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Bethyl iga elisa quantitation kit
Effects of scFOS in the maternal diet on colostral and milk <t>IgA,</t> IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by <t>ELISA</t> assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05
Iga Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iga elisa quantitation kit/product/Bethyl
Average 88 stars, based on 16 article reviews
Price from $9.99 to $1999.99
iga elisa quantitation kit - by Bioz Stars, 2020-07
88/100 stars

Images

1) Product Images from "Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets"

Article Title: Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets

Journal: PLoS ONE

doi: 10.1371/journal.pone.0107508

Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05
Figure Legend Snippet: Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05

Techniques Used: Enzyme-linked Immunosorbent Assay

2) Product Images from "Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins"

Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2017.00953

Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P
Figure Legend Snippet: Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P

Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Comparison of mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins between visits with and without Mcat nasopharyngeal (NP) colonization in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 healthy visits with Mcat NP colonization positive and 131 healthy visits with Mcat NP colonization negative of 59 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 healthy visits with Mcat NP colonization positive and 140 healthy visits with Mcat NP colonization negative of 75 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P
Figure Legend Snippet: Comparison of mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins between visits with and without Mcat nasopharyngeal (NP) colonization in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 healthy visits with Mcat NP colonization positive and 131 healthy visits with Mcat NP colonization negative of 59 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 healthy visits with Mcat NP colonization positive and 140 healthy visits with Mcat NP colonization negative of 75 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins at healthy visits with Mcat nasopharyngeal (NP) colonization vs. acute otitis media (AOM) visits in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 Mcat NP colonization visits of 47 sOP children and 42 Mcat -caused AOM visits of 31 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 Mcat NP colonization visits of 61 NOP children and 43 Mcat -caused AOM visits of 37 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P
Figure Legend Snippet: Mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins at healthy visits with Mcat nasopharyngeal (NP) colonization vs. acute otitis media (AOM) visits in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 Mcat NP colonization visits of 47 sOP children and 42 Mcat -caused AOM visits of 31 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 Mcat NP colonization visits of 61 NOP children and 43 Mcat -caused AOM visits of 37 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

3) Product Images from "Construction of a Salmonella Gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens"

Article Title: Construction of a Salmonella Gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens

Journal: Veterinary Research

doi: 10.1186/1297-9716-43-44

The plasma IgG and intestinal sIgA levels in chickens against the outer membrane protein (OMP). A ) Plasma IgG levels against specific antigens were determined with the chicken IgG quantitative ELISA. B ) The sIgA levels in intestinal wash fluids against OMP antigen were quantified using the chicken IgA quantitative ELISA. Antibody levels were expressed as mean ± S.D. values for each week post-immunization. The values were considered statistically significant, if p values were ≤ 0.05 (* p ≤ 0.05). Group A, non-immunized control; group B, orally immunized; group C, immunized subcutaneously; group D, intramuscularly immunized.
Figure Legend Snippet: The plasma IgG and intestinal sIgA levels in chickens against the outer membrane protein (OMP). A ) Plasma IgG levels against specific antigens were determined with the chicken IgG quantitative ELISA. B ) The sIgA levels in intestinal wash fluids against OMP antigen were quantified using the chicken IgA quantitative ELISA. Antibody levels were expressed as mean ± S.D. values for each week post-immunization. The values were considered statistically significant, if p values were ≤ 0.05 (* p ≤ 0.05). Group A, non-immunized control; group B, orally immunized; group C, immunized subcutaneously; group D, intramuscularly immunized.

Techniques Used: Enzyme-linked Immunosorbent Assay, IF-P

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Enzyme-linked Immunosorbent Assay:

Article Title: Evaluating the effect of aromatherapy on a stress marker in healthy subjects
Article Snippet: .. Measurement of the salivary s-IgA level Absorbance (450 nm) was measured/quantified using an IgA ELISA Quantitation Kit® (Bethyl Laboratories) in which enzyme-linked immunosorbent assay (ELISA) was introduced as a principle, Varioskan Flash multi-functional microplate reader® (Thermo Scientific), and LP-PLATE manager 2013® (Thermo Fisher Scientific). .. Blood collection Blood at 5 mL was collected using a Venoject® II vacuum blood collection tube (Terumo Corporation) on the day of saliva collection.

Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins
Article Snippet: .. The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories). ..

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Article Snippet: .. The presence of plasma IgG and intestinal sIgA against the OMP was identified using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA), as described previously [ ]. .. Lymphocyte proliferation assay Lymphocyte proliferation assay was performed to determine lymphocyte activation and cell-mediated immune responses induced after vaccination [ ].

Article Title: Construction of a Salmonella Gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens
Article Snippet: .. The presence of plasma IgG and intestinal sIgA against the OMP was identified using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA). .. The wells of a Microlon® ELISA plate (Greiner Bio-One GmbH, Frickenhausen, Germany) were coated with 100 μL of OMP at a concentration of 0.2 mg/mL.

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Article Title: Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets
Article Snippet: .. Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA). ..

Article Title: Protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivated Salmonella Gallinarum vaccine candidate
Article Snippet: .. The plasma IgG and intestinal sIgA concentrations were quantified by an indirect enzyme-linked immunosorbent assay (ELISA) against the outer membrane protein (OMP) extracted from the JOL394 SG wild type strain [ ] using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA). .. The wells of a Microlon ELISA plate (Greiner Bio-One GmbH, Frickenhausen, Germany) were coated with 100 µL of OMP at a concentration of 0.2 mg/mL.

Concentration Assay:

Article Title: Characterisation of Early-Life Fecal Microbiota in Susceptible and Healthy Pigs to Post-Weaning Diarrhoea
Article Snippet: .. Colostral immunoglobulin G and A and TGF-B1 assays Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA). ..

Article Title: Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets
Article Snippet: .. Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA). ..

Quantitation Assay:

Article Title: Evaluating the effect of aromatherapy on a stress marker in healthy subjects
Article Snippet: .. Measurement of the salivary s-IgA level Absorbance (450 nm) was measured/quantified using an IgA ELISA Quantitation Kit® (Bethyl Laboratories) in which enzyme-linked immunosorbent assay (ELISA) was introduced as a principle, Varioskan Flash multi-functional microplate reader® (Thermo Scientific), and LP-PLATE manager 2013® (Thermo Fisher Scientific). .. Blood collection Blood at 5 mL was collected using a Venoject® II vacuum blood collection tube (Terumo Corporation) on the day of saliva collection.

Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins
Article Snippet: .. The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories). ..

Article Title: Salmonella enterica Serovar Enteritidis Ghosts Carrying the Escherichia coli Heat-Labile Enterotoxin B Subunit Are Capable of Inducing Enhanced Protective Immune Responses
Article Snippet: .. The titers of anti-OMP plasma IgG and intestinal sIgA antibodies were determined by using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA), as previously described ( ). .. To determine LTB-specific plasma IgG titers, purified LTB was used as the coating antigen.

Article Title: Development of a Biosafety Enhanced and Immunogenic Salmonella Enteritidis Ghost Using an Antibiotic Resistance Gene Free Plasmid Carrying a Bacteriophage Lysis System
Article Snippet: .. The presence of plasma IgG and intestinal sIgA against the OMP was identified using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA), as described previously [ ]. .. Lymphocyte proliferation assay Lymphocyte proliferation assay was performed to determine lymphocyte activation and cell-mediated immune responses induced after vaccination [ ].

Article Title: Construction of a Salmonella Gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens
Article Snippet: .. The presence of plasma IgG and intestinal sIgA against the OMP was identified using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA). .. The wells of a Microlon® ELISA plate (Greiner Bio-One GmbH, Frickenhausen, Germany) were coated with 100 μL of OMP at a concentration of 0.2 mg/mL.

Article Title: Characterisation of Early-Life Fecal Microbiota in Susceptible and Healthy Pigs to Post-Weaning Diarrhoea
Article Snippet: .. Colostral immunoglobulin G and A and TGF-B1 assays Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA). ..

Article Title: Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets
Article Snippet: .. Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA). ..

Article Title: Protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivated Salmonella Gallinarum vaccine candidate
Article Snippet: .. The plasma IgG and intestinal sIgA concentrations were quantified by an indirect enzyme-linked immunosorbent assay (ELISA) against the outer membrane protein (OMP) extracted from the JOL394 SG wild type strain [ ] using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA). .. The wells of a Microlon ELISA plate (Greiner Bio-One GmbH, Frickenhausen, Germany) were coated with 100 µL of OMP at a concentration of 0.2 mg/mL.

Indirect ELISA:

Article Title: Protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivated Salmonella Gallinarum vaccine candidate
Article Snippet: .. The plasma IgG and intestinal sIgA concentrations were quantified by an indirect enzyme-linked immunosorbent assay (ELISA) against the outer membrane protein (OMP) extracted from the JOL394 SG wild type strain [ ] using a chicken IgG and IgA ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA). .. The wells of a Microlon ELISA plate (Greiner Bio-One GmbH, Frickenhausen, Germany) were coated with 100 µL of OMP at a concentration of 0.2 mg/mL.

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    Bethyl iga elisa quantitation kit
    Effects of scFOS in the maternal diet on colostral and milk <t>IgA,</t> IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by <t>ELISA</t> assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05
    Iga Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga elisa quantitation kit/product/Bethyl
    Average 88 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    iga elisa quantitation kit - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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    Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05

    Journal: PLoS ONE

    Article Title: Maternal Short-Chain Fructooligosaccharide Supplementation Influences Intestinal Immune System Maturation in Piglets

    doi: 10.1371/journal.pone.0107508

    Figure Lengend Snippet: Effects of scFOS in the maternal diet on colostral and milk IgA, IgG and TGFβ1 concentrations. Concentrations of IgA, IgG and TGFβ1 in colostrum and milk of CTRL (open bars) and scFOS (filled bars) sows were analysed by ELISA assay. Bars are mean values ± SEM; n = 13–14 for colostrum; n = 17 for milks. * Significantly different between both groups, P ≤0.05; # tendency to be different between the two groups, 0.05

    Article Snippet: Concentration of Ig was quantified using swine IgG or IgA ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, Texas, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P

    Journal: Frontiers in Immunology

    Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins

    doi: 10.3389/fimmu.2017.00953

    Figure Lengend Snippet: Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P

    Article Snippet: The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Comparison of mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins between visits with and without Mcat nasopharyngeal (NP) colonization in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 healthy visits with Mcat NP colonization positive and 131 healthy visits with Mcat NP colonization negative of 59 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 healthy visits with Mcat NP colonization positive and 140 healthy visits with Mcat NP colonization negative of 75 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Journal: Frontiers in Immunology

    Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins

    doi: 10.3389/fimmu.2017.00953

    Figure Lengend Snippet: Comparison of mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins between visits with and without Mcat nasopharyngeal (NP) colonization in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 healthy visits with Mcat NP colonization positive and 131 healthy visits with Mcat NP colonization negative of 59 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 healthy visits with Mcat NP colonization positive and 140 healthy visits with Mcat NP colonization negative of 75 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Article Snippet: The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins at healthy visits with Mcat nasopharyngeal (NP) colonization vs. acute otitis media (AOM) visits in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 Mcat NP colonization visits of 47 sOP children and 42 Mcat -caused AOM visits of 31 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 Mcat NP colonization visits of 61 NOP children and 43 Mcat -caused AOM visits of 37 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Journal: Frontiers in Immunology

    Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins

    doi: 10.3389/fimmu.2017.00953

    Figure Lengend Snippet: Mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins at healthy visits with Mcat nasopharyngeal (NP) colonization vs. acute otitis media (AOM) visits in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 Mcat NP colonization visits of 47 sOP children and 42 Mcat -caused AOM visits of 31 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 Mcat NP colonization visits of 61 NOP children and 43 Mcat -caused AOM visits of 37 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Article Snippet: The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY