iga elisas  (Thermo Fisher)


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    Structured Review

    Thermo Fisher iga elisas
    Iga Elisas, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga elisas/product/Thermo Fisher
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    iga elisas - by Bioz Stars, 2020-07
    93/100 stars

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    Incubation:

    Article Title: Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum
    Article Snippet: .. IgA ELISAs were washed and then incubated with strepavidin conjugated to horseradish peroxidase (SA-HRP, Pierce) prior to the final detection step. .. After washing, chromogenic substrate (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid; ABTS, Sigma, St Louis MO) was added, and the OD at 405nm was measured at 30 and 60 minutes using a Spectramax 190 (Molecular Devices, LLC) plate reader.

    other:

    Article Title: Natural Immunity to HIV is associated with Low BLyS/BAFF levels and low frequencies of innate marginal zone like CD1c+ B-cells in the genital tract
    Article Snippet: IgG and IgA recovery in eluates and presence within remaining supernatants were verified by performing human total IgG and IgA ELISAs (ThermoFisher).

    Article Title: Biochemical and Immunological Evaluation of Recombinant CS6-Derived Subunit Enterotoxigenic Escherichia coli Vaccine Candidates
    Article Snippet: Antigen (Ag)-specific IgG and IgA ELISAs were performed on Nunc MicroWell and Nunc MicroWell MaxiSorp 96-well plates, respectively (Thermo Scientific, Rochester, NY).

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  • 99
    Thermo Fisher anti tissue transglutaminase iga elisa kit
    The flow of participants through each stage of this randomised trial. P, Prednisolone tTg: tissue <t>transglutaminase;</t> <t>ELISA:</t> enzyme-linked immunosorbent assay; n: number; ESPGHAN: European Society for Paediatric Gastroenterology, Hepatology and Nutrition; GFD: gluten-free diet; GFD+P: gluten-free diet, plus prednisolone
    Anti Tissue Transglutaminase Iga Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tissue transglutaminase iga elisa kit/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti tissue transglutaminase iga elisa kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher iga elisa igg
    The cellular phenotype is attributable to reduced BACH2 protein expression ( a ) BACH2 protein expression in primary immune cells of patients and controls. Shown are representative flow cytometry plots with MFIs indicated (left panels) and cumulative BACH2 protein expression (right panels) from patients relative to controls. ( b ) Cumulative BACH2 mRNA expression from naïve B cells of patients and controls. ( c ) Representative immunoblot for Flag and Hsp70 from lysates of HEK293T cells transfected with empty vector (EV), Flag-tagged WT or mutant murine Bach2 (L24P or E786K, the murine equivalent of E788K). Shown are a representative blot (left) and cumulative quantifications from n = 5 experiments (right). ( d ) PRDM1 mRNA expression in naïve B cells from patients and healthy controls: cumulative data. ( e and f ) PRDM1 mRNA expression in CD4 + T lymphocytes of healthy controls and patients transfected with either control or BACH2 ( e ) and healthy donor CD4 + T lymphocytes transfected with control or BACH2 RNAi ( f ). ( g ) Plasmablast formation, <t>IgG</t> class switch recombination and <t>IgA</t> secretion in naïve healthy control B cells transfected with control RNAi or RNAi specific for BACH2 and activated in vitro as shown. Shown are representative flow cytometry examples and cumulative data (n = 5, 5 and 4 experiments, respectively). Bars show mean ± sem; *p
    Iga Elisa Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga elisa igg/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iga elisa igg - by Bioz Stars, 2020-07
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    93
    Thermo Fisher enzyme linked immunosorbent assay elisa immunoglobulin immunoglobulin ig class igg titers
    Immunoglobulin <t>(Ig)G2a/IgG1</t> and IgG2c/IgG1 titer ratios in the presence of analgesia. <t>IgG2a/IgG2c</t> response is suppressed in vaccinated BALB/c and C57BL/6 mice. Sera were analyzed by <t>ELISA</t> using irradiated VAX cells or irradiated CO92 cells as capture antigen at a concentration of 10 μg/mL. The ratios of IgG2a/IgG1 antibody titers was calculated from sera of ( A ) BALB/c mice against irradiated VAX cells; VAX compared to VAX + ACE, p = 0.001; VAX compared to VAX + MEL, p = 0.015; ( B ) BALB/c mice against irradiated CO92 cells; VAX compared to VAX + ACE, p = 0.013; ( C ) C57BL/6 mice against irradiated VAX cells. For C57BL/6 VAX + ACE mice anti-VAX titers, one mouse demonstrated an IgG2c/IgG1 ratio of 2.52; this mouse was included for statistical analyses but excluded for graphical representation. ( D ) C57BL/6 mice against irradiated CO92 cells; VAX compared to VAX + ACE, p = 0.021. For C57BL/6 VAX mice anti-CO92 titers, one mouse demonstrated an IgG2c/IgG1 ratio of 4.03; this mouse was included for statistical analyses but excluded for graphical representation. * denotes p
    Enzyme Linked Immunosorbent Assay Elisa Immunoglobulin Immunoglobulin Ig Class Igg Titers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme linked immunosorbent assay elisa immunoglobulin immunoglobulin ig class igg titers/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme linked immunosorbent assay elisa immunoglobulin immunoglobulin ig class igg titers - by Bioz Stars, 2020-07
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    The flow of participants through each stage of this randomised trial. P, Prednisolone tTg: tissue transglutaminase; ELISA: enzyme-linked immunosorbent assay; n: number; ESPGHAN: European Society for Paediatric Gastroenterology, Hepatology and Nutrition; GFD: gluten-free diet; GFD+P: gluten-free diet, plus prednisolone

    Journal: Cureus

    Article Title: Addition of a Short Course of Prednisolone to a Gluten-Free Diet vs. Gluten-Free Diet Alone in Recovery of Celiac Disease: A Pilot Randomized Controlled Trial

    doi: 10.7759/cureus.2118

    Figure Lengend Snippet: The flow of participants through each stage of this randomised trial. P, Prednisolone tTg: tissue transglutaminase; ELISA: enzyme-linked immunosorbent assay; n: number; ESPGHAN: European Society for Paediatric Gastroenterology, Hepatology and Nutrition; GFD: gluten-free diet; GFD+P: gluten-free diet, plus prednisolone

    Article Snippet: IgA anti-tTg antibody measurements were done by using a Thermo® iEMS™ microplate reader with an anti-tissue transglutaminase IgA ELISA kit (Thermo Scientific, Waltham, Massachusetts, USA).

    Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

    The cellular phenotype is attributable to reduced BACH2 protein expression ( a ) BACH2 protein expression in primary immune cells of patients and controls. Shown are representative flow cytometry plots with MFIs indicated (left panels) and cumulative BACH2 protein expression (right panels) from patients relative to controls. ( b ) Cumulative BACH2 mRNA expression from naïve B cells of patients and controls. ( c ) Representative immunoblot for Flag and Hsp70 from lysates of HEK293T cells transfected with empty vector (EV), Flag-tagged WT or mutant murine Bach2 (L24P or E786K, the murine equivalent of E788K). Shown are a representative blot (left) and cumulative quantifications from n = 5 experiments (right). ( d ) PRDM1 mRNA expression in naïve B cells from patients and healthy controls: cumulative data. ( e and f ) PRDM1 mRNA expression in CD4 + T lymphocytes of healthy controls and patients transfected with either control or BACH2 ( e ) and healthy donor CD4 + T lymphocytes transfected with control or BACH2 RNAi ( f ). ( g ) Plasmablast formation, IgG class switch recombination and IgA secretion in naïve healthy control B cells transfected with control RNAi or RNAi specific for BACH2 and activated in vitro as shown. Shown are representative flow cytometry examples and cumulative data (n = 5, 5 and 4 experiments, respectively). Bars show mean ± sem; *p

    Journal: Nature immunology

    Article Title: BACH2 immunodeficiency illustrates an association between super-enhancers and haploinsufficiency

    doi: 10.1038/ni.3753

    Figure Lengend Snippet: The cellular phenotype is attributable to reduced BACH2 protein expression ( a ) BACH2 protein expression in primary immune cells of patients and controls. Shown are representative flow cytometry plots with MFIs indicated (left panels) and cumulative BACH2 protein expression (right panels) from patients relative to controls. ( b ) Cumulative BACH2 mRNA expression from naïve B cells of patients and controls. ( c ) Representative immunoblot for Flag and Hsp70 from lysates of HEK293T cells transfected with empty vector (EV), Flag-tagged WT or mutant murine Bach2 (L24P or E786K, the murine equivalent of E788K). Shown are a representative blot (left) and cumulative quantifications from n = 5 experiments (right). ( d ) PRDM1 mRNA expression in naïve B cells from patients and healthy controls: cumulative data. ( e and f ) PRDM1 mRNA expression in CD4 + T lymphocytes of healthy controls and patients transfected with either control or BACH2 ( e ) and healthy donor CD4 + T lymphocytes transfected with control or BACH2 RNAi ( f ). ( g ) Plasmablast formation, IgG class switch recombination and IgA secretion in naïve healthy control B cells transfected with control RNAi or RNAi specific for BACH2 and activated in vitro as shown. Shown are representative flow cytometry examples and cumulative data (n = 5, 5 and 4 experiments, respectively). Bars show mean ± sem; *p

    Article Snippet: IgG and IGA ELISA IgG and IgA secretion was determined with the Ready-set-go total IgG and IgA kits (Thermofisher) according to manufacturer protocols.

    Techniques: Expressing, Flow Cytometry, Cytometry, Transfection, Plasmid Preparation, Mutagenesis, In Vitro

    Immunoglobulin (Ig)G2a/IgG1 and IgG2c/IgG1 titer ratios in the presence of analgesia. IgG2a/IgG2c response is suppressed in vaccinated BALB/c and C57BL/6 mice. Sera were analyzed by ELISA using irradiated VAX cells or irradiated CO92 cells as capture antigen at a concentration of 10 μg/mL. The ratios of IgG2a/IgG1 antibody titers was calculated from sera of ( A ) BALB/c mice against irradiated VAX cells; VAX compared to VAX + ACE, p = 0.001; VAX compared to VAX + MEL, p = 0.015; ( B ) BALB/c mice against irradiated CO92 cells; VAX compared to VAX + ACE, p = 0.013; ( C ) C57BL/6 mice against irradiated VAX cells. For C57BL/6 VAX + ACE mice anti-VAX titers, one mouse demonstrated an IgG2c/IgG1 ratio of 2.52; this mouse was included for statistical analyses but excluded for graphical representation. ( D ) C57BL/6 mice against irradiated CO92 cells; VAX compared to VAX + ACE, p = 0.021. For C57BL/6 VAX mice anti-CO92 titers, one mouse demonstrated an IgG2c/IgG1 ratio of 4.03; this mouse was included for statistical analyses but excluded for graphical representation. * denotes p

    Journal: Vaccines

    Article Title: The Use of Analgesics during Vaccination with a Live Attenuated Yersinia pestis Vaccine Alters the Resulting Immune Response in Mice

    doi: 10.3390/vaccines7040205

    Figure Lengend Snippet: Immunoglobulin (Ig)G2a/IgG1 and IgG2c/IgG1 titer ratios in the presence of analgesia. IgG2a/IgG2c response is suppressed in vaccinated BALB/c and C57BL/6 mice. Sera were analyzed by ELISA using irradiated VAX cells or irradiated CO92 cells as capture antigen at a concentration of 10 μg/mL. The ratios of IgG2a/IgG1 antibody titers was calculated from sera of ( A ) BALB/c mice against irradiated VAX cells; VAX compared to VAX + ACE, p = 0.001; VAX compared to VAX + MEL, p = 0.015; ( B ) BALB/c mice against irradiated CO92 cells; VAX compared to VAX + ACE, p = 0.013; ( C ) C57BL/6 mice against irradiated VAX cells. For C57BL/6 VAX + ACE mice anti-VAX titers, one mouse demonstrated an IgG2c/IgG1 ratio of 2.52; this mouse was included for statistical analyses but excluded for graphical representation. ( D ) C57BL/6 mice against irradiated CO92 cells; VAX compared to VAX + ACE, p = 0.021. For C57BL/6 VAX mice anti-CO92 titers, one mouse demonstrated an IgG2c/IgG1 ratio of 4.03; this mouse was included for statistical analyses but excluded for graphical representation. * denotes p

    Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) Immunoglobulin (Ig) class IgG titers (IgG, IgG1, IgG2a, or IgG2c) from vaccinated mice were determined by an ELISA performed in 96-well, Immulon 2 HB, round-bottom plates (ThermoFisher).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Irradiation, Concentration Assay