippet: Membranes were visualised using Novex® ECL chemiluminescent substrate reagent kit (Life Technologies) and ImageQuant™ LAS 4000 imager, software version 1.2.0.101 (GE Healthcare Pty Ltd., NSW, Australia).

    Techniques: Western Blot, Transfection, SDS Page