igg depletion column  (Millipore)

 
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    Name:
    ProteoPrep Immunoaffinity Albumin IgG Depletion Kit
    Description:
    The ProteoPrep Immunoaffinity medium in the prepacked spin columns is a mixture of two beaded media containing recombinantly expressed small single chain antibody ligands resulting in low non specific binding and high capacity This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum
    Catalog Number:
    protia
    Price:
    None
    Applications:
    The ProteoPrep(R) Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50muL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC).
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    Structured Review

    Millipore igg depletion column
    The ProteoPrep Immunoaffinity medium in the prepacked spin columns is a mixture of two beaded media containing recombinantly expressed small single chain antibody ligands resulting in low non specific binding and high capacity This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum
    https://www.bioz.com/result/igg depletion column/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg depletion column - by Bioz Stars, 2021-02
    92/100 stars

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    Related Articles

    Two-Dimensional Gel Electrophoresis:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Depletion of albumin and IgG by the PROTIA-Sigma kit improved the quality of 2DE gels in two ways: firstly, depletion of albumin and IgG resulted in improved separation within these regions of the gel and the emergence of several new spots (Figure , circles). ..

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Based on 2DE and MS results, the PROTIA-Sigma Kit dramatically improved 2DE gel quality by increasing low abundance protein intensity, and thus serves as a useful tool in novel biomarker discovery. ..

    Biomarker Assay:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Based on 2DE and MS results, the PROTIA-Sigma Kit dramatically improved 2DE gel quality by increasing low abundance protein intensity, and thus serves as a useful tool in novel biomarker discovery. ..

    Produced:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Therefore, the depleted sera by the PROTBA-Sigma and PROTIA-Sigma kits, as well as the concentrated sera produced by the 5185-Agilent kit were all within the appropriate protein concentration range. .. Both the serum load and the protein yield were highest for the PROTIA-Sigma kit, indicating that it was most suitable for large scale protein preparation.

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Among the three kits, the PROTIA-Sigma kit had the highest load volume1 and produced the most depleted serum (Table ). .. The PROTBA-Sigma Kit had the highest initial yield percentage and concentration of the three kits, likely because albumin and IgG were not completely removed by this kit.

    Mass Spectrometry:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Based on 2DE and MS results, the PROTIA-Sigma Kit dramatically improved 2DE gel quality by increasing low abundance protein intensity, and thus serves as a useful tool in novel biomarker discovery. ..

    Protein Concentration:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Therefore, the depleted sera by the PROTBA-Sigma and PROTIA-Sigma kits, as well as the concentrated sera produced by the 5185-Agilent kit were all within the appropriate protein concentration range. .. Both the serum load and the protein yield were highest for the PROTIA-Sigma kit, indicating that it was most suitable for large scale protein preparation.

    Electrofocusing:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Secondly, the depleted serum from the PROTIA-Sigma kit could be used for isoelectric focusing (IEF) directly since the wash solution contained fewer ions. .. Depletion improved 2DE gel quality by two aspects.

    Serum Depletion:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Conversely, both immunoaffinity based kits, the 5185-Agilent kit and the PROTIA-Sigma kit, had higher efficiency in umbilical cord serum depletion. ..

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  • 93
    Millipore igg subclass antibodies
    Effects of <t>IgG1</t> autoantibodies on renal function and renal pathology in MRL- lpr/lpr mice. Representative fluorescence microscopy images (A) and statistical analysis (B) of C1q, C3, IgG1, and <t>IgG3</t> deposition in the kidney of non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice ( n = 6) at 19 weeks of age (scale bars, 50 μm). Serum creatinine, BUN, and urinary proteins (C) , and light-microscopy examination of the histopathology of glomerulonephritis using H E, PAS, and Masson's staining (D) in non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice ( n = 6) at 19 weeks of age (scale bars, 50 μm). * P
    Igg Subclass Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg subclass antibodies/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    igg subclass antibodies - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effects of IgG1 autoantibodies on renal function and renal pathology in MRL- lpr/lpr mice. Representative fluorescence microscopy images (A) and statistical analysis (B) of C1q, C3, IgG1, and IgG3 deposition in the kidney of non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice ( n = 6) at 19 weeks of age (scale bars, 50 μm). Serum creatinine, BUN, and urinary proteins (C) , and light-microscopy examination of the histopathology of glomerulonephritis using H E, PAS, and Masson's staining (D) in non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice ( n = 6) at 19 weeks of age (scale bars, 50 μm). * P

    Journal: Frontiers in Immunology

    Article Title: IgG4 Autoantibodies Attenuate Systemic Lupus Erythematosus Progression by Suppressing Complement Consumption and Inflammatory Cytokine Production

    doi: 10.3389/fimmu.2020.01047

    Figure Lengend Snippet: Effects of IgG1 autoantibodies on renal function and renal pathology in MRL- lpr/lpr mice. Representative fluorescence microscopy images (A) and statistical analysis (B) of C1q, C3, IgG1, and IgG3 deposition in the kidney of non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice ( n = 6) at 19 weeks of age (scale bars, 50 μm). Serum creatinine, BUN, and urinary proteins (C) , and light-microscopy examination of the histopathology of glomerulonephritis using H E, PAS, and Masson's staining (D) in non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice ( n = 6) at 19 weeks of age (scale bars, 50 μm). * P

    Article Snippet: They also discussed that murine IgG1 and human IgG4 can potentially be used to block complement activation by sialylated IgG-subclass antibodies ( ).

    Techniques: Mouse Assay, Fluorescence, Microscopy, Light Microscopy, Histopathology, Staining

    Analysis of purified human IgG4 and the effect of IgG4 on complement consumption by autoantibody-autoantigen ICs in vitro . Human IgG4 from the sera of healthy control subjects ( n = 12) and newly diagnosed SLE patients ( n = 12) was purified by immunoaffinity column chromatography and the antibody mixtures were analyzed by SDS-PAGE (A) , IF staining with HEp-2 cells (scale bars, 50 μm) (B) , and the ANA Euroline Profile 3 Kit (C) . (D) Schematic representation of the in vitro complement-consumption assay. Three independent experiments were performed. Consumption of complement proteins including C3 (E) , C4 (F) , complement factors B (G) and H (H) , as well as C5b-9 deposits (I) in cultured HEp-2 cells were detected after co-culturing HEp-2 cells with SLE serum, mixed with or without different concentrations of purified IgG4 (the IgG4: IgG ratio was 50 or 100%). As blank control, the first column of white circles from the left represents only sera from SLE patients ( n = 12), without IgG4 and HEp-2 cells (E–I) ; As positive control of the existence of autoantigens, the second column of white circles represents sera from SLE patients ( n = 12), plus HEp-2 cells, but without IgG4 (E–I) . * P

    Journal: Frontiers in Immunology

    Article Title: IgG4 Autoantibodies Attenuate Systemic Lupus Erythematosus Progression by Suppressing Complement Consumption and Inflammatory Cytokine Production

    doi: 10.3389/fimmu.2020.01047

    Figure Lengend Snippet: Analysis of purified human IgG4 and the effect of IgG4 on complement consumption by autoantibody-autoantigen ICs in vitro . Human IgG4 from the sera of healthy control subjects ( n = 12) and newly diagnosed SLE patients ( n = 12) was purified by immunoaffinity column chromatography and the antibody mixtures were analyzed by SDS-PAGE (A) , IF staining with HEp-2 cells (scale bars, 50 μm) (B) , and the ANA Euroline Profile 3 Kit (C) . (D) Schematic representation of the in vitro complement-consumption assay. Three independent experiments were performed. Consumption of complement proteins including C3 (E) , C4 (F) , complement factors B (G) and H (H) , as well as C5b-9 deposits (I) in cultured HEp-2 cells were detected after co-culturing HEp-2 cells with SLE serum, mixed with or without different concentrations of purified IgG4 (the IgG4: IgG ratio was 50 or 100%). As blank control, the first column of white circles from the left represents only sera from SLE patients ( n = 12), without IgG4 and HEp-2 cells (E–I) ; As positive control of the existence of autoantigens, the second column of white circles represents sera from SLE patients ( n = 12), plus HEp-2 cells, but without IgG4 (E–I) . * P

    Article Snippet: They also discussed that murine IgG1 and human IgG4 can potentially be used to block complement activation by sialylated IgG-subclass antibodies ( ).

    Techniques: Purification, In Vitro, Column Chromatography, SDS Page, Staining, Cell Culture, Positive Control

    Analysis of purified IgG4 autoantigens and their effect on inflammatory cytokine production by SLE PBMCs in vitro . Purified nuclear autoantigens from HEp-2 cells were analyzed by SDS-PAGE (A) , and serum anti-nuclear IgG autoantibodies from healthy control subjects and SLE patients were detected by ELISA (B) . (C) Schematic representation of the in vitro inflammatory cytokine assays. Three independent experiments were performed. The production of inflammatory cytokines, including IFN-γ (D) , IL-6 (E) , and IL-17 (F) by autoantigen-stimulated SLE PBMCs were detected, mixed with or without different concentrations of purified IgG4 (the IgG4: IgG ratio was 50 or 100%). As blank control, the first column of white circles from the left represents only SLE PBMCs ( n = 12, grown in DMEM/F12 supplemented with 25% serum of SLE patients), without IgG4 and nuclear antigens (D–F) ; As positive control of the existence of nuclear antigens, the second column of white circles represents SLE PBMCs ( n = 12, grown in DMEM/F12 supplemented with 25% serum of SLE patients), plus nuclear antigens, but without IgG4 (D–F) . * P

    Journal: Frontiers in Immunology

    Article Title: IgG4 Autoantibodies Attenuate Systemic Lupus Erythematosus Progression by Suppressing Complement Consumption and Inflammatory Cytokine Production

    doi: 10.3389/fimmu.2020.01047

    Figure Lengend Snippet: Analysis of purified IgG4 autoantigens and their effect on inflammatory cytokine production by SLE PBMCs in vitro . Purified nuclear autoantigens from HEp-2 cells were analyzed by SDS-PAGE (A) , and serum anti-nuclear IgG autoantibodies from healthy control subjects and SLE patients were detected by ELISA (B) . (C) Schematic representation of the in vitro inflammatory cytokine assays. Three independent experiments were performed. The production of inflammatory cytokines, including IFN-γ (D) , IL-6 (E) , and IL-17 (F) by autoantigen-stimulated SLE PBMCs were detected, mixed with or without different concentrations of purified IgG4 (the IgG4: IgG ratio was 50 or 100%). As blank control, the first column of white circles from the left represents only SLE PBMCs ( n = 12, grown in DMEM/F12 supplemented with 25% serum of SLE patients), without IgG4 and nuclear antigens (D–F) ; As positive control of the existence of nuclear antigens, the second column of white circles represents SLE PBMCs ( n = 12, grown in DMEM/F12 supplemented with 25% serum of SLE patients), plus nuclear antigens, but without IgG4 (D–F) . * P

    Article Snippet: They also discussed that murine IgG1 and human IgG4 can potentially be used to block complement activation by sialylated IgG-subclass antibodies ( ).

    Techniques: Purification, In Vitro, SDS Page, Enzyme-linked Immunosorbent Assay, Positive Control

    Effect of IgG1 autoantibodies on complement consumption and inflammatory cytokine production in MRL- lpr/lpr mice. (A) Experimental design and timeline. Serum mouse IgG1 from control mice ( n = 20) and aged MRL- lpr/lpr mice ( n = 20) was purified by immunoaffinity-column chromatography and the antibody mixtures were analyzed by SDS-PAGE (B) and IF staining with HEp-2 cells (scale bars, 50 μm) (C) . (D,E) Serum levels of autoantibodies in non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice at 7, 11, 15, and 19 weeks of age ( n = 6). (F) Representative images of the spleens of non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice at 19 weeks of age (from left to right) and statistical analysis of the spleen weight as a percentage of the body weight ( n = 6). (G) Serum levels of complement proteins including C3, complement factor B, and complement factor H, and (H) inflammatory cytokines including IFN-γ, IL-6, and IL-17 in non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice at 19 weeks of age ( n = 6). * P

    Journal: Frontiers in Immunology

    Article Title: IgG4 Autoantibodies Attenuate Systemic Lupus Erythematosus Progression by Suppressing Complement Consumption and Inflammatory Cytokine Production

    doi: 10.3389/fimmu.2020.01047

    Figure Lengend Snippet: Effect of IgG1 autoantibodies on complement consumption and inflammatory cytokine production in MRL- lpr/lpr mice. (A) Experimental design and timeline. Serum mouse IgG1 from control mice ( n = 20) and aged MRL- lpr/lpr mice ( n = 20) was purified by immunoaffinity-column chromatography and the antibody mixtures were analyzed by SDS-PAGE (B) and IF staining with HEp-2 cells (scale bars, 50 μm) (C) . (D,E) Serum levels of autoantibodies in non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice at 7, 11, 15, and 19 weeks of age ( n = 6). (F) Representative images of the spleens of non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice at 19 weeks of age (from left to right) and statistical analysis of the spleen weight as a percentage of the body weight ( n = 6). (G) Serum levels of complement proteins including C3, complement factor B, and complement factor H, and (H) inflammatory cytokines including IFN-γ, IL-6, and IL-17 in non-treated and control or lupus IgG1-treated MRL- lpr/lpr mice at 19 weeks of age ( n = 6). * P

    Article Snippet: They also discussed that murine IgG1 and human IgG4 can potentially be used to block complement activation by sialylated IgG-subclass antibodies ( ).

    Techniques: Mouse Assay, Purification, Column Chromatography, SDS Page, Staining

    Schematic representation of IgG4 autoantibody-mediated attenuation of disease progression in SLE by suppressing complement consumption and inflammatory cytokine production. During SLE progression, the inflammatory response induced by each autoreactive IgG subtype depends on its ability to activate effector function. IgG4 autoantibodies may bind autoantigens completely with other subclasses of autoantibodies such as IgG1, IgG2, and IgG3, to form autoantibody-autoantigen ICs. The hinge region of IgG4 has a unique structure that determines its limited ability to activate effector function. Thus, compared with other subclasses of autoantibodies, IgG4 autoantibodies may attenuate disease progression in SLE by suppressing complement consumption and inflammatory cytokine production.

    Journal: Frontiers in Immunology

    Article Title: IgG4 Autoantibodies Attenuate Systemic Lupus Erythematosus Progression by Suppressing Complement Consumption and Inflammatory Cytokine Production

    doi: 10.3389/fimmu.2020.01047

    Figure Lengend Snippet: Schematic representation of IgG4 autoantibody-mediated attenuation of disease progression in SLE by suppressing complement consumption and inflammatory cytokine production. During SLE progression, the inflammatory response induced by each autoreactive IgG subtype depends on its ability to activate effector function. IgG4 autoantibodies may bind autoantigens completely with other subclasses of autoantibodies such as IgG1, IgG2, and IgG3, to form autoantibody-autoantigen ICs. The hinge region of IgG4 has a unique structure that determines its limited ability to activate effector function. Thus, compared with other subclasses of autoantibodies, IgG4 autoantibodies may attenuate disease progression in SLE by suppressing complement consumption and inflammatory cytokine production.

    Article Snippet: They also discussed that murine IgG1 and human IgG4 can potentially be used to block complement activation by sialylated IgG-subclass antibodies ( ).

    Techniques:

    Correlation between serum levels of IgG4 autoantibody and disease activity in patients with SLE. (A) Serum levels of ANA-IgG ( n = 106) and ANA-IgG4 in newly diagnosed patients in group I ( n = 37) and group II ( n = 32) were detected by IF staining of HEp-2 cells, and the fluorescence intensities were graded (scale bars, 50 μm). The SLEDAI score (B) , serum levels of inflammatory cytokines including IFN-γ, IL-6, and IL-17 (C) , serum levels of complement proteins including C3, C4, complement factor B, and complement factor H (D) , and serum creatinine, BUN, urine protein: creatinine, and 24-h proteinuria levels (E) in newly diagnosed patients with SLE of group I ( n = 37) and group II ( n = 32) were analyzed. * P

    Journal: Frontiers in Immunology

    Article Title: IgG4 Autoantibodies Attenuate Systemic Lupus Erythematosus Progression by Suppressing Complement Consumption and Inflammatory Cytokine Production

    doi: 10.3389/fimmu.2020.01047

    Figure Lengend Snippet: Correlation between serum levels of IgG4 autoantibody and disease activity in patients with SLE. (A) Serum levels of ANA-IgG ( n = 106) and ANA-IgG4 in newly diagnosed patients in group I ( n = 37) and group II ( n = 32) were detected by IF staining of HEp-2 cells, and the fluorescence intensities were graded (scale bars, 50 μm). The SLEDAI score (B) , serum levels of inflammatory cytokines including IFN-γ, IL-6, and IL-17 (C) , serum levels of complement proteins including C3, C4, complement factor B, and complement factor H (D) , and serum creatinine, BUN, urine protein: creatinine, and 24-h proteinuria levels (E) in newly diagnosed patients with SLE of group I ( n = 37) and group II ( n = 32) were analyzed. * P

    Article Snippet: They also discussed that murine IgG1 and human IgG4 can potentially be used to block complement activation by sialylated IgG-subclass antibodies ( ).

    Techniques: Activity Assay, Staining, Fluorescence