igg4  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Name:
    IgG4 Monoclonal Antibody
    Description:
    IgG4 Monoclonal Antibody for Western Blot IHC
    Catalog Number:
    ma524862
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher igg4
    Recognition of NH and SMT antigens in patients from a L. donovani endemic area in Bangladesh. ( a ) Total <t>IgG</t> against L. donovani SLA, NH and SMT are shown. Antibodies against the antigens were measured by ELISA in sera (used at 1:400 dilution) in VL individuals ( n =25), asymptomatic individuals ( n =32) and non-endemic controls ( n =24). ( b ) PMBCs were recalled with 10 μg ml −1 L. donovani SLA, NH or SMT and analyzed for CD4 T cells production of indicated cytokines by flow cytometry. ( a and b ) Black bars indicate median OD for each group. Statistical significance indicated is vs the non-endemic normal group; statistic was calculated by Kolmogorov–Smirnov t -test. **** P
    IgG4 Monoclonal Antibody for Western Blot IHC
    https://www.bioz.com/result/igg4/product/Thermo Fisher
    Average 96 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    igg4 - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "From mouse to man: safety, immunogenicity and efficacy of a candidate leishmaniasis vaccine LEISH-F3+GLA-SE"

    Article Title: From mouse to man: safety, immunogenicity and efficacy of a candidate leishmaniasis vaccine LEISH-F3+GLA-SE

    Journal: Clinical & Translational Immunology

    doi: 10.1038/cti.2015.6

    Recognition of NH and SMT antigens in patients from a L. donovani endemic area in Bangladesh. ( a ) Total IgG against L. donovani SLA, NH and SMT are shown. Antibodies against the antigens were measured by ELISA in sera (used at 1:400 dilution) in VL individuals ( n =25), asymptomatic individuals ( n =32) and non-endemic controls ( n =24). ( b ) PMBCs were recalled with 10 μg ml −1 L. donovani SLA, NH or SMT and analyzed for CD4 T cells production of indicated cytokines by flow cytometry. ( a and b ) Black bars indicate median OD for each group. Statistical significance indicated is vs the non-endemic normal group; statistic was calculated by Kolmogorov–Smirnov t -test. **** P
    Figure Legend Snippet: Recognition of NH and SMT antigens in patients from a L. donovani endemic area in Bangladesh. ( a ) Total IgG against L. donovani SLA, NH and SMT are shown. Antibodies against the antigens were measured by ELISA in sera (used at 1:400 dilution) in VL individuals ( n =25), asymptomatic individuals ( n =32) and non-endemic controls ( n =24). ( b ) PMBCs were recalled with 10 μg ml −1 L. donovani SLA, NH or SMT and analyzed for CD4 T cells production of indicated cytokines by flow cytometry. ( a and b ) Black bars indicate median OD for each group. Statistical significance indicated is vs the non-endemic normal group; statistic was calculated by Kolmogorov–Smirnov t -test. **** P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry

    LEISH-F3 formulated with GLA-SE is immunogenic in humans. Thirty-six healthy adult subjects were immunized on days 0, 28 and 56 with 20 μg LEISH-F3+2 μg GLA-SE ( n =12), 20 μg LEISH-F3+5 μg GLA-SE ( n =12) and 20μg LEISH-F3 protein alone ( n =12). The immunogenicity of the vaccine was evaluated by assessing antibody and T-cell responses at days 0, 35, 63, 84 and 168 and days 0, 7, 35, 63, 84 and 168, respectively. ( a and b ) ELISAs for titers of LEISH-F3-specific antibodies (total and IgG subclasses and total IgE) in volunteer serum were conducted for the indicated time points. ( c ) Quantitative T-cell responses to LEISH-F3 was measured by IL-2, IL-5, IL-10, IFN-γ and TNF cytokine production in whole blood Luminex assay (WBA). Nine subjects were excluded from the per-protocol population immunology summaries resulting in evaluable groups such as: 2 μg GLA-SE ( n =8), 5 μg GLA-SE ( n =10) and LEISH-F3 alone ( n =9). P -value for comparison was performed for various treatment groups: between 2 and 5 μg GLA-SE vaccine groups and between vaccine (2 and 5 μg GLA-SE vaccine groups combined) and 20 μg LEISH-F3 alone. P -values were considered significant at the 0.05 significance level. * P -values significant at 0.05 significance level when compared between vaccine (2 and 5 μg GLA-SE vaccine groups combined) and 20 μg LEISH-F3 alone. ** P -values significant at 0.05 significance level when compared between vaccine (2 and 5 μg GLA-SE vaccine groups separately).
    Figure Legend Snippet: LEISH-F3 formulated with GLA-SE is immunogenic in humans. Thirty-six healthy adult subjects were immunized on days 0, 28 and 56 with 20 μg LEISH-F3+2 μg GLA-SE ( n =12), 20 μg LEISH-F3+5 μg GLA-SE ( n =12) and 20μg LEISH-F3 protein alone ( n =12). The immunogenicity of the vaccine was evaluated by assessing antibody and T-cell responses at days 0, 35, 63, 84 and 168 and days 0, 7, 35, 63, 84 and 168, respectively. ( a and b ) ELISAs for titers of LEISH-F3-specific antibodies (total and IgG subclasses and total IgE) in volunteer serum were conducted for the indicated time points. ( c ) Quantitative T-cell responses to LEISH-F3 was measured by IL-2, IL-5, IL-10, IFN-γ and TNF cytokine production in whole blood Luminex assay (WBA). Nine subjects were excluded from the per-protocol population immunology summaries resulting in evaluable groups such as: 2 μg GLA-SE ( n =8), 5 μg GLA-SE ( n =10) and LEISH-F3 alone ( n =9). P -value for comparison was performed for various treatment groups: between 2 and 5 μg GLA-SE vaccine groups and between vaccine (2 and 5 μg GLA-SE vaccine groups combined) and 20 μg LEISH-F3 alone. P -values were considered significant at the 0.05 significance level. * P -values significant at 0.05 significance level when compared between vaccine (2 and 5 μg GLA-SE vaccine groups combined) and 20 μg LEISH-F3 alone. ** P -values significant at 0.05 significance level when compared between vaccine (2 and 5 μg GLA-SE vaccine groups separately).

    Techniques Used: Luminex

    2) Product Images from "Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG"

    Article Title: Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0016741

    Specific IgE, IgG 1 and IgG 4 over a 2 year period from 10 patients undergoing VIT. A. Median values of JJAV and Myr p 2a. B. Specific antibody levels.
    Figure Legend Snippet: Specific IgE, IgG 1 and IgG 4 over a 2 year period from 10 patients undergoing VIT. A. Median values of JJAV and Myr p 2a. B. Specific antibody levels.

    Techniques Used:

    3) Product Images from "Changes in N-glycans of IgG4 and its relationship with the existence of hypocomplementemia and individual organ involvement in patients with IgG4-related disease"

    Article Title: Changes in N-glycans of IgG4 and its relationship with the existence of hypocomplementemia and individual organ involvement in patients with IgG4-related disease

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0196163

    Comparison of absolute concentration of IgG4 F0 and F1 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.
    Figure Legend Snippet: Comparison of absolute concentration of IgG4 F0 and F1 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.

    Techniques Used: Concentration Assay, MANN-WHITNEY

    Comparison of absolute concentrations of IgG4 G0 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.
    Figure Legend Snippet: Comparison of absolute concentrations of IgG4 G0 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.

    Techniques Used: MANN-WHITNEY

    Comparison of absolute concentration of IgG4 G0 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was not performed.
    Figure Legend Snippet: Comparison of absolute concentration of IgG4 G0 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was not performed.

    Techniques Used: Concentration Assay

    Comparison of absolute concentration of IgG4 G2 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.
    Figure Legend Snippet: Comparison of absolute concentration of IgG4 G2 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.

    Techniques Used: Concentration Assay, MANN-WHITNEY

    Comparison of absolute concentration of IgG4 G1 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.
    Figure Legend Snippet: Comparison of absolute concentration of IgG4 G1 glycans between IgG4RD and healthy controls. Data are shown as box plots. The boxes indicate the upper and lower interquartile range (IQR), the lines within the boxes indicate the median, and the whiskers indicate the minimum and maximum IQR. Each dot represents an individual sample. Statistical analysis was performed using the Mann-Whitney U -test.

    Techniques Used: Concentration Assay, MANN-WHITNEY

    Structures of afucosylated (F0) and fucosylated (F1) glycans released from IgG4 isolated from the sera of patients with IgG4RD and healthy controls. GlcNAc: N-Acetylglucosamine, GalNAc: N-Acetylgalactosamine.
    Figure Legend Snippet: Structures of afucosylated (F0) and fucosylated (F1) glycans released from IgG4 isolated from the sera of patients with IgG4RD and healthy controls. GlcNAc: N-Acetylglucosamine, GalNAc: N-Acetylgalactosamine.

    Techniques Used: Isolation

    Structures of agalactosylated (G0), monogalactosylated (G1), digalactosylated (G2), and trigalactosylated (G3) glycans released from IgG4 isolated from the sera of patients with IgG4RD and healthy controls. GlcNAc: N-Acetylglucosamine, GalNAc: N-Acetylgalactosamine.
    Figure Legend Snippet: Structures of agalactosylated (G0), monogalactosylated (G1), digalactosylated (G2), and trigalactosylated (G3) glycans released from IgG4 isolated from the sera of patients with IgG4RD and healthy controls. GlcNAc: N-Acetylglucosamine, GalNAc: N-Acetylgalactosamine.

    Techniques Used: Isolation

    IgG4 isolated from the sera of patients with IgG4-related disease. (A) SDS-PAGE analysis of isolated IgG4. Bands of marker proteins over 75 kDa are blurry and unclear. (B) Western blot analysis of anti-IgG subclasses reacting with isolated IgG4. Blot reactivities for anti IgG4 antibody were observed in all samples.
    Figure Legend Snippet: IgG4 isolated from the sera of patients with IgG4-related disease. (A) SDS-PAGE analysis of isolated IgG4. Bands of marker proteins over 75 kDa are blurry and unclear. (B) Western blot analysis of anti-IgG subclasses reacting with isolated IgG4. Blot reactivities for anti IgG4 antibody were observed in all samples.

    Techniques Used: Isolation, SDS Page, Marker, Western Blot

    Structures of asialylated (S0), monosialylated (S1), and disialylated (S2) glycans released from IgG4 isolated from the sera of patients with IgG4RD and healthy controls. GlcNAc: N-Acetylglucosamine, GalNAc: N-Acetylgalactosamine.
    Figure Legend Snippet: Structures of asialylated (S0), monosialylated (S1), and disialylated (S2) glycans released from IgG4 isolated from the sera of patients with IgG4RD and healthy controls. GlcNAc: N-Acetylglucosamine, GalNAc: N-Acetylgalactosamine.

    Techniques Used: Isolation

    4) Product Images from "A prospective study comparing the efficacy and safety of two sublingual birch allergen preparations"

    Article Title: A prospective study comparing the efficacy and safety of two sublingual birch allergen preparations

    Journal: Clinical and Translational Allergy

    doi: 10.1186/2045-7022-4-23

    Bet v specific IgG levels (including standard error) before and after SLIT treatment. Bet v specific IgG levels increased in both groups, the increase in the SUB-B groups was significantly higher than in the Stal-B group (p = 0.03).
    Figure Legend Snippet: Bet v specific IgG levels (including standard error) before and after SLIT treatment. Bet v specific IgG levels increased in both groups, the increase in the SUB-B groups was significantly higher than in the Stal-B group (p = 0.03).

    Techniques Used:

    Bet v and Bet v1 specific IgG 4 levels (including standard error) before and after SLIT treatment. No significant differences in the increase in Bet v (p = 0.17) and Bet v 1 (p = 0.11) specific IgG4 levels was observed following SUB-B and Stal-B treatment.
    Figure Legend Snippet: Bet v and Bet v1 specific IgG 4 levels (including standard error) before and after SLIT treatment. No significant differences in the increase in Bet v (p = 0.17) and Bet v 1 (p = 0.11) specific IgG4 levels was observed following SUB-B and Stal-B treatment.

    Techniques Used:

    5) Product Images from "Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis"

    Article Title: Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

    Journal: The American Journal of Tropical Medicine and Hygiene

    doi: 10.4269/ajtmh.14-0170

    Representative native AgB dipsticks probed with colloidal gold-conjugated anti-human IgG. Lanes 1–3 show positive test results with serum samples from individual CE patients. Lane 4 shows a negative test result with a serum sample from a healthy
    Figure Legend Snippet: Representative native AgB dipsticks probed with colloidal gold-conjugated anti-human IgG. Lanes 1–3 show positive test results with serum samples from individual CE patients. Lane 4 shows a negative test result with a serum sample from a healthy

    Techniques Used:

    Representative AgB dipsticks probed with colloidal gold-conjugated anti-human IgG4. Lanes 1–3 show positive test results with serum samples from individual CE patients. Lane 4 shows a negative test result with a serum sample from a healthy individual.
    Figure Legend Snippet: Representative AgB dipsticks probed with colloidal gold-conjugated anti-human IgG4. Lanes 1–3 show positive test results with serum samples from individual CE patients. Lane 4 shows a negative test result with a serum sample from a healthy individual.

    Techniques Used:

    6) Product Images from "Utility of component analyses in subjects undergoing sublingual immunotherapy for peanut allergy"

    Article Title: Utility of component analyses in subjects undergoing sublingual immunotherapy for peanut allergy

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    doi: 10.1111/cea.12635

    Component- and peanut-specific immunoglobulin data over time on treatment for all subjects. A) Specific IgE; B) Specific IgG4; C) Specific IgE/specific IgG4. *p
    Figure Legend Snippet: Component- and peanut-specific immunoglobulin data over time on treatment for all subjects. A) Specific IgE; B) Specific IgG4; C) Specific IgE/specific IgG4. *p

    Techniques Used:

    Baseline component- and peanut-specific immunoglobulin data for subjects passing (P) and failing (F) the 12 month food challenge. A) Specific IgE; B) Specific IgG4; C) Specific IgE/Specific IgG4. *p
    Figure Legend Snippet: Baseline component- and peanut-specific immunoglobulin data for subjects passing (P) and failing (F) the 12 month food challenge. A) Specific IgE; B) Specific IgG4; C) Specific IgE/Specific IgG4. *p

    Techniques Used:

    7) Product Images from "Serum exosomes and cytokines promote a T-helper cell type 2 environment in the peripheral blood of glioblastoma patients"

    Article Title: Serum exosomes and cytokines promote a T-helper cell type 2 environment in the peripheral blood of glioblastoma patients

    Journal: Neuro-Oncology

    doi: 10.1093/neuonc/nov107

    Exosomes and soluble sera fractions from GBM patients induce M2 monocyte differentiation of normal human monocytes that correlates with levels of tumor exosome-specific sera IgG and quantities of circulating exosomes. GBM patients were divided into groups
    Figure Legend Snippet: Exosomes and soluble sera fractions from GBM patients induce M2 monocyte differentiation of normal human monocytes that correlates with levels of tumor exosome-specific sera IgG and quantities of circulating exosomes. GBM patients were divided into groups

    Techniques Used:

    Sera from GBM patient with high levels of tumor antigen (Ag)-specific sera IgG induce M2 monocyte differentiation of normal blood monocytes. GBM patients were divided into groups based on levels of tumor Ag-specific sera IgG. (A) Primary GBM cell line
    Figure Legend Snippet: Sera from GBM patient with high levels of tumor antigen (Ag)-specific sera IgG induce M2 monocyte differentiation of normal blood monocytes. GBM patients were divided into groups based on levels of tumor Ag-specific sera IgG. (A) Primary GBM cell line

    Techniques Used:

    8) Product Images from "18F-fluoro-deoxyglucose positron emission tomography/computed tomography scan findings in Rosai-Dorfman disease with IgG4-positive plasma cell infiltration mimicking breast malignancy: a case report and literature review"

    Article Title: 18F-fluoro-deoxyglucose positron emission tomography/computed tomography scan findings in Rosai-Dorfman disease with IgG4-positive plasma cell infiltration mimicking breast malignancy: a case report and literature review

    Journal: Journal of Medical Case Reports

    doi: 10.1186/1752-1947-6-411

    Maximum intensity projection image of a 78-year-old woman with Rosai-Dorfman disease accompanied by IgG4 + plasma cell infiltration. Besides the hypermetabolic lesion of the right breast, mild 18 F-fluoro-deoxyglucose uptake was illustrated at the hila of the lungs and neck bilaterally.
    Figure Legend Snippet: Maximum intensity projection image of a 78-year-old woman with Rosai-Dorfman disease accompanied by IgG4 + plasma cell infiltration. Besides the hypermetabolic lesion of the right breast, mild 18 F-fluoro-deoxyglucose uptake was illustrated at the hila of the lungs and neck bilaterally.

    Techniques Used:

    Histopathological images and immunohistochemical staining of the mammary lesion. ( A ) High-power photomicrograph shows distinctive histiocytes of Rosai-Dorfman disease that are large with abundant and finely granular pink cytoplasm and relatively large nuclei with open chromatin and distinct nucleoli. Engulfment of intact lymphocytes and plasma cells by the large histiocytes, a phenomenon known as emperipolesis (arrow), is shown (stain: hematoxylin-eosin; original magnification: ×400). ( B ) Immunohistochemical staining for IgG4 labels most of the plasma cells (IgG immunohistochemical stain counterstained by hematoxylin-eosin; original magnification: ×400).
    Figure Legend Snippet: Histopathological images and immunohistochemical staining of the mammary lesion. ( A ) High-power photomicrograph shows distinctive histiocytes of Rosai-Dorfman disease that are large with abundant and finely granular pink cytoplasm and relatively large nuclei with open chromatin and distinct nucleoli. Engulfment of intact lymphocytes and plasma cells by the large histiocytes, a phenomenon known as emperipolesis (arrow), is shown (stain: hematoxylin-eosin; original magnification: ×400). ( B ) Immunohistochemical staining for IgG4 labels most of the plasma cells (IgG immunohistochemical stain counterstained by hematoxylin-eosin; original magnification: ×400).

    Techniques Used: Immunohistochemistry, Staining

    9) Product Images from "IgG4-Related Disease Is Not Associated with Antibody to the Phospholipase A2 Receptor"

    Article Title: IgG4-Related Disease Is Not Associated with Antibody to the Phospholipase A2 Receptor

    Journal: International Journal of Rheumatology

    doi: 10.1155/2012/139409

    Representative immunoblot demonstrating that sera from patients with IgG4-RD (lanes 2–5) lack detectable reactivity with rPLA2R. Serum from a patient with idiopathic membranous nephropathy (lane 1) was used as a positive control.
    Figure Legend Snippet: Representative immunoblot demonstrating that sera from patients with IgG4-RD (lanes 2–5) lack detectable reactivity with rPLA2R. Serum from a patient with idiopathic membranous nephropathy (lane 1) was used as a positive control.

    Techniques Used: Positive Control

    10) Product Images from "Blocking antibodies induced by peanut oral and sublingual immunotherapy suppress basophil activation and are associated with sustained unresponsiveness"

    Article Title: Blocking antibodies induced by peanut oral and sublingual immunotherapy suppress basophil activation and are associated with sustained unresponsiveness

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    doi: 10.1111/cea.13305

    Inhibition capacity of IgG-depleted plasma or the IgG fraction isolated from plasma. Percent CD63+ basophils following incubation with undiluted, sham-depleted, or IgG-depleted 0 month or 12 month OIT plasma and stimulation with peanut extract (A). Percent CD63+ basophils following incubation with undiluted, IgG-depleted or IgG fractions from 18 month OIT plasma and stimulation with peanut extract (B). Individual data are shown; red lines indicate medians; *p
    Figure Legend Snippet: Inhibition capacity of IgG-depleted plasma or the IgG fraction isolated from plasma. Percent CD63+ basophils following incubation with undiluted, sham-depleted, or IgG-depleted 0 month or 12 month OIT plasma and stimulation with peanut extract (A). Percent CD63+ basophils following incubation with undiluted, IgG-depleted or IgG fractions from 18 month OIT plasma and stimulation with peanut extract (B). Individual data are shown; red lines indicate medians; *p

    Techniques Used: Inhibition, Isolation, Incubation

    11) Product Images from "Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure"

    Article Title: Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure

    Journal: BMC Medicine

    doi: 10.1186/s12916-019-1277-x

    Relationship between age and immunity. Children in RTS,S vaccine group from Manhiça (black box plots) and Ilha Josina cohorts (gray box plots) were categorized into younger (12 to 24 months; Manhiça n = 11 and Ilha Josina n = 23, respectively) and older (24 to 60 months; Manhiça n = 39 and Ilha Josina n = 26, respectively) age groups. Sera collected after vaccination (month 3, M3) were tested for C1q-fixation to CSP and NANP ( a ) and IgG-reactivity to blood-stage antigens MSP2 and AMA1 ( b ). Samples were tested in duplicate, and the mean value was used to generate box plots for samples stratified by age group. Top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between unpaired samples was compared using Mann-Whitney U test
    Figure Legend Snippet: Relationship between age and immunity. Children in RTS,S vaccine group from Manhiça (black box plots) and Ilha Josina cohorts (gray box plots) were categorized into younger (12 to 24 months; Manhiça n = 11 and Ilha Josina n = 23, respectively) and older (24 to 60 months; Manhiça n = 39 and Ilha Josina n = 26, respectively) age groups. Sera collected after vaccination (month 3, M3) were tested for C1q-fixation to CSP and NANP ( a ) and IgG-reactivity to blood-stage antigens MSP2 and AMA1 ( b ). Samples were tested in duplicate, and the mean value was used to generate box plots for samples stratified by age group. Top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between unpaired samples was compared using Mann-Whitney U test

    Techniques Used: MANN-WHITNEY

    RTS,S vaccine-induced immunity declines over time. A random selection of children vaccinated with RTS,S (Manhiça cohort, n = 30) was tested for C1q-fixation ( a ), IgG/IgM ( b ), and IgG subclasses ( c ) to CSP at months 3, 8.5, 21, 33, 45, and 6. Note that due to low reactivity, C1q-fixation was re-tested at a higher dilution of 1/110, in addition to 1/250, to confirm results. Samples were tested in duplicate, and the median and 95% CI of the median from each time point group are shown by the symbol and shaded area, respectively
    Figure Legend Snippet: RTS,S vaccine-induced immunity declines over time. A random selection of children vaccinated with RTS,S (Manhiça cohort, n = 30) was tested for C1q-fixation ( a ), IgG/IgM ( b ), and IgG subclasses ( c ) to CSP at months 3, 8.5, 21, 33, 45, and 6. Note that due to low reactivity, C1q-fixation was re-tested at a higher dilution of 1/110, in addition to 1/250, to confirm results. Samples were tested in duplicate, and the median and 95% CI of the median from each time point group are shown by the symbol and shaded area, respectively

    Techniques Used: Selection

    RTS,S vaccine-induced IgG and IgM antibodies to CSP. Children in RTS,S and comparator vaccine groups from Manhiça (black box plots; N = 50 and N = 25, respectively) and Ilha Josina cohorts (gray box plots; N = 49 and N = 24, respectively) were tested for IgG ( a ), IgG subclasses ( b ), and IgM ( c ) to CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate (note that only M3 was tested in b ), and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff value (dashed lines), and the percentage of individuals above this threshold are shown. Reactivity between paired samples and unpaired samples were compared using Wilcoxon matched-pairs signed-rank test and Mann-Whitney U test, respectively
    Figure Legend Snippet: RTS,S vaccine-induced IgG and IgM antibodies to CSP. Children in RTS,S and comparator vaccine groups from Manhiça (black box plots; N = 50 and N = 25, respectively) and Ilha Josina cohorts (gray box plots; N = 49 and N = 24, respectively) were tested for IgG ( a ), IgG subclasses ( b ), and IgM ( c ) to CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate (note that only M3 was tested in b ), and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff value (dashed lines), and the percentage of individuals above this threshold are shown. Reactivity between paired samples and unpaired samples were compared using Wilcoxon matched-pairs signed-rank test and Mann-Whitney U test, respectively

    Techniques Used: MANN-WHITNEY

    High variability among RTS,S vaccine-induced IgG targeting the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, 3 M) were tested for IgG to NANP and CT and C1q-fixation to CSP, and the values were used for the following analysis. a Heat map of children arranged in descending (top to bottom) order of C1q-fixation (left), corresponding IgG to NANP and CT (middle), and for comparison IgM to NANP and CT (right). b Variability between epitope specificity was quantified by calculating the ratio of NANP-to-CT IgG. Children with low variability were considered to have equal reactivity to NANP and CT (ratio between 0.75 and 1.25 shown in white), and children exceeding this range were considered to have a NANP- or CT-skewed response (ratio > 1.25 shown in purple and ratio
    Figure Legend Snippet: High variability among RTS,S vaccine-induced IgG targeting the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, 3 M) were tested for IgG to NANP and CT and C1q-fixation to CSP, and the values were used for the following analysis. a Heat map of children arranged in descending (top to bottom) order of C1q-fixation (left), corresponding IgG to NANP and CT (middle), and for comparison IgM to NANP and CT (right). b Variability between epitope specificity was quantified by calculating the ratio of NANP-to-CT IgG. Children with low variability were considered to have equal reactivity to NANP and CT (ratio between 0.75 and 1.25 shown in white), and children exceeding this range were considered to have a NANP- or CT-skewed response (ratio > 1.25 shown in purple and ratio

    Techniques Used:

    Functional complement-fixing antibodies target the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça (black box plots; N = 50) and Ilha Josina cohorts (gray box plots; N = 49) were tested for IgG ( a ) and IgM ( b ) to NANP and CT regions of CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate, and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between paired samples was compared using Wilcoxon matched-pairs signed-rank test. c , d Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, M3) were tested for C1q-fixation to CSP, NANP, and CT, and the values were plotted compared to IgG reactivity (c) and C1q fixation to CSP, NANP, and CT were correlated (d)
    Figure Legend Snippet: Functional complement-fixing antibodies target the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça (black box plots; N = 50) and Ilha Josina cohorts (gray box plots; N = 49) were tested for IgG ( a ) and IgM ( b ) to NANP and CT regions of CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate, and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between paired samples was compared using Wilcoxon matched-pairs signed-rank test. c , d Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, M3) were tested for C1q-fixation to CSP, NANP, and CT, and the values were plotted compared to IgG reactivity (c) and C1q fixation to CSP, NANP, and CT were correlated (d)

    Techniques Used: Functional Assay

    12) Product Images from "Placental gene expression and antibody levels of mother-neonate pairs reveal an enhanced risk for inflammation in a helminth endemic country"

    Article Title: Placental gene expression and antibody levels of mother-neonate pairs reveal an enhanced risk for inflammation in a helminth endemic country

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52074-z

    Maternal and fetal plasma IgG4 levels and their correlation in Gabon and Germany. IgG4 levels were measured in plasma via ELISA. All data are shown with median and interquartile range. P values are for Mann-Whitney U-tests or Spearman’s rank correlation, respectively. Comparison between the two maternal, or cord blood groups, respectively. In the case additionally comparison between maternal and cord blood group each to show the placental transfer; P value: *
    Figure Legend Snippet: Maternal and fetal plasma IgG4 levels and their correlation in Gabon and Germany. IgG4 levels were measured in plasma via ELISA. All data are shown with median and interquartile range. P values are for Mann-Whitney U-tests or Spearman’s rank correlation, respectively. Comparison between the two maternal, or cord blood groups, respectively. In the case additionally comparison between maternal and cord blood group each to show the placental transfer; P value: *

    Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    13) Product Images from "Molecular patterns in the isotype-specific antibody responses to the major cedar aeroallergen Jun a 1"

    Article Title: Molecular patterns in the isotype-specific antibody responses to the major cedar aeroallergen Jun a 1

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2018.08.007

    Titer of Jun a 1-specific IgE, IgG and IgA antibodies are shown with their mean ± SD (open bars). IgG4 antibodies were not shown in this figure because of their very low titers. Significance differences in the titer of different isotypes are indicated by * P
    Figure Legend Snippet: Titer of Jun a 1-specific IgE, IgG and IgA antibodies are shown with their mean ± SD (open bars). IgG4 antibodies were not shown in this figure because of their very low titers. Significance differences in the titer of different isotypes are indicated by * P

    Techniques Used:

    14) Product Images from "Characterisation of CD154+ T cells following ex vivo birch allergen stimulation defines a close relationship between T cell subsets in healthy volunteers"

    Article Title: Characterisation of CD154+ T cells following ex vivo birch allergen stimulation defines a close relationship between T cell subsets in healthy volunteers

    Journal: BMC Immunology

    doi: 10.1186/1471-2172-14-14

    Background-corrected birch-allergen induced cytokine expression in non-allergics (IL4-responders vs. IL4 non-responders). Data represents ( a ) CD154 + IL-4 + , ( b ) CD154 + , ( c ) CD154 + IFNγ + , ( d ) CD154 + IL-10 + illustrated as the frequency of positive cells per 10 6 CD4 T cells on a log scale, ( e ) Th2:Th1 ratio and ( f ) IgG4 levels in birch-allergic IL-10 responders vs. IL-10 non-responders.
    Figure Legend Snippet: Background-corrected birch-allergen induced cytokine expression in non-allergics (IL4-responders vs. IL4 non-responders). Data represents ( a ) CD154 + IL-4 + , ( b ) CD154 + , ( c ) CD154 + IFNγ + , ( d ) CD154 + IL-10 + illustrated as the frequency of positive cells per 10 6 CD4 T cells on a log scale, ( e ) Th2:Th1 ratio and ( f ) IgG4 levels in birch-allergic IL-10 responders vs. IL-10 non-responders.

    Techniques Used: Expressing

    15) Product Images from "Similar Humoral and Cellular Immunological Reactivities to Human Herpesvirus 6 in Patients with Multiple Sclerosis and Controls"

    Article Title: Similar Humoral and Cellular Immunological Reactivities to Human Herpesvirus 6 in Patients with Multiple Sclerosis and Controls

    Journal: Clinical and Diagnostic Laboratory Immunology

    doi:

    Distribution in serum of detectable subclasses of IgG to HHV-6 (a) and EBV (b) in patients with MS (heavy shading) and controls (light shading). Percentages are at the top of each column.
    Figure Legend Snippet: Distribution in serum of detectable subclasses of IgG to HHV-6 (a) and EBV (b) in patients with MS (heavy shading) and controls (light shading). Percentages are at the top of each column.

    Techniques Used: Mass Spectrometry

    16) Product Images from "Absence of SpeB Production in Virulent Large Capsular Forms of Group A Streptococcal Strain 64"

    Article Title: Absence of SpeB Production in Virulent Large Capsular Forms of Group A Streptococcal Strain 64

    Journal: Infection and Immunity

    doi:

    Treatment of recombinant Emm, Mrp, or Enn 64 with a streptococcal cysteine protease and effect on IgG-binding properties. Sonicates of E. coli expressing recombinant Emm, Mrp, or Enn protein were incubated at 37°C for the times indicated with the cysteine protease prepared from the culture supernatant of strain 64p. The reaction was stopped by addition of sample buffer and boiling. The immunoglobulin reactivity remaining following immunoblotting was measured as described in Materials and Methods. Blots were developed by an ECL procedure and exposed to Kodak XAR film for 30 to 60 s.
    Figure Legend Snippet: Treatment of recombinant Emm, Mrp, or Enn 64 with a streptococcal cysteine protease and effect on IgG-binding properties. Sonicates of E. coli expressing recombinant Emm, Mrp, or Enn protein were incubated at 37°C for the times indicated with the cysteine protease prepared from the culture supernatant of strain 64p. The reaction was stopped by addition of sample buffer and boiling. The immunoglobulin reactivity remaining following immunoblotting was measured as described in Materials and Methods. Blots were developed by an ECL procedure and exposed to Kodak XAR film for 30 to 60 s.

    Techniques Used: Recombinant, Binding Assay, Expressing, Incubation

    17) Product Images from "Detection of Mycoplasma genitalium-Reactive Cervicovaginal Antibodies among Infected Women ▿"

    Article Title: Detection of Mycoplasma genitalium-Reactive Cervicovaginal Antibodies among Infected Women ▿

    Journal: Clinical and Vaccine Immunology : CVI

    doi: 10.1128/CVI.05174-11

    M. genitalium infection elicits IgG1 and IgG3 subtypes. A subset of strongly IgG-positive samples was characterized further using subtype-specific secondary antibodies. The results from two IgG-positive cases are shown. Cervical and vaginal samples for
    Figure Legend Snippet: M. genitalium infection elicits IgG1 and IgG3 subtypes. A subset of strongly IgG-positive samples was characterized further using subtype-specific secondary antibodies. The results from two IgG-positive cases are shown. Cervical and vaginal samples for

    Techniques Used: Infection

    18) Product Images from "Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies"

    Article Title: Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies

    Journal: Malaria Journal

    doi: 10.1186/s12936-018-2369-3

    RTS,S-specific responses measured in the WHO reference reagent, IgM pool and samples from RTS,S-vaccinated children. The 3 samples from RTS,S vaccinated children were of high, medium and low CSP IgG titres. a – g IgG, IgG 1–4 , IgM and IgE levels to RTS,S-specific antigens measured in the WHO reference reagent; IgG, IgG 1, IgG2 and IgG4 also measured in RTS,S-vaccinated children; h IgM levels to RTS,S-specific antigens measured in the IgM pool vs. RTS,S-vaccinated children. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG 1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S vaccinees samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG 1–4 ; and 1:2 starting at 1:10 for IgE). Isolated dots represent the levels measured in the technical blanks
    Figure Legend Snippet: RTS,S-specific responses measured in the WHO reference reagent, IgM pool and samples from RTS,S-vaccinated children. The 3 samples from RTS,S vaccinated children were of high, medium and low CSP IgG titres. a – g IgG, IgG 1–4 , IgM and IgE levels to RTS,S-specific antigens measured in the WHO reference reagent; IgG, IgG 1, IgG2 and IgG4 also measured in RTS,S-vaccinated children; h IgM levels to RTS,S-specific antigens measured in the IgM pool vs. RTS,S-vaccinated children. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG 1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S vaccinees samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG 1–4 ; and 1:2 starting at 1:10 for IgE). Isolated dots represent the levels measured in the technical blanks

    Techniques Used: Isolation

    IgG, IgG 1–4 and IgM fitted curves using the WHO-CSP pool to the 40-antigen multiplex panel incubating at 4 °C ON. Lines and dots represent predicted levels from 5PL, 4PL or exponential regression equations from 23 titration curves for IgG, IgG1, IgG3 and IgM; and 12 curves for IgG2 and IgG4. Titration curves contained 18 serial dilutions (1:2) starting at 1/50 of the WHO-CSP pool to a panel of 39 P. falciparum antigens plus HBsAg, α-Gal, BSA and GST
    Figure Legend Snippet: IgG, IgG 1–4 and IgM fitted curves using the WHO-CSP pool to the 40-antigen multiplex panel incubating at 4 °C ON. Lines and dots represent predicted levels from 5PL, 4PL or exponential regression equations from 23 titration curves for IgG, IgG1, IgG3 and IgM; and 12 curves for IgG2 and IgG4. Titration curves contained 18 serial dilutions (1:2) starting at 1/50 of the WHO-CSP pool to a panel of 39 P. falciparum antigens plus HBsAg, α-Gal, BSA and GST

    Techniques Used: Multiplex Assay, Titration

    Levels of IgG1 measured to 15 antigens in the WHO reference reagent compared to negative control and blanks under three different incubation conditions. Curve plots of the antigen-specific IgG1 levels measured in serial dilutions of the WHO reference reagent, negative control and blanks at three different incubation conditions: 37 °C 2 h, 4 °C overnight (4 °C ON) and room temperature 1 h (RT 1 h). “neg” means negative control
    Figure Legend Snippet: Levels of IgG1 measured to 15 antigens in the WHO reference reagent compared to negative control and blanks under three different incubation conditions. Curve plots of the antigen-specific IgG1 levels measured in serial dilutions of the WHO reference reagent, negative control and blanks at three different incubation conditions: 37 °C 2 h, 4 °C overnight (4 °C ON) and room temperature 1 h (RT 1 h). “neg” means negative control

    Techniques Used: Negative Control, Incubation

    Boxplots of ratios of IgG 1–4 subclasses to total IgG measured in the WHO-CSP pool. Ratios are composed with the median of the 23 titration curves for IgG, IgG1 and IgG3 and 12 curves for IgG2 and IgG4, for each dilution point. Boxes show medians and interquartile ranges. The red star corresponds to the ratio of the median of each dilution of IgG subclass to the median of each dilution of total IgG
    Figure Legend Snippet: Boxplots of ratios of IgG 1–4 subclasses to total IgG measured in the WHO-CSP pool. Ratios are composed with the median of the 23 titration curves for IgG, IgG1 and IgG3 and 12 curves for IgG2 and IgG4, for each dilution point. Boxes show medians and interquartile ranges. The red star corresponds to the ratio of the median of each dilution of IgG subclass to the median of each dilution of total IgG

    Techniques Used: Titration

    19) Product Images from "Differential Plasma-cell evolution is linked with Dermatophagoides pteronyssinus immunotherapy response"

    Article Title: Differential Plasma-cell evolution is linked with Dermatophagoides pteronyssinus immunotherapy response

    Journal: Scientific Reports

    doi: 10.1038/srep14482

    Co-evolution of Plasma-cells with IgG4 secreting cells in CG, RP and NRP. ( A ) Changes in percentage of IgG4 secreting cells over time determined by ELISpot showed an inverse evolution to the changes in the percentage of inflammatory plasma-cells (CXCR3 + ). On the contrary, the same evolution pattern was found in CXCR3 - CXCR4 - plasma-cells in RP patients only. This result suggests the possibility they may be the same cells. ( B ) Box plots representing the median and IQRs of the percentages of CXCR3 - CXCR4 - plasma-cells for each time point. An increase of these cells was observed in RP patients during AIT. Statistical Wilcoxon and Mann-Whitney U tests were performed and, applying Bonferroni correction, significant changes were considered when p
    Figure Legend Snippet: Co-evolution of Plasma-cells with IgG4 secreting cells in CG, RP and NRP. ( A ) Changes in percentage of IgG4 secreting cells over time determined by ELISpot showed an inverse evolution to the changes in the percentage of inflammatory plasma-cells (CXCR3 + ). On the contrary, the same evolution pattern was found in CXCR3 - CXCR4 - plasma-cells in RP patients only. This result suggests the possibility they may be the same cells. ( B ) Box plots representing the median and IQRs of the percentages of CXCR3 - CXCR4 - plasma-cells for each time point. An increase of these cells was observed in RP patients during AIT. Statistical Wilcoxon and Mann-Whitney U tests were performed and, applying Bonferroni correction, significant changes were considered when p

    Techniques Used: Enzyme-linked Immunospot, MANN-WHITNEY

    Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by ImmunoCAP-FEIA in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.
    Figure Legend Snippet: Evolution of Ig serum levels and IgG4 secreting cells in CG, RP and NRP. Ig levels in the different time points of the study were measured at the same time at the end of the first year of treatment. ( A ) Evolution of Total IgE (Ku/L). ( B ) Ratio between spIgE and spIgG4 in the three groups of patients represented as means and SD showing a significant decrease in RP from the first month of treatment. ( C ) Evolution of the percentage of IgG4 secreting cells determined by ELISpot and IgG4 serum levels determined by ImmunoCAP-FEIA in (C1) Controls, (C2) NRP and (C3) RP, where an increase of both parameters from the first month of AIT was observed. Statistical Wilcoxon and Mann-Whitney U tests were performed. The Bonferroni correction was applied for comparison of three groups and five different time points.

    Techniques Used: Enzyme-linked Immunospot, MANN-WHITNEY

    Proposed model representing the B cell subtypes involved in the development of the AR. In first contact, the allergen is presented to naïve B-cells; these activate and begin somatic hypermutation and class-switch recombination. Some of them become short-lived plasma-cells able to secrete low-affinity IgE as the first step of immunological protection. Another subset of activated B-cells becomes Memory B-cells. In successive contact with the allergen the memory B-cells differentiate into plasmablast that are able to secrete spIgE and proliferate, differentiating into: Long-lived plasma-cells, that preferentially recirculate to Bone Marrow, and Inflammatory plasma-cells, that are recruited to the peripheral tissues and act as the real effector cells with the secretion of spIgE. This proposed model is based on our current knowledge of IgG responses.
    Figure Legend Snippet: Proposed model representing the B cell subtypes involved in the development of the AR. In first contact, the allergen is presented to naïve B-cells; these activate and begin somatic hypermutation and class-switch recombination. Some of them become short-lived plasma-cells able to secrete low-affinity IgE as the first step of immunological protection. Another subset of activated B-cells becomes Memory B-cells. In successive contact with the allergen the memory B-cells differentiate into plasmablast that are able to secrete spIgE and proliferate, differentiating into: Long-lived plasma-cells, that preferentially recirculate to Bone Marrow, and Inflammatory plasma-cells, that are recruited to the peripheral tissues and act as the real effector cells with the secretion of spIgE. This proposed model is based on our current knowledge of IgG responses.

    Techniques Used: Activated Clotting Time Assay

    20) Product Images from "Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccination parameters"

    Article Title: Humoral anti-KLH responses in cancer patients treated with dendritic cell-based immunotherapy are dictated by different vaccination parameters

    Journal: Cancer Immunology, Immunotherapy

    doi: 10.1007/s00262-012-1263-z

    A detailed characterization of KLH-specific antibody responses in individual patients, two examples. Two patients who received 9 vaccinations of KLH-loaded dendritic cells over a period of 18 months are characterized in detail for KLH-specific antibody responses. Each vaccination is indicated by a black arrow . One patient had a robust humoral response, the kinetics of the KLH-specific IgG, IgA and IgM responses are shown in ( a ). In b, the IgG immune response is subdivided into the four IgG subclasses, demonstrating that IgG1 predominantly contributes to the KLH-specific immune response in this patient. The second patient received vaccinations with DC not loaded with KLH. In this patient, KLH-specific antibody responses were completely absent ( c , d )
    Figure Legend Snippet: A detailed characterization of KLH-specific antibody responses in individual patients, two examples. Two patients who received 9 vaccinations of KLH-loaded dendritic cells over a period of 18 months are characterized in detail for KLH-specific antibody responses. Each vaccination is indicated by a black arrow . One patient had a robust humoral response, the kinetics of the KLH-specific IgG, IgA and IgM responses are shown in ( a ). In b, the IgG immune response is subdivided into the four IgG subclasses, demonstrating that IgG1 predominantly contributes to the KLH-specific immune response in this patient. The second patient received vaccinations with DC not loaded with KLH. In this patient, KLH-specific antibody responses were completely absent ( c , d )

    Techniques Used:

    Variations in vaccination parameters induce different humoral anti-KLH responses . In total, 128 melanoma patients were exposed to KLH by 3 bi-weekly vaccinations containing KLH-loaded DC. None of the 35 patients tested (protocols 4 and 5) had KLH-specific antibodies prior to vaccination ( a ). KLH-specific Ab responses compared between a patients treated with or without daclizumab prior to the KLH exposure; b patients vaccinated with immature or mature KLH-loaded DC; c peptide-loaded or mRNA-transfected KLH-loaded DC; d intranodal or intravenous/intradermal routes of administration and e patients with locoregional metastatic disease or distant metastatic disease at inclusion. Mean levels of KLH-specific IgG ( white bars ), IgA ( gray bars ) and IgM ( black bars ) antibodies are shown in mg/L. Error bars indicate standard error of the mean; Nd not detected
    Figure Legend Snippet: Variations in vaccination parameters induce different humoral anti-KLH responses . In total, 128 melanoma patients were exposed to KLH by 3 bi-weekly vaccinations containing KLH-loaded DC. None of the 35 patients tested (protocols 4 and 5) had KLH-specific antibodies prior to vaccination ( a ). KLH-specific Ab responses compared between a patients treated with or without daclizumab prior to the KLH exposure; b patients vaccinated with immature or mature KLH-loaded DC; c peptide-loaded or mRNA-transfected KLH-loaded DC; d intranodal or intravenous/intradermal routes of administration and e patients with locoregional metastatic disease or distant metastatic disease at inclusion. Mean levels of KLH-specific IgG ( white bars ), IgA ( gray bars ) and IgM ( black bars ) antibodies are shown in mg/L. Error bars indicate standard error of the mean; Nd not detected

    Techniques Used: Transfection

    Serial dilution linearity of the anti-KLH ELISA . Serum samples of patients exposed to KLH were serially diluted with assay buffer and measured in quadruplicate. The results for linearity of the ELISA for the anti-KLH isotypes IgG ( a ) IgA ( b ) and IgM ( c ) are shown. Calculated concentrations are based on stock-concentration and dilution factor. Slopes and coefficients of determination (R 2 ) are indicated in each panel. The line of identity is indicated by the dashed line
    Figure Legend Snippet: Serial dilution linearity of the anti-KLH ELISA . Serum samples of patients exposed to KLH were serially diluted with assay buffer and measured in quadruplicate. The results for linearity of the ELISA for the anti-KLH isotypes IgG ( a ) IgA ( b ) and IgM ( c ) are shown. Calculated concentrations are based on stock-concentration and dilution factor. Slopes and coefficients of determination (R 2 ) are indicated in each panel. The line of identity is indicated by the dashed line

    Techniques Used: Serial Dilution, Enzyme-linked Immunosorbent Assay, Concentration Assay

    21) Product Images from "Idiopathic multicentric Castleman's disease: a clinicopathologic study in comparison with IgG4-related disease"

    Article Title: Idiopathic multicentric Castleman's disease: a clinicopathologic study in comparison with IgG4-related disease

    Journal: Oncotarget

    doi: 10.18632/oncotarget.24068

    Histopathologic features of lymph nodes involved in IgG4-RD (A) The lymphoid follicle is hyperplastic. (B) Blood vessels in the germinal center are hyalinized. (C) Plasmacytic infiltration is associated with lymphocytes in between. (D) Occasional eosinophils are also observed. (E) Many IgG4-positive plasma cells are diffusely present (IgG4 immunostaining). (F) The IgG4/IgG-positive plasma cell ratio is increased to > 40% (IgG immunostaining). Images E and F were taken at almost the same fields.
    Figure Legend Snippet: Histopathologic features of lymph nodes involved in IgG4-RD (A) The lymphoid follicle is hyperplastic. (B) Blood vessels in the germinal center are hyalinized. (C) Plasmacytic infiltration is associated with lymphocytes in between. (D) Occasional eosinophils are also observed. (E) Many IgG4-positive plasma cells are diffusely present (IgG4 immunostaining). (F) The IgG4/IgG-positive plasma cell ratio is increased to > 40% (IgG immunostaining). Images E and F were taken at almost the same fields.

    Techniques Used: Immunostaining

    Histopathologic features of lungs involved in iMCD (A) A dense inflammatory infiltrate is observed around the bronchiole. (B) Many plasma cells are arranged in sheets with almost no intervening lymphocytes. (C) Amyloid-like hyalinizing fibrosis is broadly observed. (D) The lymphoid follicle contains a regressed germinal center with hyalinized blood vessels. (E) Many IgG4-positive plasma cells are identified (IgG4 immunostaining). (F) The ratio of IgG4/IgG-positive plasma cells are approximately 40% (IgG immunostaining). Images E and F were taken at almost the same fields.
    Figure Legend Snippet: Histopathologic features of lungs involved in iMCD (A) A dense inflammatory infiltrate is observed around the bronchiole. (B) Many plasma cells are arranged in sheets with almost no intervening lymphocytes. (C) Amyloid-like hyalinizing fibrosis is broadly observed. (D) The lymphoid follicle contains a regressed germinal center with hyalinized blood vessels. (E) Many IgG4-positive plasma cells are identified (IgG4 immunostaining). (F) The ratio of IgG4/IgG-positive plasma cells are approximately 40% (IgG immunostaining). Images E and F were taken at almost the same fields.

    Techniques Used: Immunostaining

    Histopathologic features of lungs involved in IgG4-RD (A) The inflammatory process infiltrates the alveolar interstitium. (B) An organizing pneumonia pattern is focally seen. (C) Although many plasma cells are observed, intervening lymphocytes are also present. (D) Occasional eosinophils are identified. (E and F) The absolute number of IgG4-positive plasma cells and ratio of IgG4/IgG-positive plasma cells are both increased (E, IgG4 immunostaining; F, IgG immunostaining). Images E and F were taken at almost the same fields.
    Figure Legend Snippet: Histopathologic features of lungs involved in IgG4-RD (A) The inflammatory process infiltrates the alveolar interstitium. (B) An organizing pneumonia pattern is focally seen. (C) Although many plasma cells are observed, intervening lymphocytes are also present. (D) Occasional eosinophils are identified. (E and F) The absolute number of IgG4-positive plasma cells and ratio of IgG4/IgG-positive plasma cells are both increased (E, IgG4 immunostaining; F, IgG immunostaining). Images E and F were taken at almost the same fields.

    Techniques Used: Immunostaining

    Histopathologic features of lymph nodes involved in iMCD (A) The lymphoid follicle is hyperplastic. (B) The mantle zone is expanded with a laminated appearance, while germinal centers are regressed. (C) Follicular dendritic cells are prominent with a small number of lymphocytes in the germinal center. (D) The interfollicular area shows sheet-like plasmacytosis. (E) Many IgG4-positive plasma cells are observed (IgG4 immunostaining). (F) IgG-positive plasma cells also increase in number with an IgG4/IgG-positive plasma cell ratio
    Figure Legend Snippet: Histopathologic features of lymph nodes involved in iMCD (A) The lymphoid follicle is hyperplastic. (B) The mantle zone is expanded with a laminated appearance, while germinal centers are regressed. (C) Follicular dendritic cells are prominent with a small number of lymphocytes in the germinal center. (D) The interfollicular area shows sheet-like plasmacytosis. (E) Many IgG4-positive plasma cells are observed (IgG4 immunostaining). (F) IgG-positive plasma cells also increase in number with an IgG4/IgG-positive plasma cell ratio

    Techniques Used: Immunostaining

    22) Product Images from "Serological Monitoring of Progression of Alveolar Echinococcosis with Multiorgan Involvement by Use of Recombinant Em18 "

    Article Title: Serological Monitoring of Progression of Alveolar Echinococcosis with Multiorgan Involvement by Use of Recombinant Em18

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01111-09

    Antibody responses to recombinant Em18 over the course of follow-up of the two AE cases. Four different secondary antibodies (anti-human IgG [Fab], anti-human IgG [H+L], anti-human IgG1, and anti-human IgG4) and recombinant protein G (Pro G) were
    Figure Legend Snippet: Antibody responses to recombinant Em18 over the course of follow-up of the two AE cases. Four different secondary antibodies (anti-human IgG [Fab], anti-human IgG [H+L], anti-human IgG1, and anti-human IgG4) and recombinant protein G (Pro G) were

    Techniques Used: Recombinant

    23) Product Images from "IgG4-Related Disease and Hypertrophic Pachymeningitis"

    Article Title: IgG4-Related Disease and Hypertrophic Pachymeningitis

    Journal: Medicine

    doi: 10.1097/MD.0b013e31829cce35

    Histopathologic findings in pachymeningitis caused by granulomatosis with polyangiitis (GPA). A. (Case 6) GPA—multinucleated giant cells seen in a meningeal biopsy. B. GPA–microabscess surrounded by histiocytes. C. GPA—storiform fibrosis is present in this example. D. A case of GPA with markedly elevated numbers of IgG4+ plasma cells. [This figure can be viewed in color online at http://www.md-journal.com .]
    Figure Legend Snippet: Histopathologic findings in pachymeningitis caused by granulomatosis with polyangiitis (GPA). A. (Case 6) GPA—multinucleated giant cells seen in a meningeal biopsy. B. GPA–microabscess surrounded by histiocytes. C. GPA—storiform fibrosis is present in this example. D. A case of GPA with markedly elevated numbers of IgG4+ plasma cells. [This figure can be viewed in color online at http://www.md-journal.com .]

    Techniques Used:

    Histopathologic findings in IgG4-related pachymeningitis. A. (Case 2) The tumoral lesion is composed predominantly of fibrosis, lymphocytes, and plasma cells. B. (Case 2) Microgranuloma composed of epithelioid histiocytes. C. (Case 3) An IgG4 immunoperoxidase stain shows numerous IgG4+ plasma cells throughout the lesion. D. Higher power image shows storiform-type fibrosis. [This figure can be viewed in color online at http://www.md-journal.com .]
    Figure Legend Snippet: Histopathologic findings in IgG4-related pachymeningitis. A. (Case 2) The tumoral lesion is composed predominantly of fibrosis, lymphocytes, and plasma cells. B. (Case 2) Microgranuloma composed of epithelioid histiocytes. C. (Case 3) An IgG4 immunoperoxidase stain shows numerous IgG4+ plasma cells throughout the lesion. D. Higher power image shows storiform-type fibrosis. [This figure can be viewed in color online at http://www.md-journal.com .]

    Techniques Used: Staining

    MRI findings of non-IgG4-related pachymeningitis. A. Rheumatoid arthritis-associated pachy- and leptomeningitis: coronal T1 postgadolinium MRI showing left frontal and falcine pachymeningeal enhancement with prominent leptomeningeal enhancement in the left frontal lobe and bilateral medial frontal lobes. B. (Case 11) Lymphoma: coronal T1 postgadolinium MRI shows a multilobulated extraaxial 3.4 × 5.7 × 6.0 cm homogenously enhancing mass arising from the tentorium and causing mass effect on the left temp/parieto-occipital junction and left cerebellum. C and D. (Case 10) Neurosarcoidosis: axial T1 MRIs before (C) and after (D) administration of gadolinium MRI performed 1 year after initial symptoms show supra-sellar, cavernous sinus, and clival enhancement.
    Figure Legend Snippet: MRI findings of non-IgG4-related pachymeningitis. A. Rheumatoid arthritis-associated pachy- and leptomeningitis: coronal T1 postgadolinium MRI showing left frontal and falcine pachymeningeal enhancement with prominent leptomeningeal enhancement in the left frontal lobe and bilateral medial frontal lobes. B. (Case 11) Lymphoma: coronal T1 postgadolinium MRI shows a multilobulated extraaxial 3.4 × 5.7 × 6.0 cm homogenously enhancing mass arising from the tentorium and causing mass effect on the left temp/parieto-occipital junction and left cerebellum. C and D. (Case 10) Neurosarcoidosis: axial T1 MRIs before (C) and after (D) administration of gadolinium MRI performed 1 year after initial symptoms show supra-sellar, cavernous sinus, and clival enhancement.

    Techniques Used: Magnetic Resonance Imaging

    MRI findings of IgG4-related pachymeningitis. A. (Case 1) Coronal T1 postgadolinium image with lobulated expansile lesions of the falx and parafalcine dura with areas of mass effect on the brain. B. (Case 3) Coronal T1 postgadolinium image shows left cerebral convexity lepto- and pachymeningitis. C and D. (Case 2) Axial T1 pre- and postgadolinium images disclosing right-sided pachymeningitis involving the tentorium and posterior fossa with areas of right cerebellar leptomeningeal enhancement adjacent to right mastoid air cells enhancement.
    Figure Legend Snippet: MRI findings of IgG4-related pachymeningitis. A. (Case 1) Coronal T1 postgadolinium image with lobulated expansile lesions of the falx and parafalcine dura with areas of mass effect on the brain. B. (Case 3) Coronal T1 postgadolinium image shows left cerebral convexity lepto- and pachymeningitis. C and D. (Case 2) Axial T1 pre- and postgadolinium images disclosing right-sided pachymeningitis involving the tentorium and posterior fossa with areas of right cerebellar leptomeningeal enhancement adjacent to right mastoid air cells enhancement.

    Techniques Used: Magnetic Resonance Imaging

    24) Product Images from "Component-resolved analysis of IgA, IgE, and IgG4 during egg OIT identifies markers associated with sustained unresponsiveness"

    Article Title: Component-resolved analysis of IgA, IgE, and IgG4 during egg OIT identifies markers associated with sustained unresponsiveness

    Journal: Allergy

    doi: 10.1111/all.12895

    Trends in endpoint-to-baseline log ratios between responders and non-responders. Graphs show logarithmic mean and SEM of endpoint-to-baseline ratios for serologic parameters with significant differences between responders and non-responders. Relative increases in the IgG4-EW (A), IgG4-OVM (B), IgA-EW (C), IgA2-EW (D), and the sum of IgG4-EW and IgA-EW (E) are depicted.
    Figure Legend Snippet: Trends in endpoint-to-baseline log ratios between responders and non-responders. Graphs show logarithmic mean and SEM of endpoint-to-baseline ratios for serologic parameters with significant differences between responders and non-responders. Relative increases in the IgG4-EW (A), IgG4-OVM (B), IgA-EW (C), IgA2-EW (D), and the sum of IgG4-EW and IgA-EW (E) are depicted.

    Techniques Used:

    Immunoglobulin trends for antigen-specific IgE, IgG4, IgA, IgA1, and IgA2 among groups. Responder status defined as passing a DBPCFC after at least 4 weeks off therapy at 22, 36 or 48 months. Graphs show logarithmic mean and SEM for each immunoglobulin measurement. Horizontal axis depicts time in months during the clinical trial.
    Figure Legend Snippet: Immunoglobulin trends for antigen-specific IgE, IgG4, IgA, IgA1, and IgA2 among groups. Responder status defined as passing a DBPCFC after at least 4 weeks off therapy at 22, 36 or 48 months. Graphs show logarithmic mean and SEM for each immunoglobulin measurement. Horizontal axis depicts time in months during the clinical trial.

    Techniques Used:

    Log ratios of IgA, IgA1, IgA2 and IgG4 to IgE for EW, OVA and OVM in responders versus non-responders. Graphs show logarithmic mean and SE for ratios with significant differences between responders and non-responders. Ratios of IgG4:IgE, IgA:IgE, IgA2:IgE to EW and IgA:IgE to OVA have significant time by responder group interactions.
    Figure Legend Snippet: Log ratios of IgA, IgA1, IgA2 and IgG4 to IgE for EW, OVA and OVM in responders versus non-responders. Graphs show logarithmic mean and SE for ratios with significant differences between responders and non-responders. Ratios of IgG4:IgE, IgA:IgE, IgA2:IgE to EW and IgA:IgE to OVA have significant time by responder group interactions.

    Techniques Used:

    25) Product Images from "IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens"

    Article Title: IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens

    Journal: The Journal of Allergy and Clinical Immunology

    doi: 10.1016/j.jaci.2015.01.012

    IgG 4 /IgE ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test.
    Figure Legend Snippet: IgG 4 /IgE ratios to peanut and peanut allergens in children with PA (n = 65) and PS children (n = 27). Values are presented as medians and interquartile ranges. P values refer to the comparison between children with PA and PS children using the Mann-Whitney U test.

    Techniques Used: MANN-WHITNEY

    Inhibition of peanut-induced activation of mast cells sensitized with plasma from a patient with PA in the presence of mock- or IgG 4 -depleted plasma samples from peanut-sensitized tolerant patients (median inhibition, 75% vs 30%, respectively; P = .007, n = 12). % Inhibition = (% CD63 + cells sensitized with plasma from a patient with PA − % CD63 + cells sensitized with plasma from a patient with PA in the presence of test plasma)/% CD63 + cells sensitized with plasma from a patient with PA. The P value refers to the comparison between IgG 4 - and mock-depleted paired samples by using the Wilcoxon signed-rank test. ** P
    Figure Legend Snippet: Inhibition of peanut-induced activation of mast cells sensitized with plasma from a patient with PA in the presence of mock- or IgG 4 -depleted plasma samples from peanut-sensitized tolerant patients (median inhibition, 75% vs 30%, respectively; P = .007, n = 12). % Inhibition = (% CD63 + cells sensitized with plasma from a patient with PA − % CD63 + cells sensitized with plasma from a patient with PA in the presence of test plasma)/% CD63 + cells sensitized with plasma from a patient with PA. The P value refers to the comparison between IgG 4 - and mock-depleted paired samples by using the Wilcoxon signed-rank test. ** P

    Techniques Used: Inhibition, Activation Assay

    26) Product Images from "Prevalence of IgG Autoantibodies against GD3 Ganglioside in Acute Zika Virus Infection"

    Article Title: Prevalence of IgG Autoantibodies against GD3 Ganglioside in Acute Zika Virus Infection

    Journal: Frontiers in Medicine

    doi: 10.3389/fmed.2018.00025

    Autoantibody levels to gangliosides in Zika-infected patients and healthy control individuals. ELISA plates coated with the indicated gangliosides at 20 µg/ml in ethanol, followed by evaporation, were incubated with 1:100 dilution of sera from Zika patients or control healthy individuals and developed with anti-total IgG or anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 13) and healthy individual ( n = 17) for recognition of (A,C) IgG class autoantibodies and (B,D) IgG subclasses (IgG1, IgG2, IgG3, and IgG4 isotypes), against (A,B) total gangliosides (from bovine brain extract) or (C,D) purified ganglioside GD3. Each data point is the mean of triplicated determinations. Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.01).
    Figure Legend Snippet: Autoantibody levels to gangliosides in Zika-infected patients and healthy control individuals. ELISA plates coated with the indicated gangliosides at 20 µg/ml in ethanol, followed by evaporation, were incubated with 1:100 dilution of sera from Zika patients or control healthy individuals and developed with anti-total IgG or anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 13) and healthy individual ( n = 17) for recognition of (A,C) IgG class autoantibodies and (B,D) IgG subclasses (IgG1, IgG2, IgG3, and IgG4 isotypes), against (A,B) total gangliosides (from bovine brain extract) or (C,D) purified ganglioside GD3. Each data point is the mean of triplicated determinations. Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.01).

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Evaporation, Incubation, Purification

    Increased levels of IgG4 autoantibody against to GD3 ganglioside in Zika virus infection during pregnancy. ELISA plates coated with GD3 ganglioside (20 µg/ml) were incubated with 1:100 dilution of sera from pregnant patients with Zika infection or control healthy pregnant individuals. The sera was obtained after pregnancy, and the reaction was developed with (A) anti-total IgG or (B) anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 12) and healthy individual ( n = 8). Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05).
    Figure Legend Snippet: Increased levels of IgG4 autoantibody against to GD3 ganglioside in Zika virus infection during pregnancy. ELISA plates coated with GD3 ganglioside (20 µg/ml) were incubated with 1:100 dilution of sera from pregnant patients with Zika infection or control healthy pregnant individuals. The sera was obtained after pregnancy, and the reaction was developed with (A) anti-total IgG or (B) anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 12) and healthy individual ( n = 8). Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05).

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Incubation

    27) Product Images from "Immunization with Wuchereria bancrofti Glutathione-S-transferase Elicits a Mixed Th1/Th2 Type of Protective Immune Response Against Filarial Infection in Mastomys"

    Article Title: Immunization with Wuchereria bancrofti Glutathione-S-transferase Elicits a Mixed Th1/Th2 Type of Protective Immune Response Against Filarial Infection in Mastomys

    Journal: Indian Journal of Clinical Biochemistry

    doi: 10.1007/s12291-016-0556-y

    Measurement of anti-WbGST IgG antibody isotypes in the mastomys immunized with rWbGST. The levels of anti-WbGST IgG antibody isotypes in the sera of mastomys immunized with rWbGST were measured by ELISA. Each data point represents Mean ± SD;
    Figure Legend Snippet: Measurement of anti-WbGST IgG antibody isotypes in the mastomys immunized with rWbGST. The levels of anti-WbGST IgG antibody isotypes in the sera of mastomys immunized with rWbGST were measured by ELISA. Each data point represents Mean ± SD;

    Techniques Used: Enzyme-linked Immunosorbent Assay

    28) Product Images from "Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure"

    Article Title: Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure

    Journal: BMC Medicine

    doi: 10.1186/s12916-019-1277-x

    Relationship between age and immunity. Children in RTS,S vaccine group from Manhiça (black box plots) and Ilha Josina cohorts (gray box plots) were categorized into younger (12 to 24 months; Manhiça n = 11 and Ilha Josina n = 23, respectively) and older (24 to 60 months; Manhiça n = 39 and Ilha Josina n = 26, respectively) age groups. Sera collected after vaccination (month 3, M3) were tested for C1q-fixation to CSP and NANP ( a ) and IgG-reactivity to blood-stage antigens MSP2 and AMA1 ( b ). Samples were tested in duplicate, and the mean value was used to generate box plots for samples stratified by age group. Top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between unpaired samples was compared using Mann-Whitney U test
    Figure Legend Snippet: Relationship between age and immunity. Children in RTS,S vaccine group from Manhiça (black box plots) and Ilha Josina cohorts (gray box plots) were categorized into younger (12 to 24 months; Manhiça n = 11 and Ilha Josina n = 23, respectively) and older (24 to 60 months; Manhiça n = 39 and Ilha Josina n = 26, respectively) age groups. Sera collected after vaccination (month 3, M3) were tested for C1q-fixation to CSP and NANP ( a ) and IgG-reactivity to blood-stage antigens MSP2 and AMA1 ( b ). Samples were tested in duplicate, and the mean value was used to generate box plots for samples stratified by age group. Top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between unpaired samples was compared using Mann-Whitney U test

    Techniques Used: MANN-WHITNEY

    RTS,S vaccine-induced immunity declines over time. A random selection of children vaccinated with RTS,S (Manhiça cohort, n = 30) was tested for C1q-fixation ( a ), IgG/IgM ( b ), and IgG subclasses ( c ) to CSP at months 3, 8.5, 21, 33, 45, and 6. Note that due to low reactivity, C1q-fixation was re-tested at a higher dilution of 1/110, in addition to 1/250, to confirm results. Samples were tested in duplicate, and the median and 95% CI of the median from each time point group are shown by the symbol and shaded area, respectively
    Figure Legend Snippet: RTS,S vaccine-induced immunity declines over time. A random selection of children vaccinated with RTS,S (Manhiça cohort, n = 30) was tested for C1q-fixation ( a ), IgG/IgM ( b ), and IgG subclasses ( c ) to CSP at months 3, 8.5, 21, 33, 45, and 6. Note that due to low reactivity, C1q-fixation was re-tested at a higher dilution of 1/110, in addition to 1/250, to confirm results. Samples were tested in duplicate, and the median and 95% CI of the median from each time point group are shown by the symbol and shaded area, respectively

    Techniques Used: Selection

    RTS,S vaccine-induced IgG and IgM antibodies to CSP. Children in RTS,S and comparator vaccine groups from Manhiça (black box plots; N = 50 and N = 25, respectively) and Ilha Josina cohorts (gray box plots; N = 49 and N = 24, respectively) were tested for IgG ( a ), IgG subclasses ( b ), and IgM ( c ) to CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate (note that only M3 was tested in b ), and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff value (dashed lines), and the percentage of individuals above this threshold are shown. Reactivity between paired samples and unpaired samples were compared using Wilcoxon matched-pairs signed-rank test and Mann-Whitney U test, respectively
    Figure Legend Snippet: RTS,S vaccine-induced IgG and IgM antibodies to CSP. Children in RTS,S and comparator vaccine groups from Manhiça (black box plots; N = 50 and N = 25, respectively) and Ilha Josina cohorts (gray box plots; N = 49 and N = 24, respectively) were tested for IgG ( a ), IgG subclasses ( b ), and IgM ( c ) to CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate (note that only M3 was tested in b ), and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff value (dashed lines), and the percentage of individuals above this threshold are shown. Reactivity between paired samples and unpaired samples were compared using Wilcoxon matched-pairs signed-rank test and Mann-Whitney U test, respectively

    Techniques Used: MANN-WHITNEY

    High variability among RTS,S vaccine-induced IgG targeting the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, 3 M) were tested for IgG to NANP and CT and C1q-fixation to CSP, and the values were used for the following analysis. a Heat map of children arranged in descending (top to bottom) order of C1q-fixation (left), corresponding IgG to NANP and CT (middle), and for comparison IgM to NANP and CT (right). b Variability between epitope specificity was quantified by calculating the ratio of NANP-to-CT IgG. Children with low variability were considered to have equal reactivity to NANP and CT (ratio between 0.75 and 1.25 shown in white), and children exceeding this range were considered to have a NANP- or CT-skewed response (ratio > 1.25 shown in purple and ratio
    Figure Legend Snippet: High variability among RTS,S vaccine-induced IgG targeting the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, 3 M) were tested for IgG to NANP and CT and C1q-fixation to CSP, and the values were used for the following analysis. a Heat map of children arranged in descending (top to bottom) order of C1q-fixation (left), corresponding IgG to NANP and CT (middle), and for comparison IgM to NANP and CT (right). b Variability between epitope specificity was quantified by calculating the ratio of NANP-to-CT IgG. Children with low variability were considered to have equal reactivity to NANP and CT (ratio between 0.75 and 1.25 shown in white), and children exceeding this range were considered to have a NANP- or CT-skewed response (ratio > 1.25 shown in purple and ratio

    Techniques Used:

    Functional complement-fixing antibodies target the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça (black box plots; N = 50) and Ilha Josina cohorts (gray box plots; N = 49) were tested for IgG ( a ) and IgM ( b ) to NANP and CT regions of CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate, and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between paired samples was compared using Wilcoxon matched-pairs signed-rank test. c , d Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, M3) were tested for C1q-fixation to CSP, NANP, and CT, and the values were plotted compared to IgG reactivity (c) and C1q fixation to CSP, NANP, and CT were correlated (d)
    Figure Legend Snippet: Functional complement-fixing antibodies target the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça (black box plots; N = 50) and Ilha Josina cohorts (gray box plots; N = 49) were tested for IgG ( a ) and IgM ( b ) to NANP and CT regions of CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate, and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between paired samples was compared using Wilcoxon matched-pairs signed-rank test. c , d Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, M3) were tested for C1q-fixation to CSP, NANP, and CT, and the values were plotted compared to IgG reactivity (c) and C1q fixation to CSP, NANP, and CT were correlated (d)

    Techniques Used: Functional Assay

    29) Product Images from "Erdheim-Chester Disease as a Mimic of IgG4-Related Disease"

    Article Title: Erdheim-Chester Disease as a Mimic of IgG4-Related Disease

    Journal: Medicine

    doi: 10.1097/MD.0000000000003625

    A, Contrast-enhanced computed tomography (CT) of the abdomen (coronal view, arterial phase) shows bilateral inhomogeneous masses occupying most of the abdomen and partially distorting the renal parenchyma; the left kidney (contrast-enhanced) is completely enveloped by the mass, whereas in this scan, only the upper pole of the right kidney (arrow) is visible within the right-sided mass. B, Sagittal view passing through the left kidney: the mass is all around the kidney and obstructs the ureter, which has an indwelling stent pointing backwards (arrow); of note, both masses reached the anterior abdominal wall and were easily palpable. C, Late “urographic” scan: both kidneys are contrast-enhanced; the axial diameters of the perirenal masses were 22 × 19 cm (right side) and 23 × 14 cm (left side). D, Medium-power histopathological view of the Erdheim-Chester lesion characterized by many foamy histiocytes (arrow) and a chronic inflammatory infiltrate (asterisk) which is mainly made of lymphocytes and plasma cells. E, CD138 immunohistochemical antibody decorates the plasma cell membranes (arrow). F, Immunohistochemistry with an anti-IgG4 antibody is positive in a large number of plasma cells (arrow). Stainings: A, hematoxylin-eosin; B and C, mildly counterstained with hematoxylin. Original magnifications: A–C, ×20 (scale bar is 100 μm).
    Figure Legend Snippet: A, Contrast-enhanced computed tomography (CT) of the abdomen (coronal view, arterial phase) shows bilateral inhomogeneous masses occupying most of the abdomen and partially distorting the renal parenchyma; the left kidney (contrast-enhanced) is completely enveloped by the mass, whereas in this scan, only the upper pole of the right kidney (arrow) is visible within the right-sided mass. B, Sagittal view passing through the left kidney: the mass is all around the kidney and obstructs the ureter, which has an indwelling stent pointing backwards (arrow); of note, both masses reached the anterior abdominal wall and were easily palpable. C, Late “urographic” scan: both kidneys are contrast-enhanced; the axial diameters of the perirenal masses were 22 × 19 cm (right side) and 23 × 14 cm (left side). D, Medium-power histopathological view of the Erdheim-Chester lesion characterized by many foamy histiocytes (arrow) and a chronic inflammatory infiltrate (asterisk) which is mainly made of lymphocytes and plasma cells. E, CD138 immunohistochemical antibody decorates the plasma cell membranes (arrow). F, Immunohistochemistry with an anti-IgG4 antibody is positive in a large number of plasma cells (arrow). Stainings: A, hematoxylin-eosin; B and C, mildly counterstained with hematoxylin. Original magnifications: A–C, ×20 (scale bar is 100 μm).

    Techniques Used: Computed Tomography, Immunohistochemistry

    30) Product Images from "Prevalence of IgG Autoantibodies against GD3 Ganglioside in Acute Zika Virus Infection"

    Article Title: Prevalence of IgG Autoantibodies against GD3 Ganglioside in Acute Zika Virus Infection

    Journal: Frontiers in Medicine

    doi: 10.3389/fmed.2018.00025

    Autoantibody levels to gangliosides in Zika-infected patients and healthy control individuals. ELISA plates coated with the indicated gangliosides at 20 µg/ml in ethanol, followed by evaporation, were incubated with 1:100 dilution of sera from Zika patients or control healthy individuals and developed with anti-total IgG or anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 13) and healthy individual ( n = 17) for recognition of (A,C) IgG class autoantibodies and (B,D) IgG subclasses (IgG1, IgG2, IgG3, and IgG4 isotypes), against (A,B) total gangliosides (from bovine brain extract) or (C,D) purified ganglioside GD3. Each data point is the mean of triplicated determinations. Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.01).
    Figure Legend Snippet: Autoantibody levels to gangliosides in Zika-infected patients and healthy control individuals. ELISA plates coated with the indicated gangliosides at 20 µg/ml in ethanol, followed by evaporation, were incubated with 1:100 dilution of sera from Zika patients or control healthy individuals and developed with anti-total IgG or anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 13) and healthy individual ( n = 17) for recognition of (A,C) IgG class autoantibodies and (B,D) IgG subclasses (IgG1, IgG2, IgG3, and IgG4 isotypes), against (A,B) total gangliosides (from bovine brain extract) or (C,D) purified ganglioside GD3. Each data point is the mean of triplicated determinations. Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.01).

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Evaporation, Incubation, Purification

    Increased levels of IgG4 autoantibody against to GD3 ganglioside in Zika virus infection during pregnancy. ELISA plates coated with GD3 ganglioside (20 µg/ml) were incubated with 1:100 dilution of sera from pregnant patients with Zika infection or control healthy pregnant individuals. The sera was obtained after pregnancy, and the reaction was developed with (A) anti-total IgG or (B) anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 12) and healthy individual ( n = 8). Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05).
    Figure Legend Snippet: Increased levels of IgG4 autoantibody against to GD3 ganglioside in Zika virus infection during pregnancy. ELISA plates coated with GD3 ganglioside (20 µg/ml) were incubated with 1:100 dilution of sera from pregnant patients with Zika infection or control healthy pregnant individuals. The sera was obtained after pregnancy, and the reaction was developed with (A) anti-total IgG or (B) anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient ( n = 12) and healthy individual ( n = 8). Means of data points for each individual ± SE are shown. Differences between groups are significant *( p ≤ 0.05).

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Incubation

    31) Product Images from "Diverse IgG Subclass Responses to Adeno-associated Virus Infection and Vector Administration"

    Article Title: Diverse IgG Subclass Responses to Adeno-associated Virus Infection and Vector Administration

    Journal: Journal of medical virology

    doi: 10.1002/jmv.21360

    Distribution of IgG subclass responses to AAV capsid in normal human subjects The percent of total capsid-specific IgG is shown for each IgG subclass for 32 normal human subjects.
    Figure Legend Snippet: Distribution of IgG subclass responses to AAV capsid in normal human subjects The percent of total capsid-specific IgG is shown for each IgG subclass for 32 normal human subjects.

    Techniques Used:

    32) Product Images from "Basophil Reactivity, Wheal Size and Immunoglobulin Levels Distinguish Degree of Cow's Milk Tolerance"

    Article Title: Basophil Reactivity, Wheal Size and Immunoglobulin Levels Distinguish Degree of Cow's Milk Tolerance

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2012.06.003

    Ratio of casein-specific IgE to IgG 4 ratio by five levels of challenge outcome. With the three central groups pooled, post-hoc analysis showed a significant difference between those who reacted to baked-milk and those who tolerated it (p
    Figure Legend Snippet: Ratio of casein-specific IgE to IgG 4 ratio by five levels of challenge outcome. With the three central groups pooled, post-hoc analysis showed a significant difference between those who reacted to baked-milk and those who tolerated it (p

    Techniques Used:

    Casein-specific IgG 4 by five levels of challenge outcome.
    Figure Legend Snippet: Casein-specific IgG 4 by five levels of challenge outcome.

    Techniques Used:

    33) Product Images from "Association between IgG4 Autoantibody and Complement Abnormalities in Systemic Lupus Erythematosus"

    Article Title: Association between IgG4 Autoantibody and Complement Abnormalities in Systemic Lupus Erythematosus

    Journal: Mediators of Inflammation

    doi: 10.1155/2016/2196986

    Comparison of serum levels of IgG4-specific IgM-RF in various groups. Detection of IgG4-specific IgM-RF in the serum of normals and SLE and RA patients was conducted by ELISA as described in Section 2 (normal = 41, SLE = 72, and RA = 67; ∗∗∗ P
    Figure Legend Snippet: Comparison of serum levels of IgG4-specific IgM-RF in various groups. Detection of IgG4-specific IgM-RF in the serum of normals and SLE and RA patients was conducted by ELISA as described in Section 2 (normal = 41, SLE = 72, and RA = 67; ∗∗∗ P

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Levels of IgG subclass, C1q, and C3 deposition in lupus nephritis. (a) IgG subclass, C1q, and C3 deposition in LN renal tissue were detected by direct immunofluorescence staining as described in Section 2 . (b) The correlations between the ratio of deposition score for IgG4/(IgG1 + IgG2 + IgG3 + IgG4) and the score for C1q and C3 deposition were analyzed using Spearman's rank correlation coefficient. The P value was considered statistically significant if it was less than 0.05 ( n = 141, including 46 newly diagnosed LN patients and 65 LN patients treated with prednisone plus cyclophosphamide or azathioprine or hydroxychloroquine or mycophenolate mofetil).
    Figure Legend Snippet: Levels of IgG subclass, C1q, and C3 deposition in lupus nephritis. (a) IgG subclass, C1q, and C3 deposition in LN renal tissue were detected by direct immunofluorescence staining as described in Section 2 . (b) The correlations between the ratio of deposition score for IgG4/(IgG1 + IgG2 + IgG3 + IgG4) and the score for C1q and C3 deposition were analyzed using Spearman's rank correlation coefficient. The P value was considered statistically significant if it was less than 0.05 ( n = 141, including 46 newly diagnosed LN patients and 65 LN patients treated with prednisone plus cyclophosphamide or azathioprine or hydroxychloroquine or mycophenolate mofetil).

    Techniques Used: Immunofluorescence, Staining

    Comparison of serum levels of anti-nuclear IgG4 in various groups. Detection of serum levels of anti-nuclear IgG4 in the serum of normals and SLE and RA patients was conducted using a molecular biology kit as described in Section 2 (normal = 41, SLE = 72, and RA = 67; ∗∗ P
    Figure Legend Snippet: Comparison of serum levels of anti-nuclear IgG4 in various groups. Detection of serum levels of anti-nuclear IgG4 in the serum of normals and SLE and RA patients was conducted using a molecular biology kit as described in Section 2 (normal = 41, SLE = 72, and RA = 67; ∗∗ P

    Techniques Used:

    The correlations between serum levels of anti-nuclear IgG4 in SLE patients and clinical parameters. Detection of serum levels of IgG4 and anti-nuclear IgG4 was conducted by ELISA as described in Section 2 . Serum levels of albumin, C3, Scr, SUA, and 24-hour urinary protein were analyzed using a commercial kit as described in Section 2.2 . The correlations between serum levels of anti-nuclear IgG4 and serum levels of C3, albumin, total IgG4, and 24-hour urinary protein were analyzed using Spearman's rank correlation coefficient. The P value was considered statistically significant if it was less than 0.05. ( n = 72, newly diagnosed SLE patients).
    Figure Legend Snippet: The correlations between serum levels of anti-nuclear IgG4 in SLE patients and clinical parameters. Detection of serum levels of IgG4 and anti-nuclear IgG4 was conducted by ELISA as described in Section 2 . Serum levels of albumin, C3, Scr, SUA, and 24-hour urinary protein were analyzed using a commercial kit as described in Section 2.2 . The correlations between serum levels of anti-nuclear IgG4 and serum levels of C3, albumin, total IgG4, and 24-hour urinary protein were analyzed using Spearman's rank correlation coefficient. The P value was considered statistically significant if it was less than 0.05. ( n = 72, newly diagnosed SLE patients).

    Techniques Used: Enzyme-linked Immunosorbent Assay

    34) Product Images from "Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG"

    Article Title: Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0016741

    Specific IgE, IgG 1 and IgG 4 over a 2 year period from 10 patients undergoing VIT. A. Median values of JJAV and Myr p 2a. B. Specific antibody levels.
    Figure Legend Snippet: Specific IgE, IgG 1 and IgG 4 over a 2 year period from 10 patients undergoing VIT. A. Median values of JJAV and Myr p 2a. B. Specific antibody levels.

    Techniques Used:

    35) Product Images from "Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG"

    Article Title: Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0016741

    Specific IgE, IgG 1 and IgG 4 over a 2 year period from 10 patients undergoing VIT. A. Median values of JJAV and Myr p 2a. B. Specific antibody levels.
    Figure Legend Snippet: Specific IgE, IgG 1 and IgG 4 over a 2 year period from 10 patients undergoing VIT. A. Median values of JJAV and Myr p 2a. B. Specific antibody levels.

    Techniques Used:

    36) Product Images from "Subcutaneous Allergen Immunotherapy Restores Human Dendritic Cell Innate Immune Function"

    Article Title: Subcutaneous Allergen Immunotherapy Restores Human Dendritic Cell Innate Immune Function

    Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

    doi: 10.1111/j.1365-2222.2009.03388.x

    Immunoglobulin subtype G4 (IgG4) specific to D. farinae ( Df ) was measured in the serum of seven dust mite allergic subjects before and after a standard subcutaneous allergen immunotherapy protocol (SCIT) and expressed in mgA/L. Serum IgG4 levels pre-SCIT (open circles) increased approximately 10-fold ( P = 0.031) upon reaching maintenance immunotherapy (closed circles). SCIT, subcutaneous allergen immunotherapy; Df , Dermatophagoides farinae .
    Figure Legend Snippet: Immunoglobulin subtype G4 (IgG4) specific to D. farinae ( Df ) was measured in the serum of seven dust mite allergic subjects before and after a standard subcutaneous allergen immunotherapy protocol (SCIT) and expressed in mgA/L. Serum IgG4 levels pre-SCIT (open circles) increased approximately 10-fold ( P = 0.031) upon reaching maintenance immunotherapy (closed circles). SCIT, subcutaneous allergen immunotherapy; Df , Dermatophagoides farinae .

    Techniques Used:

    37) Product Images from "Induction of Ig Somatic Hypermutation and Class Switching in a Human Monoclonal IgM+ IgD+ B Cell Line In Vitro: Definition of the Requirements and Modalities of Hypermutation"

    Article Title: Induction of Ig Somatic Hypermutation and Class Switching in a Human Monoclonal IgM+ IgD+ B Cell Line In Vitro: Definition of the Requirements and Modalities of Hypermutation

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi:

    Stepwise accumulation of somatic point-mutations correlates with class switching from IgM to IgG, IgA, or IgE in CL-01 cells. Depicted is the genealogical tree constructed using related V H DJ H sequences of V H DJ H -C μ , V H DJ H -C δ , V H DJ H -C γ , V H DJ H -C α , and V H DJ H -C ε transcripts from a single culture of CL-01 cells incubated, after BCR engagement, with activated T cells for 14 days. Thin frames depict putative intermediate sequences, and point-mutations are indicated by their codon number and the nature of the base change. Vertical bars depict S mutations, and lollipops depict R mutations.
    Figure Legend Snippet: Stepwise accumulation of somatic point-mutations correlates with class switching from IgM to IgG, IgA, or IgE in CL-01 cells. Depicted is the genealogical tree constructed using related V H DJ H sequences of V H DJ H -C μ , V H DJ H -C δ , V H DJ H -C γ , V H DJ H -C α , and V H DJ H -C ε transcripts from a single culture of CL-01 cells incubated, after BCR engagement, with activated T cells for 14 days. Thin frames depict putative intermediate sequences, and point-mutations are indicated by their codon number and the nature of the base change. Vertical bars depict S mutations, and lollipops depict R mutations.

    Techniques Used: Construct, Incubation

    38) Product Images from "Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure"

    Article Title: Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure

    Journal: BMC Medicine

    doi: 10.1186/s12916-019-1277-x

    Relationship between age and immunity. Children in RTS,S vaccine group from Manhiça (black box plots) and Ilha Josina cohorts (gray box plots) were categorized into younger (12 to 24 months; Manhiça n = 11 and Ilha Josina n = 23, respectively) and older (24 to 60 months; Manhiça n = 39 and Ilha Josina n = 26, respectively) age groups. Sera collected after vaccination (month 3, M3) were tested for C1q-fixation to CSP and NANP ( a ) and IgG-reactivity to blood-stage antigens MSP2 and AMA1 ( b ). Samples were tested in duplicate, and the mean value was used to generate box plots for samples stratified by age group. Top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between unpaired samples was compared using Mann-Whitney U test
    Figure Legend Snippet: Relationship between age and immunity. Children in RTS,S vaccine group from Manhiça (black box plots) and Ilha Josina cohorts (gray box plots) were categorized into younger (12 to 24 months; Manhiça n = 11 and Ilha Josina n = 23, respectively) and older (24 to 60 months; Manhiça n = 39 and Ilha Josina n = 26, respectively) age groups. Sera collected after vaccination (month 3, M3) were tested for C1q-fixation to CSP and NANP ( a ) and IgG-reactivity to blood-stage antigens MSP2 and AMA1 ( b ). Samples were tested in duplicate, and the mean value was used to generate box plots for samples stratified by age group. Top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between unpaired samples was compared using Mann-Whitney U test

    Techniques Used: MANN-WHITNEY

    RTS,S vaccine-induced immunity declines over time. A random selection of children vaccinated with RTS,S (Manhiça cohort, n = 30) was tested for C1q-fixation ( a ), IgG/IgM ( b ), and IgG subclasses ( c ) to CSP at months 3, 8.5, 21, 33, 45, and 6. Note that due to low reactivity, C1q-fixation was re-tested at a higher dilution of 1/110, in addition to 1/250, to confirm results. Samples were tested in duplicate, and the median and 95% CI of the median from each time point group are shown by the symbol and shaded area, respectively
    Figure Legend Snippet: RTS,S vaccine-induced immunity declines over time. A random selection of children vaccinated with RTS,S (Manhiça cohort, n = 30) was tested for C1q-fixation ( a ), IgG/IgM ( b ), and IgG subclasses ( c ) to CSP at months 3, 8.5, 21, 33, 45, and 6. Note that due to low reactivity, C1q-fixation was re-tested at a higher dilution of 1/110, in addition to 1/250, to confirm results. Samples were tested in duplicate, and the median and 95% CI of the median from each time point group are shown by the symbol and shaded area, respectively

    Techniques Used: Selection

    RTS,S vaccine-induced IgG and IgM antibodies to CSP. Children in RTS,S and comparator vaccine groups from Manhiça (black box plots; N = 50 and N = 25, respectively) and Ilha Josina cohorts (gray box plots; N = 49 and N = 24, respectively) were tested for IgG ( a ), IgG subclasses ( b ), and IgM ( c ) to CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate (note that only M3 was tested in b ), and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff value (dashed lines), and the percentage of individuals above this threshold are shown. Reactivity between paired samples and unpaired samples were compared using Wilcoxon matched-pairs signed-rank test and Mann-Whitney U test, respectively
    Figure Legend Snippet: RTS,S vaccine-induced IgG and IgM antibodies to CSP. Children in RTS,S and comparator vaccine groups from Manhiça (black box plots; N = 50 and N = 25, respectively) and Ilha Josina cohorts (gray box plots; N = 49 and N = 24, respectively) were tested for IgG ( a ), IgG subclasses ( b ), and IgM ( c ) to CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate (note that only M3 was tested in b ), and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff value (dashed lines), and the percentage of individuals above this threshold are shown. Reactivity between paired samples and unpaired samples were compared using Wilcoxon matched-pairs signed-rank test and Mann-Whitney U test, respectively

    Techniques Used: MANN-WHITNEY

    High variability among RTS,S vaccine-induced IgG targeting the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, 3 M) were tested for IgG to NANP and CT and C1q-fixation to CSP, and the values were used for the following analysis. a Heat map of children arranged in descending (top to bottom) order of C1q-fixation (left), corresponding IgG to NANP and CT (middle), and for comparison IgM to NANP and CT (right). b Variability between epitope specificity was quantified by calculating the ratio of NANP-to-CT IgG. Children with low variability were considered to have equal reactivity to NANP and CT (ratio between 0.75 and 1.25 shown in white), and children exceeding this range were considered to have a NANP- or CT-skewed response (ratio > 1.25 shown in purple and ratio
    Figure Legend Snippet: High variability among RTS,S vaccine-induced IgG targeting the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, 3 M) were tested for IgG to NANP and CT and C1q-fixation to CSP, and the values were used for the following analysis. a Heat map of children arranged in descending (top to bottom) order of C1q-fixation (left), corresponding IgG to NANP and CT (middle), and for comparison IgM to NANP and CT (right). b Variability between epitope specificity was quantified by calculating the ratio of NANP-to-CT IgG. Children with low variability were considered to have equal reactivity to NANP and CT (ratio between 0.75 and 1.25 shown in white), and children exceeding this range were considered to have a NANP- or CT-skewed response (ratio > 1.25 shown in purple and ratio

    Techniques Used:

    Functional complement-fixing antibodies target the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça (black box plots; N = 50) and Ilha Josina cohorts (gray box plots; N = 49) were tested for IgG ( a ) and IgM ( b ) to NANP and CT regions of CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate, and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between paired samples was compared using Wilcoxon matched-pairs signed-rank test. c , d Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, M3) were tested for C1q-fixation to CSP, NANP, and CT, and the values were plotted compared to IgG reactivity (c) and C1q fixation to CSP, NANP, and CT were correlated (d)
    Figure Legend Snippet: Functional complement-fixing antibodies target the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça (black box plots; N = 50) and Ilha Josina cohorts (gray box plots; N = 49) were tested for IgG ( a ) and IgM ( b ) to NANP and CT regions of CSP. Sera collected at baseline (month 0, M0) and after vaccination (month 3, M3) were tested in duplicate, and the mean value was used to generate box plots whereby top, center, and bottom horizontal lines represent the 75th percentile, median, and 25th percentile, respectively; upper and lower whiskers represent the highest and lowest values within 1.5× IQR, respectively; and values that exceed this range are presented as dots. Malaria-naïve negative controls from Melbourne donors were used to calculate the seropositivity cutoff values (dashed lines), and the percentages of individuals above this threshold are shown. Reactivity between paired samples was compared using Wilcoxon matched-pairs signed-rank test. c , d Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts ( N = 99, M3) were tested for C1q-fixation to CSP, NANP, and CT, and the values were plotted compared to IgG reactivity (c) and C1q fixation to CSP, NANP, and CT were correlated (d)

    Techniques Used: Functional Assay

    39) Product Images from "Impact of age and vaccination history on long-term serological responses after symptomatic B. pertussis infection, a high dimensional data analysis"

    Article Title: Impact of age and vaccination history on long-term serological responses after symptomatic B. pertussis infection, a high dimensional data analysis

    Journal: Scientific Reports

    doi: 10.1038/srep40328

    Measured Ig levels and model parameters in IgG and IgA datasets of all SKI cases. An example is given of density plots showing distributions for the model parameters (A, B, k a and k b ) describing the measured IgG-Ptx dataset of all SKI cases: A and B components (at day 28, in IU/ml) (left panels), and their respective half-lives (days) (right panels) ( a ). Dot plots show measured IgG and IgA levels to Ptx, FHA, and Prn (in IU/ml) and to Fim2/3 and OMV (in AU/ml) of all SKI cases as a function of time after exposure (in days, logarithmic scale). Open circles represent measured IgG and IgA levels, lines indicate 5 th , 50 th (medians) and 95 th percentiles of the modelled responses. The IgG-OMV dot plot represents data of the IgG subclass IgG3 in AU/ml. ( b ).
    Figure Legend Snippet: Measured Ig levels and model parameters in IgG and IgA datasets of all SKI cases. An example is given of density plots showing distributions for the model parameters (A, B, k a and k b ) describing the measured IgG-Ptx dataset of all SKI cases: A and B components (at day 28, in IU/ml) (left panels), and their respective half-lives (days) (right panels) ( a ). Dot plots show measured IgG and IgA levels to Ptx, FHA, and Prn (in IU/ml) and to Fim2/3 and OMV (in AU/ml) of all SKI cases as a function of time after exposure (in days, logarithmic scale). Open circles represent measured IgG and IgA levels, lines indicate 5 th , 50 th (medians) and 95 th percentiles of the modelled responses. The IgG-OMV dot plot represents data of the IgG subclass IgG3 in AU/ml. ( b ).

    Techniques Used:

    Impact of age group factors on IgG subclass levels against B. pertussis specific antigens. Median levels of IgG subclasses IgG1 ( a ), IgG2 ( b ), IgG3 ( c ) and IgG4 ( d ) to B. pertussis specific antigens predicted by our biexponential decay model for six time points, i.e. day 28, day 70 (week 10), day 140 (week 20), day 365 (year 1), day 730 (year 2), day 1095 (year 3) (shown in days, non-linear time axis), for each age group, as indicated.
    Figure Legend Snippet: Impact of age group factors on IgG subclass levels against B. pertussis specific antigens. Median levels of IgG subclasses IgG1 ( a ), IgG2 ( b ), IgG3 ( c ) and IgG4 ( d ) to B. pertussis specific antigens predicted by our biexponential decay model for six time points, i.e. day 28, day 70 (week 10), day 140 (week 20), day 365 (year 1), day 730 (year 2), day 1095 (year 3) (shown in days, non-linear time axis), for each age group, as indicated.

    Techniques Used:

    Predicted Ig levels against B. pertussis specific antigens stratified by age group. Median IgG ( a ) and IgA ( b ) values to B. pertussis specific antigens predicted by our biexponential decay model for six time points, i.e. day 28, day 70 (week 10), day 140 (week 20), day 365 (year 1), day 730 (year 2), day 1095 (year 3) (shown in days, non-linear time axis), for each age group, as indicated.
    Figure Legend Snippet: Predicted Ig levels against B. pertussis specific antigens stratified by age group. Median IgG ( a ) and IgA ( b ) values to B. pertussis specific antigens predicted by our biexponential decay model for six time points, i.e. day 28, day 70 (week 10), day 140 (week 20), day 365 (year 1), day 730 (year 2), day 1095 (year 3) (shown in days, non-linear time axis), for each age group, as indicated.

    Techniques Used:

    Priming with aP or wP vaccination leads to opposing trends in B. pertussis specific IgG3 and IgG4 responses after pertussis infection. Scatter dot plots of specific IgG3 (left panels) and IgG4 (right panels) concentrations of Ptx ( a , b ), FHA ( c , d ), Prn ( e , f ), Fim2/3 ( g , h ) and OMV ( i , j ) in under-fours (aP primed; u4s), schoolchildren (aP primed and aP boosted; sch aP/aP), schoolchildren (wP primed and aP boosted; sch wP/aP) and adolescents (wP primed, not boosted; ado) early (≤9 months) and late (9–48 months) after last known B. pertussis antigen exposure. Non vaccinated subjects were excluded. *p
    Figure Legend Snippet: Priming with aP or wP vaccination leads to opposing trends in B. pertussis specific IgG3 and IgG4 responses after pertussis infection. Scatter dot plots of specific IgG3 (left panels) and IgG4 (right panels) concentrations of Ptx ( a , b ), FHA ( c , d ), Prn ( e , f ), Fim2/3 ( g , h ) and OMV ( i , j ) in under-fours (aP primed; u4s), schoolchildren (aP primed and aP boosted; sch aP/aP), schoolchildren (wP primed and aP boosted; sch wP/aP) and adolescents (wP primed, not boosted; ado) early (≤9 months) and late (9–48 months) after last known B. pertussis antigen exposure. Non vaccinated subjects were excluded. *p

    Techniques Used: Infection

    40) Product Images from "Intradermal grass pollen immunotherapy increases TH2 and IgE responses and worsens respiratory allergic symptoms"

    Article Title: Intradermal grass pollen immunotherapy increases TH2 and IgE responses and worsens respiratory allergic symptoms

    Journal: The Journal of Allergy and Clinical Immunology

    doi: 10.1016/j.jaci.2016.09.024

    Immunologic outcomes. A, Levels of P pratense –specific IgE and IgG before and after completion of 7 intradermal allergen or histamine control injections. B, Expression of CRTH2 (T H 2 marker) and CXCR3 (T H 1 marker) on CD4 + cells expanded from skin biopsy specimens (24 hours after skin challenge). C, Areas of cutaneous late-phase responses (24 hours after intradermal skin challenge) 4 months and either 7, 10, or 13 months after treatment (September 2013). Late-phase response suppression was shown in our previous study (Rotiroti et al 5 ) immediately after 6 biweekly intradermal injections. Solid bars represent median values. P values for pretreatment and posttreatment serology comparisons are based on the Wilcoxon signed-rank test, and those for between-group IgE and IgG levels are based on analysis of covariance. P values in Fig 4, B and C , are based on the Mann-Whitney U test.
    Figure Legend Snippet: Immunologic outcomes. A, Levels of P pratense –specific IgE and IgG before and after completion of 7 intradermal allergen or histamine control injections. B, Expression of CRTH2 (T H 2 marker) and CXCR3 (T H 1 marker) on CD4 + cells expanded from skin biopsy specimens (24 hours after skin challenge). C, Areas of cutaneous late-phase responses (24 hours after intradermal skin challenge) 4 months and either 7, 10, or 13 months after treatment (September 2013). Late-phase response suppression was shown in our previous study (Rotiroti et al 5 ) immediately after 6 biweekly intradermal injections. Solid bars represent median values. P values for pretreatment and posttreatment serology comparisons are based on the Wilcoxon signed-rank test, and those for between-group IgE and IgG levels are based on analysis of covariance. P values in Fig 4, B and C , are based on the Mann-Whitney U test.

    Techniques Used: Expressing, Marker, MANN-WHITNEY

    Related Articles

    Acetylene Reduction Assay:

    Article Title: Analysis of Cytokine Production by Peanut-Reactive T Cells Identifies Residual Th2 Effectors in Highly Allergic Children Who Received Peanut Oral Immunotherapy
    Article Snippet: .. Specific IgE and IgG4 antibodies (ab) to whole peanut (f13) as well as Ara h 1 (f422) and Ara h 2 (f423) were measured by ImmunoCAP assay (Phadia US, Portage, MI) using the ImmunoCAP 250 system. ..

    Purification:

    Article Title: Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes
    Article Snippet: .. Quantification of total protein in plasma and purified IgA, IgG and IgG4 fractions To determine the protein concentration of plasma and purified IgA, IgG and IgG4 fractions, a Bradford protein assay kit (Thermo Scientific, Rockford, USA) was used according to the manufacturer’s instructions. ..

    Incubation:

    Article Title: Glycoproteins in circulating immune complexes are biomarkers of patients with Indian PKDL: A study from endemic districts of West Bengal, India
    Article Snippet: .. The wells were then incubated for 1hr at RT with HRP-conjugated anti-human monoclonal IgG (1:15,000) (Sigma-Aldrich, Cat # :A0170), monoclonal IgG1 (1:1000) (Thermo Fisher Scientific, Cat#: A-10648, USA), monoclonal IgG2 (1:1000) (Thermo Fisher Scientific, Cat#:MH1772, USA), monoclonal IgG3 (1:1000) (Thermo Fisher Scientific, Cat#:05–3620,USA), monoclonal IgG4 (1:1000) (Thermo Fisher Scientific, Cat#:A-10654,USA), and polyclonal IgM (1:10,000) (Sigma-Aldrich, Cat#:A6907), respectively, to measure the levels of IgM, IgG and IgG subclasses of anti-leishmanial antibodies with Tetramethylbenzidine (TMB). .. Optical density (OD) was measured at 450 nm on a micro plate reader (Erba Lisa Scan II, Germany).

    Article Title: Development of an IgG4-based Classifier/Predictor of Endemic Pemphigus Foliaceus (Fogo Selvagem)
    Article Snippet: .. Following wash, the plate was incubated with a 1:2000 dilution (for IgG plates) 1:1000 dilution (for IgG subclasses) of a mouse horseradish peroxidase (HRP) labelled monoclonal antibody against human IgG or human IgG1, IgG2, IgG3 and IgG4 subclasses (Zymed, San Francisco, California) ( ; ). ..

    other:

    Article Title: Phase 1 results of safety and tolerability in a rush oral immunotherapy protocol to multiple foods using Omalizumab
    Article Snippet: Sera were analyzed for peanut-specific IgE and IgG4 levels at John Hopkins Allergy and Clinical Immunology Reference Laboratory by immunoCAP FEIA (Thermofisher Scientific/Phadia, Kalmazoo, MI).

    Immunocap ISAC Assay:

    Article Title: Analysis of Cytokine Production by Peanut-Reactive T Cells Identifies Residual Th2 Effectors in Highly Allergic Children Who Received Peanut Oral Immunotherapy
    Article Snippet: .. Specific IgE and IgG4 antibodies (ab) to whole peanut (f13) as well as Ara h 1 (f422) and Ara h 2 (f423) were measured by ImmunoCAP assay (Phadia US, Portage, MI) using the ImmunoCAP 250 system. ..

    Bradford Protein Assay:

    Article Title: Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes
    Article Snippet: .. Quantification of total protein in plasma and purified IgA, IgG and IgG4 fractions To determine the protein concentration of plasma and purified IgA, IgG and IgG4 fractions, a Bradford protein assay kit (Thermo Scientific, Rockford, USA) was used according to the manufacturer’s instructions. ..

    Protein Concentration:

    Article Title: Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes
    Article Snippet: .. Quantification of total protein in plasma and purified IgA, IgG and IgG4 fractions To determine the protein concentration of plasma and purified IgA, IgG and IgG4 fractions, a Bradford protein assay kit (Thermo Scientific, Rockford, USA) was used according to the manufacturer’s instructions. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Glycoproteins in circulating immune complexes are biomarkers of patients with Indian PKDL: A study from endemic districts of West Bengal, India
    Article Snippet: .. The wells were then incubated for 1hr at RT with HRP-conjugated anti-human monoclonal IgG (1:15,000) (Sigma-Aldrich, Cat # :A0170), monoclonal IgG1 (1:1000) (Thermo Fisher Scientific, Cat#: A-10648, USA), monoclonal IgG2 (1:1000) (Thermo Fisher Scientific, Cat:MH1772, USA), monoclonal IgG3 (1:1000) (Thermo Fisher Scientific, Cat#:05–3620,USA), monoclonal IgG4 (1:1000) (Thermo Fisher Scientific, Cat#:A-10654,USA), and polyclonal IgM (1:10,000) (Sigma-Aldrich, Cat#:A6907), respectively, to measure the levels of IgM, IgG and IgG subclasses of anti-leishmanial antibodies with Tetramethylbenzidine (TMB). .. Optical density (OD) was measured at 450 nm on a micro plate reader (Erba Lisa Scan II, Germany).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Thermo Fisher flow cytometry polyclonal igg anti np
    <t>IgG</t> anti-NP suppresses IgM anti-NP responses at all NP densities. C57BL/6 mice were immunized i.v. with 5 × 10 7 SRBC-NP with high (SRBC-NP high ), intermediate (SRBC-NP int ) or low (SRBC-NP low ) epitope density with or without 30 μg <t>polyclonal</t> IgG anti-NP. Mice immunized with 5 × 10 7 unconjugated SRBC or with 30 μg IgG-anti NP only were used as controls. NP-specific immune responses were analyzed in spleen and serum samples obtained 5 days after immunization. ( A ) Cells were initially gated for B220 + cells. Representative flow <t>cytometry</t> gating for B220 + GC (defined as GL7 high CD95 high ) and non-GC λ1 + NP + cells from mice immunized with SRBC-NP high (left), IgG anti-NP + SRBC-NP high (middle) and IgG anti-NP alone (right). ( B ) Frequency of GC and non-GC NP + λ1 + cells in total B220 + cells. ( C ) NP-specific IgM-secreting cells per spleen. ( D ) Serum IgM anti-NP levels (serum dilution in ELISA = 1:625). The dashed line indicates the mean value of mice immunized with unconjugated SRBC. Representative of four independent experiments with 4–5 mice per group. ns = p > 0.05, * p
    Flow Cytometry Polyclonal Igg Anti Np, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry polyclonal igg anti np/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flow cytometry polyclonal igg anti np - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Thermo Fisher magnabind immunoglobulin g igg beads
    (a) Subcellular localization of MR and RACK1 in cultured M1-rMR TAT3-Gluc cells. Serum-starved cells were treated with vehicle or aldosterone for 3 hours. Fixed cells were incubated with mouse anti-MR and rabbit anti-RACK1, followed by goat anti-mouse Alexa Fluor 594 (red) and goat anti-rabbit Alexa Fluor 488 (green). RACK1 colocalized with MR in the presence of aldosterone in the nuclei of the transfected M1-CCD cells. (b) Colocalization of RACK1 (green) and MR (red) in rat brain. Paraffin-embedded tissue was incubated with mouse anti-MR antibody, followed by Alexa Fluor 594–conjugated anti-mouse <t>IgG</t> (red) and rabbit anti-RACK1 antibody, followed by Alexa Fluor 488–conjugated anti-rabbit IgG (green). Nuclear staining was performed by using 4′,6-diamidino-2-phenylindole. Scale bar = 50 µM.
    Magnabind Immunoglobulin G Igg Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnabind immunoglobulin g igg beads/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    magnabind immunoglobulin g igg beads - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Thermo Fisher goat anti mouse igg1
    Chop negatively regulates T-bet expression. a Time-dependent expression (upper panel) and corresponding densitometry quantitation (lower panel) of T-bet in primed wild-type and Ddit3 −/− CD8 + T cells. Left: protein level (0–72 h); right: Tbx21 mRNA levels 48 h post-activation. CD8 + T cells were stimulated with plate-bound anti-CD3/CD28 ( n = 3). b Tbx21 and Ifng mRNA expression in activated CD8 + T cells infected with control retrovirus (Retro-Ctrl) or Ddit3 -expressing retrovirus (Retro-Chop). Cells were primed for 48 h and then infected for additional 48 h in the presence of the stimulating anti-CD3/CD28 antibodies ( n = 4). c Ifng , Il12b2 , Cbfa3 , and Cxcr3 mRNA levels in control vs. Ddit3 −/− CD8 + T cells primed as in a ( n = 5). d Predicted Chop-binding site in the Tbx21 promoter region (GGGTGCAATCTTC). e Chromatin immunoprecipitation assay for the endogenous binding of Chop to Tbx21 promoter in primed wild-type or Ddit3 −/− CD8 + T cells. Chop-binding activity was measured by real-time quantitative PCR, compared with <t>IgG</t> binding activity after normalizing to the activity of anti-H3 ( n = 4). f A dual luciferase system composed of 2x-CRE containing Firefly luciferase reporter and the control Renilla luciferase reporter was transfected into 293T cells in combination with Ddit3 -expressing or control vectors. n = 4 experimental repeats. g Expression of Chop (left) and T-bet (right) by fluorescence-activated cell sorter (FACS) upon transduction of primed CD8 + T cells with green fluorescent protein (GFP)-coding retroviruses containing control or 8x-CRE sequences. Cells were primed for 48 h and then infected for another 48 h in the presence of the stimulating anti-CD3/CD28 antibodies plus interleukin (IL)-2 (50 U/ml). n = 3 independent repeats. h Interferon-γ (IFNγ) levels in primed CD8 + T cells transduced with: (1) GFP/CD90.1-expressing control virus (Ctrl); (2) Chop/CD90.1-expressing virus and GFP-expressing control virus (Chop); (3) CD90.1-expressing control virus and T-bet/GFP-expressing virus (T-bet); or (4) Chop/CD90.1-expressing virus and T-bet/GFP-expressing virus (Chop/T-bet). Cells were primed for 24 h and then transduced for additional 72 h in the presence of stimulating antibodies plus IL-2 (50 U/ml). Then IFNγ levels were detected by FACS in gated CD90.1 + GFP + cells. Right: Representative FACS result; left: Merged results from three independent experiments. In the bar graphs showing mean ± s.e.m., * p
    Goat Anti Mouse Igg1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg1/product/Thermo Fisher
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg1 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Thermo Fisher igg anti ttg
    Histogram comparing standard diagnosis by <t>IgG</t> <t>anti-tTG</t> ELISA assay and/or biopsy and/or follow-up to CD-LFIA test results.
    Igg Anti Ttg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg anti ttg/product/Thermo Fisher
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    igg anti ttg - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    IgG anti-NP suppresses IgM anti-NP responses at all NP densities. C57BL/6 mice were immunized i.v. with 5 × 10 7 SRBC-NP with high (SRBC-NP high ), intermediate (SRBC-NP int ) or low (SRBC-NP low ) epitope density with or without 30 μg polyclonal IgG anti-NP. Mice immunized with 5 × 10 7 unconjugated SRBC or with 30 μg IgG-anti NP only were used as controls. NP-specific immune responses were analyzed in spleen and serum samples obtained 5 days after immunization. ( A ) Cells were initially gated for B220 + cells. Representative flow cytometry gating for B220 + GC (defined as GL7 high CD95 high ) and non-GC λ1 + NP + cells from mice immunized with SRBC-NP high (left), IgG anti-NP + SRBC-NP high (middle) and IgG anti-NP alone (right). ( B ) Frequency of GC and non-GC NP + λ1 + cells in total B220 + cells. ( C ) NP-specific IgM-secreting cells per spleen. ( D ) Serum IgM anti-NP levels (serum dilution in ELISA = 1:625). The dashed line indicates the mean value of mice immunized with unconjugated SRBC. Representative of four independent experiments with 4–5 mice per group. ns = p > 0.05, * p

    Journal: Scientific Reports

    Article Title: IgG-mediated immune suppression in mice is epitope specific except during high epitope density conditions

    doi: 10.1038/s41598-018-33087-6

    Figure Lengend Snippet: IgG anti-NP suppresses IgM anti-NP responses at all NP densities. C57BL/6 mice were immunized i.v. with 5 × 10 7 SRBC-NP with high (SRBC-NP high ), intermediate (SRBC-NP int ) or low (SRBC-NP low ) epitope density with or without 30 μg polyclonal IgG anti-NP. Mice immunized with 5 × 10 7 unconjugated SRBC or with 30 μg IgG-anti NP only were used as controls. NP-specific immune responses were analyzed in spleen and serum samples obtained 5 days after immunization. ( A ) Cells were initially gated for B220 + cells. Representative flow cytometry gating for B220 + GC (defined as GL7 high CD95 high ) and non-GC λ1 + NP + cells from mice immunized with SRBC-NP high (left), IgG anti-NP + SRBC-NP high (middle) and IgG anti-NP alone (right). ( B ) Frequency of GC and non-GC NP + λ1 + cells in total B220 + cells. ( C ) NP-specific IgM-secreting cells per spleen. ( D ) Serum IgM anti-NP levels (serum dilution in ELISA = 1:625). The dashed line indicates the mean value of mice immunized with unconjugated SRBC. Representative of four independent experiments with 4–5 mice per group. ns = p > 0.05, * p

    Article Snippet: Flow cytometry Polyclonal IgG anti-NP (prepared as described above) was biotinylated using EZ-Link Sulfo-NHS-LC- LC-Biotin (sulfosuccinimidyl-6-biotinamido-6-hexanamido hexaonate) (Thermo Scientific) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    (a) Subcellular localization of MR and RACK1 in cultured M1-rMR TAT3-Gluc cells. Serum-starved cells were treated with vehicle or aldosterone for 3 hours. Fixed cells were incubated with mouse anti-MR and rabbit anti-RACK1, followed by goat anti-mouse Alexa Fluor 594 (red) and goat anti-rabbit Alexa Fluor 488 (green). RACK1 colocalized with MR in the presence of aldosterone in the nuclei of the transfected M1-CCD cells. (b) Colocalization of RACK1 (green) and MR (red) in rat brain. Paraffin-embedded tissue was incubated with mouse anti-MR antibody, followed by Alexa Fluor 594–conjugated anti-mouse IgG (red) and rabbit anti-RACK1 antibody, followed by Alexa Fluor 488–conjugated anti-rabbit IgG (green). Nuclear staining was performed by using 4′,6-diamidino-2-phenylindole. Scale bar = 50 µM.

    Journal: Endocrinology

    Article Title: Interaction of the Mineralocorticoid Receptor With RACK1 and Its Role in Aldosterone Signaling

    doi: 10.1210/en.2017-00095

    Figure Lengend Snippet: (a) Subcellular localization of MR and RACK1 in cultured M1-rMR TAT3-Gluc cells. Serum-starved cells were treated with vehicle or aldosterone for 3 hours. Fixed cells were incubated with mouse anti-MR and rabbit anti-RACK1, followed by goat anti-mouse Alexa Fluor 594 (red) and goat anti-rabbit Alexa Fluor 488 (green). RACK1 colocalized with MR in the presence of aldosterone in the nuclei of the transfected M1-CCD cells. (b) Colocalization of RACK1 (green) and MR (red) in rat brain. Paraffin-embedded tissue was incubated with mouse anti-MR antibody, followed by Alexa Fluor 594–conjugated anti-mouse IgG (red) and rabbit anti-RACK1 antibody, followed by Alexa Fluor 488–conjugated anti-rabbit IgG (green). Nuclear staining was performed by using 4′,6-diamidino-2-phenylindole. Scale bar = 50 µM.

    Article Snippet: For immunoprecipitation, 500 µg of whole-cell lysate was incubated overnight with a rat MR rabbit polyclonal antibody (N-terminal) on a rocker, and then MagnaBind immunoglobulin G (IgG) beads were added to the antibody lysate mixture for 3 hours (Thermo Fisher Scientific).

    Techniques: Cell Culture, Incubation, Transfection, Staining

    Chop negatively regulates T-bet expression. a Time-dependent expression (upper panel) and corresponding densitometry quantitation (lower panel) of T-bet in primed wild-type and Ddit3 −/− CD8 + T cells. Left: protein level (0–72 h); right: Tbx21 mRNA levels 48 h post-activation. CD8 + T cells were stimulated with plate-bound anti-CD3/CD28 ( n = 3). b Tbx21 and Ifng mRNA expression in activated CD8 + T cells infected with control retrovirus (Retro-Ctrl) or Ddit3 -expressing retrovirus (Retro-Chop). Cells were primed for 48 h and then infected for additional 48 h in the presence of the stimulating anti-CD3/CD28 antibodies ( n = 4). c Ifng , Il12b2 , Cbfa3 , and Cxcr3 mRNA levels in control vs. Ddit3 −/− CD8 + T cells primed as in a ( n = 5). d Predicted Chop-binding site in the Tbx21 promoter region (GGGTGCAATCTTC). e Chromatin immunoprecipitation assay for the endogenous binding of Chop to Tbx21 promoter in primed wild-type or Ddit3 −/− CD8 + T cells. Chop-binding activity was measured by real-time quantitative PCR, compared with IgG binding activity after normalizing to the activity of anti-H3 ( n = 4). f A dual luciferase system composed of 2x-CRE containing Firefly luciferase reporter and the control Renilla luciferase reporter was transfected into 293T cells in combination with Ddit3 -expressing or control vectors. n = 4 experimental repeats. g Expression of Chop (left) and T-bet (right) by fluorescence-activated cell sorter (FACS) upon transduction of primed CD8 + T cells with green fluorescent protein (GFP)-coding retroviruses containing control or 8x-CRE sequences. Cells were primed for 48 h and then infected for another 48 h in the presence of the stimulating anti-CD3/CD28 antibodies plus interleukin (IL)-2 (50 U/ml). n = 3 independent repeats. h Interferon-γ (IFNγ) levels in primed CD8 + T cells transduced with: (1) GFP/CD90.1-expressing control virus (Ctrl); (2) Chop/CD90.1-expressing virus and GFP-expressing control virus (Chop); (3) CD90.1-expressing control virus and T-bet/GFP-expressing virus (T-bet); or (4) Chop/CD90.1-expressing virus and T-bet/GFP-expressing virus (Chop/T-bet). Cells were primed for 24 h and then transduced for additional 72 h in the presence of stimulating antibodies plus IL-2 (50 U/ml). Then IFNγ levels were detected by FACS in gated CD90.1 + GFP + cells. Right: Representative FACS result; left: Merged results from three independent experiments. In the bar graphs showing mean ± s.e.m., * p

    Journal: Nature Communications

    Article Title: ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression

    doi: 10.1038/s41467-019-09263-1

    Figure Lengend Snippet: Chop negatively regulates T-bet expression. a Time-dependent expression (upper panel) and corresponding densitometry quantitation (lower panel) of T-bet in primed wild-type and Ddit3 −/− CD8 + T cells. Left: protein level (0–72 h); right: Tbx21 mRNA levels 48 h post-activation. CD8 + T cells were stimulated with plate-bound anti-CD3/CD28 ( n = 3). b Tbx21 and Ifng mRNA expression in activated CD8 + T cells infected with control retrovirus (Retro-Ctrl) or Ddit3 -expressing retrovirus (Retro-Chop). Cells were primed for 48 h and then infected for additional 48 h in the presence of the stimulating anti-CD3/CD28 antibodies ( n = 4). c Ifng , Il12b2 , Cbfa3 , and Cxcr3 mRNA levels in control vs. Ddit3 −/− CD8 + T cells primed as in a ( n = 5). d Predicted Chop-binding site in the Tbx21 promoter region (GGGTGCAATCTTC). e Chromatin immunoprecipitation assay for the endogenous binding of Chop to Tbx21 promoter in primed wild-type or Ddit3 −/− CD8 + T cells. Chop-binding activity was measured by real-time quantitative PCR, compared with IgG binding activity after normalizing to the activity of anti-H3 ( n = 4). f A dual luciferase system composed of 2x-CRE containing Firefly luciferase reporter and the control Renilla luciferase reporter was transfected into 293T cells in combination with Ddit3 -expressing or control vectors. n = 4 experimental repeats. g Expression of Chop (left) and T-bet (right) by fluorescence-activated cell sorter (FACS) upon transduction of primed CD8 + T cells with green fluorescent protein (GFP)-coding retroviruses containing control or 8x-CRE sequences. Cells were primed for 48 h and then infected for another 48 h in the presence of the stimulating anti-CD3/CD28 antibodies plus interleukin (IL)-2 (50 U/ml). n = 3 independent repeats. h Interferon-γ (IFNγ) levels in primed CD8 + T cells transduced with: (1) GFP/CD90.1-expressing control virus (Ctrl); (2) Chop/CD90.1-expressing virus and GFP-expressing control virus (Chop); (3) CD90.1-expressing control virus and T-bet/GFP-expressing virus (T-bet); or (4) Chop/CD90.1-expressing virus and T-bet/GFP-expressing virus (Chop/T-bet). Cells were primed for 24 h and then transduced for additional 72 h in the presence of stimulating antibodies plus IL-2 (50 U/ml). Then IFNγ levels were detected by FACS in gated CD90.1 + GFP + cells. Right: Representative FACS result; left: Merged results from three independent experiments. In the bar graphs showing mean ± s.e.m., * p

    Article Snippet: After de-paraffinization and antigen retrieval, sections were blocked in 5% goat serum and incubated overnight with mouse monoclonal anti-CD8 (1:100, IgG1, C8/144B, #108M-98, Cell Marque) and mouse monoclonal anti-Chop (1:100, IgG2b, 9C8, #ab11419, Abcam) or mouse monoclonal anti-CD8 and rabbit polyclonal anti-CHOP/GADD153 (1:500, R-20, #sc-793, Santa Cruz Biotechnologies), followed by washes in PBS and incubation in secondary goat anti-mouse IgG1 and IgG2b or goat anti-mouse IgG1 and anti-rabbit IgG labeled with Alexa Fluor® 488 and 647 (all 1:200, ThermoFisher Scientific), respectively.

    Techniques: Expressing, Quantitation Assay, Activation Assay, Infection, Binding Assay, Chromatin Immunoprecipitation, Activity Assay, Real-time Polymerase Chain Reaction, Luciferase, Transfection, Fluorescence, FACS, Transduction

    Histogram comparing standard diagnosis by IgG anti-tTG ELISA assay and/or biopsy and/or follow-up to CD-LFIA test results.

    Journal: BMC Gastroenterology

    Article Title: Early diagnosis of celiac disease in IgA deficient children: contribution of a point-of-care test

    doi: 10.1186/1471-230X-14-186

    Figure Lengend Snippet: Histogram comparing standard diagnosis by IgG anti-tTG ELISA assay and/or biopsy and/or follow-up to CD-LFIA test results.

    Article Snippet: In case of discrepancy between IgG anti-tTG and CD-LFIA, IgG anti-DGP levels were measured by the ELISA test Varelisa® Gliadin Antibodies from Thermo Fisher (Uppsala, Sweden).

    Techniques: Enzyme-linked Immunosorbent Assay