Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left panel top image: 3D intracardiac image and CT simulation demonstrating inadequate right atrial height and a deep capsule position. CT predicted adequate right atrial height of >60 mm. However, the large EV prevented adequate right atrial height and the capsule was too deep. Multiple maneuvers to gain height were insufficient from the right femoral approach, and we converted to a left femoral venous approach. Despite the left femoral approach, the capsule was still deep in the RV at 3.3 cm from the TV annulus and 2.3 cm from the systolic coaptation plane. Left panel bottom image: CT simulation demonstrating the position of the capsule in relation to the EV (∗) and the TV systolic coaptation. Right image: Capsule deep in the RV. The white interrupted line represents the approximate plane of the TV annulus. Abbreviations: A, anterior leaflet; CT, computed tomography; EV, Eustachian valve; P, posterior leaflet; RV, right ventricle; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques: Computed Tomography

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left image: ICE showing the PA trajectory with the posterior aspect of the capsule edge just beneath the systolic coaptation plane. Right image: Capsule gap created with a pronounced PA trajectory to gain RA height. The white interrupted line represents the approximate plane of the TV annulus. Abbreviations: ICE, intracardiac echo; PA, posterior-to-anterior; RA, right atrium; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left image: ICE multiplanar reconstruction images. The anchors are peeking out of the capsule with a pronounced anterior trajectory and the tip of the capsule at the anterior septal commissure in the RVOT. Right image: The entire system is retracted posteriorly to avoid chordal entanglement while maintaining the anterior trajectory. Abbreviations: A, anterior; ICE, intracardiac echo; L, lateral; P, posterior; RVOT, right ventricle outflow tract; S, septal.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Top panel: Progressive exposure of the anchors and ventricular flip of the anchors while simultaneously adding primary flex to correct the anterior trajectory and become coaxial to the TV annular plane. The white interrupted line represents the approximate plane of the TV annulus. Bottom panel: ICE multiplanar reconstruction images demonstrating the progressive ventricular flip of the EVOQUE anchors. Abbreviation: ICE, intracardiac echo; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left image: ICE image of the valve deployed. Right image: Fluoroscopy of the valve deployed. The white interrupted line represents the approximate plane of the TV annulus. Abbreviation: ICE, intracardiac echo; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Molecular Therapy Oncology

Article Title: Mevalonate pathway activation in Ewing sarcoma reveals a 3D-specific synergy between statins and BCL-xL inhibition

doi: 10.1016/j.omton.2026.201229

Figure Lengend Snippet: Hydrogel-based 3D culture system supports long-term Ewing sarcoma growth and drug testing (A) Schematic of the embedding process for creating 3D-constructs: ES cells are embedded in photocross-linkable hydrogels and polymerized to form cell-laden constructs. (B) STA-ET-1 cells embedded in gelNB92, 7.5% hydrogel with equimolar concentrations of cross-linker dithiothreitol (DTT) and 0.1 mM Li-TPO-L as a photoinitiator and cultured for 14 days as 3D-constructs. Cell viability was assessed using LIVE/DEAD staining, with green fluorescence indicating live cells, and red fluorescence indicating dead cells. (C) ES TC71 cells 48 h post-embedding, a critical early period for assessing viability and structural integrity, in gelNB92 (7.5%) hydrogel crosslinked with equimolar DTT and 0.5 mM DAS as a photoinitiator. Constructs were fabricated via two-photon polymerization (2PP) at 90 mW (left) and 100 mW (right), demonstrating compatibility for cell-laden bioprinting. (D) Schematic overview of RNA-seq sample comparisons across 2D cultures, 3D spheroids, and 3D constructs at multiple time points. (E) KEGG pathway enrichment analysis reveals further upregulation of the mevalonate pathway in encapsulated ES cultures compared to 2D (fgsea, FDR ≤0.005). Asterisks denote statistical significance based on adjusted p values: p < 0.05 (∗ ) , p < 0.01 (∗∗) , p < 0.001 ( ∗∗∗). (F) RT-qPCR data showing differences in gene expression between cells cultured in standard 2D conditions and the same cells cultured in 3D-constructs for 14 days. Each of the three 3D biological replicates combines 5 individual constructs. (G) ES PDX-derived cells (IC-pPDX-87) embedded in gelNB92, 7.5% hydrogel with equimolar concentrations of crosslinker dithiothreitol (DTT) and 0.1 mM Li-TPO-L remained viable and formed expanding spheroids over time, as confirmed by LIVE/DEAD staining (green: live cells, red: dead). Cells spontaneously assembled into spheroids within the hydrogel constructs, similar to their behavior in liquid culture. Three-dimensional projection analysis between day 1 and day 23 showed a reduction in the number of embedded spheroids, while those that persisted increased in size; a small number of viable spheroids also appeared in the surrounding medium. (H) Targeted dose-response analysis of doxorubicin in PDX-derived 3D-constructs. Three concentrations, selected from prior cell line spheroid screening, were tested in hydrogel-embedded constructs formed from dissociated IC-pPDX-87 cells. Cell viability was assessed after 72 h of treatment using luminescence assays. Each data point represents a single construct (one per well); three constructs were tested per concentration.

Article Snippet: IC-pPDX-87 was generated at Institut Curie from patients under an Institutional Review Board-approved protocol (OBS170323CPP ref. 3272; dossier no. 2015-A00464-45).

Techniques: Construct, Cell Culture, Staining, Fluorescence, RNA Sequencing, Quantitative RT-PCR, Gene Expression, Derivative Assay, Concentration Assay