igg2c hrp  (SouthernBiotech)

 
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    Name:
    Goat Anti Mouse IgG2c HRP
    Description:

    Catalog Number:
    1078-05
    Price:
    None
    Source:
    Pooled antisera from goats hyperimmunized with mouse IgG2c
    Applications:
    Quality tested applications for relevant formats include -ELISAFLISA
    Format:
    HRP (Horseradish Peroxidase)
    Isotype:
    Goat IgG
    Buy from Supplier


    Structured Review

    SouthernBiotech igg2c hrp
    Goat Anti Mouse IgG2c HRP

    https://www.bioz.com/result/igg2c hrp/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg2c hrp - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "An alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates"

    Article Title: An alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates

    Journal: Science Translational Medicine

    doi: 10.1126/scitranslmed.abc9396

    The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and IgG2c antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p
    Figure Legend Snippet: The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and IgG2c antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Enzyme-linked Immunospot

    2) Product Images from "Vaccine nanoparticles displaying recombinant Ebola virus glycoprotein for induction of potent antibody and polyfunctional T cell responses."

    Article Title: Vaccine nanoparticles displaying recombinant Ebola virus glycoprotein for induction of potent antibody and polyfunctional T cell responses.

    Journal: Nanomedicine : nanotechnology, biology, and medicine

    doi: 10.1016/j.nano.2018.11.005

    Analyses of immune sera after vaccination. (A) Mice (n = 10/group) were vaccinated subcutaneously at the tail base with either blank ICMV + MPLA, rGP, rGP + MPLA, rGP ICMV + MPLA, or rGP NTA ICMV + MPLA on day 0 and 21. Antigen and adjuvant doses were 3 µg rGP and 2.5 µg MPLA, respectively, for both injections. Serum was collected two weeks after final vaccination. (B-E) EBOV GP-specific IgM, total IgG, IgG1, or IgG2c antibody responses were measured by ELISA. Dotted and dashed lines represent minimum and maximum dilutions tested, respectively. Measurements reported as geometric mean ± SEM. Non-seroconverted serum samples were assigned log-values of 0.5 (IgM) or 1.5 (IgG and subclasses) for graphical representation and statistical analysis. (F-I) Box and whisker plots of rVSV-GP neutralization by diluted serum from five randomly selected mice from each group. Percent neutralization was determined by residual infectivity of sample groups compared to naïve serum. (B-I) Statistical analysis performed by one-way ANOVA, followed by Tukey’s multiple comparisons test. * p
    Figure Legend Snippet: Analyses of immune sera after vaccination. (A) Mice (n = 10/group) were vaccinated subcutaneously at the tail base with either blank ICMV + MPLA, rGP, rGP + MPLA, rGP ICMV + MPLA, or rGP NTA ICMV + MPLA on day 0 and 21. Antigen and adjuvant doses were 3 µg rGP and 2.5 µg MPLA, respectively, for both injections. Serum was collected two weeks after final vaccination. (B-E) EBOV GP-specific IgM, total IgG, IgG1, or IgG2c antibody responses were measured by ELISA. Dotted and dashed lines represent minimum and maximum dilutions tested, respectively. Measurements reported as geometric mean ± SEM. Non-seroconverted serum samples were assigned log-values of 0.5 (IgM) or 1.5 (IgG and subclasses) for graphical representation and statistical analysis. (F-I) Box and whisker plots of rVSV-GP neutralization by diluted serum from five randomly selected mice from each group. Percent neutralization was determined by residual infectivity of sample groups compared to naïve serum. (B-I) Statistical analysis performed by one-way ANOVA, followed by Tukey’s multiple comparisons test. * p

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Whisker Assay, Neutralization, Infection

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: The impact of size on particle drainage dynamics and antibody response
    Article Snippet: .. IgG subclasses were measured from day 21 sera using the same ELISA protocol with the following secondary Abs: goat anti-mouse IgG1-HRP (BD Biosciences, San Jose, California), goat anti-mouse IgG2b-HRP (1:1000) (Thermo Fischer Scientific, Waltham, Massachusetts), goat anti-mouse IgG2c-HRP (Southern Biotech, Birmingham, Alabama) 1:4000, rat anti-mouse IgG3-biotin (Becton, Dickinson, Franklin Lakes, New Jersey) 1:2000 followed by streptavidin-HRP (Dako, Glostrup, Denmark) 1:1000 incubated at 37°C for 1h. ..

    Article Title: The Transcription Factor T-Bet Is Required for Optimal Type I Follicular Helper T Cell Maintenance During Acute Viral Infection
    Article Snippet: The antibodies and reagents used in flow cytometry staining are listed in . .. Enzyme-Linked Immunosorbent Assay LCMV-specific IgG and IgG2c were titrated with LCMV lysates and the secondary antibodies HRP-conjugated goat anti-mouse IgG (1036-05; SouthernBiotech) and HRP-conjugated goat anti-mouse IgG2c (1078-05; SouthernBiotech) as previously described ( ). .. Adoptive Cell Transfer To examine the LCMV-specific TFH cell response, 1 × 106 (for analysis before day 3 or after day 30) or 2 × 105 (for analysis between day 3 and day 30) sorted naïve or retrovirus-transduced CD45.1+ SMARTA cells (WT or Tbx21−/− ) were adoptively transferred into naïve or infection-matched CD45.2+ mice (WT or Tbx21−/− ) according to the requirements of the experiments.

    Incubation:

    Article Title: The impact of size on particle drainage dynamics and antibody response
    Article Snippet: .. IgG subclasses were measured from day 21 sera using the same ELISA protocol with the following secondary Abs: goat anti-mouse IgG1-HRP (BD Biosciences, San Jose, California), goat anti-mouse IgG2b-HRP (1:1000) (Thermo Fischer Scientific, Waltham, Massachusetts), goat anti-mouse IgG2c-HRP (Southern Biotech, Birmingham, Alabama) 1:4000, rat anti-mouse IgG3-biotin (Becton, Dickinson, Franklin Lakes, New Jersey) 1:2000 followed by streptavidin-HRP (Dako, Glostrup, Denmark) 1:1000 incubated at 37°C for 1h. ..

    Article Title: N6-methyladenosine modification controls circular RNA immunity
    Article Snippet: Serum samples from immunized mice at the indicated time points were serially diluted in 0.1% BSA in PBS/0.5% Tween-20 and incubated on blocked plates for 2 h at room temperature. .. Wells were washed and incubated with 1:5000 anti-mouse IgG-HRP (Southern Biotech, 1036–05), 1:5000 anti-mouse IgG1 –HRP conjugate (Southern Biotech, 1071–05) and 1:2000 anti-mouse IgG2c–HRP conjugate (Southern Biotech, 1078–05) in PBS/0.5% Tween-20 for 2 h at room temperature. .. Plates were washed and developed using 100 µl per well of tetramethylbenzidine (TMB) substrate (Thermo Fisher Scientific, 34028), followed by stopping the reaction using stop solution (Thermo Fisher Scientific, N600).

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    SouthernBiotech igg2c hrp
    The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and <t>IgG2c</t> antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p
    Igg2c Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2c hrp/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg2c hrp - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    94
    SouthernBiotech igg1
    Binding of antigen-specific <t>IgG</t> subclasses to human glomerular and lung tissue. Representative Western blot analyses of the antigen-specific IgG subclasses binding to lung tissue extracts (LTE) and human glomerular extracts (HGE). (A) Serum from patient 1 was positive for all THSD7A-specific IgG subclasses. (B) Serum from patient 2 showed detectability for THSD7A-specific <t>IgG3</t> and <t>IgG4.</t> (C) Serum from patient 3 was positive for PLA 2 R1-specific IgG3 and IgG4.
    Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg1/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg1 - by Bioz Stars, 2021-06
    94/100 stars
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    95
    SouthernBiotech goat anti mouse igg2c hrp
    The effect of Diluvac Forte® on IgG, IgG1 and <t>IgG2c</t> titers after DNA and protein immunization. Mice were vaccinated twice (on day 0 and 21) i.d. with 1 µg protein or 50 µg DNA with or without Diluvac (n = 4 in each group). The levels of IgG (A), IgG1 (B) and IgG2c (C) in the sera at day 35 were measured by ELISA against the recombinant influenza H1pdm09 protein. The antibody titers are expressed as the reciprocal of the sample dilution giving an optical density (OD) value of 1.0. The error bars indicate mean ± SEM. If the groups differ significantly (p
    Goat Anti Mouse Igg2c Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg2c hrp/product/SouthernBiotech
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg2c hrp - by Bioz Stars, 2021-06
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    95
    SouthernBiotech igg3
    Anti-spike RBD antibody levels correlated with memory B cell frequency in most seropositive convalescent plasma donors. (A-F) Correlation of area under the curve for anti-RBD plasma absorbance vs. memory B cell frequency from convalescent subjects seropositive for each isotype (IgG1 <t>IgG3</t> n = 15, IgM n = 16). Subjects whose data points are circled in (A) were isolated from apparent outliers for statistical analysis of IgG1 in (B, G, and J; n = 12). (G-I) Scatterplot correlation of area under the curve for anti-RBD plasma absorbance vs. IgM + memory B cell frequency among viable CD19 + lymphocytes. (J-L) Correlation of area under the curve for anti-RBD plasma absorbance vs. switched memory B cell frequency among viable CD19 + lymphocytes. Spearman’s correlation r s value and 95% confidence intervals shown with two-tailed p value, alpha = 0.05 for all analyses. Regression lines shown to demonstrate trend only. Sample number distribution was the same between total, IgM + and switched memory B cell analyses.
    Igg3, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg3/product/SouthernBiotech
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    Image Search Results


    The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and IgG2c antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p

    Journal: Science Translational Medicine

    Article Title: An alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates

    doi: 10.1126/scitranslmed.abc9396

    Figure Lengend Snippet: The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and IgG2c antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p

    Article Snippet: Then, in consecutive order, washes in PBS/Tween, serially diluted serum samples, anti-mouse or-monkey IgG, IgG1, IgG2a, or IgG2c-HRP (Southern Biotech) and TMB then HCL were added to the plates.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Enzyme-linked Immunospot

    Binding of antigen-specific IgG subclasses to human glomerular and lung tissue. Representative Western blot analyses of the antigen-specific IgG subclasses binding to lung tissue extracts (LTE) and human glomerular extracts (HGE). (A) Serum from patient 1 was positive for all THSD7A-specific IgG subclasses. (B) Serum from patient 2 showed detectability for THSD7A-specific IgG3 and IgG4. (C) Serum from patient 3 was positive for PLA 2 R1-specific IgG3 and IgG4.

    Journal: Frontiers in Immunology

    Article Title: Antigen-Specific IgG Subclasses in Primary and Malignancy-Associated Membranous Nephropathy

    doi: 10.3389/fimmu.2018.03035

    Figure Lengend Snippet: Binding of antigen-specific IgG subclasses to human glomerular and lung tissue. Representative Western blot analyses of the antigen-specific IgG subclasses binding to lung tissue extracts (LTE) and human glomerular extracts (HGE). (A) Serum from patient 1 was positive for all THSD7A-specific IgG subclasses. (B) Serum from patient 2 showed detectability for THSD7A-specific IgG3 and IgG4. (C) Serum from patient 3 was positive for PLA 2 R1-specific IgG3 and IgG4.

    Article Snippet: For detection of subclass-specific IgG in Western blot analyses, horseradish peroxidase-conjugated monoclonal anti-human IgG1 (9054-05), IgG2 (9060-05), IgG3 (9210-05), IgG4 (9200-05), and IgG (9040-05) antibodies from mouse were used (IgG1, IgG2, and IgG3 at a 1:5,000 dilution; IgG4 at a 1:10,000 dilution; IgG at a 1:20,000 dilution, all SouthernBiotech, Birmingham, USA), diluted in 5% dry milk in PBS-T.

    Techniques: Binding Assay, Western Blot, Proximity Ligation Assay

    Representative Western blots of patients with primary MN and malignancy-associated MN. Representative Western blot analyses of patients with all 4 subclasses of antigen-specific IgG binding. (A) Patient 1 with PLA 2 R1-associated MN and malignancy. (B) Patient 2 with PLA 2 R1-associated MN and no malignancy. (C) Patient 3 with THSD7A-associated MN and malignancy. (D) Patient 4 with THSD7A-associated MN and no malignancy.

    Journal: Frontiers in Immunology

    Article Title: Antigen-Specific IgG Subclasses in Primary and Malignancy-Associated Membranous Nephropathy

    doi: 10.3389/fimmu.2018.03035

    Figure Lengend Snippet: Representative Western blots of patients with primary MN and malignancy-associated MN. Representative Western blot analyses of patients with all 4 subclasses of antigen-specific IgG binding. (A) Patient 1 with PLA 2 R1-associated MN and malignancy. (B) Patient 2 with PLA 2 R1-associated MN and no malignancy. (C) Patient 3 with THSD7A-associated MN and malignancy. (D) Patient 4 with THSD7A-associated MN and no malignancy.

    Article Snippet: For detection of subclass-specific IgG in Western blot analyses, horseradish peroxidase-conjugated monoclonal anti-human IgG1 (9054-05), IgG2 (9060-05), IgG3 (9210-05), IgG4 (9200-05), and IgG (9040-05) antibodies from mouse were used (IgG1, IgG2, and IgG3 at a 1:5,000 dilution; IgG4 at a 1:10,000 dilution; IgG at a 1:20,000 dilution, all SouthernBiotech, Birmingham, USA), diluted in 5% dry milk in PBS-T.

    Techniques: Western Blot, Binding Assay, Proximity Ligation Assay

    Antigen-specific IgG subclasses during follow-up and relapse. The PLA 2 R1- (A) and THSD7A-specific (B) IgG subclasses did not significantly change during the follow-up period. At the time of relapse of PLA 2 R1-ab, antigen-specific IgG subclasses were similar to the distribution observed at baseline (C) .

    Journal: Frontiers in Immunology

    Article Title: Antigen-Specific IgG Subclasses in Primary and Malignancy-Associated Membranous Nephropathy

    doi: 10.3389/fimmu.2018.03035

    Figure Lengend Snippet: Antigen-specific IgG subclasses during follow-up and relapse. The PLA 2 R1- (A) and THSD7A-specific (B) IgG subclasses did not significantly change during the follow-up period. At the time of relapse of PLA 2 R1-ab, antigen-specific IgG subclasses were similar to the distribution observed at baseline (C) .

    Article Snippet: For detection of subclass-specific IgG in Western blot analyses, horseradish peroxidase-conjugated monoclonal anti-human IgG1 (9054-05), IgG2 (9060-05), IgG3 (9210-05), IgG4 (9200-05), and IgG (9040-05) antibodies from mouse were used (IgG1, IgG2, and IgG3 at a 1:5,000 dilution; IgG4 at a 1:10,000 dilution; IgG at a 1:20,000 dilution, all SouthernBiotech, Birmingham, USA), diluted in 5% dry milk in PBS-T.

    Techniques: Proximity Ligation Assay

    THSD7A and PLA 2 R1 are differentially glycosylated in human lung and kidney. (A) The THSD7A-ab positive serum shows positive binding to the antigen of human lung tissue (LTE) and glomerular extract (HGE), however, the antigen migrates differentially in both tissues. The renal antigen showed a higher molecular weight compared to the lung antigen. (B) Upon addition of deglycosylating enzymes THSD7A shows the same migration pattern in both tissues, suggesting a polypeptide backbone with different glycosylation in the lung and the kidney. (C,D) Similar observations were made for PLA 2 R1 in lung and kidney tissue. 140 kDa band in LTE represents residual IgG, which was also detected in negative controls (healthy serum, n = 5, data not shown).

    Journal: Frontiers in Immunology

    Article Title: Antigen-Specific IgG Subclasses in Primary and Malignancy-Associated Membranous Nephropathy

    doi: 10.3389/fimmu.2018.03035

    Figure Lengend Snippet: THSD7A and PLA 2 R1 are differentially glycosylated in human lung and kidney. (A) The THSD7A-ab positive serum shows positive binding to the antigen of human lung tissue (LTE) and glomerular extract (HGE), however, the antigen migrates differentially in both tissues. The renal antigen showed a higher molecular weight compared to the lung antigen. (B) Upon addition of deglycosylating enzymes THSD7A shows the same migration pattern in both tissues, suggesting a polypeptide backbone with different glycosylation in the lung and the kidney. (C,D) Similar observations were made for PLA 2 R1 in lung and kidney tissue. 140 kDa band in LTE represents residual IgG, which was also detected in negative controls (healthy serum, n = 5, data not shown).

    Article Snippet: For detection of subclass-specific IgG in Western blot analyses, horseradish peroxidase-conjugated monoclonal anti-human IgG1 (9054-05), IgG2 (9060-05), IgG3 (9210-05), IgG4 (9200-05), and IgG (9040-05) antibodies from mouse were used (IgG1, IgG2, and IgG3 at a 1:5,000 dilution; IgG4 at a 1:10,000 dilution; IgG at a 1:20,000 dilution, all SouthernBiotech, Birmingham, USA), diluted in 5% dry milk in PBS-T.

    Techniques: Proximity Ligation Assay, Binding Assay, Molecular Weight, Migration

    Distribution of PLA 2 R1- and THSD7A-specific IgG subclasses at baseline. (A) Detection of antigen-specific IgG subclasses in the complete study cohort by Western Blot using recombinant proteins. All patients were found to be positive for antigen-specific IgG4-antibodies. Antigen-specific IgG3 antibodies were more common than antigen-specific IgG1, while IgG2 was detected less often. The distribution of (B) PLA 2 R1- and (C) THSD7A-specific IgG subclasses was not different in patients with or without malignancy. Patients with (D) PLA 2 R1- or (E) THSD7A-associated MN were grouped depending on the clinical outcome: negativity of autoantibodies during follow-up, remission of proteinuria, doubling of serum creatinine. IgG1-3 depicts the percentage of patients in whom at least one antigen-specific IgG subclass (IgG1, IgG2, or IgG3) was detected in addition to IgG4.

    Journal: Frontiers in Immunology

    Article Title: Antigen-Specific IgG Subclasses in Primary and Malignancy-Associated Membranous Nephropathy

    doi: 10.3389/fimmu.2018.03035

    Figure Lengend Snippet: Distribution of PLA 2 R1- and THSD7A-specific IgG subclasses at baseline. (A) Detection of antigen-specific IgG subclasses in the complete study cohort by Western Blot using recombinant proteins. All patients were found to be positive for antigen-specific IgG4-antibodies. Antigen-specific IgG3 antibodies were more common than antigen-specific IgG1, while IgG2 was detected less often. The distribution of (B) PLA 2 R1- and (C) THSD7A-specific IgG subclasses was not different in patients with or without malignancy. Patients with (D) PLA 2 R1- or (E) THSD7A-associated MN were grouped depending on the clinical outcome: negativity of autoantibodies during follow-up, remission of proteinuria, doubling of serum creatinine. IgG1-3 depicts the percentage of patients in whom at least one antigen-specific IgG subclass (IgG1, IgG2, or IgG3) was detected in addition to IgG4.

    Article Snippet: For detection of subclass-specific IgG in Western blot analyses, horseradish peroxidase-conjugated monoclonal anti-human IgG1 (9054-05), IgG2 (9060-05), IgG3 (9210-05), IgG4 (9200-05), and IgG (9040-05) antibodies from mouse were used (IgG1, IgG2, and IgG3 at a 1:5,000 dilution; IgG4 at a 1:10,000 dilution; IgG at a 1:20,000 dilution, all SouthernBiotech, Birmingham, USA), diluted in 5% dry milk in PBS-T.

    Techniques: Proximity Ligation Assay, Western Blot, Recombinant

    The effect of Diluvac Forte® on IgG, IgG1 and IgG2c titers after DNA and protein immunization. Mice were vaccinated twice (on day 0 and 21) i.d. with 1 µg protein or 50 µg DNA with or without Diluvac (n = 4 in each group). The levels of IgG (A), IgG1 (B) and IgG2c (C) in the sera at day 35 were measured by ELISA against the recombinant influenza H1pdm09 protein. The antibody titers are expressed as the reciprocal of the sample dilution giving an optical density (OD) value of 1.0. The error bars indicate mean ± SEM. If the groups differ significantly (p

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Increased humoral immunity by DNA vaccination using an α-tocopherol-based adjuvant

    doi: 10.1080/21645515.2017.1321183

    Figure Lengend Snippet: The effect of Diluvac Forte® on IgG, IgG1 and IgG2c titers after DNA and protein immunization. Mice were vaccinated twice (on day 0 and 21) i.d. with 1 µg protein or 50 µg DNA with or without Diluvac (n = 4 in each group). The levels of IgG (A), IgG1 (B) and IgG2c (C) in the sera at day 35 were measured by ELISA against the recombinant influenza H1pdm09 protein. The antibody titers are expressed as the reciprocal of the sample dilution giving an optical density (OD) value of 1.0. The error bars indicate mean ± SEM. If the groups differ significantly (p

    Article Snippet: For the detection of IgG1 and IgG2c, goat-anti-mouse IgG1/HRP (Southern Biotech cat. no. 1070–05) and goat-anti-mouse IgG2c/HRP (Southern Biotech cat. no. 1079–05) were used, respectively.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Recombinant

    The effect of α-tocopherol on IgG1 and IgG2c titers after DNA immunization. Serum IgG1 (A) and IgG2c (B) anti-H1pdm09 at day 35 induced by immunization of mice with DNA vaccine (50 µg DNA/mice/immunization time-point) mixed with α-tocopherol at high (150 mg/ml) medium (100 mg/ml) and low (50 mg/ml) concentrations in an emulsion, emulsion without α-tocopherol or PBS. CB6F1 mice (n = 6/group) were immunized at day 0 and 21. The antibody titers are expressed as the reciprocal of the sample dilution giving an optical density (OD) value of 1.0. The error bars indicate the mean ± SEM. If the groups differ significantly (p

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Increased humoral immunity by DNA vaccination using an α-tocopherol-based adjuvant

    doi: 10.1080/21645515.2017.1321183

    Figure Lengend Snippet: The effect of α-tocopherol on IgG1 and IgG2c titers after DNA immunization. Serum IgG1 (A) and IgG2c (B) anti-H1pdm09 at day 35 induced by immunization of mice with DNA vaccine (50 µg DNA/mice/immunization time-point) mixed with α-tocopherol at high (150 mg/ml) medium (100 mg/ml) and low (50 mg/ml) concentrations in an emulsion, emulsion without α-tocopherol or PBS. CB6F1 mice (n = 6/group) were immunized at day 0 and 21. The antibody titers are expressed as the reciprocal of the sample dilution giving an optical density (OD) value of 1.0. The error bars indicate the mean ± SEM. If the groups differ significantly (p

    Article Snippet: For the detection of IgG1 and IgG2c, goat-anti-mouse IgG1/HRP (Southern Biotech cat. no. 1070–05) and goat-anti-mouse IgG2c/HRP (Southern Biotech cat. no. 1079–05) were used, respectively.

    Techniques: Mouse Assay

    Anti-spike RBD antibody levels correlated with memory B cell frequency in most seropositive convalescent plasma donors. (A-F) Correlation of area under the curve for anti-RBD plasma absorbance vs. memory B cell frequency from convalescent subjects seropositive for each isotype (IgG1 IgG3 n = 15, IgM n = 16). Subjects whose data points are circled in (A) were isolated from apparent outliers for statistical analysis of IgG1 in (B, G, and J; n = 12). (G-I) Scatterplot correlation of area under the curve for anti-RBD plasma absorbance vs. IgM + memory B cell frequency among viable CD19 + lymphocytes. (J-L) Correlation of area under the curve for anti-RBD plasma absorbance vs. switched memory B cell frequency among viable CD19 + lymphocytes. Spearman’s correlation r s value and 95% confidence intervals shown with two-tailed p value, alpha = 0.05 for all analyses. Regression lines shown to demonstrate trend only. Sample number distribution was the same between total, IgM + and switched memory B cell analyses.

    Journal: PLoS ONE

    Article Title: Switched and unswitched memory B cells detected during SARS-CoV-2 convalescence correlate with limited symptom duration

    doi: 10.1371/journal.pone.0244855

    Figure Lengend Snippet: Anti-spike RBD antibody levels correlated with memory B cell frequency in most seropositive convalescent plasma donors. (A-F) Correlation of area under the curve for anti-RBD plasma absorbance vs. memory B cell frequency from convalescent subjects seropositive for each isotype (IgG1 IgG3 n = 15, IgM n = 16). Subjects whose data points are circled in (A) were isolated from apparent outliers for statistical analysis of IgG1 in (B, G, and J; n = 12). (G-I) Scatterplot correlation of area under the curve for anti-RBD plasma absorbance vs. IgM + memory B cell frequency among viable CD19 + lymphocytes. (J-L) Correlation of area under the curve for anti-RBD plasma absorbance vs. switched memory B cell frequency among viable CD19 + lymphocytes. Spearman’s correlation r s value and 95% confidence intervals shown with two-tailed p value, alpha = 0.05 for all analyses. Regression lines shown to demonstrate trend only. Sample number distribution was the same between total, IgM + and switched memory B cell analyses.

    Article Snippet: IgM was detected using goat-anti-human-IgM-HRP (MilliporeSigma, #A6907), and IgG subclass antibodies were detected with mouse horseradish peroxidase (HRP)-conjugated anti-human IgG1 (9054–05), IgG2 (9060–05), IgG3 (9210–05), and IgG4 (9200–05) from SouthernBiotech (Birmingham, AL, USA).

    Techniques: Isolation, Two Tailed Test