il 17a  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    IL 17A Rat ELISA Kit
    Description:
    The rat IL 17A ELISA is an enzyme linked immunosorbent assay for the quantitative detection of rat IL 17A IL 17A a homodimeric cytokine of about 32 kDa is largely produced by activated memory T lymphocytes but stimulates innate immunity and host defense IL 17A and IL 17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction as well as increased survival locally IL 17A and IL 17F production by T lymphocytes is regulated by IL 23 independent of T cell receptor activation The Th1 and Th2 cell classification has until recently provided the framework for understanding CD4 T cell biology and the interplay between innate and adaptive immunity The Th17 lineage produces interleukin 17 which is highly proinflammatory and induces severe autoimmunity Increased levels of IL 17 have been associated with several conditions including airway inflammation rheumatoid arthritis intraperitoneal abscesses and adhesions inflammatory bowel disease allograft rejection psoriasis cancer and multiple sclerosis ConjugateBiotinSuitable Sample Typescell culture supernatant serum plasma EDTA citrate heparin Sample Volume50 µLReported ApplicationELISA
    Catalog Number:
    BMS635
    Price:
    None
    Category:
    Kits and Assays
    Applications:
    Protein Assays and Analysis|Protein Biology
    Buy from Supplier


    Structured Review

    Thermo Fisher il 17a
    Expression of inflammatory factors in colonic mucosa in mice with colitis. (A) interleukin (IL)-4, (B) IL-9, (C) <t>IL-17A,</t> and (D) IL-1β. Data are presented as means ± SEM ( n = 8). * p
    The rat IL 17A ELISA is an enzyme linked immunosorbent assay for the quantitative detection of rat IL 17A IL 17A a homodimeric cytokine of about 32 kDa is largely produced by activated memory T lymphocytes but stimulates innate immunity and host defense IL 17A and IL 17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction as well as increased survival locally IL 17A and IL 17F production by T lymphocytes is regulated by IL 23 independent of T cell receptor activation The Th1 and Th2 cell classification has until recently provided the framework for understanding CD4 T cell biology and the interplay between innate and adaptive immunity The Th17 lineage produces interleukin 17 which is highly proinflammatory and induces severe autoimmunity Increased levels of IL 17 have been associated with several conditions including airway inflammation rheumatoid arthritis intraperitoneal abscesses and adhesions inflammatory bowel disease allograft rejection psoriasis cancer and multiple sclerosis ConjugateBiotinSuitable Sample Typescell culture supernatant serum plasma EDTA citrate heparin Sample Volume50 µLReported ApplicationELISA
    https://www.bioz.com/result/il 17a/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 17a - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Sishen Pill Treatment of DSS-Induced Colitis via Regulating Interaction With Inflammatory Dendritic Cells and Gut Microbiota"

    Article Title: Sishen Pill Treatment of DSS-Induced Colitis via Regulating Interaction With Inflammatory Dendritic Cells and Gut Microbiota

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00801

    Expression of inflammatory factors in colonic mucosa in mice with colitis. (A) interleukin (IL)-4, (B) IL-9, (C) IL-17A, and (D) IL-1β. Data are presented as means ± SEM ( n = 8). * p
    Figure Legend Snippet: Expression of inflammatory factors in colonic mucosa in mice with colitis. (A) interleukin (IL)-4, (B) IL-9, (C) IL-17A, and (D) IL-1β. Data are presented as means ± SEM ( n = 8). * p

    Techniques Used: Expressing, Mouse Assay

    2) Product Images from "AN INULIN-TYPE FRUCTAN ENRICHED EXCLUSIVE ENTERAL NUTRITION FORMULA SUPPRESSES COLITIS THROUGH GUT MICROBIOME MODULATION AND PROMOTING EXPANSION OF ANTI-INFLAMMATORY T CELL SUBSETS"

    Article Title: AN INULIN-TYPE FRUCTAN ENRICHED EXCLUSIVE ENTERAL NUTRITION FORMULA SUPPRESSES COLITIS THROUGH GUT MICROBIOME MODULATION AND PROMOTING EXPANSION OF ANTI-INFLAMMATORY T CELL SUBSETS

    Journal: bioRxiv

    doi: 10.1101/2021.02.02.429403

    ( A ) Representative gating strategy for the enumeration of Foxp3 + IL-10 + CD4 + T cells. Proportions of Tbet + IFNγ + ([ B ] spleen and [ C ] MLN), Tbet + TNF + ([ D ] spleen and [ E ] MLN), Rorγt + IL-17A + ([ F ] spleen and [ G ] MLN), Rorγt + IL-22 + ([ H ] spleen and [ I ] MLN) and Foxp3 + IL-10 + ([ J ] spleen and [ K ] MLN) CD4 + T cells in mice from the chow, EEN and EEN-IN groups (n = 8 to 13 mice per group; each data point represents one mouse). * p
    Figure Legend Snippet: ( A ) Representative gating strategy for the enumeration of Foxp3 + IL-10 + CD4 + T cells. Proportions of Tbet + IFNγ + ([ B ] spleen and [ C ] MLN), Tbet + TNF + ([ D ] spleen and [ E ] MLN), Rorγt + IL-17A + ([ F ] spleen and [ G ] MLN), Rorγt + IL-22 + ([ H ] spleen and [ I ] MLN) and Foxp3 + IL-10 + ([ J ] spleen and [ K ] MLN) CD4 + T cells in mice from the chow, EEN and EEN-IN groups (n = 8 to 13 mice per group; each data point represents one mouse). * p

    Techniques Used: Mouse Assay

    3) Product Images from "IL-17A-Mediated Protection against Acanthamoeba Keratitis"

    Article Title: IL-17A-Mediated Protection against Acanthamoeba Keratitis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1302707

    Induction of Th1, Th2 and Th17 effector T cell response in the cornea and local dLNs after corneal Acanthamoeba infection C57BL/6J mice corneas were intrastromally injected with 2.5 × 10 4 Acanthamoebae. Flow cytometry was used to detect IFN-γ, IL-17A and IL-4 production by CD4 + T cells (anti-CD3/anti-CD28 stimulated for 5 hrs) obtained from pooled corneas on day 5 and day 8 pi. Representative FACS plots (left), and cell numbers (right) for IFN-γ (A) , IL-17A (B) and IL-4 (C) secreting CD4 + T cells from pooled corneas are shown. IFN-γ (D) , IL-4 (E) and IL-17A (F) protein levels in pooled corneal samples at indicated days pi. Data are representative of at least two independent experiments and show mean values ± SEM. Combined results of two separate experiments are shown, 5 to 6 corneas/group were used in each experiment. * p ≤0.05. Statistical levels of significance were analyzed by Student’s t test (unpaired). The total cell numbers of IFN-γ ( G ); IL-17A ( H ) and IL-4 ( I ) producing CD4 + T cells in dLN of uninfected (day 0) and Acanthamoeba infected (day 5 and day 8 pi) mice are shown. Data are representative of two independent experiments and show mean values ± SEM (n = 8 mice at each indicated time point p.i.). *** p ≤ 0.001,** p ≤ 0.01,* p ≤ 0.05, ns- non-significant. Statistical levels of significance were analyzed by one-way ANOVA test with Tukey’s multiple comparison test.
    Figure Legend Snippet: Induction of Th1, Th2 and Th17 effector T cell response in the cornea and local dLNs after corneal Acanthamoeba infection C57BL/6J mice corneas were intrastromally injected with 2.5 × 10 4 Acanthamoebae. Flow cytometry was used to detect IFN-γ, IL-17A and IL-4 production by CD4 + T cells (anti-CD3/anti-CD28 stimulated for 5 hrs) obtained from pooled corneas on day 5 and day 8 pi. Representative FACS plots (left), and cell numbers (right) for IFN-γ (A) , IL-17A (B) and IL-4 (C) secreting CD4 + T cells from pooled corneas are shown. IFN-γ (D) , IL-4 (E) and IL-17A (F) protein levels in pooled corneal samples at indicated days pi. Data are representative of at least two independent experiments and show mean values ± SEM. Combined results of two separate experiments are shown, 5 to 6 corneas/group were used in each experiment. * p ≤0.05. Statistical levels of significance were analyzed by Student’s t test (unpaired). The total cell numbers of IFN-γ ( G ); IL-17A ( H ) and IL-4 ( I ) producing CD4 + T cells in dLN of uninfected (day 0) and Acanthamoeba infected (day 5 and day 8 pi) mice are shown. Data are representative of two independent experiments and show mean values ± SEM (n = 8 mice at each indicated time point p.i.). *** p ≤ 0.001,** p ≤ 0.01,* p ≤ 0.05, ns- non-significant. Statistical levels of significance were analyzed by one-way ANOVA test with Tukey’s multiple comparison test.

    Techniques Used: Infection, Mouse Assay, Injection, Flow Cytometry, Cytometry, FACS

    IL-17A neutralization post Acanthamoeba infection exacerbates keratitis severity C57BL/6J mice corneas were intrastromally injected with 2.5 × 10 4 Acanthamoebae for corneal infection. Infected mice were treated with anti-IL-17A (5 μg in 10 μl/eye) or isotype mAb (5 μg in 10 μl/eye) by local subconjunctival injections from day 1 to 7 pi. The progression of keratitis was monitored by measuring corneal opacity scores on day 1, 3, 5 and 7 pi. (A) The severity of Acanthamoeba keratitis was significantly increased in anti-IL-17A-treated animals compared to control mice treated with isotype mAb. Kinetic analysis for mean scores at each indicated day pi is shown. (B) Individual eye scores for corneal opacity on day 7 pi from anti-IL-17A and isotype control groups are shown. (C) Representative eye photos on day 1, 3, 5 and 7 pi show corneal lesion severity from anti-IL-17A-treated animals as compared to isotype mAb-treated mice. All eye pictures at different days pi are from the same eye of each group. (D) Total number of RFP + Acanthamoebae on day 3 pi between anti-IL-17A and isotype mAb treated group. (E) Total number of neutrophils on day 3 pi between anti-IL-17A and isotype mAb treated group. Data are representative of two independent experiments and show mean values ± SEM (n = 22–25 mice/group; * p
    Figure Legend Snippet: IL-17A neutralization post Acanthamoeba infection exacerbates keratitis severity C57BL/6J mice corneas were intrastromally injected with 2.5 × 10 4 Acanthamoebae for corneal infection. Infected mice were treated with anti-IL-17A (5 μg in 10 μl/eye) or isotype mAb (5 μg in 10 μl/eye) by local subconjunctival injections from day 1 to 7 pi. The progression of keratitis was monitored by measuring corneal opacity scores on day 1, 3, 5 and 7 pi. (A) The severity of Acanthamoeba keratitis was significantly increased in anti-IL-17A-treated animals compared to control mice treated with isotype mAb. Kinetic analysis for mean scores at each indicated day pi is shown. (B) Individual eye scores for corneal opacity on day 7 pi from anti-IL-17A and isotype control groups are shown. (C) Representative eye photos on day 1, 3, 5 and 7 pi show corneal lesion severity from anti-IL-17A-treated animals as compared to isotype mAb-treated mice. All eye pictures at different days pi are from the same eye of each group. (D) Total number of RFP + Acanthamoebae on day 3 pi between anti-IL-17A and isotype mAb treated group. (E) Total number of neutrophils on day 3 pi between anti-IL-17A and isotype mAb treated group. Data are representative of two independent experiments and show mean values ± SEM (n = 22–25 mice/group; * p

    Techniques Used: Neutralization, Infection, Mouse Assay, Injection

    IL-17A neutralization after corneal Acanthamoeba infection promotes CD4 + T cell effector and regulatory T cell response in lymphoid organs Acanthamoeba -infected C57BL/6J mice were treated with anti-IL-17A or isotype mAb by local subconjunctival injections from day 1 to 7 pi. Flow cytometry was used to detect IFN-γ, IL-17A and IL-4 production by CD4 + T cells (anti-CD3/anti-CD28 stimulated for 5 hrs) obtained from dLN of anti-IL-17A- and isotype-treated animals on day 8 pi. Representative FACS plots (left) and total cell numbers (right) of IFN-γ (A) , IL-17A (B) , IL-4 (C) producing CD4 + T cells and Foxp3 + CD4 + T cells (D) in dLN on day 8 pi are shown. Cell ratios for total numbers of Treg per Th1 cell (E) and Treg per Th17 (F) in dLN from anti-IL-17A- (clear bar) and isotype mAb-treated (grey bar) groups. Data are representative of two independent experiments and show mean values ± SEM (n = 8 mice at each indicated time point p.i.). *** p ≤ 0.001,** p ≤ 0.01,* p ≤ 0.05, ns- non-significant. Statistical levels of significance were analyzed by one-way ANOVA test with Tukey’s multiple comparison test.
    Figure Legend Snippet: IL-17A neutralization after corneal Acanthamoeba infection promotes CD4 + T cell effector and regulatory T cell response in lymphoid organs Acanthamoeba -infected C57BL/6J mice were treated with anti-IL-17A or isotype mAb by local subconjunctival injections from day 1 to 7 pi. Flow cytometry was used to detect IFN-γ, IL-17A and IL-4 production by CD4 + T cells (anti-CD3/anti-CD28 stimulated for 5 hrs) obtained from dLN of anti-IL-17A- and isotype-treated animals on day 8 pi. Representative FACS plots (left) and total cell numbers (right) of IFN-γ (A) , IL-17A (B) , IL-4 (C) producing CD4 + T cells and Foxp3 + CD4 + T cells (D) in dLN on day 8 pi are shown. Cell ratios for total numbers of Treg per Th1 cell (E) and Treg per Th17 (F) in dLN from anti-IL-17A- (clear bar) and isotype mAb-treated (grey bar) groups. Data are representative of two independent experiments and show mean values ± SEM (n = 8 mice at each indicated time point p.i.). *** p ≤ 0.001,** p ≤ 0.01,* p ≤ 0.05, ns- non-significant. Statistical levels of significance were analyzed by one-way ANOVA test with Tukey’s multiple comparison test.

    Techniques Used: Neutralization, Infection, Mouse Assay, Flow Cytometry, Cytometry, FACS

    IL-17A neutralization after corneal Acanthamoeba infection promotes migration of both innate and adaptive immune cells in the cornea Acanthamoeba -infected C57BL/6J mice were treated with anti-IL-17A or isotype mAb by local subconjunctival injections from day 1 to 7 pi. Immune cells infiltrating the corneas were quantified on day 8 pi by flow cytometry. Representative FACS plots (left) and total cell numbers (right) of CD45 + cells ( A ), CD11b + Ly6G + polymorphonuclear neutrophils gated on CD45 + cells ( B ) and, CD4 + T cells gated on CD45 + cells ( C). Representative FACS plots (left) and total cell numbers (right) of IFN-γ (D) and IL-17A (E) producing CD4 + T cells harvested from the corneas on day 8 pi and stimulated with anti-CD3/anti-CD28 for 5 h. (F) Representative FACS plots (left) and total cell numbers (right) of Foxp3 + CD4 + T cells. Cell ratios for total numbers of Treg per Th1 cell (G) and Treg per Th17 (H) in the cornea from anti-IL-17A- (clear bar) and isotype mAb-treated (grey bar) groups. Results of a representative experiment of two with a minimum 8 mice per group are shown.
    Figure Legend Snippet: IL-17A neutralization after corneal Acanthamoeba infection promotes migration of both innate and adaptive immune cells in the cornea Acanthamoeba -infected C57BL/6J mice were treated with anti-IL-17A or isotype mAb by local subconjunctival injections from day 1 to 7 pi. Immune cells infiltrating the corneas were quantified on day 8 pi by flow cytometry. Representative FACS plots (left) and total cell numbers (right) of CD45 + cells ( A ), CD11b + Ly6G + polymorphonuclear neutrophils gated on CD45 + cells ( B ) and, CD4 + T cells gated on CD45 + cells ( C). Representative FACS plots (left) and total cell numbers (right) of IFN-γ (D) and IL-17A (E) producing CD4 + T cells harvested from the corneas on day 8 pi and stimulated with anti-CD3/anti-CD28 for 5 h. (F) Representative FACS plots (left) and total cell numbers (right) of Foxp3 + CD4 + T cells. Cell ratios for total numbers of Treg per Th1 cell (G) and Treg per Th17 (H) in the cornea from anti-IL-17A- (clear bar) and isotype mAb-treated (grey bar) groups. Results of a representative experiment of two with a minimum 8 mice per group are shown.

    Techniques Used: Neutralization, Infection, Migration, Mouse Assay, Flow Cytometry, Cytometry, FACS

    4) Product Images from "Vibrio vulnificus RtxA Is a Major Factor Driving Inflammatory T Helper Type 17 Cell Responses in vitro and in vivo"

    Article Title: Vibrio vulnificus RtxA Is a Major Factor Driving Inflammatory T Helper Type 17 Cell Responses in vitro and in vivo

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02095

    Vibrio vulnificus rtxA mutant was defective in inducing Th17 cell responses in vitro and in vivo . DCs were infected with WT and rtxA mutant V. vulnificus at an MOI of 10 for 60 min. After 20 h, DCs were co-cultured with naïve CD4 + T cells isolated from lymph nodes for 3 days in the presence of anti-CD3ε and anti-CD28 mAbs, followed by flow cytometric analysis of CD4 and IL-17 expression (A) or ELISA for IL-17A levels in the supernatants (B) . (C) The expression of CD4 and IL-17 in the lamina propria samples of uninfected mice or mice infected with WT and rtxA mutant (1 × 10 7 CFU per mouse) were analyzed by flow cytometry. The data shown in (A,C) are representative of three independent experiments, and bar graphs represent the means ± SD of three independent experiments. Bars and error bars in (B) represent the mean ± SD of results performed in triplicate. * p
    Figure Legend Snippet: Vibrio vulnificus rtxA mutant was defective in inducing Th17 cell responses in vitro and in vivo . DCs were infected with WT and rtxA mutant V. vulnificus at an MOI of 10 for 60 min. After 20 h, DCs were co-cultured with naïve CD4 + T cells isolated from lymph nodes for 3 days in the presence of anti-CD3ε and anti-CD28 mAbs, followed by flow cytometric analysis of CD4 and IL-17 expression (A) or ELISA for IL-17A levels in the supernatants (B) . (C) The expression of CD4 and IL-17 in the lamina propria samples of uninfected mice or mice infected with WT and rtxA mutant (1 × 10 7 CFU per mouse) were analyzed by flow cytometry. The data shown in (A,C) are representative of three independent experiments, and bar graphs represent the means ± SD of three independent experiments. Bars and error bars in (B) represent the mean ± SD of results performed in triplicate. * p

    Techniques Used: Mutagenesis, In Vitro, In Vivo, Infection, Cell Culture, Isolation, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Mouse Assay, Cytometry

    5) Product Images from "IL-17A secretion by CD8+ T cells supports Th17-mediated autoimmune encephalomyelitis"

    Article Title: IL-17A secretion by CD8+ T cells supports Th17-mediated autoimmune encephalomyelitis

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI63681

    IL-17A competence of CD4 + T cells is not required for their pathogenicity.
    Figure Legend Snippet: IL-17A competence of CD4 + T cells is not required for their pathogenicity.

    Techniques Used:

    IL-17A competence of CD8 + T cells accelerates CD4 + T cell encephalitogenicity.
    Figure Legend Snippet: IL-17A competence of CD8 + T cells accelerates CD4 + T cell encephalitogenicity.

    Techniques Used:

    6) Product Images from "IL-9 Inhibits Viral Replication in Coxsackievirus B3-Induced Myocarditis"

    Article Title: IL-9 Inhibits Viral Replication in Coxsackievirus B3-Induced Myocarditis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2016.00409

    IL-9 regulated serum IL-17a and TGF-β expressions in VMC mice . The levels of serum IL-9, IL-17a, IL-10, TGF-β, TNF-α, IFN-γ, IFN-α, and IFN-β in Control, WT, IL-9KO, and rIL-9 groups. * P
    Figure Legend Snippet: IL-9 regulated serum IL-17a and TGF-β expressions in VMC mice . The levels of serum IL-9, IL-17a, IL-10, TGF-β, TNF-α, IFN-γ, IFN-α, and IFN-β in Control, WT, IL-9KO, and rIL-9 groups. * P

    Techniques Used: Mouse Assay

    7) Product Images from "TLR2 Mediates Helicobacter pylori-Induced Tolerogenic Immune Response in Mice"

    Article Title: TLR2 Mediates Helicobacter pylori-Induced Tolerogenic Immune Response in Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0074595

    Enhanced H. pylori –specific Th1 response in chronically infected TLR2KO mice. BMDCs from WT mice were cultured. On day 6, 1 × 10 6 cells/mL of BMDCs were treated with H . pylori (H. pylori to DC ratio was 10 to 1). DCs were prepared 18 h after infection with H . pylori and cocultured with splenocytes from WT mice or TLR2KO mice after H . pylori infection of 2 months duration. During the 72 h coculture, the splenocyte-to-DC ratio was 10 to 1. The percentages of IFN-γ, IL-17A, and Foxp3 from splenocytes were determined using an intracellular cytokine staining assay. Representative flow plots demonstrate the increase of IFN-γ and the decrease of IL-17A and Foxp3 in TLR2KO splenocytes compared to WT splenocytes with gating held constant. At the same time, results are expressed as the mean of three sample replicates. A higher population of IFN-γ–positive cells are seen in TLR2KO splenocytes stimulated by H . pylori . In contrast, a lower population of IL-17A and Foxp3 cells are seen in TLR2KO splenocytes after H . pylori infection. Data represent means ± SEM, n = 5 in duplicate.
    Figure Legend Snippet: Enhanced H. pylori –specific Th1 response in chronically infected TLR2KO mice. BMDCs from WT mice were cultured. On day 6, 1 × 10 6 cells/mL of BMDCs were treated with H . pylori (H. pylori to DC ratio was 10 to 1). DCs were prepared 18 h after infection with H . pylori and cocultured with splenocytes from WT mice or TLR2KO mice after H . pylori infection of 2 months duration. During the 72 h coculture, the splenocyte-to-DC ratio was 10 to 1. The percentages of IFN-γ, IL-17A, and Foxp3 from splenocytes were determined using an intracellular cytokine staining assay. Representative flow plots demonstrate the increase of IFN-γ and the decrease of IL-17A and Foxp3 in TLR2KO splenocytes compared to WT splenocytes with gating held constant. At the same time, results are expressed as the mean of three sample replicates. A higher population of IFN-γ–positive cells are seen in TLR2KO splenocytes stimulated by H . pylori . In contrast, a lower population of IL-17A and Foxp3 cells are seen in TLR2KO splenocytes after H . pylori infection. Data represent means ± SEM, n = 5 in duplicate.

    Techniques Used: Infection, Mouse Assay, Cell Culture, Staining, Flow Cytometry

    Reduced Th17/Treg and increased Th1 responses in H. pylori –infected TLR2KO mice. After an H . pylori (10 9 CFU/mL) infection of 2 months duration, stomachs from WT mice and TLR2-deficient mice were removed. mRNA expression of Foxp3 ( a ), IFN-γ ( b ), IL-10 ( c ), IL-17A ( d ), TNF-α ( e ) and the housekeeping gene GAPDH were measured by quantitative PCR (n = 10 mice per group; * P
    Figure Legend Snippet: Reduced Th17/Treg and increased Th1 responses in H. pylori –infected TLR2KO mice. After an H . pylori (10 9 CFU/mL) infection of 2 months duration, stomachs from WT mice and TLR2-deficient mice were removed. mRNA expression of Foxp3 ( a ), IFN-γ ( b ), IL-10 ( c ), IL-17A ( d ), TNF-α ( e ) and the housekeeping gene GAPDH were measured by quantitative PCR (n = 10 mice per group; * P

    Techniques Used: Infection, Mouse Assay, Expressing, Real-time Polymerase Chain Reaction

    H. pylori –stimulated TLR2KO BMDCs induced a skewed Th1 response. BMDCs from WT mice and TLR2KO mice were stimulated with a blank control, live H . pylori (HP-L), or H . pylori sonicate (HP-S). DCs (1 × 10 5 cells/well) were prepared 18 h after H . pylori infection and cocultured with naive syngeneic splenocytes (1 × 10 6 cells/well) for 72 h at a splenocyte-to-DC ratio (10:1). Supernatants were collected and protein expression was measured by ELISA. The secretion of IFN-γ ( a ) was higher, whereas the secretion of IL-10 ( b ) and IL-17A ( c ) was lower in TLR2KO mice than in WT mice ( * P
    Figure Legend Snippet: H. pylori –stimulated TLR2KO BMDCs induced a skewed Th1 response. BMDCs from WT mice and TLR2KO mice were stimulated with a blank control, live H . pylori (HP-L), or H . pylori sonicate (HP-S). DCs (1 × 10 5 cells/well) were prepared 18 h after H . pylori infection and cocultured with naive syngeneic splenocytes (1 × 10 6 cells/well) for 72 h at a splenocyte-to-DC ratio (10:1). Supernatants were collected and protein expression was measured by ELISA. The secretion of IFN-γ ( a ) was higher, whereas the secretion of IL-10 ( b ) and IL-17A ( c ) was lower in TLR2KO mice than in WT mice ( * P

    Techniques Used: Mouse Assay, Infection, Expressing, Enzyme-linked Immunosorbent Assay

    8) Product Images from "Mycobacterium tuberculosis GroEL2 Modulates Dendritic Cell Responses"

    Article Title: Mycobacterium tuberculosis GroEL2 Modulates Dendritic Cell Responses

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00387-17

    GroEL2 proteolysis modulates DC antigen presentation and T cell polarization. (A) We pulsed DCs with various amounts of the OVA 323–339 peptide (1 μg/ml, 10 μg/ml, and 50 μg/ml) for 6 h and then stimulated cells with either recombinant GroEL2 or GroEL2(cl) for 24 h. Following coculture with antigen-specific TCR-Tg CD4 + T cells for 72 h, we assayed cells for the cytokines IFN-γ, IL-2, and IL-17A by an ELISA. (B) We infected DCs with live M. tuberculosis strains and then cocultured them with purified ESAT-6-specific TCR-Tg CD4 + T cells. After 80 h, cell-free supernatants were collected and assessed for the cytokines IFN-γ, IL-2, and IL-17A by an ELISA. Data are shown as means ± SD of results of one representative experiment from three independent experiments.
    Figure Legend Snippet: GroEL2 proteolysis modulates DC antigen presentation and T cell polarization. (A) We pulsed DCs with various amounts of the OVA 323–339 peptide (1 μg/ml, 10 μg/ml, and 50 μg/ml) for 6 h and then stimulated cells with either recombinant GroEL2 or GroEL2(cl) for 24 h. Following coculture with antigen-specific TCR-Tg CD4 + T cells for 72 h, we assayed cells for the cytokines IFN-γ, IL-2, and IL-17A by an ELISA. (B) We infected DCs with live M. tuberculosis strains and then cocultured them with purified ESAT-6-specific TCR-Tg CD4 + T cells. After 80 h, cell-free supernatants were collected and assessed for the cytokines IFN-γ, IL-2, and IL-17A by an ELISA. Data are shown as means ± SD of results of one representative experiment from three independent experiments.

    Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay, Infection, Purification

    9) Product Images from "Regulatory T Cell Induction during Plasmodium chabaudi Infection Modifies the Clinical Course of Experimental Autoimmune Encephalomyelitis"

    Article Title: Regulatory T Cell Induction during Plasmodium chabaudi Infection Modifies the Clinical Course of Experimental Autoimmune Encephalomyelitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0017849

    Induction of EAE in Plasmodium chabaudi -infected mice modulates their cytokine expression profile. Expression levels of IL-17A, IL-10, IL-27 and TGF-β 1 measured by qRT-PCR in P. chabaudi infected 6 days after EAE induction (black bars) and the EAE alone (gray bars) group. The values represent the mean number of specific cytokine gene copies relative to GAPDH in three-five mice ± SD.
    Figure Legend Snippet: Induction of EAE in Plasmodium chabaudi -infected mice modulates their cytokine expression profile. Expression levels of IL-17A, IL-10, IL-27 and TGF-β 1 measured by qRT-PCR in P. chabaudi infected 6 days after EAE induction (black bars) and the EAE alone (gray bars) group. The values represent the mean number of specific cytokine gene copies relative to GAPDH in three-five mice ± SD.

    Techniques Used: Infection, Mouse Assay, Expressing, Quantitative RT-PCR

    10) Product Images from "Gr-1+CD11b+ Immature Myeloid Cells (IMC) Promote Resistance of Pro-Inflammatory T Cells to Suppression by Regulatory T Cells in Atherosclerotic Apo E- Deficient Mice"

    Article Title: Gr-1+CD11b+ Immature Myeloid Cells (IMC) Promote Resistance of Pro-Inflammatory T Cells to Suppression by Regulatory T Cells in Atherosclerotic Apo E- Deficient Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0108620

    Gr-1 + CD11b + cell-induced unresponsiveness of pro-inflammatory T cells to suppression is IL-6 dependent. (a), Gr-1 + CD11b + cells were isolated from the spleen of 20-week old Apo E −/− and C57BL/6 mice and EL-4 tumor-bearing mice. The mRNA expression of Arg 1, iNOS 2, TGF-β1 and IL-6 was measured by qRT-PCR. (b), CD4 + CD25 − T cells from C57BL/6 mice were cultured with or without Gr-1 + CD11b + cells and Treg cells as well as 20 µg/ml IL-6 antibody, T cell proliferation was determined by [3H] thymidine incorporation. Data are the mean ± SEM (n = 4) of one representative experiment. Atherosclerotic Apo E −/− mice were pre-treated i.p. with 100 µg RB6-8C5 mAb or Jak inhibitor tofacitinib (CP-690,550) 30 mg/kg twice a week, (c), Phosphorylation of stat1 and stat3 as well as Erk was detected by immunoblot. Shown is one representative experiment out of three. the serum levels of IL-6 (d), INF-γ (e), IL-17A (f) and TGF-β1 (g) were measured by ELISA. Data are the mean ± SEM (n = 4) of one representative experiment. Similar results were obtained in at least three independent experiments. n.s = not significant; *p
    Figure Legend Snippet: Gr-1 + CD11b + cell-induced unresponsiveness of pro-inflammatory T cells to suppression is IL-6 dependent. (a), Gr-1 + CD11b + cells were isolated from the spleen of 20-week old Apo E −/− and C57BL/6 mice and EL-4 tumor-bearing mice. The mRNA expression of Arg 1, iNOS 2, TGF-β1 and IL-6 was measured by qRT-PCR. (b), CD4 + CD25 − T cells from C57BL/6 mice were cultured with or without Gr-1 + CD11b + cells and Treg cells as well as 20 µg/ml IL-6 antibody, T cell proliferation was determined by [3H] thymidine incorporation. Data are the mean ± SEM (n = 4) of one representative experiment. Atherosclerotic Apo E −/− mice were pre-treated i.p. with 100 µg RB6-8C5 mAb or Jak inhibitor tofacitinib (CP-690,550) 30 mg/kg twice a week, (c), Phosphorylation of stat1 and stat3 as well as Erk was detected by immunoblot. Shown is one representative experiment out of three. the serum levels of IL-6 (d), INF-γ (e), IL-17A (f) and TGF-β1 (g) were measured by ELISA. Data are the mean ± SEM (n = 4) of one representative experiment. Similar results were obtained in at least three independent experiments. n.s = not significant; *p

    Techniques Used: Isolation, Mouse Assay, Expressing, Quantitative RT-PCR, Cell Culture, Enzyme-linked Immunosorbent Assay

    Resistance of pro-inflammatory T cells to suppression, instead of impaired Treg cells, contributes to the ongoing inflammatory response in atherosclerotic Apo E −/− mice. (a), 6×10 4 CD4 + CD25 − T cells from C57BL/6 mice were stimulated with 3 µg/ml CD3 and 1 µg/ml CD28, in the presence or not of indicated numbers of Treg from 20-week old Apo E −/− mice or age-matched C57BL/6 mice for 3 days. 1 µCi of [3H] thymidine was added in each well 18 hrs before harvest, T cell proliferation was determined by [3H] thymidine incorporation. Data are the mean ± SEM (n = 4) of one representative experiment out of three performed. CD4 + CD25 − T cells isolated from 20-week old Apo E −/− mice or age-matched C57BL/6 mice were cultured alone or with Treg cells isolated from 20-week old Apo E −/− mice at 3∶1 ratio, T cell proliferation was examined as shown in (b). The amounts of IFN-γ (c) and IL-17A (d) in supernatant were measured by ELISA (n = 4). Data are representative of three independent experiments. n.s = not significant; *p
    Figure Legend Snippet: Resistance of pro-inflammatory T cells to suppression, instead of impaired Treg cells, contributes to the ongoing inflammatory response in atherosclerotic Apo E −/− mice. (a), 6×10 4 CD4 + CD25 − T cells from C57BL/6 mice were stimulated with 3 µg/ml CD3 and 1 µg/ml CD28, in the presence or not of indicated numbers of Treg from 20-week old Apo E −/− mice or age-matched C57BL/6 mice for 3 days. 1 µCi of [3H] thymidine was added in each well 18 hrs before harvest, T cell proliferation was determined by [3H] thymidine incorporation. Data are the mean ± SEM (n = 4) of one representative experiment out of three performed. CD4 + CD25 − T cells isolated from 20-week old Apo E −/− mice or age-matched C57BL/6 mice were cultured alone or with Treg cells isolated from 20-week old Apo E −/− mice at 3∶1 ratio, T cell proliferation was examined as shown in (b). The amounts of IFN-γ (c) and IL-17A (d) in supernatant were measured by ELISA (n = 4). Data are representative of three independent experiments. n.s = not significant; *p

    Techniques Used: Mouse Assay, Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

    11) Product Images from "Increased Interleukin-35 suppresses peripheral CD14+ monocytes function in patients with Kawasaki disease"

    Article Title: Increased Interleukin-35 suppresses peripheral CD14+ monocytes function in patients with Kawasaki disease

    Journal: BMC Immunology

    doi: 10.1186/s12865-020-00348-x

    Recombinant interleukin (IL)-35 stimulation to CD14 + monocytes induced naïve CD4 + T cell activation in patients with Kawasaki disease ( n = 18). CD14 + monocytes and CD4 + T cells were purified from peripheral bloods of Kwasaki disease patients. CD14 + monocytes were stimulated with recombinant human IL-35 (50 ng/ml) and 1 × lipopolysaccharide for 24 h. Direct contact and indirect contact co-culture system was set up between 10 5 of CD14 + monocytes and 10 5 of autologous CD4 + T cells. In the last 12 h of co-culture, phorbol 12-myristate 13-acetate (50 ng/ml), ionomycin (1 μg/ml), and Brefeldin A (10 μg/ml) were added. Cells were harvested 48 h post co-culture, and were stained with anti-CD4, anti-interferon-γ (IFN-γ), and anti-IL-17A for flow cytometry analysis. The isotype control was used for separation of positive and negative cells of IFN-γ and IL-17A. Typical flow dots analyses for (a) CD4 + IFN-γ + Th1 cells and (b) CD4 + IL-17A + Th17 cells in direct contact and indirect contact co-culture systems. (c) CD4 + IFN-γ + Th1 and (d) CD4 + IL-17A + Th17 percentage was elevated in direct contact co-culture system when compared with in indirect contact co-culture system or in CD4 + T cell cultured anlone. However, there was no significant difference of (c) Th1 or (d) Th17 percentage between CD4 + T cell cultured alone and CD14 + /CD4 + indirect contact co-culture system. IL-35 stimulation to CD14 + monocytes down-regulated (c) Th1 and (d) Th17 percentage in direct contact co-culture system, but not in indirect contact co-culture system. Paired t test was used for comparison. Individual level of each subject was shown. The horizon line presented mean, and error bar presented standard deviation
    Figure Legend Snippet: Recombinant interleukin (IL)-35 stimulation to CD14 + monocytes induced naïve CD4 + T cell activation in patients with Kawasaki disease ( n = 18). CD14 + monocytes and CD4 + T cells were purified from peripheral bloods of Kwasaki disease patients. CD14 + monocytes were stimulated with recombinant human IL-35 (50 ng/ml) and 1 × lipopolysaccharide for 24 h. Direct contact and indirect contact co-culture system was set up between 10 5 of CD14 + monocytes and 10 5 of autologous CD4 + T cells. In the last 12 h of co-culture, phorbol 12-myristate 13-acetate (50 ng/ml), ionomycin (1 μg/ml), and Brefeldin A (10 μg/ml) were added. Cells were harvested 48 h post co-culture, and were stained with anti-CD4, anti-interferon-γ (IFN-γ), and anti-IL-17A for flow cytometry analysis. The isotype control was used for separation of positive and negative cells of IFN-γ and IL-17A. Typical flow dots analyses for (a) CD4 + IFN-γ + Th1 cells and (b) CD4 + IL-17A + Th17 cells in direct contact and indirect contact co-culture systems. (c) CD4 + IFN-γ + Th1 and (d) CD4 + IL-17A + Th17 percentage was elevated in direct contact co-culture system when compared with in indirect contact co-culture system or in CD4 + T cell cultured anlone. However, there was no significant difference of (c) Th1 or (d) Th17 percentage between CD4 + T cell cultured alone and CD14 + /CD4 + indirect contact co-culture system. IL-35 stimulation to CD14 + monocytes down-regulated (c) Th1 and (d) Th17 percentage in direct contact co-culture system, but not in indirect contact co-culture system. Paired t test was used for comparison. Individual level of each subject was shown. The horizon line presented mean, and error bar presented standard deviation

    Techniques Used: Recombinant, Activation Assay, Purification, Co-Culture Assay, Staining, Flow Cytometry, Cell Culture, Standard Deviation

    12) Product Images from "Serum Cytokines Th1, Th2, and Th17 Expression Profiling in Active Lupus Nephritis-IV: From a Southern Chinese Han Population"

    Article Title: Serum Cytokines Th1, Th2, and Th17 Expression Profiling in Active Lupus Nephritis-IV: From a Southern Chinese Han Population

    Journal: Mediators of Inflammation

    doi: 10.1155/2016/4927530

    Receiver operating characteristics (ROC) curve of serum cytokines for differential diagnosis of LN-IV from LN-III and LN-V. The area under ROC curve of the serum cytokines levels IL-18, IL-17A, IL-18/IL-4, and IL-17A/IL-4 ratios was calculated and the accuracy of using these serum cytokines levels in the diagnosis of LN-IV from LN-III and LN-V was analyzed (data in Table 3 ).
    Figure Legend Snippet: Receiver operating characteristics (ROC) curve of serum cytokines for differential diagnosis of LN-IV from LN-III and LN-V. The area under ROC curve of the serum cytokines levels IL-18, IL-17A, IL-18/IL-4, and IL-17A/IL-4 ratios was calculated and the accuracy of using these serum cytokines levels in the diagnosis of LN-IV from LN-III and LN-V was analyzed (data in Table 3 ).

    Techniques Used:

    Comparison of serum levels Th1 cytokines (IFN- γ , IL-18, and IL-12p70), Th2 cytokine (IL-4), and Th17 cytokines (IL-17A and IL-6) in the LN classes and healthy control (HC). (a) IL-18 was significantly elevated in lupus nephritis-IV compared to III ( p = 0.002), LN-V (0.01), and HC ( p
    Figure Legend Snippet: Comparison of serum levels Th1 cytokines (IFN- γ , IL-18, and IL-12p70), Th2 cytokine (IL-4), and Th17 cytokines (IL-17A and IL-6) in the LN classes and healthy control (HC). (a) IL-18 was significantly elevated in lupus nephritis-IV compared to III ( p = 0.002), LN-V (0.01), and HC ( p

    Techniques Used:

    Localized expressions of IL-18, IL-17A, and IFN- γ in renal tissues of patients with LN-III, LN-IV, and LN-V. Expression of IL-18, IFN- γ , and IL-17A as shown in the level of brown color (dark to light) was detected in LN-IV patients ( n = 5), LN-III patients ( n = 3), and LN-V patients ( n = 2), respectively. The cytokines expressions in infiltrating mononuclear cells (MNCs) of glomerulus were examined from 0 to 3 and glomeruli were analyzed (original magnification 200x). ∗∗ p value
    Figure Legend Snippet: Localized expressions of IL-18, IL-17A, and IFN- γ in renal tissues of patients with LN-III, LN-IV, and LN-V. Expression of IL-18, IFN- γ , and IL-17A as shown in the level of brown color (dark to light) was detected in LN-IV patients ( n = 5), LN-III patients ( n = 3), and LN-V patients ( n = 2), respectively. The cytokines expressions in infiltrating mononuclear cells (MNCs) of glomerulus were examined from 0 to 3 and glomeruli were analyzed (original magnification 200x). ∗∗ p value

    Techniques Used: Expressing

    13) Product Images from "Differential control of CD4+ T cell subsets by the PD-1/PD-L1 axis in allergic asthma"

    Article Title: Differential control of CD4+ T cell subsets by the PD-1/PD-L1 axis in allergic asthma

    Journal: European journal of immunology

    doi: 10.1002/eji.201444778

    Experimental asthma was induced, and PD-1 or PD-L1 was blocked as described in Materials and Methods. At sacrifice, AHR (A) and BAL cellularity (B) were assessed. IL-17A (C), IL-4 (D), IL-5 (E) and IL-13 (F) production by lung cells re-stimulated with
    Figure Legend Snippet: Experimental asthma was induced, and PD-1 or PD-L1 was blocked as described in Materials and Methods. At sacrifice, AHR (A) and BAL cellularity (B) were assessed. IL-17A (C), IL-4 (D), IL-5 (E) and IL-13 (F) production by lung cells re-stimulated with

    Techniques Used:

    14) Product Images from "IL-1β-MyD88-mTOR Axis Promotes Immune-Protective IL-17A+Foxp3+ Cells During Mucosal Infection and Is Dysregulated With Aging"

    Article Title: IL-1β-MyD88-mTOR Axis Promotes Immune-Protective IL-17A+Foxp3+ Cells During Mucosal Infection and Is Dysregulated With Aging

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.595936

    Loss of MyD88 in T regs diminishes IL-17A and increases IFN- γ expression in effector cells and Foxp3 + cells in vivo during oropharyngeal candidiasis (OPC) infection. FYcre and MFYcre mice were infected with sham control or CA as in Figure 2 (n= 4–6/group). On day 3 after infection, cells from cervical lymph nodes (CLN) and mouse oral intra-epithelial and lamina propria leukocytes (MOIL) were restimulated with PMA-ionomycin to assess intracellular proteins. MOIL cells pooled from 2 mice were used for flow cytometric analyses. (A) Flow plots of IL-17A and IFN-γ, gated on CD3 + CD4 + Foxp3 - effector (T eff ) cells. Statistical analyses of T eff IL-17A + (B) , and T eff IFN-γ + (C) . Flow plots of ROR-γt and IL-17A (D) , Foxp3 and IFN-γ, (F) gated on CD3 + CD4 + Foxp3 + T reg cells, and statistical analyses of the proportion of ROR-γt + IL-17A + T regs (% T reg17 ) (E) , and T reg IFN-γ + (% T regDys ) (G) , in different groups are shown. For statistical analyses, data are pooled from two experiments. Each data point represents one mouse in CLN and 2 mice in MOIL. Mean values ± SEM are plotted. (H) Correlation curve was plotted using values from tongue inflammation score, %MOIL T reg 17 and %MOIL T regDys in infected mouse groups. These data represent three independent experiments showing similar results. *P
    Figure Legend Snippet: Loss of MyD88 in T regs diminishes IL-17A and increases IFN- γ expression in effector cells and Foxp3 + cells in vivo during oropharyngeal candidiasis (OPC) infection. FYcre and MFYcre mice were infected with sham control or CA as in Figure 2 (n= 4–6/group). On day 3 after infection, cells from cervical lymph nodes (CLN) and mouse oral intra-epithelial and lamina propria leukocytes (MOIL) were restimulated with PMA-ionomycin to assess intracellular proteins. MOIL cells pooled from 2 mice were used for flow cytometric analyses. (A) Flow plots of IL-17A and IFN-γ, gated on CD3 + CD4 + Foxp3 - effector (T eff ) cells. Statistical analyses of T eff IL-17A + (B) , and T eff IFN-γ + (C) . Flow plots of ROR-γt and IL-17A (D) , Foxp3 and IFN-γ, (F) gated on CD3 + CD4 + Foxp3 + T reg cells, and statistical analyses of the proportion of ROR-γt + IL-17A + T regs (% T reg17 ) (E) , and T reg IFN-γ + (% T regDys ) (G) , in different groups are shown. For statistical analyses, data are pooled from two experiments. Each data point represents one mouse in CLN and 2 mice in MOIL. Mean values ± SEM are plotted. (H) Correlation curve was plotted using values from tongue inflammation score, %MOIL T reg 17 and %MOIL T regDys in infected mouse groups. These data represent three independent experiments showing similar results. *P

    Techniques Used: Expressing, In Vivo, Infection, Mouse Assay

    T reg 17 and T regDys alterations are accompanied by loss of IRAK-4 and mTOR activation in T regs in infected aged mice. Young and Aged mice were infected with CA or Sham as in Figure 8 . Flow cytometric analyses of CD3 + CD4 + gated cells for IL-17A and Foxp3 (A, B) , IFN-γ and Foxp3 (C, D) , on day 5 after infection. Statistical analyses of Foxp3 + IL-17A + (% T reg 17) (B) , and Foxp3 + IFN-γ + (% T regDys ) (D) , using data points from (A, B) , respectively. (E, F) Mice were infected for 2 days. Flow cytometry analyses of CD3 + CD4 + Foxp3 + gated cells for p-IRAK and IL-1R ( E , left and right) and p-mTOR ( F , top and bottom) in Foxp3 + T regs . Geometric mean fluorescence intensities (GM) are indicated in the histogram plots. Mean values ± SEM are plotted in statistical analyses. These data show one of four independent experiments showing similar results. (*P
    Figure Legend Snippet: T reg 17 and T regDys alterations are accompanied by loss of IRAK-4 and mTOR activation in T regs in infected aged mice. Young and Aged mice were infected with CA or Sham as in Figure 8 . Flow cytometric analyses of CD3 + CD4 + gated cells for IL-17A and Foxp3 (A, B) , IFN-γ and Foxp3 (C, D) , on day 5 after infection. Statistical analyses of Foxp3 + IL-17A + (% T reg 17) (B) , and Foxp3 + IFN-γ + (% T regDys ) (D) , using data points from (A, B) , respectively. (E, F) Mice were infected for 2 days. Flow cytometry analyses of CD3 + CD4 + Foxp3 + gated cells for p-IRAK and IL-1R ( E , left and right) and p-mTOR ( F , top and bottom) in Foxp3 + T regs . Geometric mean fluorescence intensities (GM) are indicated in the histogram plots. Mean values ± SEM are plotted in statistical analyses. These data show one of four independent experiments showing similar results. (*P

    Techniques Used: Activation Assay, Infection, Mouse Assay, Flow Cytometry, Fluorescence

    IL-1β promotes T reg 17 cells and constrains induction of T regDys cells in Candida activated oral mucosal cells in vitro . (A) HKGT mediated T reg 17 induction is slightly reduced in TLR-2 -/- T regs , but significantly lower in MFYcre T regs in vitro . Pooled cervical lymph nodes (CLN) and mouse oral intra-epithelial and lamina propria leukocytes (MOIL) cells from FYcre and MFYcre were stimulated with heat killed Candida albicans germ tube (HKGT) as in Figure 1C for 5 days and were restimulated with PMA-Ionomycin for flow cytometry assessment. For TLR-2 -/- cultures, T regs were purified from TLR-2 -/- mice and stimulated with WT APC from T cell depleted CLN and MOIL cells. Flow cytometry plots showing Foxp3 and IL-17A expression (gated on CD3 + CD4 + Foxp3 + cells). (B, C) WT CLN and MOIL cells were examined ex vivo or stimulated with HKGT as in Figure 1C , in the presence of IL-1β (10 ng/ml) or α- IL-β antibody (10 μg/ml) for 5 days. Flow cytometry plots showing Foxp3 and IL-17A expression (gated on CD3 + CD4 + cells) ( B , top) and statistical data points from experimental replicates ( B , bottom). Flow plots showing Foxp3 and IFN-γ expression (gated on CD3 + CD4 + Foxp3 + cells) (C) and statistical data points from experimental replicates ( C , bottom). NS, non-significant. *P
    Figure Legend Snippet: IL-1β promotes T reg 17 cells and constrains induction of T regDys cells in Candida activated oral mucosal cells in vitro . (A) HKGT mediated T reg 17 induction is slightly reduced in TLR-2 -/- T regs , but significantly lower in MFYcre T regs in vitro . Pooled cervical lymph nodes (CLN) and mouse oral intra-epithelial and lamina propria leukocytes (MOIL) cells from FYcre and MFYcre were stimulated with heat killed Candida albicans germ tube (HKGT) as in Figure 1C for 5 days and were restimulated with PMA-Ionomycin for flow cytometry assessment. For TLR-2 -/- cultures, T regs were purified from TLR-2 -/- mice and stimulated with WT APC from T cell depleted CLN and MOIL cells. Flow cytometry plots showing Foxp3 and IL-17A expression (gated on CD3 + CD4 + Foxp3 + cells). (B, C) WT CLN and MOIL cells were examined ex vivo or stimulated with HKGT as in Figure 1C , in the presence of IL-1β (10 ng/ml) or α- IL-β antibody (10 μg/ml) for 5 days. Flow cytometry plots showing Foxp3 and IL-17A expression (gated on CD3 + CD4 + cells) ( B , top) and statistical data points from experimental replicates ( B , bottom). Flow plots showing Foxp3 and IFN-γ expression (gated on CD3 + CD4 + Foxp3 + cells) (C) and statistical data points from experimental replicates ( C , bottom). NS, non-significant. *P

    Techniques Used: In Vitro, Flow Cytometry, Purification, Mouse Assay, Expressing, Ex Vivo

    Loss of endogenous IL-1R signaling diminishes T reg 17 but increases T regDys cells in CD4 + T cells in vitro . IL-6 expands T regDys cells in the absence of IL-1β. Naïve CD4 + T cells from WT C57BL/6 and IL-1R (IL-1R1 -/- ) knockout mice were stimulated with heat killed Candida albicans germ tube (HKGT) and TGF-β1 for 5 days as in Figure 1C with WT APC and stained for Foxp3, IL-17A ( A , top and bottom), and Foxp3 and IFN-γ ( B , top and bottom) (gated on CD3 + CD4 + cells). (C) Naïve CD4 + T cells from WT C57BL/6 and IL-1R (IL-1R1 -/- ) knockout mice were stimulated with HKGT and TGF-β1 for 3 days. CD4 T cells were purified from these cultures for qPCR assessment of indicated transcripts. (D, E) WT and IL-1R1 -/- mice were orally infected with CA, and cervical lymph nodes (CLN) and mouse oral intra-epithelial and lamina propria leukocytes (MOIL) were collected 3 days after infection (n=5/group) for HKGT restimulation for 2 days and flow cytometry. Statistical analyses of Foxp3 + ROR-γt + IL-17A + (D) , and Foxp3 + IFN-γ + (E) , expressing cells after PMA/Iono restimulation for 4 h in vitro (gated on CD3 + CD4 + cells). α- IL-6 antibody (10 μg/ml) was added in some cultures. Data are representative of at least 3 independent experiments. NS, non-significant. *P
    Figure Legend Snippet: Loss of endogenous IL-1R signaling diminishes T reg 17 but increases T regDys cells in CD4 + T cells in vitro . IL-6 expands T regDys cells in the absence of IL-1β. Naïve CD4 + T cells from WT C57BL/6 and IL-1R (IL-1R1 -/- ) knockout mice were stimulated with heat killed Candida albicans germ tube (HKGT) and TGF-β1 for 5 days as in Figure 1C with WT APC and stained for Foxp3, IL-17A ( A , top and bottom), and Foxp3 and IFN-γ ( B , top and bottom) (gated on CD3 + CD4 + cells). (C) Naïve CD4 + T cells from WT C57BL/6 and IL-1R (IL-1R1 -/- ) knockout mice were stimulated with HKGT and TGF-β1 for 3 days. CD4 T cells were purified from these cultures for qPCR assessment of indicated transcripts. (D, E) WT and IL-1R1 -/- mice were orally infected with CA, and cervical lymph nodes (CLN) and mouse oral intra-epithelial and lamina propria leukocytes (MOIL) were collected 3 days after infection (n=5/group) for HKGT restimulation for 2 days and flow cytometry. Statistical analyses of Foxp3 + ROR-γt + IL-17A + (D) , and Foxp3 + IFN-γ + (E) , expressing cells after PMA/Iono restimulation for 4 h in vitro (gated on CD3 + CD4 + cells). α- IL-6 antibody (10 μg/ml) was added in some cultures. Data are representative of at least 3 independent experiments. NS, non-significant. *P

    Techniques Used: In Vitro, Knock-Out, Mouse Assay, Staining, Purification, Real-time Polymerase Chain Reaction, Infection, Flow Cytometry, Expressing

    15) Product Images from "Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease"

    Article Title: Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease

    Journal: Clinical and Experimental Immunology

    doi: 10.1111/cei.12084

    Infliximab (IFX) suppresses Crohn's disease (CD) lamina propria (LP) CD4 + T cell interleukin (IL)-21 and IL-17A mRNA expression and T helper type 17 (Th17) cell differentiation. Purified LP-CD4 + T cells (1 × 10 6 /ml) from CD patients and healthy
    Figure Legend Snippet: Infliximab (IFX) suppresses Crohn's disease (CD) lamina propria (LP) CD4 + T cell interleukin (IL)-21 and IL-17A mRNA expression and T helper type 17 (Th17) cell differentiation. Purified LP-CD4 + T cells (1 × 10 6 /ml) from CD patients and healthy

    Techniques Used: Expressing, Cell Differentiation, Purification

    Infliximab (IFX) decreases interleukin (IL)-21- and IL-17A-positive cell infiltration in the inflamed mucosa of Crohn's disease (CD) patients. Representative frozen sections were obtained from inflamed mucosa of CD patients before (a,c) and at week 10
    Figure Legend Snippet: Infliximab (IFX) decreases interleukin (IL)-21- and IL-17A-positive cell infiltration in the inflamed mucosa of Crohn's disease (CD) patients. Representative frozen sections were obtained from inflamed mucosa of CD patients before (a,c) and at week 10

    Techniques Used:

    16) Product Images from "IL-17A Mediates a Selective Gene Expression Profile in Asthmatic Human Airway Smooth Muscle Cells"

    Article Title: IL-17A Mediates a Selective Gene Expression Profile in Asthmatic Human Airway Smooth Muscle Cells

    Journal: American Journal of Respiratory Cell and Molecular Biology

    doi: 10.1165/rcmb.2012-0267OC

    Validation of IL-17A gene targets by quantitative RT-PCR (qRT-PCR). Response levels from nine genes selected from the GeneChip array analysis were confirmed in independent experiments by qRT-PCR. Synergistic responses between IL-17A and IL-1β or IL-22 (10 ng/ml) were equally assessed in primary human ASM cells from three individuals with atopy and asthma and three healthy, normal donors. Results were normalized to respective glyceraldehyde 3-phosphate dehydrogenase levels, and are presented as the mean fold increase over unstimulated controls at 2 and 6 hours after cytokine stimulation.
    Figure Legend Snippet: Validation of IL-17A gene targets by quantitative RT-PCR (qRT-PCR). Response levels from nine genes selected from the GeneChip array analysis were confirmed in independent experiments by qRT-PCR. Synergistic responses between IL-17A and IL-1β or IL-22 (10 ng/ml) were equally assessed in primary human ASM cells from three individuals with atopy and asthma and three healthy, normal donors. Results were normalized to respective glyceraldehyde 3-phosphate dehydrogenase levels, and are presented as the mean fold increase over unstimulated controls at 2 and 6 hours after cytokine stimulation.

    Techniques Used: Quantitative RT-PCR

    Activation state and synergistic response of the regulatory NF-κB network. Immortalized human telomerase reverse transcriptase (hTERT) ASM cells from three independent individuals were stimulated with IL-17A or IL-1β (10 ng/ml) ( A ) for up to 1 hour, or ( B ) in combination for 15 minutes. Cytoplasmic and nuclear fractions were extracted and protein levels of NF-κB regulatory factors were assessed. C, cytoplasmic; IKK, IκB kinase; N, nuclear.
    Figure Legend Snippet: Activation state and synergistic response of the regulatory NF-κB network. Immortalized human telomerase reverse transcriptase (hTERT) ASM cells from three independent individuals were stimulated with IL-17A or IL-1β (10 ng/ml) ( A ) for up to 1 hour, or ( B ) in combination for 15 minutes. Cytoplasmic and nuclear fractions were extracted and protein levels of NF-κB regulatory factors were assessed. C, cytoplasmic; IKK, IκB kinase; N, nuclear.

    Techniques Used: Activation Assay

    Nuclear translocation and synergistic response of MAPKs. Immortalized hTERT ASM cells from three independent individuals were stimulated with IL-17A or IL-1β (10 ng/ml) ( A ) for up to 1 hour, or ( B ) in combination for 15 minutes. Cytoplasmic and nuclear fractions were extracted and MAPK levels were assessed.
    Figure Legend Snippet: Nuclear translocation and synergistic response of MAPKs. Immortalized hTERT ASM cells from three independent individuals were stimulated with IL-17A or IL-1β (10 ng/ml) ( A ) for up to 1 hour, or ( B ) in combination for 15 minutes. Cytoplasmic and nuclear fractions were extracted and MAPK levels were assessed.

    Techniques Used: Translocation Assay

    Activation of the mitogen-activated protein kinase (MAPK) signal transduction pathway. Immortalized hTERT ASM cells from three independent individuals were stimulated with IL-17A or IL-1β (10 ng/ml) for up to 1 hour. ( A ) Profiling antibody array and validation by ( B ) Western blot was used to quantify MAPK kinases at 5, 15, 30, and 60 minutes after stimulation. ERK, extracellular signal–regulated kinase; JNK, c-Jun NH2-terminal kinase; MSK, mitogen and stress–activated protein kinase; RSK, ribosomal S6 kinase.
    Figure Legend Snippet: Activation of the mitogen-activated protein kinase (MAPK) signal transduction pathway. Immortalized hTERT ASM cells from three independent individuals were stimulated with IL-17A or IL-1β (10 ng/ml) for up to 1 hour. ( A ) Profiling antibody array and validation by ( B ) Western blot was used to quantify MAPK kinases at 5, 15, 30, and 60 minutes after stimulation. ERK, extracellular signal–regulated kinase; JNK, c-Jun NH2-terminal kinase; MSK, mitogen and stress–activated protein kinase; RSK, ribosomal S6 kinase.

    Techniques Used: Activation Assay, Transduction, Ab Array, Western Blot

    Related Articles

    Concentration Assay:

    Article Title: FTY720 controls disease severity and attenuates sciatic nerve damage in chronic experimental autoimmune neuritis
    Article Snippet: .. The concentration of IL-17 cytokine was measured in duplicate in undiluted sera using commercial ELISA kits specific for rat IL-17 (eBioscience, San Diego, CA, USA), as per the manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: FTY720 controls disease severity and attenuates sciatic nerve damage in chronic experimental autoimmune neuritis
    Article Snippet: .. The concentration of IL-17 cytokine was measured in duplicate in undiluted sera using commercial ELISA kits specific for rat IL-17 (eBioscience, San Diego, CA, USA), as per the manufacturer’s instructions. ..

    Article Title: Intranasal Administration of Recombinant Mycobacterium smegmatis Inducing IL-17A Autoantibody Attenuates Airway Inflammation in a Murine Model of Allergic Asthma
    Article Snippet: Cytokine levels in BALF and culture supernatant of splenocytes ELISA was performed according to the manufacturer’s instructions. .. The concentrations of cytokine in BALF and culture supernatant of splenocytes and mediastinal lymph nodes were measured using specific mouse IL-5, IL-6, IL-10, IL-12, IL-13 and IL-17A ELISA kits (eBioscience). .. Quantitative RT-PCR analysis The mRNA expression levels were assessed by quantitative real-time polymerase chain reaction (RT-PCR).

    Article Title: Expression of Interleukin-17A in Lung Tissues of Irradiated Mice and the Influence of Dexamethasone
    Article Snippet: Animals and Reagents Inbred male C57BL/6J mice (21 ± 2 g, 6–8 weeks) were obtained from Vital River Laboratory Animal Technology. .. The ELISA assay kits for mouse IL-17A, TGF-β 1, TNF-α , and IL-6 were purchased from eBioscience (San Diego, CA, USA). .. The mouse IL-17A polyclonal Ab for immunohistochemical analysis was obtained from Abcam (Abcam Inc., Cambridge, MA, USA).

    Article Title: Sishen Pill Treatment of DSS-Induced Colitis via Regulating Interaction With Inflammatory Dendritic Cells and Gut Microbiota
    Article Snippet: Total DNA extraction kit, total RNA extraction kit, First-Stand cDNA reverse transcription kit, polymerase chain reaction kit, and primers were obtained from Shanghai Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). .. Mouse IL-4, IL-9, IL-17A, and IL-1β ELISA kits were obtained from Thermo Scientific Co. Ltd. (Thermo Fisher, Massachusetts, USA). .. PE anti-CD11c, AF647 anti-CD103, APC anti-TNF-α, and FITC anti-E-cadherin for flow cytometry were purchased from BD Biosciences (Franklin Lakes, NJ, USA).

    Article Title: Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment
    Article Snippet: PBMC were thawed and incubated at 1 × 106 per ml in the presence of carnosol (5 µM), curcumin (5 µM), or a vehicle control (DMSO) for 6 hours prior to stimulation with anti-CD3 (1 µg/ml; eBioscience). .. After 4 days supernatants were removed for analysis of IFNγ and IL-17A concentrations by ELISA (Ready-Set-Go kit; eBioscience), and PBMC were restimulated for flow cytometry. .. Dendritic cell culture CD14+ monocytes were positively selected from PBMC by magnetic sorting using a MagniSort Human CD14 Positive Selection kit (eBioscience) according to the manufacturer’s protocol.

    Article Title: Concomitant suppression of TH2 and TH17 cell responses in allergic asthma by targeting retinoic acid receptor–related orphan receptor γt
    Article Snippet: For mouse IL-17A, ELISA kits were from BioLegend. .. For mouse IL-13 and human IL-13 and IL-17A, ELISA kits were from eBioscience. ..

    Article Title: Pharmacological inhibition of RORγt suppresses the Th17 pathway and alleviates arthritis in vivo
    Article Snippet: .. Supernatants were removed and IL-17A cytokine concentrations were determined by ELISA according to the manufacturer`s specifications (eBioscience). .. The numbers of IL-17A producing, antigen-specific cells were quantified by ELISPOT as recently described [ ].

    Article Title: Role of the Gut-associated and Secondary Lymphoid Tissue in the Induction of Chronic Colitis
    Article Snippet: To determine extracellular cytokine production, colonic LPMCs (1.0 × 105 cells) were cultured for 16 hours in 200 µL of complete RPMI-1640 medium at 37°C and 5% CO2 in an anti-CD3ε mAb-coated 96-well flat-bottom plate with 1 µg/mL soluble anti-CD28 mAb. .. IFN-γ and IL-17A levels in the supernatants were determined by sandwich enzyme-linked immunosorbent assay (ELISA) using the commercial kits Mouse IFN-γ or IL-17A ELISA (eBioscience) as described by the manufacturer. ..

    Flow Cytometry:

    Article Title: Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment
    Article Snippet: PBMC were thawed and incubated at 1 × 106 per ml in the presence of carnosol (5 µM), curcumin (5 µM), or a vehicle control (DMSO) for 6 hours prior to stimulation with anti-CD3 (1 µg/ml; eBioscience). .. After 4 days supernatants were removed for analysis of IFNγ and IL-17A concentrations by ELISA (Ready-Set-Go kit; eBioscience), and PBMC were restimulated for flow cytometry. .. Dendritic cell culture CD14+ monocytes were positively selected from PBMC by magnetic sorting using a MagniSort Human CD14 Positive Selection kit (eBioscience) according to the manufacturer’s protocol.

    Cytometry:

    Article Title: Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment
    Article Snippet: PBMC were thawed and incubated at 1 × 106 per ml in the presence of carnosol (5 µM), curcumin (5 µM), or a vehicle control (DMSO) for 6 hours prior to stimulation with anti-CD3 (1 µg/ml; eBioscience). .. After 4 days supernatants were removed for analysis of IFNγ and IL-17A concentrations by ELISA (Ready-Set-Go kit; eBioscience), and PBMC were restimulated for flow cytometry. .. Dendritic cell culture CD14+ monocytes were positively selected from PBMC by magnetic sorting using a MagniSort Human CD14 Positive Selection kit (eBioscience) according to the manufacturer’s protocol.

    Sandwich ELISA:

    Article Title: Role of the Gut-associated and Secondary Lymphoid Tissue in the Induction of Chronic Colitis
    Article Snippet: To determine extracellular cytokine production, colonic LPMCs (1.0 × 105 cells) were cultured for 16 hours in 200 µL of complete RPMI-1640 medium at 37°C and 5% CO2 in an anti-CD3ε mAb-coated 96-well flat-bottom plate with 1 µg/mL soluble anti-CD28 mAb. .. IFN-γ and IL-17A levels in the supernatants were determined by sandwich enzyme-linked immunosorbent assay (ELISA) using the commercial kits Mouse IFN-γ or IL-17A ELISA (eBioscience) as described by the manufacturer. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher tnf α
    Indoleamine 2,3 dioxygenase-1 (IDO1) influences the gene expression of cytokines and transcription factors. Relative expression of mRNA for aryl hydrocarbon receptor (AhR), IFN-γ, <t>TNF-α,</t> IL-6, RORC, Tbet, GATA3, FoxP3, IL-10, TGF-β, IL-17, and IL-22 in whole lung cells of wild-type and IDO1 −/− mice after 10 weeks of Paracoccidioides brasiliensis infection. The level of gene transcription was determined by real-time PCR. Bars show mean ± SEM from at least four mice per group and are representative of three independent experiments (* p
    Tnf α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher mouse il 17a
    IHC analysis of the effect of BSYSC on <t>IL-17A</t> and FoxP3 protein expressions in the brain and spinal cord of mice. Note: ** p
    Mouse Il 17a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 17a/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse il 17a - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher anti human il 17
    Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular <t>IL-17</t> (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p
    Anti Human Il 17, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human il 17/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human il 17 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    97
    Thermo Fisher anti il17 antibody
    IL-17 production during Salmonella infection in WT and Crtam −/− mice. Immune cells from the gut of infected mice were isolated, then stimulated with PMA and ionomycin in the presence of brefeldin-A. After 6 hours of stimulation, cells were stained for intracellular IL-17, then analyzed by flow cytometry. ( a ) Representative contour plots of CD3 + CD4 + IL-17 + and CD3 + CD4 + CD8 + IL-17 + cells obtained from the gut of S . Typhimurium-infected WT ( n =12) or Crtam −/− ( n =11) mice are shown. ( b ) Frequency of IL-17-producing cells per million live cells was calculated for each T cell subset. Data shown comprise three independent experiments. ( c, d ) Cells from the small intestine were fractionated into ( c ) intraepithelial lymphocytes (IEL) and ( d ) lamina propria lymphocytes (LPL), then treated as mentioned above. Frequency of IL-17-producing cells per million live cells was calculated for each indicated T cell subset. ( b, c, d ) Each black circle (WT) or white square ( Crtam −/− ) represents a mouse, and bars represent the average of each group. ( e ) Relative expression levels (qPCR) of Il1b, Il6, Il23, and Tgfb in the cecum of WT (black bars, n =8) or Crtam −/− (white bars, n =11) mice at 96h post- S . Typhimurium infection, compared to uninfected controls. ( f ) Relative expression levels (qPCR) of <t>Il17,</t> Il22, Lcn2, and Cxcl1 in the cecum of WT (black bars, n =10) or Crtam −/− (white bars, n =8) mice at 96h post- S . Typhimurium infection, compared to uninfected controls. Expression of Actb was used as a housekeeping control. Data represent the mean ± standard error. Data shown comprise three independent experiments. n.s. = not significant. A significant difference (t-test) is indicated by * ( P ≤ 0.05) or ** ( P ≤ 0.01).
    Anti Il17 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti il17 antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti il17 antibody - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    Indoleamine 2,3 dioxygenase-1 (IDO1) influences the gene expression of cytokines and transcription factors. Relative expression of mRNA for aryl hydrocarbon receptor (AhR), IFN-γ, TNF-α, IL-6, RORC, Tbet, GATA3, FoxP3, IL-10, TGF-β, IL-17, and IL-22 in whole lung cells of wild-type and IDO1 −/− mice after 10 weeks of Paracoccidioides brasiliensis infection. The level of gene transcription was determined by real-time PCR. Bars show mean ± SEM from at least four mice per group and are representative of three independent experiments (* p

    Journal: Frontiers in Immunology

    Article Title: The IDO–AhR Axis Controls Th17/Treg Immunity in a Pulmonary Model of Fungal Infection

    doi: 10.3389/fimmu.2017.00880

    Figure Lengend Snippet: Indoleamine 2,3 dioxygenase-1 (IDO1) influences the gene expression of cytokines and transcription factors. Relative expression of mRNA for aryl hydrocarbon receptor (AhR), IFN-γ, TNF-α, IL-6, RORC, Tbet, GATA3, FoxP3, IL-10, TGF-β, IL-17, and IL-22 in whole lung cells of wild-type and IDO1 −/− mice after 10 weeks of Paracoccidioides brasiliensis infection. The level of gene transcription was determined by real-time PCR. Bars show mean ± SEM from at least four mice per group and are representative of three independent experiments (* p

    Article Snippet: The levels of IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-17, IL-22, IL-27, IL-35, IL-23, TNF-α, IFN-γ, and TGF-β were measured by capture enzyme-linked immunosorbent assay (ELISA) with antibody pairs purchased from eBioscience or PBL.

    Techniques: Expressing, Mouse Assay, Infection, Real-time Polymerase Chain Reaction

    Indoleamine 2,3 dioxygenase-1 (IDO1) expression controls the presence of intracellular cytokines in pulmonary CD11b + and CD11c + cells. The intracellular cytokines were determined in CD11b + (A) and CD11c + (B) lung infiltrating leukocytes of wild-type and IDO1 −/− C57BL/6 mice after 96 h, 2, and 10 weeks of infection with 1 × 10 6 Paracoccidioides brasiliensis yeasts. The lung cells were obtained as described in Section “ Materials and Methods ” and labeled with antibodies conjugated to different fluorochromes. The lung infiltrating leukocytes were gated by FSC/SSC analysis. The cells were gated for CD11b + or CD11c + expression and then for the presence of cytokines IL-12, TNF-α, IL-1β, IL-10, IL-6, and TGF-β. One hundred thousand cells were acquired on FACS CANTO II and subsequently analyzed by FlowJo software. Data are expressed as means ± SEM and are representative of three independent experiments using five mice of each mouse strain per group (* p

    Journal: Frontiers in Immunology

    Article Title: The IDO–AhR Axis Controls Th17/Treg Immunity in a Pulmonary Model of Fungal Infection

    doi: 10.3389/fimmu.2017.00880

    Figure Lengend Snippet: Indoleamine 2,3 dioxygenase-1 (IDO1) expression controls the presence of intracellular cytokines in pulmonary CD11b + and CD11c + cells. The intracellular cytokines were determined in CD11b + (A) and CD11c + (B) lung infiltrating leukocytes of wild-type and IDO1 −/− C57BL/6 mice after 96 h, 2, and 10 weeks of infection with 1 × 10 6 Paracoccidioides brasiliensis yeasts. The lung cells were obtained as described in Section “ Materials and Methods ” and labeled with antibodies conjugated to different fluorochromes. The lung infiltrating leukocytes were gated by FSC/SSC analysis. The cells were gated for CD11b + or CD11c + expression and then for the presence of cytokines IL-12, TNF-α, IL-1β, IL-10, IL-6, and TGF-β. One hundred thousand cells were acquired on FACS CANTO II and subsequently analyzed by FlowJo software. Data are expressed as means ± SEM and are representative of three independent experiments using five mice of each mouse strain per group (* p

    Article Snippet: The levels of IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-17, IL-22, IL-27, IL-35, IL-23, TNF-α, IFN-γ, and TGF-β were measured by capture enzyme-linked immunosorbent assay (ELISA) with antibody pairs purchased from eBioscience or PBL.

    Techniques: Expressing, Mouse Assay, Infection, Labeling, FACS, Software

    IHC analysis of the effect of BSYSC on IL-17A and FoxP3 protein expressions in the brain and spinal cord of mice. Note: ** p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis

    doi: 10.1186/s12906-015-0572-0

    Figure Lengend Snippet: IHC analysis of the effect of BSYSC on IL-17A and FoxP3 protein expressions in the brain and spinal cord of mice. Note: ** p

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-17A was purchased from eBioScience (San Diego, CA, USA), mouse IL-23 was from Beijing 4A Biotech Co., Ltd. (Beijing, China), mouse IL-6 and mouse TGF-β1 were provided by NeoBioscience Technology Co. (Beijing, China).

    Techniques: Immunohistochemistry, Mouse Assay

    qRT-PCR analysis of the effect of BSYSC on mRNA expressions of IL-17A and FoxP3 in the brain and spinal cord of mice. Note: * p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis

    doi: 10.1186/s12906-015-0572-0

    Figure Lengend Snippet: qRT-PCR analysis of the effect of BSYSC on mRNA expressions of IL-17A and FoxP3 in the brain and spinal cord of mice. Note: * p

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-17A was purchased from eBioScience (San Diego, CA, USA), mouse IL-23 was from Beijing 4A Biotech Co., Ltd. (Beijing, China), mouse IL-6 and mouse TGF-β1 were provided by NeoBioscience Technology Co. (Beijing, China).

    Techniques: Quantitative RT-PCR, Mouse Assay

    Western blot analysis of the effect of BSYSC on IL-17A and FoxP3 protein expressions in the brain and spinal cord of mice. (A) to (D) NC, EAE, EAE + PA and EAE + BSYSC mice. Note: * p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis

    doi: 10.1186/s12906-015-0572-0

    Figure Lengend Snippet: Western blot analysis of the effect of BSYSC on IL-17A and FoxP3 protein expressions in the brain and spinal cord of mice. (A) to (D) NC, EAE, EAE + PA and EAE + BSYSC mice. Note: * p

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-17A was purchased from eBioScience (San Diego, CA, USA), mouse IL-23 was from Beijing 4A Biotech Co., Ltd. (Beijing, China), mouse IL-6 and mouse TGF-β1 were provided by NeoBioscience Technology Co. (Beijing, China).

    Techniques: Western Blot, Mouse Assay

    ELISA analysis of IL-17A, IL-6, IL-23 and TGF-β1 expressions in the brain of the groups. Note: * p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis

    doi: 10.1186/s12906-015-0572-0

    Figure Lengend Snippet: ELISA analysis of IL-17A, IL-6, IL-23 and TGF-β1 expressions in the brain of the groups. Note: * p

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-17A was purchased from eBioScience (San Diego, CA, USA), mouse IL-23 was from Beijing 4A Biotech Co., Ltd. (Beijing, China), mouse IL-6 and mouse TGF-β1 were provided by NeoBioscience Technology Co. (Beijing, China).

    Techniques: Enzyme-linked Immunosorbent Assay

    Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p

    Journal: Scientific Reports

    Article Title: Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus

    doi: 10.1038/s41598-018-35161-5

    Figure Lengend Snippet: Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p

    Article Snippet: EliSpot assays ELISPOT plates (Millipore 96-well MultiScreen HA; Millipore) were coated with 2 mg/ml anti-human IL-17–specific mAb (eBio64CAP17; eBioscience) in PBS.

    Techniques: Cell Culture, Expressing, Flow Cytometry, Cytometry

    Fungi specific T cells produce IL-17 in an age dependent manner. CD4 + CD45RA + CD31 + T cells from neonates, infants, children, and adults were co-cultured with monocytes pulsed with C . albicans- or A . fumigatus- lysates. ( A , B ) The frequency of T cells expressing signature Th17 molecules IL-17 ( A ) and RORγt ( B ) were analysed by flow cytometry at day 3 (upper Panel) and day 6 after stimulation (lower panel) ( C ) Bar graph representing the ELISPOT analysis of the quantitative IL-17, produced by the T cells from neonates and adults which were either stimulated or not for 3 days as described in ( A ). ( D ) Determination of IL-17A cytokine release of CD4 + CD45RA + T cells of neonates, infants and children or adults by LegendPlex which were either stimulated or not for 3 days as described in ( A ). ( E ) Frequency of T cells expressing intracellular IL-17 (white), IL-4 (black) and both IL-17/IL-4 (grey) were measured by flow cytometry after 6 days of stimulation, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. (F) Bar graph showing IL-17 expression by CD4 + CD45RA + CD31 + T cells of neonates, adults, and infants of 0.5-2 years old, stimulated for 6 days as described in ( A ) in the presence or absence of neutralizing antibodies for IL-1ß, IL-6 or both. Cumulative results are shown and each dot in ( A – D ) and ( F ) represent a different donor. The error bars in figures denote ± SD. *p

    Journal: Scientific Reports

    Article Title: Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus

    doi: 10.1038/s41598-018-35161-5

    Figure Lengend Snippet: Fungi specific T cells produce IL-17 in an age dependent manner. CD4 + CD45RA + CD31 + T cells from neonates, infants, children, and adults were co-cultured with monocytes pulsed with C . albicans- or A . fumigatus- lysates. ( A , B ) The frequency of T cells expressing signature Th17 molecules IL-17 ( A ) and RORγt ( B ) were analysed by flow cytometry at day 3 (upper Panel) and day 6 after stimulation (lower panel) ( C ) Bar graph representing the ELISPOT analysis of the quantitative IL-17, produced by the T cells from neonates and adults which were either stimulated or not for 3 days as described in ( A ). ( D ) Determination of IL-17A cytokine release of CD4 + CD45RA + T cells of neonates, infants and children or adults by LegendPlex which were either stimulated or not for 3 days as described in ( A ). ( E ) Frequency of T cells expressing intracellular IL-17 (white), IL-4 (black) and both IL-17/IL-4 (grey) were measured by flow cytometry after 6 days of stimulation, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. (F) Bar graph showing IL-17 expression by CD4 + CD45RA + CD31 + T cells of neonates, adults, and infants of 0.5-2 years old, stimulated for 6 days as described in ( A ) in the presence or absence of neutralizing antibodies for IL-1ß, IL-6 or both. Cumulative results are shown and each dot in ( A – D ) and ( F ) represent a different donor. The error bars in figures denote ± SD. *p

    Article Snippet: EliSpot assays ELISPOT plates (Millipore 96-well MultiScreen HA; Millipore) were coated with 2 mg/ml anti-human IL-17–specific mAb (eBio64CAP17; eBioscience) in PBS.

    Techniques: Cell Culture, Expressing, Flow Cytometry, Cytometry, Enzyme-linked Immunospot, Produced

    IL-17 production during Salmonella infection in WT and Crtam −/− mice. Immune cells from the gut of infected mice were isolated, then stimulated with PMA and ionomycin in the presence of brefeldin-A. After 6 hours of stimulation, cells were stained for intracellular IL-17, then analyzed by flow cytometry. ( a ) Representative contour plots of CD3 + CD4 + IL-17 + and CD3 + CD4 + CD8 + IL-17 + cells obtained from the gut of S . Typhimurium-infected WT ( n =12) or Crtam −/− ( n =11) mice are shown. ( b ) Frequency of IL-17-producing cells per million live cells was calculated for each T cell subset. Data shown comprise three independent experiments. ( c, d ) Cells from the small intestine were fractionated into ( c ) intraepithelial lymphocytes (IEL) and ( d ) lamina propria lymphocytes (LPL), then treated as mentioned above. Frequency of IL-17-producing cells per million live cells was calculated for each indicated T cell subset. ( b, c, d ) Each black circle (WT) or white square ( Crtam −/− ) represents a mouse, and bars represent the average of each group. ( e ) Relative expression levels (qPCR) of Il1b, Il6, Il23, and Tgfb in the cecum of WT (black bars, n =8) or Crtam −/− (white bars, n =11) mice at 96h post- S . Typhimurium infection, compared to uninfected controls. ( f ) Relative expression levels (qPCR) of Il17, Il22, Lcn2, and Cxcl1 in the cecum of WT (black bars, n =10) or Crtam −/− (white bars, n =8) mice at 96h post- S . Typhimurium infection, compared to uninfected controls. Expression of Actb was used as a housekeeping control. Data represent the mean ± standard error. Data shown comprise three independent experiments. n.s. = not significant. A significant difference (t-test) is indicated by * ( P ≤ 0.05) or ** ( P ≤ 0.01).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: CRTAM shapes the gut microbiota and enhances the severity of infection

    doi: 10.4049/jimmunol.1800890

    Figure Lengend Snippet: IL-17 production during Salmonella infection in WT and Crtam −/− mice. Immune cells from the gut of infected mice were isolated, then stimulated with PMA and ionomycin in the presence of brefeldin-A. After 6 hours of stimulation, cells were stained for intracellular IL-17, then analyzed by flow cytometry. ( a ) Representative contour plots of CD3 + CD4 + IL-17 + and CD3 + CD4 + CD8 + IL-17 + cells obtained from the gut of S . Typhimurium-infected WT ( n =12) or Crtam −/− ( n =11) mice are shown. ( b ) Frequency of IL-17-producing cells per million live cells was calculated for each T cell subset. Data shown comprise three independent experiments. ( c, d ) Cells from the small intestine were fractionated into ( c ) intraepithelial lymphocytes (IEL) and ( d ) lamina propria lymphocytes (LPL), then treated as mentioned above. Frequency of IL-17-producing cells per million live cells was calculated for each indicated T cell subset. ( b, c, d ) Each black circle (WT) or white square ( Crtam −/− ) represents a mouse, and bars represent the average of each group. ( e ) Relative expression levels (qPCR) of Il1b, Il6, Il23, and Tgfb in the cecum of WT (black bars, n =8) or Crtam −/− (white bars, n =11) mice at 96h post- S . Typhimurium infection, compared to uninfected controls. ( f ) Relative expression levels (qPCR) of Il17, Il22, Lcn2, and Cxcl1 in the cecum of WT (black bars, n =10) or Crtam −/− (white bars, n =8) mice at 96h post- S . Typhimurium infection, compared to uninfected controls. Expression of Actb was used as a housekeeping control. Data represent the mean ± standard error. Data shown comprise three independent experiments. n.s. = not significant. A significant difference (t-test) is indicated by * ( P ≤ 0.05) or ** ( P ≤ 0.01).

    Article Snippet: After surface staining, cells were fixed and permeabilized according to the manufacturer’s instructions (“Fix and Perm” kit, eBioscience), then stained intracellularly with anti-IL17 antibody (clone TC11–18H10.1), anti-IFNγ antibody (clone XMG1.2), and anti-IL-10 antibody (clone JES5–16-E3) from eBioscience.

    Techniques: Infection, Mouse Assay, Isolation, Staining, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, T-Test