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US Biological Life Sciences icam 1
<t>ICAM-1</t> concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Icam 1, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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icam 1 - by Bioz Stars, 2020-09
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1) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

2) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

3) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

4) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

5) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

6) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

7) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

8) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

9) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

10) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

11) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

12) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

13) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

14) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

15) Product Images from "Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery"

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

Journal: Journal of Proteome Research

doi: 10.1021/pr800904z

ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
Figure Legend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

Techniques Used: Concentration Assay

Related Articles

Biomarker Assay:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Independent Validation of Biomarker Candidates On the basis of the ELISA results, ICAM-1 and BCAM were chosen for further validation in an independent set of human serum samples. ..

Cell Culture:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Abbreviations: SILAP, stable isotope labeled proteome; MS/MS, tandem mass spectrometry; SILAC, stable isotope labeling by amino acids in cell culture; IEF, isoelectric focusing; 2D-LC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; SCX, strong cation exchange; CAPAN-2, Human Caucasian, pancreas, adenocarcinoma cells; KEGG, Kyoto Encyclopedia of Genes and Genomes; GI, GenBank Geninfo Identifier; GO, Gene Ontology; MMP-7, matrix metalloproteinase-7; CK18, cytokeratin 18; ICAM-1, intercellular adhesion molecule 1; BCAM, basal cell adhesion molecule; ELISA, enzyme-linked immunosorbent assay. .. Supported by NIH grants P30ES013508, P30CA16520, RO195586, R25CA101871, and U54RR023567, an Institute for Translational Medicine and Therapeutics Research Fellowship, and the Foundation for Digestive Health and Nutrition Bernard L. Schwartz Designated Research Award in Pancreatic Cancer.

Two-dimensional Liquid Chromatography:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Abbreviations: SILAP, stable isotope labeled proteome; MS/MS, tandem mass spectrometry; SILAC, stable isotope labeling by amino acids in cell culture; IEF, isoelectric focusing; 2D-LC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; SCX, strong cation exchange; CAPAN-2, Human Caucasian, pancreas, adenocarcinoma cells; KEGG, Kyoto Encyclopedia of Genes and Genomes; GI, GenBank Geninfo Identifier; GO, Gene Ontology; MMP-7, matrix metalloproteinase-7; CK18, cytokeratin 18; ICAM-1, intercellular adhesion molecule 1; BCAM, basal cell adhesion molecule; ELISA, enzyme-linked immunosorbent assay. .. Supported by NIH grants P30ES013508, P30CA16520, RO195586, R25CA101871, and U54RR023567, an Institute for Translational Medicine and Therapeutics Research Fellowship, and the Foundation for Digestive Health and Nutrition Bernard L. Schwartz Designated Research Award in Pancreatic Cancer.

Enzyme-linked Immunosorbent Assay:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Abbreviations: SILAP, stable isotope labeled proteome; MS/MS, tandem mass spectrometry; SILAC, stable isotope labeling by amino acids in cell culture; IEF, isoelectric focusing; 2D-LC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; SCX, strong cation exchange; CAPAN-2, Human Caucasian, pancreas, adenocarcinoma cells; KEGG, Kyoto Encyclopedia of Genes and Genomes; GI, GenBank Geninfo Identifier; GO, Gene Ontology; MMP-7, matrix metalloproteinase-7; CK18, cytokeratin 18; ICAM-1, intercellular adhesion molecule 1; BCAM, basal cell adhesion molecule; ELISA, enzyme-linked immunosorbent assay. .. Supported by NIH grants P30ES013508, P30CA16520, RO195586, R25CA101871, and U54RR023567, an Institute for Translational Medicine and Therapeutics Research Fellowship, and the Foundation for Digestive Health and Nutrition Bernard L. Schwartz Designated Research Award in Pancreatic Cancer.

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. MMP-7 (R & D Systems, Minneapolis, MN), ICAM-1 (US Biological, Swampscott, MA) and BCAM (R & D Systems, Minneapolis, MN) ELISA kits were purchased and used according to manufacturer’s instructions. ..

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Absolute quantitation by ELISA for ICAM-1 and BCAM confirmed the differences uncovered by the SILAP standard method, and demonstrates the sensitivity gain afforded by this method (Figures – ). ..

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Independent Validation of Biomarker Candidates On the basis of the ELISA results, ICAM-1 and BCAM were chosen for further validation in an independent set of human serum samples. ..

Concentration Assay:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. BCAM concentration was 2904 pg/mL, while the ICAM-1 concentration was 17 ng/mL. .. It is noteworthy the concentrations of BCAM and MMP-7 were significantly greater in the SILAP standard than in human serum, whereas ICAM-1 was found at a much lower concentration in the SILAP standard.

Quantitation Assay:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Absolute quantitation by ELISA for ICAM-1 and BCAM confirmed the differences uncovered by the SILAP standard method, and demonstrates the sensitivity gain afforded by this method (Figures – ). ..

other:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: Student’s t -tests were performed to determine if levels of ICAM-1 or BCAM were associated with patient gender or tumor resectability (Table ).

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: ICAM-1 and BCAM levels were significantly elevated in these cancer samples when compared with controls (Figure and Figure , respectively).

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: Both ICAM-1 and BCAM concentrations were significantly elevated in the individual cancer serum samples compared with control samples (Figure and Figure , respectively).

Labeling:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Abbreviations: SILAP, stable isotope labeled proteome; MS/MS, tandem mass spectrometry; SILAC, stable isotope labeling by amino acids in cell culture; IEF, isoelectric focusing; 2D-LC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; SCX, strong cation exchange; CAPAN-2, Human Caucasian, pancreas, adenocarcinoma cells; KEGG, Kyoto Encyclopedia of Genes and Genomes; GI, GenBank Geninfo Identifier; GO, Gene Ontology; MMP-7, matrix metalloproteinase-7; CK18, cytokeratin 18; ICAM-1, intercellular adhesion molecule 1; BCAM, basal cell adhesion molecule; ELISA, enzyme-linked immunosorbent assay. .. Supported by NIH grants P30ES013508, P30CA16520, RO195586, R25CA101871, and U54RR023567, an Institute for Translational Medicine and Therapeutics Research Fellowship, and the Foundation for Digestive Health and Nutrition Bernard L. Schwartz Designated Research Award in Pancreatic Cancer.

Mass Spectrometry:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Abbreviations: SILAP, stable isotope labeled proteome; MS/MS, tandem mass spectrometry; SILAC, stable isotope labeling by amino acids in cell culture; IEF, isoelectric focusing; 2D-LC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; SCX, strong cation exchange; CAPAN-2, Human Caucasian, pancreas, adenocarcinoma cells; KEGG, Kyoto Encyclopedia of Genes and Genomes; GI, GenBank Geninfo Identifier; GO, Gene Ontology; MMP-7, matrix metalloproteinase-7; CK18, cytokeratin 18; ICAM-1, intercellular adhesion molecule 1; BCAM, basal cell adhesion molecule; ELISA, enzyme-linked immunosorbent assay. .. Supported by NIH grants P30ES013508, P30CA16520, RO195586, R25CA101871, and U54RR023567, an Institute for Translational Medicine and Therapeutics Research Fellowship, and the Foundation for Digestive Health and Nutrition Bernard L. Schwartz Designated Research Award in Pancreatic Cancer.

Tandem Mass Spectroscopy:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Abbreviations: SILAP, stable isotope labeled proteome; MS/MS, tandem mass spectrometry; SILAC, stable isotope labeling by amino acids in cell culture; IEF, isoelectric focusing; 2D-LC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; SCX, strong cation exchange; CAPAN-2, Human Caucasian, pancreas, adenocarcinoma cells; KEGG, Kyoto Encyclopedia of Genes and Genomes; GI, GenBank Geninfo Identifier; GO, Gene Ontology; MMP-7, matrix metalloproteinase-7; CK18, cytokeratin 18; ICAM-1, intercellular adhesion molecule 1; BCAM, basal cell adhesion molecule; ELISA, enzyme-linked immunosorbent assay. .. Supported by NIH grants P30ES013508, P30CA16520, RO195586, R25CA101871, and U54RR023567, an Institute for Translational Medicine and Therapeutics Research Fellowship, and the Foundation for Digestive Health and Nutrition Bernard L. Schwartz Designated Research Award in Pancreatic Cancer.

Electrofocusing:

Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery
Article Snippet: .. Abbreviations: SILAP, stable isotope labeled proteome; MS/MS, tandem mass spectrometry; SILAC, stable isotope labeling by amino acids in cell culture; IEF, isoelectric focusing; 2D-LC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; SCX, strong cation exchange; CAPAN-2, Human Caucasian, pancreas, adenocarcinoma cells; KEGG, Kyoto Encyclopedia of Genes and Genomes; GI, GenBank Geninfo Identifier; GO, Gene Ontology; MMP-7, matrix metalloproteinase-7; CK18, cytokeratin 18; ICAM-1, intercellular adhesion molecule 1; BCAM, basal cell adhesion molecule; ELISA, enzyme-linked immunosorbent assay. .. Supported by NIH grants P30ES013508, P30CA16520, RO195586, R25CA101871, and U54RR023567, an Institute for Translational Medicine and Therapeutics Research Fellowship, and the Foundation for Digestive Health and Nutrition Bernard L. Schwartz Designated Research Award in Pancreatic Cancer.

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    US Biological Life Sciences icam 1
    <t>ICAM-1</t> concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.
    Icam 1, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icam 1/product/US Biological Life Sciences
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    icam 1 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    89
    US Biological Life Sciences mouse monoclonal anti icam 1
    Blocking <t>ICAM-1</t> and CD11a inhibits monocyte-EC adhesion. HUVECs and monocytes were treated with 4 mM AA or 8 mM BHB for 24 h. Following treatment, HUVEC monolayers were treated with 5 mg/ml mouse IgG antibody or 5 mg/ml ICAM-1 blocking antibody for 30
    Mouse Monoclonal Anti Icam 1, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti icam 1/product/US Biological Life Sciences
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti icam 1 - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results


    ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

    Journal: Journal of Proteome Research

    Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

    doi: 10.1021/pr800904z

    Figure Lengend Snippet: ICAM-1 concentrations in the six cancer and six control serum samples studied. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

    Article Snippet: Absolute quantitation by ELISA for ICAM-1 and BCAM confirmed the differences uncovered by the SILAP standard method, and demonstrates the sensitivity gain afforded by this method (Figures – ).

    Techniques: Concentration Assay

    ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

    Journal: Journal of Proteome Research

    Article Title: Stable Isotope Dilution Multidimensional Liquid Chromatography-Tandem Mass Spectrometry for Pancreatic Cancer Serum Biomarker Discovery

    doi: 10.1021/pr800904z

    Figure Lengend Snippet: ICAM-1 concentrations in ten cancer and ten control serum samples from an independent cohort. Mean ICAM-1 concentration in cancer sera was significantly greater than in controls.

    Article Snippet: Absolute quantitation by ELISA for ICAM-1 and BCAM confirmed the differences uncovered by the SILAP standard method, and demonstrates the sensitivity gain afforded by this method (Figures – ).

    Techniques: Concentration Assay

    Blocking ICAM-1 and CD11a inhibits monocyte-EC adhesion. HUVECs and monocytes were treated with 4 mM AA or 8 mM BHB for 24 h. Following treatment, HUVEC monolayers were treated with 5 mg/ml mouse IgG antibody or 5 mg/ml ICAM-1 blocking antibody for 30

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells

    doi: 10.1152/ajpendo.00038.2011

    Figure Lengend Snippet: Blocking ICAM-1 and CD11a inhibits monocyte-EC adhesion. HUVECs and monocytes were treated with 4 mM AA or 8 mM BHB for 24 h. Following treatment, HUVEC monolayers were treated with 5 mg/ml mouse IgG antibody or 5 mg/ml ICAM-1 blocking antibody for 30

    Article Snippet: The following primary antibodies used in this study were: rabbit polyclonal anti-CD11a, for detection of LFA-1, and mouse monoclonal anti-β-actin (Abcam, Cambridge, MA); mouse monoclonal anti-E-selectin, -ICAM-1, and -vascular cell adhesion molecule-1 (VCAM-1) (Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal anti-ICAM-1 for blocking (US Biological, Swampscott, MA); mouse monoclonal anti-CD11a for blocking (Abcam), mouse monoclonal anti-CD11+CD18 (mAb24) for activation epitope, and mouse monoclonal IgG1 (Abcam).

    Techniques: Blocking Assay

    ECAM protein expression in HUVECs. A : total protein expression of intercellular adhesion molecule-1 (ICAM-1). HUVECs were plated in 100-mm dishes and treated with AA or BHB (0–8 mM). ICAM-1 was detected via Western blotting as described in materials

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells

    doi: 10.1152/ajpendo.00038.2011

    Figure Lengend Snippet: ECAM protein expression in HUVECs. A : total protein expression of intercellular adhesion molecule-1 (ICAM-1). HUVECs were plated in 100-mm dishes and treated with AA or BHB (0–8 mM). ICAM-1 was detected via Western blotting as described in materials

    Article Snippet: The following primary antibodies used in this study were: rabbit polyclonal anti-CD11a, for detection of LFA-1, and mouse monoclonal anti-β-actin (Abcam, Cambridge, MA); mouse monoclonal anti-E-selectin, -ICAM-1, and -vascular cell adhesion molecule-1 (VCAM-1) (Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal anti-ICAM-1 for blocking (US Biological, Swampscott, MA); mouse monoclonal anti-CD11a for blocking (Abcam), mouse monoclonal anti-CD11+CD18 (mAb24) for activation epitope, and mouse monoclonal IgG1 (Abcam).

    Techniques: Expressing, Western Blot