il 17a  (Kingfisher Biotech)


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    Name:
    Dolphin IL 17A Do It Yourself ELISA
    Description:

    Catalog Number:
    DIY1101Y-003
    Price:
    700.0
    Quantity:
    1 Set
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    Structured Review

    Kingfisher Biotech il 17a
    Effect of dexamethasone on LPS-, Pam3CSK4- and <t>IL-17A-induced</t> up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

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    Average 93 stars, based on 1 article reviews
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    il 17a - by Bioz Stars, 2021-10
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    1) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    2) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    3) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    4) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    5) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    6) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    7) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    8) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    9) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    10) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    11) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    12) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    13) Product Images from "Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal"

    Article Title: Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal

    Journal: bioRxiv

    doi: 10.1101/2021.03.15.435455

    Age- and/or sex-specific variation in (A) PMBC; (B) CD4 + cells; (C) CD4 + Tbet + cells; (D) CD4 + Gata3 + cells; (E) CD4 + RORγt + cells; (F) CD4 + FoxP3 + cells; (G) IFN-γ; (H) IL-4; (I) IL-17A; and (J) IL-10. Points show raw data, with green representing females and purple showing males. Large points connected by lines show estimates ±95%CI from a model where age was fitted as a factor (with 2 or 4 levels) and lines with shaded areas show estimates ±95%CI from models where age was fitted as a continuous variable; green lines represent females and purple lines males. For model details, see Tables S4 S5.
    Figure Legend Snippet: Age- and/or sex-specific variation in (A) PMBC; (B) CD4 + cells; (C) CD4 + Tbet + cells; (D) CD4 + Gata3 + cells; (E) CD4 + RORγt + cells; (F) CD4 + FoxP3 + cells; (G) IFN-γ; (H) IL-4; (I) IL-17A; and (J) IL-10. Points show raw data, with green representing females and purple showing males. Large points connected by lines show estimates ±95%CI from a model where age was fitted as a factor (with 2 or 4 levels) and lines with shaded areas show estimates ±95%CI from models where age was fitted as a continuous variable; green lines represent females and purple lines males. For model details, see Tables S4 S5.

    Techniques Used:

    14) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    15) Product Images from "Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal"

    Article Title: Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal

    Journal: bioRxiv

    doi: 10.1101/2021.03.15.435455

    Age- and/or sex-specific variation in (A) PMBC; (B) CD4 + cells; (C) CD4 + Tbet + cells; (D) CD4 + Gata3 + cells; (E) CD4 + RORγt + cells; (F) CD4 + FoxP3 + cells; (G) IFN-γ; (H) IL-4; (I) IL-17A; and (J) IL-10. Points show raw data, with green representing females and purple showing males. Large points connected by lines show estimates ±95%CI from a model where age was fitted as a factor (with 2 or 4 levels) and lines with shaded areas show estimates ±95%CI from models where age was fitted as a continuous variable; green lines represent females and purple lines males. For model details, see Tables S4 S5.
    Figure Legend Snippet: Age- and/or sex-specific variation in (A) PMBC; (B) CD4 + cells; (C) CD4 + Tbet + cells; (D) CD4 + Gata3 + cells; (E) CD4 + RORγt + cells; (F) CD4 + FoxP3 + cells; (G) IFN-γ; (H) IL-4; (I) IL-17A; and (J) IL-10. Points show raw data, with green representing females and purple showing males. Large points connected by lines show estimates ±95%CI from a model where age was fitted as a factor (with 2 or 4 levels) and lines with shaded areas show estimates ±95%CI from models where age was fitted as a continuous variable; green lines represent females and purple lines males. For model details, see Tables S4 S5.

    Techniques Used:

    16) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    17) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    18) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    19) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    20) Product Images from "Expansion, isolation and first characterization of bovine Th17 lymphocytes"

    Article Title: Expansion, isolation and first characterization of bovine Th17 lymphocytes

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52562-2

    Choice of culture medium for the expansion of Th17 cells. ( a ) Cell growth after 3, 6, 8 or 13 days in RPMI (+FCS and 2 ng/mL TGF-β1) or IMDM (+10% KnockOut Serum Replacement) with different concentrations of recombinant human TGF-β1. ( b ) Proportions of IL-17A, IFN-γ and double positive cells after 6 days of culture as a function of TGF-β1 concentration (0 to 5 ng/mL). Results are means from the cells of two cows. ( c ) Proportions of IL-17A+ cells after 6 and 13 days of culture as a function of TGF-β1 concentration (0 to 5 ng/mL). Results are means from the cells of two cows. ( d ) Comparison of cell growth in RPMI and X-VIVO™ 15 media with or without polarizing cytokines (40 ng/mL IL-6 and 2 ng/mL TGF-β1), after 3 and 6 days of culture. ( e ) Proportions of cells IL-17A+ and IFN-γ+ (ICS) with or without polarizing cytokines (40 ng/mL IL-6 and 2 ng/mL TGF-β1) after 6 days of culture. Results are means from two cows.
    Figure Legend Snippet: Choice of culture medium for the expansion of Th17 cells. ( a ) Cell growth after 3, 6, 8 or 13 days in RPMI (+FCS and 2 ng/mL TGF-β1) or IMDM (+10% KnockOut Serum Replacement) with different concentrations of recombinant human TGF-β1. ( b ) Proportions of IL-17A, IFN-γ and double positive cells after 6 days of culture as a function of TGF-β1 concentration (0 to 5 ng/mL). Results are means from the cells of two cows. ( c ) Proportions of IL-17A+ cells after 6 and 13 days of culture as a function of TGF-β1 concentration (0 to 5 ng/mL). Results are means from the cells of two cows. ( d ) Comparison of cell growth in RPMI and X-VIVO™ 15 media with or without polarizing cytokines (40 ng/mL IL-6 and 2 ng/mL TGF-β1), after 3 and 6 days of culture. ( e ) Proportions of cells IL-17A+ and IFN-γ+ (ICS) with or without polarizing cytokines (40 ng/mL IL-6 and 2 ng/mL TGF-β1) after 6 days of culture. Results are means from two cows.

    Techniques Used: Knock-Out, Recombinant, Concentration Assay

    Identification of IL-17A and IFN-γ producing bovine lymphocytes. PBMC were stimulated with PMA/ionomycin, cytokine secretion blocked with Brefeldin A before flow cytometry analysis. Debris were excluded by gating according to FSC/SSC, and after gating on singlet cells, dead cells were excluded by gating on live cells. The CD4+ cells were gated by taking into account the isotype control, and the production of IL-17A and IFN-γ was measured by intracellular labelling with specific antibodies. Results shown are from a representative experiment. FSC: forward scatter: SSC: side scatter.
    Figure Legend Snippet: Identification of IL-17A and IFN-γ producing bovine lymphocytes. PBMC were stimulated with PMA/ionomycin, cytokine secretion blocked with Brefeldin A before flow cytometry analysis. Debris were excluded by gating according to FSC/SSC, and after gating on singlet cells, dead cells were excluded by gating on live cells. The CD4+ cells were gated by taking into account the isotype control, and the production of IL-17A and IFN-γ was measured by intracellular labelling with specific antibodies. Results shown are from a representative experiment. FSC: forward scatter: SSC: side scatter.

    Techniques Used: Flow Cytometry, Cytometry

    Unambiguous identification of bovine Th17. ( a ) Secretion of cytokines by sorted Th17 cells. After sorting, the cells were stimulated with α-CD3 and α-CD28 and cultured for 6 days. Supernatant contents were analysed by ELISA. Median values (10 to 90 percentiles) from five cell preparations from three cows are shown. ( b ) Comparison of Th17 signature gene expression between IL-17A+ and IL-17A- cells. Heatmap shows expression intensity expressed as log2(fold-change) normalized relative to three reference genes (ACTB, PPIA and GAPDH). Expression is relative to the CD4+ subset of the corresponding cow at day 6 after positive selection using MACS® beads. Cells were cultured in X-VIVO™ 15 medium without polarizing cytokines for 6 days after an initial TCR stimulation and a partial (half) renewal of medium with addition of IL-2 on the third day. Results are from three cows (C1, C2, C3).
    Figure Legend Snippet: Unambiguous identification of bovine Th17. ( a ) Secretion of cytokines by sorted Th17 cells. After sorting, the cells were stimulated with α-CD3 and α-CD28 and cultured for 6 days. Supernatant contents were analysed by ELISA. Median values (10 to 90 percentiles) from five cell preparations from three cows are shown. ( b ) Comparison of Th17 signature gene expression between IL-17A+ and IL-17A- cells. Heatmap shows expression intensity expressed as log2(fold-change) normalized relative to three reference genes (ACTB, PPIA and GAPDH). Expression is relative to the CD4+ subset of the corresponding cow at day 6 after positive selection using MACS® beads. Cells were cultured in X-VIVO™ 15 medium without polarizing cytokines for 6 days after an initial TCR stimulation and a partial (half) renewal of medium with addition of IL-2 on the third day. Results are from three cows (C1, C2, C3).

    Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing, Selection, Magnetic Cell Separation

    Comparison of the Cytokine Secretion Assay with the cytokine surface Staining. ( a ) Gating strategy. At the end of the culture expansion step, the polarized cell populations were split and analyzed side-by-side by flow cytometry with Cytokine Secretion (left) and surface staining (right) assays for IL-17A secretion. The squares indicate the IL-17A+ cells sorting windows, the circles the IL-17A- sorting windows. ( b ) Proportions of cytokine-positive cells 8 days after sorting. Median values from two sorting experiments with cells of two cows are shown. ( c ) Numbers of IL-17A+ and IL-17A- cells at different times after sorting. Stars indicate stimulations with anti-CD3/CD28 antibodies, arrows the sampling of cells for freezing or RNA preparation. Values are from one sorting experiment with two cows. ( d ) Proportions of sorted IL-17A+ cells producing (ICS) IL-17A, IFN-γ or IL-22 at different times after sorting. Values are from one sorting experiment with two cows. e) Proportions of sorted IL-17A- cells producing (ICS) IL-17A, IFN-γ or IL-22 at different times after sorting. CSA: cytokine secretion assay; CSS: cytokine surface staining.
    Figure Legend Snippet: Comparison of the Cytokine Secretion Assay with the cytokine surface Staining. ( a ) Gating strategy. At the end of the culture expansion step, the polarized cell populations were split and analyzed side-by-side by flow cytometry with Cytokine Secretion (left) and surface staining (right) assays for IL-17A secretion. The squares indicate the IL-17A+ cells sorting windows, the circles the IL-17A- sorting windows. ( b ) Proportions of cytokine-positive cells 8 days after sorting. Median values from two sorting experiments with cells of two cows are shown. ( c ) Numbers of IL-17A+ and IL-17A- cells at different times after sorting. Stars indicate stimulations with anti-CD3/CD28 antibodies, arrows the sampling of cells for freezing or RNA preparation. Values are from one sorting experiment with two cows. ( d ) Proportions of sorted IL-17A+ cells producing (ICS) IL-17A, IFN-γ or IL-22 at different times after sorting. Values are from one sorting experiment with two cows. e) Proportions of sorted IL-17A- cells producing (ICS) IL-17A, IFN-γ or IL-22 at different times after sorting. CSA: cytokine secretion assay; CSS: cytokine surface staining.

    Techniques Used: Staining, Flow Cytometry, Cytometry, Sampling

    Maintenance of the IL-17A and IFN-γ phenotype of sorted and frozen-thawed cells upon subculture. ( a ) Sorted IL-17A+ cells stimulated with α-CD3/α-CD28 were cultured with IL-2 with or without the polarizing cytokines TGF-β1 and IL-6 for 9 days and analyzed by flow cytometry (ICS). Percentages of IL-17A+/IFN-γ+ double positive cells and of IL-17A-/IFN-g+ cells are shown. ( b ) Thawed IL-17A+ cells were cultured for 22 days and analyzed by ICS at days 12 and 22. Percentages of IL-17A+ cells (all: both IL-17A+/IFN-γ- and double positive cells) and IL-17A-/IFN-γ+ cells are shown. Results from two cows (C1 C2) are shown.
    Figure Legend Snippet: Maintenance of the IL-17A and IFN-γ phenotype of sorted and frozen-thawed cells upon subculture. ( a ) Sorted IL-17A+ cells stimulated with α-CD3/α-CD28 were cultured with IL-2 with or without the polarizing cytokines TGF-β1 and IL-6 for 9 days and analyzed by flow cytometry (ICS). Percentages of IL-17A+/IFN-γ+ double positive cells and of IL-17A-/IFN-g+ cells are shown. ( b ) Thawed IL-17A+ cells were cultured for 22 days and analyzed by ICS at days 12 and 22. Percentages of IL-17A+ cells (all: both IL-17A+/IFN-γ- and double positive cells) and IL-17A-/IFN-γ+ cells are shown. Results from two cows (C1 C2) are shown.

    Techniques Used: Cell Culture, Flow Cytometry, Cytometry

    Culture conditions for expansion of Th17 cells. ( a ) Number of cells after 6, 9 and 13 days of culture as a function of the concentration (µg/mL) of the coating antibodies to CD3 with or without 10 ng/ml recombinant human IL-2. Results are from a representative experiment. ( b ) Concentrations of IL-17A and IFN-γ under these culture conditions. ( c ) Proportion of cells producing IL-17A or IFN-γ (ICS) at day 6 of culture. Results are means from the cells of two cows. Percentages of IL-17A positive cells differed as a function of treatment (p = 0.034, one-way ANOVA), but not percentages of IFN-γ positive cells (p = 0.24, one-way ANOVA). Results with α-CD3 1 µg/mL are not shown because too few cells were available for staining.
    Figure Legend Snippet: Culture conditions for expansion of Th17 cells. ( a ) Number of cells after 6, 9 and 13 days of culture as a function of the concentration (µg/mL) of the coating antibodies to CD3 with or without 10 ng/ml recombinant human IL-2. Results are from a representative experiment. ( b ) Concentrations of IL-17A and IFN-γ under these culture conditions. ( c ) Proportion of cells producing IL-17A or IFN-γ (ICS) at day 6 of culture. Results are means from the cells of two cows. Percentages of IL-17A positive cells differed as a function of treatment (p = 0.034, one-way ANOVA), but not percentages of IFN-γ positive cells (p = 0.24, one-way ANOVA). Results with α-CD3 1 µg/mL are not shown because too few cells were available for staining.

    Techniques Used: Concentration Assay, Recombinant, Staining

    Efficiency of the monoclonal antibodies in the cytokine secretion assay. ( a ) Percentages of CD4+ T cells labelled by the capture complex with either the α-Cter or α-Nter monoclonals, and comparison with the percentages of IL-17A+ cells identified by ICS. Data from two cows (C1 C2) are shown, one per row. ( b ) Concentrations of IL-17A in culture supernatant before capture, at the end of the 3.5 h of stimulation. ( c ) Concentrations of IL-17A in culture supernatant at the end of the secretion step (after capture) in the presence of the complex capture with either one of the two monoclonal antibodies to IL-17A, an isotype control or the polyclonal antiserum to IL-17A, showing the efficiency of the capture of IL-17A as it is secreted. PI: PMA/ionomycin, PIB: PMA/ionomycin/brefeldin A. ICS: intracellular staining.
    Figure Legend Snippet: Efficiency of the monoclonal antibodies in the cytokine secretion assay. ( a ) Percentages of CD4+ T cells labelled by the capture complex with either the α-Cter or α-Nter monoclonals, and comparison with the percentages of IL-17A+ cells identified by ICS. Data from two cows (C1 C2) are shown, one per row. ( b ) Concentrations of IL-17A in culture supernatant before capture, at the end of the 3.5 h of stimulation. ( c ) Concentrations of IL-17A in culture supernatant at the end of the secretion step (after capture) in the presence of the complex capture with either one of the two monoclonal antibodies to IL-17A, an isotype control or the polyclonal antiserum to IL-17A, showing the efficiency of the capture of IL-17A as it is secreted. PI: PMA/ionomycin, PIB: PMA/ionomycin/brefeldin A. ICS: intracellular staining.

    Techniques Used: Staining

    Schematic representation of the procedure used for the isolation and expansion of Th17 cells. CD4+ cells were isolated from PBMC by magnetic sorting, expanded in a culture medium without serum in the presence of polarizing cytokines and TCR-stimulation, activated, labelled for surface IL-17A and sorted by flow cytometry. The sorted cells were expanded and maintained in culture for several weeks. The indicated timeline is kept in all the figures to make it clear at which step cells were assayed. Concentrations of cytokines were 10 ng/mL (IL-2), 40 ng/mL (IL-6) and 2 ng/mL (TGF-β1). D: day; CSA: cytokine secretion assay; CSS: cytokine surface staining. α-: antibody anti-.
    Figure Legend Snippet: Schematic representation of the procedure used for the isolation and expansion of Th17 cells. CD4+ cells were isolated from PBMC by magnetic sorting, expanded in a culture medium without serum in the presence of polarizing cytokines and TCR-stimulation, activated, labelled for surface IL-17A and sorted by flow cytometry. The sorted cells were expanded and maintained in culture for several weeks. The indicated timeline is kept in all the figures to make it clear at which step cells were assayed. Concentrations of cytokines were 10 ng/mL (IL-2), 40 ng/mL (IL-6) and 2 ng/mL (TGF-β1). D: day; CSA: cytokine secretion assay; CSS: cytokine surface staining. α-: antibody anti-.

    Techniques Used: Isolation, Flow Cytometry, Cytometry, Staining

    Schematic representation of the assays used for surface labelling of IL-17A secreting cells. In the cytokine secretion assay (CSA), after stimulation with PMA/ionomycin, the cells start to secrete cytokines for 3.5 h. Then the cells are allowed to bind the capture complex (Capture complex antibody staining) for 15 min before dilution and incubation for 1.5 h under agitation (New cytokine secretion step). The secreted cytokine is captured by antibodies to IL-17A that are maintained at the surface of the cell by antibodies to CD45. The capture complex comprises the two types of biotinylated antibodies linked by a streptavidin molecule. After washing, rabbit anti-bovine IL-17A antibodies are added that bind to the captured IL-17A (IL-17A detection labelling and revelation). Then the cells are washed and the binding of rabbit antibodies revealed with a secondary antibody conjugated to phycoerythrin. Alternatively, in the cytokine surface staining assay (CSS), after washing at the end of the 3.5 h stimulation, cells are incubated with rabbit antibodies to IL-17A, washed, and the binding of rabbit antibodies to surface-associated IL-17A is revealed with a secondary antibody conjugated to phycoerythrin.
    Figure Legend Snippet: Schematic representation of the assays used for surface labelling of IL-17A secreting cells. In the cytokine secretion assay (CSA), after stimulation with PMA/ionomycin, the cells start to secrete cytokines for 3.5 h. Then the cells are allowed to bind the capture complex (Capture complex antibody staining) for 15 min before dilution and incubation for 1.5 h under agitation (New cytokine secretion step). The secreted cytokine is captured by antibodies to IL-17A that are maintained at the surface of the cell by antibodies to CD45. The capture complex comprises the two types of biotinylated antibodies linked by a streptavidin molecule. After washing, rabbit anti-bovine IL-17A antibodies are added that bind to the captured IL-17A (IL-17A detection labelling and revelation). Then the cells are washed and the binding of rabbit antibodies revealed with a secondary antibody conjugated to phycoerythrin. Alternatively, in the cytokine surface staining assay (CSS), after washing at the end of the 3.5 h stimulation, cells are incubated with rabbit antibodies to IL-17A, washed, and the binding of rabbit antibodies to surface-associated IL-17A is revealed with a secondary antibody conjugated to phycoerythrin.

    Techniques Used: Staining, Incubation, Binding Assay

    21) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    22) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    23) Product Images from "T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland"

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063471

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.
    Figure Legend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Techniques Used: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p
    Figure Legend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    24) Product Images from "Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle"

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    Journal: Veterinary Research

    doi: 10.1186/s13567-016-0329-x

    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
    Figure Legend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Techniques Used: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P
    Figure Legend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Techniques Used: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.
    Figure Legend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Techniques Used: Western Blot, Translocation Assay, Positive Control

    25) Product Images from "Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal"

    Article Title: Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal

    Journal: bioRxiv

    doi: 10.1101/2021.03.15.435455

    Age- and/or sex-specific variation in (A) PMBC; (B) CD4 + cells; (C) CD4 + Tbet + cells; (D) CD4 + Gata3 + cells; (E) CD4 + RORγt + cells; (F) CD4 + FoxP3 + cells; (G) IFN-γ; (H) IL-4; (I) IL-17A; and (J) IL-10. Points show raw data, with green representing females and purple showing males. Large points connected by lines show estimates ±95%CI from a model where age was fitted as a factor (with 2 or 4 levels) and lines with shaded areas show estimates ±95%CI from models where age was fitted as a continuous variable; green lines represent females and purple lines males. For model details, see Tables S4 S5.
    Figure Legend Snippet: Age- and/or sex-specific variation in (A) PMBC; (B) CD4 + cells; (C) CD4 + Tbet + cells; (D) CD4 + Gata3 + cells; (E) CD4 + RORγt + cells; (F) CD4 + FoxP3 + cells; (G) IFN-γ; (H) IL-4; (I) IL-17A; and (J) IL-10. Points show raw data, with green representing females and purple showing males. Large points connected by lines show estimates ±95%CI from a model where age was fitted as a factor (with 2 or 4 levels) and lines with shaded areas show estimates ±95%CI from models where age was fitted as a continuous variable; green lines represent females and purple lines males. For model details, see Tables S4 S5.

    Techniques Used:

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    Article Snippet: .. Cytokine ELISA The secretion of IL-17A, IL-10, IFN-γ and IL-22 in cell-free supernatants was measured using commercial ELISA kits according to the manufacturer’s guidelines (IL-17A and IFN-γ, Kingfisher biotech; IL-10, Life Technologies; IL-22, Sigma-Aldrich). ..

    Article Title: Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves
    Article Snippet: .. The concentration of cytokines in cell culture supernatants was determined using bovine commercial ELISA kits for IFNγ (intra-assay coefficient of variation [CV] 3.6% and inter-assay CV 6.1%), interleukin ( IL)-17A (intra-assay CV 6.7% and inter-assay CV 7.6%), IL-6 (intra-assay CV 4.1% and inter-assay CV 5.0%), and TNFα (intra-assay CV 4.3% and inter-assay CV 17.0%) from Kingfisher Biotech, Inc. Minneapolis, MN. ..

    Concentration Assay:

    Article Title: Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves
    Article Snippet: .. The concentration of cytokines in cell culture supernatants was determined using bovine commercial ELISA kits for IFNγ (intra-assay coefficient of variation [CV] 3.6% and inter-assay CV 6.1%), interleukin ( IL)-17A (intra-assay CV 6.7% and inter-assay CV 7.6%), IL-6 (intra-assay CV 4.1% and inter-assay CV 5.0%), and TNFα (intra-assay CV 4.3% and inter-assay CV 17.0%) from Kingfisher Biotech, Inc. Minneapolis, MN. ..

    Cell Culture:

    Article Title: Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves
    Article Snippet: .. The concentration of cytokines in cell culture supernatants was determined using bovine commercial ELISA kits for IFNγ (intra-assay coefficient of variation [CV] 3.6% and inter-assay CV 6.1%), interleukin ( IL)-17A (intra-assay CV 6.7% and inter-assay CV 7.6%), IL-6 (intra-assay CV 4.1% and inter-assay CV 5.0%), and TNFα (intra-assay CV 4.3% and inter-assay CV 17.0%) from Kingfisher Biotech, Inc. Minneapolis, MN. ..

    Intra Assay:

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    Article Snippet: .. The concentration of cytokines in cell culture supernatants was determined using bovine commercial ELISA kits for IFNγ (intra-assay coefficient of variation [CV] 3.6% and inter-assay CV 6.1%), interleukin ( IL)-17A (intra-assay CV 6.7% and inter-assay CV 7.6%), IL-6 (intra-assay CV 4.1% and inter-assay CV 5.0%), and TNFα (intra-assay CV 4.3% and inter-assay CV 17.0%) from Kingfisher Biotech, Inc. Minneapolis, MN. ..

    Inter Assay:

    Article Title: Supplementing a Saccharomyces cerevisiae fermentation product modulates innate immune function and ameliorates bovine respiratory syncytial virus infection in neonatal calves
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    Kingfisher Biotech il 17a
    Effect of dexamethasone on LPS-, Pam3CSK4- and <t>IL-17A-induced</t> up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P
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    Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Journal: Veterinary Research

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    doi: 10.1186/s13567-016-0329-x

    Figure Lengend Snippet: Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10 −6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH ( P

    Article Snippet: Finally, NF-κB p65 was detected in the nuclear fraction of bTEC stimulated for 16 h with 316 ng/mL IL-17A, whereas treatment with 10 µM CAPE prior to stimulation with IL-17A partially inhibited this effect (Figure C).

    Techniques: Expressing

    Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Journal: Veterinary Research

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    doi: 10.1186/s13567-016-0329-x

    Figure Lengend Snippet: Effect of the NF-κB inhibitor caffeic acid phenylester (CAPE) on induction of TAP gene expression by LPS, Pam3CSK4 and IL-17A. Stimulation of bovine tracheal epithelial cells (bTEC) with 0.1 µg/mL LPS (panel A ), 1 µg/mL Pam3CSK4 (panel B ), or 316 ng/mL IL-17A (panel C ) induced significantly greater TAP gene expression than in non-stimulated cells (NS). Pre-treatment with CAPE for 2 h before stimulation inhibited each of these responses. Asterisks indicate significant effects of CAPE treatment compared to no CAPE, P

    Article Snippet: Finally, NF-κB p65 was detected in the nuclear fraction of bTEC stimulated for 16 h with 316 ng/mL IL-17A, whereas treatment with 10 µM CAPE prior to stimulation with IL-17A partially inhibited this effect (Figure C).

    Techniques: Expressing

    Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Journal: Veterinary Research

    Article Title: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle

    doi: 10.1186/s13567-016-0329-x

    Figure Lengend Snippet: Western analysis of NF-kB p65 in nuclear and cytoplasmic extracts of bTEC stimulated with LPS, Pam3CSK4 or IL-17A. NF-kB p65 was localized to the cytoplasm of non-stimulated cells (NS), but showed translocation to the nucleus following treatment of the cells with either LPS (panel A ), Pam3CSK4 (Pam, panel B ) or IL-17A (panel C ). However, pre-treating the cells with the NF-κB inhibitor CAPE (10 μM) prior to exposure to agonists fully abrogated nuclear translocation of NF-kB p65 in the studies involving LPS and Pam3CSK4 (panels A and B ), and partially in the studies involving IL-17A (panel C ). Pos control: positive control, NF-kB p65. The findings were similar when the experiments were repeated using cells from different animals.

    Article Snippet: Finally, NF-κB p65 was detected in the nuclear fraction of bTEC stimulated for 16 h with 316 ng/mL IL-17A, whereas treatment with 10 µM CAPE prior to stimulation with IL-17A partially inhibited this effect (Figure C).

    Techniques: Western Blot, Translocation Assay, Positive Control

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Journal: PLoS ONE

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    doi: 10.1371/journal.pone.0063471

    Figure Lengend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Article Snippet: A circumstantial evidence of IL-17A involvement was the chemokine and self-defense gene expression profiles found in milk and mammary tissue, which correspond to the expected response of stromal cells (epithelial and fibroblastic) to IL-17A .

    Techniques: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Journal: PLoS ONE

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    doi: 10.1371/journal.pone.0063471

    Figure Lengend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Article Snippet: A circumstantial evidence of IL-17A involvement was the chemokine and self-defense gene expression profiles found in milk and mammary tissue, which correspond to the expected response of stromal cells (epithelial and fibroblastic) to IL-17A .

    Techniques: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Journal: PLoS ONE

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    doi: 10.1371/journal.pone.0063471

    Figure Lengend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Article Snippet: A circumstantial evidence of IL-17A involvement was the chemokine and self-defense gene expression profiles found in milk and mammary tissue, which correspond to the expected response of stromal cells (epithelial and fibroblastic) to IL-17A .

    Techniques: Enzyme-linked Immunosorbent Assay