iii 8 dna  (Thermo Fisher)


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    Name:
    pBR322 DNA BsuRI HaeIII Marker
    Description:
    Thermo Scientific pBR322 DNA BsuRI HaeIII Marker is recommended for sizing and approximate quantification of small linear double stranded DNA fragments in agarose and non denaturing polyacrylamide gels pBR322 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme then purified and dissolved in storage buffer The DNA fragments contain blunt or sticky ends depending on the restriction enzyme used for the marker s preparationThe marker can be labeled radioactively with T4 Polynucleotide Kinase see Resources for protocol Highlights• Sizing and approximate quantification of small DNA fragments• Sharp bands• Supplied with loading dye for sample DNANote• Bands at 587 540 and 458 bp form an anomalous pattern on polyacrylamide gels • The shortest fragments oblique are not visible in standard electrophoresis • Fragment lengths are predicted by computer analysis of the respective DNA sequences
    Catalog Number:
    SM0271
    Price:
    None
    Category:
    Standards Ladders Controls
    Applications:
    Agarose Gel Electrophoresis|DNA & RNA Purification & Analysis|Nucleic Acid Gel Electrophoresis & Blotting
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    Structured Review

    Thermo Fisher iii 8 dna
    Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual <t>III-8.</t> Solid-shaded large
    Thermo Scientific pBR322 DNA BsuRI HaeIII Marker is recommended for sizing and approximate quantification of small linear double stranded DNA fragments in agarose and non denaturing polyacrylamide gels pBR322 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme then purified and dissolved in storage buffer The DNA fragments contain blunt or sticky ends depending on the restriction enzyme used for the marker s preparationThe marker can be labeled radioactively with T4 Polynucleotide Kinase see Resources for protocol Highlights• Sizing and approximate quantification of small DNA fragments• Sharp bands• Supplied with loading dye for sample DNANote• Bands at 587 540 and 458 bp form an anomalous pattern on polyacrylamide gels • The shortest fragments oblique are not visible in standard electrophoresis • Fragment lengths are predicted by computer analysis of the respective DNA sequences
    https://www.bioz.com/result/iii 8 dna/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iii 8 dna - by Bioz Stars, 2021-04
    95/100 stars

    Images

    1) Product Images from "Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity"

    Article Title: Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity

    Journal:

    doi: 10.1007/s00439-008-0467-y

    Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual III-8. Solid-shaded large
    Figure Legend Snippet: Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual III-8. Solid-shaded large

    Techniques Used:

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Intercalative DNA binding governs fluorescence enhancement of SYBR Gold
    Article Snippet: We used the channel with absorption and emission wavelengths of 494 nm and 518 nm, respectively, which are the closest match to those of SYBR Gold (495 nm and 537 nm, respectively) and read out the fluorescence intensities at 24 °C from the top. .. For the gel electrophoresis we added Gel Loading Dye Purple (6x) (NEB) to pBR322 DNA. .. We used 1%-agarose (Carl Roth) gels and TAE buffer (40 mM Tris, 20 mM acetic acid, and 1 mM EDTA, pH 8.6).

    Hybridization:

    Article Title: Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity
    Article Snippet: .. Comparative genomic hybridization of I-8 and III-8 DNA on a 1 Mb resolution BAC array ( ) failed to identify any significant gains or losses. .. We also performed a genome-wide linkage analysis on the Brazilian family, which was genotyped using the same Affymetrix platform.

    BAC Assay:

    Article Title: Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity
    Article Snippet: .. Comparative genomic hybridization of I-8 and III-8 DNA on a 1 Mb resolution BAC array ( ) failed to identify any significant gains or losses. .. We also performed a genome-wide linkage analysis on the Brazilian family, which was genotyped using the same Affymetrix platform.

    Methylation:

    Article Title: Vascular histone deacetylation by pharmacological HDAC inhibition
    Article Snippet: The methylated fragments were then eluted in three distinct subpopulations using 0.6 M, 1 M, and 2 M salt elution buffers, respectively (supplied in MethylMiner kit). .. Methylated DNA fragments, eluted with 2 M salt buffer, were purified and selected for sequencing on the Illumina Genome Analyzer. .. Sequencing of the first 36 nt was performed on the Genome Analyzer IIx instrument (Illumina) according to the manufacturer’s protocols.

    Purification:

    Article Title: Vascular histone deacetylation by pharmacological HDAC inhibition
    Article Snippet: The methylated fragments were then eluted in three distinct subpopulations using 0.6 M, 1 M, and 2 M salt elution buffers, respectively (supplied in MethylMiner kit). .. Methylated DNA fragments, eluted with 2 M salt buffer, were purified and selected for sequencing on the Illumina Genome Analyzer. .. Sequencing of the first 36 nt was performed on the Genome Analyzer IIx instrument (Illumina) according to the manufacturer’s protocols.

    Sequencing:

    Article Title: Vascular histone deacetylation by pharmacological HDAC inhibition
    Article Snippet: The methylated fragments were then eluted in three distinct subpopulations using 0.6 M, 1 M, and 2 M salt elution buffers, respectively (supplied in MethylMiner kit). .. Methylated DNA fragments, eluted with 2 M salt buffer, were purified and selected for sequencing on the Illumina Genome Analyzer. .. Sequencing of the first 36 nt was performed on the Genome Analyzer IIx instrument (Illumina) according to the manufacturer’s protocols.

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  • 95
    Thermo Fisher iii 8 dna
    Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual <t>III-8.</t> Solid-shaded large
    Iii 8 Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iii 8 dna/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iii 8 dna - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    90
    Thermo Fisher gene exp polr3a hs01086939 m1
    Schematic representation of <t>RPC1</t> and 3D structure of amino acid exchanges. ( A ) Schematic representation of the RPC1 protein. Exonic and intronic mutations that result in missense, nonsense, or frameshift mutations are mapped to the respective domains. Intronic variants that affect splicing are labelled in blue. The amino acid conservation across species was determined for all missense variants from the UCSC Genome Browser (genome.ucsc.edu). ( B ) Protein 3D representations of <t>POLR3A</t> missense mutations p.C109S, p.L356P, p.L454F and p.A515V. 3D representations of POLR3A .
    Gene Exp Polr3a Hs01086939 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp polr3a hs01086939 m1/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp polr3a hs01086939 m1 - by Bioz Stars, 2021-04
    90/100 stars
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    94
    Thermo Fisher uracil dna glycosylase udg buffer
    Effects of differential folate repletion on inherent <t>DNA</t> damage and uracil misincorporation in 10 day folate depleted HaCaT cells as measured by comet assay with (solid bars) or without (open bars) <t>UDG</t> treatment. Cells cultured for 10 days in folate free
    Uracil Dna Glycosylase Udg Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uracil dna glycosylase udg buffer/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    uracil dna glycosylase udg buffer - by Bioz Stars, 2021-04
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    Image Search Results


    Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual III-8. Solid-shaded large

    Journal:

    Article Title: Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity

    doi: 10.1007/s00439-008-0467-y

    Figure Lengend Snippet: Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual III-8. Solid-shaded large

    Article Snippet: Comparative genomic hybridization of I-8 and III-8 DNA on a 1 Mb resolution BAC array ( ) failed to identify any significant gains or losses.

    Techniques:

    Schematic representation of RPC1 and 3D structure of amino acid exchanges. ( A ) Schematic representation of the RPC1 protein. Exonic and intronic mutations that result in missense, nonsense, or frameshift mutations are mapped to the respective domains. Intronic variants that affect splicing are labelled in blue. The amino acid conservation across species was determined for all missense variants from the UCSC Genome Browser (genome.ucsc.edu). ( B ) Protein 3D representations of POLR3A missense mutations p.C109S, p.L356P, p.L454F and p.A515V. 3D representations of POLR3A .

    Journal: Brain

    Article Title: Hypomorphic mutations in POLR3A are a frequent cause of sporadic and recessive spastic ataxia

    doi: 10.1093/brain/awx095

    Figure Lengend Snippet: Schematic representation of RPC1 and 3D structure of amino acid exchanges. ( A ) Schematic representation of the RPC1 protein. Exonic and intronic mutations that result in missense, nonsense, or frameshift mutations are mapped to the respective domains. Intronic variants that affect splicing are labelled in blue. The amino acid conservation across species was determined for all missense variants from the UCSC Genome Browser (genome.ucsc.edu). ( B ) Protein 3D representations of POLR3A missense mutations p.C109S, p.L356P, p.L454F and p.A515V. 3D representations of POLR3A .

    Article Snippet: For TaqMan® assays, the endogenous expression levels of POLR3A were quantified using the Hs01086939_m1 (exon boundery 8-9) TaqMan® Gene Expression Assay (Thermo Fisher Scientific).

    Techniques:

    Expression analysis of POLR3A. ( A ) Specific quantitative-PCR amplification of aberrant splice variant in fibroblast cells from two affected members of Family F1. Expression of POLR3A is normalized with YWHAZ and SDHA as reference genes. Expression levels are shown as relative fold expression levels of cells treated with cycloheximide (100 ng/ml) for 4 h compared to untreated cells. Inhibition of nonsense-mediated mRNA decay led to almost a 5-fold increase of the amount of aberrantly spliced transcript compared to untreated cells. ( B ) Figure demonstrating interindividual variability in POLR3A expression in controls (wild-type, WT), healthy heterozygous carriers of c.1909+22G > A, healthy heterozygous carriers of other truncating mutations, and affected individuals with compound heterozygous POLR3A mutations (i.e. c.1909+22G > A plus an additional truncating POLR3A mutation). Blue circles are Light Cycler (LC) data from Tübingen (normalized to controls that were available in Tübingen), red circles are TaqMan ® data from Bonn (normalized to controls available in Tübingen). Group-wise comparison showed the strongest reduction of total POLR3A levels in the group of affected individuals with compound heterozygous POLR3A mutations of ∼39% of that observed in wild-type. ( C ) Specific expression levels of the aberrant transcript of POLR3A in adult fibroblasts and leukocytes from Patients F1‐3 and F1‐7 shown relative to average expression level. ( D ) Specific expression levels of the aberrant transcript of POLR3A in different developmental stages. Fibroblasts from Patients F1‐3 and F1‐7 were reprogrammed to establish iPSC lines. Two iPSC clones from Patient F1‐3 (F1‐3C1 and F1‐3C2), were differentiated to neuroepithelial cells (neuroep. cells). The plot shows expression of cDNA template derived from iPSC lines and iPSC-derived neuroepithelial cells. The level of cryptic splice site activation is lowest in the iPSCs reprogrammed from patient fibroblasts.

    Journal: Brain

    Article Title: Hypomorphic mutations in POLR3A are a frequent cause of sporadic and recessive spastic ataxia

    doi: 10.1093/brain/awx095

    Figure Lengend Snippet: Expression analysis of POLR3A. ( A ) Specific quantitative-PCR amplification of aberrant splice variant in fibroblast cells from two affected members of Family F1. Expression of POLR3A is normalized with YWHAZ and SDHA as reference genes. Expression levels are shown as relative fold expression levels of cells treated with cycloheximide (100 ng/ml) for 4 h compared to untreated cells. Inhibition of nonsense-mediated mRNA decay led to almost a 5-fold increase of the amount of aberrantly spliced transcript compared to untreated cells. ( B ) Figure demonstrating interindividual variability in POLR3A expression in controls (wild-type, WT), healthy heterozygous carriers of c.1909+22G > A, healthy heterozygous carriers of other truncating mutations, and affected individuals with compound heterozygous POLR3A mutations (i.e. c.1909+22G > A plus an additional truncating POLR3A mutation). Blue circles are Light Cycler (LC) data from Tübingen (normalized to controls that were available in Tübingen), red circles are TaqMan ® data from Bonn (normalized to controls available in Tübingen). Group-wise comparison showed the strongest reduction of total POLR3A levels in the group of affected individuals with compound heterozygous POLR3A mutations of ∼39% of that observed in wild-type. ( C ) Specific expression levels of the aberrant transcript of POLR3A in adult fibroblasts and leukocytes from Patients F1‐3 and F1‐7 shown relative to average expression level. ( D ) Specific expression levels of the aberrant transcript of POLR3A in different developmental stages. Fibroblasts from Patients F1‐3 and F1‐7 were reprogrammed to establish iPSC lines. Two iPSC clones from Patient F1‐3 (F1‐3C1 and F1‐3C2), were differentiated to neuroepithelial cells (neuroep. cells). The plot shows expression of cDNA template derived from iPSC lines and iPSC-derived neuroepithelial cells. The level of cryptic splice site activation is lowest in the iPSCs reprogrammed from patient fibroblasts.

    Article Snippet: For TaqMan® assays, the endogenous expression levels of POLR3A were quantified using the Hs01086939_m1 (exon boundery 8-9) TaqMan® Gene Expression Assay (Thermo Fisher Scientific).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Amplification, Variant Assay, Inhibition, Mutagenesis, Clone Assay, Derivative Assay, Activation Assay

    Effects of differential folate repletion on inherent DNA damage and uracil misincorporation in 10 day folate depleted HaCaT cells as measured by comet assay with (solid bars) or without (open bars) UDG treatment. Cells cultured for 10 days in folate free

    Journal: Journal of photochemistry and photobiology. B, Biology

    Article Title: Photobiological Implications of Folate Depletion and Repletion in Cultured Human Keratinocytes

    doi: 10.1016/j.jphotobiol.2010.02.003

    Figure Lengend Snippet: Effects of differential folate repletion on inherent DNA damage and uracil misincorporation in 10 day folate depleted HaCaT cells as measured by comet assay with (solid bars) or without (open bars) UDG treatment. Cells cultured for 10 days in folate free

    Article Snippet: Briefly, after cell lysis, the slides were washed three times for 5 min each in uracil DNA glycosylase (UDG) buffer (60 mM Tris-HCl, 1 mM EDTA, 0.1 mg/mL BSA, pH 8.0) (Fermentas).

    Techniques: Single Cell Gel Electrophoresis, Cell Culture

    Effects of folate restriction on inherent DNA damage and uracil misincorporation as measured by comet assay with (solid bars) or without (open bars) uracil DNA glycosylase (UDG) treatment. Results are mean ± SEM. *** P

    Journal: Journal of photochemistry and photobiology. B, Biology

    Article Title: Photobiological Implications of Folate Depletion and Repletion in Cultured Human Keratinocytes

    doi: 10.1016/j.jphotobiol.2010.02.003

    Figure Lengend Snippet: Effects of folate restriction on inherent DNA damage and uracil misincorporation as measured by comet assay with (solid bars) or without (open bars) uracil DNA glycosylase (UDG) treatment. Results are mean ± SEM. *** P

    Article Snippet: Briefly, after cell lysis, the slides were washed three times for 5 min each in uracil DNA glycosylase (UDG) buffer (60 mM Tris-HCl, 1 mM EDTA, 0.1 mg/mL BSA, pH 8.0) (Fermentas).

    Techniques: Single Cell Gel Electrophoresis