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Greiner Bio iga levels
BALF antibody titers to M2e peptides in mice of experimental and control groups on day 14 post third immunization with Flg-HA2-4M2e and Flg-4M2e-HA2: A – <t>IgG;</t> B – <t>IgA</t>
Iga Levels, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 17 article reviews
Price from $9.99 to $1999.99
iga levels - by Bioz Stars, 2020-07
91/100 stars

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1) Product Images from "Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins"

Article Title: Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins

Journal: Acta Naturae

doi:

BALF antibody titers to M2e peptides in mice of experimental and control groups on day 14 post third immunization with Flg-HA2-4M2e and Flg-4M2e-HA2: A – IgG; B – IgA
Figure Legend Snippet: BALF antibody titers to M2e peptides in mice of experimental and control groups on day 14 post third immunization with Flg-HA2-4M2e and Flg-4M2e-HA2: A – IgG; B – IgA

Techniques Used: Mouse Assay

2) Product Images from "BAFF augments IgA2 and IL‐10 production by TLR7/8 stimulated total peripheral blood B cells"

Article Title: BAFF augments IgA2 and IL‐10 production by TLR7/8 stimulated total peripheral blood B cells

Journal: European Journal of Immunology

doi: 10.1002/eji.201646861

BAFF and Retinoic acid differentially affect IgA1 and IgA2 production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific ELISA. Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p
Figure Legend Snippet: BAFF and Retinoic acid differentially affect IgA1 and IgA2 production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific ELISA. Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p

Techniques Used: Enzyme-linked Immunosorbent Assay

3) Product Images from "Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins"

Article Title: Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins

Journal: Acta Naturae

doi:

BALF antibody titers to M2e peptides in mice of experimental and control groups on day 14 post third immunization with Flg-HA2-4M2e and Flg-4M2e-HA2: A – IgG; B – IgA
Figure Legend Snippet: BALF antibody titers to M2e peptides in mice of experimental and control groups on day 14 post third immunization with Flg-HA2-4M2e and Flg-4M2e-HA2: A – IgG; B – IgA

Techniques Used: Mouse Assay

4) Product Images from "Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases"

Article Title: Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases

Journal: Orphanet Journal of Rare Diseases

doi: 10.1186/1750-1172-6-31

Immunoreactivity of IgA autoantibodies with native and recombinant BP180 ectodomain . Precipitated, recombinant (lanes 1-6) and native, keratinocyte-derived (lanes 7-12) BP180 ectodomain immunoblotted with IgA pemphigoid (LAD) patients' sera (lanes 1-3, 7-9) and control sera (NHS) (lanes 4, 5, 10 and 11). As substrate control (lanes 13-18), precipitated culture medium from cells transfected with empty vector was immunoblotted using the same LAD (lanes 13-15) and NHS (lane 16, 17) sera. Presence or absence of the 120 kDa ectodomain was visualized using a specific monoclonal Ab (lanes 6, 12 and 18).
Figure Legend Snippet: Immunoreactivity of IgA autoantibodies with native and recombinant BP180 ectodomain . Precipitated, recombinant (lanes 1-6) and native, keratinocyte-derived (lanes 7-12) BP180 ectodomain immunoblotted with IgA pemphigoid (LAD) patients' sera (lanes 1-3, 7-9) and control sera (NHS) (lanes 4, 5, 10 and 11). As substrate control (lanes 13-18), precipitated culture medium from cells transfected with empty vector was immunoblotted using the same LAD (lanes 13-15) and NHS (lane 16, 17) sera. Presence or absence of the 120 kDa ectodomain was visualized using a specific monoclonal Ab (lanes 6, 12 and 18).

Techniques Used: Recombinant, Derivative Assay, Transfection, Plasmid Preparation

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Enhanced Immunogenicity of HIV-1 Envelope gp140 Proteins Fused to APRIL
Article Snippet: .. Total IgG/IgA/IgM ELISA Animal sera, treated as described above for the anti-gp120 ELISA, were diluted and coated o/n onto Microlon 96-well plates (half-area, Microlon 600, Greiner Bio-One) in 0.1 M NaHCO3 (50 µl/well). .. The plates were washed twice with TBS, and incubated with 2% skim milk powder in TBS for 30 min to block the residual protein-binding sites.

Article Title: Cross-Protective Immune Responses Induced by Sequential Influenza Virus Infection and by Sequential Vaccination With Inactivated Influenza Vaccines
Article Snippet: .. Elisa For the detection of IgG, IgG1, IgG2a, or IgA antibody against A(H1N1)pdm09 virus in serum and nasal wash, ELISA plates (Greiner, Alphen a/d Rijn, Netherlands) were coated with 0.3 μg/well of X-181 WIV, conserved M2e peptide (SLLTEVETPIRNEWGSRSNDSSD) or NP protein overnight at 37°C and ELISA assays were performed as described before ( ). .. For NA-specific ELISA, recombinant NA protein of A(H1N1)pdm09 was expressed and purified as described previously ( ).

Article Title: Protection against Multiple Influenza A Virus Strains Induced by Candidate Recombinant Vaccine Based on Heterologous M2e Peptides Linked to Flagellin
Article Snippet: .. Antibody detection in the sera and BAL M2e-specific IgG and IgA levels were determined by ELISA in 96-well microtitre plates (“Greiner”, Germany) coated overnight at 4°C with the M2e peptides (100μl/well) in PBS (5 μg/ml). ..

Article Title: Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins
Article Snippet: .. ELISA Antigen-specific IgG and IgA levels in immunized mice were evaluated with ELISA in high adhesion 96-well plates (Greiner, Germany). .. The plates were coated with M2e-peptides (5 μg/ml) or purified virus A/Aichi/2/68 (H3N2) (2 μg/ml) in PBS (pH 7.2) overnight at 4°C.

Article Title: Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins
Article Snippet: .. Antigen-specific IgG and IgA levels in immunized mice were evaluated with ELISA in high adhesion 96-well plates (Greiner, Germany). .. The plates were coated with M2e-peptides (5 μg/ml) or purified virus A/Aichi/2/68 (H3N2) (2 μg/ml) in PBS (pH 7.2) overnight at 4°C.

Article Title: BAFF augments IgA2 and IL‐10 production by TLR7/8 stimulated total peripheral blood B cells
Article Snippet: .. IgA1 and IgA2 ELISA ELISA plates (# 650‐061, Greiner Bio One) were coated overnight with 1.0 μg/mL Polyclonal goat anti human IgA (Southern Biotech, 2050‐01). ..

Recombinant:

Article Title: Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases
Article Snippet: .. For detection of IgA autoantibodies, 96-well microtiter plates (Greiner Bio-One, Germany) were coated with 1.4 μg/well of recombinant BP180 ectodomain in 0.1 M bicarbonate buffer (pH 9.6), overnight at 4 °C. .. After washing with 0.05%Tween20-PBS (w/v) and subsequent 1 h blocking with 2% BSA-PBS (w/v) the plates were incubated for 1 h with 1:50 diluted sera in 1% BSA-0.05% Tween20-PBS (w/v).

Mouse Assay:

Article Title: Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins
Article Snippet: .. ELISA Antigen-specific IgG and IgA levels in immunized mice were evaluated with ELISA in high adhesion 96-well plates (Greiner, Germany). .. The plates were coated with M2e-peptides (5 μg/ml) or purified virus A/Aichi/2/68 (H3N2) (2 μg/ml) in PBS (pH 7.2) overnight at 4°C.

Article Title: Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins
Article Snippet: .. Antigen-specific IgG and IgA levels in immunized mice were evaluated with ELISA in high adhesion 96-well plates (Greiner, Germany). .. The plates were coated with M2e-peptides (5 μg/ml) or purified virus A/Aichi/2/68 (H3N2) (2 μg/ml) in PBS (pH 7.2) overnight at 4°C.

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    Greiner Bio iga2 elisa elisa plates
    BAFF and Retinoic acid differentially affect IgA1 and <t>IgA2</t> production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific <t>ELISA.</t> Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p
    Iga2 Elisa Elisa Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga2 elisa elisa plates/product/Greiner Bio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iga2 elisa elisa plates - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    94
    Greiner Bio elisa plates
    Effect of deglycosylation of <t>CEA</t> on recognition by MS11 Opa variants. ( A ) Immunoblot showing native CEA (CEA) and CEA treated with TFMSA (dg-CEA). Each lane contains 50 ng of material, which was detected with anti-CEA rabbit serum. M r markers (× 10 −3 ) are indicated on the left. ( B ) Binding of MS11 Opa variants to native and deglycosylated CEA. Radiolabeled bacteria were added to the wells of <t>ELISA</t> plates containing native (CEA) or deglycosylated (dg-CEA) human tumor CEA for 1 hr at 37°C. Nonadherent bacteria were removed by washing and the number of bound bacteria was determined by liquid scintillation counting of detached bacteria. GC, gonococci. Data are means ± SE of three independent experiments.
    Elisa Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa plates/product/Greiner Bio
    Average 94 stars, based on 184 article reviews
    Price from $9.99 to $1999.99
    elisa plates - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    BAFF and Retinoic acid differentially affect IgA1 and IgA2 production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific ELISA. Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p

    Journal: European Journal of Immunology

    Article Title: BAFF augments IgA2 and IL‐10 production by TLR7/8 stimulated total peripheral blood B cells

    doi: 10.1002/eji.201646861

    Figure Lengend Snippet: BAFF and Retinoic acid differentially affect IgA1 and IgA2 production in mature stimulated B cells. IgA1 (A,C) and IgA2 (B,D) production by total peripheral blood (A,B) or naïve (C,D) B cells left unstimulated or stimulated for 6 days by CpG‐ODN or R848 in combination with T cell independent B cell class switch inducing factors as measured by IgA1 and IgA2 specific ELISA. Data shown as mean + SEM, A and B: n = 8–14 donors per group, combined graph of five separate experiments * p

    Article Snippet: IgA1 and IgA2 ELISA ELISA plates (# 650‐061, Greiner Bio One) were coated overnight with 1.0 μg/mL Polyclonal goat anti human IgA (Southern Biotech, 2050‐01).

    Techniques: Enzyme-linked Immunosorbent Assay

    Effect of deglycosylation of CEA on recognition by MS11 Opa variants. ( A ) Immunoblot showing native CEA (CEA) and CEA treated with TFMSA (dg-CEA). Each lane contains 50 ng of material, which was detected with anti-CEA rabbit serum. M r markers (× 10 −3 ) are indicated on the left. ( B ) Binding of MS11 Opa variants to native and deglycosylated CEA. Radiolabeled bacteria were added to the wells of ELISA plates containing native (CEA) or deglycosylated (dg-CEA) human tumor CEA for 1 hr at 37°C. Nonadherent bacteria were removed by washing and the number of bound bacteria was determined by liquid scintillation counting of detached bacteria. GC, gonococci. Data are means ± SE of three independent experiments.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CD66 receptor specificity exhibited by neisserial Opa variants is controlled by protein determinants in CD66 N-domains

    doi:

    Figure Lengend Snippet: Effect of deglycosylation of CEA on recognition by MS11 Opa variants. ( A ) Immunoblot showing native CEA (CEA) and CEA treated with TFMSA (dg-CEA). Each lane contains 50 ng of material, which was detected with anti-CEA rabbit serum. M r markers (× 10 −3 ) are indicated on the left. ( B ) Binding of MS11 Opa variants to native and deglycosylated CEA. Radiolabeled bacteria were added to the wells of ELISA plates containing native (CEA) or deglycosylated (dg-CEA) human tumor CEA for 1 hr at 37°C. Nonadherent bacteria were removed by washing and the number of bound bacteria was determined by liquid scintillation counting of detached bacteria. GC, gonococci. Data are means ± SE of three independent experiments.

    Article Snippet: CEA was dried onto the wells of ELISA plates (Greiner, Frickenhausen, Germany) at 1 μg per well.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked immunosorbent spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay (ELISA) with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.

    Journal: The Journal of Infectious Diseases

    Article Title: Distinct Cross-reactive B-Cell Responses to Live Attenuated and Inactivated Influenza Vaccines

    doi: 10.1093/infdis/jiu190

    Figure Lengend Snippet: Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked immunosorbent spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay (ELISA) with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.

    Article Snippet: Ninety-six–well ELISA plates (Greiner) were coated with IIV (2.7 µg/mL), recombinant HAs (Immune Technology, at 5 µg/mL), or recombinant NP (5 µg/mL).

    Techniques: ELISpot Assay, Recombinant, Binding Assay, Activity Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay

    Cross-reactive hemagglutinin (HA) binding activity of plasmablast-derived polyclonal antibodies (PPAbs) after immunization with 2010 inactivated influenza vaccine (IIV) and live attenuated influenza vaccine (LAIV). A pool of PPAbs was assembled by combining equal amounts of immunoglobulin G (IgG) from each PPAb sample in the IIV or LAIV groups. Each PPAb pool was serially diluted, starting at a concentration of 9 ng/mL for LAIV and 4.1 ng/mL for IIV of IgG, and analyzed with an enzyme-linked immunosorbent assay (ELISA) for binding to recombinant HAs from indicated influenza virus strains. Relative binding activity to each HA (numbers in each panel) was defined as the ratio of its area under curve (AUC) to that of the relevant 2010 vaccine strain (circle in each panel, assigned a value of 1).

    Journal: The Journal of Infectious Diseases

    Article Title: Distinct Cross-reactive B-Cell Responses to Live Attenuated and Inactivated Influenza Vaccines

    doi: 10.1093/infdis/jiu190

    Figure Lengend Snippet: Cross-reactive hemagglutinin (HA) binding activity of plasmablast-derived polyclonal antibodies (PPAbs) after immunization with 2010 inactivated influenza vaccine (IIV) and live attenuated influenza vaccine (LAIV). A pool of PPAbs was assembled by combining equal amounts of immunoglobulin G (IgG) from each PPAb sample in the IIV or LAIV groups. Each PPAb pool was serially diluted, starting at a concentration of 9 ng/mL for LAIV and 4.1 ng/mL for IIV of IgG, and analyzed with an enzyme-linked immunosorbent assay (ELISA) for binding to recombinant HAs from indicated influenza virus strains. Relative binding activity to each HA (numbers in each panel) was defined as the ratio of its area under curve (AUC) to that of the relevant 2010 vaccine strain (circle in each panel, assigned a value of 1).

    Article Snippet: Ninety-six–well ELISA plates (Greiner) were coated with IIV (2.7 µg/mL), recombinant HAs (Immune Technology, at 5 µg/mL), or recombinant NP (5 µg/mL).

    Techniques: Binding Assay, Activity Assay, Derivative Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Recombinant