il 10 elisa kits  (Boster Bio)


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    Structured Review

    Boster Bio il 10 elisa kits
    BS treatment repressed collagen-induced arthritis, collagen-specific antibodies and pro-inflammatory cytokine production in mice. (A) Timeline of animal experiment. (B) The swelling of hind paw was measured every three days using a vernier caliper. (C) The H E staining of hind paws. (D) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by <t>ELISA</t> at a 1:100 dilution of serum. (E) The levels of serum IL-1β, IL-6, IL-12 and <t>IL-10</t> were measured by ELISA. Data were collected from two individual experiments, and shown as mean and SEM. N = 6–8. * p
    Il 10 Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 5 article reviews
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    il 10 elisa kits - by Bioz Stars, 2022-08
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    Images

    1) Product Images from "β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice"

    Article Title: β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice

    Journal: Pharmaceutical Biology

    doi: 10.1080/13880209.2019.1577461

    BS treatment repressed collagen-induced arthritis, collagen-specific antibodies and pro-inflammatory cytokine production in mice. (A) Timeline of animal experiment. (B) The swelling of hind paw was measured every three days using a vernier caliper. (C) The H E staining of hind paws. (D) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (E) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were collected from two individual experiments, and shown as mean and SEM. N = 6–8. * p
    Figure Legend Snippet: BS treatment repressed collagen-induced arthritis, collagen-specific antibodies and pro-inflammatory cytokine production in mice. (A) Timeline of animal experiment. (B) The swelling of hind paw was measured every three days using a vernier caliper. (C) The H E staining of hind paws. (D) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (E) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were collected from two individual experiments, and shown as mean and SEM. N = 6–8. * p

    Techniques Used: Mouse Assay, Staining, Enzyme-linked Immunosorbent Assay

    IL-10 was critical for the anti-inflammatory response induced by BS treatment and BS-BMDMs transfer. The CIA induction, BS treatment and BS-BMDMs transfer were performed as above described. 100 μg IL-10 antibody was administered every 3 d and start on day 0. (A, E) Timeline of animal experiment. (B, F) The swelling of hind paw was measured every three days using a vernier caliper. (C, G) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (D, H) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were shown as mean and SEM. N = 5. * p
    Figure Legend Snippet: IL-10 was critical for the anti-inflammatory response induced by BS treatment and BS-BMDMs transfer. The CIA induction, BS treatment and BS-BMDMs transfer were performed as above described. 100 μg IL-10 antibody was administered every 3 d and start on day 0. (A, E) Timeline of animal experiment. (B, F) The swelling of hind paw was measured every three days using a vernier caliper. (C, G) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (D, H) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were shown as mean and SEM. N = 5. * p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    BS-treated BMDMs alleviated collagen-induced arthritis in mice. The CIA was induced as above described. (A) Timeline of animal experiment. 2 × 10 6 BS-BMDMs or control BMDMs were transferred through tail vein at day −1. (B) The swelling of hind paw was measured every 3 d using a Vernier caliper. (C) The H E staining of hind paws. (D) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (E) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were collected from two individual experiments, and shown as mean and SEM. N = 5. * p
    Figure Legend Snippet: BS-treated BMDMs alleviated collagen-induced arthritis in mice. The CIA was induced as above described. (A) Timeline of animal experiment. 2 × 10 6 BS-BMDMs or control BMDMs were transferred through tail vein at day −1. (B) The swelling of hind paw was measured every 3 d using a Vernier caliper. (C) The H E staining of hind paws. (D) The collagen-specific IgG, IgG1 and IgG2c antibodies were detected by ELISA at a 1:100 dilution of serum. (E) The levels of serum IL-1β, IL-6, IL-12 and IL-10 were measured by ELISA. Data were collected from two individual experiments, and shown as mean and SEM. N = 5. * p

    Techniques Used: Mouse Assay, Staining, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Neuroprotective Effects of Safflower Flavonoid Extract in 6-Hydroxydopamine-Induced Model of Parkinson’s Disease May Be Related to its Anti-Inflammatory Action"

    Article Title: Neuroprotective Effects of Safflower Flavonoid Extract in 6-Hydroxydopamine-Induced Model of Parkinson’s Disease May Be Related to its Anti-Inflammatory Action

    Journal: Molecules

    doi: 10.3390/molecules25215206

    Effect of K3R and AYB in a neuron-astrocyte coculture system. ( A – C ) The coculture (neuron + astrocyte (AS)) system reduced apoptosis rate of neurons as compared with single-culture (neuron). Representative photographs of nuclear bodies stained with Hoechst 33342, apoptotic cell nuclei were indicated by the arrows; ( B – D ), Effect of K3R and SYB on the neuronal apoptosis rate in the coculture system (bar = 50 μm); ( E ) Effects of K3R and AYB on the neuronal viability in the coculture system damaged by 6-OHDA; ( F ) Contents of IL-1β and IL-10 in the co-culture medium. n = 6. # p
    Figure Legend Snippet: Effect of K3R and AYB in a neuron-astrocyte coculture system. ( A – C ) The coculture (neuron + astrocyte (AS)) system reduced apoptosis rate of neurons as compared with single-culture (neuron). Representative photographs of nuclear bodies stained with Hoechst 33342, apoptotic cell nuclei were indicated by the arrows; ( B – D ), Effect of K3R and SYB on the neuronal apoptosis rate in the coculture system (bar = 50 μm); ( E ) Effects of K3R and AYB on the neuronal viability in the coculture system damaged by 6-OHDA; ( F ) Contents of IL-1β and IL-10 in the co-culture medium. n = 6. # p

    Techniques Used: Staining, Co-Culture Assay

    Effect of SAFE on inflammation. ( A ) Representative photographs of co-staining (model group, coronal section) of TH (red), glial fibrillary acidic protein (GFAP, green), and DAPI (blue) in the SN; ( B ) Enlarged views of the indicated regions in (A); ( C ) Representative images of GFAP-stained cells in the SN of mice in each group of animals (bar = 50 μm); ( D ) Fluorescence intensity levels of GFAP-stained cells in the lesioned side of the SN in each group, n = 4; ( E ) Representative Western blot and quantitative analysis of the relative intensity of Iba-1/GAPDH in the striatum, n = 3; ( F ) Plasma contents of TNF-α ( n = 7), IL-6 ( n = 8), IL-10 ( n = 5), and IL-1β ( n = 4). Cytokine concentrations were determined using ELISA kits; ( G ) Representative Western blot images and quantitative analysis of the relative intensity of NLRP3/GAPDH and caspase 1/GAPDH, n = 3. # p
    Figure Legend Snippet: Effect of SAFE on inflammation. ( A ) Representative photographs of co-staining (model group, coronal section) of TH (red), glial fibrillary acidic protein (GFAP, green), and DAPI (blue) in the SN; ( B ) Enlarged views of the indicated regions in (A); ( C ) Representative images of GFAP-stained cells in the SN of mice in each group of animals (bar = 50 μm); ( D ) Fluorescence intensity levels of GFAP-stained cells in the lesioned side of the SN in each group, n = 4; ( E ) Representative Western blot and quantitative analysis of the relative intensity of Iba-1/GAPDH in the striatum, n = 3; ( F ) Plasma contents of TNF-α ( n = 7), IL-6 ( n = 8), IL-10 ( n = 5), and IL-1β ( n = 4). Cytokine concentrations were determined using ELISA kits; ( G ) Representative Western blot images and quantitative analysis of the relative intensity of NLRP3/GAPDH and caspase 1/GAPDH, n = 3. # p

    Techniques Used: Staining, Mouse Assay, Fluorescence, Western Blot, Enzyme-linked Immunosorbent Assay

    3) Product Images from "The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis"

    Article Title: The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis

    Journal: Molecules

    doi: 10.3390/molecules26144149

    LA protected mice against dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) and attenuated colonic inflammation. ( A ) Body weight of mice over the course of 14 days. ( B ) Disease activity index (DAI) score of each mouse on day 14. ( C ) Hematoxylin and eosin (H E) scores. ( D ) Photomicrographs of H E staining (200×). ( E ) Levels of TNF-α, IL-6, IL-1β, and IL-10 in colon tissue. Data are expressed as mean ±SD ( n = 6); # p
    Figure Legend Snippet: LA protected mice against dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) and attenuated colonic inflammation. ( A ) Body weight of mice over the course of 14 days. ( B ) Disease activity index (DAI) score of each mouse on day 14. ( C ) Hematoxylin and eosin (H E) scores. ( D ) Photomicrographs of H E staining (200×). ( E ) Levels of TNF-α, IL-6, IL-1β, and IL-10 in colon tissue. Data are expressed as mean ±SD ( n = 6); # p

    Techniques Used: Mouse Assay, Activity Assay, Staining

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    Boster Bio mouse il 10 elisa kit picokine
    Effects of VitD 3 on intestinal permeability and inflammatory cytokine secretion in serum of mice. (a) DAO activity in serum. (b) D-LA concentration in serum. (c) TNF- α concentration in serum. (d) IL-1 β concentration in serum. (e) IL-6 concentration in serum. (f) <t>IL-10</t> concentration in serum. All data were presented as mean ± SEM ( n = 10). ∗ P
    Mouse Il 10 Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 10 elisa kit picokine/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    94
    Boster Bio mouse cytokines
    pRCPS effect on serum <t>cytokines</t> in immunosuppressed mice. After 24 h of the last treatment, serum samples were collected and the ( A ) IFN-γ, ( B ) IL-4, ( C ) IL-2, ( D ) IL-5, ( E ) IL-6, and ( F ) IL-10 were determined by ELISA. Results are presented as the mean ±SD ( n = 10). Bars marked with different letters (a–d) indicate differences that are statistically significant ( p
    Mouse Cytokines, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Boster Bio il 10
    Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase <t>IL-10</t> expression of rats with nucleus pulposus (NP) (** P
    Il 10, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 10/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 10 - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effects of VitD 3 on intestinal permeability and inflammatory cytokine secretion in serum of mice. (a) DAO activity in serum. (b) D-LA concentration in serum. (c) TNF- α concentration in serum. (d) IL-1 β concentration in serum. (e) IL-6 concentration in serum. (f) IL-10 concentration in serum. All data were presented as mean ± SEM ( n = 10). ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Vitamin D3 Protects Mice from Diquat-Induced Oxidative Stress through the NF-κB/Nrf2/HO-1 Signaling Pathway

    doi: 10.1155/2021/6776956

    Figure Lengend Snippet: Effects of VitD 3 on intestinal permeability and inflammatory cytokine secretion in serum of mice. (a) DAO activity in serum. (b) D-LA concentration in serum. (c) TNF- α concentration in serum. (d) IL-1 β concentration in serum. (e) IL-6 concentration in serum. (f) IL-10 concentration in serum. All data were presented as mean ± SEM ( n = 10). ∗ P

    Article Snippet: The kits to detect the concentration of TNF-α (Catalog number EK0527), IL-1β (Catalog number EK0394), IL-6 (Catalog number EK0411), and IL-10 (Catalog number EK0417) in serum were purchased from Boster (Wuhan, China).

    Techniques: Permeability, Mouse Assay, Activity Assay, Concentration Assay

    pRCPS effect on serum cytokines in immunosuppressed mice. After 24 h of the last treatment, serum samples were collected and the ( A ) IFN-γ, ( B ) IL-4, ( C ) IL-2, ( D ) IL-5, ( E ) IL-6, and ( F ) IL-10 were determined by ELISA. Results are presented as the mean ±SD ( n = 10). Bars marked with different letters (a–d) indicate differences that are statistically significant ( p

    Journal: Molecules

    Article Title: Immunomodulatory Effects of Phosphorylated Radix Cyathulae officinalis Polysaccharides in Immunosuppressed Mice

    doi: 10.3390/molecules24224150

    Figure Lengend Snippet: pRCPS effect on serum cytokines in immunosuppressed mice. After 24 h of the last treatment, serum samples were collected and the ( A ) IFN-γ, ( B ) IL-4, ( C ) IL-2, ( D ) IL-5, ( E ) IL-6, and ( F ) IL-10 were determined by ELISA. Results are presented as the mean ±SD ( n = 10). Bars marked with different letters (a–d) indicate differences that are statistically significant ( p

    Article Snippet: Propidium iodide, ConA (concanavalin A), media (RPMI-1640), and ELISA kits for mouse cytokines (IL-2, -4, -5, -6, -10, and IFN-γ) were purchased from Wuhan Boster Biological Technology, (Hubei, China). l -glutamine, benzylpenicillin and streptomycin (both 100 IU/ mL), streptomycin (100 μg/mL), fetal bovine serum, and penicillin (100 U/mL) were procured from Gibco Chemical Co. (Saint Louis, Missouri, USA).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase IL-10 expression of rats with nucleus pulposus (NP) (** P

    Journal: The Korean Journal of Pain

    Article Title: Toll-like receptor 4/nuclear factor-kappa B pathway is involved in radicular pain by encouraging spinal microglia activation and inflammatory response in a rat model of lumbar disc herniation

    doi: 10.3344/kjp.2021.34.1.47

    Figure Lengend Snippet: Both TAK242 (TAK) and pyrrolidinedithiocarbamic acid (PDTC) reduce spinal expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), increase IL-10 expression of rats with nucleus pulposus (NP) (** P

    Article Snippet: Enzyme linked immunosorbent assay (ELISA) The dorsal quadrants of the L4-L5 spinal dorsal horn were rapidly harvested and homogenized in PBS followed by centrifugation at 4°C for 15 min at 14,000 × g. The supernatants were collected to detect the concentrations of TNF-α, IL-1β, IL-6, and IL-10 using corresponding ELISA kits (cat no. EK0526, EK0393, EK0412, EK0418; Boster Biological Technology).

    Techniques: Expressing

    (a) Effect of berberine on serum IL-1, TNF- α , and IL-4 levels. (b) Effect of berberine on serum IL-6, IL-8, IL-10, and IL-12/P70 levels. (c) IL-1, TNF- α , and IL-4 in colon tissue (immunohistochemistry, x200). (d) Expressions of IL-1, TNF- α , and IL-4 in colon tissue ( ∗ compared with normal control group, P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Exploring the Mechanism of Berberine Intervention in Ulcerative Colitis from the Perspective of Inflammation and Immunity Based on Systemic Pharmacology

    doi: 10.1155/2021/9970240

    Figure Lengend Snippet: (a) Effect of berberine on serum IL-1, TNF- α , and IL-4 levels. (b) Effect of berberine on serum IL-6, IL-8, IL-10, and IL-12/P70 levels. (c) IL-1, TNF- α , and IL-4 in colon tissue (immunohistochemistry, x200). (d) Expressions of IL-1, TNF- α , and IL-4 in colon tissue ( ∗ compared with normal control group, P

    Article Snippet: Experimental Drugs, Reagents, and Instruments The experimental drugs, reagents, and instruments are as follows: berberine hydrochloride (Shanghai Xinyi Tianping Pharmaceutical Co., Ltd., National Medicine Standard H31021444, 0.1 g/tablet), dextran sulfate sodium salt (DSS) (Sigma Inc., batch number 31404-5G-F), H & E staining kit (Wuhan Boster Biological Technology Co., Ltd., batch number AR1180), interleukin-1β (IL-1β ), tumor necrosis factor-α (TNF-α ), IL-4 ELISA kit (Ambion company, catalog numbers xyR013, xyR017, and xyR097), IL-8 ELISA kit (Nanjing Senbega Biotechnology Co., Ltd., batch number: SBJ-R0700), Rat IL-6 ELISA kit (Cat. No. EK0412), Rat IL-12 (P70) ELISA kit (Cat. No. EK1652), Rat IL-10 ELISA kit (Cat. No. EK0418) (Wuhan Boster Bioengineering Co., Ltd.), rabbit anti-mouse IL-1β , TNF-α, IL-4 antibodies (Beijing Boaosen Biotechnology Co., Ltd., batch numbers bs-10859R, bs-10802R, and bs-1740R), horseradish enzyme-labeled goat anti-rabbit IgG secondary antibody (Beijing Zhongshan Jinqiao Biotechnology Co., Ltd., batch number ZB1208), rotary microtome (Leica RM2235 rotary microtome), optical scanning microscope (Zeiss Optical Instruments Beijing Branch), and microplate reader (Rui Xuan Electronic Technology Co., Ltd.).

    Techniques: Immunohistochemistry