Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Cells were treated with increasing concentrations of E7820 (0.06, 0.125, 0.25, 0.5, 1, 2.5, 5, 7.5, 10, 15, 20, and 23 μM), and cell viability was assessed. ( A ) K562S, ( B ) K562R-ima, and ( C ) K562R+ima cells were treated with E7820 and compared to untreated controls. ( n = 4) (* p < 0.05, *** p < 0.001, **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques:

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Protein levels of ITGA2 were analyzed by flow cytometry. Columns indicate mean fluorescence intensity in ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima. ( n = 4) (*** p < 0.001 and **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Flow Cytometry, Fluorescence

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Flow cytometric analysis using Annexin V-PE/7-AAD-stained K562S and K562R cells. Quantification of apoptotic populations (bar graph) in ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima cells; ( D ) Dot plot representation of flow cytometry data. ( n = 4) (** p < 0.01, and **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Staining, Flow Cytometry

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Effects of E7820 (2.5 μM) on caspase3/7 activity in treated K562S and K562R cells compared to their untreated counterparts. ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima cells ( n = 4) (** p < 0.01 and **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Activity Assay

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Gene expression analysis of K562S and K562R cells upon incubation with E7820 for 72 h by real-time PCR assay. Effects of E7820 treatment BAX , BAD , BIM , and BCL-2 gene expressions; ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima cell; ( D ) Bar graph presentation of BAX/BCL-2 mRNA ratio. ( n = 4) (* p < 0.05, *** p < 0.001, **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Gene Expression, Incubation, Real-time Polymerase Chain Reaction

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Intracellular accumulation of Rho-123 was assessed by flow cytometry in K562S, K562R-ima, and K562R+ima cells. ( A ) Flow cytometry results are represented as dot; ( B ) Bar graph representation of flow cytometry data. ( n = 4) (**** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Flow Cytometry