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ide rabbit antibody  (Proteintech)
93
Proteintech ide rabbit antibody
Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
Ide Rabbit Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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639 beamlines 17 id 1 amx  (Brookhaven Instruments)
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Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
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beamline 28 id 1  (Brookhaven Instruments)
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Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
Beamline 28 Id 1, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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id 1 amx beamline  (Brookhaven Instruments)
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Brookhaven Instruments id 1 amx beamline
Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
Id 1 Amx Beamline, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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beamline 17 id 1 amx  (Brookhaven Instruments)
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Brookhaven Instruments beamline 17 id 1 amx
Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
Beamline 17 Id 1 Amx, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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id p2a4  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank id p2a4
Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
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anti ide  (Proteintech)
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Proteintech anti ide
Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
Anti Ide, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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national laboratory national synchrotron light source ii beamlines 17 id 1  (Brookhaven Instruments)
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Brookhaven Instruments national laboratory national synchrotron light source ii beamlines 17 id 1
Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
National Laboratory National Synchrotron Light Source Ii Beamlines 17 Id 1, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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id 1 beamline  (Brookhaven Instruments)
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Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
Id 1 Beamline, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glass filament needle 1 0 od 0 75 id  (World Precision Instruments)
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Experimental validation of <t>YOD1,</t> a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and <t>IDE</t> protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.
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Image Search Results


Experimental validation of YOD1, a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and IDE protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.

Journal: International Journal of Biological Sciences

Article Title: Young plasma-derived exosomes deliver YOD1 to induce M2 polarization and ameliorate osteoporosis via mTOR signaling pathways

doi: 10.7150/ijbs.138620

Figure Lengend Snippet: Experimental validation of YOD1, a candidate protein enriched in Y-Exos, in regulating osteogenic differentiation and macrophage polarization. (A) qPCR analysis of the effects of different concentrations of YOD1 (25 ng/mL and 50 ng/mL) on BMM polarization-related genes under non-inflammatory conditions, including TNF-α, IFN-γ, CD86, IL-4, Arg1, and CD206, n = 4. (B) Corresponding effects under LPS-induced inflammatory conditions, n = 4. (C) Western blotting analysis of YOD1, VCL, and IDE protein expression levels in plasma exosomes from donors of different ages, with TSG101 as the loading control, n = 3. (D-E) Flow cytometric analysis and quantification of CD86⁺ and CD206⁺ BMMs after YOD1 treatment under non-LPS and LPS conditions, n=3. (F) Western blotting analysis of YOD1 expression in BMMs and BMSCs after Y-Exos treatment, with GAPDH as the loading control, n = 3. (G) ALP staining and ARS staining evaluating the effect of YOD1 on BMSC osteogenic differentiation (scale bar = 100 μm), n = 3. (H) TRAP staining and F-actin ring staining evaluating the effect of YOD1 on BMM osteoclast differentiation (scale bar = 100 μm), n = 4. (I-J) Quantification of osteogenic differentiation (I: ALP activity and ARS staining) and osteoclast differentiation (J: osteoclast nuclei number and osteoclast area). Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.

Article Snippet: The primary and secondary antibodies are listed as follows: TSG101 Rabbit antibody (Proteintech, China, 28283-1-AP), CD63 Rabbit antibody (Proteintech, China, 25682-1-AP), CD9 Rabbit antibody (Proteintech, China, 20597-1-AP), Calnexin Rabbit antibody (Proteintech, China, 10427-2-AP), CD86 Rabbit antibody(Immunoway, USA, YM8023), CD206 Rabbit antibody (Immunoway, USA, YM8349), iNOS Rabbit antibody(Proteintech, China, 22226-1-AP), ARG1 Rabbit antibody (Abcam, China, ab96183), RUNX2 Rabbit antibody (Proteintech, China, 20700-1-AP), Osteocalcin Rabbit antibody(Servicebio, China, GB11233-100), ACTIN Mouse antibody(Proteintech, China, 66009-1-Ig), YOD1 Rabbit antibody (Proteintech, China, 25370-1-AP), IDE Rabbit antibody (Proteintech, China, 21728-1-AP), VCL Rabbit antibody (Proteintech, China, 26520-1-AP), GAPDH Rabbit antibody (BOSTER, China, A00227-1), ITCH Rabbit antibody (Proteintech, China, 20920-1-AP), LATS1 Rabbit antibody (Proteintech, China, 17049-1-AP), Phospho-YAP1 (Ser127) Rabbit antibody (Proteintech, China, 80694-2-RR), YAP1 Rabbit antibody (Proteintech, China, 13584-1-AP), Phospho-AKT Rabbit antibody (Proteintech, China, 29163-1-AP), AKT Rabbit antibody (Proteintech, China, 10176-2-AP), Phospho-mTOR Rabbit antibody(ZenBio, China, 381557), mTOR Rabbit antibody (HUA BIO, China, ET1608-5), NFATC1 Mouse antibody (Proteintech, China, 66963-1-Ig).

Techniques: Biomarker Discovery, Analysis, Western Blot, Expressing, Clinical Proteomics, Exosomes, Control, Staining, Activity Assay