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Pharmingen icam 1
Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( <t>ICAM-1</t> ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
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1) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

2) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

3) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

4) Product Images from "Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages"

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

Journal: Journal of Virology

doi: 10.1128/JVI.77.18.10125-10130.2003

Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.
Figure Legend Snippet: Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.

Techniques Used: Infection, FACS, Expressing

MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.
Figure Legend Snippet: MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.

Techniques Used: Blocking Assay, Expressing, FACS, Infection

MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
Figure Legend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Techniques Used: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection

5) Product Images from "Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages"

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

Journal: Journal of Virology

doi: 10.1128/JVI.77.18.10125-10130.2003

Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.
Figure Legend Snippet: Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.

Techniques Used: Infection, FACS, Expressing

MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.
Figure Legend Snippet: MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.

Techniques Used: Blocking Assay, Expressing, FACS, Infection

MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
Figure Legend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Techniques Used: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection

6) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

7) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

8) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

9) Product Images from "Systemic treatment with anti-CD40 antibody stimulates Langerhans cell migration from the skin"

Article Title: Systemic treatment with anti-CD40 antibody stimulates Langerhans cell migration from the skin

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2002.01909.x

The effects of anti-CD40 antibodies on the phenotype of epidermal Langerhans cells. (a) BALB/c mice injected with either anti CD40 antibody (3/23) or control antibody (MAC 193) were killed after 3 days. Epidermal cell suspensions were made, the cells stained for MHC Class II, CD86 and ICAM-1 and analysed by flow cytometry. The expression of MHC Class II, CD86 and ICAM-1 on fresh Langerhans cells isolated from ear skin of normal mice were compared. The level of expression of these markers of maturity is increased in the CD40 treated LC. (b) In vitro matured epidermal Langerhans cells isolated from 48h skin explant organ cultures and migrated LC were used to compare the expression of CD86. The expression of CD86 is increased in the CD40 treated LC but not to the extent of the epidermal LC which have a bimodal expression of CD86 or the migrated LC derived from explants.
Figure Legend Snippet: The effects of anti-CD40 antibodies on the phenotype of epidermal Langerhans cells. (a) BALB/c mice injected with either anti CD40 antibody (3/23) or control antibody (MAC 193) were killed after 3 days. Epidermal cell suspensions were made, the cells stained for MHC Class II, CD86 and ICAM-1 and analysed by flow cytometry. The expression of MHC Class II, CD86 and ICAM-1 on fresh Langerhans cells isolated from ear skin of normal mice were compared. The level of expression of these markers of maturity is increased in the CD40 treated LC. (b) In vitro matured epidermal Langerhans cells isolated from 48h skin explant organ cultures and migrated LC were used to compare the expression of CD86. The expression of CD86 is increased in the CD40 treated LC but not to the extent of the epidermal LC which have a bimodal expression of CD86 or the migrated LC derived from explants.

Techniques Used: Mouse Assay, Injection, Staining, Flow Cytometry, Cytometry, Expressing, Isolation, In Vitro, Derivative Assay

10) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

11) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

12) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

13) Product Images from "Transcriptional Activation of mRNA of Intercellular Adhesion Molecule 1 and Induction of Its Cell Surface Expression in Normal Human Gingival Fibroblasts by Mycoplasma salivarium and Mycoplasma fermentans"

Article Title: Transcriptional Activation of mRNA of Intercellular Adhesion Molecule 1 and Induction of Its Cell Surface Expression in Normal Human Gingival Fibroblasts by Mycoplasma salivarium and Mycoplasma fermentans

Journal: Infection and Immunity

doi:

Analysis of expression of mRNAs of β-actin, ICAM-1, and VCAM-1 in Gin-1 cells stimulated with CM of M. salivarium (salm) or M. fermentans (ferm). The confluent monolayers of Gin-1 cells in 3.5-cm-diameter culture dishes were incubated at 37°C for 6 h in the absence (None) or the presence of CM of M. salivarium (salm) or M. fermentans (ferm) at a protein concentration of 40 μg/ml. The RNAs were prepared from Gin-1 cells and analyzed by RT-PCR.
Figure Legend Snippet: Analysis of expression of mRNAs of β-actin, ICAM-1, and VCAM-1 in Gin-1 cells stimulated with CM of M. salivarium (salm) or M. fermentans (ferm). The confluent monolayers of Gin-1 cells in 3.5-cm-diameter culture dishes were incubated at 37°C for 6 h in the absence (None) or the presence of CM of M. salivarium (salm) or M. fermentans (ferm) at a protein concentration of 40 μg/ml. The RNAs were prepared from Gin-1 cells and analyzed by RT-PCR.

Techniques Used: Expressing, Incubation, Protein Concentration, Reverse Transcription Polymerase Chain Reaction

Effect of polymyxin B on cell surface expression of ICAM-1 on Gin-1 cells stimulated with Lpsal or E. coli LPS. Gin-1 cells were grown in DME medium until they reached confluency. The confluent monolayers of Gin-1 cells were preincubated for 37°C for 1 h with polymyxin B (5,000 or 10,000 U/ml) in the presence of Lpsal (50 μg of protein/ml [■]) or E. coli LPS (1 μg/ml [□]) and then examined for cell surface expression of ICAM-1 on Gin-1 cells by Cell-ELISA.
Figure Legend Snippet: Effect of polymyxin B on cell surface expression of ICAM-1 on Gin-1 cells stimulated with Lpsal or E. coli LPS. Gin-1 cells were grown in DME medium until they reached confluency. The confluent monolayers of Gin-1 cells were preincubated for 37°C for 1 h with polymyxin B (5,000 or 10,000 U/ml) in the presence of Lpsal (50 μg of protein/ml [■]) or E. coli LPS (1 μg/ml [□]) and then examined for cell surface expression of ICAM-1 on Gin-1 cells by Cell-ELISA.

Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

Analysis of expression of mRNAs of β-actin and ICAM-1 in Gin-F and PDL-F as well as Gin-1 cells stimulated with Lpsal. The confluent monolayers of Gin-F and PDL-F as well as Gin-1 cells in 3.5-cm-diameter culture dishes were incubated at 37°C for 6 h in the absence (None) or the presence of Lpsal at a protein concentration of 60 μg/ml. The expression of mRNAs of β-actin or ICAM-1 was analyzed by RT-PCR.
Figure Legend Snippet: Analysis of expression of mRNAs of β-actin and ICAM-1 in Gin-F and PDL-F as well as Gin-1 cells stimulated with Lpsal. The confluent monolayers of Gin-F and PDL-F as well as Gin-1 cells in 3.5-cm-diameter culture dishes were incubated at 37°C for 6 h in the absence (None) or the presence of Lpsal at a protein concentration of 60 μg/ml. The expression of mRNAs of β-actin or ICAM-1 was analyzed by RT-PCR.

Techniques Used: Expressing, Incubation, Protein Concentration, Reverse Transcription Polymerase Chain Reaction

14) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

15) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

16) Product Images from "Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages"

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

Journal: Journal of Virology

doi: 10.1128/JVI.77.18.10125-10130.2003

Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.
Figure Legend Snippet: Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.

Techniques Used: Infection, FACS, Expressing

MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.
Figure Legend Snippet: MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.

Techniques Used: Blocking Assay, Expressing, FACS, Infection

MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
Figure Legend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Techniques Used: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection

17) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

18) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

19) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

20) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

21) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

22) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

23) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

24) Product Images from "The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node"

Article Title: The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node

Journal: European journal of immunology

doi: 10.1002/1521-4141(2001010)31:10 < 3085::AID-IMMU3085 > 3.0.CO;2-B

Immunohistochemical staining of epidermal Langerhans cells. The epidermis of naive or hapten-treated ICAM-1-deficient and wild-type mice was stained with FITC-labeled anti-Ia b antibody. The photos indicate MHC class II + Langerhans cells in the naïve and hapten-treated epidermis at 4 or 24 h following hapten application. The bar graphs indicate the average number of MHC class II + Langerhans cells per mm 2 in each experimental group with six mice. The difference between wild-type C57BL/6 and ICAM-1-deficient mice is not statistically significant at the indicated times.
Figure Legend Snippet: Immunohistochemical staining of epidermal Langerhans cells. The epidermis of naive or hapten-treated ICAM-1-deficient and wild-type mice was stained with FITC-labeled anti-Ia b antibody. The photos indicate MHC class II + Langerhans cells in the naïve and hapten-treated epidermis at 4 or 24 h following hapten application. The bar graphs indicate the average number of MHC class II + Langerhans cells per mm 2 in each experimental group with six mice. The difference between wild-type C57BL/6 and ICAM-1-deficient mice is not statistically significant at the indicated times.

Techniques Used: Immunohistochemistry, Staining, Mouse Assay, Labeling, IA

The effect of ICAM-1 deficiency on DC migration out of the skin. (A) Immunohistochemical staining on skin cross-sections with anti-Ia b antibody. Mice were sensitized with hapten and the skin was taken 4 h later to prepare frozen cross-sections. The photos show representative results of four mice in each group. (B) DC migration out of skin explants in cultures. Mice were sensitized on ears and hapten-treated skin samples were taken 2 h later and placed in cultures. The migratory cells were harvested and counted 3 days after the culture. The result shows the total cell number from five mice (ten ears) of each group.
Figure Legend Snippet: The effect of ICAM-1 deficiency on DC migration out of the skin. (A) Immunohistochemical staining on skin cross-sections with anti-Ia b antibody. Mice were sensitized with hapten and the skin was taken 4 h later to prepare frozen cross-sections. The photos show representative results of four mice in each group. (B) DC migration out of skin explants in cultures. Mice were sensitized on ears and hapten-treated skin samples were taken 2 h later and placed in cultures. The migratory cells were harvested and counted 3 days after the culture. The result shows the total cell number from five mice (ten ears) of each group.

Techniques Used: Migration, Immunohistochemistry, Staining, IA, Mouse Assay

In vivo migration assay for determination of DC migration into the regional lymph node. Bone marrow-derived dendritic cells were used for the in vivo migration assay. (A) The phenotype of bone marrow-derived DC from C57BL/6 and ICAM-1 knockout mice. (B) The migration index of 51 Cr-labeled DC in the draining popliteal lymph node at 18–20 h after injection. The migration index is calculated as described in Sect. 4 and the bar graph shows the data of eight mice in each group. The difference in the migration index between C57BL/6 and ICAM-1-deficient recipient mice is significant ( p
Figure Legend Snippet: In vivo migration assay for determination of DC migration into the regional lymph node. Bone marrow-derived dendritic cells were used for the in vivo migration assay. (A) The phenotype of bone marrow-derived DC from C57BL/6 and ICAM-1 knockout mice. (B) The migration index of 51 Cr-labeled DC in the draining popliteal lymph node at 18–20 h after injection. The migration index is calculated as described in Sect. 4 and the bar graph shows the data of eight mice in each group. The difference in the migration index between C57BL/6 and ICAM-1-deficient recipient mice is significant ( p

Techniques Used: In Vivo, Migration, Derivative Assay, Knock-Out, Mouse Assay, Labeling, Injection

25) Product Images from "Activation of NF-?B by the Human Herpesvirus 8 Chemokine Receptor ORF74: Evidence for a Paracrine Model of Kaposi's Sarcoma Pathogenesis"

Article Title: Activation of NF-?B by the Human Herpesvirus 8 Chemokine Receptor ORF74: Evidence for a Paracrine Model of Kaposi's Sarcoma Pathogenesis

Journal: Journal of Virology

doi: 10.1128/JVI.75.18.8660-8673.2001

Induction of adhesion molecule expression by ORF74. KSIMM cells were transfected with pSG5-ORF74 or pSG5 alone. Cells were detached at 20 h posttransfection with 1 mM EDTA and counted. Aliquots (0.5 × 10 6 ) of transfected cells were stained with the indicated antibodies. Cells treated with TNF-α (5 ng/ml) were included as a positive control for the induction of adhesion marker expression (data not shown). Samples were analyzed using a Becton Dickinson FACSCalibur flow cytometer. PE-conjugated antibodies for VCAM-1, ICAM-1, E-selectin, and αVβ3 integrin were obtained from PharMingen. Appropriate isotype-matched controls were used for each antibody as a control for nonspecific antibody binding. Cell Quest Analysis (Becton Dickinson version 3.1f) software was used to analyze the raw fluorescence-activated cell-sorting data and obtain quantitative values. (A) Analysis of expression of cell surface ICAM-1. (B) Analysis of expression of cell surface VCAM-1. (C) Analysis of expression of cell surface E-selectin. (D) Analysis of expression of cell surface αVβ3. The solid line represents the isotype control, the dotted line represents pSG5-transfected cells, and the dashed line represents ORF74-transfected cells. The transfection efficiency was ca. 35%.
Figure Legend Snippet: Induction of adhesion molecule expression by ORF74. KSIMM cells were transfected with pSG5-ORF74 or pSG5 alone. Cells were detached at 20 h posttransfection with 1 mM EDTA and counted. Aliquots (0.5 × 10 6 ) of transfected cells were stained with the indicated antibodies. Cells treated with TNF-α (5 ng/ml) were included as a positive control for the induction of adhesion marker expression (data not shown). Samples were analyzed using a Becton Dickinson FACSCalibur flow cytometer. PE-conjugated antibodies for VCAM-1, ICAM-1, E-selectin, and αVβ3 integrin were obtained from PharMingen. Appropriate isotype-matched controls were used for each antibody as a control for nonspecific antibody binding. Cell Quest Analysis (Becton Dickinson version 3.1f) software was used to analyze the raw fluorescence-activated cell-sorting data and obtain quantitative values. (A) Analysis of expression of cell surface ICAM-1. (B) Analysis of expression of cell surface VCAM-1. (C) Analysis of expression of cell surface E-selectin. (D) Analysis of expression of cell surface αVβ3. The solid line represents the isotype control, the dotted line represents pSG5-transfected cells, and the dashed line represents ORF74-transfected cells. The transfection efficiency was ca. 35%.

Techniques Used: Expressing, Transfection, Staining, Positive Control, Marker, Flow Cytometry, Cytometry, Binding Assay, Software, Fluorescence, FACS

26) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

27) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

28) Product Images from "Plasma fibronectin deficiency impedes atherosclerosis progression and fibrous cap formation"

Article Title: Plasma fibronectin deficiency impedes atherosclerosis progression and fibrous cap formation

Journal: EMBO Molecular Medicine

doi: 10.1002/emmm.201200237

Reduced NF-κB and PAK activation and macrophage recruitment into atheroprone areas of ApoE −/− FN MxCre mice Immunostaining of phosho-NFκB/p65, phospho-PAK, phospho-JNK and ICAM-1 in the lesser curvature of the aortic arch from ApoE −/− FN fl/fl and ApoE −/− FN MxCre mice. Arrowheads point to immunosignals in ECs. Western blot for ICAM-1 in lysates from ECs cultured for 36 h in the presence or absence of pFN. Adhesion of leukocytes and inflammatory monocytes/neutrophils to carotid arteries of ApoE −/− FN fl/fl and ApoE −/− FN MxCre mice fed with high-fat diet for 4.5 weeks. Values are mean ± SD. Representative images of inflammatory monocytes/neutrophils adhering (arrowheads) to the luminal side of the external carotid artery in ApoE −/− FN fl/fl and ApoE −/− FN MxCre mice. Quantification of adhesion of Mac-1-positive cells on a monolayer of ECs under static condition and laminar shear (2.5 dyne/cm 2 ). Values are mean ± SD and normalized to adherent cells in the presence of FN in the culture medium; p = 0.025, 0.023 and 0.029.
Figure Legend Snippet: Reduced NF-κB and PAK activation and macrophage recruitment into atheroprone areas of ApoE −/− FN MxCre mice Immunostaining of phosho-NFκB/p65, phospho-PAK, phospho-JNK and ICAM-1 in the lesser curvature of the aortic arch from ApoE −/− FN fl/fl and ApoE −/− FN MxCre mice. Arrowheads point to immunosignals in ECs. Western blot for ICAM-1 in lysates from ECs cultured for 36 h in the presence or absence of pFN. Adhesion of leukocytes and inflammatory monocytes/neutrophils to carotid arteries of ApoE −/− FN fl/fl and ApoE −/− FN MxCre mice fed with high-fat diet for 4.5 weeks. Values are mean ± SD. Representative images of inflammatory monocytes/neutrophils adhering (arrowheads) to the luminal side of the external carotid artery in ApoE −/− FN fl/fl and ApoE −/− FN MxCre mice. Quantification of adhesion of Mac-1-positive cells on a monolayer of ECs under static condition and laminar shear (2.5 dyne/cm 2 ). Values are mean ± SD and normalized to adherent cells in the presence of FN in the culture medium; p = 0.025, 0.023 and 0.029.

Techniques Used: Activation Assay, Mouse Assay, Immunostaining, Western Blot, Cell Culture

29) Product Images from "Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages"

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

Journal: Journal of Virology

doi: 10.1128/JVI.77.18.10125-10130.2003

Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.
Figure Legend Snippet: Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.

Techniques Used: Infection, FACS, Expressing

MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.
Figure Legend Snippet: MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.

Techniques Used: Blocking Assay, Expressing, FACS, Infection

MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
Figure Legend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Techniques Used: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection

30) Product Images from "Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages"

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

Journal: Journal of Virology

doi: 10.1128/JVI.77.18.10125-10130.2003

Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.
Figure Legend Snippet: Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.

Techniques Used: Infection, FACS, Expressing

MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.
Figure Legend Snippet: MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.

Techniques Used: Blocking Assay, Expressing, FACS, Infection

MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
Figure Legend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Techniques Used: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection

31) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

32) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

33) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

34) Product Images from "Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus"

Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus

Journal: Journal of Virology

doi: 10.1128/JVI.75.20.9780-9789.2001

Expression of APC surface markers on unstimulated and IFN-γ-treated SJL microglia. Microglia cultures were unstimulated or stimulated with IFN-γ for 24 h. The microglia were stained with antibodies for Mac-1 (CD11b), CD45, ICAM-1 (CD54), CD40, B7-1 (CD80), B7-2 (CD86), MHC class II (I-A S ), and MHC class I (H-2K S ). Surface expression was then analyzed by flow cytometry, with the single line in each histogram representing the isotype antibody control and the solid peak representing the surface marker staining listed on the x axis. The flow plots shown represent all of the cells derived from each of the various culture conditions. These results are representative of four separate experiments.
Figure Legend Snippet: Expression of APC surface markers on unstimulated and IFN-γ-treated SJL microglia. Microglia cultures were unstimulated or stimulated with IFN-γ for 24 h. The microglia were stained with antibodies for Mac-1 (CD11b), CD45, ICAM-1 (CD54), CD40, B7-1 (CD80), B7-2 (CD86), MHC class II (I-A S ), and MHC class I (H-2K S ). Surface expression was then analyzed by flow cytometry, with the single line in each histogram representing the isotype antibody control and the solid peak representing the surface marker staining listed on the x axis. The flow plots shown represent all of the cells derived from each of the various culture conditions. These results are representative of four separate experiments.

Techniques Used: Expressing, Staining, Flow Cytometry, Cytometry, Marker, Derivative Assay

SJL microglia infected with TMEV upregulate expression of APC surface markers. Microglia were infected for 48 h with the BeAn strain of TMEV and then stained with antibodies for Mac-1, CD45, ICAM-1, CD40, B7-1, B7-2, MHC class II, and MHC class I. The single line in each histogram represents the isotype antibody control. The solid peak in each histogram represents the specific antibody staining for the surface markers listed on the x axis. These results are representative of four separate experiments.
Figure Legend Snippet: SJL microglia infected with TMEV upregulate expression of APC surface markers. Microglia were infected for 48 h with the BeAn strain of TMEV and then stained with antibodies for Mac-1, CD45, ICAM-1, CD40, B7-1, B7-2, MHC class II, and MHC class I. The single line in each histogram represents the isotype antibody control. The solid peak in each histogram represents the specific antibody staining for the surface markers listed on the x axis. These results are representative of four separate experiments.

Techniques Used: Infection, Expressing, Staining

35) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

36) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

37) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

38) Product Images from "Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells"

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.2001.01500.x

a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).
Figure Legend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

Techniques Used: Expressing, Staining, Cell Culture

39) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

40) Product Images from "Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo"

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

Journal: Journal of Clinical Investigation

doi:

Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
Figure Legend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

Techniques Used: Expressing, In Vitro, Transfection, FACS

Related Articles

Transfection:

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. Cells were harvested 72 h after transfection and tested for expression using FACS® analysis with FITC-conjugated monoclonal antibodies for ICAM-1, LFA-3, VCAM-1 (PharMingen, San Diego, California, USA) ( ). .. The quadriceps muscles of 6- to 8-week-old female BALB/c mice (Harlan Sprague Dawley Inc., Indianapolis, Indiana, USA) were injected with 50 μg of each DNA construct of interest formulated in PBS and 0.25% bupivacaine-HCl (Sigma Chemical Co., St. Louis, Missouri, USA).

In Vivo:

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. The effects of ICAM-1 and LFA-3 could be dependent on B7-CD28 signals, or they could represent an alternative synergistic pathway for driving CTL induction in vivo . .. We further investigated whether ICAM-1 and LFA-3 molecules when coexpressed with CD86 molecules could synergistically enhance the level of CTL induction.

Blocking Assay:

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages
Article Snippet: .. MCMV inhibition of TNF-α-induced ICAM-1 expression required live virus, since UV-inactivated MCMV did not block TNF-α induction of ICAM-1 and was specific to ICAM-1, as MCMV infection does not affect the surface expression of IFNGR1 ( ). .. Viral DNA synthesis and late gene expression were not required, since viral inhibition of TNF-α-induced ICAM-1 surface expression was not sensitive to phosphonoacetic acid at 400 μg/ml or cidofovir treatment at 2 μg/ml at the time of infection and at the time of stimulation (Fig. and data not shown) ( , ).

CTL Assay:

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. The effects of ICAM-1 and LFA-3 could be dependent on B7-CD28 signals, or they could represent an alternative synergistic pathway for driving CTL induction in vivo . .. We further investigated whether ICAM-1 and LFA-3 molecules when coexpressed with CD86 molecules could synergistically enhance the level of CTL induction.

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. It appears that LFA-3 has particular effects on class II responses, whereas in general, ICAM-1 was a dramatically strong driver of CTL induction and CD8+ effector function, as demonstrated by enhanced production of β-chemokines. ..

Inhibition:

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages
Article Snippet: .. MCMV inhibition of TNF-α-induced ICAM-1 expression required live virus, since UV-inactivated MCMV did not block TNF-α induction of ICAM-1 and was specific to ICAM-1, as MCMV infection does not affect the surface expression of IFNGR1 ( ). .. Viral DNA synthesis and late gene expression were not required, since viral inhibition of TNF-α-induced ICAM-1 surface expression was not sensitive to phosphonoacetic acid at 400 μg/ml or cidofovir treatment at 2 μg/ml at the time of infection and at the time of stimulation (Fig. and data not shown) ( , ).

Infection:

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages
Article Snippet: .. MCMV infection alone results in a modest up-regulation of ICAM-1 (Fig. ). .. Uninfected macrophages showed a robust up-regulation of surface ICAM-1 expression after TNF-α treatment (Fig. ).

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages
Article Snippet: .. MCMV inhibition of TNF-α-induced ICAM-1 expression required live virus, since UV-inactivated MCMV did not block TNF-α induction of ICAM-1 and was specific to ICAM-1, as MCMV infection does not affect the surface expression of IFNGR1 ( ). .. Viral DNA synthesis and late gene expression were not required, since viral inhibition of TNF-α-induced ICAM-1 surface expression was not sensitive to phosphonoacetic acid at 400 μg/ml or cidofovir treatment at 2 μg/ml at the time of infection and at the time of stimulation (Fig. and data not shown) ( , ).

Expressing:

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. Cells were harvested 72 h after transfection and tested for expression using FACS® analysis with FITC-conjugated monoclonal antibodies for ICAM-1, LFA-3, VCAM-1 (PharMingen, San Diego, California, USA) ( ). .. The quadriceps muscles of 6- to 8-week-old female BALB/c mice (Harlan Sprague Dawley Inc., Indianapolis, Indiana, USA) were injected with 50 μg of each DNA construct of interest formulated in PBS and 0.25% bupivacaine-HCl (Sigma Chemical Co., St. Louis, Missouri, USA).

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. Using DNA expression cassettes encoding for ICAM-1, LFA-3, and VCAM-1 along with our DNA immunogens, we sought to identify the specific effects of coexpressing adhesion molecules along with antigens. .. We observed that antigen-specific T-cell (both CD4+ and CD8+ T cells) responses can be enhanced by the coexpression of DNA immunogen and adhesion molecules ICAM-1 and LFA-3.

Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells
Article Snippet: .. In preliminary experiments, the expression of ICAM.1 was found essentially unchanged through long-term cultivation of established cell lines (data not shown). ..

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages
Article Snippet: .. MCMV inhibition of TNF-α-induced ICAM-1 expression required live virus, since UV-inactivated MCMV did not block TNF-α induction of ICAM-1 and was specific to ICAM-1, as MCMV infection does not affect the surface expression of IFNGR1 ( ). .. Viral DNA synthesis and late gene expression were not required, since viral inhibition of TNF-α-induced ICAM-1 surface expression was not sensitive to phosphonoacetic acid at 400 μg/ml or cidofovir treatment at 2 μg/ml at the time of infection and at the time of stimulation (Fig. and data not shown) ( , ).

FACS:

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. Cells were harvested 72 h after transfection and tested for expression using FACS® analysis with FITC-conjugated monoclonal antibodies for ICAM-1, LFA-3, VCAM-1 (PharMingen, San Diego, California, USA) ( ). .. The quadriceps muscles of 6- to 8-week-old female BALB/c mice (Harlan Sprague Dawley Inc., Indianapolis, Indiana, USA) were injected with 50 μg of each DNA construct of interest formulated in PBS and 0.25% bupivacaine-HCl (Sigma Chemical Co., St. Louis, Missouri, USA).

Binding Assay:

Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo
Article Snippet: .. As shown in Fig. a , coexpression of ICAM-1, LFA-3, or VCAM-1 appeared to have a minimal effect on the specific antibody binding profile induced by pCEnv immunization. ..

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    Pharmingen tnf α induced adhesion molecule icam 1
    Uninfected BM macrophages respond to <t>TNF-α</t> treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface <t>ICAM-1</t> expression. Results of one representative experiment of six independent experiments are shown.
    Tnf α Induced Adhesion Molecule Icam 1, supplied by Pharmingen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α induced adhesion molecule icam 1/product/Pharmingen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α induced adhesion molecule icam 1 - by Bioz Stars, 2020-09
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    92
    Pharmingen icam 1
    Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( <t>ICAM-1</t> ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.
    Icam 1, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.

    Journal: Journal of Virology

    Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

    doi: 10.1128/JVI.77.18.10125-10130.2003

    Figure Lengend Snippet: Uninfected BM macrophages respond to TNF-α treatment in the presence of infected BM macrophages. Shown is FACS analysis of BM macrophages for surface ICAM-1 expression. Results of one representative experiment of six independent experiments are shown.

    Article Snippet: Cells were harvested for fluorescence-activated cell sorter (FACS) analysis of the TNF-α-induced adhesion molecule ICAM-1 ( ) (Fig. ) by using the 3E2 monoclonal antibody specific for ICAM-1 (Pharmingen) ( ).

    Techniques: Infection, FACS, Expressing

    MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.

    Journal: Journal of Virology

    Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

    doi: 10.1128/JVI.77.18.10125-10130.2003

    Figure Lengend Snippet: MCMV IE and/or E genes block TNF-α-induced ICAM-1 expression. (a) FACS analysis of BM macrophages for surface ICAM-1 expression. (b) BM macrophages were infected and stimulated as done for panel a with the addition of phosphonoacetic acid (PAA) treatment. Isotype control is represented by filled histograms in panels a and b. Results of one representative experiment of three independent experiments are shown.

    Article Snippet: Cells were harvested for fluorescence-activated cell sorter (FACS) analysis of the TNF-α-induced adhesion molecule ICAM-1 ( ) (Fig. ) by using the 3E2 monoclonal antibody specific for ICAM-1 (Pharmingen) ( ).

    Techniques: Blocking Assay, Expressing, FACS, Infection

    MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

    Journal: Journal of Virology

    Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

    doi: 10.1128/JVI.77.18.10125-10130.2003

    Figure Lengend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

    Article Snippet: Cells were harvested for fluorescence-activated cell sorter (FACS) analysis of the TNF-α-induced adhesion molecule ICAM-1 ( ) (Fig. ) by using the 3E2 monoclonal antibody specific for ICAM-1 (Pharmingen) ( ).

    Techniques: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection

    Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

    Journal: Journal of Clinical Investigation

    Article Title: Intracellular adhesion molecule-1 modulates ?-chemokines and directly costimulates T cells in vivo

    doi:

    Figure Lengend Snippet: Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma ( RD ) cells in vitro . RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS ® analysis using either control antibodies, α–intracellular adhesion molecule-1 ( ICAM-1 ), or α–lymphocyte function associated-3 ( LFA-3 ) antibodies to detect expression of the transfected gene product.

    Article Snippet: The effects of ICAM-1 and LFA-3 could be dependent on B7-CD28 signals, or they could represent an alternative synergistic pathway for driving CTL induction in vivo .

    Techniques: Expressing, In Vitro, Transfection, FACS

    a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

    Journal: Clinical and Experimental Immunology

    Article Title: Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sj?gren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells

    doi: 10.1046/j.1365-2249.2001.01500.x

    Figure Lengend Snippet: a, Representative example of constitutive ICAM.1 expression (3+ staining intensity score, SI) by a SGEC line established from a patient with primary SS and cultured in SEM medium (Original magnification × 46). b, Low spontaneous ICAM.1 expression (SI: 1+) by a SEM-cultured SGEC line obtained from a control patient (Original magnification × 46). c, Down-regulation of spontaneous ICAM.1 expression by the SGEC line shown in (a), upon cultivation in the serum-free, low calcium-containing KBM medium. Only a small population of flattened-cuboidal epithelial cells (arrowheads) retains strong levels of ICAM.1 expression (Original magnification × 46). d, Induction of strong expression of ICAM.1 by the KBM-cultured SGEC line shown in (c), following treatment with IFNα (Original magnification × 46). e, Representative example of ICAM.1 expression and morphological features attained by SGEC upon transfer of cells from KBM to calcium-supplemented KBM culture medium (CS-KBM). Cultivation in CS-KBM restores the spontaneous expression of ICAM.1 at a significant degree (SI: 2+) and leads to the acquisition of morphological characteristics largely identical to those of cells cultured in SEM (tightly connected, large and flattened cells) (Original magnification × 46). f, Weak basal expression of ICAM.1 (SI: 1+) by a control SGEC line cultured in CS-KBM. Addition of calcium in KBM does not up-regulate the spontaneous ICAM.1 expression (Original magnification × 46).

    Article Snippet: In preliminary experiments, the expression of ICAM.1 was found essentially unchanged through long-term cultivation of established cell lines (data not shown).

    Techniques: Expressing, Staining, Cell Culture