idk elisa kit  (Immundiagnostik AG)

Journal: Scientific Reports

Article Title: Bacterial DNAemia is associated with serum zonulin levels in older subjects

doi: 10.1038/s41598-021-90476-0

Figure Lengend Snippet: Linear regression and correlation analysis carried out between blood bacterial load and serum zonulin levels. Correlation has been assessed through Pearson’s ( r coefficient), Spearman’s (ρ), and Kendall’s (τ) analyses. Analyses were performed on the first (n = 43, panels A , B,C ) and the second (n = 42, panel D , E,F ) set of blood samples collected from older people. The second set of blood samples was collected approximately four months after the first draw from the same older volunteers. Panels A and D, tables showing correlation and regression indexes and p values. Panels B and E, linear regression plot; vertical dotted lines indicate the quartiles. Panels C and F, Tukey box plots showing levels of bacterial DNAema in blood samples grouped in quartiles based on zonulin level.

Article Snippet: Serum zonulin levels were quantified using IDK® Zonulin ELISA Kit (Immundiagnostik AG, Germany).

Techniques:

Journal: International Journal of Tryptophan Research : IJTR

Article Title: Plasma Kynurenine: A Promising Marker for the Assessment of Renal Functions

doi: 10.1177/11786469221102093

Figure Lengend Snippet: Correlation between kynurenine and kidney functions indicators: (a) kynurenine with eGFR calculated with CKD-EPI equation, (b) kynurenine with eGFR calculated with MDRD equation, (c) kynurenine with creatinine, and (d) kynurenine with urea.

Article Snippet: Kynurenine levels were measured by using a kynurenine ELISA kit (IDK® immundiagnostik).

Techniques:

Journal: International Journal of Tryptophan Research : IJTR

Article Title: Plasma Kynurenine: A Promising Marker for the Assessment of Renal Functions

doi: 10.1177/11786469221102093

Figure Lengend Snippet: Kynurenine level by stages of chronic kidney disease (3-5) and control.

Article Snippet: Kynurenine levels were measured by using a kynurenine ELISA kit (IDK® immundiagnostik).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19

doi: 10.1038/s41598-021-88356-8

Figure Lengend Snippet: Recombinant SARS-CoV-2 proteins. ( a ) 4–12% Bis–Tris gel loaded with 4 µg of SARS-CoV-2 spike protein (S), the RBD of spike protein (R), subunit 1 of the spike protein (S1), and ORF8 protein (O) stained with Coomassie brilliant blue R 250. ( b ) Immunoblot with 2 µg of Strep-tagged SARS-CoV-2 proteins loaded in the same manner as the Coomassie gel in ( a ), and detected with a Strep-Tactin-HRP conjugate. ( c ) Silver staining with 2 µg of SARS-CoV-2 S protein loaded in the same manner as the Coomassie gel in ( a ). All cropped lanes of different immunoblots or gels are divided by white space. Immunoblots and gels were only cut in width and lanes are shown in their full length.

Article Snippet: Enzyme-linked immunosorbent binding assay (ELISA)ELISA was performed with an IDK® anti-SARS-CoV-2 IgG ELISA kit from Immundiagnostik AG (Germany) strictly following the provided protocol.

Techniques: Recombinant, Staining, Silver Staining, Western Blot

Journal: Scientific Reports

Article Title: Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19

doi: 10.1038/s41598-021-88356-8

Figure Lengend Snippet: Anti-SARS-CoV-2 S1 protein IgG ELISA. Patient serum was tested for IgG isotype antibodies directed against subunit 1 of the SARS-CoV-2 spike protein (S1). Patients with a higher optical density (OD) measured at λ = 450 nm than the provided cut-off were labeled positive for IgG isotype antibodies directed against the S1 protein. Average ODs and standard deviations are shown in the bar chart. n = 3.

Article Snippet: Enzyme-linked immunosorbent binding assay (ELISA)ELISA was performed with an IDK® anti-SARS-CoV-2 IgG ELISA kit from Immundiagnostik AG (Germany) strictly following the provided protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Labeling