Structured Review

SouthernBiotech igg isotype igg1
Experiment 2. IFA <t>IgG,</t> <t>IgG1,</t> IgG2a, IgG2b, and IgG3 responses against asexual stage P. yoelii parasites determined in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). Bars represent geometric mean IFA titers, and solid circles represent responses in individual mice.
Igg Isotype Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine"

Article Title: CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine

Journal: Infection and Immunity

doi: 10.1128/IAI.72.2.949-957.2004

Experiment 2. IFA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses against asexual stage P. yoelii parasites determined in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). Bars represent geometric mean IFA titers, and solid circles represent responses in individual mice.
Figure Legend Snippet: Experiment 2. IFA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses against asexual stage P. yoelii parasites determined in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). Bars represent geometric mean IFA titers, and solid circles represent responses in individual mice.

Techniques Used: Immunofluorescence, Mouse Assay

Experiment 1. ELISA IgG responses in C57BL/6 mice immunized with yMSP1 19 plus CFA (A), yMSP1 19 plus ODN 1826 plus ISA (B), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (C), ODN 1826 plus ISA (D), yMSP1 19 plus ODN 1826 (E), ODN 1826 (F), and ODN 1982 (G). ELISA titers shown are calculated values determined as interpolated titers at an OD of 0.5. Open bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.
Figure Legend Snippet: Experiment 1. ELISA IgG responses in C57BL/6 mice immunized with yMSP1 19 plus CFA (A), yMSP1 19 plus ODN 1826 plus ISA (B), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (C), ODN 1826 plus ISA (D), yMSP1 19 plus ODN 1826 (E), ODN 1826 (F), and ODN 1982 (G). ELISA titers shown are calculated values determined as interpolated titers at an OD of 0.5. Open bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.

Techniques Used: Enzyme-linked Immunosorbent Assay, Mouse Assay

Experiment 2. ELISA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). ELISA titers shown are calculated geometric mean values determined as interpolated titers at an OD of 0.5. Bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.
Figure Legend Snippet: Experiment 2. ELISA IgG, IgG1, IgG2a, IgG2b, and IgG3 responses in C57BL/6 mice immunized with yMSP1 19 plus ODN 1826 plus ISA (A), yMSP1 19 plus ODN 1982 (control CpG) plus ISA (B), and yMSP1 19 plus ODN 1826 (C). ELISA titers shown are calculated geometric mean values determined as interpolated titers at an OD of 0.5. Bars represent geometric mean ELISA titers, and solid circles represent responses in individual mice.

Techniques Used: Enzyme-linked Immunosorbent Assay, Mouse Assay

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Article Title: A dually active anthrax vaccine that confers protection against both bacilli and toxins
Article Snippet: Standard curves were obtained for each plate by using goat F(ab′)2 anti-mouse Ig as the capturing agent and known concentrations of mouse IgM, IgG, IgG1, IgG2a, IgG2b, and IgG3 as standards.

Mouse Assay:

Article Title: Schistosoma mansoni SmKI-1 or Its C-Terminal Fragment Induces Partial Protection Against S. mansoni Infection in Mice
Article Snippet: The measurement of IgG isotypes revealed that immunization induced the production of antigen-specific IgG1 (Figure B) and IgG2c (Figure C) after the second injection. .. When compared to mice immunized with SmKI-1, KI-domain-immunized animals produced lower levels of both IgG1 and IgG2c. .. Antibody binding to recombinant proteins was investigated using two different methodologies, ELISA (Figure D) and Western blot (Figure E).

Produced:

Article Title: Schistosoma mansoni SmKI-1 or Its C-Terminal Fragment Induces Partial Protection Against S. mansoni Infection in Mice
Article Snippet: The measurement of IgG isotypes revealed that immunization induced the production of antigen-specific IgG1 (Figure B) and IgG2c (Figure C) after the second injection. .. When compared to mice immunized with SmKI-1, KI-domain-immunized animals produced lower levels of both IgG1 and IgG2c. .. Antibody binding to recombinant proteins was investigated using two different methodologies, ELISA (Figure D) and Western blot (Figure E).

Article Title: Distinct B-cell populations contribute to vaccine antigen-specific antibody production in a transgenic mouse model
Article Snippet: IgG2a, IgG2b and IgG2c isotype antibodies were also found at much higher levels in CD43+ (B220− ) cell fractions with low YFP+ cell percentages from both primed and boosted ROSA YFP mice compared with those in CD43− (B220+ ) cell fractions with higher YFP+ cell percentages. .. It is also interesting to note that IgG2b and IgG2c antibodies were produced at detectable levels in the CD43− (B220+ population) cell fraction of boosted splenocytes, although their levels were lower compared with those in CD43+ cell fractions (Fig. c). .. These results indicate that YFP+ ROSA mice can generate vaccine antigen-specific memory B cells capable of differentiating to IgG and isotype antibody-secreting plasma cells after VLP vaccination.

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  • 94
    SouthernBiotech mouse igg isotype control
    A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse <t>IgG</t> (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).
    Mouse Igg Isotype Control, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse igg isotype control/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse igg isotype control - by Bioz Stars, 2021-06
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    95
    SouthernBiotech isotype specific anti igg2a
    Humoral response of Tssc6 gt/gt mice to immunization with T-cell-dependent and T-cell-independent antigens. The T-cell-dependent antigen NP 18 -KLH was administered i.p. (arrowheads) at the following doses for the primary response. (A) One hundred micrograms of NP 18 -KLH precipitated in alum. (B) Ten micrograms of NP 18 -KLH precipitated in alum. (C) One microgram of NP 18 -KLH precipitated in alum. (D) Twenty micrograms of NP 18 -KLH without adjuvant. Boosting occurred where indicated by the second arrow. (E) Fifty micrograms of NP-LPS injected i.p. on day 0 into each mouse to generate a T-cell-independent immune response. Anti-NP IgM and <t>IgG3</t> were measured by ELISA at the indicated time points. Data represent the means and standard errors of the means for six mice (panels A through D) or cohorts of three mice (panel E) of each genotype.
    Isotype Specific Anti Igg2a, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isotype specific anti igg2a/product/SouthernBiotech
    Average 95 stars, based on 1 article reviews
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    93
    SouthernBiotech anti human igg isotype
    Spike-protein specific antibody titers in patient serum samples. Anti-SARS-CoV-2 spike protein total <t>IgG</t> (A), IgA (B), <t>IgG1</t> (C) and IgG3 (D) were measured by ELISA at a 1:50 serum dilution. Data are presented as optical density units (ODU) in relation to the time serum was obtained after admission (n=90) for IgG (Spearman, r=0.35, p=0.0008), IgA (r=0.35, p=0.0008), IgG1 (r=0.31, p=0.01) and IgG3 (r=0.24, p=0.047). (E) Shown is the ratio of anti-SARS-CoV-2 Spike protein-specific IgG1 to IgG3 in 71 patients with detectable IgG (Group 1, n=23; Group 2, n=10; Group 3, n=21, and Group 4, n=17) (*p
    Anti Human Igg Isotype, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human igg isotype/product/SouthernBiotech
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    Price from $9.99 to $1999.99
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    95
    SouthernBiotech igg isotype specific antibodies
    Serum <t>IgM,</t> <t>IgG,</t> and IgA levels over time in the patient.
    Igg Isotype Specific Antibodies, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg isotype specific antibodies/product/SouthernBiotech
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    Image Search Results


    A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).

    Journal: International Journal of Oncology

    Article Title: Molecular basis of antibody binding to mucin glycopeptides in lung cancer

    doi: 10.3892/ijo.2015.3302

    Figure Lengend Snippet: A glycopeptide epitope on lung cancer cell surface is preferably recognized by mAb 16A. Lung adenocarcinoma cell lines, NCI-H1395, HCC4019, H838, H1573, H1703, H2030, and H3255 were studied by flow cytometry staining. Monoclonal antibodies 14A, which binds to MUC1 peptide part only (RPAPGSTAPPAHG); 16A, which binds to MUC1 glycopeptide RPAPGS(GalNAc)TAPPAHG; and B72.3, which binds to sugars only (clustered Tn antigen), were used as primary antibodies. Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).

    Article Snippet: Goat anti-mouse IgG (Allophycocyanin-conjugated), and mouse IgG isotype control were from Southern Biotech (Birmingham, AL, USA).

    Techniques: Flow Cytometry, Cytometry, Staining

    Humoral response of Tssc6 gt/gt mice to immunization with T-cell-dependent and T-cell-independent antigens. The T-cell-dependent antigen NP 18 -KLH was administered i.p. (arrowheads) at the following doses for the primary response. (A) One hundred micrograms of NP 18 -KLH precipitated in alum. (B) Ten micrograms of NP 18 -KLH precipitated in alum. (C) One microgram of NP 18 -KLH precipitated in alum. (D) Twenty micrograms of NP 18 -KLH without adjuvant. Boosting occurred where indicated by the second arrow. (E) Fifty micrograms of NP-LPS injected i.p. on day 0 into each mouse to generate a T-cell-independent immune response. Anti-NP IgM and IgG3 were measured by ELISA at the indicated time points. Data represent the means and standard errors of the means for six mice (panels A through D) or cohorts of three mice (panel E) of each genotype.

    Journal: Molecular and Cellular Biology

    Article Title: The Absence of Tssc6, a Member of the Tetraspanin Superfamily, Does Not Affect Lymphoid Development but Enhances In Vitro T-Cell Proliferative Responses

    doi: 10.1128/MCB.22.14.5006-5018.2002

    Figure Lengend Snippet: Humoral response of Tssc6 gt/gt mice to immunization with T-cell-dependent and T-cell-independent antigens. The T-cell-dependent antigen NP 18 -KLH was administered i.p. (arrowheads) at the following doses for the primary response. (A) One hundred micrograms of NP 18 -KLH precipitated in alum. (B) Ten micrograms of NP 18 -KLH precipitated in alum. (C) One microgram of NP 18 -KLH precipitated in alum. (D) Twenty micrograms of NP 18 -KLH without adjuvant. Boosting occurred where indicated by the second arrow. (E) Fifty micrograms of NP-LPS injected i.p. on day 0 into each mouse to generate a T-cell-independent immune response. Anti-NP IgM and IgG3 were measured by ELISA at the indicated time points. Data represent the means and standard errors of the means for six mice (panels A through D) or cohorts of three mice (panel E) of each genotype.

    Article Snippet: The isotype-specific anti-IgG2a (Southern Biotechnology) and anti-IgA (Silenus) secondary antibodies were biotinylated.

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    Spike-protein specific antibody titers in patient serum samples. Anti-SARS-CoV-2 spike protein total IgG (A), IgA (B), IgG1 (C) and IgG3 (D) were measured by ELISA at a 1:50 serum dilution. Data are presented as optical density units (ODU) in relation to the time serum was obtained after admission (n=90) for IgG (Spearman, r=0.35, p=0.0008), IgA (r=0.35, p=0.0008), IgG1 (r=0.31, p=0.01) and IgG3 (r=0.24, p=0.047). (E) Shown is the ratio of anti-SARS-CoV-2 Spike protein-specific IgG1 to IgG3 in 71 patients with detectable IgG (Group 1, n=23; Group 2, n=10; Group 3, n=21, and Group 4, n=17) (*p

    Journal: Science translational medicine

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients

    doi: 10.1126/scitranslmed.abd5487

    Figure Lengend Snippet: Spike-protein specific antibody titers in patient serum samples. Anti-SARS-CoV-2 spike protein total IgG (A), IgA (B), IgG1 (C) and IgG3 (D) were measured by ELISA at a 1:50 serum dilution. Data are presented as optical density units (ODU) in relation to the time serum was obtained after admission (n=90) for IgG (Spearman, r=0.35, p=0.0008), IgA (r=0.35, p=0.0008), IgG1 (r=0.31, p=0.01) and IgG3 (r=0.24, p=0.047). (E) Shown is the ratio of anti-SARS-CoV-2 Spike protein-specific IgG1 to IgG3 in 71 patients with detectable IgG (Group 1, n=23; Group 2, n=10; Group 3, n=21, and Group 4, n=17) (*p

    Article Snippet: Wells were washed three times with PBS-T and then incubated with horse radish peroxidase (HRP)-conjugated anti-human IgG (1:5000, GenScript #A00166), anti-human IgG isotype specific (Southern Biotech; IgG1: clone HP6001, IgG2: clone 31–7-4, IgG3: clone HP6050, IgG4: clone HP6025) or anti-human IgA Antibody (1:2000 dilution, BioLegend #411002) for 1 hour, RT.

    Techniques: Enzyme-linked Immunosorbent Assay

    Serum IgM, IgG, and IgA levels over time in the patient.

    Journal:

    Article Title: CD20 deficiency in humans results in impaired T cell-independent antibody responses

    doi: 10.1172/JCI40231

    Figure Lengend Snippet: Serum IgM, IgG, and IgA levels over time in the patient.

    Article Snippet: The plates were washed, incubated with alkaline phosphatase–conjugated polyclonal goat anti-mouse IgM and IgG isotype-specific antibodies (Southern Biotechnology Associates) for 1 hour at room temperature, and developed using p -nitrophenyl phosphate substrate (Sigma-Aldrich).

    Techniques: