illustra genomiphi v2 dna amplification kit  (GE Healthcare)

 
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    Name:
    illustra GenomiPhi DNA Amplification Kit
    Description:

    Catalog Number:
    25-6600
    Price:
    None
    Score:
    85
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    Structured Review

    GE Healthcare illustra genomiphi v2 dna amplification kit
    Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the <t>Illustra</t> <t>GenomiPhi</t> V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

    https://www.bioz.com/result/illustra genomiphi v2 dna amplification kit/product/GE Healthcare
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 dna amplification kit - by Bioz Stars, 2019-12
    99/100 stars

    Images

    1) Product Images from "Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates"

    Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-15-889

    Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.
    Figure Legend Snippet: Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

    Techniques Used: Multiple Displacement Amplification, Amplification, Whole Genome Amplification, Ligation, Random Primed, Polymerase Chain Reaction

    2) Product Images from "Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing"

    Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

    Journal: GigaScience

    doi: 10.1186/s13742-015-0068-3

    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Figure Legend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Techniques Used: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

    3) Product Images from "Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates"

    Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-15-889

    Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.
    Figure Legend Snippet: Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

    Techniques Used: Multiple Displacement Amplification, Amplification, Whole Genome Amplification, Ligation, Random Primed, Polymerase Chain Reaction

    4) Product Images from "Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing"

    Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

    Journal: GigaScience

    doi: 10.1186/s13742-015-0068-3

    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Figure Legend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Techniques Used: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

    Related Articles

    Diagnostic Assay:

    Article Title: Prenatal diagnostic testing of the Noonan syndrome genes in fetuses with abnormal ultrasound findings
    Article Snippet: In the diagnostic study group, parallel sequencing of the coding regions and splice sites of PTPN11 , KRAS , SOS1 and RAF1 was performed. .. In the anonymized study group, 10 ng of fetal DNA was amplified using the Illustra Genomiphi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), according to the manufacturer's protocol.

    Clone Assay:

    Article Title: Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Article Snippet: Paragraph title: Tracking of individual clones ... DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer.

    Article Title: Diversity and distribution of single-stranded DNA phages in the North Atlantic Ocean
    Article Snippet: Paragraph title: PCR amplification, cloning and sequencing ... DNA from each sample was amplified using isothermal strand displacement with random hexamer primers and Phi29 DNA polymerase (GenomiPhi V2 DNA Amplification Kit; GE Healthcare, Chalfont St Giles, UK) following the manufacturer's protocol.

    Amplification:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: The DNA was further cleaned using the Qiagen gel extraction kit (Qiaex II; catalog no. 20021; Qiagen). .. Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations. .. Library preparation included a 10% phiX V3 spike per the manufacturer's instructions ( ) with the Illumina Nextera XT library prep kit/MiSeq reagent kit V2.

    Article Title: Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Article Snippet: Sequences were mapped to the murine genome using the UCSC Blast-like alignment tool (BLAT) genome browser. .. DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer. .. For subsequent amplification of the unique genomic-proviral junctions, PCR primers were designed.

    Article Title: Sleeping Beauty-Mediated Drug Resistance Gene Transfer in Human Hematopoietic Progenitor Cells
    Article Snippet: Individual progenitor-derived colonies were harvested from methylcellulose cultures and genomic DNA was purified with a Quick-gDNA MicroPrep kit (Zymo Research, Irvine, CA). .. Ten nanograms of genomic DNA (gDNA) was whole-genome amplified with an illustra GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ), and 1 μg of amplified material was evaluated for transposon integrants by linear amplification-mediated PCR (LAM-PCR) as previously described, using primers designed for recovery of SB transposon–chromosome junction sequences. .. The recovered sequences were evaluated by nucleotide–nucleotide Basic Local Alignment Search Tool analysis against the human genome, using the National Center for Biotechnology Information (NCBI, Bethesda, MD) database.

    Article Title: Comprehensive sequence analysis of the NR5A1 gene encoding steroidogenic factor 1 in a large group of infertile males
    Article Snippet: Genomic DNA was extracted from EDTA-preserved blood using the standard techniques. .. All DNA probes were whole-genome amplified by using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences, Chalfont St Giles, UK), according to the manufacturer's description. .. PCR was carried out in a volume of 20 μ l with ∼200 ng DNA and specific primers for all exons of NR5A1 (exons 1–7; ) as described in Supplementary Table 1.

    Article Title: Viruses in a 14th-Century Coprolite
    Article Snippet: A standard 18S ribosomal DNA (rDNA) PCR was performed to verify the absence of human DNA contamination. .. Nucleic acids were amplified in duplicate reactions using the Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Freiburg, Germany). .. A shotgun strategy was chosen for high-throughput pyrosequencing on a 454 Life Sciences Genome FLX sequencer using titanium chemistry (Genome Sequencer RLX; Roche).

    Article Title: Muscle-Bound Primordial Stem Cells Give Rise to Myofiber-Associated Myogenic and Non-Myogenic Progenitors
    Article Snippet: Cells were allowed to proliferate for 14 days, before harvest and DNA isolation. .. WGA was performed using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences, Piscataway, NJ, USA) according to the manufacturer's instructions as described by G. Kumar et al . .. Briefly, cultured clones transferred to PCR tubes (0.2 ml volume) using 3 µl sample buffer from the kit.

    Article Title: Post-Integration Silencing of piggyBac Transposable Elements in Aedes aegypti
    Article Snippet: One thousand cells and 2×104 cells were collected for whole genome amplification and genomic DNA isolation, respectively. .. Whole genome amplifications were performed on cell lysates using the Illustra GenomiPhi V2 DNA Amplification Kit according to the manufacturers recommendations (GE Healthcare). .. Genomic DNA was isolated using a DNeasy kit (Qiagen) after growing the initial 2×104 cell for a week in Eagle’s MEM.

    Article Title: Study of the Viral and Microbial Communities Associated With Crohn's Disease: A Metagenomic Approach
    Article Snippet: Double-stranded cDNA was incubated for 2 min at 95 °C and chilled on ice for 2 min before addition of 5 U of Klenow fragment and incubation at 37 °C for 1 h. Finally, after enzyme inactivation for 10 min at 75 °C, the reactions were purified by ethanol–sodium acetate precipitation. .. To amplify the viral cDNA and genomic DNA for sequencing, a whole genome amplification strategy was carried out using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Amersham, UK), incubating for 2 h at 30 °C followed by phi29 DNA polymerase inactivation for 10 min at 65 °C. .. The resulting DNA amplification was confirmed by fluorometric measurement using Picogreen®, and 1 μg of DNA per sample was taken.

    Article Title: Analysis of archived residual newborn screening blood spots after whole genome amplification
    Article Snippet: DNA was quantified using a Qubit® dsDNA BR Assay Kit (LifeTechnologies). .. 10 ng genomic DNA was then amplified using the GenomiPhi DNA Amplification kit (GE Healthcare) following the manufacturer’s standard protocol. .. Briefly, 1 ul of genomic DNA (diluted to 10 ng/ul) was added to 9 ul sample buffer and was heated to 95 °C for 3 min to denature the template DNA.

    Article Title: Inferring the evolutionary histories of divergences in Hylobates and Nomascus gibbons through multilocus sequence data
    Article Snippet: All DNA samples used derive from the long-term sample collections of the authors and were not acquired specifically for this study. .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. The WGA products were purified by ethanol precipitation following manufacturer’s instructions.

    Article Title: A comparative analysis of Y chromosome and mtDNA phylogenies of the Hylobates gibbons
    Article Snippet: An additional eight DNA samples from females were used to test the male-specificity of the primers as described below. .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. The WGA products were purified by ethanol precipitation following manufacturer’s instructions.

    Article Title: Large duplications at reciprocal translocation breakpoints that might be the counterpart of large deletions and could arise from stalled replication bubbles
    Article Snippet: Briefly, metaphase chromosome suspensions were prepared and separated by standard methods on the basis of size and GC:AT content. .. Typically, 5000 copies of each chromosome were collected, amplified using phi29 DNA polymerase (GenomiPhi kit; GE Healthcare) and the DNA hybridized to genomic DNA arrays essentially as for array CGH. .. The same chromosome fractions were also used to test for the presence of sequences by PCR and for the detection of heterozygous SNPs.

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Simulated 3-D images and sections were generated using the software Volocity and the plan views with side profile slices using IMARIS (Bitplane AG, Zűrich, CH). .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. After amplification, the genomic material was purified away from the agarose matrix using a β-agarase (New England Biolabs) reaction followed by purification using buffer-saturated phenol (Invitrogen) and ethanol precipitation.

    Article Title: Diversity and distribution of single-stranded DNA phages in the North Atlantic Ocean
    Article Snippet: The small-scale filtering protocol was used to extract viral DNA from samples taken in October 2008 at sites BV42-3 (30°40′N, 64°19′W), BV42-5 (28°40′N, 64°37′W) and BV42-9 (24°40′N, 65°13′W). .. DNA from each sample was amplified using isothermal strand displacement with random hexamer primers and Phi29 DNA polymerase (GenomiPhi V2 DNA Amplification Kit; GE Healthcare, Chalfont St Giles, UK) following the manufacturer's protocol. .. It is known that this method preferentially amplifies small, ssDNA circular genomes in mixed communities by two to three orders of magnitude ( ; ); therefore, no attempts were made to quantify the abundance of the ssDNA phage genomes.

    Article Title: WDR11, a WD Protein that Interacts with Transcription Factor EMX1, Is Mutated in Idiopathic Hypogonadotropic Hypogonadism and Kallmann Syndrome
    Article Snippet: Flow-sorting of metaphase chromosomes was performed as described previously. .. Both derivative chromosomes were amplified with the GenomiPhi V2 DNA Amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer's protocol. .. One microgram of each amplification product was labeled with Cy3 or Cy5 via Agilent's Genomic DNA Enzymatic Labeling Kit Plus (Agilent).

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    Article Snippet: Samples not showing a DNA band of at least 20 kb or at 5 to 25 ng DNA not amplifiable by both real-time PCR assays were excluded. .. Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel. .. SIPA1 and RRP1B polymorphisms were characterized using allele-specific PCR.

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: DNA was extracted from the rhizosphere soil along with A. maximus and C. elegans rhizosphere exposed soils and the long term cultures using the FastDNA Spin Kit for Soil (Qbiogene). .. The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. This amplified DNA was used as the template DNA for PCR analysis.

    Article Title: De Novo Loss-of-Function Mutations in SETD5, Encoding a Methyltransferase in a 3p25 Microdeletion Syndrome Critical Region, Cause Intellectual Disability
    Article Snippet: The phenotypes studied were congenital heart disease, ciliopathy, coloboma, ID, neuromuscular disease, severe insulin resistance, and congenital thyroid disease; internal technical control samples were also included for comparison. .. The GenomiPhi V2 DNA Amplification Kit (GE Healthcare) was used for whole-genome amplification of the DNA used for sequence analysis with the use of 1 μl of 10 ng/μl template DNA prior to pull-down. .. A custom-based targeted Agilent SureSelect pull-down array was designed with the SureDesign program (Agilent Technologies).

    Article Title: Metagenomic resolution of microbial functions in deep-sea hydrothermal plumes across the Eastern Lau Spreading Center
    Article Snippet: DNA was extracted from ¼ filters as described previously ( ). .. Multiple displacement amplification of genomic DNA was performed using the illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare, Piscataway, NJ, USA). .. Shotgun sequencing of DNA was performed with Illumina HiSeq2000 PE 100 (Illumina, Inc., San Diego, CA, USA) at the University of Michigan DNA Sequencing Core.

    Whole Genome Amplification:

    Article Title: Muscle-Bound Primordial Stem Cells Give Rise to Myofiber-Associated Myogenic and Non-Myogenic Progenitors
    Article Snippet: Cells were allowed to proliferate for 14 days, before harvest and DNA isolation. .. WGA was performed using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences, Piscataway, NJ, USA) according to the manufacturer's instructions as described by G. Kumar et al . .. Briefly, cultured clones transferred to PCR tubes (0.2 ml volume) using 3 µl sample buffer from the kit.

    Article Title: Post-Integration Silencing of piggyBac Transposable Elements in Aedes aegypti
    Article Snippet: One thousand cells and 2×104 cells were collected for whole genome amplification and genomic DNA isolation, respectively. .. Whole genome amplifications were performed on cell lysates using the Illustra GenomiPhi V2 DNA Amplification Kit according to the manufacturers recommendations (GE Healthcare).

    Article Title: Study of the Viral and Microbial Communities Associated With Crohn's Disease: A Metagenomic Approach
    Article Snippet: Double-stranded cDNA was incubated for 2 min at 95 °C and chilled on ice for 2 min before addition of 5 U of Klenow fragment and incubation at 37 °C for 1 h. Finally, after enzyme inactivation for 10 min at 75 °C, the reactions were purified by ethanol–sodium acetate precipitation. .. To amplify the viral cDNA and genomic DNA for sequencing, a whole genome amplification strategy was carried out using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Amersham, UK), incubating for 2 h at 30 °C followed by phi29 DNA polymerase inactivation for 10 min at 65 °C. .. The resulting DNA amplification was confirmed by fluorometric measurement using Picogreen®, and 1 μg of DNA per sample was taken.

    Article Title: Analysis of archived residual newborn screening blood spots after whole genome amplification
    Article Snippet: 10 ng genomic DNA was then amplified using the GenomiPhi DNA Amplification kit (GE Healthcare) following the manufacturer’s standard protocol. .. The sample was cooled and mixed with 9 ul reaction buffer and 1 ul Phi29 enzyme and incubated at 30 °C for 16–18 h. After amplification, the Phi29 DNA polymerase was heat-inactivated by a 10-min incubation at 65 °C.

    Article Title: Inferring the evolutionary histories of divergences in Hylobates and Nomascus gibbons through multilocus sequence data
    Article Snippet: All DNA samples used derive from the long-term sample collections of the authors and were not acquired specifically for this study. .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. The WGA products were purified by ethanol precipitation following manufacturer’s instructions.

    Article Title: A comparative analysis of Y chromosome and mtDNA phylogenies of the Hylobates gibbons
    Article Snippet: An additional eight DNA samples from females were used to test the male-specificity of the primers as described below. .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. The WGA products were purified by ethanol precipitation following manufacturer’s instructions.

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Paragraph title: Visualization and whole genome amplification ... Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare).

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: Paragraph title: DNA isolation and whole genome amplification ... Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    Article Title: De Novo Loss-of-Function Mutations in SETD5, Encoding a Methyltransferase in a 3p25 Microdeletion Syndrome Critical Region, Cause Intellectual Disability
    Article Snippet: The phenotypes studied were congenital heart disease, ciliopathy, coloboma, ID, neuromuscular disease, severe insulin resistance, and congenital thyroid disease; internal technical control samples were also included for comparison. .. The GenomiPhi V2 DNA Amplification Kit (GE Healthcare) was used for whole-genome amplification of the DNA used for sequence analysis with the use of 1 μl of 10 ng/μl template DNA prior to pull-down. .. A custom-based targeted Agilent SureSelect pull-down array was designed with the SureDesign program (Agilent Technologies).

    Synthesized:

    Article Title: Study of the Viral and Microbial Communities Associated With Crohn's Disease: A Metagenomic Approach
    Article Snippet: The second strand of the cDNA was then synthesized using Klenow fragment polymerase (New England Biolabs, Beverly, MA, USA). .. To amplify the viral cDNA and genomic DNA for sequencing, a whole genome amplification strategy was carried out using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Amersham, UK), incubating for 2 h at 30 °C followed by phi29 DNA polymerase inactivation for 10 min at 65 °C.

    SYBR Green Assay:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: The embedded virion(s) were stained with SYBR Green I and visualized on the slide using Confocal Laser Scanning Microscopy (CLSM) to determine that a single viral particle was present in each agarose droplet. .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare).

    Incubation:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: The virus suspension was treated with proteinase K (Fermentas) at a final concentration of 1 μg/ml at 55°C for 2 h. Seventy microliters of SDS (20%) was added and incubated at 37°C for 1 h. Nucleic acids were purified by performing two rounds of phenol-chloroform-isoamyl alcohol (25:24:1) extraction followed by chloroform-isoamyl alcohol (24:1) phase separation. .. Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Article Title: Post-Integration Silencing of piggyBac Transposable Elements in Aedes aegypti
    Article Snippet: The cells were incubated at 25°C at 5% CO2 for an additional 72 hrs. .. Whole genome amplifications were performed on cell lysates using the Illustra GenomiPhi V2 DNA Amplification Kit according to the manufacturers recommendations (GE Healthcare).

    Article Title: Study of the Viral and Microbial Communities Associated With Crohn's Disease: A Metagenomic Approach
    Article Snippet: Double-stranded cDNA was incubated for 2 min at 95 °C and chilled on ice for 2 min before addition of 5 U of Klenow fragment and incubation at 37 °C for 1 h. Finally, after enzyme inactivation for 10 min at 75 °C, the reactions were purified by ethanol–sodium acetate precipitation. .. To amplify the viral cDNA and genomic DNA for sequencing, a whole genome amplification strategy was carried out using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Amersham, UK), incubating for 2 h at 30 °C followed by phi29 DNA polymerase inactivation for 10 min at 65 °C.

    Article Title: Analysis of archived residual newborn screening blood spots after whole genome amplification
    Article Snippet: After 48 h incubation at −20 °C, samples were centrifuged at 4 °C, 14,000 rpm for 30 min, DNA pellet was washed once in 600 ul 70 % ethanol and resuspended in 50 ul DNA hydration solution (10 mM Tris buffer). .. 10 ng genomic DNA was then amplified using the GenomiPhi DNA Amplification kit (GE Healthcare) following the manufacturer’s standard protocol.

    Gel Extraction:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: The DNA was further cleaned using the Qiagen gel extraction kit (Qiaex II; catalog no. 20021; Qiagen). .. Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Expressing:

    Article Title: Post-Integration Silencing of piggyBac Transposable Elements in Aedes aegypti
    Article Snippet: Cells expressing EGFP and DsRed were sorted using a FACSAria™ cell sorter (BD bioscience). .. Whole genome amplifications were performed on cell lysates using the Illustra GenomiPhi V2 DNA Amplification Kit according to the manufacturers recommendations (GE Healthcare).

    Western Blot:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations. .. Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Flow Cytometry:

    Article Title: WDR11, a WD Protein that Interacts with Transcription Factor EMX1, Is Mutated in Idiopathic Hypogonadotropic Hypogonadism and Kallmann Syndrome
    Article Snippet: Flow-sorting of metaphase chromosomes was performed as described previously. .. Both derivative chromosomes were amplified with the GenomiPhi V2 DNA Amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer's protocol.

    Gas Chromatography:

    Article Title: Large duplications at reciprocal translocation breakpoints that might be the counterpart of large deletions and could arise from stalled replication bubbles
    Article Snippet: Briefly, metaphase chromosome suspensions were prepared and separated by standard methods on the basis of size and GC:AT content. .. Typically, 5000 copies of each chromosome were collected, amplified using phi29 DNA polymerase (GenomiPhi kit; GE Healthcare) and the DNA hybridized to genomic DNA arrays essentially as for array CGH.

    Serial Dilution:

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: The quantity and quality of the isolated DNA was established by ultraviolet spectroscopy, by examination of the product size after agarose gel electrophoresis, and by the ability of the sample to be linearly amplified by real-time PCR in a serial dilution with a set of primers located in an intron of the hydroxymethylbilane synthase on chromosome 11 and thymidine kinase on chromosome 17. .. Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    In Vitro:

    Article Title: Post-Integration Silencing of piggyBac Transposable Elements in Aedes aegypti
    Article Snippet: Paragraph title: piggyBac Transposition Assay in vitro ... Whole genome amplifications were performed on cell lysates using the Illustra GenomiPhi V2 DNA Amplification Kit according to the manufacturers recommendations (GE Healthcare).

    Cell Culture:

    Article Title: Prenatal diagnostic testing of the Noonan syndrome genes in fetuses with abnormal ultrasound findings
    Article Snippet: The number of genes tested depended on the amount of DNA available for testing (chorionic villi or amniotic fluid, cultured or uncultured cells) and the GA of the pregnancy. .. In the anonymized study group, 10 ng of fetal DNA was amplified using the Illustra Genomiphi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), according to the manufacturer's protocol.

    Mutagenesis:

    Article Title: Comprehensive sequence analysis of the NR5A1 gene encoding steroidogenic factor 1 in a large group of infertile males
    Article Snippet: Paragraph title: Mutation analysis ... All DNA probes were whole-genome amplified by using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences, Chalfont St Giles, UK), according to the manufacturer's description.

    Article Title: Prenatal diagnostic testing of the Noonan syndrome genes in fetuses with abnormal ultrasound findings
    Article Snippet: The order of genes tested in case not enough DNA was available ( PTPN11 , KRAS , SOS1 , RAF1 ) is based on the reported mutation frequency in postnatally diagnosed NS cases. .. In the anonymized study group, 10 ng of fetal DNA was amplified using the Illustra Genomiphi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), according to the manufacturer's protocol.

    Generated:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Simulated 3-D images and sections were generated using the software Volocity and the plan views with side profile slices using IMARIS (Bitplane AG, Zűrich, CH). .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare).

    Confocal Laser Scanning Microscopy:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: CLSM was performed with a Leica TCSP5 (Leica Microsystems) with 488 nm laser excitation. .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare).

    DNA Sequencing:

    Article Title: Metagenomic resolution of microbial functions in deep-sea hydrothermal plumes across the Eastern Lau Spreading Center
    Article Snippet: Paragraph title: Extraction of nucleic acids and DNA sequencing ... Multiple displacement amplification of genomic DNA was performed using the illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare, Piscataway, NJ, USA).

    Polymerase Chain Reaction:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations. .. Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Article Title: Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Article Snippet: DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer. .. For subsequent amplification of the unique genomic-proviral junctions, PCR primers were designed.

    Article Title: Sleeping Beauty-Mediated Drug Resistance Gene Transfer in Human Hematopoietic Progenitor Cells
    Article Snippet: Individual progenitor-derived colonies were harvested from methylcellulose cultures and genomic DNA was purified with a Quick-gDNA MicroPrep kit (Zymo Research, Irvine, CA). .. Ten nanograms of genomic DNA (gDNA) was whole-genome amplified with an illustra GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ), and 1 μg of amplified material was evaluated for transposon integrants by linear amplification-mediated PCR (LAM-PCR) as previously described, using primers designed for recovery of SB transposon–chromosome junction sequences. .. The recovered sequences were evaluated by nucleotide–nucleotide Basic Local Alignment Search Tool analysis against the human genome, using the National Center for Biotechnology Information (NCBI, Bethesda, MD) database.

    Article Title: Comprehensive sequence analysis of the NR5A1 gene encoding steroidogenic factor 1 in a large group of infertile males
    Article Snippet: All DNA probes were whole-genome amplified by using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences, Chalfont St Giles, UK), according to the manufacturer's description. .. PCR was carried out in a volume of 20 μ l with ∼200 ng DNA and specific primers for all exons of NR5A1 (exons 1–7; ) as described in Supplementary Table 1.

    Article Title: Prenatal diagnostic testing of the Noonan syndrome genes in fetuses with abnormal ultrasound findings
    Article Snippet: In the anonymized study group, 10 ng of fetal DNA was amplified using the Illustra Genomiphi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), according to the manufacturer's protocol. .. In the anonymized study group, 10 ng of fetal DNA was amplified using the Illustra Genomiphi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), according to the manufacturer's protocol.

    Article Title: Inferring the evolutionary histories of divergences in Hylobates and Nomascus gibbons through multilocus sequence data
    Article Snippet: Paragraph title: Gibbon DNA samples and PCR amplification of 14 autosomal loci ... We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare).

    Article Title: A comparative analysis of Y chromosome and mtDNA phylogenies of the Hylobates gibbons
    Article Snippet: Paragraph title: Gibbon DNA samples and PCR amplification of Y chromosome loci ... We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare).

    Article Title: Large duplications at reciprocal translocation breakpoints that might be the counterpart of large deletions and could arise from stalled replication bubbles
    Article Snippet: Paragraph title: Array painting and PCR and SNP detection on sorted chromosomes ... Typically, 5000 copies of each chromosome were collected, amplified using phi29 DNA polymerase (GenomiPhi kit; GE Healthcare) and the DNA hybridized to genomic DNA arrays essentially as for array CGH.

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. Unincorporated dNTPs and random hexamer primers were removed through column purification according to manufacturer specifications, (PureLink Genomic DNA Purification, Invitrogen) as they would be a source of contamination on downstream reactions, such as sequencing.

    Article Title: Diversity and distribution of single-stranded DNA phages in the North Atlantic Ocean
    Article Snippet: Paragraph title: PCR amplification, cloning and sequencing ... DNA from each sample was amplified using isothermal strand displacement with random hexamer primers and Phi29 DNA polymerase (GenomiPhi V2 DNA Amplification Kit; GE Healthcare, Chalfont St Giles, UK) following the manufacturer's protocol.

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: Paragraph title: DNA extraction and PCR analysis ... The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences).

    DNA Extraction:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: Paragraph title: Sample processing, DNA extraction, and sequencing. ... Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Article Title: Post-Integration Silencing of piggyBac Transposable Elements in Aedes aegypti
    Article Snippet: One thousand cells and 2×104 cells were collected for whole genome amplification and genomic DNA isolation, respectively. .. Whole genome amplifications were performed on cell lysates using the Illustra GenomiPhi V2 DNA Amplification Kit according to the manufacturers recommendations (GE Healthcare).

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: Paragraph title: DNA isolation and whole genome amplification ... Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: Paragraph title: DNA extraction and PCR analysis ... The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences).

    BAC Assay:

    Article Title: Large duplications at reciprocal translocation breakpoints that might be the counterpart of large deletions and could arise from stalled replication bubbles
    Article Snippet: Array painting to custom oligonucleotide arrays from NimbleGen Systems and chromosome 6 tiling path BAC arrays was as described ( ). .. Typically, 5000 copies of each chromosome were collected, amplified using phi29 DNA polymerase (GenomiPhi kit; GE Healthcare) and the DNA hybridized to genomic DNA arrays essentially as for array CGH.

    Multiple Displacement Amplification:

    Article Title: Inferring the evolutionary histories of divergences in Hylobates and Nomascus gibbons through multilocus sequence data
    Article Snippet: All DNA samples used derive from the long-term sample collections of the authors and were not acquired specifically for this study. .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. The WGA products were purified by ethanol precipitation following manufacturer’s instructions.

    Article Title: A comparative analysis of Y chromosome and mtDNA phylogenies of the Hylobates gibbons
    Article Snippet: An additional eight DNA samples from females were used to test the male-specificity of the primers as described below. .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. The WGA products were purified by ethanol precipitation following manufacturer’s instructions.

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Simulated 3-D images and sections were generated using the software Volocity and the plan views with side profile slices using IMARIS (Bitplane AG, Zűrich, CH). .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. After amplification, the genomic material was purified away from the agarose matrix using a β-agarase (New England Biolabs) reaction followed by purification using buffer-saturated phenol (Invitrogen) and ethanol precipitation.

    Article Title: Metagenomic resolution of microbial functions in deep-sea hydrothermal plumes across the Eastern Lau Spreading Center
    Article Snippet: DNA was extracted from ¼ filters as described previously ( ). .. Multiple displacement amplification of genomic DNA was performed using the illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare, Piscataway, NJ, USA). .. Shotgun sequencing of DNA was performed with Illumina HiSeq2000 PE 100 (Illumina, Inc., San Diego, CA, USA) at the University of Michigan DNA Sequencing Core.

    Isolation:

    Article Title: Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Article Snippet: Sequences were mapped to the murine genome using the UCSC Blast-like alignment tool (BLAT) genome browser. .. DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer. .. For subsequent amplification of the unique genomic-proviral junctions, PCR primers were designed.

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: The quantity and quality of the isolated DNA was established by ultraviolet spectroscopy, by examination of the product size after agarose gel electrophoresis, and by the ability of the sample to be linearly amplified by real-time PCR in a serial dilution with a set of primers located in an intron of the hydroxymethylbilane synthase on chromosome 11 and thymidine kinase on chromosome 17. .. Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    Transfection:

    Article Title: Post-Integration Silencing of piggyBac Transposable Elements in Aedes aegypti
    Article Snippet: Transfection efficiency was approximately 10%. .. Whole genome amplifications were performed on cell lysates using the Illustra GenomiPhi V2 DNA Amplification Kit according to the manufacturers recommendations (GE Healthcare).

    Labeling:

    Article Title: WDR11, a WD Protein that Interacts with Transcription Factor EMX1, Is Mutated in Idiopathic Hypogonadotropic Hypogonadism and Kallmann Syndrome
    Article Snippet: Both derivative chromosomes were amplified with the GenomiPhi V2 DNA Amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer's protocol. .. One microgram of each amplification product was labeled with Cy3 or Cy5 via Agilent's Genomic DNA Enzymatic Labeling Kit Plus (Agilent).

    Size-exclusion Chromatography:

    Article Title: Large duplications at reciprocal translocation breakpoints that might be the counterpart of large deletions and could arise from stalled replication bubbles
    Article Snippet: Typically, 5000 copies of each chromosome were collected, amplified using phi29 DNA polymerase (GenomiPhi kit; GE Healthcare) and the DNA hybridized to genomic DNA arrays essentially as for array CGH. .. PCR reactions were carried out in a 25-μL reaction volume containing 15 ng of GenomiPhi-amplified DNA, 0.4 μM each primer, 400 μM each dNTP, HotMaster Taq buffer (containing 2.5 mM MgCl2 ) and 1.25 units of HotMaster Taq DNA polymerase (5 PRIME).

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. Near full length 16S rRNA gene fragments were amplified using the 27F and 1492R universal primers .

    Sequencing:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: Paragraph title: Sample processing, DNA extraction, and sequencing. ... Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Article Title: Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Article Snippet: DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer. .. Tracking PCR was performed on 1 to 50 ng preamplified DNA using the unique genomic primers in combination with a vector-specific primer.

    Article Title: Comprehensive sequence analysis of the NR5A1 gene encoding steroidogenic factor 1 in a large group of infertile males
    Article Snippet: All DNA probes were whole-genome amplified by using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences, Chalfont St Giles, UK), according to the manufacturer's description. .. Exon 4 was divided into two overlapping PCR formats.

    Article Title: Viruses in a 14th-Century Coprolite
    Article Snippet: Paragraph title: Viral metagenomic library preparation and sequencing. ... Nucleic acids were amplified in duplicate reactions using the Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Freiburg, Germany).

    Article Title: Prenatal diagnostic testing of the Noonan syndrome genes in fetuses with abnormal ultrasound findings
    Article Snippet: Paragraph title: Sequence analysis ... In the anonymized study group, 10 ng of fetal DNA was amplified using the Illustra Genomiphi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), according to the manufacturer's protocol.

    Article Title: Study of the Viral and Microbial Communities Associated With Crohn's Disease: A Metagenomic Approach
    Article Snippet: Double-stranded cDNA was incubated for 2 min at 95 °C and chilled on ice for 2 min before addition of 5 U of Klenow fragment and incubation at 37 °C for 1 h. Finally, after enzyme inactivation for 10 min at 75 °C, the reactions were purified by ethanol–sodium acetate precipitation. .. To amplify the viral cDNA and genomic DNA for sequencing, a whole genome amplification strategy was carried out using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Amersham, UK), incubating for 2 h at 30 °C followed by phi29 DNA polymerase inactivation for 10 min at 65 °C. .. The resulting DNA amplification was confirmed by fluorometric measurement using Picogreen®, and 1 μg of DNA per sample was taken.

    Article Title: Large duplications at reciprocal translocation breakpoints that might be the counterpart of large deletions and could arise from stalled replication bubbles
    Article Snippet: Typically, 5000 copies of each chromosome were collected, amplified using phi29 DNA polymerase (GenomiPhi kit; GE Healthcare) and the DNA hybridized to genomic DNA arrays essentially as for array CGH. .. Amplification conditions were: 95°C for 30 sec, 30 cycles of 95°C for 30 sec, 58°C for 30 sec, 72°C for 1 min, followed by a final extension at 72°C for 10 min. PCR primers are given in Supplemental Table 2.

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. After amplification, the genomic material was purified away from the agarose matrix using a β-agarase (New England Biolabs) reaction followed by purification using buffer-saturated phenol (Invitrogen) and ethanol precipitation.

    Article Title: Diversity and distribution of single-stranded DNA phages in the North Atlantic Ocean
    Article Snippet: Paragraph title: PCR amplification, cloning and sequencing ... DNA from each sample was amplified using isothermal strand displacement with random hexamer primers and Phi29 DNA polymerase (GenomiPhi V2 DNA Amplification Kit; GE Healthcare, Chalfont St Giles, UK) following the manufacturer's protocol.

    Article Title: De Novo Loss-of-Function Mutations in SETD5, Encoding a Methyltransferase in a 3p25 Microdeletion Syndrome Critical Region, Cause Intellectual Disability
    Article Snippet: The phenotypes studied were congenital heart disease, ciliopathy, coloboma, ID, neuromuscular disease, severe insulin resistance, and congenital thyroid disease; internal technical control samples were also included for comparison. .. The GenomiPhi V2 DNA Amplification Kit (GE Healthcare) was used for whole-genome amplification of the DNA used for sequence analysis with the use of 1 μl of 10 ng/μl template DNA prior to pull-down. .. A custom-based targeted Agilent SureSelect pull-down array was designed with the SureDesign program (Agilent Technologies).

    Spectroscopy:

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: The quantity and quality of the isolated DNA was established by ultraviolet spectroscopy, by examination of the product size after agarose gel electrophoresis, and by the ability of the sample to be linearly amplified by real-time PCR in a serial dilution with a set of primers located in an intron of the hydroxymethylbilane synthase on chromosome 11 and thymidine kinase on chromosome 17. .. Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    Lysis:

    Article Title: Muscle-Bound Primordial Stem Cells Give Rise to Myofiber-Associated Myogenic and Non-Myogenic Progenitors
    Article Snippet: WGA was performed using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences, Piscataway, NJ, USA) according to the manufacturer's instructions as described by G. Kumar et al . .. WGA was performed using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences, Piscataway, NJ, USA) according to the manufacturer's instructions as described by G. Kumar et al .

    Article Title: Analysis of archived residual newborn screening blood spots after whole genome amplification
    Article Snippet: Briefly, 15 ~ 20 punches of 3 mm disks were digested overnight at 55 °C by 3 ul proteinase K solution (25 mg/ml) in a 1.5 ml Eppendorf tube which containing 600 ul cell lysis solution. .. 10 ng genomic DNA was then amplified using the GenomiPhi DNA Amplification kit (GE Healthcare) following the manufacturer’s standard protocol.

    Purification:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: The virus suspension was treated with proteinase K (Fermentas) at a final concentration of 1 μg/ml at 55°C for 2 h. Seventy microliters of SDS (20%) was added and incubated at 37°C for 1 h. Nucleic acids were purified by performing two rounds of phenol-chloroform-isoamyl alcohol (25:24:1) extraction followed by chloroform-isoamyl alcohol (24:1) phase separation. .. Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Article Title: Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Article Snippet: DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer. .. DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer.

    Article Title: Sleeping Beauty-Mediated Drug Resistance Gene Transfer in Human Hematopoietic Progenitor Cells
    Article Snippet: Individual progenitor-derived colonies were harvested from methylcellulose cultures and genomic DNA was purified with a Quick-gDNA MicroPrep kit (Zymo Research, Irvine, CA). .. Ten nanograms of genomic DNA (gDNA) was whole-genome amplified with an illustra GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ), and 1 μg of amplified material was evaluated for transposon integrants by linear amplification-mediated PCR (LAM-PCR) as previously described, using primers designed for recovery of SB transposon–chromosome junction sequences.

    Article Title: Study of the Viral and Microbial Communities Associated With Crohn's Disease: A Metagenomic Approach
    Article Snippet: Double-stranded cDNA was incubated for 2 min at 95 °C and chilled on ice for 2 min before addition of 5 U of Klenow fragment and incubation at 37 °C for 1 h. Finally, after enzyme inactivation for 10 min at 75 °C, the reactions were purified by ethanol–sodium acetate precipitation. .. To amplify the viral cDNA and genomic DNA for sequencing, a whole genome amplification strategy was carried out using the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Amersham, UK), incubating for 2 h at 30 °C followed by phi29 DNA polymerase inactivation for 10 min at 65 °C.

    Article Title: Inferring the evolutionary histories of divergences in Hylobates and Nomascus gibbons through multilocus sequence data
    Article Snippet: We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare).

    Article Title: A comparative analysis of Y chromosome and mtDNA phylogenies of the Hylobates gibbons
    Article Snippet: We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare).

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. After amplification, the genomic material was purified away from the agarose matrix using a β-agarase (New England Biolabs) reaction followed by purification using buffer-saturated phenol (Invitrogen) and ethanol precipitation.

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. The PCR reaction conditions were as follows; 1× concentration of GoTaq Green PCR Master Mix (Promega), 0.5 µm of both 27F and 1492R primers and at least 20 ng of template DNA in a 50 µl reaction, using the following method: 95°C for 5 min then 30 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 1 min, followed by a final extension step for 10 min at 72°C.

    Plasmid Preparation:

    Article Title: Stable differentiation and clonality of murine long-term hematopoiesis after extended reduced-intensity selection for MGMT P140K transgene expression
    Article Snippet: DNA (0.5-2 ng) isolated from peripheral blood samples was preamplified using the Illustra GenomiPhi DNA Amplification Kit (GE Healthcare, Buckinghamshire, United Kingdom) according to instructions of the manufacturer. .. For subsequent amplification of the unique genomic-proviral junctions, PCR primers were designed.

    Software:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Simulated 3-D images and sections were generated using the software Volocity and the plan views with side profile slices using IMARIS (Bitplane AG, Zűrich, CH). .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare).

    Real-time Polymerase Chain Reaction:

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: Samples not showing a DNA band of at least 20 kb or at 5 to 25 ng DNA not amplifiable by both real-time PCR assays were excluded. .. Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    Agarose Gel Electrophoresis:

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: The quantity and quality of the isolated DNA was established by ultraviolet spectroscopy, by examination of the product size after agarose gel electrophoresis, and by the ability of the sample to be linearly amplified by real-time PCR in a serial dilution with a set of primers located in an intron of the hydroxymethylbilane synthase on chromosome 11 and thymidine kinase on chromosome 17. .. Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. The PCR reaction conditions were as follows; 1× concentration of GoTaq Green PCR Master Mix (Promega), 0.5 µm of both 27F and 1492R primers and at least 20 ng of template DNA in a 50 µl reaction, using the following method: 95°C for 5 min then 30 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 1 min, followed by a final extension step for 10 min at 72°C.

    In Situ:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Simulated 3-D images and sections were generated using the software Volocity and the plan views with side profile slices using IMARIS (Bitplane AG, Zűrich, CH). .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. After amplification, the genomic material was purified away from the agarose matrix using a β-agarase (New England Biolabs) reaction followed by purification using buffer-saturated phenol (Invitrogen) and ethanol precipitation.

    Electrophoresis:

    Article Title: Distinct inherited metastasis susceptibility exists for different breast cancer subtypes: a prognosis study
    Article Snippet: The quantity and quality of the isolated DNA was established by ultraviolet spectroscopy, by examination of the product size after agarose gel electrophoresis, and by the ability of the sample to be linearly amplified by real-time PCR in a serial dilution with a set of primers located in an intron of the hydroxymethylbilane synthase on chromosome 11 and thymidine kinase on chromosome 17. .. Prior to SNP genotyping, 10 ng aliquots of genomic DNA were amplified with the GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA) according to the protocol provided by the manufacturer, typically yielding 4 μg amplifiable genomic DNA with the 20 kb band still visible on gel.

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. The PCR reaction conditions were as follows; 1× concentration of GoTaq Green PCR Master Mix (Promega), 0.5 µm of both 27F and 1492R primers and at least 20 ng of template DNA in a 50 µl reaction, using the following method: 95°C for 5 min then 30 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 1 min, followed by a final extension step for 10 min at 72°C.

    Next-Generation Sequencing:

    Article Title: De Novo Loss-of-Function Mutations in SETD5, Encoding a Methyltransferase in a 3p25 Microdeletion Syndrome Critical Region, Cause Intellectual Disability
    Article Snippet: We performed DNA sequence analysis by using next-generation sequencing methods to investigate the coding sequence of 565 genes ( , available online) from 996 individuals with moderate to severe ID (all samples met DNA quality metrics). .. The GenomiPhi V2 DNA Amplification Kit (GE Healthcare) was used for whole-genome amplification of the DNA used for sequence analysis with the use of 1 μl of 10 ng/μl template DNA prior to pull-down.

    Laser Capture Microdissection:

    Article Title: Sleeping Beauty-Mediated Drug Resistance Gene Transfer in Human Hematopoietic Progenitor Cells
    Article Snippet: Individual progenitor-derived colonies were harvested from methylcellulose cultures and genomic DNA was purified with a Quick-gDNA MicroPrep kit (Zymo Research, Irvine, CA). .. Ten nanograms of genomic DNA (gDNA) was whole-genome amplified with an illustra GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ), and 1 μg of amplified material was evaluated for transposon integrants by linear amplification-mediated PCR (LAM-PCR) as previously described, using primers designed for recovery of SB transposon–chromosome junction sequences. .. The recovered sequences were evaluated by nucleotide–nucleotide Basic Local Alignment Search Tool analysis against the human genome, using the National Center for Biotechnology Information (NCBI, Bethesda, MD) database.

    Random Hexamer Labeling:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. After amplification, the genomic material was purified away from the agarose matrix using a β-agarase (New England Biolabs) reaction followed by purification using buffer-saturated phenol (Invitrogen) and ethanol precipitation.

    Article Title: Diversity and distribution of single-stranded DNA phages in the North Atlantic Ocean
    Article Snippet: The small-scale filtering protocol was used to extract viral DNA from samples taken in October 2008 at sites BV42-3 (30°40′N, 64°19′W), BV42-5 (28°40′N, 64°37′W) and BV42-9 (24°40′N, 65°13′W). .. DNA from each sample was amplified using isothermal strand displacement with random hexamer primers and Phi29 DNA polymerase (GenomiPhi V2 DNA Amplification Kit; GE Healthcare, Chalfont St Giles, UK) following the manufacturer's protocol. .. It is known that this method preferentially amplifies small, ssDNA circular genomes in mixed communities by two to three orders of magnitude ( ; ); therefore, no attempts were made to quantify the abundance of the ssDNA phage genomes.

    Spectrophotometry:

    Article Title: Inferring the evolutionary histories of divergences in Hylobates and Nomascus gibbons through multilocus sequence data
    Article Snippet: We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare).

    Article Title: A comparative analysis of Y chromosome and mtDNA phylogenies of the Hylobates gibbons
    Article Snippet: We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare). .. We performed whole genome amplification (WGA) on all genomic DNA samples using the multiple displacement amplification procedure implemented in the GenomiPhi HY DNA Amplification Kit (GE Healthcare).

    Concentration Assay:

    Article Title: High-Level Diversity of Tailed Phages, Eukaryote-Associated Viruses, and Virophage-Like Elements in the Metaviromes of Antarctic Soils
    Article Snippet: The virus suspension was treated with proteinase K (Fermentas) at a final concentration of 1 μg/ml at 55°C for 2 h. Seventy microliters of SDS (20%) was added and incubated at 37°C for 1 h. Nucleic acids were purified by performing two rounds of phenol-chloroform-isoamyl alcohol (25:24:1) extraction followed by chloroform-isoamyl alcohol (24:1) phase separation. .. Ten nanograms of each sample was then used to perform Phi29 amplification (GenomiPhi HY DNA amplification kit; catalog no. 25-6600-20; GE Healthcare) using the manufacturer's recommendations.

    Article Title: Analysis of archived residual newborn screening blood spots after whole genome amplification
    Article Snippet: 10 ng genomic DNA was then amplified using the GenomiPhi DNA Amplification kit (GE Healthcare) following the manufacturer’s standard protocol. .. The sample was cooled and mixed with 9 ul reaction buffer and 1 ul Phi29 enzyme and incubated at 30 °C for 16–18 h. After amplification, the Phi29 DNA polymerase was heat-inactivated by a 10-min incubation at 65 °C.

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. Near full length 16S rRNA gene fragments were amplified using the 27F and 1492R universal primers .

    DNA Purification:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare). .. After amplification, the genomic material was purified away from the agarose matrix using a β-agarase (New England Biolabs) reaction followed by purification using buffer-saturated phenol (Invitrogen) and ethanol precipitation.

    Staining:

    Article Title: Single Virus Genomics: A New Tool for Virus Discovery
    Article Snippet: The embedded virion(s) were stained with SYBR Green I and visualized on the slide using Confocal Laser Scanning Microscopy (CLSM) to determine that a single viral particle was present in each agarose droplet. .. Once a well was identified as positive, the single viral particle was lysed using heat (94°C) for 3 minutes and its genomic material amplified in situ using the phi29 DNA polymerase and multiple displacement amplification (MDA) reaction, as per manufacturers recommendations (GenomiPhi kit, GE Healthcare).

    Article Title: Culture-Independent Investigation of the Microbiome Associated with the Nematode Acrobeloides maximus
    Article Snippet: The resultant DNA from all samples was then individually amplified using the GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. The PCR reaction conditions were as follows; 1× concentration of GoTaq Green PCR Master Mix (Promega), 0.5 µm of both 27F and 1492R primers and at least 20 ng of template DNA in a 50 µl reaction, using the following method: 95°C for 5 min then 30 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 1 min, followed by a final extension step for 10 min at 72°C.

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    GE Healthcare illustra genomiphi v2 dna amplication kit
    Illustra Genomiphi V2 Dna Amplication Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 dna amplication kit/product/GE Healthcare
    Average 99 stars, based on 3 article reviews
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    illustra genomiphi v2 dna amplication kit - by Bioz Stars, 2019-12
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