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    Name:
    ICAM1 Monoclonal Antibody BE29G1
    Description:
    ICAM1 Monoclonal Antibody for IHC Flow IP
    Catalog Number:
    ma191180
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher icam1
    The rs26232 genotype is associated with RASF expression of <t>ICAM1,</t> MMP14 and IP-10. ( A ) Representative histogram showing ICAM-1 expression by RASFs of different rs26232 genotypes. ( B ) Expression of ICAM-1 protein is greater in RASFs of the CC compared to CT genotype (1.5-fold, p = 0.039). ( C ) ICAM1 relative gene expression is higher in CC compared to CT RASFs (2-fold, p = 0.044). ( D ) MMP14 relative gene expression is higher in CC compared to CT RASFs (1.6-fold, p = 0.021) ( E ) RASFs of CC genotype produce greater IP10 (CXCL10) compared to CT genotype (5-fold, p = 0.011). Each circle represents an individual donor. The black bar represents the mean. Statistical significance: * p
    ICAM1 Monoclonal Antibody for IHC Flow IP
    https://www.bioz.com/result/icam1/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    icam1 - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Association of the Rheumatoid Arthritis Severity Variant rs26232 with the Invasive Activity of Synovial Fibroblasts"

    Article Title: Association of the Rheumatoid Arthritis Severity Variant rs26232 with the Invasive Activity of Synovial Fibroblasts

    Journal: Cells

    doi: 10.3390/cells8101300

    The rs26232 genotype is associated with RASF expression of ICAM1, MMP14 and IP-10. ( A ) Representative histogram showing ICAM-1 expression by RASFs of different rs26232 genotypes. ( B ) Expression of ICAM-1 protein is greater in RASFs of the CC compared to CT genotype (1.5-fold, p = 0.039). ( C ) ICAM1 relative gene expression is higher in CC compared to CT RASFs (2-fold, p = 0.044). ( D ) MMP14 relative gene expression is higher in CC compared to CT RASFs (1.6-fold, p = 0.021) ( E ) RASFs of CC genotype produce greater IP10 (CXCL10) compared to CT genotype (5-fold, p = 0.011). Each circle represents an individual donor. The black bar represents the mean. Statistical significance: * p
    Figure Legend Snippet: The rs26232 genotype is associated with RASF expression of ICAM1, MMP14 and IP-10. ( A ) Representative histogram showing ICAM-1 expression by RASFs of different rs26232 genotypes. ( B ) Expression of ICAM-1 protein is greater in RASFs of the CC compared to CT genotype (1.5-fold, p = 0.039). ( C ) ICAM1 relative gene expression is higher in CC compared to CT RASFs (2-fold, p = 0.044). ( D ) MMP14 relative gene expression is higher in CC compared to CT RASFs (1.6-fold, p = 0.021) ( E ) RASFs of CC genotype produce greater IP10 (CXCL10) compared to CT genotype (5-fold, p = 0.011). Each circle represents an individual donor. The black bar represents the mean. Statistical significance: * p

    Techniques Used: Expressing

    2) Product Images from "Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿"

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿

    Journal:

    doi: 10.1128/MCB.01576-07

    TopoIIβ negatively regulates RA target genes. Real-time PCR analysis of RARβ, RIGI, HOXA1, and ICAM1 mRNA levels for the NB4 cell line (A) and the NB4-MR2 cell line (B), using the GAPDH gene as a reference gene. (A) mRNA levels for RARβ,
    Figure Legend Snippet: TopoIIβ negatively regulates RA target genes. Real-time PCR analysis of RARβ, RIGI, HOXA1, and ICAM1 mRNA levels for the NB4 cell line (A) and the NB4-MR2 cell line (B), using the GAPDH gene as a reference gene. (A) mRNA levels for RARβ,

    Techniques Used: Real-time Polymerase Chain Reaction

    3) Product Images from "Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿"

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿

    Journal:

    doi: 10.1128/MCB.01576-07

    TopoIIβ negatively regulates RA target genes. Real-time PCR analysis of RARβ, RIGI, HOXA1, and ICAM1 mRNA levels for the NB4 cell line (A) and the NB4-MR2 cell line (B), using the GAPDH gene as a reference gene. (A) mRNA levels for RARβ,
    Figure Legend Snippet: TopoIIβ negatively regulates RA target genes. Real-time PCR analysis of RARβ, RIGI, HOXA1, and ICAM1 mRNA levels for the NB4 cell line (A) and the NB4-MR2 cell line (B), using the GAPDH gene as a reference gene. (A) mRNA levels for RARβ,

    Techniques Used: Real-time Polymerase Chain Reaction

    4) Product Images from "Writing of H3K4Me3 overcomes epigenetic silencing in a sustained but context-dependent manner"

    Article Title: Writing of H3K4Me3 overcomes epigenetic silencing in a sustained but context-dependent manner

    Journal: Nature Communications

    doi: 10.1038/ncomms12284

    Local induction of H3K4me3 activates transcription of endogenous genes from promoter regions using zinc-finger proteins. ( a ) Relative messenger RNA (mRNA) expression of ICAM1 , RASSF1a and EpCAM in HEK293T, A549 and A2780 cells determined by qRT–PCR, induced by the indicated ZF fusion protein after retroviral transduction, the letter of each ZF corresponds to the same region where a gRNA binds at the promoter. ( b ) H3K4me3 ChIP–qPCR enrichment at the promoter region of EpCAM and RASSF1a in HEK293T, A549 and A2780 after retroviral transduction with ZF-PRDM9 and ZF-MutPRDM9 (two-tailed unpaired t -test, * P
    Figure Legend Snippet: Local induction of H3K4me3 activates transcription of endogenous genes from promoter regions using zinc-finger proteins. ( a ) Relative messenger RNA (mRNA) expression of ICAM1 , RASSF1a and EpCAM in HEK293T, A549 and A2780 cells determined by qRT–PCR, induced by the indicated ZF fusion protein after retroviral transduction, the letter of each ZF corresponds to the same region where a gRNA binds at the promoter. ( b ) H3K4me3 ChIP–qPCR enrichment at the promoter region of EpCAM and RASSF1a in HEK293T, A549 and A2780 after retroviral transduction with ZF-PRDM9 and ZF-MutPRDM9 (two-tailed unpaired t -test, * P

    Techniques Used: Expressing, Quantitative RT-PCR, Transduction, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Two Tailed Test

    Local induction of H3K4me3 activates transcription of endogenous genes from promoter regions using CRISPR–dCas9. ( a ) Schematic representation of the targeted genes and the (overlapping) locations where the ZFs and gRNAs bind (the letter or number of each region refers to the name of the ZF or gRNA, for the regions marked with * a ZF as well as a gRNA were designed). The yellow bars represent the location of the CpG islands. ( b ) Schematic of dCas9-VP64 targeting sense and antisense strands of DNA, and dCas9 and ZF fused to the epigenetic editor PRDM9 to locally induce H3K4me3. ( c ) Relative messenger RNA (mRNA) expression of ICAM1 , RASSF1a and EpCAM in HEK293T and A549 cells, and ( d ) PLOD2 in C33a cells, by the indicated dCas9 fusion protein co-transfected with a combination gRNAs targeted to each promoter region. ( n =3 independent experiments; error bars±s.d.).
    Figure Legend Snippet: Local induction of H3K4me3 activates transcription of endogenous genes from promoter regions using CRISPR–dCas9. ( a ) Schematic representation of the targeted genes and the (overlapping) locations where the ZFs and gRNAs bind (the letter or number of each region refers to the name of the ZF or gRNA, for the regions marked with * a ZF as well as a gRNA were designed). The yellow bars represent the location of the CpG islands. ( b ) Schematic of dCas9-VP64 targeting sense and antisense strands of DNA, and dCas9 and ZF fused to the epigenetic editor PRDM9 to locally induce H3K4me3. ( c ) Relative messenger RNA (mRNA) expression of ICAM1 , RASSF1a and EpCAM in HEK293T and A549 cells, and ( d ) PLOD2 in C33a cells, by the indicated dCas9 fusion protein co-transfected with a combination gRNAs targeted to each promoter region. ( n =3 independent experiments; error bars±s.d.).

    Techniques Used: CRISPR, Expressing, Transfection

    5) Product Images from "Interaction between ICAM1 in endothelial cells and LFA1 in T cells during the pathogenesis of experimental Parkinson's disease"

    Article Title: Interaction between ICAM1 in endothelial cells and LFA1 in T cells during the pathogenesis of experimental Parkinson's disease

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2020.8758

    ICAM1 is upregulated in brain endothelial cells and LFA1 is elevated in brain tissue T cells collected from MPTP-treated mice. (A) Representative images of ICAM1 in brain tissue endothelial cells of saline- or MPTP- treated mice. MPTP-treated mice exhibited increased levels of ICAM1. (B) Endothelial cells collected from the brains of MPTP-treated mice expressed significantly higher levels of ICAM1 4 days after MPTP injection (n=9). Experiments were performed in triplicate. **** P
    Figure Legend Snippet: ICAM1 is upregulated in brain endothelial cells and LFA1 is elevated in brain tissue T cells collected from MPTP-treated mice. (A) Representative images of ICAM1 in brain tissue endothelial cells of saline- or MPTP- treated mice. MPTP-treated mice exhibited increased levels of ICAM1. (B) Endothelial cells collected from the brains of MPTP-treated mice expressed significantly higher levels of ICAM1 4 days after MPTP injection (n=9). Experiments were performed in triplicate. **** P

    Techniques Used: Mouse Assay, Injection

    Blocking of ICAM1 or CD11a attenuates the severity of MPTP-induced PD in mice. (A) Blocking of ICAM1 or CD11a increased the levels of TH + cells at day 4 after MPTP injection. Mouse behaviour assessed by open field tests showed improved clinical presentation in those treated with ICAM1 or CD11a blockade at day 4 after MPTP injection, including (B) distance moved, (C) rears and (D) grooms. n=9. Experiments were performed in triplicate. ** P
    Figure Legend Snippet: Blocking of ICAM1 or CD11a attenuates the severity of MPTP-induced PD in mice. (A) Blocking of ICAM1 or CD11a increased the levels of TH + cells at day 4 after MPTP injection. Mouse behaviour assessed by open field tests showed improved clinical presentation in those treated with ICAM1 or CD11a blockade at day 4 after MPTP injection, including (B) distance moved, (C) rears and (D) grooms. n=9. Experiments were performed in triplicate. ** P

    Techniques Used: Blocking Assay, Mouse Assay, Injection

    Related Articles

    Countercurrent Chromatography:

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿
    Article Snippet: .. The reaction was carried out at 42°C for 50 min in the presence of SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified for RARβ and RIGI by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using hybridization probes. cDNA was amplified for ICAM1 and HOXA1, by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using primer sets as follows: for ICAM1, 5′ TGG CCC TCC ATA GAC ATG TGT 3′ (sense) and 5′ TGG CAT CCG TCA GGA AGT G 3′ (antisense); and for HOXA1, 5′ ACC CCG CCA GGA AAC G 3′ (sense) and 5′ GGC GAA GAG CTG GAC TTC TCT 3′ (antisense). .. Chromatin immunoprecipitation (ChIP) for analysis of TopoIIβ and histone 3 acetylation was carried out as follows.

    Amplification:

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿
    Article Snippet: .. The reaction was carried out at 42°C for 50 min in the presence of SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified for RARβ and RIGI by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using hybridization probes. cDNA was amplified for ICAM1 and HOXA1, by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using primer sets as follows: for ICAM1, 5′ TGG CCC TCC ATA GAC ATG TGT 3′ (sense) and 5′ TGG CAT CCG TCA GGA AGT G 3′ (antisense); and for HOXA1, 5′ ACC CCG CCA GGA AAC G 3′ (sense) and 5′ GGC GAA GAG CTG GAC TTC TCT 3′ (antisense). .. Chromatin immunoprecipitation (ChIP) for analysis of TopoIIβ and histone 3 acetylation was carried out as follows.

    Incubation:

    Article Title: Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis
    Article Snippet: .. 4.8 Immunofluorescence staining assay HUVECs and thoracic aortas were fixed with acetone-methanol (1:1, v/v) for 15 min at − 20 °C, washed in PBS, and blocked with 10% BSA in PBS with 0.1% Triton X-100 (PBST) for 30 min. HUVECs were incubated with anti-NRF2 (1:200), and the frozen thoracic aorta sections were incubated with anti-Ero1α (1:200) and anti-ICAM-1 (1:100) overnight at 4 °C, then washed with PBST and incubated with goat anti-rabbit Alexa Fluor 488 (1:500)/goat-anti-mouse Alexa Fluor 568 (1:500) for 1 h. The cells were counterstained with 0.05 mg/ml Hoechst stain for 10 min, rinsed with Hank's Balanced Salt Solution (HBSS, Gibco), and analyzed by confocal laser scanning microscopy (Zeiss, LSM710). .. 4.9 Protein sulfenylation determination HUVECs pre-treated with Hcy for 4 h were incubated with 5 mM dimedone for 1 h at 37 °C, washed with PBS and fixed with acetone-methanol (1:1, v/v) f or 10 min at − 20 °C, then washed again with PBS.

    Blocking Assay:

    Article Title: Intercellular Adhesion Molecule 1 (ICAM-1), but Not ICAM-2 and -3, Is Important for Dendritic Cell-Mediated Human Immunodeficiency Virus Type 1 Transmission ▿
    Article Snippet: .. For MAb blocking assays, DCs or other target cell types (2 × 105 ) were preincubated separately with purified MAbs (10 μg/ml; BD Biosciences, unless otherwise specified) of anti-ICAM-1 (clone HA58), anti-ICAM-2 (clone CBR-IC2/2; eBioscience), anti-ICAM-3 (clone TÜ41), and anti-LFA-1 (clone G43-25B) at room temperature for 0.5 h. A combination of different MAbs (10 μg/ml) was used when indicated. ..

    Article Title: Nurse-like cells promote CLL survival through LFA-3/CD2 interactions
    Article Snippet: .. Blocking antibodies anti-CD2 (clone TS2/18), anti-CD31 (clone HEC7), anti-ICAM-1 (clone W-CAM-1), anti-LFA-3 (clone TS2/9) and their relevant isotype controls were used at 10 μg/ml (Thermofisher, Villebon sur Yvette, France). .. Blocking antibodies anti-CXCL12 (Selleckchem, Houston, USA), anti-BAFF and anti-APRIL (clone 670820, R & D Systems, Minneapolis, USA) were used at 4 μM, 20 ng/ml and 1 μg/ml respectively.

    Purification:

    Article Title: Intercellular Adhesion Molecule 1 (ICAM-1), but Not ICAM-2 and -3, Is Important for Dendritic Cell-Mediated Human Immunodeficiency Virus Type 1 Transmission ▿
    Article Snippet: .. For MAb blocking assays, DCs or other target cell types (2 × 105 ) were preincubated separately with purified MAbs (10 μg/ml; BD Biosciences, unless otherwise specified) of anti-ICAM-1 (clone HA58), anti-ICAM-2 (clone CBR-IC2/2; eBioscience), anti-ICAM-3 (clone TÜ41), and anti-LFA-1 (clone G43-25B) at room temperature for 0.5 h. A combination of different MAbs (10 μg/ml) was used when indicated. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿
    Article Snippet: .. The reaction was carried out at 42°C for 50 min in the presence of SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified for RARβ and RIGI by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using hybridization probes. cDNA was amplified for ICAM1 and HOXA1, by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using primer sets as follows: for ICAM1, 5′ TGG CCC TCC ATA GAC ATG TGT 3′ (sense) and 5′ TGG CAT CCG TCA GGA AGT G 3′ (antisense); and for HOXA1, 5′ ACC CCG CCA GGA AAC G 3′ (sense) and 5′ GGC GAA GAG CTG GAC TTC TCT 3′ (antisense). .. Chromatin immunoprecipitation (ChIP) for analysis of TopoIIβ and histone 3 acetylation was carried out as follows.

    Article Title: Association of the Rheumatoid Arthritis Severity Variant rs26232 with the Invasive Activity of Synovial Fibroblasts
    Article Snippet: .. Relative quantitation (ΔΔCt method) of ICAM1, MMP14, CDH11, VCAM, and CTSK was carried out via TaqMan real-time PCR on an Applied Biosystems QuantStudio 7 Flex Real-Time PCR System using standard cycling conditions and 18s rRNA as an endogenous control. ..

    Quantitation Assay:

    Article Title: Association of the Rheumatoid Arthritis Severity Variant rs26232 with the Invasive Activity of Synovial Fibroblasts
    Article Snippet: .. Relative quantitation (ΔΔCt method) of ICAM1, MMP14, CDH11, VCAM, and CTSK was carried out via TaqMan real-time PCR on an Applied Biosystems QuantStudio 7 Flex Real-Time PCR System using standard cycling conditions and 18s rRNA as an endogenous control. ..

    Confocal Laser Scanning Microscopy:

    Article Title: Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis
    Article Snippet: .. 4.8 Immunofluorescence staining assay HUVECs and thoracic aortas were fixed with acetone-methanol (1:1, v/v) for 15 min at − 20 °C, washed in PBS, and blocked with 10% BSA in PBS with 0.1% Triton X-100 (PBST) for 30 min. HUVECs were incubated with anti-NRF2 (1:200), and the frozen thoracic aorta sections were incubated with anti-Ero1α (1:200) and anti-ICAM-1 (1:100) overnight at 4 °C, then washed with PBST and incubated with goat anti-rabbit Alexa Fluor 488 (1:500)/goat-anti-mouse Alexa Fluor 568 (1:500) for 1 h. The cells were counterstained with 0.05 mg/ml Hoechst stain for 10 min, rinsed with Hank's Balanced Salt Solution (HBSS, Gibco), and analyzed by confocal laser scanning microscopy (Zeiss, LSM710). .. 4.9 Protein sulfenylation determination HUVECs pre-treated with Hcy for 4 h were incubated with 5 mM dimedone for 1 h at 37 °C, washed with PBS and fixed with acetone-methanol (1:1, v/v) f or 10 min at − 20 °C, then washed again with PBS.

    Labeling:

    Article Title: Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity
    Article Snippet: .. After the washing procedure (PBS without Ca/Mg, Biochrom) cells were stained with ICAM-1 monoclonal antibody (BV2: Invitrogen, FITC labeled; U937 and primary macrophages: cell signaling, PE labeled). .. Analysis was performed using a flow cytometer (FACSCanto II, BD Biosciences, Heidelberg, Germany), collecting at least 20000 cells per sample.

    CTG Assay:

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿
    Article Snippet: .. The reaction was carried out at 42°C for 50 min in the presence of SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified for RARβ and RIGI by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using hybridization probes. cDNA was amplified for ICAM1 and HOXA1, by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using primer sets as follows: for ICAM1, 5′ TGG CCC TCC ATA GAC ATG TGT 3′ (sense) and 5′ TGG CAT CCG TCA GGA AGT G 3′ (antisense); and for HOXA1, 5′ ACC CCG CCA GGA AAC G 3′ (sense) and 5′ GGC GAA GAG CTG GAC TTC TCT 3′ (antisense). .. Chromatin immunoprecipitation (ChIP) for analysis of TopoIIβ and histone 3 acetylation was carried out as follows.

    Staining:

    Article Title: Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis
    Article Snippet: .. 4.8 Immunofluorescence staining assay HUVECs and thoracic aortas were fixed with acetone-methanol (1:1, v/v) for 15 min at − 20 °C, washed in PBS, and blocked with 10% BSA in PBS with 0.1% Triton X-100 (PBST) for 30 min. HUVECs were incubated with anti-NRF2 (1:200), and the frozen thoracic aorta sections were incubated with anti-Ero1α (1:200) and anti-ICAM-1 (1:100) overnight at 4 °C, then washed with PBST and incubated with goat anti-rabbit Alexa Fluor 488 (1:500)/goat-anti-mouse Alexa Fluor 568 (1:500) for 1 h. The cells were counterstained with 0.05 mg/ml Hoechst stain for 10 min, rinsed with Hank's Balanced Salt Solution (HBSS, Gibco), and analyzed by confocal laser scanning microscopy (Zeiss, LSM710). .. 4.9 Protein sulfenylation determination HUVECs pre-treated with Hcy for 4 h were incubated with 5 mM dimedone for 1 h at 37 °C, washed with PBS and fixed with acetone-methanol (1:1, v/v) f or 10 min at − 20 °C, then washed again with PBS.

    Article Title: Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity
    Article Snippet: .. After the washing procedure (PBS without Ca/Mg, Biochrom) cells were stained with ICAM-1 monoclonal antibody (BV2: Invitrogen, FITC labeled; U937 and primary macrophages: cell signaling, PE labeled). .. Analysis was performed using a flow cytometer (FACSCanto II, BD Biosciences, Heidelberg, Germany), collecting at least 20000 cells per sample.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿
    Article Snippet: .. The reaction was carried out at 42°C for 50 min in the presence of SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified for RARβ and RIGI by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using hybridization probes. cDNA was amplified for ICAM1 and HOXA1, by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using primer sets as follows: for ICAM1, 5′ TGG CCC TCC ATA GAC ATG TGT 3′ (sense) and 5′ TGG CAT CCG TCA GGA AGT G 3′ (antisense); and for HOXA1, 5′ ACC CCG CCA GGA AAC G 3′ (sense) and 5′ GGC GAA GAG CTG GAC TTC TCT 3′ (antisense). .. Chromatin immunoprecipitation (ChIP) for analysis of TopoIIβ and histone 3 acetylation was carried out as follows.

    Immunofluorescence:

    Article Title: Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis
    Article Snippet: .. 4.8 Immunofluorescence staining assay HUVECs and thoracic aortas were fixed with acetone-methanol (1:1, v/v) for 15 min at − 20 °C, washed in PBS, and blocked with 10% BSA in PBS with 0.1% Triton X-100 (PBST) for 30 min. HUVECs were incubated with anti-NRF2 (1:200), and the frozen thoracic aorta sections were incubated with anti-Ero1α (1:200) and anti-ICAM-1 (1:100) overnight at 4 °C, then washed with PBST and incubated with goat anti-rabbit Alexa Fluor 488 (1:500)/goat-anti-mouse Alexa Fluor 568 (1:500) for 1 h. The cells were counterstained with 0.05 mg/ml Hoechst stain for 10 min, rinsed with Hank's Balanced Salt Solution (HBSS, Gibco), and analyzed by confocal laser scanning microscopy (Zeiss, LSM710). .. 4.9 Protein sulfenylation determination HUVECs pre-treated with Hcy for 4 h were incubated with 5 mM dimedone for 1 h at 37 °C, washed with PBS and fixed with acetone-methanol (1:1, v/v) f or 10 min at − 20 °C, then washed again with PBS.

    Hybridization:

    Article Title: Topoisomerase II? Negatively Modulates Retinoic Acid Receptor ? Function: a Novel Mechanism of Retinoic Acid Resistance ▿
    Article Snippet: .. The reaction was carried out at 42°C for 50 min in the presence of SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified for RARβ and RIGI by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using hybridization probes. cDNA was amplified for ICAM1 and HOXA1, by real-time PCR analysis (ABI Prism7500; Applied Biosystems) using primer sets as follows: for ICAM1, 5′ TGG CCC TCC ATA GAC ATG TGT 3′ (sense) and 5′ TGG CAT CCG TCA GGA AGT G 3′ (antisense); and for HOXA1, 5′ ACC CCG CCA GGA AAC G 3′ (sense) and 5′ GGC GAA GAG CTG GAC TTC TCT 3′ (antisense). .. Chromatin immunoprecipitation (ChIP) for analysis of TopoIIβ and histone 3 acetylation was carried out as follows.

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    Thermo Fisher gene exp icam1 mm00516023 m1
    Model of chronic activation of PDGFRβ in a model of skin fibrosis. Chronic activation of PDGFRβ in a model of skin fibrosis promotes vascular activation ( <t>ICAM1</t> and ANGPT2 ) and Type I IFN-dependent inflammatory response (ISGs). PDGFRβ activation significantly increases the numbers of IFNβ-producing inflammatory monocytes recruited to the tissue. PDGF-BB-dependent functions of tissue injury, blood vessel maintenance, and monocyte/macrophage cell recruitment in the skin were found to be dependent on IFNAR signaling (highlighted in green) in this model of dermal fibrosis.
    Gene Exp Icam1 Mm00516023 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp icam1 mm00516023 m1/product/Thermo Fisher
    Average 99 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
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    88
    Thermo Fisher icam1 fc
    Representative images of actin fluorescence staining (red) highlighting U937 and EphA2ΔC-EGFP-U937 ( A ) as well as J774.1 and EphA2ΔC-EGFP-J774.1 ( B ) cell morphology when cultured on a coverslip surface with stripes of efnA1-Fc plus <t>ICAM1-Fc</t> and ICAM1-Fc alone as well as efnA1-Fc plus VCAM-1-Fc and VCAM-1-Fc alone. Arrows indicate the dense actin filament arch/ring or spots of high expression in (A) .
    Icam1 Fc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icam1 fc/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    icam1 fc - by Bioz Stars, 2020-09
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      Buy from Supplier

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    Model of chronic activation of PDGFRβ in a model of skin fibrosis. Chronic activation of PDGFRβ in a model of skin fibrosis promotes vascular activation ( ICAM1 and ANGPT2 ) and Type I IFN-dependent inflammatory response (ISGs). PDGFRβ activation significantly increases the numbers of IFNβ-producing inflammatory monocytes recruited to the tissue. PDGF-BB-dependent functions of tissue injury, blood vessel maintenance, and monocyte/macrophage cell recruitment in the skin were found to be dependent on IFNAR signaling (highlighted in green) in this model of dermal fibrosis.

    Journal: PLoS ONE

    Article Title: PDGF-BB Promotes Type I IFN-Dependent Vascular Alterations and Monocyte Recruitment in a Model of Dermal Fibrosis

    doi: 10.1371/journal.pone.0162758

    Figure Lengend Snippet: Model of chronic activation of PDGFRβ in a model of skin fibrosis. Chronic activation of PDGFRβ in a model of skin fibrosis promotes vascular activation ( ICAM1 and ANGPT2 ) and Type I IFN-dependent inflammatory response (ISGs). PDGFRβ activation significantly increases the numbers of IFNβ-producing inflammatory monocytes recruited to the tissue. PDGF-BB-dependent functions of tissue injury, blood vessel maintenance, and monocyte/macrophage cell recruitment in the skin were found to be dependent on IFNAR signaling (highlighted in green) in this model of dermal fibrosis.

    Article Snippet: The following primers were used for qPCR: Mm00434228_m1 (IL1B), Mm00446190_m1 (IL6), Mm00443258_m1 (TNF), Mm00441242_m1 (CCL2), Mm00487796_m1 (MX1), Mm00491265_m1 (RSAD2), Mm00516793_g1 (IRF7), Mm00459183_m1 (IFIH1/MDA1), Mm00545822_m1 (ANGPT2), Mm00516023_m1 (ICAM1), m01178820_m1(TGFB1), and Mm00435546_m1 (PDGFRB).

    Techniques: Activation Assay

    Changes in the expression of vascular adhesion markers in retinal vessels are difficult to resolve if measured in total retinal RNA. ( A ) Expression of VCAM-1 , ICAM-1 , P- and E-selectin mRNA was measured by real time RT-PCR in intact retinas of control (white bars) and diabetic (black bars) wt, ApoE −/− , TNFα −/− and ApoE −/− /TNFα −/− mice. No statistically significant differences were seen between genotypes or between retinas from control and diabetic mice.Results are normalized to the expression of the housekeeping control cyclophilin B. ( B ) VCAM-1 , ICAM-1 and E-selectin mRNA were measured in isolated retinal vessels from control (white) and diabetic (black) ApoE −/− mice. Values are normalized to the expression of cyclophilin B and GAPDH. * p

    Journal: PLoS ONE

    Article Title: Vascular Cellular Adhesion Molecule-1 (VCAM-1) Expression in Mice Retinal Vessels Is Affected by Both Hyperglycemia and Hyperlipidemia

    doi: 10.1371/journal.pone.0012699

    Figure Lengend Snippet: Changes in the expression of vascular adhesion markers in retinal vessels are difficult to resolve if measured in total retinal RNA. ( A ) Expression of VCAM-1 , ICAM-1 , P- and E-selectin mRNA was measured by real time RT-PCR in intact retinas of control (white bars) and diabetic (black bars) wt, ApoE −/− , TNFα −/− and ApoE −/− /TNFα −/− mice. No statistically significant differences were seen between genotypes or between retinas from control and diabetic mice.Results are normalized to the expression of the housekeeping control cyclophilin B. ( B ) VCAM-1 , ICAM-1 and E-selectin mRNA were measured in isolated retinal vessels from control (white) and diabetic (black) ApoE −/− mice. Values are normalized to the expression of cyclophilin B and GAPDH. * p

    Article Snippet: TaqMan assays were from Applied Biosystems (assays on demand); VCAM-1 (Mm01320970_m1), ICAM-1 (Mm00516023_m1), E-selectin (Mm00441278_m1), P-selectin (Mm00441295_m1), TNFα (Mm99999068_m1), IL-6 (Mm00446190_m1), IL-1β (Mm01336189_m1), IFNγ (Mm01168134_m1), Caspase 1 (Mm00438023_m1) and Ppib (Mm00478295_m1).

    Techniques: Expressing, Quantitative RT-PCR, Mouse Assay, Isolation

    In vivo inhibition of NFAT reduces the expression of OPN and ICAM-1 in retinal vessels. mRNA expression of (a) OPN and (b) ICAM-1 in isolated retinal vessels from BALB/c mice treated with STZ (60 mg/kg) or vehicle (citrate buffer). Mice received for 4 weeks daily i.p. injections of A-285222 (0.29 mg/kg for 2 weeks followed by 0.15 mg/kg until termination) or vehicle (saline). Expression levels were determined by quantitative RT-PCR and HPRT was used as endogenous control. N = 7-8 mice/group. Two-way ANOVA revealed a significant effect of A-285222 on OPN and ICAM-1 expression; ∗ P

    Journal: Journal of Diabetes Research

    Article Title: Nuclear Factor of Activated T Cells Is Activated in the Endothelium of Retinal Microvessels in Diabetic Mice

    doi: 10.1155/2015/428473

    Figure Lengend Snippet: In vivo inhibition of NFAT reduces the expression of OPN and ICAM-1 in retinal vessels. mRNA expression of (a) OPN and (b) ICAM-1 in isolated retinal vessels from BALB/c mice treated with STZ (60 mg/kg) or vehicle (citrate buffer). Mice received for 4 weeks daily i.p. injections of A-285222 (0.29 mg/kg for 2 weeks followed by 0.15 mg/kg until termination) or vehicle (saline). Expression levels were determined by quantitative RT-PCR and HPRT was used as endogenous control. N = 7-8 mice/group. Two-way ANOVA revealed a significant effect of A-285222 on OPN and ICAM-1 expression; ∗ P

    Article Snippet: For quantitative real time PCR, cDNA was synthesized using random hexamer primers and amplified on a 7900HT TaqMan system (Applied Biosystems, Carlsbad, CA) using TaqMan Gene Expression assays for Tau (Mm00521988_m1), GFAP (Mm01253033_m1), OPN (Mm00436767_m1), ICAM-1 (Mm00516023_m1) with 18S (Hs999999021_s1), HPRT (Mm00446968_m1), Cyclophilin B (Mm00478295_m), and GAPDH (4352339E) as endogenous controls.

    Techniques: In Vivo, Inhibition, Expressing, Isolation, Mouse Assay, Quantitative RT-PCR

    Representative images of actin fluorescence staining (red) highlighting U937 and EphA2ΔC-EGFP-U937 ( A ) as well as J774.1 and EphA2ΔC-EGFP-J774.1 ( B ) cell morphology when cultured on a coverslip surface with stripes of efnA1-Fc plus ICAM1-Fc and ICAM1-Fc alone as well as efnA1-Fc plus VCAM-1-Fc and VCAM-1-Fc alone. Arrows indicate the dense actin filament arch/ring or spots of high expression in (A) .

    Journal: Cell Adhesion & Migration

    Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

    doi: 10.1080/19336918.2015.1107693

    Figure Lengend Snippet: Representative images of actin fluorescence staining (red) highlighting U937 and EphA2ΔC-EGFP-U937 ( A ) as well as J774.1 and EphA2ΔC-EGFP-J774.1 ( B ) cell morphology when cultured on a coverslip surface with stripes of efnA1-Fc plus ICAM1-Fc and ICAM1-Fc alone as well as efnA1-Fc plus VCAM-1-Fc and VCAM-1-Fc alone. Arrows indicate the dense actin filament arch/ring or spots of high expression in (A) .

    Article Snippet: For precipitation, 800 µg of the cell extracts were incubated overnight at 4˚C or for 2 hrs at room temperature with 2 µg of ICAM1-Fc or VCAM1-Fc, followed by the addition of 20 µL of protein A/G-magnetic beads (Thermo Fisher Scientific; 88802) for 1 hr at room temperature.

    Techniques: Fluorescence, Staining, Cell Culture, Expressing

    U937 and EphA2ΔC-EGFP-U937 cells preferentially occupy the efnA1-Fc plus integrin ligand protein-coated surfaces. ( A ) Typical phase contrast micrographs showing cells cultured on a coverslip surface on which efnA1-Fc and the indicated integrin ligand were adsorbed in stripes (approximately 0.48-mm in width) and overall, respectively. ( B ) Representative high magnification phase contrast micrographs showing U937 and EphA2ΔC-EGFP-U937 cells cultured on a coverslip surface in stripes of efnA1-Fc plus ICAM1-Fc and ICAM1-Fc only. ( C ) Quantified cell densities in the stripes of efnA1-Fc plus integrin ligand compared to integrin ligand only surfaces. The results from 3 independent experiments are shown. Data are presented as the means ± SD. ** P

    Journal: Cell Adhesion & Migration

    Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

    doi: 10.1080/19336918.2015.1107693

    Figure Lengend Snippet: U937 and EphA2ΔC-EGFP-U937 cells preferentially occupy the efnA1-Fc plus integrin ligand protein-coated surfaces. ( A ) Typical phase contrast micrographs showing cells cultured on a coverslip surface on which efnA1-Fc and the indicated integrin ligand were adsorbed in stripes (approximately 0.48-mm in width) and overall, respectively. ( B ) Representative high magnification phase contrast micrographs showing U937 and EphA2ΔC-EGFP-U937 cells cultured on a coverslip surface in stripes of efnA1-Fc plus ICAM1-Fc and ICAM1-Fc only. ( C ) Quantified cell densities in the stripes of efnA1-Fc plus integrin ligand compared to integrin ligand only surfaces. The results from 3 independent experiments are shown. Data are presented as the means ± SD. ** P

    Article Snippet: For precipitation, 800 µg of the cell extracts were incubated overnight at 4˚C or for 2 hrs at room temperature with 2 µg of ICAM1-Fc or VCAM1-Fc, followed by the addition of 20 µL of protein A/G-magnetic beads (Thermo Fisher Scientific; 88802) for 1 hr at room temperature.

    Techniques: Cell Culture

    Molecular association of EphA2 with the β2 integrin/ICAM1 ( A ) and β2 integrin/VCAM1 complexes ( B ) in U937 and EphA2ΔC-EGFP-U937 cells.

    Journal: Cell Adhesion & Migration

    Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

    doi: 10.1080/19336918.2015.1107693

    Figure Lengend Snippet: Molecular association of EphA2 with the β2 integrin/ICAM1 ( A ) and β2 integrin/VCAM1 complexes ( B ) in U937 and EphA2ΔC-EGFP-U937 cells.

    Article Snippet: For precipitation, 800 µg of the cell extracts were incubated overnight at 4˚C or for 2 hrs at room temperature with 2 µg of ICAM1-Fc or VCAM1-Fc, followed by the addition of 20 µL of protein A/G-magnetic beads (Thermo Fisher Scientific; 88802) for 1 hr at room temperature.

    Techniques:

    J774.1 and EphA2ΔC-EGFP-J774.1 cells preferentially occupied the efnA1-Fc plus integrin ligand protein-coated regions rather than the integrin ligand only surface. ( A ) Typical phase contrast micrographs showing cells cultured on a coverslip surface on which efnA1-Fc and the indicated integrin ligand were adsorbed in stripes (approximately 0.48-mm in width) and overall, respectively. ( B ) Representative high magnification phase contrast micrographs showing cells cultured on a coverslip surface in stripes of efnA1-Fc plus ICAM1-Fc and ICAM1-Fc only. ( C ) Quantified cell densities in stripes of efnA1-Fc plus each integrin ligand. The results from 3 independent experiments are shown. Data are presented as the means ± SD. * P

    Journal: Cell Adhesion & Migration

    Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

    doi: 10.1080/19336918.2015.1107693

    Figure Lengend Snippet: J774.1 and EphA2ΔC-EGFP-J774.1 cells preferentially occupied the efnA1-Fc plus integrin ligand protein-coated regions rather than the integrin ligand only surface. ( A ) Typical phase contrast micrographs showing cells cultured on a coverslip surface on which efnA1-Fc and the indicated integrin ligand were adsorbed in stripes (approximately 0.48-mm in width) and overall, respectively. ( B ) Representative high magnification phase contrast micrographs showing cells cultured on a coverslip surface in stripes of efnA1-Fc plus ICAM1-Fc and ICAM1-Fc only. ( C ) Quantified cell densities in stripes of efnA1-Fc plus each integrin ligand. The results from 3 independent experiments are shown. Data are presented as the means ± SD. * P

    Article Snippet: For precipitation, 800 µg of the cell extracts were incubated overnight at 4˚C or for 2 hrs at room temperature with 2 µg of ICAM1-Fc or VCAM1-Fc, followed by the addition of 20 µL of protein A/G-magnetic beads (Thermo Fisher Scientific; 88802) for 1 hr at room temperature.

    Techniques: Cell Culture