ibmx  (Thermo Fisher)


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    Name:
    IBMX
    Description:
    IBMX is a nonspecific inhibitor of phosphodiesterases that also possesses adenosine agonist activity Format Formulation Lyophilized
    Catalog Number:
    phz1124
    Price:
    None
    Applications:
    Cell Analysis|Cell Culture|Cell Signaling|General Protein & Enzyme Activity Reagents|Mammalian Cell Culture|Protein Biology|Cell Viability, Proliferation & Function
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher ibmx
    Continuous exposure to albuterol reduces stimulated <t>CFTR</t> activity in a fashion similar to continuous or intermittent dosing with formoterol. wtCFTR + and VX809-corrected F508del-CFTR + CFBE41o- cells were exposed to either continuous or intermittent (1 hour, twice daily) albuterol (10 μM) or the long-acting β2AR-agonist formoterol (LABA; 1 μM) for 72 hours. Cells were mounted in Ussing chambers and CFTR function was assessed under voltage clamp conditions. ( A ) Representative short-circuit current (I sc ) tracings in wtCFTR + CFBE41o- cells exposed for 72 hours to either continuous (C) or intermittent (I) albuterol or formoterol; aggregate data are presented in B ( n = 4 inserts/condition; circles represent total CFTR activity [cAMP + genistein], diamonds represent inhibited CFTR currents [Inh172]). ( C ) Representative I sc tracings in VX809-treated F508del-CFTR + CFBE41o- cells exposed for 72 hours to either continuous (C) or intermittent (I) albuterol or formoterol; aggregate data are presented in D ( n = 4 inserts/condition). All data were normalized to the control or VX809 (no albuterol pretreatment) condition. Stimulation protocol was as follows: amiloride (100 μM, not shown), cAMP (10 μM forskolin/100 μM <t>IBMX;</t> dark gray bars), CFTR potentiator (50 μM genistein for wtCFTR + , 1 μM VX770 for F508del-CFTR + cells; light gray bars), and CFTR inhibition (10 μM Inh172; white bars). All experiments are representative of studies repeated in duplicate or triplicate with similar results. Data presented represent the mean ± SEM. * P
    IBMX is a nonspecific inhibitor of phosphodiesterases that also possesses adenosine agonist activity Format Formulation Lyophilized
    https://www.bioz.com/result/ibmx/product/Thermo Fisher
    Average 95 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    ibmx - by Bioz Stars, 2020-09
    95/100 stars

    Images

    1) Product Images from "Chronic β2AR stimulation limits CFTR activation in human airway epithelia"

    Article Title: Chronic β2AR stimulation limits CFTR activation in human airway epithelia

    Journal: JCI Insight

    doi: 10.1172/jci.insight.93029

    Continuous exposure to albuterol reduces stimulated CFTR activity in a fashion similar to continuous or intermittent dosing with formoterol. wtCFTR + and VX809-corrected F508del-CFTR + CFBE41o- cells were exposed to either continuous or intermittent (1 hour, twice daily) albuterol (10 μM) or the long-acting β2AR-agonist formoterol (LABA; 1 μM) for 72 hours. Cells were mounted in Ussing chambers and CFTR function was assessed under voltage clamp conditions. ( A ) Representative short-circuit current (I sc ) tracings in wtCFTR + CFBE41o- cells exposed for 72 hours to either continuous (C) or intermittent (I) albuterol or formoterol; aggregate data are presented in B ( n = 4 inserts/condition; circles represent total CFTR activity [cAMP + genistein], diamonds represent inhibited CFTR currents [Inh172]). ( C ) Representative I sc tracings in VX809-treated F508del-CFTR + CFBE41o- cells exposed for 72 hours to either continuous (C) or intermittent (I) albuterol or formoterol; aggregate data are presented in D ( n = 4 inserts/condition). All data were normalized to the control or VX809 (no albuterol pretreatment) condition. Stimulation protocol was as follows: amiloride (100 μM, not shown), cAMP (10 μM forskolin/100 μM IBMX; dark gray bars), CFTR potentiator (50 μM genistein for wtCFTR + , 1 μM VX770 for F508del-CFTR + cells; light gray bars), and CFTR inhibition (10 μM Inh172; white bars). All experiments are representative of studies repeated in duplicate or triplicate with similar results. Data presented represent the mean ± SEM. * P
    Figure Legend Snippet: Continuous exposure to albuterol reduces stimulated CFTR activity in a fashion similar to continuous or intermittent dosing with formoterol. wtCFTR + and VX809-corrected F508del-CFTR + CFBE41o- cells were exposed to either continuous or intermittent (1 hour, twice daily) albuterol (10 μM) or the long-acting β2AR-agonist formoterol (LABA; 1 μM) for 72 hours. Cells were mounted in Ussing chambers and CFTR function was assessed under voltage clamp conditions. ( A ) Representative short-circuit current (I sc ) tracings in wtCFTR + CFBE41o- cells exposed for 72 hours to either continuous (C) or intermittent (I) albuterol or formoterol; aggregate data are presented in B ( n = 4 inserts/condition; circles represent total CFTR activity [cAMP + genistein], diamonds represent inhibited CFTR currents [Inh172]). ( C ) Representative I sc tracings in VX809-treated F508del-CFTR + CFBE41o- cells exposed for 72 hours to either continuous (C) or intermittent (I) albuterol or formoterol; aggregate data are presented in D ( n = 4 inserts/condition). All data were normalized to the control or VX809 (no albuterol pretreatment) condition. Stimulation protocol was as follows: amiloride (100 μM, not shown), cAMP (10 μM forskolin/100 μM IBMX; dark gray bars), CFTR potentiator (50 μM genistein for wtCFTR + , 1 μM VX770 for F508del-CFTR + cells; light gray bars), and CFTR inhibition (10 μM Inh172; white bars). All experiments are representative of studies repeated in duplicate or triplicate with similar results. Data presented represent the mean ± SEM. * P

    Techniques Used: Activity Assay, Inhibition

    Chronic albuterol-induced CFTR impairment is rescued with application of exogenous cAMP. wtCFTR + and VX809-corrected F508del-CFTR + CFBE41o- cells were exposed to albuterol and/or VX809 in media for 72 hours, then mounted in Ussing chambers and CFTR function was assessed under voltage clamp conditions. In contrast to previously demonstrated experiments, cell-permeant 8-bromoadenosine cAMP (8-Br cAMP) was used to stimulate CFTR function to bypass cellular mechanisms of cAMP generation. In wtCFTR + cells (representative short-circuit current [I sc ] tracing in A , aggregate data in B ; n = 4 inserts/condition. Circles represent total CFTR activity [cAMP + genistein], diamonds represent inhibited CFTR currents [Inh172]), maximal stimulation with 8-Br cAMP in place of forskolin/IBMX rescues the previously demonstrated albuterol-induced CFTR dysfunction. Stimulation with increasing doses of 8-Br cAMP ( C , n = 3 inserts/data point) revealed a small increase in CFTR function in albuterol-pretreated cells at low stimulation doses. Squares, control conditions; triangles, albuterol pretreated. Similarly, in VX809-corrected F508del-CFTR + cells (representative I sc tracing in D , aggregate data in E ; n = 4 inserts/condition), maximal stimulation with 8-Br cAMP rescues albuterol-induced CFTR dysfunction. Unlike wtCFTR + cells, stimulation with increasing doses of 8-Br cAMP demonstrated no difference in VX809-corrected F508del-CFTR function following albuterol pretreatment at any stimulation dose ( F , n = 3 inserts/data point. All indicators of statistical significance are comparisons with untreated cells; no difference was noted between VX809-corrected cells with or without albuterol). Circles, untreated; squares, VX809 alone; triangles, VX809 + albuterol. All studies are internally normalized as indicated to allow for comparisons. Stimulation protocol was as follows: amiloride (100 μM, not shown), cAMP (100 μM 8-Br cAMP; dark gray bars), CFTR potentiator (50 μM genistein for wtCFTR + ; 1 μM VX770 for F508del-CFTR + cells; light gray bars), and CFTR inhibition (10 μM Inh172; white bars). Both experiments are representative of studies repeated in duplicate with similar results. Data presented represent the mean ± SEM. * P
    Figure Legend Snippet: Chronic albuterol-induced CFTR impairment is rescued with application of exogenous cAMP. wtCFTR + and VX809-corrected F508del-CFTR + CFBE41o- cells were exposed to albuterol and/or VX809 in media for 72 hours, then mounted in Ussing chambers and CFTR function was assessed under voltage clamp conditions. In contrast to previously demonstrated experiments, cell-permeant 8-bromoadenosine cAMP (8-Br cAMP) was used to stimulate CFTR function to bypass cellular mechanisms of cAMP generation. In wtCFTR + cells (representative short-circuit current [I sc ] tracing in A , aggregate data in B ; n = 4 inserts/condition. Circles represent total CFTR activity [cAMP + genistein], diamonds represent inhibited CFTR currents [Inh172]), maximal stimulation with 8-Br cAMP in place of forskolin/IBMX rescues the previously demonstrated albuterol-induced CFTR dysfunction. Stimulation with increasing doses of 8-Br cAMP ( C , n = 3 inserts/data point) revealed a small increase in CFTR function in albuterol-pretreated cells at low stimulation doses. Squares, control conditions; triangles, albuterol pretreated. Similarly, in VX809-corrected F508del-CFTR + cells (representative I sc tracing in D , aggregate data in E ; n = 4 inserts/condition), maximal stimulation with 8-Br cAMP rescues albuterol-induced CFTR dysfunction. Unlike wtCFTR + cells, stimulation with increasing doses of 8-Br cAMP demonstrated no difference in VX809-corrected F508del-CFTR function following albuterol pretreatment at any stimulation dose ( F , n = 3 inserts/data point. All indicators of statistical significance are comparisons with untreated cells; no difference was noted between VX809-corrected cells with or without albuterol). Circles, untreated; squares, VX809 alone; triangles, VX809 + albuterol. All studies are internally normalized as indicated to allow for comparisons. Stimulation protocol was as follows: amiloride (100 μM, not shown), cAMP (100 μM 8-Br cAMP; dark gray bars), CFTR potentiator (50 μM genistein for wtCFTR + ; 1 μM VX770 for F508del-CFTR + cells; light gray bars), and CFTR inhibition (10 μM Inh172; white bars). Both experiments are representative of studies repeated in duplicate with similar results. Data presented represent the mean ± SEM. * P

    Techniques Used: Activity Assay, Inhibition

    Chronic albuterol exposure reduces forskolin/IBMX–stimulated generation of cAMP in wtCFTR + and F508del-CFTR + CFBE41o- cells. wtCFTR + and F508del-CFTR + CFBE41o- cells were pretreated with albuterol (10 μM) for 72 hours, then stimulated to produce cAMP with 10 μM forskolin/100 μM IBMX (10 minutes). cAMP levels were then analyzed by colorimetric ELISA. wtCFTR + ( A , n = 6) and F508del-CFTR + ( B , n = 6) CFBE41o- cells produce minimal cAMP in the absence of either pretreatment or stimulation (open circles). Stimulation with 10 μM forskolin/100 μM IBMX in untreated cells leads to cAMP production (open squares), which is reduced by more than 60% in cells pretreated for 72 hours with albuterol (open triangles). All studies are internally normalized to control conditions to allow for comparisons. Both experiments are representative of studies repeated in duplicate with similar results. Data presented represent the mean ± SEM. n = 6 wells per condition. **** P
    Figure Legend Snippet: Chronic albuterol exposure reduces forskolin/IBMX–stimulated generation of cAMP in wtCFTR + and F508del-CFTR + CFBE41o- cells. wtCFTR + and F508del-CFTR + CFBE41o- cells were pretreated with albuterol (10 μM) for 72 hours, then stimulated to produce cAMP with 10 μM forskolin/100 μM IBMX (10 minutes). cAMP levels were then analyzed by colorimetric ELISA. wtCFTR + ( A , n = 6) and F508del-CFTR + ( B , n = 6) CFBE41o- cells produce minimal cAMP in the absence of either pretreatment or stimulation (open circles). Stimulation with 10 μM forskolin/100 μM IBMX in untreated cells leads to cAMP production (open squares), which is reduced by more than 60% in cells pretreated for 72 hours with albuterol (open triangles). All studies are internally normalized to control conditions to allow for comparisons. Both experiments are representative of studies repeated in duplicate with similar results. Data presented represent the mean ± SEM. n = 6 wells per condition. **** P

    Techniques Used: Enzyme-linked Immunosorbent Assay

    2) Product Images from "Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum"

    Article Title: Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-00281-x

    KEGG pathway analysis. The fungal mycelium was incubated with cAMP and IBMX. Transcriptome sequencing was performed to identify differentially expressed genes between the control and cAMP-treated mycelium. The differentially expressed genes were further mapped to the KEGG database. The major categories of differentially expressed genes are presented.
    Figure Legend Snippet: KEGG pathway analysis. The fungal mycelium was incubated with cAMP and IBMX. Transcriptome sequencing was performed to identify differentially expressed genes between the control and cAMP-treated mycelium. The differentially expressed genes were further mapped to the KEGG database. The major categories of differentially expressed genes are presented.

    Techniques Used: Incubation, Sequencing

    Effect of cAMP on the mRNA expression levels of squalene synthase (SQS) and lanosterol synthase (LS) in Ganoderma lucidum . G. lucidum mycelium was incubated with 40 mM caffeine, 10 mM NaF, or 40 mM cAMP/15 mM IBMX for 12 h. Northern blotting was then performed to detect the expression of the SQS and LS genes. Gels stained with ethidium bromide are shown to indicate the relative total RNA loading.
    Figure Legend Snippet: Effect of cAMP on the mRNA expression levels of squalene synthase (SQS) and lanosterol synthase (LS) in Ganoderma lucidum . G. lucidum mycelium was incubated with 40 mM caffeine, 10 mM NaF, or 40 mM cAMP/15 mM IBMX for 12 h. Northern blotting was then performed to detect the expression of the SQS and LS genes. Gels stained with ethidium bromide are shown to indicate the relative total RNA loading.

    Techniques Used: Expressing, Incubation, Northern Blot, Staining

    Gene Ontology analysis. The fungal mycelium was incubated with cAMP and IBMX. Transcriptome sequencing was performed to identify differentially expressed genes between the control and cAMP-treated mycelium. The differentially expressed genes were further mapped to the gene ontology (GO) database. Three main categories (biological process, cellular component, and molecular function) are presented.
    Figure Legend Snippet: Gene Ontology analysis. The fungal mycelium was incubated with cAMP and IBMX. Transcriptome sequencing was performed to identify differentially expressed genes between the control and cAMP-treated mycelium. The differentially expressed genes were further mapped to the gene ontology (GO) database. Three main categories (biological process, cellular component, and molecular function) are presented.

    Techniques Used: Incubation, Sequencing

    Apoptosis induction in Ganoderma lucidum . Fungal mycelium was incubated with 20 mM NaF, 80 mM caffeine, or 40 mM cAMP/15 mM IBMX for 16 h prior to staining. ( a ) DNA fragmentation was assessed by TUNEL assay. DAPI staining was used to locate the nucleus and assess the nuclear morphology of the fungal cells. The arrows indicate the two nuclei present in each fungal cell. Untreated fungal cells did not show any fluorescence signal in the TUNEL assay. To further confirm the relevance of the nuclear morphology of the fungal cells, mycelium was pretreated with DNase I to induce DNA breaks and then subjected to TUNEL assay and DAPI staining. ( b ) Isolated protoplasts were stained with FITC-conjugated annexin V and propidium iodide (PI).
    Figure Legend Snippet: Apoptosis induction in Ganoderma lucidum . Fungal mycelium was incubated with 20 mM NaF, 80 mM caffeine, or 40 mM cAMP/15 mM IBMX for 16 h prior to staining. ( a ) DNA fragmentation was assessed by TUNEL assay. DAPI staining was used to locate the nucleus and assess the nuclear morphology of the fungal cells. The arrows indicate the two nuclei present in each fungal cell. Untreated fungal cells did not show any fluorescence signal in the TUNEL assay. To further confirm the relevance of the nuclear morphology of the fungal cells, mycelium was pretreated with DNase I to induce DNA breaks and then subjected to TUNEL assay and DAPI staining. ( b ) Isolated protoplasts were stained with FITC-conjugated annexin V and propidium iodide (PI).

    Techniques Used: Incubation, Staining, TUNEL Assay, Fluorescence, Isolation

    Effect of cAMP and IBMX co-treatment on fungal biomass and production of ganoderic acids. Four-day-old fungal mycelium was cultured on potato dextrose agar (PDA) in 5.5-cm diameter petri dishes and then incubated with 40 mM cAMP and 15 mM IBMX in potato dextrose broth (PDB) for 4 days. Dried mycelium was used to measure fungal biomass ( a ). Lanosta-7,9(11), 24-trien-3α-o1-26-oic acid (ganoderic acid 24) ( b ), and total ganoderic acids (GAs) ( c ) were measured by HPLC. The means of three independent samples with standard deviations are presented. * p
    Figure Legend Snippet: Effect of cAMP and IBMX co-treatment on fungal biomass and production of ganoderic acids. Four-day-old fungal mycelium was cultured on potato dextrose agar (PDA) in 5.5-cm diameter petri dishes and then incubated with 40 mM cAMP and 15 mM IBMX in potato dextrose broth (PDB) for 4 days. Dried mycelium was used to measure fungal biomass ( a ). Lanosta-7,9(11), 24-trien-3α-o1-26-oic acid (ganoderic acid 24) ( b ), and total ganoderic acids (GAs) ( c ) were measured by HPLC. The means of three independent samples with standard deviations are presented. * p

    Techniques Used: Cell Culture, Incubation, High Performance Liquid Chromatography

    3) Product Images from "The identification of inhibitors of Schistosoma mansoni miracidial transformation by incorporating a medium-throughput small-molecule screen"

    Article Title: The identification of inhibitors of Schistosoma mansoni miracidial transformation by incorporating a medium-throughput small-molecule screen

    Journal: Experimental parasitology

    doi: 10.1016/j.exppara.2009.12.021

    Immunocytochemistry analysis of miracidia following a 4 h treatment with 20 µM of IBMX or DMSO and the corresponding relative fluorescence levels demonstrating a significant difference in median relative fluorescence levels (p
    Figure Legend Snippet: Immunocytochemistry analysis of miracidia following a 4 h treatment with 20 µM of IBMX or DMSO and the corresponding relative fluorescence levels demonstrating a significant difference in median relative fluorescence levels (p

    Techniques Used: Immunocytochemistry, Fluorescence

    Graphs showing the transformation dose-responses of Schistosoma mansoni miracidia at 18 h post-treated with (A) citalopram (B) clomipramine (C) triflupromazine (D) PMA (E) IBMX (12 h).
    Figure Legend Snippet: Graphs showing the transformation dose-responses of Schistosoma mansoni miracidia at 18 h post-treated with (A) citalopram (B) clomipramine (C) triflupromazine (D) PMA (E) IBMX (12 h).

    Techniques Used: Transformation Assay

    Photomicrographs of live Schistosoma mansoni miracidia after 4-h treatment with 10 µM (a) citalopram (b) clomipramine (c) triflupromazine (d) forskolin (e) PMA, (f) IBMX or (g) DMSO only (control). Note abnormal appearances in PMA treated parasites
    Figure Legend Snippet: Photomicrographs of live Schistosoma mansoni miracidia after 4-h treatment with 10 µM (a) citalopram (b) clomipramine (c) triflupromazine (d) forskolin (e) PMA, (f) IBMX or (g) DMSO only (control). Note abnormal appearances in PMA treated parasites

    Techniques Used:

    4) Product Images from "Antiadipogenic Effects of Mixtures of Cornus officinalis and Ribes fasciculatum Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice"

    Article Title: Antiadipogenic Effects of Mixtures of Cornus officinalis and Ribes fasciculatum Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice

    Journal: Molecules

    doi: 10.3390/molecules25102350

    Anti-adipogenic effects of Cornus officinalis (CO) and Ribes fasciculatum (RF) on mRNA expression of adipogenic markers in 3T3-L1 cells. Cells were induced by 3-isobutyl-1-methylxanthine, dexamethasone and insulin multiple daily injections and co-incubated with CO (A) and RF (B) at 2, 10, or 50 μg/mL during lipid accumulation. mRNA of adipogenesis-associated genes was analyzed by qRT-PCR. Relative mRNA of Cebpa , Fabp4 , Pparg and Srebp1 was normalized by Gapdh . * p
    Figure Legend Snippet: Anti-adipogenic effects of Cornus officinalis (CO) and Ribes fasciculatum (RF) on mRNA expression of adipogenic markers in 3T3-L1 cells. Cells were induced by 3-isobutyl-1-methylxanthine, dexamethasone and insulin multiple daily injections and co-incubated with CO (A) and RF (B) at 2, 10, or 50 μg/mL during lipid accumulation. mRNA of adipogenesis-associated genes was analyzed by qRT-PCR. Relative mRNA of Cebpa , Fabp4 , Pparg and Srebp1 was normalized by Gapdh . * p

    Techniques Used: Expressing, Incubation, Quantitative RT-PCR

    Related Articles

    Sequencing:

    Article Title: Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum
    Article Snippet: .. RNA extraction and transcriptome sequencing Fungal mycelium was treated with 40 mM cAMP and 15 mM IBMX for 12 h. Total RNA was extracted with TRIzol reagent using the standard protocol (Invitrogen, CA, USA) and DNase was used to remove potential DNA contamination. .. RNA purity and integrity were analyzed using the RNA Nano 6000 Assay Kit with the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

    Concentration Assay:

    Article Title: Discovery of Mixed Pharmacology Melanocortin-3 Agonists and Melanocortin-4 Receptor Tetrapeptide Antagonist Compounds (TACOs) Based on the Sequence Ac-Xaa1-Arg-(pI)DPhe-Xaa4-NH2
    Article Snippet: .. The cell pellet was then re-suspended in a solution of freshly made stimulation buffer [Hank's Balanced Salt Solution (HBSS 10 × without NaHCO3 or phenol red, Gibco), 0.5 mM isobutylmethylxanthine (IBMX), 5 mM HEPES buffer solution (1 M, Gibco), 0.1% BSA in Milli-Q water, pH=7.4] to a final concentration of 10,000 cells/μL. .. A solution of cells and anti-cAMP acceptor beads in stimulation buffer was then prepared (1,000 cells/μL and 0.5 μg/μL AlphaScreen anti-cAMP acceptor beads in stimulation buffer).

    RNA Extraction:

    Article Title: Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum
    Article Snippet: .. RNA extraction and transcriptome sequencing Fungal mycelium was treated with 40 mM cAMP and 15 mM IBMX for 12 h. Total RNA was extracted with TRIzol reagent using the standard protocol (Invitrogen, CA, USA) and DNase was used to remove potential DNA contamination. .. RNA purity and integrity were analyzed using the RNA Nano 6000 Assay Kit with the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

    other:

    Article Title: Skin-Derived Precursors against UVB-Induced Apoptosis via Bcl-2 and Nrf2 Upregulation
    Article Snippet: Adipogenesis inducer solution was basal medium containing 2% 3-isobutyl-1-methylxanthine (IBMX) solution, 1% insulin solution, and 1% dexamethasone solution (Invitrogen, Carlsbad, CA, USA).

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  • 86
    Thermo Fisher forskolin ibmx
    STXBP1 is translocated to the mGLUTag L-cell membrane upon stimulation of GLP-1 secretion. Eight hours after synchronization, mGLUTag L-cells were treated with 50 µM <t>forskolin</t> (F) plus 50 µM <t>IBMX</t> (I) for up to 60 minutes and then immunostained for STXBP1; Wheat Germ Agglutinin was used for the membrane marker. (A) Representative images, (B) STXBP1 cytoplasmic to nuclear intensity ratio (n = 7–9) and (C) Pearson correlation coefficient for colocalization of STXBP1 with the membrane marker (n = 4). * P
    Forskolin Ibmx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/forskolin ibmx/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    forskolin ibmx - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    95
    Thermo Fisher ibmx
    Anti-adipogenic effects of Cornus officinalis (CO) and Ribes fasciculatum (RF) on mRNA expression of adipogenic markers in <t>3T3-L1</t> cells. Cells were induced by <t>3-isobutyl-1-methylxanthine,</t> dexamethasone and insulin multiple daily injections and co-incubated with CO (A) and RF (B) at 2, 10, or 50 μg/mL during lipid accumulation. mRNA of adipogenesis-associated genes was analyzed by qRT-PCR. Relative mRNA of Cebpa , Fabp4 , Pparg and Srebp1 was normalized by Gapdh . * p
    Ibmx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ibmx/product/Thermo Fisher
    Average 95 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    ibmx - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    STXBP1 is translocated to the mGLUTag L-cell membrane upon stimulation of GLP-1 secretion. Eight hours after synchronization, mGLUTag L-cells were treated with 50 µM forskolin (F) plus 50 µM IBMX (I) for up to 60 minutes and then immunostained for STXBP1; Wheat Germ Agglutinin was used for the membrane marker. (A) Representative images, (B) STXBP1 cytoplasmic to nuclear intensity ratio (n = 7–9) and (C) Pearson correlation coefficient for colocalization of STXBP1 with the membrane marker (n = 4). * P

    Journal: Endocrinology

    Article Title: Essential Role of Syntaxin-Binding Protein-1 in the Regulation of Glucagon-Like Peptide-1 Secretion

    doi: 10.1210/endocr/bqaa039

    Figure Lengend Snippet: STXBP1 is translocated to the mGLUTag L-cell membrane upon stimulation of GLP-1 secretion. Eight hours after synchronization, mGLUTag L-cells were treated with 50 µM forskolin (F) plus 50 µM IBMX (I) for up to 60 minutes and then immunostained for STXBP1; Wheat Germ Agglutinin was used for the membrane marker. (A) Representative images, (B) STXBP1 cytoplasmic to nuclear intensity ratio (n = 7–9) and (C) Pearson correlation coefficient for colocalization of STXBP1 with the membrane marker (n = 4). * P

    Article Snippet: After treatment for up to 60 minutes with forskolin/IBMX, the plasma membrane of live mGLUTag L-cells was labelled with 2.5 µg/μL Wheat Germ Agglutinin-Alexa Fluor 488 Conjugate (ThermoFisher Scientific) in Hanks’ balanced salt solution at 37°C for 10 minutes.

    Techniques: Marker

    KO of Stxbp1 in the primary L-cell impairs GLP-1 secretion ex vivo. GLP-1 secretion assay in primary adult mouse ileal crypt cultures from L-cell Stxbp1 KO and control mice. Cells were treated for 2 hours with 50 µM forskolin (F) plus 50 µM IBMX (I) or vehicle alone (control). (A) Percent GLP-1 secretion and (B) total cell content of GLP-1. (n = 6–7). * P

    Journal: Endocrinology

    Article Title: Essential Role of Syntaxin-Binding Protein-1 in the Regulation of Glucagon-Like Peptide-1 Secretion

    doi: 10.1210/endocr/bqaa039

    Figure Lengend Snippet: KO of Stxbp1 in the primary L-cell impairs GLP-1 secretion ex vivo. GLP-1 secretion assay in primary adult mouse ileal crypt cultures from L-cell Stxbp1 KO and control mice. Cells were treated for 2 hours with 50 µM forskolin (F) plus 50 µM IBMX (I) or vehicle alone (control). (A) Percent GLP-1 secretion and (B) total cell content of GLP-1. (n = 6–7). * P

    Article Snippet: After treatment for up to 60 minutes with forskolin/IBMX, the plasma membrane of live mGLUTag L-cells was labelled with 2.5 µg/μL Wheat Germ Agglutinin-Alexa Fluor 488 Conjugate (ThermoFisher Scientific) in Hanks’ balanced salt solution at 37°C for 10 minutes.

    Techniques: Ex Vivo, Mouse Assay

    Anti-adipogenic effects of Cornus officinalis (CO) and Ribes fasciculatum (RF) on mRNA expression of adipogenic markers in 3T3-L1 cells. Cells were induced by 3-isobutyl-1-methylxanthine, dexamethasone and insulin multiple daily injections and co-incubated with CO (A) and RF (B) at 2, 10, or 50 μg/mL during lipid accumulation. mRNA of adipogenesis-associated genes was analyzed by qRT-PCR. Relative mRNA of Cebpa , Fabp4 , Pparg and Srebp1 was normalized by Gapdh . * p

    Journal: Molecules

    Article Title: Antiadipogenic Effects of Mixtures of Cornus officinalis and Ribes fasciculatum Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice

    doi: 10.3390/molecules25102350

    Figure Lengend Snippet: Anti-adipogenic effects of Cornus officinalis (CO) and Ribes fasciculatum (RF) on mRNA expression of adipogenic markers in 3T3-L1 cells. Cells were induced by 3-isobutyl-1-methylxanthine, dexamethasone and insulin multiple daily injections and co-incubated with CO (A) and RF (B) at 2, 10, or 50 μg/mL during lipid accumulation. mRNA of adipogenesis-associated genes was analyzed by qRT-PCR. Relative mRNA of Cebpa , Fabp4 , Pparg and Srebp1 was normalized by Gapdh . * p

    Article Snippet: For adipocyte differentiation, pre-adipocyte 3T3-L1 cells were incubated with DMEM containing 0.5 mM 3-isobutyl-1-methylxanthine, 1 mM dexamethasone and 1 μg/mL insulin.

    Techniques: Expressing, Incubation, Quantitative RT-PCR