icam1  (Sino Biological)


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    Name:
    ICAM 1 Protein Rhesus Recombinant
    Description:
    A DNA sequence encoding the ICAM1 NP 001040600 1 Met1 Glu480 was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    90320-C08H
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological icam1
    Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, <t>ICAM1,</t> VCAM1 in HBMECs ( A ) or HUVEC ( C ) after
    A DNA sequence encoding the ICAM1 NP 001040600 1 Met1 Glu480 was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/icam1/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    icam1 - by Bioz Stars, 2021-07
    94/100 stars

    Images

    1) Product Images from "Brain endothelial dysfunction in cerebral adrenoleukodystrophy"

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy

    Journal: Brain

    doi: 10.1093/brain/awv250

    Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after
    Figure Legend Snippet: Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after

    Techniques Used: Expressing, Western Blot

    2) Product Images from "Brain endothelial dysfunction in cerebral adrenoleukodystrophy"

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy

    Journal: Brain

    doi: 10.1093/brain/awv250

    Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after
    Figure Legend Snippet: Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after

    Techniques Used: Expressing, Western Blot

    3) Product Images from "Brain endothelial dysfunction in cerebral adrenoleukodystrophy"

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy

    Journal: Brain

    doi: 10.1093/brain/awv250

    Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after
    Figure Legend Snippet: Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after

    Techniques Used: Expressing, Western Blot

    4) Product Images from "Brain endothelial dysfunction in cerebral adrenoleukodystrophy"

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy

    Journal: Brain

    doi: 10.1093/brain/awv250

    Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after
    Figure Legend Snippet: Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after

    Techniques Used: Expressing, Western Blot

    Related Articles

    Staining:

    Article Title: Plasmodium falciparum erythrocyte membrane protein 1 variants induce cell swelling and disrupt the blood–brain barrier in cerebral malaria
    Article Snippet: .. ICAM-1 was visualized by staining with anti–ICAM-1 (10 µg/ml; rabbit polyclonal; SinoBiological) for 1 h at room temperature. .. Coverslips were then washed and incubated for 45 min with anti-rabbit IgG Alexa 488 (1:500; 100 µl 5% FBS/PBS plus 0.0025% Tween 20) in the dark.

    Produced:

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy
    Article Snippet: .. However, high concentrations of VLCFAs produced only mild elevations of ICAM1 and yielded no changes in CLDN5 in HBMECs ( B). .. In summary, ABCD1 -silenced HBMECs altered their protein expression of ICAM1, VCAM1, CLDN5 and ZO1 > 24 h before VLCFA increases, indicating an independent effect of the ABCD1 gene on adhesion molecules and tight junction proteins.

    Expressing:

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy
    Article Snippet: To assess the role of c-MYC in ABCD1-deficient endothelial cells, we downregulated c-MYC expression in HBMECs using siRNA. .. Interestingly, MYC silencing changed the expression of CLDN5 and ICAM1 in a similar manner as ABCD1 silencing without decreasing the levels of ABCD1 protein itself ( C, P < 0.001). .. In parallel with protein marker changes, silencing of MYC also increased THP-1 adhesion to HBMEC monolayers ( D, P < 0.001) at both basal level and in TNFα-treated conditions.

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy
    Article Snippet: .. Of note, ABCD1 silencing led to significant changes of ICAM1 and CLDN5 protein expression—around 80% reduction in CLDN5 ( P < 0.001) and 70% increase in ICAM1 ( P < 0.05) ( A and B), along with moderate reduction of ZO1 expression in HBMECs. .. Upon further analysis of mRNA levels, the dramatic reduction of CLDN5 mRNA expression indicated transcriptional inhibition by ABCD1 deficiency, while a lack of change in ICAM1 gene expression suggested likely post-transcriptional upregulation of ICAM1 protein ( C, P < 0.01).

    Recombinant:

    Article Title: The receptors CD96 and CD226 oppose each other in the regulation of natural killer cell functions.
    Article Snippet: CD96, CD226 (DNAM-1) and TIGIT belong to an emerging family of receptors that interact with nectin and nectin-like proteins. .. CD96, CD226 (DNAM-1) and TIGIT belong to an emerging family of receptors that interact with nectin and nectin-like proteins. .. CD96, CD226 (DNAM-1) and TIGIT belong to an emerging family of receptors that interact with nectin and nectin-like proteins.

    Immunostaining:

    Article Title: Acute administration of catalase targeted to ICAM-1 attenuates neuropathology in experimental traumatic brain injury
    Article Snippet: ImageJ software (1.48 v http://rsb.info.nih.gov/ij/ ; Bethesda, MD) was used to threshold for fluorescence intensity and to convert to binary images. .. For analysis of chromogen immunostaining of ICAM-1, GFAP, Iba1, NeuN, and 3-NT, ImageJ analysis was performed by batch processing with an automated cell-counting macro. .. The macro was configured to perform particle counting on high resolution images (5/sample, 3/group, taken at 20X objective magnification) on immunolabeled cells.

    Cell Counting:

    Article Title: Acute administration of catalase targeted to ICAM-1 attenuates neuropathology in experimental traumatic brain injury
    Article Snippet: ImageJ software (1.48 v http://rsb.info.nih.gov/ij/ ; Bethesda, MD) was used to threshold for fluorescence intensity and to convert to binary images. .. For analysis of chromogen immunostaining of ICAM-1, GFAP, Iba1, NeuN, and 3-NT, ImageJ analysis was performed by batch processing with an automated cell-counting macro. .. The macro was configured to perform particle counting on high resolution images (5/sample, 3/group, taken at 20X objective magnification) on immunolabeled cells.

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  • 94
    Sino Biological icam1
    Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, <t>ICAM1,</t> VCAM1 in HBMECs ( A ) or HUVEC ( C ) after
    Icam1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icam1/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    icam1 - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

    86
    Sino Biological mouse icam 1
    Labeling monocytes with IRDye 800CW: dye stability and the adhesion o mICAM-1. (A) Labeling efficiency of monocytes using one-step IRDye800CW-conjugated anti-CD14 mAb. (B) Two-step labeling using IRDye800-conjugated secondary antibody to the following primary mAbs: a, anti-CD14; b, anti-CD16; c, anti-CD64; d, anti-CD14, anti-CD16 and anti-CD64; e, isotype 1 (mouse IgG2a); f, isotype 2 (mouse IgG1); g, isotypes 1 and 2; h, secondary Ab only; i, PBS (negative control). (C) Direct conjugation using the indicated different concentrations of the IRDye 800CW NHS ester. (D) Monocyte viability. (E) Labeling stability over time. (F) Monocyte adhesion to <t>ICAM-1.</t> Data are presented as means ± SDs for three or more independent experiments. One-way ANOVA followed by the Bonferroni correction test was used for the analysis in (C), one-way ANOVA followed by the Kruskal-Wallis test was used for the analysis in (D), and a t-test was used for the analysis in (F). n.s. no significant difference; *P
    Mouse Icam 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse icam 1/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse icam 1 - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    95
    Sino Biological rabbit anti icam 1 antibodies
    Inhibition of RV89 binding to <t>ICAM-1</t> by antibodies specific for each of the four viral capsid proteins (VP). Binding of RV89 to ICAM-1 after pre-incubation of virus with antibodies against VP1, VP2, VP3, and VP4 compared to pre-incubation with buffer containing BSA only reported as O.D. (y-axes). The inhibitions of RV89 binding by anti-VP1 and anti-VP2 antibodies are indicated by red arrows. Omission of RV89 served as a negative control. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments were performed three times.
    Rabbit Anti Icam 1 Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti icam 1 antibodies/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti icam 1 antibodies - by Bioz Stars, 2021-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after

    Journal: Brain

    Article Title: Brain endothelial dysfunction in cerebral adrenoleukodystrophy

    doi: 10.1093/brain/awv250

    Figure Lengend Snippet: Comparison of tight-junction protein and adhesion molecule expression after ABCD1 silencing between HUVECs and HBMECs at both basal and TNFα-stimulated condition. Western blot depicting ABCD1, CLDN5, ICAM1, VCAM1 in HBMECs ( A ) or HUVEC ( C ) after

    Article Snippet: However, high concentrations of VLCFAs produced only mild elevations of ICAM1 and yielded no changes in CLDN5 in HBMECs ( B).

    Techniques: Expressing, Western Blot

    Labeling monocytes with IRDye 800CW: dye stability and the adhesion o mICAM-1. (A) Labeling efficiency of monocytes using one-step IRDye800CW-conjugated anti-CD14 mAb. (B) Two-step labeling using IRDye800-conjugated secondary antibody to the following primary mAbs: a, anti-CD14; b, anti-CD16; c, anti-CD64; d, anti-CD14, anti-CD16 and anti-CD64; e, isotype 1 (mouse IgG2a); f, isotype 2 (mouse IgG1); g, isotypes 1 and 2; h, secondary Ab only; i, PBS (negative control). (C) Direct conjugation using the indicated different concentrations of the IRDye 800CW NHS ester. (D) Monocyte viability. (E) Labeling stability over time. (F) Monocyte adhesion to ICAM-1. Data are presented as means ± SDs for three or more independent experiments. One-way ANOVA followed by the Bonferroni correction test was used for the analysis in (C), one-way ANOVA followed by the Kruskal-Wallis test was used for the analysis in (D), and a t-test was used for the analysis in (F). n.s. no significant difference; *P

    Journal: Theranostics

    Article Title: Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery

    doi: 10.7150/thno.29223

    Figure Lengend Snippet: Labeling monocytes with IRDye 800CW: dye stability and the adhesion o mICAM-1. (A) Labeling efficiency of monocytes using one-step IRDye800CW-conjugated anti-CD14 mAb. (B) Two-step labeling using IRDye800-conjugated secondary antibody to the following primary mAbs: a, anti-CD14; b, anti-CD16; c, anti-CD64; d, anti-CD14, anti-CD16 and anti-CD64; e, isotype 1 (mouse IgG2a); f, isotype 2 (mouse IgG1); g, isotypes 1 and 2; h, secondary Ab only; i, PBS (negative control). (C) Direct conjugation using the indicated different concentrations of the IRDye 800CW NHS ester. (D) Monocyte viability. (E) Labeling stability over time. (F) Monocyte adhesion to ICAM-1. Data are presented as means ± SDs for three or more independent experiments. One-way ANOVA followed by the Bonferroni correction test was used for the analysis in (C), one-way ANOVA followed by the Kruskal-Wallis test was used for the analysis in (D), and a t-test was used for the analysis in (F). n.s. no significant difference; *P

    Article Snippet: Using a microfluidic setup, we found that monocytes effectively adhered to mouse ICAM-1 but showed almost no adhesion to a non-coated (PBS treated) surface (Figure F).

    Techniques: Labeling, Negative Control, Conjugation Assay

    Inhibition of RV89 binding to ICAM-1 by antibodies specific for each of the four viral capsid proteins (VP). Binding of RV89 to ICAM-1 after pre-incubation of virus with antibodies against VP1, VP2, VP3, and VP4 compared to pre-incubation with buffer containing BSA only reported as O.D. (y-axes). The inhibitions of RV89 binding by anti-VP1 and anti-VP2 antibodies are indicated by red arrows. Omission of RV89 served as a negative control. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments were performed three times.

    Journal: Vaccines

    Article Title: ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions

    doi: 10.3390/vaccines8020315

    Figure Lengend Snippet: Inhibition of RV89 binding to ICAM-1 by antibodies specific for each of the four viral capsid proteins (VP). Binding of RV89 to ICAM-1 after pre-incubation of virus with antibodies against VP1, VP2, VP3, and VP4 compared to pre-incubation with buffer containing BSA only reported as O.D. (y-axes). The inhibitions of RV89 binding by anti-VP1 and anti-VP2 antibodies are indicated by red arrows. Omission of RV89 served as a negative control. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments were performed three times.

    Article Snippet: On the following day, cells were incubated with 1:100 to 1:100,000 diluted rabbit anti-ICAM-1 antibodies or normal rabbit antibodies that had been heat-inactivated for 30 min at 56 °C, for 3 h at 37 °C in triplicate cultures.

    Techniques: Inhibition, Binding Assay, Incubation, Negative Control

    Inhibition of RV89 infection by anti-ICAM-1 antibodies determined by cell culture-based virus inhibition assay. ( a ) Crystal violet-stained viable cells incubated only with anti-ICAM-1 or non-specific antibodies (top), different dilutions of antibodies (1:100–1:100,000); anti-ICAM-1 and non-specific antibodies separated by red vertical line) in the presence of virus (100 TCID 50 ), 1:100 diluted antibodies without virus, cell incubated only with virus (100 TCID 50 ), or only with medium (bottom). ( b ) Mean O.D. values corresponding to viable cells in triplicate wells ±SDs ( y -axis) of the above experiment ( x -axis: conditions applied). Neutralization tests were repeated two times.

    Journal: Vaccines

    Article Title: ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions

    doi: 10.3390/vaccines8020315

    Figure Lengend Snippet: Inhibition of RV89 infection by anti-ICAM-1 antibodies determined by cell culture-based virus inhibition assay. ( a ) Crystal violet-stained viable cells incubated only with anti-ICAM-1 or non-specific antibodies (top), different dilutions of antibodies (1:100–1:100,000); anti-ICAM-1 and non-specific antibodies separated by red vertical line) in the presence of virus (100 TCID 50 ), 1:100 diluted antibodies without virus, cell incubated only with virus (100 TCID 50 ), or only with medium (bottom). ( b ) Mean O.D. values corresponding to viable cells in triplicate wells ±SDs ( y -axis) of the above experiment ( x -axis: conditions applied). Neutralization tests were repeated two times.

    Article Snippet: On the following day, cells were incubated with 1:100 to 1:100,000 diluted rabbit anti-ICAM-1 antibodies or normal rabbit antibodies that had been heat-inactivated for 30 min at 56 °C, for 3 h at 37 °C in triplicate cultures.

    Techniques: Inhibition, Infection, Cell Culture, Staining, Incubation, Neutralization

    Binding of major group and minor group RVs to ICAM-1and LDLR. Shown are O.D. values (y-axes) corresponding to receptor (ICAM-1 or LDLR)-bound ( a ) major or ( b ) minor group RVs (x-axes) as determined by ELISA. Tested major group RV-A or RV-B species ( a ) and minor group RV-A species ( b ) are indicated on the x-axes. The analyses were performed with virus (50 µg/mL RV) and, for control purposes, without virus (−RV), without virus and detection antibodies (−RV, −anti-RV) or with HSA- instead of receptor-coated plates (HSA coated). The results are means of duplicate determinations with a variation of less than 5%. RV–receptor binding experiments were repeated two times.

    Journal: Vaccines

    Article Title: ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions

    doi: 10.3390/vaccines8020315

    Figure Lengend Snippet: Binding of major group and minor group RVs to ICAM-1and LDLR. Shown are O.D. values (y-axes) corresponding to receptor (ICAM-1 or LDLR)-bound ( a ) major or ( b ) minor group RVs (x-axes) as determined by ELISA. Tested major group RV-A or RV-B species ( a ) and minor group RV-A species ( b ) are indicated on the x-axes. The analyses were performed with virus (50 µg/mL RV) and, for control purposes, without virus (−RV), without virus and detection antibodies (−RV, −anti-RV) or with HSA- instead of receptor-coated plates (HSA coated). The results are means of duplicate determinations with a variation of less than 5%. RV–receptor binding experiments were repeated two times.

    Article Snippet: On the following day, cells were incubated with 1:100 to 1:100,000 diluted rabbit anti-ICAM-1 antibodies or normal rabbit antibodies that had been heat-inactivated for 30 min at 56 °C, for 3 h at 37 °C in triplicate cultures.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

    Inhibition of major group RV89 and minor group RV2 binding to plate-bound receptors by receptor-specific antibodies. Binding of RV89 (upper part) ( a ) and RV2 (lower part) ( b ) to plate-bound ICAM-1 (left panels) or plate-bound LDLR (right panels) reported as mean O.D. values (y-axes) in the presence (+) or absence (−) of virus, commercial anti-ICAM-1 antibodies, commercial anti-LDLR antibodies, anti-RV detection antibodies, and secondary detection antibodies (anti-GP) (see below x-axes). The percentage inhibitions of RV89 binding and RV2 binding obtained with commercial anti-receptor antibodies compared to without anti-receptor antibodies are indicated in red. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments were performed once.

    Journal: Vaccines

    Article Title: ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions

    doi: 10.3390/vaccines8020315

    Figure Lengend Snippet: Inhibition of major group RV89 and minor group RV2 binding to plate-bound receptors by receptor-specific antibodies. Binding of RV89 (upper part) ( a ) and RV2 (lower part) ( b ) to plate-bound ICAM-1 (left panels) or plate-bound LDLR (right panels) reported as mean O.D. values (y-axes) in the presence (+) or absence (−) of virus, commercial anti-ICAM-1 antibodies, commercial anti-LDLR antibodies, anti-RV detection antibodies, and secondary detection antibodies (anti-GP) (see below x-axes). The percentage inhibitions of RV89 binding and RV2 binding obtained with commercial anti-receptor antibodies compared to without anti-receptor antibodies are indicated in red. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments were performed once.

    Article Snippet: On the following day, cells were incubated with 1:100 to 1:100,000 diluted rabbit anti-ICAM-1 antibodies or normal rabbit antibodies that had been heat-inactivated for 30 min at 56 °C, for 3 h at 37 °C in triplicate cultures.

    Techniques: Inhibition, Binding Assay

    Inhibition of RV89 and RV14 binding to plate-bound receptors by receptor-specific antibodies. Binding of RV89 (upper part) ( a ) and RV14 (lower part) ( b ) to plate-bound ICAM-1 (left panels) or plate-bound LDLR (right panels) is reported as mean O.D. values (y-axes) in the presence (+) or absence (−) of virus, anti-ICAM-1 antibodies, non-specific antibodies, anti-RV detection antibodies, and secondary detection antibodies (anti-GP) (below x-axes). The percentage inhibitions of RV89 binding and RV14 binding obtained with anti-ICAM-1 antibodies versus non-specific antibodies are indicated in red. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments were repeated three times.

    Journal: Vaccines

    Article Title: ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions

    doi: 10.3390/vaccines8020315

    Figure Lengend Snippet: Inhibition of RV89 and RV14 binding to plate-bound receptors by receptor-specific antibodies. Binding of RV89 (upper part) ( a ) and RV14 (lower part) ( b ) to plate-bound ICAM-1 (left panels) or plate-bound LDLR (right panels) is reported as mean O.D. values (y-axes) in the presence (+) or absence (−) of virus, anti-ICAM-1 antibodies, non-specific antibodies, anti-RV detection antibodies, and secondary detection antibodies (anti-GP) (below x-axes). The percentage inhibitions of RV89 binding and RV14 binding obtained with anti-ICAM-1 antibodies versus non-specific antibodies are indicated in red. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments were repeated three times.

    Article Snippet: On the following day, cells were incubated with 1:100 to 1:100,000 diluted rabbit anti-ICAM-1 antibodies or normal rabbit antibodies that had been heat-inactivated for 30 min at 56 °C, for 3 h at 37 °C in triplicate cultures.

    Techniques: Inhibition, Binding Assay

    Characterization of recombinant human ICAM-1 and LDLR. ( a ) Coomassie-blue stained SDS-PAGE with recombinant human ICAM-1 (lane 1) and LDLR (lane 2). A molecular-weight marker (kDa) is indicated on the left. ( b ) Mean residue ellipticities (θ, y -axis) of ICAM-1 (red) and LDLR (blue) recorded at different wavelengths (nm, x -axis) by circular dichroism (CD) spectroscopy. Detection of ( c ) ICAM-1 or ( d ) LDLR with serial dilutions of anti-ICAM-1 antibodies (c = 1 µg/mL; left), anti-LDLR antibodies (c = 0.2 µg/mL; right) or bovine serum albumin (BSA)-containing buffer alone (BSA) by ELISA. Shown are means of optical density (O.D.) values corresponding to bound antibodies (y-axes) measured at different time points (x-axes). The variations of individual duplicate results of ELISAs were less than 5%. The antibody titration in ( c ) and ( d ) was done once.

    Journal: Vaccines

    Article Title: ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions

    doi: 10.3390/vaccines8020315

    Figure Lengend Snippet: Characterization of recombinant human ICAM-1 and LDLR. ( a ) Coomassie-blue stained SDS-PAGE with recombinant human ICAM-1 (lane 1) and LDLR (lane 2). A molecular-weight marker (kDa) is indicated on the left. ( b ) Mean residue ellipticities (θ, y -axis) of ICAM-1 (red) and LDLR (blue) recorded at different wavelengths (nm, x -axis) by circular dichroism (CD) spectroscopy. Detection of ( c ) ICAM-1 or ( d ) LDLR with serial dilutions of anti-ICAM-1 antibodies (c = 1 µg/mL; left), anti-LDLR antibodies (c = 0.2 µg/mL; right) or bovine serum albumin (BSA)-containing buffer alone (BSA) by ELISA. Shown are means of optical density (O.D.) values corresponding to bound antibodies (y-axes) measured at different time points (x-axes). The variations of individual duplicate results of ELISAs were less than 5%. The antibody titration in ( c ) and ( d ) was done once.

    Article Snippet: On the following day, cells were incubated with 1:100 to 1:100,000 diluted rabbit anti-ICAM-1 antibodies or normal rabbit antibodies that had been heat-inactivated for 30 min at 56 °C, for 3 h at 37 °C in triplicate cultures.

    Techniques: Recombinant, Staining, SDS Page, Molecular Weight, Marker, Spectroscopy, Enzyme-linked Immunosorbent Assay, Titration

    Auto-inhibition of the binding of major group RV89 and minor group RV2 to their receptors. Binding of RV89 to ICAM-1 ( left ) and of RV2 to LDLR ( right ) without (first column) or with (second column) pre-incubation with the receptor plus controls in which virus and receptor (third column) or virus, receptor and detection antibodies (fourth column) were omitted, reported as mean optical density (i.e., O.D.) values (y-axes). The percentage inhibitions of RV89 and RV2 binding produced by pre-incubation with receptors are indicated in red. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments with soluble receptors were performed two times.

    Journal: Vaccines

    Article Title: ELISA-Based Assay for Studying Major and Minor Group Rhinovirus–Receptor Interactions

    doi: 10.3390/vaccines8020315

    Figure Lengend Snippet: Auto-inhibition of the binding of major group RV89 and minor group RV2 to their receptors. Binding of RV89 to ICAM-1 ( left ) and of RV2 to LDLR ( right ) without (first column) or with (second column) pre-incubation with the receptor plus controls in which virus and receptor (third column) or virus, receptor and detection antibodies (fourth column) were omitted, reported as mean optical density (i.e., O.D.) values (y-axes). The percentage inhibitions of RV89 and RV2 binding produced by pre-incubation with receptors are indicated in red. The results are means of duplicate determinations with a variation of less than 10%. Inhibition experiments with soluble receptors were performed two times.

    Article Snippet: On the following day, cells were incubated with 1:100 to 1:100,000 diluted rabbit anti-ICAM-1 antibodies or normal rabbit antibodies that had been heat-inactivated for 30 min at 56 °C, for 3 h at 37 °C in triplicate cultures.

    Techniques: Inhibition, Binding Assay, Incubation, Produced