il 12 elisa kit  (Boster Bio)

Journal: Frontiers in Immunology

Article Title: Marijuana-Derived Cannabinoids Trigger a CB2/PI3K Axis of Suppression of the Innate Response to Oral Pathogens

doi: 10.3389/fimmu.2019.02288

Figure Lengend Snippet: Phytocannabinoids alter the monocytic cytokine release profile in response to oral bacteria. Control and CBD (2 h) pre-exposed monocytes were stimulated, or not, with LPS or P. gingivalis and cytokine [ (A) TNF, (B) IL-6, (C) IL-12 p40, (D) IL-8, (E) IL-10] release measured in 20 h cell-free supernatants by ELISA. Similar data was found upon F. alocis and T. denticola stimulation ( data not shown ). Similar phenomenon were also apparent with two the other phytocannabinoids tested, CBN and THC ( data not shown ). Data represent the mean ± SD of three biological replicates with paired data-points analyzed by t -test. */**/*** p ≤ 0.05/0.01/0.001.

Article Snippet: Cytokine ELISA kits (IL-6, IL-8, IL-10, TNF [TNF-α]) were purchased from eBioscience (San Diego, CA) while IL-12 p40 ELISA kits came from Boster Immunoleader (Pleasanton, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: ‘Psoriasis 1’ reduces psoriasis-like skin inflammation by inhibiting the VDR-mediated nuclear NF-κB and STAT signaling pathways

doi: 10.3892/mmr.2018.9262

Figure Lengend Snippet: ‘Psoriasis 1’ downregulates the expression of TNF-α, IFN-γ, IL-22, IL-17C, IL-1β and IL-4, and upregulates the expression of 25HVD3 in TNF-α-induced psoriatic models. The concentrations of (A) TNF-α, (B) 25HVD3, (C) IFN-γ, (D) IL-22, (E) IL-17C, (F) IL-1β and (G) IL-4 were detected by ELISA. ***P

Article Snippet: The ELISA kits were IL-1β ELISA kit (EK0392), TNF-α ELISA kit (k EK0525), IL-4 ELISA kit (EK0404), IL-17C ELISA kit (EK0789), IL-22 ELISA kit (EK0933), INF-γ ELISA kit (EK0373; all from Wuhan Boster Biological Technology, Ltd., Wuhan, China), and 25HVD3 ELISA kit (CSB-E08097h; Cusabio Technology LLC, Houston, TX, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: ‘Psoriasis 1’ reduces psoriasis-like skin inflammation by inhibiting the VDR-mediated nuclear NF-κB and STAT signaling pathways

doi: 10.3892/mmr.2018.9262

Figure Lengend Snippet: Silencing VDR decreases TNF-α, IFN-γ, IL-22, IL-17C, IL-1β and IL-4 expression levels, and increases the 25HVD3 expression level in TNF-α-induced HaCaT cells with stably silenced VDR. The concentrations of (A) TNF-α, (B) 25HVD3, (C) IFN-γ, (D) IL-22, (E) IL-17C, (F) IL-1β and (G) IL-4 were detected by ELISA. ***P

Article Snippet: The ELISA kits were IL-1β ELISA kit (EK0392), TNF-α ELISA kit (k EK0525), IL-4 ELISA kit (EK0404), IL-17C ELISA kit (EK0789), IL-22 ELISA kit (EK0933), INF-γ ELISA kit (EK0373; all from Wuhan Boster Biological Technology, Ltd., Wuhan, China), and 25HVD3 ELISA kit (CSB-E08097h; Cusabio Technology LLC, Houston, TX, USA).

Techniques: Expressing, Stable Transfection, Enzyme-linked Immunosorbent Assay

Journal: Oncotarget

Article Title: Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells

doi:

Figure Lengend Snippet: Secretion of IFN-γ and IL-12 from DCs under different stimulations Secretion of IFN-γ A. and IL-12 B. from DCs after incubation with ALA-PDT-treated PECA cells was quantified in cell culture supernatants using ELISA assay, with or without HSP70, HMGB1 or CRT neutralizing antibodies in the co-incubation medium. Unstimulated, immature DCs were used for negative control and DCs incubated with LPS were used for positive control. ALA-PDT-treated PECA cells induced the secretion of IFN-γ and IL-12 markedly higher than that induced by untreated PECA cells ( p

Article Snippet: In addition, we used mouse HSP70 ELISA Kit (ENEO, USA), mouse HMGB1 ELISA Kit (SHINO-TEST, Japan), mouse IFN-γ and IL-12 ELISA Kit (Boster, China), rabbit anti-Mouse CD80-PE, rabbit anti-Mouse CD86-PE, rabbit anti-Mouse MHC-II-PE, and rat IgG2a K Isotype Control PE (Biolegend, USA).

Techniques: Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control

Journal: Pharmaceutical Biology

Article Title: β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice

doi: 10.1080/13880209.2019.1577461

Figure Lengend Snippet: BS treatment repressed collagen-induced arthritis, collagen-specific antibodies and pro-inflammatory cytokine production in mice. (A) Timeline of animal experiment. (B) The swelling of hind paw was measured every three days using a vernier caliper. (C) The H E staining of hind paws. (D) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (E) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were collected from two individual experiments, and shown as mean and SEM. N = 6–8. * p

Article Snippet: Mouse IL-1β, IL-6, IL-12 and IL-10 ELISA kits were purchased from BOSTER Biological Technology Co. Ltd (Wuhan, China).

Techniques: Mouse Assay, Staining, Enzyme-linked Immunosorbent Assay

Journal: Pharmaceutical Biology

Article Title: β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice

doi: 10.1080/13880209.2019.1577461

Figure Lengend Snippet: IL-10 was critical for the anti-inflammatory response induced by BS treatment and BS-BMDMs transfer. The CIA induction, BS treatment and BS-BMDMs transfer were performed as above described. 100 μg IL-10 antibody was administered every 3 d and start on day 0. (A, E) Timeline of animal experiment. (B, F) The swelling of hind paw was measured every three days using a vernier caliper. (C, G) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (D, H) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were shown as mean and SEM. N = 5. * p

Article Snippet: Mouse IL-1β, IL-6, IL-12 and IL-10 ELISA kits were purchased from BOSTER Biological Technology Co. Ltd (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Pharmaceutical Biology

Article Title: β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice

doi: 10.1080/13880209.2019.1577461

Figure Lengend Snippet: BS-treated BMDMs alleviated collagen-induced arthritis in mice. The CIA was induced as above described. (A) Timeline of animal experiment. 2 × 10 6 BS-BMDMs or control BMDMs were transferred through tail vein at day −1. (B) The swelling of hind paw was measured every 3 d using a Vernier caliper. (C) The H E staining of hind paws. (D) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (E) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were collected from two individual experiments, and shown as mean and SEM. N = 5. * p

Article Snippet: Mouse IL-1β, IL-6, IL-12 and IL-10 ELISA kits were purchased from BOSTER Biological Technology Co. Ltd (Wuhan, China).

Techniques: Mouse Assay, Staining, Enzyme-linked Immunosorbent Assay