Structured Review

SouthernBiotech igg isotype analysis
Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total <t>IgG</t> and IgM. Data labeled “a” are statistically significantly different [ P
Igg Isotype Analysis, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igg isotype analysis/product/SouthernBiotech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
igg isotype analysis - by Bioz Stars, 2021-06
93/100 stars

Images

1) Product Images from "Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of Mycobacterium tuberculosis"

Article Title: Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of Mycobacterium tuberculosis

Journal: mBio

doi: 10.1128/mBio.01921-14

Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total IgG and IgM. Data labeled “a” are statistically significantly different [ P
Figure Legend Snippet: Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total IgG and IgM. Data labeled “a” are statistically significantly different [ P

Techniques Used: Enzyme-linked Immunosorbent Assay, Mouse Assay, Injection, Labeling

2) Product Images from "M cell targeting by a Claudin 4 targeting peptide can enhance mucosal IgA responses"

Article Title: M cell targeting by a Claudin 4 targeting peptide can enhance mucosal IgA responses

Journal: BMC Biotechnology

doi: 10.1186/1472-6750-12-7

Immune response to Recombinant fusion protein . Recombinant protein vaccine and a basic four week intranasal immunization protocol, showing enhanced IgA responses by CPE targeting. Systemic serum IgG responses were also induced, without improvement by CPE targeting. A, Immunization protocol. B, ELISA results, showing increased IgA response to HA-CPE protein.
Figure Legend Snippet: Immune response to Recombinant fusion protein . Recombinant protein vaccine and a basic four week intranasal immunization protocol, showing enhanced IgA responses by CPE targeting. Systemic serum IgG responses were also induced, without improvement by CPE targeting. A, Immunization protocol. B, ELISA results, showing increased IgA response to HA-CPE protein.

Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay

Comparison of intranasal and subcutaneous immunization protocols . Analysis of antibody responses to intranasal versus subcutaneous immunization shows similar Th2 dominance in the IgG isotype response. A, Immunization protocols showing the difference between the multiple dose intranasal administration and single dose subcutaneous alum administration. B, Differences in mucosal response to immunization showing the absence of mucosal responses in subcutaneously immunized mice despite similar systemic IgG responses. C, Titration of isotype specific ELISAs for anti-HA response showing the IgG1 dominance over IgG2a immune responses.
Figure Legend Snippet: Comparison of intranasal and subcutaneous immunization protocols . Analysis of antibody responses to intranasal versus subcutaneous immunization shows similar Th2 dominance in the IgG isotype response. A, Immunization protocols showing the difference between the multiple dose intranasal administration and single dose subcutaneous alum administration. B, Differences in mucosal response to immunization showing the absence of mucosal responses in subcutaneously immunized mice despite similar systemic IgG responses. C, Titration of isotype specific ELISAs for anti-HA response showing the IgG1 dominance over IgG2a immune responses.

Techniques Used: Mouse Assay, Titration

Related Articles

Incubation:

Article Title: Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression
Article Snippet: .. BMMSC surface molecules analysis WT BMMSCs or TERT −/ − BMMSCs (0.2 × 106 ) were incubated with 1 μg of PE-conjugated antibodies or isotype-matched control IgGs (Southern Biotech) at 4°C for 30 min. After washing with PBS with 2% FBS and 2% paraformaldehyde fixation, samples were analyzed by FACSCalibur flow cytometry with CellQuest software (BD Bioscience). .. In vitro osteogenic differentiation assay BMMSCs and TERT −/ − BMMSCs were cultured under osteogenic culture conditions in medium containing 2 mM β-glycerophosphate (Sigma-Aldrich), 100 μM l -ascorbic acid 2-phosphate and 10 nM dexamethasone (Sigma-Aldrich).

Flow Cytometry:

Article Title: Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression
Article Snippet: .. BMMSC surface molecules analysis WT BMMSCs or TERT −/ − BMMSCs (0.2 × 106 ) were incubated with 1 μg of PE-conjugated antibodies or isotype-matched control IgGs (Southern Biotech) at 4°C for 30 min. After washing with PBS with 2% FBS and 2% paraformaldehyde fixation, samples were analyzed by FACSCalibur flow cytometry with CellQuest software (BD Bioscience). .. In vitro osteogenic differentiation assay BMMSCs and TERT −/ − BMMSCs were cultured under osteogenic culture conditions in medium containing 2 mM β-glycerophosphate (Sigma-Aldrich), 100 μM l -ascorbic acid 2-phosphate and 10 nM dexamethasone (Sigma-Aldrich).

Cytometry:

Article Title: Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression
Article Snippet: .. BMMSC surface molecules analysis WT BMMSCs or TERT −/ − BMMSCs (0.2 × 106 ) were incubated with 1 μg of PE-conjugated antibodies or isotype-matched control IgGs (Southern Biotech) at 4°C for 30 min. After washing with PBS with 2% FBS and 2% paraformaldehyde fixation, samples were analyzed by FACSCalibur flow cytometry with CellQuest software (BD Bioscience). .. In vitro osteogenic differentiation assay BMMSCs and TERT −/ − BMMSCs were cultured under osteogenic culture conditions in medium containing 2 mM β-glycerophosphate (Sigma-Aldrich), 100 μM l -ascorbic acid 2-phosphate and 10 nM dexamethasone (Sigma-Aldrich).

Software:

Article Title: Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression
Article Snippet: .. BMMSC surface molecules analysis WT BMMSCs or TERT −/ − BMMSCs (0.2 × 106 ) were incubated with 1 μg of PE-conjugated antibodies or isotype-matched control IgGs (Southern Biotech) at 4°C for 30 min. After washing with PBS with 2% FBS and 2% paraformaldehyde fixation, samples were analyzed by FACSCalibur flow cytometry with CellQuest software (BD Bioscience). .. In vitro osteogenic differentiation assay BMMSCs and TERT −/ − BMMSCs were cultured under osteogenic culture conditions in medium containing 2 mM β-glycerophosphate (Sigma-Aldrich), 100 μM l -ascorbic acid 2-phosphate and 10 nM dexamethasone (Sigma-Aldrich).

Negative Control:

Article Title: Characterization of the Porcine CLEC12A and Analysis of Its Expression on Blood Dendritic Cell Subsets
Article Snippet: After blocking free binding sites with normal mouse serum, cells were incubated with FITC-conjugated anti-CD14 (MIL-2) and streptavidin BV421. .. Isotype-matched irrelevant chicken IgY, mouse IgG2b, biotin-labeled mouse IgG1 and FITC-conjugated mouse IgG2b were used as negative control antibodies. .. For sorting of pDCs for cytokine analysis, blood pDCs previously enriched by magnetic depletion as described above were incubated with mAbs to CD172a (BA1C11, IgG1) and CD4 (74-12-4, IgG2b) for 20 min at 4°C.

Expressing:

Article Title: B Cell Development and T-Dependent Antibody Response Are Regulated by p38γ and p38δ
Article Snippet: Cell analysis was performed in a FACScalibur, Beckman Coulter CYTOMIX FC500 MCL and LSR-II cytometer (BD Biosciences). .. Biotinylated goat anti-mouse IgG1, IgG2a, IgG2b, and IgG3 antibodies (Southern Biotech) were used to detect surface expression of IgG isotypes, followed by fluorescently labelled streptavidin (Molecular Probes). .. The profiles obtained were analysed with FlowJo (BD Biosciences) and Kaluza Analysis 2.11 (Beckman Coulter) software; B cells were gated as CD19+ cells when indicated.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    SouthernBiotech igg isotype analysis
    Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total <t>IgG</t> and IgM. Data labeled “a” are statistically significantly different [ P
    Igg Isotype Analysis, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg isotype analysis/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg isotype analysis - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    95
    SouthernBiotech murine anti human igg1
    EBOV-520 Possesses Fc Region Effector Function Activity but Mediates Protection Principally through Virus Neutralization (A) In vitro killing capacity curves for engineered variants of mAb EBOV-520 that determined using SNAP-tagged EBOV GP-expressing 293F cell line as a target and human PBMCs as source of effector cells. Dotted line indicates assay background. (B) Neutralization of EBOV by engineered <t>IgG</t> heavy chain variants of mAb EBOV-520. (C and D) In vivo protective efficacy of EBOV-520 rIgG1 or rIgG1-LALA against EBOV. C57BL/6 mice were challenged with EBOV-MA, treated with indicated mAb in 1 dpi, and monitored for 28 days. Mean ± SD of triplicates are shown, and data are representative of two independent experiments in (A) and (B). Mean ± SEM are shown, and data represent one experiment with five mice per group in (C) and (D). ∗∗ p
    Murine Anti Human Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murine anti human igg1/product/SouthernBiotech
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    murine anti human igg1 - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    Image Search Results


    Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total IgG and IgM. Data labeled “a” are statistically significantly different [ P

    Journal: mBio

    Article Title: Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of Mycobacterium tuberculosis

    doi: 10.1128/mBio.01921-14

    Figure Lengend Snippet: Immunogenicity of mycobacterial vesicles. (A) Inverse titers of M. tuberculosis H37Rv-specific antibodies measured by ELISA in serum from C57BL/6 mice (3 per group) immunized with 2.5 µg of BCG or H37Rv MV using a subcutaneous route of injection or 2 × 10 6 BCG bacilli using the same route after 6 weeks. ELISA was performed on plates coated with 20 µg ml −1 of the indicated mycobacterial subcellular fraction (H37Rv). WCL, whole-cell lysate. (B) Avidity index was determined by titrating ammonium thiocyanate onto plasma total IgG and IgM. Data labeled “a” are statistically significantly different [ P

    Article Snippet: For IgG isotype analysis, IgG1, IgG2c, IgG2b, and IgG3 AP-conjugated secondary antibodies (1:1,000) were used (Southern Biotechnologies).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Injection, Labeling

    Immune response to Recombinant fusion protein . Recombinant protein vaccine and a basic four week intranasal immunization protocol, showing enhanced IgA responses by CPE targeting. Systemic serum IgG responses were also induced, without improvement by CPE targeting. A, Immunization protocol. B, ELISA results, showing increased IgA response to HA-CPE protein.

    Journal: BMC Biotechnology

    Article Title: M cell targeting by a Claudin 4 targeting peptide can enhance mucosal IgA responses

    doi: 10.1186/1472-6750-12-7

    Figure Lengend Snippet: Immune response to Recombinant fusion protein . Recombinant protein vaccine and a basic four week intranasal immunization protocol, showing enhanced IgA responses by CPE targeting. Systemic serum IgG responses were also induced, without improvement by CPE targeting. A, Immunization protocol. B, ELISA results, showing increased IgA response to HA-CPE protein.

    Article Snippet: For IgG isotype analysis, detection was performed using Goat anti-mouse IgG1-AP (Southern Biotech) or Goat anti-mouse IgG2a-AP (Southern Biotech), diluted 1:2000, in 1X TBST.

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

    Comparison of intranasal and subcutaneous immunization protocols . Analysis of antibody responses to intranasal versus subcutaneous immunization shows similar Th2 dominance in the IgG isotype response. A, Immunization protocols showing the difference between the multiple dose intranasal administration and single dose subcutaneous alum administration. B, Differences in mucosal response to immunization showing the absence of mucosal responses in subcutaneously immunized mice despite similar systemic IgG responses. C, Titration of isotype specific ELISAs for anti-HA response showing the IgG1 dominance over IgG2a immune responses.

    Journal: BMC Biotechnology

    Article Title: M cell targeting by a Claudin 4 targeting peptide can enhance mucosal IgA responses

    doi: 10.1186/1472-6750-12-7

    Figure Lengend Snippet: Comparison of intranasal and subcutaneous immunization protocols . Analysis of antibody responses to intranasal versus subcutaneous immunization shows similar Th2 dominance in the IgG isotype response. A, Immunization protocols showing the difference between the multiple dose intranasal administration and single dose subcutaneous alum administration. B, Differences in mucosal response to immunization showing the absence of mucosal responses in subcutaneously immunized mice despite similar systemic IgG responses. C, Titration of isotype specific ELISAs for anti-HA response showing the IgG1 dominance over IgG2a immune responses.

    Article Snippet: For IgG isotype analysis, detection was performed using Goat anti-mouse IgG1-AP (Southern Biotech) or Goat anti-mouse IgG2a-AP (Southern Biotech), diluted 1:2000, in 1X TBST.

    Techniques: Mouse Assay, Titration

    EBOV-520 Possesses Fc Region Effector Function Activity but Mediates Protection Principally through Virus Neutralization (A) In vitro killing capacity curves for engineered variants of mAb EBOV-520 that determined using SNAP-tagged EBOV GP-expressing 293F cell line as a target and human PBMCs as source of effector cells. Dotted line indicates assay background. (B) Neutralization of EBOV by engineered IgG heavy chain variants of mAb EBOV-520. (C and D) In vivo protective efficacy of EBOV-520 rIgG1 or rIgG1-LALA against EBOV. C57BL/6 mice were challenged with EBOV-MA, treated with indicated mAb in 1 dpi, and monitored for 28 days. Mean ± SD of triplicates are shown, and data are representative of two independent experiments in (A) and (B). Mean ± SEM are shown, and data represent one experiment with five mice per group in (C) and (D). ∗∗ p

    Journal: Immunity

    Article Title: Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein

    doi: 10.1016/j.immuni.2018.06.018

    Figure Lengend Snippet: EBOV-520 Possesses Fc Region Effector Function Activity but Mediates Protection Principally through Virus Neutralization (A) In vitro killing capacity curves for engineered variants of mAb EBOV-520 that determined using SNAP-tagged EBOV GP-expressing 293F cell line as a target and human PBMCs as source of effector cells. Dotted line indicates assay background. (B) Neutralization of EBOV by engineered IgG heavy chain variants of mAb EBOV-520. (C and D) In vivo protective efficacy of EBOV-520 rIgG1 or rIgG1-LALA against EBOV. C57BL/6 mice were challenged with EBOV-MA, treated with indicated mAb in 1 dpi, and monitored for 28 days. Mean ± SD of triplicates are shown, and data are representative of two independent experiments in (A) and (B). Mean ± SEM are shown, and data represent one experiment with five mice per group in (C) and (D). ∗∗ p

    Article Snippet: mAb isotype and gene sequence analysis The isotype and subclass of secreted antibodies were determined using murine anti-human IgG1, IgG2, IgG3 or IgG4 mouse antibodies conjugated with alkaline phosphatase (Southern Biotech).

    Techniques: Activity Assay, Neutralization, In Vitro, Expressing, In Vivo, Mouse Assay