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GE Healthcare illustra genomiphi v2 dna amplification kit
A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
Illustra Genomiphi V2 Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illustra genomiphi v2 dna amplification kit/product/GE Healthcare
Average 93 stars, based on 182 article reviews
Price from $9.99 to $1999.99
illustra genomiphi v2 dna amplification kit - by Bioz Stars, 2020-07
93/100 stars

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1) Product Images from "Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing"

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

Journal: GigaScience

doi: 10.1186/s13742-015-0068-3

A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
Figure Legend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

Techniques Used: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

2) Product Images from "Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates"

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates

Journal: BMC Genomics

doi: 10.1186/1471-2164-15-889

Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.
Figure Legend Snippet: Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

Techniques Used: Multiple Displacement Amplification, Amplification, Whole Genome Amplification, Ligation, Random Primed, Polymerase Chain Reaction

3) Product Images from "Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing"

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

Journal: GigaScience

doi: 10.1186/s13742-015-0068-3

A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
Figure Legend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

Techniques Used: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

4) Product Images from "Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates"

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates

Journal: BMC Genomics

doi: 10.1186/1471-2164-15-889

Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.
Figure Legend Snippet: Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

Techniques Used: Multiple Displacement Amplification, Amplification, Whole Genome Amplification, Ligation, Random Primed, Polymerase Chain Reaction

5) Product Images from "Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing"

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

Journal: GigaScience

doi: 10.1186/s13742-015-0068-3

A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
Figure Legend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

Techniques Used: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

Related Articles

Amplification:

Article Title: Involvement of condensin-directed gene associations in the organization and regulation of chromosome territories during the cell cycle
Article Snippet: .. The respective chromosomes were purified using Qiaquick gel extraction Kit (Qiagen) and amplified using Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare). .. The chromosome-specific FISH probes were also generated using polymerase chain reaction (PCR)-amplified DNA fragments.

Article Title: Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
Article Snippet: .. For the F1 family V . rupestris B38 x ‘Chardonnay’, whole genome amplification was performed using 10 ng of DNA and the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare) to obtain 1.0 μg of dried DNA per sample. .. Then, 48-plex GBS library preparation and sequencing was completed, as described previously [ ].

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates
Article Snippet: .. Two widely used MDA-based WGA systems were tested, namely the Illustra GenomiPhi V2 DNA amplification kit (#25-6600-30, GE Healthcare Bio-Sciences Corp., QC, Canada) and the REPLI-g Mini Kit (#150023, Qiagen, Mississauga, ON, Canada). .. The QPLS technology is the principle of the Single Cell WGA Kit (#E2620, New England Biolab, Pickering, ON, Canada).

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Little Chalfont, Buckinghamshire, England) .. Yikon Genomics Single Cell Whole Genome Amplification Kit

Article Title: The microbiome of uncontacted Amerindians
Article Snippet: .. MDA of metagenomic DNA We performed MDA on fecal and oral metagenomic DNA from Amerindian subjects 3, 5, 6, and 23 and the five Puerto Rican subjects with the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. We pooled three to six reactions per sample to reduce MDA template amplification bias.

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen REPLI-g Single Cell Kit (MDA-2, Qiagen, Düsseldorf, Germany); GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3, GE Healthcare, Little Chalfont, Buckinghamshire, England); and Yikon Genomics Single Cell Whole Genome Amplification Kit (MALBAC, Yikon Genomics, China). ..

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits used were Qiagen REPLI-g Mini Kit (MDA-1), Qiagen REPLI-g Single Cell Kit (MDA-2), GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3), GenomePlex® Single Cell WGA Kit (DOP-1), Silicon Biosystem Ampli1™ WGA Kit (DOP-2), NEB Single Cell WGA Kit (DOP-3), and Yikon Genomics Single Cell WGA Kit (MALBAC). .. Library construction and whole-genome DNA sequencing The Illumina sequencer and LifeTech Ion Proton sequencer were used as the sequencing platforms in this study.

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates
Article Snippet: .. For low gDNA input (15 cells), the highest reproducibility was achieved for Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA) followed by the Single Cell WGA Kit (QPLS-based. .. LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA : Single Primer Isothermal Amplification. (PDF 453 KB) Additional file 2: Table S1: Sex determination from the genotyping results.

Whole Genome Amplification:

Article Title: Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
Article Snippet: .. For the F1 family V . rupestris B38 x ‘Chardonnay’, whole genome amplification was performed using 10 ng of DNA and the Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare) to obtain 1.0 μg of dried DNA per sample. .. Then, 48-plex GBS library preparation and sequencing was completed, as described previously [ ].

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates
Article Snippet: .. Two widely used MDA-based WGA systems were tested, namely the Illustra GenomiPhi V2 DNA amplification kit (#25-6600-30, GE Healthcare Bio-Sciences Corp., QC, Canada) and the REPLI-g Mini Kit (#150023, Qiagen, Mississauga, ON, Canada). .. The QPLS technology is the principle of the Single Cell WGA Kit (#E2620, New England Biolab, Pickering, ON, Canada).

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen REPLI-g Single Cell Kit (MDA-2, Qiagen, Düsseldorf, Germany); GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3, GE Healthcare, Little Chalfont, Buckinghamshire, England); and Yikon Genomics Single Cell Whole Genome Amplification Kit (MALBAC, Yikon Genomics, China). ..

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits used were Qiagen REPLI-g Mini Kit (MDA-1), Qiagen REPLI-g Single Cell Kit (MDA-2), GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3), GenomePlex® Single Cell WGA Kit (DOP-1), Silicon Biosystem Ampli1™ WGA Kit (DOP-2), NEB Single Cell WGA Kit (DOP-3), and Yikon Genomics Single Cell WGA Kit (MALBAC). .. Library construction and whole-genome DNA sequencing The Illumina sequencer and LifeTech Ion Proton sequencer were used as the sequencing platforms in this study.

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates
Article Snippet: .. For low gDNA input (15 cells), the highest reproducibility was achieved for Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA) followed by the Single Cell WGA Kit (QPLS-based. .. LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA : Single Primer Isothermal Amplification. (PDF 453 KB) Additional file 2: Table S1: Sex determination from the genotyping results.

Multiple Displacement Amplification:

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates
Article Snippet: .. Two widely used MDA-based WGA systems were tested, namely the Illustra GenomiPhi V2 DNA amplification kit (#25-6600-30, GE Healthcare Bio-Sciences Corp., QC, Canada) and the REPLI-g Mini Kit (#150023, Qiagen, Mississauga, ON, Canada). .. The QPLS technology is the principle of the Single Cell WGA Kit (#E2620, New England Biolab, Pickering, ON, Canada).

Article Title: The microbiome of uncontacted Amerindians
Article Snippet: .. MDA of metagenomic DNA We performed MDA on fecal and oral metagenomic DNA from Amerindian subjects 3, 5, 6, and 23 and the five Puerto Rican subjects with the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences). .. We pooled three to six reactions per sample to reduce MDA template amplification bias.

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen REPLI-g Single Cell Kit (MDA-2, Qiagen, Düsseldorf, Germany); GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3, GE Healthcare, Little Chalfont, Buckinghamshire, England); and Yikon Genomics Single Cell Whole Genome Amplification Kit (MALBAC, Yikon Genomics, China). ..

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits used were Qiagen REPLI-g Mini Kit (MDA-1), Qiagen REPLI-g Single Cell Kit (MDA-2), GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3), GenomePlex® Single Cell WGA Kit (DOP-1), Silicon Biosystem Ampli1™ WGA Kit (DOP-2), NEB Single Cell WGA Kit (DOP-3), and Yikon Genomics Single Cell WGA Kit (MALBAC). .. Library construction and whole-genome DNA sequencing The Illumina sequencer and LifeTech Ion Proton sequencer were used as the sequencing platforms in this study.

Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates
Article Snippet: .. For low gDNA input (15 cells), the highest reproducibility was achieved for Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA) followed by the Single Cell WGA Kit (QPLS-based. .. LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA : Single Primer Isothermal Amplification. (PDF 453 KB) Additional file 2: Table S1: Sex determination from the genotyping results.

Multiple Annealing and Looping–Based Amplification Cycles:

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen REPLI-g Single Cell Kit (MDA-2, Qiagen, Düsseldorf, Germany); GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3, GE Healthcare, Little Chalfont, Buckinghamshire, England); and Yikon Genomics Single Cell Whole Genome Amplification Kit (MALBAC, Yikon Genomics, China). ..

Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
Article Snippet: .. The kits used were Qiagen REPLI-g Mini Kit (MDA-1), Qiagen REPLI-g Single Cell Kit (MDA-2), GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3), GenomePlex® Single Cell WGA Kit (DOP-1), Silicon Biosystem Ampli1™ WGA Kit (DOP-2), NEB Single Cell WGA Kit (DOP-3), and Yikon Genomics Single Cell WGA Kit (MALBAC). .. Library construction and whole-genome DNA sequencing The Illumina sequencer and LifeTech Ion Proton sequencer were used as the sequencing platforms in this study.

Purification:

Article Title: Involvement of condensin-directed gene associations in the organization and regulation of chromosome territories during the cell cycle
Article Snippet: .. The respective chromosomes were purified using Qiaquick gel extraction Kit (Qiagen) and amplified using Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare). .. The chromosome-specific FISH probes were also generated using polymerase chain reaction (PCR)-amplified DNA fragments.

Gel Extraction:

Article Title: Involvement of condensin-directed gene associations in the organization and regulation of chromosome territories during the cell cycle
Article Snippet: .. The respective chromosomes were purified using Qiaquick gel extraction Kit (Qiagen) and amplified using Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare). .. The chromosome-specific FISH probes were also generated using polymerase chain reaction (PCR)-amplified DNA fragments.

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    GE Healthcare illustra genomiphi v2 dna amplification kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Illustra Genomiphi V2 Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 dna amplification kit/product/GE Healthcare
    Average 93 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 dna amplification kit - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Journal: GigaScience

    Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

    doi: 10.1186/s13742-015-0068-3

    Figure Lengend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Article Snippet: The kits used were Qiagen REPLI-g Mini Kit (MDA-1), Qiagen REPLI-g Single Cell Kit (MDA-2), GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3), GenomePlex® Single Cell WGA Kit (DOP-1), Silicon Biosystem Ampli1™ WGA Kit (DOP-2), NEB Single Cell WGA Kit (DOP-3), and Yikon Genomics Single Cell WGA Kit (MALBAC).

    Techniques: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

    Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

    Journal: BMC Genomics

    Article Title: Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates

    doi: 10.1186/1471-2164-15-889

    Figure Lengend Snippet: Density plot quality scores for erroneous and correct genotypes. The MDA row represents the results obtained from the Illustra GenomiPhi V2 DNA amplification kit (MDA-based WGA). LMA: Ligation-Mediated Amplification; MDA: Multiple Displacement Amplification; QPLS: Quasi-random Primed Library Synthesis followed by PCR amplification; SPIA: Single Primer Isothermal Amplification.

    Article Snippet: Two widely used MDA-based WGA systems were tested, namely the Illustra GenomiPhi V2 DNA amplification kit (#25-6600-30, GE Healthcare Bio-Sciences Corp., QC, Canada) and the REPLI-g Mini Kit (#150023, Qiagen, Mississauga, ON, Canada).

    Techniques: Multiple Displacement Amplification, Amplification, Whole Genome Amplification, Ligation, Random Primed, Polymerase Chain Reaction