Journal: bioRxiv
Article Title: Olfactory fear conditioning biases olfactory stem cell receptor fate
doi: 10.1101/2023.02.23.529692
Figure Lengend Snippet: a . Schematic representation of the mouse main olfactory epithelium and olfactory bulb. MOE: main olfactory epithelium. OB: olfactory bulb. b . Timeline of olfactory fear conditioning and MOE collection. c . Experimental paradigms for olfactory fear conditioning groups. Mice in the paired condition received a footshock that co-terminated with odor presentation, while mice in the unpaired condition received a footshock 60 seconds after odor presentation. d . Schematic demonstrating the process by which cells of interest in the MOE were quantified. Epithelia from both M71-IRES-tauGFP +/+ and MOR23-IRES-tauGFP +/+ adult mice were cleared using the iDISCO+ tissue-clearing protocol. Samples were imaged on a light sheet microscope and analyzed using Imaris spot detection software. e . Images of the MOE before (left) and after (right) optical tissue clearing. f . The average number of M71 olfactory sensory neurons in a 350 3 um 3 cube of the epithelium in the propanol unpaired (light blue) and propanol paired (dark blue) conditions (Student’s unpaired t-test. Unpaired vs. paired P=0.3009. n=6,7.). g . Example images of M71 OSNs in zone 1 of cleared MOE from both the unpaired (left) and paired (middle) conditions. Example image of an MOE with the counted cells represented by colored dots (right). Each set of colors represents a distinct counting cube. Scale bar: 200um. h . Graph showing the differences between the average number of M71 OSNs in a 350 3 um 3 cube of epithelium of naive (gray), acetophenone unpaired (lighter green), and acetophenone paired (darker green) conditions in F0 and F1 (One-way ANOVA. P<0.0001. Tukey’s multiple comparisons. Naïve vs. F0 paired P<0.0001. F0 unpaired vs. paired P<0.0001. Naïve vs. F1 paired P<0.0001. F1 unpaired vs. paired P<0.0001. n=12,11,12,12,14.). i . Example images of MOR23 OSNs in zone 1 of cleared MOE from both the unpaired (left) and paired (middle) conditions. Example image of an MOE with the counted cells represented by colored dots (right). Scale bar: 200um. j . Graph showing the differences between the average number of MOR23 OSNs in a 350 3 um 3 cube of epithelium in naive (gray), lyral unpaired (lighter purple), and lyral paired (darker purple) conditions in F0 and F1 (One-way ANOVA. P<0.0001. Tukey’s multiple comparisons. Naïve vs. F0 paired P=0.0159. F0 unpaired vs. paired P<0.0001. F1 unpaired vs. paired P=0.0368. n=9,9,9,6,6.).
Article Snippet: C.W.L., E.C.B.J., H.S.L., A.K., and B.J.M. conducted iDISCO+ brain clearing and cell counts.
Techniques: Microscopy, Software
