rabbit specific ihc polymer detection kit hrp dab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit specific ihc polymer detection kit hrp dab
    Rabbit Specific Ihc Polymer Detection Kit Hrp Dab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit specific ihc polymer detection kit hrp dab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit specific ihc polymer detection kit hrp dab
    Rabbit Specific Ihc Polymer Detection Kit Hrp Dab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    signalstain boost ihc detection reagent kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc signalstain boost ihc detection reagent kit
    Signalstain Boost Ihc Detection Reagent Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti rabbit hrp dab detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti rabbit hrp dab detection kit
    Anti Rabbit Hrp Dab Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    signal stain ihc detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc signal stain ihc detection kit
    Antibodies used for tissue microarray Immunohistochemical analysis
    Signal Stain Ihc Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "PIK3CA alterations in Middle Eastern ovarian cancers"

    Article Title: PIK3CA alterations in Middle Eastern ovarian cancers

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-8-51

    Antibodies used for tissue microarray Immunohistochemical analysis
    Figure Legend Snippet: Antibodies used for tissue microarray Immunohistochemical analysis

    Techniques Used: Microarray, Immunohistochemical staining, Marker, Staining

    ihc detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ihc detection kit
    PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . <t>Immunohistochemical</t> <t>staining</t> was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.
    Ihc Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Attenuated Salmonella potentiate PD-L1 blockade immunotherapy in a preclinical model of colorectal cancer"

    Article Title: Attenuated Salmonella potentiate PD-L1 blockade immunotherapy in a preclinical model of colorectal cancer

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.1017780

    PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . Immunohistochemical staining was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.
    Figure Legend Snippet: PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . Immunohistochemical staining was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.

    Techniques Used: Expressing, In Vitro, Immunohistochemical staining, Staining

    Combination treatment increases MC38 tumor infiltration by CD4 + and CD8 + T cells and the expression of their effector molecules. MC38 tumor tissues were resected from non-treated, αPD-L1 and combination-treated mice on day 21 post implantation for further analyses using immunohistochemistry, flow cytometry and qRT-PCR. Representative immunohistochemical images for CD4 (A) and CD8 (C) in tumor tissues are presented for the control and combination-treated groups. Magnification 400×. Scale bar 20 μm. CD4 + (B) and CD8 + (D) cells were quantified in 15 HPF for control, αPD-L1 and combination-treated groups. Each data point represents the average of positive cells/HPF from a single mouse, pooled from 2 independent experiments. Representative flow cytometric dot plots and the combined result analyses for the percentages of CD4 + (E, F) and CD8 + (E, G) cells in MC38 tumors are illustrated. RNA was extracted from total MC38 tumor tissues and gene expression levels were determined using qRT-PCR. The effect of combination treatment on the expression levels of CXCL9 (H) , CXCL10 (I) , IFN-γ (J) and granzyme B (K) was assessed. Representative images for granzyme B staining in tumor tissues are presented for each group (L) . Graph depicts the number of cells/HPF (M) . Magnification 400×. Scale bar 20 μm. Each data point represents a single mouse pooled from two independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and **** (P ≤ 0.0001).
    Figure Legend Snippet: Combination treatment increases MC38 tumor infiltration by CD4 + and CD8 + T cells and the expression of their effector molecules. MC38 tumor tissues were resected from non-treated, αPD-L1 and combination-treated mice on day 21 post implantation for further analyses using immunohistochemistry, flow cytometry and qRT-PCR. Representative immunohistochemical images for CD4 (A) and CD8 (C) in tumor tissues are presented for the control and combination-treated groups. Magnification 400×. Scale bar 20 μm. CD4 + (B) and CD8 + (D) cells were quantified in 15 HPF for control, αPD-L1 and combination-treated groups. Each data point represents the average of positive cells/HPF from a single mouse, pooled from 2 independent experiments. Representative flow cytometric dot plots and the combined result analyses for the percentages of CD4 + (E, F) and CD8 + (E, G) cells in MC38 tumors are illustrated. RNA was extracted from total MC38 tumor tissues and gene expression levels were determined using qRT-PCR. The effect of combination treatment on the expression levels of CXCL9 (H) , CXCL10 (I) , IFN-γ (J) and granzyme B (K) was assessed. Representative images for granzyme B staining in tumor tissues are presented for each group (L) . Graph depicts the number of cells/HPF (M) . Magnification 400×. Scale bar 20 μm. Each data point represents a single mouse pooled from two independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and **** (P ≤ 0.0001).

    Techniques Used: Expressing, Immunohistochemistry, Flow Cytometry, Quantitative RT-PCR, Immunohistochemical staining, Staining

    Combination treatment of Salmonella and αPD-L1 induces apoptosis more efficiently than monotherapy. Representative immunohistochemical images for cleaved caspase-3 in tumor tissues are presented for control, Salmonella , αPD-L1 and combination-treated groups (A) . Magnification 400×. Scale bar 20 μm. Cleaved caspase 3 + cells were quantified in 15 HPF for the different groups (B) . Each data point represents the average of positive cells from a single mouse, pooled from 2 independent experiments for all groups except for the control group, from three independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and ns (no statistical significance, ≥ 0.05).
    Figure Legend Snippet: Combination treatment of Salmonella and αPD-L1 induces apoptosis more efficiently than monotherapy. Representative immunohistochemical images for cleaved caspase-3 in tumor tissues are presented for control, Salmonella , αPD-L1 and combination-treated groups (A) . Magnification 400×. Scale bar 20 μm. Cleaved caspase 3 + cells were quantified in 15 HPF for the different groups (B) . Each data point represents the average of positive cells from a single mouse, pooled from 2 independent experiments for all groups except for the control group, from three independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and ns (no statistical significance, ≥ 0.05).

    Techniques Used: Immunohistochemical staining

    signalstain cleaved caspase3 asp175 immunohistochemistry  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc signalstain cleaved caspase3 asp175 immunohistochemistry
    Signalstain Cleaved Caspase3 Asp175 Immunohistochemistry, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cleaved caspase3 ihc detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase3 ihc detection kit
    Cleaved Caspase3 Ihc Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    signalstain cleaved caspase 3 asp175 ihc detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc signalstain cleaved caspase 3 asp175 ihc detection kit
    Mice were inoculated subcutaneously with exponentially growing T24 and RT4 bladder cancer cells (5×10 6 cells). When tumor size reached 40 mm 3 in volume, mice were randomly divided into control and treated groups (n = 10). Intraperitoneal injections of ciglitazone were weekly administered at a dose of 15 mg/kg during three weeks. Control animals received only saline vehicle following an identical schedule. (A) The growth tumor curves were determined by measuring the tumor volume. * P <0.05, significant differences compared with untreated animals with the use of two-way ANOVA test (evaluation of the tumor volume development over time) ; ** P <0.05, significant differences between control and treated mice at each post-graft time with the use of two-tailed unpaired Student's t test. (B) Immunohistochemical staining of representative paraffin-embedded sections of tumors from untreated or ciglitazone-treated mice. Sections were fixed and stained for Ki-67 and active <t>caspase</t> <t>3.</t> Each panel is representative of 5 sections for each of ten tumors from control and ciglitazone-treated mice. Original magnification, ×20.
    Signalstain Cleaved Caspase 3 Asp175 Ihc Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The Antidiabetic Drug Ciglitazone Induces High Grade Bladder Cancer Cells Apoptosis through the Up-Regulation of TRAIL"

    Article Title: The Antidiabetic Drug Ciglitazone Induces High Grade Bladder Cancer Cells Apoptosis through the Up-Regulation of TRAIL

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0028354

    Mice were inoculated subcutaneously with exponentially growing T24 and RT4 bladder cancer cells (5×10 6 cells). When tumor size reached 40 mm 3 in volume, mice were randomly divided into control and treated groups (n = 10). Intraperitoneal injections of ciglitazone were weekly administered at a dose of 15 mg/kg during three weeks. Control animals received only saline vehicle following an identical schedule. (A) The growth tumor curves were determined by measuring the tumor volume. * P <0.05, significant differences compared with untreated animals with the use of two-way ANOVA test (evaluation of the tumor volume development over time) ; ** P <0.05, significant differences between control and treated mice at each post-graft time with the use of two-tailed unpaired Student's t test. (B) Immunohistochemical staining of representative paraffin-embedded sections of tumors from untreated or ciglitazone-treated mice. Sections were fixed and stained for Ki-67 and active caspase 3. Each panel is representative of 5 sections for each of ten tumors from control and ciglitazone-treated mice. Original magnification, ×20.
    Figure Legend Snippet: Mice were inoculated subcutaneously with exponentially growing T24 and RT4 bladder cancer cells (5×10 6 cells). When tumor size reached 40 mm 3 in volume, mice were randomly divided into control and treated groups (n = 10). Intraperitoneal injections of ciglitazone were weekly administered at a dose of 15 mg/kg during three weeks. Control animals received only saline vehicle following an identical schedule. (A) The growth tumor curves were determined by measuring the tumor volume. * P <0.05, significant differences compared with untreated animals with the use of two-way ANOVA test (evaluation of the tumor volume development over time) ; ** P <0.05, significant differences between control and treated mice at each post-graft time with the use of two-tailed unpaired Student's t test. (B) Immunohistochemical staining of representative paraffin-embedded sections of tumors from untreated or ciglitazone-treated mice. Sections were fixed and stained for Ki-67 and active caspase 3. Each panel is representative of 5 sections for each of ten tumors from control and ciglitazone-treated mice. Original magnification, ×20.

    Techniques Used: Two Tailed Test, Immunohistochemical staining, Staining

    signalstain cleaved caspase 3  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc signalstain cleaved caspase 3
    Tumor sections were stained using the DeadEnd colorimetric kit and cleaved <t>caspase-3</t> (Asp 175) IHC kit for TUNEL assay and cleaved caspase 3 expression as described in materials and methods respectively. The apoptotic tumor cells are stained brown. Percentages of TUNEL-positive and cleaved caspase 3-positive cells were quantitated by counting 100 cells from 6 random microscopic fields. Data are expressed as mean+SD (N = 6). One-way ANOVA followed by post Tukey test was used for statistical analysis to compare control and treated groups. P <0.01 (*, significantly different from untreated controls; ** , significantly different from Noscapine and Doxorubicin single treatments). Original magnification ×40 (Micron bar = 100 µm).
    Signalstain Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Antitumor Activity of Noscapine in Combination with Doxorubicin in Triple Negative Breast Cancer"

    Article Title: Antitumor Activity of Noscapine in Combination with Doxorubicin in Triple Negative Breast Cancer

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0017733

    Tumor sections were stained using the DeadEnd colorimetric kit and cleaved caspase-3 (Asp 175) IHC kit for TUNEL assay and cleaved caspase 3 expression as described in materials and methods respectively. The apoptotic tumor cells are stained brown. Percentages of TUNEL-positive and cleaved caspase 3-positive cells were quantitated by counting 100 cells from 6 random microscopic fields. Data are expressed as mean+SD (N = 6). One-way ANOVA followed by post Tukey test was used for statistical analysis to compare control and treated groups. P <0.01 (*, significantly different from untreated controls; ** , significantly different from Noscapine and Doxorubicin single treatments). Original magnification ×40 (Micron bar = 100 µm).
    Figure Legend Snippet: Tumor sections were stained using the DeadEnd colorimetric kit and cleaved caspase-3 (Asp 175) IHC kit for TUNEL assay and cleaved caspase 3 expression as described in materials and methods respectively. The apoptotic tumor cells are stained brown. Percentages of TUNEL-positive and cleaved caspase 3-positive cells were quantitated by counting 100 cells from 6 random microscopic fields. Data are expressed as mean+SD (N = 6). One-way ANOVA followed by post Tukey test was used for statistical analysis to compare control and treated groups. P <0.01 (*, significantly different from untreated controls; ** , significantly different from Noscapine and Doxorubicin single treatments). Original magnification ×40 (Micron bar = 100 µm).

    Techniques Used: Staining, TUNEL Assay, Expressing

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    Cell Signaling Technology Inc rabbit specific ihc polymer detection kit hrp dab
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    PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . <t>Immunohistochemical</t> <t>staining</t> was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.
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    PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . <t>Immunohistochemical</t> <t>staining</t> was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.
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    PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . <t>Immunohistochemical</t> <t>staining</t> was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.
    Cleaved Caspase3 Ihc Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mice were inoculated subcutaneously with exponentially growing T24 and RT4 bladder cancer cells (5×10 6 cells). When tumor size reached 40 mm 3 in volume, mice were randomly divided into control and treated groups (n = 10). Intraperitoneal injections of ciglitazone were weekly administered at a dose of 15 mg/kg during three weeks. Control animals received only saline vehicle following an identical schedule. (A) The growth tumor curves were determined by measuring the tumor volume. * P <0.05, significant differences compared with untreated animals with the use of two-way ANOVA test (evaluation of the tumor volume development over time) ; ** P <0.05, significant differences between control and treated mice at each post-graft time with the use of two-tailed unpaired Student's t test. (B) Immunohistochemical staining of representative paraffin-embedded sections of tumors from untreated or ciglitazone-treated mice. Sections were fixed and stained for Ki-67 and active <t>caspase</t> <t>3.</t> Each panel is representative of 5 sections for each of ten tumors from control and ciglitazone-treated mice. Original magnification, ×20.
    Signalstain Cleaved Caspase 3 Asp175 Ihc Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tumor sections were stained using the DeadEnd colorimetric kit and cleaved <t>caspase-3</t> (Asp 175) IHC kit for TUNEL assay and cleaved caspase 3 expression as described in materials and methods respectively. The apoptotic tumor cells are stained brown. Percentages of TUNEL-positive and cleaved caspase 3-positive cells were quantitated by counting 100 cells from 6 random microscopic fields. Data are expressed as mean+SD (N = 6). One-way ANOVA followed by post Tukey test was used for statistical analysis to compare control and treated groups. P <0.01 (*, significantly different from untreated controls; ** , significantly different from Noscapine and Doxorubicin single treatments). Original magnification ×40 (Micron bar = 100 µm).
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    Image Search Results


    Antibodies used for tissue microarray Immunohistochemical analysis

    Journal: Molecular Cancer

    Article Title: PIK3CA alterations in Middle Eastern ovarian cancers

    doi: 10.1186/1476-4598-8-51

    Figure Lengend Snippet: Antibodies used for tissue microarray Immunohistochemical analysis

    Article Snippet: p-AKT (Cytoplasmic & Nuclear) , 75/144 52.1 , Ser473 , Cell Signaling , Mouse Mono-clonal , Predilute , pH 9, microwave , Survival Marker; Signal Stain IHC detection Kit.

    Techniques: Microarray, Immunohistochemical staining, Marker, Staining

    PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . Immunohistochemical staining was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.

    Journal: Frontiers in Immunology

    Article Title: Attenuated Salmonella potentiate PD-L1 blockade immunotherapy in a preclinical model of colorectal cancer

    doi: 10.3389/fimmu.2022.1017780

    Figure Lengend Snippet: PD-1 and PD-L1 are expressed in MC38 tumors. Representative flow cytometric dot plots showing the expression of PD-L1 (A, C) and PD-1 (B, D) on MC38 tumor cells grown in vitro (A, B) or on single cell suspension of dissociated tumor tissues excised from mice 21 post subcutaneous implantation (C, D) . Immunohistochemical staining was also used to illustrate the expression of PD-L1 (E) and PD-1 (F) on MC38 tumor tissue sections. Magnification 400×. Scale bar 20 μm.

    Article Snippet: All immunohistochemical studies were done using the described protocol except for cleaved caspase-3 staining which was done following the protocol of IHC Detection Kit of the manufacturer (Cell Signaling Technology; #12692).

    Techniques: Expressing, In Vitro, Immunohistochemical staining, Staining

    Combination treatment increases MC38 tumor infiltration by CD4 + and CD8 + T cells and the expression of their effector molecules. MC38 tumor tissues were resected from non-treated, αPD-L1 and combination-treated mice on day 21 post implantation for further analyses using immunohistochemistry, flow cytometry and qRT-PCR. Representative immunohistochemical images for CD4 (A) and CD8 (C) in tumor tissues are presented for the control and combination-treated groups. Magnification 400×. Scale bar 20 μm. CD4 + (B) and CD8 + (D) cells were quantified in 15 HPF for control, αPD-L1 and combination-treated groups. Each data point represents the average of positive cells/HPF from a single mouse, pooled from 2 independent experiments. Representative flow cytometric dot plots and the combined result analyses for the percentages of CD4 + (E, F) and CD8 + (E, G) cells in MC38 tumors are illustrated. RNA was extracted from total MC38 tumor tissues and gene expression levels were determined using qRT-PCR. The effect of combination treatment on the expression levels of CXCL9 (H) , CXCL10 (I) , IFN-γ (J) and granzyme B (K) was assessed. Representative images for granzyme B staining in tumor tissues are presented for each group (L) . Graph depicts the number of cells/HPF (M) . Magnification 400×. Scale bar 20 μm. Each data point represents a single mouse pooled from two independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and **** (P ≤ 0.0001).

    Journal: Frontiers in Immunology

    Article Title: Attenuated Salmonella potentiate PD-L1 blockade immunotherapy in a preclinical model of colorectal cancer

    doi: 10.3389/fimmu.2022.1017780

    Figure Lengend Snippet: Combination treatment increases MC38 tumor infiltration by CD4 + and CD8 + T cells and the expression of their effector molecules. MC38 tumor tissues were resected from non-treated, αPD-L1 and combination-treated mice on day 21 post implantation for further analyses using immunohistochemistry, flow cytometry and qRT-PCR. Representative immunohistochemical images for CD4 (A) and CD8 (C) in tumor tissues are presented for the control and combination-treated groups. Magnification 400×. Scale bar 20 μm. CD4 + (B) and CD8 + (D) cells were quantified in 15 HPF for control, αPD-L1 and combination-treated groups. Each data point represents the average of positive cells/HPF from a single mouse, pooled from 2 independent experiments. Representative flow cytometric dot plots and the combined result analyses for the percentages of CD4 + (E, F) and CD8 + (E, G) cells in MC38 tumors are illustrated. RNA was extracted from total MC38 tumor tissues and gene expression levels were determined using qRT-PCR. The effect of combination treatment on the expression levels of CXCL9 (H) , CXCL10 (I) , IFN-γ (J) and granzyme B (K) was assessed. Representative images for granzyme B staining in tumor tissues are presented for each group (L) . Graph depicts the number of cells/HPF (M) . Magnification 400×. Scale bar 20 μm. Each data point represents a single mouse pooled from two independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and **** (P ≤ 0.0001).

    Article Snippet: All immunohistochemical studies were done using the described protocol except for cleaved caspase-3 staining which was done following the protocol of IHC Detection Kit of the manufacturer (Cell Signaling Technology; #12692).

    Techniques: Expressing, Immunohistochemistry, Flow Cytometry, Quantitative RT-PCR, Immunohistochemical staining, Staining

    Combination treatment of Salmonella and αPD-L1 induces apoptosis more efficiently than monotherapy. Representative immunohistochemical images for cleaved caspase-3 in tumor tissues are presented for control, Salmonella , αPD-L1 and combination-treated groups (A) . Magnification 400×. Scale bar 20 μm. Cleaved caspase 3 + cells were quantified in 15 HPF for the different groups (B) . Each data point represents the average of positive cells from a single mouse, pooled from 2 independent experiments for all groups except for the control group, from three independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and ns (no statistical significance, ≥ 0.05).

    Journal: Frontiers in Immunology

    Article Title: Attenuated Salmonella potentiate PD-L1 blockade immunotherapy in a preclinical model of colorectal cancer

    doi: 10.3389/fimmu.2022.1017780

    Figure Lengend Snippet: Combination treatment of Salmonella and αPD-L1 induces apoptosis more efficiently than monotherapy. Representative immunohistochemical images for cleaved caspase-3 in tumor tissues are presented for control, Salmonella , αPD-L1 and combination-treated groups (A) . Magnification 400×. Scale bar 20 μm. Cleaved caspase 3 + cells were quantified in 15 HPF for the different groups (B) . Each data point represents the average of positive cells from a single mouse, pooled from 2 independent experiments for all groups except for the control group, from three independent experiments. Asterisks denote statistically significant differences from control group, * (P ≤ 0.05), ** (P ≤ 0.01), *** (P ≤ 0.001) and ns (no statistical significance, ≥ 0.05).

    Article Snippet: All immunohistochemical studies were done using the described protocol except for cleaved caspase-3 staining which was done following the protocol of IHC Detection Kit of the manufacturer (Cell Signaling Technology; #12692).

    Techniques: Immunohistochemical staining

    Mice were inoculated subcutaneously with exponentially growing T24 and RT4 bladder cancer cells (5×10 6 cells). When tumor size reached 40 mm 3 in volume, mice were randomly divided into control and treated groups (n = 10). Intraperitoneal injections of ciglitazone were weekly administered at a dose of 15 mg/kg during three weeks. Control animals received only saline vehicle following an identical schedule. (A) The growth tumor curves were determined by measuring the tumor volume. * P <0.05, significant differences compared with untreated animals with the use of two-way ANOVA test (evaluation of the tumor volume development over time) ; ** P <0.05, significant differences between control and treated mice at each post-graft time with the use of two-tailed unpaired Student's t test. (B) Immunohistochemical staining of representative paraffin-embedded sections of tumors from untreated or ciglitazone-treated mice. Sections were fixed and stained for Ki-67 and active caspase 3. Each panel is representative of 5 sections for each of ten tumors from control and ciglitazone-treated mice. Original magnification, ×20.

    Journal: PLoS ONE

    Article Title: The Antidiabetic Drug Ciglitazone Induces High Grade Bladder Cancer Cells Apoptosis through the Up-Regulation of TRAIL

    doi: 10.1371/journal.pone.0028354

    Figure Lengend Snippet: Mice were inoculated subcutaneously with exponentially growing T24 and RT4 bladder cancer cells (5×10 6 cells). When tumor size reached 40 mm 3 in volume, mice were randomly divided into control and treated groups (n = 10). Intraperitoneal injections of ciglitazone were weekly administered at a dose of 15 mg/kg during three weeks. Control animals received only saline vehicle following an identical schedule. (A) The growth tumor curves were determined by measuring the tumor volume. * P <0.05, significant differences compared with untreated animals with the use of two-way ANOVA test (evaluation of the tumor volume development over time) ; ** P <0.05, significant differences between control and treated mice at each post-graft time with the use of two-tailed unpaired Student's t test. (B) Immunohistochemical staining of representative paraffin-embedded sections of tumors from untreated or ciglitazone-treated mice. Sections were fixed and stained for Ki-67 and active caspase 3. Each panel is representative of 5 sections for each of ten tumors from control and ciglitazone-treated mice. Original magnification, ×20.

    Article Snippet: Activated caspase 3 was detected with Apoptosis marker: SignalStain cleaved Caspase 3 (Asp175) IHC Detection kit (Cell Signaling).

    Techniques: Two Tailed Test, Immunohistochemical staining, Staining

    Tumor sections were stained using the DeadEnd colorimetric kit and cleaved caspase-3 (Asp 175) IHC kit for TUNEL assay and cleaved caspase 3 expression as described in materials and methods respectively. The apoptotic tumor cells are stained brown. Percentages of TUNEL-positive and cleaved caspase 3-positive cells were quantitated by counting 100 cells from 6 random microscopic fields. Data are expressed as mean+SD (N = 6). One-way ANOVA followed by post Tukey test was used for statistical analysis to compare control and treated groups. P <0.01 (*, significantly different from untreated controls; ** , significantly different from Noscapine and Doxorubicin single treatments). Original magnification ×40 (Micron bar = 100 µm).

    Journal: PLoS ONE

    Article Title: Antitumor Activity of Noscapine in Combination with Doxorubicin in Triple Negative Breast Cancer

    doi: 10.1371/journal.pone.0017733

    Figure Lengend Snippet: Tumor sections were stained using the DeadEnd colorimetric kit and cleaved caspase-3 (Asp 175) IHC kit for TUNEL assay and cleaved caspase 3 expression as described in materials and methods respectively. The apoptotic tumor cells are stained brown. Percentages of TUNEL-positive and cleaved caspase 3-positive cells were quantitated by counting 100 cells from 6 random microscopic fields. Data are expressed as mean+SD (N = 6). One-way ANOVA followed by post Tukey test was used for statistical analysis to compare control and treated groups. P <0.01 (*, significantly different from untreated controls; ** , significantly different from Noscapine and Doxorubicin single treatments). Original magnification ×40 (Micron bar = 100 µm).

    Article Snippet: Tumor tissue sections prepared from formalin-fixed, paraffin-embedded tumor tissues were used for IHC studies according to the protocol specified in the SignalStain™ Cleaved Caspase-3 (Asp 175) IHC kit (Cell Signaling, Beverly, MA).

    Techniques: Staining, TUNEL Assay, Expressing