Journal: mSphere

Article Title: The Pseudomonas aeruginosa effector protein TesG regulates alternative activation of macrophages through NLRC5

doi: 10.1128/msphere.00681-25

Figure Lengend Snippet: TesG regulates the polarization of macrophages through known functions of NLRC5. ( A ) iBMDMs were treated overnight with either TesG protein (10 µg/mL) alone or in combination with NLRC5 pathway inhibitors (5 µM Ac-YVAD-cmk, caspase-1 inhibitor; 1 µM peficitinib, JAK inhibitor; and 0.1 µM IKK16, IKK-2 inhibitor), and macrophage polarization was analyzed by flow cytometry. Data are presented as mean ± SEM of three to four biological replicates, with statistical significance determined by one-way ANOVA with Tukey’s multiple comparison test: * P < 0.05; ** P < 0.01; *** P < 0.001; and ns, not significant.

Article Snippet: Adherent cells in 12-well plates were incubated overnight with TesG (10 μg/mL) together with DMSO (vehicle control) or in combination with one of the following inhibitors: Ac-YVAD-cmk (5 μM), peficitinib (1 μM), or IKK16 (0.1 μM) (all from MedChemExpress).

Techniques: Flow Cytometry, Comparison