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Ancell corporation icam 1
<t>ICAM-1</t> inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
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Images

1) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

2) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

3) Product Images from "Micromechanical Tests of Adhesion Dynamics between Neutrophils and Immobilized ICAM-1"

Article Title: Micromechanical Tests of Adhesion Dynamics between Neutrophils and Immobilized ICAM-1

Journal: Biophysical Journal

doi:

Time and temperature-dependent adhesion probability between ICAM-1 coated beads and neutrophils. Each bar represents data from 10 cell-bead pairs from each of three donors (total of 30 cell-bead pairs). For the 2-s contacts each cell-bead pair was contacted
Figure Legend Snippet: Time and temperature-dependent adhesion probability between ICAM-1 coated beads and neutrophils. Each bar represents data from 10 cell-bead pairs from each of three donors (total of 30 cell-bead pairs). For the 2-s contacts each cell-bead pair was contacted

Techniques Used:

Immunofluorescent flow cytometry analysis of ICAM-1 coated beads. ( A ) The left peak corresponds to the antibody-binding capacity of nonspecific IgG; the right peak corresponds to the binding capacity of antibody against ICAM-1. ( B ) Calibration curve to
Figure Legend Snippet: Immunofluorescent flow cytometry analysis of ICAM-1 coated beads. ( A ) The left peak corresponds to the antibody-binding capacity of nonspecific IgG; the right peak corresponds to the binding capacity of antibody against ICAM-1. ( B ) Calibration curve to

Techniques Used: Flow Cytometry, Cytometry, Binding Assay

Effect of blocking antibodies on neutrophil adhesion to ICAM-1. Experiments were performed in 1.5 mM Ca 2+ or 5.0 mM Mg 2+ plus EGTA. The number of binding sites for ICAM-1 was corrected to 290/ μ m 2 and the contact area was corrected
Figure Legend Snippet: Effect of blocking antibodies on neutrophil adhesion to ICAM-1. Experiments were performed in 1.5 mM Ca 2+ or 5.0 mM Mg 2+ plus EGTA. The number of binding sites for ICAM-1 was corrected to 290/ μ m 2 and the contact area was corrected

Techniques Used: Blocking Assay, Binding Assay

Adhesion probability between neutrophils and beads coated with ICAM-1. Each ICAM-1 column represents data from 105 cell-bead pairs total from seven donors, and the NCAM column represents 24 cell-bead pairs from three donors. Experiments were performed
Figure Legend Snippet: Adhesion probability between neutrophils and beads coated with ICAM-1. Each ICAM-1 column represents data from 105 cell-bead pairs total from seven donors, and the NCAM column represents 24 cell-bead pairs from three donors. Experiments were performed

Techniques Used:

4) Product Images from "Micromechanical Tests of Adhesion Dynamics between Neutrophils and Immobilized ICAM-1"

Article Title: Micromechanical Tests of Adhesion Dynamics between Neutrophils and Immobilized ICAM-1

Journal: Biophysical Journal

doi:

Time and temperature-dependent adhesion probability between ICAM-1 coated beads and neutrophils. Each bar represents data from 10 cell-bead pairs from each of three donors (total of 30 cell-bead pairs). For the 2-s contacts each cell-bead pair was contacted
Figure Legend Snippet: Time and temperature-dependent adhesion probability between ICAM-1 coated beads and neutrophils. Each bar represents data from 10 cell-bead pairs from each of three donors (total of 30 cell-bead pairs). For the 2-s contacts each cell-bead pair was contacted

Techniques Used:

Immunofluorescent flow cytometry analysis of ICAM-1 coated beads. ( A ) The left peak corresponds to the antibody-binding capacity of nonspecific IgG; the right peak corresponds to the binding capacity of antibody against ICAM-1. ( B ) Calibration curve to
Figure Legend Snippet: Immunofluorescent flow cytometry analysis of ICAM-1 coated beads. ( A ) The left peak corresponds to the antibody-binding capacity of nonspecific IgG; the right peak corresponds to the binding capacity of antibody against ICAM-1. ( B ) Calibration curve to

Techniques Used: Flow Cytometry, Cytometry, Binding Assay

Effect of blocking antibodies on neutrophil adhesion to ICAM-1. Experiments were performed in 1.5 mM Ca 2+ or 5.0 mM Mg 2+ plus EGTA. The number of binding sites for ICAM-1 was corrected to 290/ μ m 2 and the contact area was corrected
Figure Legend Snippet: Effect of blocking antibodies on neutrophil adhesion to ICAM-1. Experiments were performed in 1.5 mM Ca 2+ or 5.0 mM Mg 2+ plus EGTA. The number of binding sites for ICAM-1 was corrected to 290/ μ m 2 and the contact area was corrected

Techniques Used: Blocking Assay, Binding Assay

Adhesion probability between neutrophils and beads coated with ICAM-1. Each ICAM-1 column represents data from 105 cell-bead pairs total from seven donors, and the NCAM column represents 24 cell-bead pairs from three donors. Experiments were performed
Figure Legend Snippet: Adhesion probability between neutrophils and beads coated with ICAM-1. Each ICAM-1 column represents data from 105 cell-bead pairs total from seven donors, and the NCAM column represents 24 cell-bead pairs from three donors. Experiments were performed

Techniques Used:

5) Product Images from "Signaling and Dynamics of Activation of LFA-1 and Mac-1 by Immobilized IL-8"

Article Title: Signaling and Dynamics of Activation of LFA-1 and Mac-1 by Immobilized IL-8

Journal: Cellular and molecular bioengineering

doi: 10.1007/s12195-009-0099-x

Effect on intracellular and extracellular calcium chelation on neutrophils adhesion to ICAM-1. A . Inhibition of calcium release by 10 mM BAPTA. B . Inhibition of IL-8 induced neutrophils adhesion to ICAM-1 by 10 µM BAPTA or 0.5 mM EGTA. Experiments
Figure Legend Snippet: Effect on intracellular and extracellular calcium chelation on neutrophils adhesion to ICAM-1. A . Inhibition of calcium release by 10 mM BAPTA. B . Inhibition of IL-8 induced neutrophils adhesion to ICAM-1 by 10 µM BAPTA or 0.5 mM EGTA. Experiments

Techniques Used: Inhibition

Effect of immobilized IL-8 (control), E-selectin or anti-CD45 antibody on neutrophil adhesion to ICAM-1. IL-8 was immobilized on the protein G coated beads at 1,800 sites/µm 2 and E-selectin was immobilized on tosyl-activated beads at 9,000 sites/µm
Figure Legend Snippet: Effect of immobilized IL-8 (control), E-selectin or anti-CD45 antibody on neutrophil adhesion to ICAM-1. IL-8 was immobilized on the protein G coated beads at 1,800 sites/µm 2 and E-selectin was immobilized on tosyl-activated beads at 9,000 sites/µm

Techniques Used:

Effect of signaling pathways inhibitors on IL-8 induced neutrophil adhesion to ICAM-1. A. PI3k inhibitor wortmannin (500 nM) and B. PKC inhibitor BIM (1µM) had no effect. C.
Figure Legend Snippet: Effect of signaling pathways inhibitors on IL-8 induced neutrophil adhesion to ICAM-1. A. PI3k inhibitor wortmannin (500 nM) and B. PKC inhibitor BIM (1µM) had no effect. C.

Techniques Used:

Effect of IP3 inhibitors caffeine and 2APB on IL-8 induced calcium release and neutrophil binding to ICAM-1. A . Inhibition of calcium spark by caffeine. While cells pre-incubation with 5mM caffeine was partially effective, 10mM caffeine completely inhibited
Figure Legend Snippet: Effect of IP3 inhibitors caffeine and 2APB on IL-8 induced calcium release and neutrophil binding to ICAM-1. A . Inhibition of calcium spark by caffeine. While cells pre-incubation with 5mM caffeine was partially effective, 10mM caffeine completely inhibited

Techniques Used: Binding Assay, Inhibition, Incubation

Time dependent IL-8 induced neutrophil adhesion to ICAM-1. Experiments were performed in the presence of A. lovastatin (100 µM); B. Mac-1 blocking antibodies MEM174 and ICRF44 (each at 15 µl/ml); C . MAPK inhibitor SB203580 (15 µM).
Figure Legend Snippet: Time dependent IL-8 induced neutrophil adhesion to ICAM-1. Experiments were performed in the presence of A. lovastatin (100 µM); B. Mac-1 blocking antibodies MEM174 and ICRF44 (each at 15 µl/ml); C . MAPK inhibitor SB203580 (15 µM).

Techniques Used: Blocking Assay

Series of video microphotographs showing the experimental setup for the micropipette experiment. A. Initial setup. Left pipette is holding ICAM-1 coated bead (4.5µm in diameter) and right pipette is holding human neutrophil. During the experiment
Figure Legend Snippet: Series of video microphotographs showing the experimental setup for the micropipette experiment. A. Initial setup. Left pipette is holding ICAM-1 coated bead (4.5µm in diameter) and right pipette is holding human neutrophil. During the experiment

Techniques Used: Transferring

6) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

7) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

8) Product Images from "Interplay between Shear Stress and Adhesion on Neutrophil Locomotion"

Article Title: Interplay between Shear Stress and Adhesion on Neutrophil Locomotion

Journal: Biophysical Journal

doi: 10.1529/biophysj.105.079418

Percentage of cells with a net movement in the direction of flow. ( A ) The percentage of cells with a positive index of migration ( x f ) in the direction of shear was found for surfaces coated in 0.1 μ g/ml E-selectin ( solid bars , low-E-selectin), 1 μ g/ml E-selectin ( black dots , E-selectin), 1 μ g/ml E-selectin + 0.5 μ g/ml PECAM-1 ( horizontal black stripes , low PECAM-1), 1 μ g/ml E-selectin + 1 μ g/ml PECAM-1 ( diagonal black stripes , moderate-PECAM-1), 1 μ g/ml E-selectin + 4 μ g/ml PECAM-1 ( vertical black stripes , high-PECAM-1), and 1 μ g/ml E-selectin + 4 μ g/ml ICAM-1 ( checkered , ICAM-1) at 0 ( first grouping ), 180 ( second grouping ), and 1000 s −1 ( third grouping ). The average percentages are found from at least 4 d, and the error bars represent standard error. A two-way ANOVA and a least-squares means differences Tukey HSD test were performed using JMP 5.1 with α = 0.05 (no statistically significant differences were found).
Figure Legend Snippet: Percentage of cells with a net movement in the direction of flow. ( A ) The percentage of cells with a positive index of migration ( x f ) in the direction of shear was found for surfaces coated in 0.1 μ g/ml E-selectin ( solid bars , low-E-selectin), 1 μ g/ml E-selectin ( black dots , E-selectin), 1 μ g/ml E-selectin + 0.5 μ g/ml PECAM-1 ( horizontal black stripes , low PECAM-1), 1 μ g/ml E-selectin + 1 μ g/ml PECAM-1 ( diagonal black stripes , moderate-PECAM-1), 1 μ g/ml E-selectin + 4 μ g/ml PECAM-1 ( vertical black stripes , high-PECAM-1), and 1 μ g/ml E-selectin + 4 μ g/ml ICAM-1 ( checkered , ICAM-1) at 0 ( first grouping ), 180 ( second grouping ), and 1000 s −1 ( third grouping ). The average percentages are found from at least 4 d, and the error bars represent standard error. A two-way ANOVA and a least-squares means differences Tukey HSD test were performed using JMP 5.1 with α = 0.05 (no statistically significant differences were found).

Techniques Used: Flow Cytometry, Migration

Index of migration. ( A ) The index of migration ( x f / d ) was found for surfaces coated in 0.1 μ g/ml E-selectin ( solid bars , low-E-selectin), 1 μ g/ml E-selectin ( black dots , E-selectin), 1 μ g/ml E-selectin + 0.5 μ g/ml PECAM-1 ( horizontal black stripes , low-PECAM-1), 1 μ g/ml E-selectin + 1 μ g/ml PECAM-1 ( diagonal black stripes , moderate-PECAM-1), 1 μ g/ml E-selectin + 4 μ g/ml PECAM-1 ( vertical black stripes , high-PECAM-1), and 1 μ g/ml E-selectin + 4 μ g/ml ICAM-1 ( checkered , ICAM-1) at 0 ( first grouping ), 180 ( second grouping ), and 1000 s −1 ( third grouping ). The average index of migration is found from at least 4 d, and the error bars represent standard error. A two-way ANOVA and a least-squares means differences Tukey HSD test were performed using JMP 5.1 with α = 0.05 ( # is statistically different from , and † is statistically different from ‡ ).
Figure Legend Snippet: Index of migration. ( A ) The index of migration ( x f / d ) was found for surfaces coated in 0.1 μ g/ml E-selectin ( solid bars , low-E-selectin), 1 μ g/ml E-selectin ( black dots , E-selectin), 1 μ g/ml E-selectin + 0.5 μ g/ml PECAM-1 ( horizontal black stripes , low-PECAM-1), 1 μ g/ml E-selectin + 1 μ g/ml PECAM-1 ( diagonal black stripes , moderate-PECAM-1), 1 μ g/ml E-selectin + 4 μ g/ml PECAM-1 ( vertical black stripes , high-PECAM-1), and 1 μ g/ml E-selectin + 4 μ g/ml ICAM-1 ( checkered , ICAM-1) at 0 ( first grouping ), 180 ( second grouping ), and 1000 s −1 ( third grouping ). The average index of migration is found from at least 4 d, and the error bars represent standard error. A two-way ANOVA and a least-squares means differences Tukey HSD test were performed using JMP 5.1 with α = 0.05 ( # is statistically different from , and † is statistically different from ‡ ).

Techniques Used: Migration

Migration velocity. ( A ) Migration velocities for surfaces coated in 0.1 μ g/ml E-selectin ( solid bars , low-E-selectin), 1 μ g/ml E-selectin ( black dots , E-selectin), 1 μ g/ml E-selectin + 0.5 μ g/ml PECAM-1 ( horizontal black stripes , low-PECAM-1), 1 μ g/ml E-selectin + 1 μ g/ml PECAM-1 ( diagonal black stripes , moderate-PECAM-1), 1 μ g/ml E-selectin + 4 μ g/ml PECAM-1 ( vertical black stripes , high-PECAM-1), and 1 μ g/ml E-selectin + 4 μ g/ml ICAM-1, ( checkered , ICAM-1) at 0 ( first grouping ), 180 ( second grouping ), and 1000 s −1 ( third grouping ). The velocities were found by dividing the contour length of the cell trajectory during the migration phase ( d ) by the time the cell is in its migration regime. The migration velocities are found from at least 4 d, and the error bars represent standard error. A two-way ANOVA and a least-squares means differences Tukey HSD test were performed using JMP 5.1 with α = 0.05 ( # is statistically different from , and † is statistically different from ‡ ).
Figure Legend Snippet: Migration velocity. ( A ) Migration velocities for surfaces coated in 0.1 μ g/ml E-selectin ( solid bars , low-E-selectin), 1 μ g/ml E-selectin ( black dots , E-selectin), 1 μ g/ml E-selectin + 0.5 μ g/ml PECAM-1 ( horizontal black stripes , low-PECAM-1), 1 μ g/ml E-selectin + 1 μ g/ml PECAM-1 ( diagonal black stripes , moderate-PECAM-1), 1 μ g/ml E-selectin + 4 μ g/ml PECAM-1 ( vertical black stripes , high-PECAM-1), and 1 μ g/ml E-selectin + 4 μ g/ml ICAM-1, ( checkered , ICAM-1) at 0 ( first grouping ), 180 ( second grouping ), and 1000 s −1 ( third grouping ). The velocities were found by dividing the contour length of the cell trajectory during the migration phase ( d ) by the time the cell is in its migration regime. The migration velocities are found from at least 4 d, and the error bars represent standard error. A two-way ANOVA and a least-squares means differences Tukey HSD test were performed using JMP 5.1 with α = 0.05 ( # is statistically different from , and † is statistically different from ‡ ).

Techniques Used: Migration

9) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

10) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

11) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

12) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

13) Product Images from "Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling"

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

Journal: Infection and Immunity

doi:

ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

Techniques Used: Expressing, Incubation, Northern Blot, Inhibition

ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.
Figure Legend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

Techniques Used: Binding Assay, Labeling, Inhibition

Related Articles

Binding Assay:

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling
Article Snippet: .. ICAM-1 is a ligand for CD11/CD18 and has five Ig-like domains; domains 3 through 5 are essential for the binding of ICAM-1 to integrins. .. With this fact in mind, we examined whether the fimbriae have amino acid sequence homology with domains 3 through 5 of ICAM-1 and found that fimbrillin contains some regions in its amino acid sequence homologous to some sites in domains 3 through 5 of ICAM-1.

Article Title: Micromechanical Tests of Adhesion Dynamics between Neutrophils and Immobilized ICAM-1
Article Snippet: .. MAb38 (anti-human CD11a, IgG2a), which recognizes the I domain of the integrin α L -subunit (CD11a) and blocks binding of ICAM-1 to LFA-1, and mAb IB4 (anti-human CD18, IgG2a), which recognizes the β 2 -integrin subunit (CD18) and blocks binding to ICAM-1 were purchased from Ancell (Bayport, MN). .. MAb CBRM1/5 (anti-human CD11b) was purchased from eBioscience (San Diego, CA).

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling
Article Snippet: .. As we expected, ICAM-1 (Ancell Corp., Bayport, Minn.) indeed inhibited fimbrial binding to cells in a dose-dependent manner (Fig. A). .. However, laminin (Koken Co., Tokyo, Japan), a ligand of β1 integrin, did not inhibit fimbrial binding to cells (Fig. B).

Blocking Assay:

Article Title: Interplay between Shear Stress and Adhesion on Neutrophil Locomotion
Article Snippet: .. Following blocking procedures, mouse anti-human E-selectin, ICAM-1, or PECAM-1 antibody (Ancell) was added at 2 μ g/ml for 2 h. After three PBS washes, horseradish peroxidase (HRP)-conjugated rat anti-mouse secondary antibody (BD Pharmingen, San Diego, CA) was added at 1:1000 dilution for 2 h. Samples were then washed three more times with PBS before addition of Amplex Red ELISA substrate (Molecular Probes, Eugene, OR). .. Measurements were made after 30 min using a fluorescence plate reader with 485 nm excitation and 525 nm emission filters.

Enzyme-linked Immunosorbent Assay:

Article Title: Interplay between Shear Stress and Adhesion on Neutrophil Locomotion
Article Snippet: .. Following blocking procedures, mouse anti-human E-selectin, ICAM-1, or PECAM-1 antibody (Ancell) was added at 2 μ g/ml for 2 h. After three PBS washes, horseradish peroxidase (HRP)-conjugated rat anti-mouse secondary antibody (BD Pharmingen, San Diego, CA) was added at 1:1000 dilution for 2 h. Samples were then washed three more times with PBS before addition of Amplex Red ELISA substrate (Molecular Probes, Eugene, OR). .. Measurements were made after 30 min using a fluorescence plate reader with 485 nm excitation and 525 nm emission filters.

Incubation:

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling
Article Snippet: .. Therefore, the cells were pretreated or not pretreated with ICAM-1 at 50 U/ml for 3 h before the addition of fimbriae and then were incubated for 1 h in the absence or presence of fimbriae. .. As shown in Fig. , ICAM-1 at 50 U/ml clearly inhibited the fimbria-induced expression of the IL-1β and TNF-α genes in cells.

Expressing:

Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling
Article Snippet: .. As shown in Fig. , ICAM-1 at 50 U/ml clearly inhibited the fimbria-induced expression of the IL-1β and TNF-α genes in cells. ..

Recombinant:

Article Title: Signaling and Dynamics of Activation of LFA-1 and Mac-1 by Immobilized IL-8
Article Snippet: .. Monoclonal antibodies against CD11b (clone ICRF44), ICAM-1 (clone 15.2) and CD45 (a protein tyrosine phosphatase, also known as the leukocyte common antigen) (clone C11) were purchased from Ancell (Bayport, MN), mAbs against IL-8 (clone 6217), human E-selectin (clone BBIG-E5) and recombinant human E-selectin were purchased from R & D Systems (Minneapolis, MN), IgG1 isotype control was obtained from Beckman Coulter Immunotech (Miami, Fl). .. All antibodies used for flow cytometry were FITC conjugated.

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    Ancell corporation icam 1
    <t>ICAM-1</t> inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.
    Icam 1, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ancell corporation mouse anti human icam 1 monoclonal igg1 antibody
    Binding density of <t>anti-ICAM-1</t> and negative control (Goat <t>IgG</t> control) antibody coated (A) 210 nm particles and (B) 2 μm particles under 200 s −1 shear rate for both pure buffer and RBC 25% flow cases.
    Mouse Anti Human Icam 1 Monoclonal Igg1 Antibody, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ancell corporation anti human icam 1
    Effect of chloride substitutes on neutrophil adhesion to <t>ICAM-1.</t> Experiments were performed in BSS, in low Cl - medium (glucuronate or glutamate), or in low Cl - medium in the presence of mAb38 (LFA-1 blocking antibody), ICRF44 (Mac-1 blocking antibody), IB4 (β 2 blocking antibody) or 100 μM NPPB as indicated. Each cell-bead pair was contacted 25 times for 2 seconds each time. Adhesion was detected as described in Materials and Methods, and adhesion probability was calculated from the number of adhesive events divided by the number of contacts. Each column represents the mean value from experiments on 4-6 cells from each of 2-4 different donors. Error bars represent s.e.m.
    Anti Human Icam 1, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human icam 1/product/Ancell corporation
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    ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

    Journal: Infection and Immunity

    Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

    doi:

    Figure Lengend Snippet: ICAM-1 inhibits the fimbria-induced expression of IL-1β and TNF-α genes in peritoneal macrophages. (A) Peritoneal macrophages in each well of a microculture plate were pretreated or not pretreated for 3 h with ICAM-1 at 50 U/ml and then incubated for 1 h with or without fimbriae (4 μg of protein/ml). After incubation, total RNAs were prepared, and Northern blot analysis was performed with IL-1β, TNF-α, and β-actin cDNAs as probes. (B) Quantification of IL-1β and TNF-α gene expression in panel A was done by densitometry, and the results are expressed as percent inhibition. An identical experiment performed independently gave similar results.

    Article Snippet: Therefore, the cells were pretreated or not pretreated with ICAM-1 at 50 U/ml for 3 h before the addition of fimbriae and then were incubated for 1 h in the absence or presence of fimbriae.

    Techniques: Expressing, Incubation, Northern Blot, Inhibition

    ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

    Journal: Infection and Immunity

    Article Title: Porphyromonas gingivalis Fimbriae Use ?2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 ? Chain Plays a Functional Role in Fimbrial Signaling

    doi:

    Figure Lengend Snippet: ICAM-1 inhibits the binding of 125 I-labeled fimbriae to mouse peritoneal macrophages. 125 I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125 I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125 I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.

    Article Snippet: Therefore, the cells were pretreated or not pretreated with ICAM-1 at 50 U/ml for 3 h before the addition of fimbriae and then were incubated for 1 h in the absence or presence of fimbriae.

    Techniques: Binding Assay, Labeling, Inhibition

    Binding density of anti-ICAM-1 and negative control (Goat IgG control) antibody coated (A) 210 nm particles and (B) 2 μm particles under 200 s −1 shear rate for both pure buffer and RBC 25% flow cases.

    Journal: Microvascular research

    Article Title: Characterization of nanoparticle delivery in microcirculation using a microfluidic device

    doi: 10.1016/j.mvr.2014.04.008

    Figure Lengend Snippet: Binding density of anti-ICAM-1 and negative control (Goat IgG control) antibody coated (A) 210 nm particles and (B) 2 μm particles under 200 s −1 shear rate for both pure buffer and RBC 25% flow cases.

    Article Snippet: Mouse anti-human ICAM-1 monoclonal IgG1 antibody (clone 15.2) was got from Ancell, Bayport, MN and HRP-conjugated rat anti-mouse IgG1 monoclonal antibody from BD Biosciences San Jose, CA.

    Techniques: Binding Assay, Negative Control, Flow Cytometry

    Effect of chloride substitutes on neutrophil adhesion to ICAM-1. Experiments were performed in BSS, in low Cl - medium (glucuronate or glutamate), or in low Cl - medium in the presence of mAb38 (LFA-1 blocking antibody), ICRF44 (Mac-1 blocking antibody), IB4 (β 2 blocking antibody) or 100 μM NPPB as indicated. Each cell-bead pair was contacted 25 times for 2 seconds each time. Adhesion was detected as described in Materials and Methods, and adhesion probability was calculated from the number of adhesive events divided by the number of contacts. Each column represents the mean value from experiments on 4-6 cells from each of 2-4 different donors. Error bars represent s.e.m.

    Journal: Blood cells, molecules & diseases

    Article Title: Activation of human neutrophil Mac-1 by anion substitution *

    doi: 10.1016/j.bcmd.2009.01.006

    Figure Lengend Snippet: Effect of chloride substitutes on neutrophil adhesion to ICAM-1. Experiments were performed in BSS, in low Cl - medium (glucuronate or glutamate), or in low Cl - medium in the presence of mAb38 (LFA-1 blocking antibody), ICRF44 (Mac-1 blocking antibody), IB4 (β 2 blocking antibody) or 100 μM NPPB as indicated. Each cell-bead pair was contacted 25 times for 2 seconds each time. Adhesion was detected as described in Materials and Methods, and adhesion probability was calculated from the number of adhesive events divided by the number of contacts. Each column represents the mean value from experiments on 4-6 cells from each of 2-4 different donors. Error bars represent s.e.m.

    Article Snippet: Monoclonal antibodies ICRF44 (anti-human CD11b, IgG1), which binds to the αM subunit and blocks Mac-1 ligand binding, R-PE-labeled and unlabeled mAb38 (anti-CD11a, IgG2a), which binds to the αL subunit and blocks LFA-1 ligand binding, R-PE-labeled and unlabeled IB4 (anti-CD18, IgG2a), which binds to the β2 subunit and blocks CD18 ligand binding, R-PE-labeled IgG2a isotype control and 15.2 (anti-human ICAM-1, IgG1) were purchased from Ancell (Bayport, MN).

    Techniques: Blocking Assay

    Interaction between a neutrophil and an ICAM-1 coated bead during the adhesion experiment: A . An ICAM-1 coated bead (4.5 μm diameter) is held in the left pipette and the neutrophil is held in the right pipette. B . The contact between the beads and the cell. Adhesion events are scored when there is a small deflection of the cell surface as the cell and bead are separated. The adhesion probability is calculated as the number of adhesive events over the total number of touches.

    Journal: Blood cells, molecules & diseases

    Article Title: Activation of human neutrophil Mac-1 by anion substitution *

    doi: 10.1016/j.bcmd.2009.01.006

    Figure Lengend Snippet: Interaction between a neutrophil and an ICAM-1 coated bead during the adhesion experiment: A . An ICAM-1 coated bead (4.5 μm diameter) is held in the left pipette and the neutrophil is held in the right pipette. B . The contact between the beads and the cell. Adhesion events are scored when there is a small deflection of the cell surface as the cell and bead are separated. The adhesion probability is calculated as the number of adhesive events over the total number of touches.

    Article Snippet: Monoclonal antibodies ICRF44 (anti-human CD11b, IgG1), which binds to the αM subunit and blocks Mac-1 ligand binding, R-PE-labeled and unlabeled mAb38 (anti-CD11a, IgG2a), which binds to the αL subunit and blocks LFA-1 ligand binding, R-PE-labeled and unlabeled IB4 (anti-CD18, IgG2a), which binds to the β2 subunit and blocks CD18 ligand binding, R-PE-labeled IgG2a isotype control and 15.2 (anti-human ICAM-1, IgG1) were purchased from Ancell (Bayport, MN).

    Techniques: Transferring