igg2c secondary antibodies  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Goat Anti Mouse IgG Fcγ Fragment Specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of mouse IgG heavy chain but not with the Fab portion of mouse immunoglobulins No antibody was detected against mouse IgM or against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    115-065-008
    Price:
    134
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    2 0
    Buy from Supplier


    Structured Review

    Jackson Immuno igg2c secondary antibodies
    Biotin SP AffiniPure Goat Anti Mouse IgG Fcγ Fragment Specific
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of mouse IgG heavy chain but not with the Fab portion of mouse immunoglobulins No antibody was detected against mouse IgM or against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/igg2c secondary antibodies/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg2c secondary antibodies - by Bioz Stars, 2020-03
    99/100 stars

    Images

    1) Product Images from "T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and non-virion association *"

    Article Title: T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and non-virion association *

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1400663

    Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.
    Figure Legend Snippet: Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.

    Techniques Used: Purification, Mouse Assay, Nickel Column, Concentration Assay, Derivative Assay, Titration

    Related Articles

    Flow Cytometry:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: Paragraph title: Flow Cytometry Analysis ... After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch).

    Amplification:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch). .. Live cells (∼60%) were sorted by propidium iodide exclusion and analyzed on a FACSCalibur machine (BD Biosciences).

    Stable Transfection:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: Colorectal HT29 cells expressing endogenous Hh and COS cells stably transfected with human Shh (Shh-COS) were detached from plates using 1 m m EDTA in PBS at 37 °C. .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch).

    Cytometry:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: Paragraph title: Flow Cytometry Analysis ... After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch).

    Labeling:

    Article Title: Single-cell analysis and sorting using droplet-based microfluidics
    Article Snippet: .. 9E10 mouse hybridoma cell line (ATCC, cat. no. CRL-1729) K562 human chronic myelogenous leukemia cell line (ATCC, cat. no. CCL-243) DMEM (Mediatech, cat. no. 10–013-CV) FBS (Life Technologies, cat. no. 10313-039) Penicillin-streptomycin (Life Technologies, cat. no. 15140-122) Streptavidin-coated beads, 6 µm (Spherotech, cat. no. SVP-60-5) Capture antibody: biotinylated whole goat IgG directed against the Fc fragment of mouse IgG (Jackson ImmunoResearch Laboratories, cat. no. 115-065-008) Detection antibody: whole goat IgG directed against the F(ab)2 fragment of mouse IgG, labeled with dylight 488 (Jackson ImmunoResearch Laboratories, cat. no. 115–485-006) Purified anti-MYC mouse monoclonal antibody (Covance, cat. no. MMS-150P) Orange CMRA (9′-(4-(and 5)-chloromethyl-2-carboxyphenyl)-7′-chloro-6′-oxo-1,2,2,4-tetramethyl-1,2-dihydropyrido[2′,3′-6]xanthene; Invitrogen, CellTracker Orange CMRA ex/em 548/576 nm, cat. no. ) Magnesium chloride hexahydrate (MgCl2 ·6H2 O; Sigma-Aldrich, cat. no. M-2670) PBS (Lifetech, cat. no. 10010-023) DNase I (Fermentas, cat. no. EN0521) Trypsin (Sigma-Aldrich, cat. no. T4549) OptiPrep density gradient medium (Sigma-Aldrich, cat. no. D1556) Tween 20 (Sigma-Aldrich, cat. no. P9416) Triton X-100 (Sigma-Aldrich, cat. no. T8787) Milli-Q ultrapurified water .. Optics table or bread board Optics posts and bases Beam-steering mirrors with kinematic mounts (Thorlabs, cat. no. KCB1 and NB1-J10) z axis translation mount (Thorlabs, cat. no. SM1Z) Rotational mount (Thorlabs, cat. no. SM1V05) XY translation mount (Thorlabs, cat. no. LM1XY) Dichroic filter mounts (Thorlabs, cat. no. C4W, FFM1, B3C and B6C) Cage rods (Thorlabs, cat. no. ER6) Lens tube caps (Thorlabs, cat. no. SM1CP2) Shutters (Thorlabs, cat. no. SM1SH1) PMT-compatible power supply

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch). .. Murine 5E1 and ch5E1 labeling of Shh-COS cells was directly detected with 15 μg/ml fluorescein isothiocyanate-conjugated goat anti-mouse Fc (catalog no. 109-096-098, Jackson ImmunoResearch) or anti-human Fc (catalog no. 115-096-071, Jackson ImmunoResearch), respectively, and cells were fixed in 2% paraformaldehyde prior to analysis.

    Purification:

    Article Title: Obesity induces pro-inflammatory B cells and impairs B cell function in old mice
    Article Snippet: To measure IgG subclasses (IgG1/G2a/G2b/G2c/G3) in lysates of VAT lymphocytes, goat anti-mouse Southern Biotech purified anti-subclass antibodies were used for coating, at the concentration of 2 μg/ml. .. Detection antibody was a biotinylated goat anti-mouse IgG antibody (Jackson ImmunoResearch Labs 115-065-071), followed by streptavidin-HRP.

    Article Title: Single-cell analysis and sorting using droplet-based microfluidics
    Article Snippet: .. 9E10 mouse hybridoma cell line (ATCC, cat. no. CRL-1729) K562 human chronic myelogenous leukemia cell line (ATCC, cat. no. CCL-243) DMEM (Mediatech, cat. no. 10–013-CV) FBS (Life Technologies, cat. no. 10313-039) Penicillin-streptomycin (Life Technologies, cat. no. 15140-122) Streptavidin-coated beads, 6 µm (Spherotech, cat. no. SVP-60-5) Capture antibody: biotinylated whole goat IgG directed against the Fc fragment of mouse IgG (Jackson ImmunoResearch Laboratories, cat. no. 115-065-008) Detection antibody: whole goat IgG directed against the F(ab)2 fragment of mouse IgG, labeled with dylight 488 (Jackson ImmunoResearch Laboratories, cat. no. 115–485-006) Purified anti-MYC mouse monoclonal antibody (Covance, cat. no. MMS-150P) Orange CMRA (9′-(4-(and 5)-chloromethyl-2-carboxyphenyl)-7′-chloro-6′-oxo-1,2,2,4-tetramethyl-1,2-dihydropyrido[2′,3′-6]xanthene; Invitrogen, CellTracker Orange CMRA ex/em 548/576 nm, cat. no. ) Magnesium chloride hexahydrate (MgCl2 ·6H2 O; Sigma-Aldrich, cat. no. M-2670) PBS (Lifetech, cat. no. 10010-023) DNase I (Fermentas, cat. no. EN0521) Trypsin (Sigma-Aldrich, cat. no. T4549) OptiPrep density gradient medium (Sigma-Aldrich, cat. no. D1556) Tween 20 (Sigma-Aldrich, cat. no. P9416) Triton X-100 (Sigma-Aldrich, cat. no. T8787) Milli-Q ultrapurified water .. Optics table or bread board Optics posts and bases Beam-steering mirrors with kinematic mounts (Thorlabs, cat. no. KCB1 and NB1-J10) z axis translation mount (Thorlabs, cat. no. SM1Z) Rotational mount (Thorlabs, cat. no. SM1V05) XY translation mount (Thorlabs, cat. no. LM1XY) Dichroic filter mounts (Thorlabs, cat. no. C4W, FFM1, B3C and B6C) Cage rods (Thorlabs, cat. no. ER6) Lens tube caps (Thorlabs, cat. no. SM1CP2) Shutters (Thorlabs, cat. no. SM1SH1) PMT-compatible power supply

    Enzyme-linked Immunosorbent Assay:

    Article Title: Obesity induces pro-inflammatory B cells and impairs B cell function in old mice
    Article Snippet: Paragraph title: 2.11. Enzyme-linked immunosorbent assay (ELISA) ... Detection antibody was a biotinylated goat anti-mouse IgG antibody (Jackson ImmunoResearch Labs 115-065-071), followed by streptavidin-HRP.

    Concentration Assay:

    Article Title: Obesity induces pro-inflammatory B cells and impairs B cell function in old mice
    Article Snippet: To measure IgG subclasses (IgG1/G2a/G2b/G2c/G3) in lysates of VAT lymphocytes, goat anti-mouse Southern Biotech purified anti-subclass antibodies were used for coating, at the concentration of 2 μg/ml. .. Detection antibody was a biotinylated goat anti-mouse IgG antibody (Jackson ImmunoResearch Labs 115-065-071), followed by streptavidin-HRP.

    Incubation:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: After washing in growth media followed by PBS, cells were incubated in triplicate at the indicated concentrations of m5E1 or ch5E1 for 35 min in FACS buffer (3% fetal bovine serum in PBS; fetal bovine serum contains ∼3 m m Ca2+ ( )). .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch).

    Transfection:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: Colorectal HT29 cells expressing endogenous Hh and COS cells stably transfected with human Shh (Shh-COS) were detached from plates using 1 m m EDTA in PBS at 37 °C. .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch).

    Expressing:

    Article Title: T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and non-virion association *
    Article Snippet: Biotinylated goat anti-mouse IgG, IgG1 and IgG2c secondary antibodies were purchased from Jackson ImmunoResearch (Cat# 115-065-008, 115-065-205, and 115-065-208, respectively). .. For monitoring protein expression on arrays, monoclonal mouse anti-polyhistidine (‘HIS’; clone His-1, from Sigma Cat # H1029) and rat anti-hemagglutinin (‘HA’; clone 3F10, anti-HA high affinity, Roche Cat# 11 867 423 001) were used.

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: Colorectal HT29 cells expressing endogenous Hh and COS cells stably transfected with human Shh (Shh-COS) were detached from plates using 1 m m EDTA in PBS at 37 °C. .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch).

    FACS:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch). .. Live cells (∼60%) were sorted by propidium iodide exclusion and analyzed on a FACSCalibur machine (BD Biosciences).

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  • 99
    Jackson Immuno peroxidase affinipure goat anti mouse igg fcγ subclass 2c specific
    ELISA quantification of naïve serum immunoglobulin. Concentration of total IgM, IgG1, IgG2b, <t>IgG2c,</t> and IgG3 immunoglobulin isotypes from serum of naïve WT, Cr1KO , and Cr1/2KO mice. (n=11; 8–16 week old sex matched mice; error bars represent SEM; *=p
    Peroxidase Affinipure Goat Anti Mouse Igg Fcγ Subclass 2c Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase affinipure goat anti mouse igg fcγ subclass 2c specific/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    peroxidase affinipure goat anti mouse igg fcγ subclass 2c specific - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno igg2c secondary antibodies
    Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and <t>IgG2c</t> and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.
    Igg2c Secondary Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2c secondary antibodies/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg2c secondary antibodies - by Bioz Stars, 2020-03
    99/100 stars
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    99
    Jackson Immuno igg
    Disrupted lipid homeostasis and immunity in Abcg1 T2-KO mice. A–E: BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice was diluted 1:5 to 1:250 and tested for binding to <t>IgG</t> (A), IgA (B), IgG2c (C), <t>IgG1</t> (D), and IgM (E). ALP-conjugated antibodies were used for detection. Data are presented as mean antibody titer (ng/ml) ± SEM (n = 5–10 mice/genotype). F–H: Cholesterol, cholesteryl ester, and PC and their derivatives were quantified by ESI-MS/MS in BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice. Data are presented as mean lipid level (pmol/μl) ± SEM (n = 3–6 mice/genotype). Significance was measured by Student’s t -test. * P
    Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg/product/Jackson Immuno
    Average 99 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
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    94
    Jackson Immuno biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific
    Disrupted lipid homeostasis and immunity in Abcg1 T2-KO mice. A–E: BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice was diluted 1:5 to 1:250 and tested for binding to <t>IgG</t> (A), IgA (B), IgG2c (C), <t>IgG1</t> (D), and IgM (E). ALP-conjugated antibodies were used for detection. Data are presented as mean antibody titer (ng/ml) ± SEM (n = 5–10 mice/genotype). F–H: Cholesterol, cholesteryl ester, and PC and their derivatives were quantified by ESI-MS/MS in BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice. Data are presented as mean lipid level (pmol/μl) ± SEM (n = 3–6 mice/genotype). Significance was measured by Student’s t -test. * P
    Biotin Sp Affinipure Goat Anti Mouse Igg Fcγ Subclass 2c Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
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    Image Search Results


    ELISA quantification of naïve serum immunoglobulin. Concentration of total IgM, IgG1, IgG2b, IgG2c, and IgG3 immunoglobulin isotypes from serum of naïve WT, Cr1KO , and Cr1/2KO mice. (n=11; 8–16 week old sex matched mice; error bars represent SEM; *=p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Optimal Germinal Center B cell Activation and T-dependent Antibody Responses Require the Expression of the Mouse Complement Receptor Cr1 *

    doi: 10.4049/jimmunol.1203176

    Figure Lengend Snippet: ELISA quantification of naïve serum immunoglobulin. Concentration of total IgM, IgG1, IgG2b, IgG2c, and IgG3 immunoglobulin isotypes from serum of naïve WT, Cr1KO , and Cr1/2KO mice. (n=11; 8–16 week old sex matched mice; error bars represent SEM; *=p

    Article Snippet: All secondary antibodies were conjugated to horseradish peroxidase: rabbit anti-mouse IgM (Jackson ImmunoResearch #315-035-049), rabbit anti-mouse IgG1 (Zymed #61-0120), rabbit anti-mouse IgG2b (Zymed #61-0320), goat anti-mouse IgG2c (Jackson ImmunoResearch #115-035-208), and goat anti-mouse IgG3 (AbD Serotec #STAR6P).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Mouse Assay

    TI-1, TI-2, and TD antigen specific immunoglobulin response. A , Deviation from WT in production of antigen specific immunoglobulin of the isotypes IgM, IgG1, IgG2b, IgG2c and IgG3 by Cr1KO and Cr1/2KO ) B , ELISA analysis of KLH specific IgM and IgG from TNP-KLH (100µg) immunized mice shown in panel A (n=10; 8–12 week old sex matched mice). C , ELISA analysis of SRBC specific IgM and IgG from mice immunized i.v. with 1×10 8 SRBC on days 0 and 21 (n=6; sex matched 8–10 week old mice). (One-way ANOVA performed for each day and Tukey’s Multiple Comparison post test results shown for all time points found to be at least p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Optimal Germinal Center B cell Activation and T-dependent Antibody Responses Require the Expression of the Mouse Complement Receptor Cr1 *

    doi: 10.4049/jimmunol.1203176

    Figure Lengend Snippet: TI-1, TI-2, and TD antigen specific immunoglobulin response. A , Deviation from WT in production of antigen specific immunoglobulin of the isotypes IgM, IgG1, IgG2b, IgG2c and IgG3 by Cr1KO and Cr1/2KO ) B , ELISA analysis of KLH specific IgM and IgG from TNP-KLH (100µg) immunized mice shown in panel A (n=10; 8–12 week old sex matched mice). C , ELISA analysis of SRBC specific IgM and IgG from mice immunized i.v. with 1×10 8 SRBC on days 0 and 21 (n=6; sex matched 8–10 week old mice). (One-way ANOVA performed for each day and Tukey’s Multiple Comparison post test results shown for all time points found to be at least p

    Article Snippet: All secondary antibodies were conjugated to horseradish peroxidase: rabbit anti-mouse IgM (Jackson ImmunoResearch #315-035-049), rabbit anti-mouse IgG1 (Zymed #61-0120), rabbit anti-mouse IgG2b (Zymed #61-0320), goat anti-mouse IgG2c (Jackson ImmunoResearch #115-035-208), and goat anti-mouse IgG3 (AbD Serotec #STAR6P).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay

    Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and non-virion association *

    doi: 10.4049/jimmunol.1400663

    Figure Lengend Snippet: Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.

    Article Snippet: Biotinylated goat anti-mouse IgG, IgG1 and IgG2c secondary antibodies were purchased from Jackson ImmunoResearch (Cat# 115-065-008, 115-065-205, and 115-065-208, respectively).

    Techniques: Purification, Mouse Assay, Nickel Column, Concentration Assay, Derivative Assay, Titration

    Disrupted lipid homeostasis and immunity in Abcg1 T2-KO mice. A–E: BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice was diluted 1:5 to 1:250 and tested for binding to IgG (A), IgA (B), IgG2c (C), IgG1 (D), and IgM (E). ALP-conjugated antibodies were used for detection. Data are presented as mean antibody titer (ng/ml) ± SEM (n = 5–10 mice/genotype). F–H: Cholesterol, cholesteryl ester, and PC and their derivatives were quantified by ESI-MS/MS in BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice. Data are presented as mean lipid level (pmol/μl) ± SEM (n = 3–6 mice/genotype). Significance was measured by Student’s t -test. * P

    Journal: Journal of Lipid Research

    Article Title: ABCG1 regulates pulmonary surfactant metabolism in mice and men [S]

    doi: 10.1194/jlr.M075101

    Figure Lengend Snippet: Disrupted lipid homeostasis and immunity in Abcg1 T2-KO mice. A–E: BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice was diluted 1:5 to 1:250 and tested for binding to IgG (A), IgA (B), IgG2c (C), IgG1 (D), and IgM (E). ALP-conjugated antibodies were used for detection. Data are presented as mean antibody titer (ng/ml) ± SEM (n = 5–10 mice/genotype). F–H: Cholesterol, cholesteryl ester, and PC and their derivatives were quantified by ESI-MS/MS in BAL fluid from Abcg1 flox/flox and Abcg1 T2-KO mice. Data are presented as mean lipid level (pmol/μl) ± SEM (n = 3–6 mice/genotype). Significance was measured by Student’s t -test. * P

    Article Snippet: In brief, capture antigens were coated on plates at 5 μg/ml in PBS overnight at 4°C [IgM (1:100 goat anti-mouse IgM; Sigma-Aldrich), IgG (1:400 goat anti-mouse IgG; Pierce Protein Biology), IgG1 (1:250 goat anti-mouse IgG1; Jackson ImmunoResearch Laboratories), IgG (1:250 goat anti-mouse IgG2c; Jackson ImmunoResearch Laboratories), IgA (1:100 goat anti-mouse IgA; Sigma-Aldrich)].

    Techniques: Mouse Assay, Binding Assay, ALP Assay, Mass Spectrometry