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Bethyl iga elisas
Iga Elisas, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iga elisas/product/Bethyl
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
iga elisas - by Bioz Stars, 2020-07
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Article Title: Immunization of Malaria-Preexposed Volunteers With PfSPZ Vaccine Elicits Long-Lived IgM Invasion-Inhibitory and Complement-Fixing Antibodies
Article Snippet: Purity of fractions was assessed using total human IgM and IgA ELISAs (Bethyl Laboratories) according to the manufacturer’s protocol.

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  • 90
    Bethyl human iga elisa kits
    Biological functions evoked through CD89 ligationin transgenic monocytes/macrophages. (A-B) <t>IgA</t> binding capacity of peritoneal macrophages from CD89 Tg mice. Human dimeric IgA1 (dIgA1), monomeric IgA (mIgA) and mouse dIgA plus mIgA were used a typical dose-response curve for a FACS-binding assay data set is shown in (A) representing the mean of fluorescence intensity of IgA-CD89 on cell surface, and in (B) showing the detection of IgA-CD89 interaction by <t>ELISA.(C)</t> CD89 cross-linking triggered ROS production: Cells were incubated with an anti-CD89 mAb (MIP8a) and cross-linked with goat anti-mouse IgG1 to induce CD89-mediated ROS production. As a negative control, cells were solely incubated with MIP8a. As a positive control, cells were incubated with IFNγ and LPS. Intracellular ROS levels are expressed as the average DCF fluorescence intensity. ***P
    Human Iga Elisa Kits, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human iga elisa kits/product/Bethyl
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human iga elisa kits - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    90
    Bethyl pig iga elisa quantitation kit
    Hematoxylin and eosin stains of various tissue sections and <t>IgA</t> levels in different samples from the infected and control pigs. (A) Hematoxylin and eosin staining of turbinate bone, trachea, hilar lymph nodes, and lung abscissions from M. hyopneumoniae -infected and control pigs. The black arrow indicates the pathological areas. The scale bar is shown in the lower right corner of each image. (B and C) Serum IgA and nasal swab IgA were detected every 5 days by <t>ELISA.</t> (D) BALF IgA concentrations in 300 ml lavage fluid, as detected by ELISA. Each histogram represents the average IgA level of four pigs in the infected group (gray) or the control group (black). A Mann-Whitney test was performed to analyze the statistical significance of the serum IgA levels on day 10 and day 15 between the infected group and the control group since the data did not fulfill the criteria of normality. Analysis of significance of the other data was performed using two-tailed t tests. *, P
    Pig Iga Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pig iga elisa quantitation kit/product/Bethyl
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pig iga elisa quantitation kit - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    Bethyl mouse iga enzyme linked immunosorbent assay elisa quantitation set
    Fecal concentration of secretory <t>IgA</t> before and after LAB administration. The fecal concentration of secretory IgA before and after administration of C59 or GG during experiment 2 was determined using <t>ELISA.</t> The white and gray bars represent the concentration of secretory IgA (μg/ml) before and after LAB administration, respectively. The data are shown as the least-squares means ± SE (n = 5 in each group).
    Mouse Iga Enzyme Linked Immunosorbent Assay Elisa Quantitation Set, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse iga enzyme linked immunosorbent assay elisa quantitation set/product/Bethyl
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse iga enzyme linked immunosorbent assay elisa quantitation set - by Bioz Stars, 2020-07
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    85
    Bethyl human igg iga igm elisa quantitation kit
    <t>IgG,</t> <t>IgM</t> and <t>IgA</t> antibody levels comparison to NTHi outer membrane proteins D, P6 and OMP26 in acute (top) and convalescent (bottom) sera of 9 children with NTHi AOM. Antibodies concentrations were summarized as geometric mean concentration with 95% confidence
    Human Igg Iga Igm Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human igg iga igm elisa quantitation kit/product/Bethyl
    Average 85 stars, based on 2 article reviews
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    human igg iga igm elisa quantitation kit - by Bioz Stars, 2020-07
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    Biological functions evoked through CD89 ligationin transgenic monocytes/macrophages. (A-B) IgA binding capacity of peritoneal macrophages from CD89 Tg mice. Human dimeric IgA1 (dIgA1), monomeric IgA (mIgA) and mouse dIgA plus mIgA were used a typical dose-response curve for a FACS-binding assay data set is shown in (A) representing the mean of fluorescence intensity of IgA-CD89 on cell surface, and in (B) showing the detection of IgA-CD89 interaction by ELISA.(C) CD89 cross-linking triggered ROS production: Cells were incubated with an anti-CD89 mAb (MIP8a) and cross-linked with goat anti-mouse IgG1 to induce CD89-mediated ROS production. As a negative control, cells were solely incubated with MIP8a. As a positive control, cells were incubated with IFNγ and LPS. Intracellular ROS levels are expressed as the average DCF fluorescence intensity. ***P

    Journal: PLoS ONE

    Article Title: Critical Role of Kupffer Cell CD89 Expression in Experimental IgA Nephropathy

    doi: 10.1371/journal.pone.0159426

    Figure Lengend Snippet: Biological functions evoked through CD89 ligationin transgenic monocytes/macrophages. (A-B) IgA binding capacity of peritoneal macrophages from CD89 Tg mice. Human dimeric IgA1 (dIgA1), monomeric IgA (mIgA) and mouse dIgA plus mIgA were used a typical dose-response curve for a FACS-binding assay data set is shown in (A) representing the mean of fluorescence intensity of IgA-CD89 on cell surface, and in (B) showing the detection of IgA-CD89 interaction by ELISA.(C) CD89 cross-linking triggered ROS production: Cells were incubated with an anti-CD89 mAb (MIP8a) and cross-linked with goat anti-mouse IgG1 to induce CD89-mediated ROS production. As a negative control, cells were solely incubated with MIP8a. As a positive control, cells were incubated with IFNγ and LPS. Intracellular ROS levels are expressed as the average DCF fluorescence intensity. ***P

    Article Snippet: The mouse IgA, IgG, and IgM or human IgA concentration in mouse serum were determined using mouse IgA, IgG, IgM or human IgA ELISA Kits (Bethyl lab), respectively, according to the manufacturer’s instructions.

    Techniques: Transgenic Assay, Binding Assay, Mouse Assay, FACS, Fluorescence, Enzyme-linked Immunosorbent Assay, Incubation, Negative Control, Positive Control

    Serum IgA levels in CD89 transgenic mice were substantially decreased while IgA production by B lymphocytes was unaffected. (A) Decreased serum IgA concentration in CD89 C57BL/6-Tg mice compared with WT mice. Serum from 12-, 24-, and 32-wk-old CD89 Tg mice (n = 4) and their littermate controls (WT; n = 4) was tested with a sandwich ELISA using a commercial mouse IgA detection kit. (B) There was no significant difference between the IgA expression of blood lymphocytes in CD89 C57BL/6-Tg mice compared with WT mice. Blood lymphocytes from 24-wk-old CD89 Tg mice and their littermate controls (WT) were isolated and lysed, and analyzed using Western blot. (C-D) Serum IgM (C) and IgG (D) concentrations from CD89 C57BL/6-Tg mice were unaffected when compared with WT mice. Serum from 12-, 24-, and 32-wk-old CD89 Tg mice (n = 4) and their littermate controls (WT, n = 4) was tested with a sandwich ELISA using mouse commercial IgM and IgG detection kits.

    Journal: PLoS ONE

    Article Title: Critical Role of Kupffer Cell CD89 Expression in Experimental IgA Nephropathy

    doi: 10.1371/journal.pone.0159426

    Figure Lengend Snippet: Serum IgA levels in CD89 transgenic mice were substantially decreased while IgA production by B lymphocytes was unaffected. (A) Decreased serum IgA concentration in CD89 C57BL/6-Tg mice compared with WT mice. Serum from 12-, 24-, and 32-wk-old CD89 Tg mice (n = 4) and their littermate controls (WT; n = 4) was tested with a sandwich ELISA using a commercial mouse IgA detection kit. (B) There was no significant difference between the IgA expression of blood lymphocytes in CD89 C57BL/6-Tg mice compared with WT mice. Blood lymphocytes from 24-wk-old CD89 Tg mice and their littermate controls (WT) were isolated and lysed, and analyzed using Western blot. (C-D) Serum IgM (C) and IgG (D) concentrations from CD89 C57BL/6-Tg mice were unaffected when compared with WT mice. Serum from 12-, 24-, and 32-wk-old CD89 Tg mice (n = 4) and their littermate controls (WT, n = 4) was tested with a sandwich ELISA using mouse commercial IgM and IgG detection kits.

    Article Snippet: The mouse IgA, IgG, and IgM or human IgA concentration in mouse serum were determined using mouse IgA, IgG, IgM or human IgA ELISA Kits (Bethyl lab), respectively, according to the manufacturer’s instructions.

    Techniques: Transgenic Assay, Mouse Assay, Concentration Assay, Sandwich ELISA, Expressing, Isolation, Western Blot

    Decreased clearance of IgAN patient IgA by CD89-expressing Kupffer cells. (A) IgA, purified from a normal donor and from a patient with IgAN, was stained with Cy3-anti-human IgA Ab, and subsequently incubated with Kupffer cells from CD89 transgenic mice at a concentration of 1 μg/mL and a temperature of 37°C. After 1-h-incubation, intracellular vesicles containing IgA were diminished when IgAN patient-derived IgA endocytosis was assessed and compared with normal human IgA. (B) sCD89-IgA complexes in cell supernatant of (A) were detected by ELISA using A3 and anti-mouse IgA mAb, using 1 μg/mL human IgA binding to 5 μg/mL recCD89 as an internal control. The sCD89-IgA complexes were detected in the Kupffer cell supernatant after 1h of incubation with either control IgA or patient IgA. (C) Decreased clearance of 10 μg IgAN patient-derived IgA in C57BL/6-Tg mice when compared to normal human IgA. A total of 10 μg of each of the proteins was injected into the tail vein of C57BL/6-Tg (n = 5 per group). At the indicated times after injection, sera were obtained and IgA levels were determined. IgA from IgAN patients was cleared less quickly in Tg mice compared with normal IgA. (D) Induction of mesangial IgA/sCD89 deposits by injecting patient IgA into 6-wk-old C57BL/6-Tg mice. Double staining by anti-human IgA and anti-CD89 in kidney 48 h after constant injection of patient IgA into C57BL/6-Tg mice(n = 4) is shown. Bar = 10μm.

    Journal: PLoS ONE

    Article Title: Critical Role of Kupffer Cell CD89 Expression in Experimental IgA Nephropathy

    doi: 10.1371/journal.pone.0159426

    Figure Lengend Snippet: Decreased clearance of IgAN patient IgA by CD89-expressing Kupffer cells. (A) IgA, purified from a normal donor and from a patient with IgAN, was stained with Cy3-anti-human IgA Ab, and subsequently incubated with Kupffer cells from CD89 transgenic mice at a concentration of 1 μg/mL and a temperature of 37°C. After 1-h-incubation, intracellular vesicles containing IgA were diminished when IgAN patient-derived IgA endocytosis was assessed and compared with normal human IgA. (B) sCD89-IgA complexes in cell supernatant of (A) were detected by ELISA using A3 and anti-mouse IgA mAb, using 1 μg/mL human IgA binding to 5 μg/mL recCD89 as an internal control. The sCD89-IgA complexes were detected in the Kupffer cell supernatant after 1h of incubation with either control IgA or patient IgA. (C) Decreased clearance of 10 μg IgAN patient-derived IgA in C57BL/6-Tg mice when compared to normal human IgA. A total of 10 μg of each of the proteins was injected into the tail vein of C57BL/6-Tg (n = 5 per group). At the indicated times after injection, sera were obtained and IgA levels were determined. IgA from IgAN patients was cleared less quickly in Tg mice compared with normal IgA. (D) Induction of mesangial IgA/sCD89 deposits by injecting patient IgA into 6-wk-old C57BL/6-Tg mice. Double staining by anti-human IgA and anti-CD89 in kidney 48 h after constant injection of patient IgA into C57BL/6-Tg mice(n = 4) is shown. Bar = 10μm.

    Article Snippet: The mouse IgA, IgG, and IgM or human IgA concentration in mouse serum were determined using mouse IgA, IgG, IgM or human IgA ELISA Kits (Bethyl lab), respectively, according to the manufacturer’s instructions.

    Techniques: Expressing, Purification, Staining, Incubation, Transgenic Assay, Mouse Assay, Concentration Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, Injection, Double Staining

    Hematoxylin and eosin stains of various tissue sections and IgA levels in different samples from the infected and control pigs. (A) Hematoxylin and eosin staining of turbinate bone, trachea, hilar lymph nodes, and lung abscissions from M. hyopneumoniae -infected and control pigs. The black arrow indicates the pathological areas. The scale bar is shown in the lower right corner of each image. (B and C) Serum IgA and nasal swab IgA were detected every 5 days by ELISA. (D) BALF IgA concentrations in 300 ml lavage fluid, as detected by ELISA. Each histogram represents the average IgA level of four pigs in the infected group (gray) or the control group (black). A Mann-Whitney test was performed to analyze the statistical significance of the serum IgA levels on day 10 and day 15 between the infected group and the control group since the data did not fulfill the criteria of normality. Analysis of significance of the other data was performed using two-tailed t tests. *, P

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 2 (TLR2) and TLR4 Mediate the IgA Immune Response Induced by Mycoplasma hyopneumoniae

    doi: 10.1128/IAI.00697-19

    Figure Lengend Snippet: Hematoxylin and eosin stains of various tissue sections and IgA levels in different samples from the infected and control pigs. (A) Hematoxylin and eosin staining of turbinate bone, trachea, hilar lymph nodes, and lung abscissions from M. hyopneumoniae -infected and control pigs. The black arrow indicates the pathological areas. The scale bar is shown in the lower right corner of each image. (B and C) Serum IgA and nasal swab IgA were detected every 5 days by ELISA. (D) BALF IgA concentrations in 300 ml lavage fluid, as detected by ELISA. Each histogram represents the average IgA level of four pigs in the infected group (gray) or the control group (black). A Mann-Whitney test was performed to analyze the statistical significance of the serum IgA levels on day 10 and day 15 between the infected group and the control group since the data did not fulfill the criteria of normality. Analysis of significance of the other data was performed using two-tailed t tests. *, P

    Article Snippet: The contents of IgA, IgG, and IgM in the peripheral blood serum and nasal swabs of pigs were detected with a pig IgA ELISA quantitation kit (Bethyl, TX, USA), pig IgG ELISA quantitation kit (Bethyl), and pig IgM ELISA quantitation kit (Bethyl).

    Techniques: Infection, Staining, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Two Tailed Test

    M. hyopneumoniae promotes cell coculture models to secrete IgA. (A and B) Mouse B cells were cocultured with mouse lung DCs (LDCs) or lung Mφ (LMφ) at different ratios, and IgA levels in the culture supernatants were analyzed by Western blotting (A) and ELISA (B) after stimulation for 6 days with 10 μg/ml M. hyopneumoniae whole-cell lysate. All experiments were repeated three times independently, and two-tailed t tests were performed to analyze the significance differences between the LDC/B cell and LMφ/B cell-cocultured groups. ***, P

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 2 (TLR2) and TLR4 Mediate the IgA Immune Response Induced by Mycoplasma hyopneumoniae

    doi: 10.1128/IAI.00697-19

    Figure Lengend Snippet: M. hyopneumoniae promotes cell coculture models to secrete IgA. (A and B) Mouse B cells were cocultured with mouse lung DCs (LDCs) or lung Mφ (LMφ) at different ratios, and IgA levels in the culture supernatants were analyzed by Western blotting (A) and ELISA (B) after stimulation for 6 days with 10 μg/ml M. hyopneumoniae whole-cell lysate. All experiments were repeated three times independently, and two-tailed t tests were performed to analyze the significance differences between the LDC/B cell and LMφ/B cell-cocultured groups. ***, P

    Article Snippet: The contents of IgA, IgG, and IgM in the peripheral blood serum and nasal swabs of pigs were detected with a pig IgA ELISA quantitation kit (Bethyl, TX, USA), pig IgG ELISA quantitation kit (Bethyl), and pig IgM ELISA quantitation kit (Bethyl).

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    Fecal concentration of secretory IgA before and after LAB administration. The fecal concentration of secretory IgA before and after administration of C59 or GG during experiment 2 was determined using ELISA. The white and gray bars represent the concentration of secretory IgA (μg/ml) before and after LAB administration, respectively. The data are shown as the least-squares means ± SE (n = 5 in each group).

    Journal: PLoS ONE

    Article Title: The distinct effects of orally administered Lactobacillus rhamnosus GG and Lactococcus lactis subsp. lactis C59 on gene expression in the murine small intestine

    doi: 10.1371/journal.pone.0188985

    Figure Lengend Snippet: Fecal concentration of secretory IgA before and after LAB administration. The fecal concentration of secretory IgA before and after administration of C59 or GG during experiment 2 was determined using ELISA. The white and gray bars represent the concentration of secretory IgA (μg/ml) before and after LAB administration, respectively. The data are shown as the least-squares means ± SE (n = 5 in each group).

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) Total IgA concentration in the fecal extracts or intestinal fluids was determined using a mouse IgA enzyme-linked immunosorbent assay (ELISA) quantitation set (Bethyl Laboratories, Inc., Montgomery, AL).

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    IgG, IgM and IgA antibody levels comparison to NTHi outer membrane proteins D, P6 and OMP26 in acute (top) and convalescent (bottom) sera of 9 children with NTHi AOM. Antibodies concentrations were summarized as geometric mean concentration with 95% confidence

    Journal: Vaccine

    Article Title: Antibody Response to Haemophilus influenzae Outer Membrane Protein D, P6, and OMP26 After Nasopharyngeal Colonization and Acute Otitis Media in Children

    doi: 10.1016/j.vaccine.2010.08.063

    Figure Lengend Snippet: IgG, IgM and IgA antibody levels comparison to NTHi outer membrane proteins D, P6 and OMP26 in acute (top) and convalescent (bottom) sera of 9 children with NTHi AOM. Antibodies concentrations were summarized as geometric mean concentration with 95% confidence

    Article Snippet: The levels of IgG, IgM and IgA in the reference serum were quantitatively measured by using a human IgG/IgA/IgM ELISA quantitation kit (Bethyl laboratories).

    Techniques: Concentration Assay

    Individual IgG, IgM and IgA antibody levels to NTHi outer membrane proteins D, P6 and OMP26 in acute and convalescent sera of 9 children with NTHi AOM.

    Journal: Vaccine

    Article Title: Antibody Response to Haemophilus influenzae Outer Membrane Protein D, P6, and OMP26 After Nasopharyngeal Colonization and Acute Otitis Media in Children

    doi: 10.1016/j.vaccine.2010.08.063

    Figure Lengend Snippet: Individual IgG, IgM and IgA antibody levels to NTHi outer membrane proteins D, P6 and OMP26 in acute and convalescent sera of 9 children with NTHi AOM.

    Article Snippet: The levels of IgG, IgM and IgA in the reference serum were quantitatively measured by using a human IgG/IgA/IgM ELISA quantitation kit (Bethyl laboratories).

    Techniques: