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Illumina Inc illumina multiplexing oligos
Illumina Multiplexing Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illumina multiplexing oligos/product/Illumina Inc
Average 85 stars, based on 1 article reviews
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illumina multiplexing oligos - by Bioz Stars, 2020-07
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Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Epistasis dominates the genetic architecture of Drosophila quantitative traits
Article Snippet: .. Libraries were barcoded using Illumina Multiplexing oligos (Illumina), enabling two to be sequenced per HiSEq. ..

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  • 94
    Illumina Inc illumina adapters
    Schematic representation of different strategies for viral genome resequencing. A) Total RNA library: all the RNA species present in the sample are sequenced, no assumption on which genome is present, B) Hybridisation capture of a mRNA library: a good reference genome is needed to design the probes for capture, C) PCR enrichment: the desired genome is amplified from cDNA, a reference genome is needed to design specific oligos. Red lines, genomes of interest; Blue segments, <t>Illumina</t> adapters; Black lines, other RNA species.
    Illumina Adapters, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina adapters/product/Illumina Inc
    Average 94 stars, based on 781 article reviews
    Price from $9.99 to $1999.99
    illumina adapters - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    99
    Illumina Inc illumina miseq based sequencing
    Preparation and sequencing of samples. (A) Plasma is isolated from whole blood from up to 96 samples. (B) Viral RNA is isolated from up to 1 ml of plasma. (C) Viral RNA is used in a one-step RT-PCR amplification of a 2.8 kb region of the pol gene. When nested PCR is required, a 4.8 kb region is amplified as an external PCR followed by the 2.8 kb nested PCR of the pol gene. (D) PCR products are purified either by gel electrophoresis followed by gel extraction or through size-exclusion magnetic beads and then quantitated using the Qubit system. (E) Purified products are randomly fragmented and subjected to a limited cycle PCR to add sequencing adaptors and indices used for multiplexing samples. (F) Newly created libraries are purified by size-exclusion magnetic beads to remove short fragments. (G) The average size of the library fragments are calculated by bioanalysis and final concentration of the libraries calculated by Qubit are used to normalize each library and pool multiple libraries together at equimolar ratios. (H) Libraries are sequenced on the <t>Illumina</t> <t>MiSeq.</t> (I) Geneious Pro Software is used to trim sequencing reads based on quality scores and assemble the reads to a HXB2 reference sequence annotated with HIV drug resistance mutations. Geneious is used to identify variants within each sample relative to HXB2. Finally, variants associated with drug resistance mutations were extracted and their frequencies noted. Details about the analysis parameters are outlined in the Methods section.
    Illumina Miseq Based Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miseq based sequencing/product/Illumina Inc
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    illumina miseq based sequencing - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Illumina Inc multiplexing sequencing primers
    Preparation and sequencing of samples. (A) Plasma is isolated from whole blood from up to 96 samples. (B) Viral RNA is isolated from up to 1 ml of plasma. (C) Viral RNA is used in a one-step RT-PCR amplification of a 2.8 kb region of the pol gene. When nested PCR is required, a 4.8 kb region is amplified as an external PCR followed by the 2.8 kb nested PCR of the pol gene. (D) PCR products are purified either by gel electrophoresis followed by gel extraction or through size-exclusion magnetic beads and then quantitated using the Qubit system. (E) Purified products are randomly fragmented and subjected to a limited cycle PCR to add sequencing adaptors and indices used for multiplexing samples. (F) Newly created libraries are purified by size-exclusion magnetic beads to remove short fragments. (G) The average size of the library fragments are calculated by bioanalysis and final concentration of the libraries calculated by Qubit are used to normalize each library and pool multiple libraries together at equimolar ratios. (H) Libraries are sequenced on the <t>Illumina</t> <t>MiSeq.</t> (I) Geneious Pro Software is used to trim sequencing reads based on quality scores and assemble the reads to a HXB2 reference sequence annotated with HIV drug resistance mutations. Geneious is used to identify variants within each sample relative to HXB2. Finally, variants associated with drug resistance mutations were extracted and their frequencies noted. Details about the analysis parameters are outlined in the Methods section.
    Multiplexing Sequencing Primers, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplexing sequencing primers/product/Illumina Inc
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    multiplexing sequencing primers - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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    Schematic representation of different strategies for viral genome resequencing. A) Total RNA library: all the RNA species present in the sample are sequenced, no assumption on which genome is present, B) Hybridisation capture of a mRNA library: a good reference genome is needed to design the probes for capture, C) PCR enrichment: the desired genome is amplified from cDNA, a reference genome is needed to design specific oligos. Red lines, genomes of interest; Blue segments, Illumina adapters; Black lines, other RNA species.

    Journal: PLoS ONE

    Article Title: A Modified RNA-Seq Approach for Whole Genome Sequencing of RNA Viruses from Faecal and Blood Samples

    doi: 10.1371/journal.pone.0066129

    Figure Lengend Snippet: Schematic representation of different strategies for viral genome resequencing. A) Total RNA library: all the RNA species present in the sample are sequenced, no assumption on which genome is present, B) Hybridisation capture of a mRNA library: a good reference genome is needed to design the probes for capture, C) PCR enrichment: the desired genome is amplified from cDNA, a reference genome is needed to design specific oligos. Red lines, genomes of interest; Blue segments, Illumina adapters; Black lines, other RNA species.

    Article Snippet: Additionally, upon ligation of Illumina Adapters (Multiplexing Sample Preparation Oligonucleotide Kit) each library was size selected with two Ampure Bead steps (firstly, 1∶0.7× volume and secondly, the supernatant from the first bind was taken for a 1∶1.7× volume clean-up), selecting 200–600 bp fragments in 30 µl 10 mM Tris-Cl, pH 8.5.

    Techniques: Hybridization, Capture-C, Polymerase Chain Reaction, Amplification

    Parent-of-Origin cis eQTLs, Related to Table 1 Each of the 28 panels shows a chromosome scan for additive parent of origin effects of an Illumina microarray probe across the chromosome containing the cognate gene. The title of each panel gives the probe name, the gene symbol and the chromosome. The x axis is chromosome position in Mb. The y axis is the negative log10 p value of the test for an additive parent of origin effect in a mixed model (i.e., from a comparison between the fit of an additive nonparent of origin QTL versus an additive parent of origin QTL). The black lines are the chromosome scan logP values. The red vertical line within each panel is the position of the gene.

    Journal: Cell

    Article Title: The Architecture of Parent-of-Origin Effects in Mice

    doi: 10.1016/j.cell.2013.11.043

    Figure Lengend Snippet: Parent-of-Origin cis eQTLs, Related to Table 1 Each of the 28 panels shows a chromosome scan for additive parent of origin effects of an Illumina microarray probe across the chromosome containing the cognate gene. The title of each panel gives the probe name, the gene symbol and the chromosome. The x axis is chromosome position in Mb. The y axis is the negative log10 p value of the test for an additive parent of origin effect in a mixed model (i.e., from a comparison between the fit of an additive nonparent of origin QTL versus an additive parent of origin QTL). The black lines are the chromosome scan logP values. The red vertical line within each panel is the position of the gene.

    Article Snippet: Upon ligation of Illumina Adapters (Multiplexing Sample Preparation Oligonucleotide Kit) each library was size selected with two Ampure Bead bindings (first, 1:0.7x volume and second, the supernatant from the first bind was taken for a 1:1.7x volume clean-up).

    Techniques: Microarray

    Preparation and sequencing of samples. (A) Plasma is isolated from whole blood from up to 96 samples. (B) Viral RNA is isolated from up to 1 ml of plasma. (C) Viral RNA is used in a one-step RT-PCR amplification of a 2.8 kb region of the pol gene. When nested PCR is required, a 4.8 kb region is amplified as an external PCR followed by the 2.8 kb nested PCR of the pol gene. (D) PCR products are purified either by gel electrophoresis followed by gel extraction or through size-exclusion magnetic beads and then quantitated using the Qubit system. (E) Purified products are randomly fragmented and subjected to a limited cycle PCR to add sequencing adaptors and indices used for multiplexing samples. (F) Newly created libraries are purified by size-exclusion magnetic beads to remove short fragments. (G) The average size of the library fragments are calculated by bioanalysis and final concentration of the libraries calculated by Qubit are used to normalize each library and pool multiple libraries together at equimolar ratios. (H) Libraries are sequenced on the Illumina MiSeq. (I) Geneious Pro Software is used to trim sequencing reads based on quality scores and assemble the reads to a HXB2 reference sequence annotated with HIV drug resistance mutations. Geneious is used to identify variants within each sample relative to HXB2. Finally, variants associated with drug resistance mutations were extracted and their frequencies noted. Details about the analysis parameters are outlined in the Methods section.

    Journal: Retrovirology

    Article Title: Cross-clade simultaneous HIV drug resistance genotyping for reverse transcriptase, protease, and integrase inhibitor mutations by Illumina MiSeq

    doi: 10.1186/s12977-014-0122-8

    Figure Lengend Snippet: Preparation and sequencing of samples. (A) Plasma is isolated from whole blood from up to 96 samples. (B) Viral RNA is isolated from up to 1 ml of plasma. (C) Viral RNA is used in a one-step RT-PCR amplification of a 2.8 kb region of the pol gene. When nested PCR is required, a 4.8 kb region is amplified as an external PCR followed by the 2.8 kb nested PCR of the pol gene. (D) PCR products are purified either by gel electrophoresis followed by gel extraction or through size-exclusion magnetic beads and then quantitated using the Qubit system. (E) Purified products are randomly fragmented and subjected to a limited cycle PCR to add sequencing adaptors and indices used for multiplexing samples. (F) Newly created libraries are purified by size-exclusion magnetic beads to remove short fragments. (G) The average size of the library fragments are calculated by bioanalysis and final concentration of the libraries calculated by Qubit are used to normalize each library and pool multiple libraries together at equimolar ratios. (H) Libraries are sequenced on the Illumina MiSeq. (I) Geneious Pro Software is used to trim sequencing reads based on quality scores and assemble the reads to a HXB2 reference sequence annotated with HIV drug resistance mutations. Geneious is used to identify variants within each sample relative to HXB2. Finally, variants associated with drug resistance mutations were extracted and their frequencies noted. Details about the analysis parameters are outlined in the Methods section.

    Article Snippet: These primers were used in an Illumina MiSeq-based sequencing method for the detection of drug resistance in primary HIV samples from individuals failing treatment after release from prison.

    Techniques: Sequencing, Isolation, Reverse Transcription Polymerase Chain Reaction, Amplification, Nested PCR, Polymerase Chain Reaction, Purification, Nucleic Acid Electrophoresis, Gel Extraction, Magnetic Beads, Multiplexing, Concentration Assay, Software