igm antibody  (Thermo Fisher)


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    Name:
    IgM Antibody
    Description:
    Designed for immunofluorescence staining Thermo Scientific IgM FITC Labeled Antibody allows rapid and inexpensive assessment of tissues
    Catalog Number:
    rb-1922-r2
    Price:
    None
    Applications:
    Anatomical Pathology|Clinical
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher igm antibody
    Co-localization of PAB-reactive PLTA antigen, <t>IgA,</t> <t>IgM,</t> and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).
    Designed for immunofluorescence staining Thermo Scientific IgM FITC Labeled Antibody allows rapid and inexpensive assessment of tissues
    https://www.bioz.com/result/igm antibody/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    igm antibody - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis"

    Article Title: Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0192408

    Co-localization of PAB-reactive PLTA antigen, IgA, IgM, and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).
    Figure Legend Snippet: Co-localization of PAB-reactive PLTA antigen, IgA, IgM, and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).

    Techniques Used: Fluorescence, Immunohistochemistry

    Immuno-electron microscopy images of SRBs in sinus macrophages of sarcoid lymph nodes. A and B: IHC with PAB antibody after MT treatment, C: IHC with anti-IgA antibody, and D: IHC with anti-IgM antibody. Note that dense black-colored reaction products by each antibody were located along the peripheral rim of the SRBs. A similar distribution of PAB-reactivity was observed in a large spherical-shaped HW body (A).
    Figure Legend Snippet: Immuno-electron microscopy images of SRBs in sinus macrophages of sarcoid lymph nodes. A and B: IHC with PAB antibody after MT treatment, C: IHC with anti-IgA antibody, and D: IHC with anti-IgM antibody. Note that dense black-colored reaction products by each antibody were located along the peripheral rim of the SRBs. A similar distribution of PAB-reactivity was observed in a large spherical-shaped HW body (A).

    Techniques Used: Immuno-Electron Microscopy, Immunohistochemistry

    Insoluble immune complexes in sinus macrophages of control lymph nodes from colon cancer patients. In a representative case of control lymph nodes with IICs from colon cancer patients, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, many IgA-positive and a few IgM-positive small particles were detected (C and D, respectively), although a few PAB-reactive SRBs were detected with no difference in the number between the sections with and without MT treatment (F and E, respectively). Scale bar: 20 μm.
    Figure Legend Snippet: Insoluble immune complexes in sinus macrophages of control lymph nodes from colon cancer patients. In a representative case of control lymph nodes with IICs from colon cancer patients, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, many IgA-positive and a few IgM-positive small particles were detected (C and D, respectively), although a few PAB-reactive SRBs were detected with no difference in the number between the sections with and without MT treatment (F and E, respectively). Scale bar: 20 μm.

    Techniques Used: H&E Stain, Immunohistochemistry

    Insoluble immune complexes in sinus macrophages of control lymph nodes from patients with reactive lymphadenitis. In a representative case of control lymph nodes with IICs from patients with reactive lymphadenitis, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, IgA- and IgM-positive SRBs were detected (C and D, respectively), and PAB-reactive SRBs were also detected with a small increase in the number after MT treatment (F). Scale bar: 20 μm.
    Figure Legend Snippet: Insoluble immune complexes in sinus macrophages of control lymph nodes from patients with reactive lymphadenitis. In a representative case of control lymph nodes with IICs from patients with reactive lymphadenitis, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, IgA- and IgM-positive SRBs were detected (C and D, respectively), and PAB-reactive SRBs were also detected with a small increase in the number after MT treatment (F). Scale bar: 20 μm.

    Techniques Used: H&E Stain, Immunohistochemistry

    P . acnes -derived insoluble immune complexes in sinus macrophages of sarcoid lymph nodes. In a representative case of sarcoid lymph nodes, identical areas of the lesion including a lymphatic sinus and adjacent paracortical area with a sarcoid granuloma are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, the distributions of IgA- and IgM-positive SRBs (C and D) and PAB-reactive SRBs (F) were similar. Note the few PAB-reactive SRBs with weak intensity (indicated by the arrow) in the granuloma after MT treatment (F). Scale bar: 20 μm.
    Figure Legend Snippet: P . acnes -derived insoluble immune complexes in sinus macrophages of sarcoid lymph nodes. In a representative case of sarcoid lymph nodes, identical areas of the lesion including a lymphatic sinus and adjacent paracortical area with a sarcoid granuloma are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, the distributions of IgA- and IgM-positive SRBs (C and D) and PAB-reactive SRBs (F) were similar. Note the few PAB-reactive SRBs with weak intensity (indicated by the arrow) in the granuloma after MT treatment (F). Scale bar: 20 μm.

    Techniques Used: Derivative Assay, H&E Stain, Immunohistochemistry

    2) Product Images from "MS2 VLP-based delivery of microRNA-146a inhibits autoantibody production in lupus-prone mice"

    Article Title: MS2 VLP-based delivery of microRNA-146a inhibits autoantibody production in lupus-prone mice

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S37990

    Decreased autoantibody production after MS2-miR146a VLP administration. ( A ) Anti-dsDNA and ANA levels in MS2-miR146a VLP-treated BXSB mice and their respective control mice (approximately 18 weeks of age, n = 5 per group) were evaluated by ELISA. ( B and C ) Immunofluorescence assay was used to verify the reduced expression of ANA in samples from MS2-miR146a VLP-treated BXSB mice (diluted 200-fold). Representative photomicrographs of ANA fluorescence in treated and respective control groups are shown. Fluorescence patterns were detected by fluorescence microscopy at 400× magnification. The fluorescence intensity of ANA in samples from placebo-treated BXSB mice was designated as 100%. ( D ) Total IgG and total IgM concentrations in MS2-miR146a VLP-treated BXSB mice and their respective control mice (approximately 18 weeks of age, n = 5 per group) were analyzed by ELISA. Notes: * P
    Figure Legend Snippet: Decreased autoantibody production after MS2-miR146a VLP administration. ( A ) Anti-dsDNA and ANA levels in MS2-miR146a VLP-treated BXSB mice and their respective control mice (approximately 18 weeks of age, n = 5 per group) were evaluated by ELISA. ( B and C ) Immunofluorescence assay was used to verify the reduced expression of ANA in samples from MS2-miR146a VLP-treated BXSB mice (diluted 200-fold). Representative photomicrographs of ANA fluorescence in treated and respective control groups are shown. Fluorescence patterns were detected by fluorescence microscopy at 400× magnification. The fluorescence intensity of ANA in samples from placebo-treated BXSB mice was designated as 100%. ( D ) Total IgG and total IgM concentrations in MS2-miR146a VLP-treated BXSB mice and their respective control mice (approximately 18 weeks of age, n = 5 per group) were analyzed by ELISA. Notes: * P

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Expressing, Fluorescence, Microscopy

    3) Product Images from "Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis"

    Article Title: Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0192408

    Insoluble immune complexes in sinus macrophages of control lymph nodes from colon cancer patients. In a representative case of control lymph nodes with IICs from colon cancer patients, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, many IgA-positive and a few IgM-positive small particles were detected (C and D, respectively), although a few PAB-reactive SRBs were detected with no difference in the number between the sections with and without MT treatment (F and E, respectively). Scale bar: 20 μm.
    Figure Legend Snippet: Insoluble immune complexes in sinus macrophages of control lymph nodes from colon cancer patients. In a representative case of control lymph nodes with IICs from colon cancer patients, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, many IgA-positive and a few IgM-positive small particles were detected (C and D, respectively), although a few PAB-reactive SRBs were detected with no difference in the number between the sections with and without MT treatment (F and E, respectively). Scale bar: 20 μm.

    Techniques Used: H&E Stain, Immunohistochemistry

    Co-localization of PAB-reactive PLTA antigen, IgA, IgM, and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).
    Figure Legend Snippet: Co-localization of PAB-reactive PLTA antigen, IgA, IgM, and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).

    Techniques Used: Fluorescence, Immunohistochemistry

    Immuno-electron microscopy images of SRBs in sinus macrophages of sarcoid lymph nodes. A and B: IHC with PAB antibody after MT treatment, C: IHC with anti-IgA antibody, and D: IHC with anti-IgM antibody. Note that dense black-colored reaction products by each antibody were located along the peripheral rim of the SRBs. A similar distribution of PAB-reactivity was observed in a large spherical-shaped HW body (A).
    Figure Legend Snippet: Immuno-electron microscopy images of SRBs in sinus macrophages of sarcoid lymph nodes. A and B: IHC with PAB antibody after MT treatment, C: IHC with anti-IgA antibody, and D: IHC with anti-IgM antibody. Note that dense black-colored reaction products by each antibody were located along the peripheral rim of the SRBs. A similar distribution of PAB-reactivity was observed in a large spherical-shaped HW body (A).

    Techniques Used: Immuno-Electron Microscopy, Immunohistochemistry

    Insoluble immune complexes in sinus macrophages of control lymph nodes from patients with reactive lymphadenitis. In a representative case of control lymph nodes with IICs from patients with reactive lymphadenitis, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, IgA- and IgM-positive SRBs were detected (C and D, respectively), and PAB-reactive SRBs were also detected with a small increase in the number after MT treatment (F). Scale bar: 20 μm.
    Figure Legend Snippet: Insoluble immune complexes in sinus macrophages of control lymph nodes from patients with reactive lymphadenitis. In a representative case of control lymph nodes with IICs from patients with reactive lymphadenitis, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, IgA- and IgM-positive SRBs were detected (C and D, respectively), and PAB-reactive SRBs were also detected with a small increase in the number after MT treatment (F). Scale bar: 20 μm.

    Techniques Used: H&E Stain, Immunohistochemistry

    Higher magnification of small round bodies detected in the lymphatic sinus of sarcoid lymph nodes by IHC with each antibody. A: IHC with PAB antibody after MT treatment, B: IHC with anti-IgG antibody, C: IHC with anti-IgA antibody, D: IHC with anti-IgM antibody, E: IHC with anti-C1q antibody, and F: IHC with anti-C3c antibody. Note that the dark brown-colored reaction products produced by each antibody are all located along the peripheral rim of the small round bodies. Scale bar: 5 μm.
    Figure Legend Snippet: Higher magnification of small round bodies detected in the lymphatic sinus of sarcoid lymph nodes by IHC with each antibody. A: IHC with PAB antibody after MT treatment, B: IHC with anti-IgG antibody, C: IHC with anti-IgA antibody, D: IHC with anti-IgM antibody, E: IHC with anti-C1q antibody, and F: IHC with anti-C3c antibody. Note that the dark brown-colored reaction products produced by each antibody are all located along the peripheral rim of the small round bodies. Scale bar: 5 μm.

    Techniques Used: Immunohistochemistry, Produced

    P . acnes -derived insoluble immune complexes in sinus macrophages of sarcoid lymph nodes. In a representative case of sarcoid lymph nodes, identical areas of the lesion including a lymphatic sinus and adjacent paracortical area with a sarcoid granuloma are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, the distributions of IgA- and IgM-positive SRBs (C and D) and PAB-reactive SRBs (F) were similar. Note the few PAB-reactive SRBs with weak intensity (indicated by the arrow) in the granuloma after MT treatment (F). Scale bar: 20 μm.
    Figure Legend Snippet: P . acnes -derived insoluble immune complexes in sinus macrophages of sarcoid lymph nodes. In a representative case of sarcoid lymph nodes, identical areas of the lesion including a lymphatic sinus and adjacent paracortical area with a sarcoid granuloma are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, the distributions of IgA- and IgM-positive SRBs (C and D) and PAB-reactive SRBs (F) were similar. Note the few PAB-reactive SRBs with weak intensity (indicated by the arrow) in the granuloma after MT treatment (F). Scale bar: 20 μm.

    Techniques Used: Derivative Assay, H&E Stain, Immunohistochemistry

    4) Product Images from "Effects of experimental cervical spinal cord injury on peripheral adaptive immunity"

    Article Title: Effects of experimental cervical spinal cord injury on peripheral adaptive immunity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0241285

    Changes in serum IgG and IgM immunoglobulin levels following cervical SCI. (a, b) Serum IgG and IgM levels declined significantly at 2 weeks post-cervical SCI compared to shams. In rats with cervical SCI, IgG immunoglobulin normalized to sham-levels at later time points. IgM levels were statistically higher in the SCI groups compared to shams at 10 weeks of injury. Naïve rats had significantly higher levels of IgG and IgM antibodies compared to sham rats at 10 weeks. One-way ANOVA with Holm-Sidak’s post-hoc test was performed for IgG data (2 weeks), and Kruskal Wallis analysis with Dunn’s multiple comparisons post-hoc tests were performed for all other data. * Indicates significant difference between sham and SCI groups, whereas # indicates significant difference between sham and naïve groups. */ # p
    Figure Legend Snippet: Changes in serum IgG and IgM immunoglobulin levels following cervical SCI. (a, b) Serum IgG and IgM levels declined significantly at 2 weeks post-cervical SCI compared to shams. In rats with cervical SCI, IgG immunoglobulin normalized to sham-levels at later time points. IgM levels were statistically higher in the SCI groups compared to shams at 10 weeks of injury. Naïve rats had significantly higher levels of IgG and IgM antibodies compared to sham rats at 10 weeks. One-way ANOVA with Holm-Sidak’s post-hoc test was performed for IgG data (2 weeks), and Kruskal Wallis analysis with Dunn’s multiple comparisons post-hoc tests were performed for all other data. * Indicates significant difference between sham and SCI groups, whereas # indicates significant difference between sham and naïve groups. */ # p

    Techniques Used:

    5) Product Images from "A cytoplasmic protein, bystin, interacts with trophinin, tastin, and cytokeratin and may be involved in trophinin-mediated cell adhesion between trophoblast and endometrial epithelial cells"

    Article Title: A cytoplasmic protein, bystin, interacts with trophinin, tastin, and cytokeratin and may be involved in trophinin-mediated cell adhesion between trophoblast and endometrial epithelial cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    Immunofluorescence micrographs of HT-H cells stained with antibodies for trophinin, bystin, tastin, and cytokeratin. ( A and B ) Double immunostaining showing trophinin ( A ) and bystin ( B ). Unpermeabilized cells were stained with fluorescein isothiocyanate (FITC)-conjugated anti-trophinin antibody ( A ), permeabilized, and stained with rhodamine-conjugated anti-bystin antibody ( B ). These photographs focused on the upper cell surface where trophinin is present. Accordingly, only a fraction of bystin localized near upper plasma membranes is shown. ( C and D ) Double immunostaining showing bystin ( C ) and tastin ( D ). Cells were permeabilized and stained with unconjugated anti-bystin and anti-tastin antibodies, followed by FITC-conjugated goat anti-mouse IgM antibody for bystin ( C ) and rhodamine-conjugated goat anti-mouse IgG antibody for tastin ( D ). ( E and F ) Double immunostaining showing bystin ( E ) and cytokeratin 8 ( F ). Cells were permeabilized and stained with unconjugated anti-bystin and anti-cytokeratin antibodies, followed by FITC-conjugated goat anti-mouse IgM antibody for bystin ( E ) and rhodamine conjugated goat anti-rat IgG antibody for cytokeratin 8 ( F ). All photographs are presented at the same magnification. (Bar = 20 μm.)
    Figure Legend Snippet: Immunofluorescence micrographs of HT-H cells stained with antibodies for trophinin, bystin, tastin, and cytokeratin. ( A and B ) Double immunostaining showing trophinin ( A ) and bystin ( B ). Unpermeabilized cells were stained with fluorescein isothiocyanate (FITC)-conjugated anti-trophinin antibody ( A ), permeabilized, and stained with rhodamine-conjugated anti-bystin antibody ( B ). These photographs focused on the upper cell surface where trophinin is present. Accordingly, only a fraction of bystin localized near upper plasma membranes is shown. ( C and D ) Double immunostaining showing bystin ( C ) and tastin ( D ). Cells were permeabilized and stained with unconjugated anti-bystin and anti-tastin antibodies, followed by FITC-conjugated goat anti-mouse IgM antibody for bystin ( C ) and rhodamine-conjugated goat anti-mouse IgG antibody for tastin ( D ). ( E and F ) Double immunostaining showing bystin ( E ) and cytokeratin 8 ( F ). Cells were permeabilized and stained with unconjugated anti-bystin and anti-cytokeratin antibodies, followed by FITC-conjugated goat anti-mouse IgM antibody for bystin ( E ) and rhodamine conjugated goat anti-rat IgG antibody for cytokeratin 8 ( F ). All photographs are presented at the same magnification. (Bar = 20 μm.)

    Techniques Used: Immunofluorescence, Staining, Double Immunostaining

    6) Product Images from "Production of IgG antibodies to pneumococcal polysaccharides is associated with expansion of ICOS+ circulating memory T follicular-helper cells which is impaired by HIV infection"

    Article Title: Production of IgG antibodies to pneumococcal polysaccharides is associated with expansion of ICOS+ circulating memory T follicular-helper cells which is impaired by HIV infection

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0176641

    PcP specific IgG + and IgM + memory B cells in HIV patients and HIV seronegative subjects pre-vaccination with PcPs. PBMCs were stimulated in vitro for 5 days with a combination of IL-2 and R848. ( A ) IgM + memory B cells to PcP 4, 6B, 9V and 14 ( B ) IgG + memory B cells to PcP 4, 6B, 9V and 14. Representative ELISpot images for PcP serotype 4-specific ( C ) IgM + memory B cells and ( D ) IgG + memory B cells. Data are presented as memory B cells /5.0x10 4 PBMC with background values subtracted. The horizontal lines indicate median values. Differences between groups were tested using Mann-Whitney tests. n.s., not significant and p
    Figure Legend Snippet: PcP specific IgG + and IgM + memory B cells in HIV patients and HIV seronegative subjects pre-vaccination with PcPs. PBMCs were stimulated in vitro for 5 days with a combination of IL-2 and R848. ( A ) IgM + memory B cells to PcP 4, 6B, 9V and 14 ( B ) IgG + memory B cells to PcP 4, 6B, 9V and 14. Representative ELISpot images for PcP serotype 4-specific ( C ) IgM + memory B cells and ( D ) IgG + memory B cells. Data are presented as memory B cells /5.0x10 4 PBMC with background values subtracted. The horizontal lines indicate median values. Differences between groups were tested using Mann-Whitney tests. n.s., not significant and p

    Techniques Used: In Vitro, Enzyme-linked Immunospot, MANN-WHITNEY

    7) Product Images from "Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules"

    Article Title: Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules

    Journal: Veterinary Immunology and Immunopathology

    doi: 10.1016/j.vetimm.2016.12.003

    Hamster blood leukocytes. ( Fig. 1 A) The major populations of cells were visualized by side vs forward light scatter, dot plot and colour coded for cell subsets: orange = lymphocytes (L), blue = monocytes (M), red = granulocytes (G). It should be noted that gating for monocytes may include large lymphocytes. There is no distinct border separating lymphocytes from monocytes. ( Fig. 1 B) Example of cells incubated with a mixture of anti-IgG1, IgG2a, and IgG2b 2nd step reagents alone to show there was no background attributed to nonspecific labelling and the relative position of colour coded granulocytes, monocytes, and lymphocytes visualized in side scatter vs fluorescence. ( Fig. 1 C) Typical pattern of labelling with mAbs specific for MHC II cross species for humans, cattle, goats, sheep, and llama/alpaca. ( Fig. 1 D) Typical pattern of labelling with mAbs specific for CD18 cross species for human, cattle, goats, sheep, llama/alpaca, horse, dogs, and cats. ( Fig. 1 E) Unique pattern of expression of a mAb-defined molecule on all lymphocytes and apparent expression on a subset of monocytes. ( Fig. 1 F) Expression of a mAb-defined molecule on granulocytes (Background labelling of lymphocytes is attributable to cross reactive anti-IgM antibody present in the 2nd step reagent used in these studies). ( Fig. 1 G) Typical pattern of labelling with mAbs specific for CD45 cross species in humans, cattle, goats, sheep, llama/alpaca, rabbit. ( Fig. 1 H) Pattern of labelling similar to CD11a cross species in humans, cattle, goats, sheep, rabbit. ( Fig. 1 I) Pattern of labelling similar to CD44 cross species in humans, cattle, goats, sheep, horse, rabbits. (Fig, 1J) Pattern of labelling with no apparent match to known LDMs. It should be noted that multiple hamsters were used at WSU during the past 28 years to develop and characterize the mAbs described in this report. On some occasions, only one hamster was used to obtain some of the information presented here and on other occasions, multiple hamsters were used to pool blood for analysis. The best representative flow cytometric profiles were selected from different data sets for presentation here. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Hamster blood leukocytes. ( Fig. 1 A) The major populations of cells were visualized by side vs forward light scatter, dot plot and colour coded for cell subsets: orange = lymphocytes (L), blue = monocytes (M), red = granulocytes (G). It should be noted that gating for monocytes may include large lymphocytes. There is no distinct border separating lymphocytes from monocytes. ( Fig. 1 B) Example of cells incubated with a mixture of anti-IgG1, IgG2a, and IgG2b 2nd step reagents alone to show there was no background attributed to nonspecific labelling and the relative position of colour coded granulocytes, monocytes, and lymphocytes visualized in side scatter vs fluorescence. ( Fig. 1 C) Typical pattern of labelling with mAbs specific for MHC II cross species for humans, cattle, goats, sheep, and llama/alpaca. ( Fig. 1 D) Typical pattern of labelling with mAbs specific for CD18 cross species for human, cattle, goats, sheep, llama/alpaca, horse, dogs, and cats. ( Fig. 1 E) Unique pattern of expression of a mAb-defined molecule on all lymphocytes and apparent expression on a subset of monocytes. ( Fig. 1 F) Expression of a mAb-defined molecule on granulocytes (Background labelling of lymphocytes is attributable to cross reactive anti-IgM antibody present in the 2nd step reagent used in these studies). ( Fig. 1 G) Typical pattern of labelling with mAbs specific for CD45 cross species in humans, cattle, goats, sheep, llama/alpaca, rabbit. ( Fig. 1 H) Pattern of labelling similar to CD11a cross species in humans, cattle, goats, sheep, rabbit. ( Fig. 1 I) Pattern of labelling similar to CD44 cross species in humans, cattle, goats, sheep, horse, rabbits. (Fig, 1J) Pattern of labelling with no apparent match to known LDMs. It should be noted that multiple hamsters were used at WSU during the past 28 years to develop and characterize the mAbs described in this report. On some occasions, only one hamster was used to obtain some of the information presented here and on other occasions, multiple hamsters were used to pool blood for analysis. The best representative flow cytometric profiles were selected from different data sets for presentation here. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Incubation, Fluorescence, Expressing, Flow Cytometry

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    Staining:

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    FACS:

    Article Title: Immune Tolerance to Apoptotic Self Is Mediated Primarily by Regulatory B1a Cells
    Article Snippet: .. Splenic CD19+ve B cells were FACs sorted into IL-10-GFP+ve or −ve fractions, phenotyped and cultured for 10 days in the presence of MegaAPRIL [Adipogen (200 ng/ml)], CpGB [ODN1826 Eurofins MWG Operon (1 μg/ml)], and IL-4 [R & D System (50 ng/ml)], after which culture supernatants were checked for IgM and IgG levels (Ready-SET-Go ELISA kit eBioScience). .. Highly purified IL-10-GFP+ve B1a cells, generated in vivo , were fused with SP2/0 cells to generate hybridomas.

    Cell Culture:

    Article Title: Immune Tolerance to Apoptotic Self Is Mediated Primarily by Regulatory B1a Cells
    Article Snippet: .. Splenic CD19+ve B cells were FACs sorted into IL-10-GFP+ve or −ve fractions, phenotyped and cultured for 10 days in the presence of MegaAPRIL [Adipogen (200 ng/ml)], CpGB [ODN1826 Eurofins MWG Operon (1 μg/ml)], and IL-4 [R & D System (50 ng/ml)], after which culture supernatants were checked for IgM and IgG levels (Ready-SET-Go ELISA kit eBioScience). .. Highly purified IL-10-GFP+ve B1a cells, generated in vivo , were fused with SP2/0 cells to generate hybridomas.

    Magnetic Beads:

    Article Title: Multiple Aspects of PIP2 Involvement in C. elegans Gametogenesis
    Article Snippet: .. For the precipitation, anti-PIP2 (2 µg), control anti-mouse IgM antibody (2 µg), and protein L magnetic beads (50 µL of slurry) were used according to the manufacturer’s protocol (Pierce, Thermo Scientific, Waltham, MA, USA). .. For the dot blot, the N2 worms were subjected to the ppk-1 ( RNAi ) or the control RNAi for 48 h. After the RNAi treatment, the single worms were lysed in a lysis buffer (50 mM KCl; 10 mM Tris (pH 8.3); 2.5 mM MgCl2 ; 0.45% NP-40; 0.45% Tween-20) in a total volume of 10 µL.

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    Thermo Fisher mouse anti human igm secondary antibody
    Profiling seroconversion in COVID-19. Simoa serological assay results for <t>IgG,</t> <t>IgM</t> and IgA against the four viral targets (spike, S1 subunit, RBD and nucleocapsid) for pre-pandemic samples (light blue; n = 200), nasopharyngeal (NP) PCR-negative samples (dark blue; n = 100) and SARS-CoV-2-positive samples (black (immunocompetent; n = 141) or red (immunosuppressed; n = 31)). The SARS-CoV-2-positive samples were divided into four groups according to time since since the first positive nasopharyngeal RT–PCR test. Black lines indicate the median normalized AEB value of each population.
    Mouse Anti Human Igm Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human igm secondary antibody/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
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    mouse anti human igm secondary antibody - by Bioz Stars, 2021-01
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    90
    Thermo Fisher horseradish peroxidase conjugated goat anti mouse igm
    MHC-I and MHC-II are involved in antibody isotype switching. B2m KO, MHC-II KO, and WT C57BL/6 mice were vaccinated s.c. with 10 μg of PIV and challenged i.p. with 1 × 10 7 genomic copies of C. burnetii NMI 28 dpv. Mice receiving Alhydrogel adjuvant alone served as unvaccinated controls. C. burnetii NMI-specific serum <t>IgM</t> (A to C) and <t>IgG</t> (D to F) were evaluated weekly following vaccination. Specific IgM (G) and IgG (H) were also evaluated 14 dpi. Each experimental group includes five mice, with error bars representing the standard deviations from the mean. **, P
    Horseradish Peroxidase Conjugated Goat Anti Mouse Igm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated goat anti mouse igm/product/Thermo Fisher
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    horseradish peroxidase conjugated goat anti mouse igm - by Bioz Stars, 2021-01
    90/100 stars
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    88
    Thermo Fisher goat anti mouse igm secondary antibody
    The immunogenicity of M2FA-lysine-BSA in the absence of adjuvant and anti-M2FA antibody titers in intact mice. C57BL/6 mice were injected i.p. with M2FA-lysine-BSA or BSA in the absence of adjuvant. The antibody titers of <t>IgG</t> ( a ) and <t>IgM</t> ( b ) against M2FA-lysine were detected using MFA-6ACA-KLH-coated plates. The anti-M2AA antibody titers were clearly increased in M2FA-lysine-BSA-immunized mice compared to the controls (BSA-treated mice). Values are mean and SD. ( c ) Intact female C57BL/6 mice (n = 4 or 5 per group) with different ages showed significantly different anti-M2FA IgG titers. Values are mean and SD. (*p
    Goat Anti Mouse Igm Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igm secondary antibody/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igm secondary antibody - by Bioz Stars, 2021-01
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    Image Search Results


    Profiling seroconversion in COVID-19. Simoa serological assay results for IgG, IgM and IgA against the four viral targets (spike, S1 subunit, RBD and nucleocapsid) for pre-pandemic samples (light blue; n = 200), nasopharyngeal (NP) PCR-negative samples (dark blue; n = 100) and SARS-CoV-2-positive samples (black (immunocompetent; n = 141) or red (immunosuppressed; n = 31)). The SARS-CoV-2-positive samples were divided into four groups according to time since since the first positive nasopharyngeal RT–PCR test. Black lines indicate the median normalized AEB value of each population.

    Journal: Nature Biomedical Engineering

    Article Title: Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19

    doi: 10.1038/s41551-020-00611-x

    Figure Lengend Snippet: Profiling seroconversion in COVID-19. Simoa serological assay results for IgG, IgM and IgA against the four viral targets (spike, S1 subunit, RBD and nucleocapsid) for pre-pandemic samples (light blue; n = 200), nasopharyngeal (NP) PCR-negative samples (dark blue; n = 100) and SARS-CoV-2-positive samples (black (immunocompetent; n = 141) or red (immunosuppressed; n = 31)). The SARS-CoV-2-positive samples were divided into four groups according to time since since the first positive nasopharyngeal RT–PCR test. Black lines indicate the median normalized AEB value of each population.

    Article Snippet: Anti-human immunoglobulin antibodies were diluted in Homebrew Detector/Sample Diluent to final concentrations of 7.73 ng ml−1 IgG (Bethyl Labratories; A80-148B), 216 ng ml−1 IgM (Thermo Fisher Scientific; MII0401) and 150 ng ml−1 IgA (Abcam; ab214003).

    Techniques: Serologic Assay, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    Classification of COVID-19 using Simoa serological assays. a , Left: ROC curves for all positive cases ( n = 141) in the pre-pandemic cohort ( n = 199) using either the full marker panel model (green), the early-stage model (blue) or the late-stage model (red). Middle: ROC curves for late-stage cases (more than 1 week after positive nasopharyngeal PCR test; n = 50) in the pre-pandemic cohort ( n = 199) using either the full marker panel model (green) or the late-stage model (red). Right: ROC curves for early-stage cases (first week after positive nasopharyngeal PCR test; n = 91) in the pre-pandemic cohort ( n = 199) using either the full marker panel model (green) or the early-stage model (blue). AUC values and 95% confidence intervals (CIs) are shown for each graph. b , Simoa serological assay results for IgG, IgM and IgA against the four viral targets (spike, S1 subunit, RBD and nucleocapsid) for pre-pandemic samples (light blue; n = 232) and nasopharyngeal RT–PCR-positive samples (grey; n = 68). Statistical significance was determined using Mann–Whitney U -tests. All U -tests were two tailed and did not correct for multiple comparisons. Black lines indicate the median normalized AEB value of each population.

    Journal: Nature Biomedical Engineering

    Article Title: Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19

    doi: 10.1038/s41551-020-00611-x

    Figure Lengend Snippet: Classification of COVID-19 using Simoa serological assays. a , Left: ROC curves for all positive cases ( n = 141) in the pre-pandemic cohort ( n = 199) using either the full marker panel model (green), the early-stage model (blue) or the late-stage model (red). Middle: ROC curves for late-stage cases (more than 1 week after positive nasopharyngeal PCR test; n = 50) in the pre-pandemic cohort ( n = 199) using either the full marker panel model (green) or the late-stage model (red). Right: ROC curves for early-stage cases (first week after positive nasopharyngeal PCR test; n = 91) in the pre-pandemic cohort ( n = 199) using either the full marker panel model (green) or the early-stage model (blue). AUC values and 95% confidence intervals (CIs) are shown for each graph. b , Simoa serological assay results for IgG, IgM and IgA against the four viral targets (spike, S1 subunit, RBD and nucleocapsid) for pre-pandemic samples (light blue; n = 232) and nasopharyngeal RT–PCR-positive samples (grey; n = 68). Statistical significance was determined using Mann–Whitney U -tests. All U -tests were two tailed and did not correct for multiple comparisons. Black lines indicate the median normalized AEB value of each population.

    Article Snippet: Anti-human immunoglobulin antibodies were diluted in Homebrew Detector/Sample Diluent to final concentrations of 7.73 ng ml−1 IgG (Bethyl Labratories; A80-148B), 216 ng ml−1 IgM (Thermo Fisher Scientific; MII0401) and 150 ng ml−1 IgA (Abcam; ab214003).

    Techniques: Marker, Polymerase Chain Reaction, Serologic Assay, Reverse Transcription Polymerase Chain Reaction, MANN-WHITNEY, Two Tailed Test

    Schematic of the Simoa serological assay. Plasma is incubated with four types of dye-encoded beads that are each coupled to one of four viral targets (spike, S1, RBD and nucleocapsid). IgG, IgA and IgM antibodies specific to the SARS-CoV-2 targets bind to the viral antigen-coated beads. After washing, beads are introduced to biotinylated anti-human immunoglobulin antibodies to label either IgG, IgM or IgA in the different reactions. After additional washes, the enzyme SβG is introduced. The beads are washed, resuspended in fluorogenic RGP and loaded into a 216,000-microwell array for multicolour imaging.

    Journal: Nature Biomedical Engineering

    Article Title: Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19

    doi: 10.1038/s41551-020-00611-x

    Figure Lengend Snippet: Schematic of the Simoa serological assay. Plasma is incubated with four types of dye-encoded beads that are each coupled to one of four viral targets (spike, S1, RBD and nucleocapsid). IgG, IgA and IgM antibodies specific to the SARS-CoV-2 targets bind to the viral antigen-coated beads. After washing, beads are introduced to biotinylated anti-human immunoglobulin antibodies to label either IgG, IgM or IgA in the different reactions. After additional washes, the enzyme SβG is introduced. The beads are washed, resuspended in fluorogenic RGP and loaded into a 216,000-microwell array for multicolour imaging.

    Article Snippet: Anti-human immunoglobulin antibodies were diluted in Homebrew Detector/Sample Diluent to final concentrations of 7.73 ng ml−1 IgG (Bethyl Labratories; A80-148B), 216 ng ml−1 IgM (Thermo Fisher Scientific; MII0401) and 150 ng ml−1 IgA (Abcam; ab214003).

    Techniques: Serologic Assay, Incubation, Imaging

    MHC-I and MHC-II are involved in antibody isotype switching. B2m KO, MHC-II KO, and WT C57BL/6 mice were vaccinated s.c. with 10 μg of PIV and challenged i.p. with 1 × 10 7 genomic copies of C. burnetii NMI 28 dpv. Mice receiving Alhydrogel adjuvant alone served as unvaccinated controls. C. burnetii NMI-specific serum IgM (A to C) and IgG (D to F) were evaluated weekly following vaccination. Specific IgM (G) and IgG (H) were also evaluated 14 dpi. Each experimental group includes five mice, with error bars representing the standard deviations from the mean. **, P

    Journal: Infection and Immunity

    Article Title: Major Histocompatibility Complex Class II-Restricted, CD4+ T Cell-Dependent and -Independent Mechanisms Are Required for Vaccine-Induced Protective Immunity against Coxiella burnetii

    doi: 10.1128/IAI.00824-19

    Figure Lengend Snippet: MHC-I and MHC-II are involved in antibody isotype switching. B2m KO, MHC-II KO, and WT C57BL/6 mice were vaccinated s.c. with 10 μg of PIV and challenged i.p. with 1 × 10 7 genomic copies of C. burnetii NMI 28 dpv. Mice receiving Alhydrogel adjuvant alone served as unvaccinated controls. C. burnetii NMI-specific serum IgM (A to C) and IgG (D to F) were evaluated weekly following vaccination. Specific IgM (G) and IgG (H) were also evaluated 14 dpi. Each experimental group includes five mice, with error bars representing the standard deviations from the mean. **, P

    Article Snippet: Plates were washed with PBS-T buffer and then incubated with 100 μl of diluted horseradish peroxidase-conjugated goat anti-mouse IgM or IgG (1:4,000 to 1:8,000) at room temperature for 1 h. The plates were again washed with PBS-T, followed by the addition of 100 μl of TMB substrate (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Mouse Assay

    CD4 + T cells are important for antibody isotype switching. WT, CD4 KO, and MHC-II KO mice were vaccinated and challenged as previously described. Mice receiving Alhydrogel adjuvant alone served as unvaccinated controls. C. burnetii NMI-specific serum IgM and IgG were evaluated weekly until 28 dpv (A) and 14 dpi (B). Each experimental group includes five mice, with error bars representing the standard deviations from the mean. *, P

    Journal: Infection and Immunity

    Article Title: Major Histocompatibility Complex Class II-Restricted, CD4+ T Cell-Dependent and -Independent Mechanisms Are Required for Vaccine-Induced Protective Immunity against Coxiella burnetii

    doi: 10.1128/IAI.00824-19

    Figure Lengend Snippet: CD4 + T cells are important for antibody isotype switching. WT, CD4 KO, and MHC-II KO mice were vaccinated and challenged as previously described. Mice receiving Alhydrogel adjuvant alone served as unvaccinated controls. C. burnetii NMI-specific serum IgM and IgG were evaluated weekly until 28 dpv (A) and 14 dpi (B). Each experimental group includes five mice, with error bars representing the standard deviations from the mean. *, P

    Article Snippet: Plates were washed with PBS-T buffer and then incubated with 100 μl of diluted horseradish peroxidase-conjugated goat anti-mouse IgM or IgG (1:4,000 to 1:8,000) at room temperature for 1 h. The plates were again washed with PBS-T, followed by the addition of 100 μl of TMB substrate (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Mouse Assay

    The immunogenicity of M2FA-lysine-BSA in the absence of adjuvant and anti-M2FA antibody titers in intact mice. C57BL/6 mice were injected i.p. with M2FA-lysine-BSA or BSA in the absence of adjuvant. The antibody titers of IgG ( a ) and IgM ( b ) against M2FA-lysine were detected using MFA-6ACA-KLH-coated plates. The anti-M2AA antibody titers were clearly increased in M2FA-lysine-BSA-immunized mice compared to the controls (BSA-treated mice). Values are mean and SD. ( c ) Intact female C57BL/6 mice (n = 4 or 5 per group) with different ages showed significantly different anti-M2FA IgG titers. Values are mean and SD. (*p

    Journal: Scientific Reports

    Article Title: Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes

    doi: 10.1038/s41598-017-11289-8

    Figure Lengend Snippet: The immunogenicity of M2FA-lysine-BSA in the absence of adjuvant and anti-M2FA antibody titers in intact mice. C57BL/6 mice were injected i.p. with M2FA-lysine-BSA or BSA in the absence of adjuvant. The antibody titers of IgG ( a ) and IgM ( b ) against M2FA-lysine were detected using MFA-6ACA-KLH-coated plates. The anti-M2AA antibody titers were clearly increased in M2FA-lysine-BSA-immunized mice compared to the controls (BSA-treated mice). Values are mean and SD. ( c ) Intact female C57BL/6 mice (n = 4 or 5 per group) with different ages showed significantly different anti-M2FA IgG titers. Values are mean and SD. (*p

    Article Snippet: Malondialdehyde bis(dimethyl) acetal, Dynabeads M-270 Amine, CarboxyLink Kit, the Imject EDC mcKLH Spin Kit, goat anti-rabbit IgG (H + L) antibody with HRP, goat anti-human IgG/IgM secondary antibody, and goat anti-mouse IgM secondary antibody were obtained from Thermo Scientific (Rockford, IL).

    Techniques: Mouse Assay, Injection

    Serum anti-M2FA IgG and IgM antibody levels in wild-type and ApoE −/− mice and immunohistochemical detection of M2FA-epitopes in heart valve of ApoE −/− mice. The anti-M2FA IgG ( a ) and IgM ( b ) antibody levels showed significant differences between wild-type and ApoE −/− mice with the M2FA-6ACA-BSA ELISAs (**p

    Journal: Scientific Reports

    Article Title: Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes

    doi: 10.1038/s41598-017-11289-8

    Figure Lengend Snippet: Serum anti-M2FA IgG and IgM antibody levels in wild-type and ApoE −/− mice and immunohistochemical detection of M2FA-epitopes in heart valve of ApoE −/− mice. The anti-M2FA IgG ( a ) and IgM ( b ) antibody levels showed significant differences between wild-type and ApoE −/− mice with the M2FA-6ACA-BSA ELISAs (**p

    Article Snippet: Malondialdehyde bis(dimethyl) acetal, Dynabeads M-270 Amine, CarboxyLink Kit, the Imject EDC mcKLH Spin Kit, goat anti-rabbit IgG (H + L) antibody with HRP, goat anti-human IgG/IgM secondary antibody, and goat anti-mouse IgM secondary antibody were obtained from Thermo Scientific (Rockford, IL).

    Techniques: Mouse Assay, Immunohistochemistry