igg4 igg subclasses  (SouthernBiotech)

 
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  • 94
    Name:
    Mouse Anti Human IgG4 pFc UNLB
    Description:

    Catalog Number:
    9190-01
    Price:
    None
    Applications:
    Applications for relevant formats of this clone include -ELISA – Quality tested 2-9FLISA – Quality testedFlow Cytometry – Reported in literature 12-14Immunohistochemistry-Frozen Sections – Reported in literature 10Western Blot – Reported in literature 11Immunoprecipitation – Reported in literature 1Surface Plasmon Resonance – Reported in literature 15
    Isotype:
    Mouse (BALB/c) IgG3κ
    Format:
    UNLB (Unconjugated)
    Buy from Supplier


    Structured Review

    SouthernBiotech igg4 igg subclasses
    Mouse Anti Human IgG4 pFc UNLB

    https://www.bioz.com/result/igg4 igg subclasses/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg4 igg subclasses - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "Delayed fractional dose regimen of the RTS,S/AS01 malaria vaccine candidate enhances an IgG4 response that inhibits serum opsonophagocytosis"

    Article Title: Delayed fractional dose regimen of the RTS,S/AS01 malaria vaccine candidate enhances an IgG4 response that inhibits serum opsonophagocytosis

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-08526-5

    IgG subclass titers for 012M and Fx017M cohorts. Antibody titers for IgG1, IgG2, IgG3, and IgG4 for the 012M and Fx017M cohorts against three CSP test antigens: full-length recombinant CSP (left column), NANP repeat region peptide (middle column), and C-terminal PF16 peptide (right column). Protected and non-protected subjects are colored in blue and red, respectively.
    Figure Legend Snippet: IgG subclass titers for 012M and Fx017M cohorts. Antibody titers for IgG1, IgG2, IgG3, and IgG4 for the 012M and Fx017M cohorts against three CSP test antigens: full-length recombinant CSP (left column), NANP repeat region peptide (middle column), and C-terminal PF16 peptide (right column). Protected and non-protected subjects are colored in blue and red, respectively.

    Techniques Used: Recombinant

    Serum opsonophagocytic activity following depletion of IgG isotype subclass antibodies. The change in peak OPA relative to the non-depleted sample, as measured by Mfreq, when the low-OPA and high-OPA pools from the 012M and Fx017M cohorts were depleted of IgG1, IgG3, IgG4, or IgG1 + IgG3.
    Figure Legend Snippet: Serum opsonophagocytic activity following depletion of IgG isotype subclass antibodies. The change in peak OPA relative to the non-depleted sample, as measured by Mfreq, when the low-OPA and high-OPA pools from the 012M and Fx017M cohorts were depleted of IgG1, IgG3, IgG4, or IgG1 + IgG3.

    Techniques Used: Activity Assay

    Serum opsonophagocytic activity following depletion of IgG isotype subclasses at a range of serum concentrations. The change in Mfreq relative to the non-depleted sample is shown following depletion of IgG1, IgG3, and IgG4 for the low-OPA and high-OPA pools for the 012M and Fx017M cohorts.
    Figure Legend Snippet: Serum opsonophagocytic activity following depletion of IgG isotype subclasses at a range of serum concentrations. The change in Mfreq relative to the non-depleted sample is shown following depletion of IgG1, IgG3, and IgG4 for the low-OPA and high-OPA pools for the 012M and Fx017M cohorts.

    Techniques Used: Activity Assay

    Related Articles

    Immunohistochemistry:

    Article Title: The distribution of IgG subclass deposition on renal tissues from patients with anti-glomerular basement membrane disease
    Article Snippet: .. Immunohistochemistry for anti-GBM IgG subclasses on renal biopsy sections Paraffin-embedded specimens of formalin-fixed renal biopsy tissue were processed for immunohistochemical staining for IgG subclasses using mouse monoclonal antibodies against human IgG1, IgG2, IgG3 and IgG4 (γ chains specific, Southern Biotech, Birmingham, USA). ..

    Cell Culture:

    Article Title: Role of CYP2E1 Immunoglobulin G4 Subclass Antibodies and Complement in Pathogenesis of Idiosyncratic Drug-Induced Hepatitis
    Article Snippet: .. Alkaline phosphatase-conjugated mouse anti-human IgG1, clone 4E3, isotype mouse IgG1, mouse anti-human IgG2, clone 31-74, isotype mouse IgG1(κ), mouse anti-human IgG3, clone HP6050, isotype mouse IgG1(κ) and mouse anti-human IgG4, clone HP6025, isotype mouse IgG1(κ) were from Southern Biotech (Birmingham, AL); alkaline phosphatase substrate kit, from Bio-Rad (Hercules, CA); antiactin enzyme-linked immunosorbent assay (ELISA), anti-liver/kidney microsome 1 (LKM-1) ELISA, and soluble liver antigen (SLA) ELISA, from QUANTA Lite INOVA Diagnostics, Inc. (San Diego, CA); anti-dsDNA enzyme immunoassay (EIA), from Scimedx Corporation (Denville, NJ); ANA/HEp-2 cell culture immunofluorescence test system, from Zeus Scientific, Inc. (Raritan, NJ); C1q and C3d circulating immune complexes EIA kits, from ALPCO Diagnostics (Windham, NH); fetal calf serum, from Gibco-BRL, Life Technologies (Grand Island, NY); human C3a, C4a, and C5a ELISA kits from BD OptEIA, BD Biosciences (San Diego, CA); human CYP2E1 plus P450 reductase plus cytochrome b 5 Supersomes, from GenTest, BD Biosciences (Woburn, MA); human IgG subclass profile ELISA kit, from Zymed Laboratories, Inc. (San Francisco, CA); Immulon 2HB 96-well microtiter plates from ISC BioExpress (Kaysville, UT); phosphate-buffered saline (PBS), from Biofluids (Rockville, MD); and polyoxyethylene-sorbitan monolaurate (Tween 20), from Sigma (St. Louis, MO). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Role of CYP2E1 Immunoglobulin G4 Subclass Antibodies and Complement in Pathogenesis of Idiosyncratic Drug-Induced Hepatitis
    Article Snippet: .. Alkaline phosphatase-conjugated mouse anti-human IgG1, clone 4E3, isotype mouse IgG1, mouse anti-human IgG2, clone 31-74, isotype mouse IgG1(κ), mouse anti-human IgG3, clone HP6050, isotype mouse IgG1(κ) and mouse anti-human IgG4, clone HP6025, isotype mouse IgG1(κ) were from Southern Biotech (Birmingham, AL); alkaline phosphatase substrate kit, from Bio-Rad (Hercules, CA); antiactin enzyme-linked immunosorbent assay (ELISA), anti-liver/kidney microsome 1 (LKM-1) ELISA, and soluble liver antigen (SLA) ELISA, from QUANTA Lite INOVA Diagnostics, Inc. (San Diego, CA); anti-dsDNA enzyme immunoassay (EIA), from Scimedx Corporation (Denville, NJ); ANA/HEp-2 cell culture immunofluorescence test system, from Zeus Scientific, Inc. (Raritan, NJ); C1q and C3d circulating immune complexes EIA kits, from ALPCO Diagnostics (Windham, NH); fetal calf serum, from Gibco-BRL, Life Technologies (Grand Island, NY); human C3a, C4a, and C5a ELISA kits from BD OptEIA, BD Biosciences (San Diego, CA); human CYP2E1 plus P450 reductase plus cytochrome b 5 Supersomes, from GenTest, BD Biosciences (Woburn, MA); human IgG subclass profile ELISA kit, from Zymed Laboratories, Inc. (San Francisco, CA); Immulon 2HB 96-well microtiter plates from ISC BioExpress (Kaysville, UT); phosphate-buffered saline (PBS), from Biofluids (Rockville, MD); and polyoxyethylene-sorbitan monolaurate (Tween 20), from Sigma (St. Louis, MO). ..

    Concentration Assay:

    Article Title: Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques
    Article Snippet: .. Briefly, Microplates (NUNC) were coated overnight at 4°C with mouse monoclonal anti-human IgG (Southern Biotech) at a concentration of 1 μg/ml diluted in PBS. ..

    Article Title: Infectious Virion Capture by HIV-1 gp120-Specific IgG from RV144 Vaccinees
    Article Snippet: .. Briefly, microplates (Nunc) were coated overnight at 4°C with mouse monoclonal anti-human IgG (Southern Biotech) at a concentration of 1 μg/ml diluted in phosphate-buffered saline (PBS). ..

    Article Title: Dynamic Antibody Specificities and Virion Concentrations in Circulating Immune Complexes in Acute to Chronic HIV-1 Infection ▿Dynamic Antibody Specificities and Virion Concentrations in Circulating Immune Complexes in Acute to Chronic HIV-1 Infection ▿ †
    Article Snippet: .. Briefly, microplates (Nunc) were coated overnight at 4°C with mouse monoclonal anti-human IgG (Southern Biotech, Birmingham, AL) at a concentration of 1 μg/ml diluted in PBS. ..

    Incubation:

    Article Title: Phospholipase A2 Receptor–Related Membranous Nephropathy and Mannan-Binding Lectin Deficiency
    Article Snippet: .. For detection of IgG subclasses and complement components, cryosections of biopsy specimens were incubated with the following antibodies: mouse monoclonal anti–human IgG1, IgG2, IgG3, and IgG4 (clone HP; Southern Biotech); rabbit polyclonal anti–human C1q (Dako); anti–human C3 complement (Dako); and monoclonal anti–human C5b-9 (Dako). .. Staining for PLA2R, C4d, factor B, and properdin was performed on paraffin sections using rabbit polyclonal to human PLA2R (Atlas Antibodies), rabbit monoclonal to human C4d (DB BIOTECH) and human factor B (Quidel), and rabbit polyclonal to human properdin (Biorbyt) as primary antibodies.

    Sequencing:

    Article Title: Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein
    Article Snippet: .. mAb isotype and gene sequence analysis The isotype and subclass of secreted antibodies were determined using murine anti-human IgG1, IgG2, IgG3 or IgG4 mouse antibodies conjugated with alkaline phosphatase (Southern Biotech). .. Antibody heavy- and light-chain variable region genes were sequenced from hybridoma lines that had been cloned biologically from flow cytometry.

    Staining:

    Article Title: The distribution of IgG subclass deposition on renal tissues from patients with anti-glomerular basement membrane disease
    Article Snippet: .. Immunohistochemistry for anti-GBM IgG subclasses on renal biopsy sections Paraffin-embedded specimens of formalin-fixed renal biopsy tissue were processed for immunohistochemical staining for IgG subclasses using mouse monoclonal antibodies against human IgG1, IgG2, IgG3 and IgG4 (γ chains specific, Southern Biotech, Birmingham, USA). ..

    Immunofluorescence:

    Article Title: Role of CYP2E1 Immunoglobulin G4 Subclass Antibodies and Complement in Pathogenesis of Idiosyncratic Drug-Induced Hepatitis
    Article Snippet: .. Alkaline phosphatase-conjugated mouse anti-human IgG1, clone 4E3, isotype mouse IgG1, mouse anti-human IgG2, clone 31-74, isotype mouse IgG1(κ), mouse anti-human IgG3, clone HP6050, isotype mouse IgG1(κ) and mouse anti-human IgG4, clone HP6025, isotype mouse IgG1(κ) were from Southern Biotech (Birmingham, AL); alkaline phosphatase substrate kit, from Bio-Rad (Hercules, CA); antiactin enzyme-linked immunosorbent assay (ELISA), anti-liver/kidney microsome 1 (LKM-1) ELISA, and soluble liver antigen (SLA) ELISA, from QUANTA Lite INOVA Diagnostics, Inc. (San Diego, CA); anti-dsDNA enzyme immunoassay (EIA), from Scimedx Corporation (Denville, NJ); ANA/HEp-2 cell culture immunofluorescence test system, from Zeus Scientific, Inc. (Raritan, NJ); C1q and C3d circulating immune complexes EIA kits, from ALPCO Diagnostics (Windham, NH); fetal calf serum, from Gibco-BRL, Life Technologies (Grand Island, NY); human C3a, C4a, and C5a ELISA kits from BD OptEIA, BD Biosciences (San Diego, CA); human CYP2E1 plus P450 reductase plus cytochrome b 5 Supersomes, from GenTest, BD Biosciences (Woburn, MA); human IgG subclass profile ELISA kit, from Zymed Laboratories, Inc. (San Francisco, CA); Immulon 2HB 96-well microtiter plates from ISC BioExpress (Kaysville, UT); phosphate-buffered saline (PBS), from Biofluids (Rockville, MD); and polyoxyethylene-sorbitan monolaurate (Tween 20), from Sigma (St. Louis, MO). ..

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  • 91
    SouthernBiotech igg subclass elisa
    <t>ELISA</t> titers in mice immunized with Pfs230C1. ELISA data for individual mice (circles) and medians (bar) are shown. Total anti-Pfs230C1 antibody levels are presented on a log scale of ELISA units (A), and <t>IgG</t> subclass results are given as the optical density at 405 nm (OD 405 ) (B). Dotted lines indicate the background signals.
    Igg Subclass Elisa, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg subclass elisa/product/SouthernBiotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg subclass elisa - by Bioz Stars, 2020-07
    91/100 stars
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    88
    SouthernBiotech igg subclass antibodies
    Aire −/− mice have an increased specific response against TI-II antigen. ( A ) Serum levels of TNP-specific <t>IgM</t> and <t>IgG3</t> on day 7 after immunization of Aire −/− mice and wt littermate controls with TNP-Ficoll, determined by
    Igg Subclass Antibodies, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg subclass antibodies/product/SouthernBiotech
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    igg subclass antibodies - by Bioz Stars, 2020-07
    88/100 stars
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    93
    SouthernBiotech igg subclasses
    Vaccine elicited anti-DIII antibodies . Total <t>IgG</t> titers were measured by ELISA on WNV DIII-coated plates from mice vaccinated ID or IM with DNA plasmids encoding sections of the WNV E gene, with or without molecular adjutant P28 on week 8. Each dot represents an individual mouse. Undetectable antibody titers were arbitrarily assigned a titer of 1. Error bars denote the standard error within the samples with a measurable titer. Representative data from 1 of 2 experiments shown. A 2-way unmatched ANOVA with a Bonferroni post-test was used to determine the significance of the data between groups, which is denoted by asterisks; * P
    Igg Subclasses, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg subclasses/product/SouthernBiotech
    Average 93 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    igg subclasses - by Bioz Stars, 2020-07
    93/100 stars
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    85
    SouthernBiotech murine igg subclass standards
    Functional attributes of <t>anti-PPS14</t> <t>IgG</t> purified from serum samples collected at 25 days after secondary immunization. For each immunization group, IgG was purified from five of the 25-day postsecondary serum samples used for the isotype analysis described
    Murine Igg Subclass Standards, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murine igg subclass standards/product/SouthernBiotech
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    murine igg subclass standards - by Bioz Stars, 2020-07
    85/100 stars
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    Image Search Results


    ELISA titers in mice immunized with Pfs230C1. ELISA data for individual mice (circles) and medians (bar) are shown. Total anti-Pfs230C1 antibody levels are presented on a log scale of ELISA units (A), and IgG subclass results are given as the optical density at 405 nm (OD 405 ) (B). Dotted lines indicate the background signals.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: N-Terminal Pfs230 Domain Produced in Baculovirus as a Biological Active Transmission-Blocking Vaccine Candidate

    doi: 10.1128/CVI.00140-17

    Figure Lengend Snippet: ELISA titers in mice immunized with Pfs230C1. ELISA data for individual mice (circles) and medians (bar) are shown. Total anti-Pfs230C1 antibody levels are presented on a log scale of ELISA units (A), and IgG subclass results are given as the optical density at 405 nm (OD 405 ) (B). Dotted lines indicate the background signals.

    Article Snippet: The IgG subclass ELISA was performed with small modifications; all serum samples were diluted to 1 total IgG ELISA unit and then tested with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Bio-Rad).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay

    Aire −/− mice have an increased specific response against TI-II antigen. ( A ) Serum levels of TNP-specific IgM and IgG3 on day 7 after immunization of Aire −/− mice and wt littermate controls with TNP-Ficoll, determined by

    Journal:

    Article Title: AIRE regulates T-cell-independent B-cell responses through BAFF

    doi: 10.1073/pnas.0808205105

    Figure Lengend Snippet: Aire −/− mice have an increased specific response against TI-II antigen. ( A ) Serum levels of TNP-specific IgM and IgG3 on day 7 after immunization of Aire −/− mice and wt littermate controls with TNP-Ficoll, determined by

    Article Snippet: The 4-(hydroxy-3-nitro-phenyl) acetyl (NP)-specific antibodies were detected with alkaline phosphatase (AP)-conjugated goat anti-mouse IgM and IgG subclass antibodies (Southern Biotechnology Associates) at OD405 .

    Techniques: Mouse Assay

    Vaccine elicited anti-DIII antibodies . Total IgG titers were measured by ELISA on WNV DIII-coated plates from mice vaccinated ID or IM with DNA plasmids encoding sections of the WNV E gene, with or without molecular adjutant P28 on week 8. Each dot represents an individual mouse. Undetectable antibody titers were arbitrarily assigned a titer of 1. Error bars denote the standard error within the samples with a measurable titer. Representative data from 1 of 2 experiments shown. A 2-way unmatched ANOVA with a Bonferroni post-test was used to determine the significance of the data between groups, which is denoted by asterisks; * P

    Journal: Virology Journal

    Article Title: Enhancement of anti-DIII antibodies by the C3d derivative P28 results in lower viral titers and augments protection in mice

    doi: 10.1186/1743-422X-7-95

    Figure Lengend Snippet: Vaccine elicited anti-DIII antibodies . Total IgG titers were measured by ELISA on WNV DIII-coated plates from mice vaccinated ID or IM with DNA plasmids encoding sections of the WNV E gene, with or without molecular adjutant P28 on week 8. Each dot represents an individual mouse. Undetectable antibody titers were arbitrarily assigned a titer of 1. Error bars denote the standard error within the samples with a measurable titer. Representative data from 1 of 2 experiments shown. A 2-way unmatched ANOVA with a Bonferroni post-test was used to determine the significance of the data between groups, which is denoted by asterisks; * P

    Article Snippet: Following serial washes with PBS Tween-20 (0.05%), samples were incubated (25°C for 1 hr) with HRP conjugated goat anti-mouse IgG (1:5000) or one of four IgG subclasses (IgG1 , IgG2a , IgG2b , or IgG3 ) (Southern Biotechnology, Birmingham, AL) diluted in PBS Tween-20 (0.05%) and nonfat dry milk (5%).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay

    Functional attributes of anti-PPS14 IgG purified from serum samples collected at 25 days after secondary immunization. For each immunization group, IgG was purified from five of the 25-day postsecondary serum samples used for the isotype analysis described

    Journal:

    Article Title: Depletion of Complement Has Distinct Effects on the Primary and Secondary Antibody Responses to a Conjugate of Pneumococcal Serotype 14 Capsular Polysaccharide and a T-Cell-Dependent Protein Carrier

    doi: 10.1128/IAI.73.1.277-286.2005

    Figure Lengend Snippet: Functional attributes of anti-PPS14 IgG purified from serum samples collected at 25 days after secondary immunization. For each immunization group, IgG was purified from five of the 25-day postsecondary serum samples used for the isotype analysis described

    Article Snippet: The IgG and IgA subclass compositions of serum antibodies to PPS14 were determined by ELISA with the SBA clonotyping system/AP and murine IgG subclass standards (Southern Biotechnology Associates, Birmingham, Ala.).

    Techniques: Functional Assay, Purification