igg1 igg3 fc  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    anti Mouse IgG Fc HRP
    Description:

    Catalog Number:
    pa1-84824
    Price:
    None
    Buy from Supplier


    Structured Review

    Thermo Fisher igg1 igg3 fc
    PA and PG binding assessment of engineered antibodies by linear-gradient chromatography. (a) Overlay of HiTrap® MabSelect™ SuRe™ PA chromatograms (RT). An <t>IgG3-like</t> antibody (IgG 1133) based on the VH3 subclass (blue) still bound the MabSelect™ SuRe™ resin even though elution occurred at a mild pH (~pH 4.2). Adding the Fab substitution G65S (green) or N82aS (red) completely abolished binding (Kabat numbering). (b) Overlay of HiTrap® PG HP chromatograms (RT). An <t>IgG1</t> antibody carrying the M428G/N434A substitutions in its Fc region (blue) still bound PG, while the same antibody with the added Fab substitution T209G (green) or K213V (red) was found in the flow through (Eu numbering).

    https://www.bioz.com/result/igg1 igg3 fc/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg1 igg3 fc - by Bioz Stars, 2020-07
    97/100 stars

    Related Products / Commonly Used Together

    cdrs
    cdna coding sequences
    igg1 hc
    vh1-69
    fabs

    Images

    1) Product Images from "Single-step Protein A and Protein G avidity purification methods to support bispecific antibody discovery and development"

    Article Title: Single-step Protein A and Protein G avidity purification methods to support bispecific antibody discovery and development

    Journal: mAbs

    doi: 10.1080/19420862.2019.1660564

    PA and PG binding assessment of engineered antibodies by linear-gradient chromatography. (a) Overlay of HiTrap® MabSelect™ SuRe™ PA chromatograms (RT). An IgG3-like antibody (IgG 1133) based on the VH3 subclass (blue) still bound the MabSelect™ SuRe™ resin even though elution occurred at a mild pH (~pH 4.2). Adding the Fab substitution G65S (green) or N82aS (red) completely abolished binding (Kabat numbering). (b) Overlay of HiTrap® PG HP chromatograms (RT). An IgG1 antibody carrying the M428G/N434A substitutions in its Fc region (blue) still bound PG, while the same antibody with the added Fab substitution T209G (green) or K213V (red) was found in the flow through (Eu numbering).
    Figure Legend Snippet: PA and PG binding assessment of engineered antibodies by linear-gradient chromatography. (a) Overlay of HiTrap® MabSelect™ SuRe™ PA chromatograms (RT). An IgG3-like antibody (IgG 1133) based on the VH3 subclass (blue) still bound the MabSelect™ SuRe™ resin even though elution occurred at a mild pH (~pH 4.2). Adding the Fab substitution G65S (green) or N82aS (red) completely abolished binding (Kabat numbering). (b) Overlay of HiTrap® PG HP chromatograms (RT). An IgG1 antibody carrying the M428G/N434A substitutions in its Fc region (blue) still bound PG, while the same antibody with the added Fab substitution T209G (green) or K213V (red) was found in the flow through (Eu numbering).

    Techniques Used: Binding Assay, Chromatography, Flow Cytometry

    Single-step avidity purification of a full-length heterodimeric common light chain IgG. (a) Schematic of single-step PG avidity purification. The Fab #1 dG homodimer did not significantly bind PG, the heterodimeric IgG was eluted with glycine buffer pH 3.2, and Fab #2 IgG1 antibody was stripped from the column with glycine buffer pH 2.5. (b) HiTrap® PG HP chromatogram at 4 °C, pH measurements of the main peak fractions are indicated. (c) Total ion chromatogram obtained by RP-HPLC-MS of the main peak (1) fraction with baseline-separation between homo- and heterodimers. Fractional abundances were obtained by integrating the extracted ion chromatograms of homo- and heterodimer species (inset). (G-) denotes a nonfunctional PG binding site.
    Figure Legend Snippet: Single-step avidity purification of a full-length heterodimeric common light chain IgG. (a) Schematic of single-step PG avidity purification. The Fab #1 dG homodimer did not significantly bind PG, the heterodimeric IgG was eluted with glycine buffer pH 3.2, and Fab #2 IgG1 antibody was stripped from the column with glycine buffer pH 2.5. (b) HiTrap® PG HP chromatogram at 4 °C, pH measurements of the main peak fractions are indicated. (c) Total ion chromatogram obtained by RP-HPLC-MS of the main peak (1) fraction with baseline-separation between homo- and heterodimers. Fractional abundances were obtained by integrating the extracted ion chromatograms of homo- and heterodimer species (inset). (G-) denotes a nonfunctional PG binding site.

    Techniques Used: Purification, High Performance Liquid Chromatography, Mass Spectrometry, Binding Assay

    Single-step avidity purification of heterodimeric IgGs. (a) Schematic of single-step PA avidity purification. The IgG dA homodimer did not bind PA, the heterodimeric IgG was eluted with NaAc pH 4.2 (4 °C) or 4.1 (RT), and the scFv-Fc homodimer was stripped from the column with glycine buffer pH 3 (RT or 4 °C). (b) HiTrap® MabSelect SuRe™ chromatogram at 4 °C, pH measurements of the main peak fractions are indicated. (c) Total ion chromatogram obtained by RP-HPLC-MS of the main peak (1) fraction with baseline-separation between homo- and heterodimers. Fractional abundances were obtained by integrating the extracted ion chromatograms of homo- and heterodimer species (inset). (d) Schematic of single-step PG avidity purification. The scFv dG homodimer did not significantly bind PG, the heterodimeric IgG was eluted with glycine buffer pH 3.2 (RT or 4 °C), and the IgG1 antibody was stripped from the column with glycine buffer pH 2.5 (RT or 4 °C). (e) HiTrap® PG HP chromatogram at 4 °C, pH measurements of the main peak fractions are indicated. (f) RP-HPLC-MS analysis of the main peak (1) fraction as in (c). (A-) denotes a nonfunctional PA binding site. (G-) denotes a nonfunctional PG binding site.
    Figure Legend Snippet: Single-step avidity purification of heterodimeric IgGs. (a) Schematic of single-step PA avidity purification. The IgG dA homodimer did not bind PA, the heterodimeric IgG was eluted with NaAc pH 4.2 (4 °C) or 4.1 (RT), and the scFv-Fc homodimer was stripped from the column with glycine buffer pH 3 (RT or 4 °C). (b) HiTrap® MabSelect SuRe™ chromatogram at 4 °C, pH measurements of the main peak fractions are indicated. (c) Total ion chromatogram obtained by RP-HPLC-MS of the main peak (1) fraction with baseline-separation between homo- and heterodimers. Fractional abundances were obtained by integrating the extracted ion chromatograms of homo- and heterodimer species (inset). (d) Schematic of single-step PG avidity purification. The scFv dG homodimer did not significantly bind PG, the heterodimeric IgG was eluted with glycine buffer pH 3.2 (RT or 4 °C), and the IgG1 antibody was stripped from the column with glycine buffer pH 2.5 (RT or 4 °C). (e) HiTrap® PG HP chromatogram at 4 °C, pH measurements of the main peak fractions are indicated. (f) RP-HPLC-MS analysis of the main peak (1) fraction as in (c). (A-) denotes a nonfunctional PA binding site. (G-) denotes a nonfunctional PG binding site.

    Techniques Used: Purification, High Performance Liquid Chromatography, Mass Spectrometry, Binding Assay

    PK profiles of engineered antibodies and heterodimeric IgGs in Sprague-Dawley rats. Blood concentrations over time are shown. Antibodies and heterodimers were administrated at 10 mg/kg in a single intravenous bolus injection. Data points represent the mean concentration ± SD. (a) PK profile comparison of engineered antibodies: trastuzumab dA (IgG dA) and trastuzumab dG (IgG dG) vs. trastuzumab analog (IgG1). (b) PK profile comparison of the different heterodimeric IgGs. The trastuzumab analog profile (IgG1) generated from the same in vivo experiments is shown, heterodimers as indicated. (A-) denotes a nonfunctional PA binding site. (G-) denotes a nonfunctional PG binding site. (*) denotes the presence of the L234A and L235A substitutions in both BEAT Fc chains (Eu numbering).
    Figure Legend Snippet: PK profiles of engineered antibodies and heterodimeric IgGs in Sprague-Dawley rats. Blood concentrations over time are shown. Antibodies and heterodimers were administrated at 10 mg/kg in a single intravenous bolus injection. Data points represent the mean concentration ± SD. (a) PK profile comparison of engineered antibodies: trastuzumab dA (IgG dA) and trastuzumab dG (IgG dG) vs. trastuzumab analog (IgG1). (b) PK profile comparison of the different heterodimeric IgGs. The trastuzumab analog profile (IgG1) generated from the same in vivo experiments is shown, heterodimers as indicated. (A-) denotes a nonfunctional PA binding site. (G-) denotes a nonfunctional PG binding site. (*) denotes the presence of the L234A and L235A substitutions in both BEAT Fc chains (Eu numbering).

    Techniques Used: Injection, Concentration Assay, Generated, In Vivo, Binding Assay

    Related Articles

    Western Blot:

    Article Title: The Deleted in Brachydactyly B Domain of ROR2 Is Required for Receptor Activation by Recruitment of Src
    Article Snippet: .. Primary antibodies used in this study were: mouse anti-IgG-Fc-HRP conjugate and mouse anti-IgG (Pierce); mouse anti-myc; rabbit anti-phospho-Src Y416 (Cell Signalling); the anti-phosphotyrosine cocktail used in western blotting comprised mouse anti-phospho-tyrosine clone 4G10™ (Upstate) and clone pY20 (ICN Biomedicals, Inc.); rabbit anti-Rab5A and rabbit anti-cSrc (Santa Cruz). .. The secondary antibodies were anti-mouse- and anti-rabbit-IgG Horseradish Peroxidase conjugates (Amersham).

    Article Title: Completion of the entire hepatitis C virus life-cycle in genetically humanized mice
    Article Snippet: .. Following secondary antibody staining with HRP-conjugated anti-mouse IgG Fc (JIR, 1:10000), Western blots were visualized using SuperSignal West Pico (Thermo Scientific). .. RT-PCR quantification of HCV entry factors and interferon-stimulated genes (ISGs) To quantify expression of human and murine genes (entry factors and ISGs), the livers of FVB/NJ mice were harvested at the indicated time points.

    Incubation:

    Article Title: Successful selection of an infection‐protective anti‐Staphylococcus aureus monoclonal antibody and its protective activity in murine infection models
    Article Snippet: .. After washing three times with PBST, 100 µL of an anti‐mouse IgG, IgM (Invitrogen, Carlsbad, CA, USA), or anti‐mouse IgG(γ) (Kirkegaard and Perry Laboratories, Gaithersburg, MD, USA) F(ab')2 –HRP‐conjugate was added to each well and the plates incubated at 30 °C for 2 hr. ..

    Binding Assay:

    Article Title: The Molecular Engineering of an Anti-Idiotypic Antibody for Pharmacokinetic Analysis of a Fully Human Anti-Infective
    Article Snippet: .. The binding of the antibodies was detected with anti-mouse-IgG-Fc-HRP (Thermo Scientific, USA) and the reaction developed with TMB substrate. ..

    SDS Page:

    Article Title: Fine mapping and conditional analysis identify a new mutation in the autoimmunity susceptibility gene BLK that leads to reduced half-life of the BLK protein
    Article Snippet: .. A fifth of the extract was resolved on SDS-PAGE and immmunoblotting carried out using standard protocols with anti-V5 antibodies (Invitrogen), b-tubulin (Sigma-Aldrich) and antimouse IgG-HRP (Zymed, San Francisco, California, USA). ..

    Staining:

    Article Title: Completion of the entire hepatitis C virus life-cycle in genetically humanized mice
    Article Snippet: .. Following secondary antibody staining with HRP-conjugated anti-mouse IgG Fc (JIR, 1:10000), Western blots were visualized using SuperSignal West Pico (Thermo Scientific). .. RT-PCR quantification of HCV entry factors and interferon-stimulated genes (ISGs) To quantify expression of human and murine genes (entry factors and ISGs), the livers of FVB/NJ mice were harvested at the indicated time points.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Thermo Fisher hrp conjugated anti mouse igg
    Expression of various envelope proteins on transfected cells and lentiviral vectors. (A) 293T cells transfected with control vector (black line) or envelope protein expression vectors (red line) were stained with anti-Sindbis virus antibody or biotinylated FITC and analyzed by flow cytometry. Flow cytometric profiles and mean fluorescence intensities of staining with anti-Sindbis virus envelope protein antibody and biotinylated FITC are shown. (B) Western blotting analysis of lentiviral vectors pseudotyped with: ① 2.2 1L1L, ② E2 71 AV, ③ E2 71 STAV, ④ E2 71 eMA, ⑤ E2 71 mSAH, or ⑥ 2.2. The same amounts of vectors (110 ng p24) were subjected to SDS-PAGE. After western blotting, the blotted membranes were stained with anti-Sindbis virus antibody, biotinylated <t>HRP,</t> or rabbit <t>IgG-conjugated</t> HRP.
    Hrp Conjugated Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated anti mouse igg/product/Thermo Fisher
    Average 97 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated anti mouse igg - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    89
    Thermo Fisher copy number variation fcgrt hs02236230 cn
    Figure 4. Cetuximab concentrations measured in the patient with 3 copies of <t>FCGRT</t> and mean cetuximab concentration in 93 other patients. Model-predicted concentrations, using the estimated pharmacokinetic parameters, are displayed. Black profile
    Copy Number Variation Fcgrt Hs02236230 Cn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/copy number variation fcgrt hs02236230 cn/product/Thermo Fisher
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    copy number variation fcgrt hs02236230 cn - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    95
    Thermo Fisher snp fcgr2a c 9077561 20
    Kaplan‐Meier curves for overall survival by <t>FCGR2A</t> and FCGR3A polymorphisms under the dominant genetic model assumptions
    Snp Fcgr2a C 9077561 20, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snp fcgr2a c 9077561 20/product/Thermo Fisher
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    snp fcgr2a c 9077561 20 - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    Expression of various envelope proteins on transfected cells and lentiviral vectors. (A) 293T cells transfected with control vector (black line) or envelope protein expression vectors (red line) were stained with anti-Sindbis virus antibody or biotinylated FITC and analyzed by flow cytometry. Flow cytometric profiles and mean fluorescence intensities of staining with anti-Sindbis virus envelope protein antibody and biotinylated FITC are shown. (B) Western blotting analysis of lentiviral vectors pseudotyped with: ① 2.2 1L1L, ② E2 71 AV, ③ E2 71 STAV, ④ E2 71 eMA, ⑤ E2 71 mSAH, or ⑥ 2.2. The same amounts of vectors (110 ng p24) were subjected to SDS-PAGE. After western blotting, the blotted membranes were stained with anti-Sindbis virus antibody, biotinylated HRP, or rabbit IgG-conjugated HRP.

    Journal: Virology

    Article Title: Versatile targeting system for lentiviral vectors involving biotinylated targeting molecules

    doi: 10.1016/j.virol.2018.09.017

    Figure Lengend Snippet: Expression of various envelope proteins on transfected cells and lentiviral vectors. (A) 293T cells transfected with control vector (black line) or envelope protein expression vectors (red line) were stained with anti-Sindbis virus antibody or biotinylated FITC and analyzed by flow cytometry. Flow cytometric profiles and mean fluorescence intensities of staining with anti-Sindbis virus envelope protein antibody and biotinylated FITC are shown. (B) Western blotting analysis of lentiviral vectors pseudotyped with: ① 2.2 1L1L, ② E2 71 AV, ③ E2 71 STAV, ④ E2 71 eMA, ⑤ E2 71 mSAH, or ⑥ 2.2. The same amounts of vectors (110 ng p24) were subjected to SDS-PAGE. After western blotting, the blotted membranes were stained with anti-Sindbis virus antibody, biotinylated HRP, or rabbit IgG-conjugated HRP.

    Article Snippet: Biotin and APC-conjugated anti-hTfR1 antibody, Alexa 488 and HRP-conjugated streptavidin, goat Alexa 488 and HRP-conjugated anti-mouse IgG, Alexa 488-conjugated goat anti-rabbit IgG, and biotin-conjugated EGF were purchased from ThermoFisher Scientific (Canoga Park, CA).

    Techniques: Expressing, Transfection, Plasmid Preparation, Staining, Flow Cytometry, Fluorescence, Western Blot, SDS Page

    Figure 4. Cetuximab concentrations measured in the patient with 3 copies of FCGRT and mean cetuximab concentration in 93 other patients. Model-predicted concentrations, using the estimated pharmacokinetic parameters, are displayed. Black profile

    Journal: mAbs

    Article Title: Influence of FCGRT gene polymorphisms on pharmacokinetics of therapeutic antibodies

    doi: 10.4161/mabs.24815

    Figure Lengend Snippet: Figure 4. Cetuximab concentrations measured in the patient with 3 copies of FCGRT and mean cetuximab concentration in 93 other patients. Model-predicted concentrations, using the estimated pharmacokinetic parameters, are displayed. Black profile

    Article Snippet: The means of the ratios for the 94 patients were 1.02 ± 0.06 and 1.04 ± 0.08 for Hs02236230 and Hs02001837, respectively.

    Techniques: Concentration Assay

    Figure 2. Schematic representation of human FCGRT gene. Chromosome (chr) localization: chr19. Coding and non-coding regions are represented by gray or black boxes respectively. Hs02236230 and Hs02001837 primers used in PCR assays are represented

    Journal: mAbs

    Article Title: Influence of FCGRT gene polymorphisms on pharmacokinetics of therapeutic antibodies

    doi: 10.4161/mabs.24815

    Figure Lengend Snippet: Figure 2. Schematic representation of human FCGRT gene. Chromosome (chr) localization: chr19. Coding and non-coding regions are represented by gray or black boxes respectively. Hs02236230 and Hs02001837 primers used in PCR assays are represented

    Article Snippet: The means of the ratios for the 94 patients were 1.02 ± 0.06 and 1.04 ± 0.08 for Hs02236230 and Hs02001837, respectively.

    Techniques: Polymerase Chain Reaction

    Figure 3. Affect of a supplemental coding sequence of FCGRT on PCR ratios. (A) Results of real-time PCR with Hs02236230 (black bars) and Hs02001837 (gray bars). Ratio values are indicated above respective bars. (B) Southern blot analysis following

    Journal: mAbs

    Article Title: Influence of FCGRT gene polymorphisms on pharmacokinetics of therapeutic antibodies

    doi: 10.4161/mabs.24815

    Figure Lengend Snippet: Figure 3. Affect of a supplemental coding sequence of FCGRT on PCR ratios. (A) Results of real-time PCR with Hs02236230 (black bars) and Hs02001837 (gray bars). Ratio values are indicated above respective bars. (B) Southern blot analysis following

    Article Snippet: The means of the ratios for the 94 patients were 1.02 ± 0.06 and 1.04 ± 0.08 for Hs02236230 and Hs02001837, respectively.

    Techniques: Sequencing, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Southern Blot

    CNV of FCGRT and cetuximab PK

    Journal: mAbs

    Article Title: Influence of FCGRT gene polymorphisms on pharmacokinetics of therapeutic antibodies

    doi: 10.4161/mabs.24815

    Figure Lengend Snippet: CNV of FCGRT and cetuximab PK

    Article Snippet: The means of the ratios for the 94 patients were 1.02 ± 0.06 and 1.04 ± 0.08 for Hs02236230 and Hs02001837, respectively.

    Techniques:

    Kaplan‐Meier curves for overall survival by FCGR2A and FCGR3A polymorphisms under the dominant genetic model assumptions

    Journal: Cancer Medicine

    Article Title: Fc‐gamma receptor polymorphisms, cetuximab therapy, and overall survival in the CCTG CO.20 trial of metastatic colorectal cancer, et al. Fc‐gamma receptor polymorphisms, cetuximab therapy, and overall survival in the CCTG CO.20 trial of metastatic colorectal cancer

    doi: 10.1002/cam4.1819

    Figure Lengend Snippet: Kaplan‐Meier curves for overall survival by FCGR2A and FCGR3A polymorphisms under the dominant genetic model assumptions

    Article Snippet: Assay IDs were C_9077561_20 for rs1801274, and C_25815666_10 for rs396991, with a subset of 30 confirmed also by Sanger sequencing.

    Techniques: