2 c methyl d erythritol 2  (Echelon Biosciences)


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    Structured Review

    Echelon Biosciences 2 c methyl d erythritol 2
    Quantitative proteomics comparison of Seq + and MG1655* during lycopene production. a Selective Reaction Monitoring (SRM) proteomics for all MEP and lycopene production pathway enzymes measured in this study. b Lycopene biosynthesis pathway in E. coli . Shown are all enzymes involved in the formation of lycopene from glyceraldehyde-3-phosphate (GA3P) and pyruvate (PYR), as well as reactions competing for either isopentenyl pyrophosphate (IPP), dimethylallyl-pyrophosphate (DMAPP) or farnesyl-pyrophosphate (FPP). Peptides for MiaA and IspU were obtained but did not match our quality criteria and were excluded from this study. c Gene Set Enrichment Analysis of differentially expressed genes in the lycopene production phase. Up- or down-regulated proteins are shown for the Δ rpoS strain MG1655* relative to Seq + . Abbreviations: MEP pathway: Dxs, 1-deoxyxylulose-5-phosphate synthase; Dxr, 1-deoxy- d -xylulose-5-phosphate reductoisomerasease; IspD, <t>4-diphosphocytidyl-2-C-methyl-</t> d -erythritol synthase; IspE, 4-diphosphocytidyl-2-C-methyl- d -erythritol kinase; IspF, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate synthase; IspG, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; IspH, hydroxymethylbutenyl diphosphate reductase; Idi, isopentenyl diphosphate isomerase. Competing pathways: IspA, farnesyl pyrophosphate synthase; IspB, octaprenyl diphosphate synthase; IspU, undecaprenyl diphosphate synthase; MiaA, tRNA(i 6 A37) synthase. pAC-LYC04: Idi(pLYC) from H. pluvialis ; CrtE, geranylgeranyl diphosphate synthase from P. agglomerans ; CrtB, phytoene synthase from P. agglomerans ; CrtI, phytoene desaturase from P. agglomerans
    2 C Methyl D Erythritol 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 c methyl d erythritol 2/product/Echelon Biosciences
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 c methyl d erythritol 2 - by Bioz Stars, 2022-12
    85/100 stars

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    1) Product Images from "Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite"

    Article Title: Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-015-0381-7

    Quantitative proteomics comparison of Seq + and MG1655* during lycopene production. a Selective Reaction Monitoring (SRM) proteomics for all MEP and lycopene production pathway enzymes measured in this study. b Lycopene biosynthesis pathway in E. coli . Shown are all enzymes involved in the formation of lycopene from glyceraldehyde-3-phosphate (GA3P) and pyruvate (PYR), as well as reactions competing for either isopentenyl pyrophosphate (IPP), dimethylallyl-pyrophosphate (DMAPP) or farnesyl-pyrophosphate (FPP). Peptides for MiaA and IspU were obtained but did not match our quality criteria and were excluded from this study. c Gene Set Enrichment Analysis of differentially expressed genes in the lycopene production phase. Up- or down-regulated proteins are shown for the Δ rpoS strain MG1655* relative to Seq + . Abbreviations: MEP pathway: Dxs, 1-deoxyxylulose-5-phosphate synthase; Dxr, 1-deoxy- d -xylulose-5-phosphate reductoisomerasease; IspD, 4-diphosphocytidyl-2-C-methyl- d -erythritol synthase; IspE, 4-diphosphocytidyl-2-C-methyl- d -erythritol kinase; IspF, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate synthase; IspG, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; IspH, hydroxymethylbutenyl diphosphate reductase; Idi, isopentenyl diphosphate isomerase. Competing pathways: IspA, farnesyl pyrophosphate synthase; IspB, octaprenyl diphosphate synthase; IspU, undecaprenyl diphosphate synthase; MiaA, tRNA(i 6 A37) synthase. pAC-LYC04: Idi(pLYC) from H. pluvialis ; CrtE, geranylgeranyl diphosphate synthase from P. agglomerans ; CrtB, phytoene synthase from P. agglomerans ; CrtI, phytoene desaturase from P. agglomerans
    Figure Legend Snippet: Quantitative proteomics comparison of Seq + and MG1655* during lycopene production. a Selective Reaction Monitoring (SRM) proteomics for all MEP and lycopene production pathway enzymes measured in this study. b Lycopene biosynthesis pathway in E. coli . Shown are all enzymes involved in the formation of lycopene from glyceraldehyde-3-phosphate (GA3P) and pyruvate (PYR), as well as reactions competing for either isopentenyl pyrophosphate (IPP), dimethylallyl-pyrophosphate (DMAPP) or farnesyl-pyrophosphate (FPP). Peptides for MiaA and IspU were obtained but did not match our quality criteria and were excluded from this study. c Gene Set Enrichment Analysis of differentially expressed genes in the lycopene production phase. Up- or down-regulated proteins are shown for the Δ rpoS strain MG1655* relative to Seq + . Abbreviations: MEP pathway: Dxs, 1-deoxyxylulose-5-phosphate synthase; Dxr, 1-deoxy- d -xylulose-5-phosphate reductoisomerasease; IspD, 4-diphosphocytidyl-2-C-methyl- d -erythritol synthase; IspE, 4-diphosphocytidyl-2-C-methyl- d -erythritol kinase; IspF, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate synthase; IspG, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; IspH, hydroxymethylbutenyl diphosphate reductase; Idi, isopentenyl diphosphate isomerase. Competing pathways: IspA, farnesyl pyrophosphate synthase; IspB, octaprenyl diphosphate synthase; IspU, undecaprenyl diphosphate synthase; MiaA, tRNA(i 6 A37) synthase. pAC-LYC04: Idi(pLYC) from H. pluvialis ; CrtE, geranylgeranyl diphosphate synthase from P. agglomerans ; CrtB, phytoene synthase from P. agglomerans ; CrtI, phytoene desaturase from P. agglomerans

    Techniques Used:

    MEP pathway flux is not affected by RpoS. a Intracellular MEP pathway metabolite pools during lycopene production in MG1655* and Seq + with dxs overexpression. Glyceraldehyde-3phosphate (GA3P), dihydroxyacetone-phosphate (DHAP), pyruvate (PYR), 1-deoxy- d -xylulose 5-phosphate (DXP), 2-C-methyl- d -erythritol-4-phosphate (MEP), 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME), 2-C-methyl- d -erythritol-2,4-cyclodiphosphate (MEcPP), (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), isopentenyl pyrophosphate (IPP)/dimethylallyl pyrophosphate (DMAPP; no chromatographic separation of IPP and DMAPP) and farnesyl pyrophosphate (FPP) were quantified using liquid chromatography tandem mass spectrometry (LC–MS/MS). 4-Diphosphocytidyl-2-C-methyl- d -erythritol-2-phosphate (CDP-MEP) was below detection level in all samples. b Isoprene production in the two strains harboring pT-ispS(L70R)
    Figure Legend Snippet: MEP pathway flux is not affected by RpoS. a Intracellular MEP pathway metabolite pools during lycopene production in MG1655* and Seq + with dxs overexpression. Glyceraldehyde-3phosphate (GA3P), dihydroxyacetone-phosphate (DHAP), pyruvate (PYR), 1-deoxy- d -xylulose 5-phosphate (DXP), 2-C-methyl- d -erythritol-4-phosphate (MEP), 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME), 2-C-methyl- d -erythritol-2,4-cyclodiphosphate (MEcPP), (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), isopentenyl pyrophosphate (IPP)/dimethylallyl pyrophosphate (DMAPP; no chromatographic separation of IPP and DMAPP) and farnesyl pyrophosphate (FPP) were quantified using liquid chromatography tandem mass spectrometry (LC–MS/MS). 4-Diphosphocytidyl-2-C-methyl- d -erythritol-2-phosphate (CDP-MEP) was below detection level in all samples. b Isoprene production in the two strains harboring pT-ispS(L70R)

    Techniques Used: Over Expression, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

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    Echelon Biosciences 2 c methyl d erythritol 2
    Quantitative proteomics comparison of Seq + and MG1655* during lycopene production. a Selective Reaction Monitoring (SRM) proteomics for all MEP and lycopene production pathway enzymes measured in this study. b Lycopene biosynthesis pathway in E. coli . Shown are all enzymes involved in the formation of lycopene from glyceraldehyde-3-phosphate (GA3P) and pyruvate (PYR), as well as reactions competing for either isopentenyl pyrophosphate (IPP), dimethylallyl-pyrophosphate (DMAPP) or farnesyl-pyrophosphate (FPP). Peptides for MiaA and IspU were obtained but did not match our quality criteria and were excluded from this study. c Gene Set Enrichment Analysis of differentially expressed genes in the lycopene production phase. Up- or down-regulated proteins are shown for the Δ rpoS strain MG1655* relative to Seq + . Abbreviations: MEP pathway: Dxs, 1-deoxyxylulose-5-phosphate synthase; Dxr, 1-deoxy- d -xylulose-5-phosphate reductoisomerasease; IspD, <t>4-diphosphocytidyl-2-C-methyl-</t> d -erythritol synthase; IspE, 4-diphosphocytidyl-2-C-methyl- d -erythritol kinase; IspF, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate synthase; IspG, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; IspH, hydroxymethylbutenyl diphosphate reductase; Idi, isopentenyl diphosphate isomerase. Competing pathways: IspA, farnesyl pyrophosphate synthase; IspB, octaprenyl diphosphate synthase; IspU, undecaprenyl diphosphate synthase; MiaA, tRNA(i 6 A37) synthase. pAC-LYC04: Idi(pLYC) from H. pluvialis ; CrtE, geranylgeranyl diphosphate synthase from P. agglomerans ; CrtB, phytoene synthase from P. agglomerans ; CrtI, phytoene desaturase from P. agglomerans
    2 C Methyl D Erythritol 2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 c methyl d erythritol 2/product/Echelon Biosciences
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 c methyl d erythritol 2 - by Bioz Stars, 2022-12
    85/100 stars
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    Quantitative proteomics comparison of Seq + and MG1655* during lycopene production. a Selective Reaction Monitoring (SRM) proteomics for all MEP and lycopene production pathway enzymes measured in this study. b Lycopene biosynthesis pathway in E. coli . Shown are all enzymes involved in the formation of lycopene from glyceraldehyde-3-phosphate (GA3P) and pyruvate (PYR), as well as reactions competing for either isopentenyl pyrophosphate (IPP), dimethylallyl-pyrophosphate (DMAPP) or farnesyl-pyrophosphate (FPP). Peptides for MiaA and IspU were obtained but did not match our quality criteria and were excluded from this study. c Gene Set Enrichment Analysis of differentially expressed genes in the lycopene production phase. Up- or down-regulated proteins are shown for the Δ rpoS strain MG1655* relative to Seq + . Abbreviations: MEP pathway: Dxs, 1-deoxyxylulose-5-phosphate synthase; Dxr, 1-deoxy- d -xylulose-5-phosphate reductoisomerasease; IspD, 4-diphosphocytidyl-2-C-methyl- d -erythritol synthase; IspE, 4-diphosphocytidyl-2-C-methyl- d -erythritol kinase; IspF, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate synthase; IspG, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; IspH, hydroxymethylbutenyl diphosphate reductase; Idi, isopentenyl diphosphate isomerase. Competing pathways: IspA, farnesyl pyrophosphate synthase; IspB, octaprenyl diphosphate synthase; IspU, undecaprenyl diphosphate synthase; MiaA, tRNA(i 6 A37) synthase. pAC-LYC04: Idi(pLYC) from H. pluvialis ; CrtE, geranylgeranyl diphosphate synthase from P. agglomerans ; CrtB, phytoene synthase from P. agglomerans ; CrtI, phytoene desaturase from P. agglomerans

    Journal: Microbial Cell Factories

    Article Title: Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite

    doi: 10.1186/s12934-015-0381-7

    Figure Lengend Snippet: Quantitative proteomics comparison of Seq + and MG1655* during lycopene production. a Selective Reaction Monitoring (SRM) proteomics for all MEP and lycopene production pathway enzymes measured in this study. b Lycopene biosynthesis pathway in E. coli . Shown are all enzymes involved in the formation of lycopene from glyceraldehyde-3-phosphate (GA3P) and pyruvate (PYR), as well as reactions competing for either isopentenyl pyrophosphate (IPP), dimethylallyl-pyrophosphate (DMAPP) or farnesyl-pyrophosphate (FPP). Peptides for MiaA and IspU were obtained but did not match our quality criteria and were excluded from this study. c Gene Set Enrichment Analysis of differentially expressed genes in the lycopene production phase. Up- or down-regulated proteins are shown for the Δ rpoS strain MG1655* relative to Seq + . Abbreviations: MEP pathway: Dxs, 1-deoxyxylulose-5-phosphate synthase; Dxr, 1-deoxy- d -xylulose-5-phosphate reductoisomerasease; IspD, 4-diphosphocytidyl-2-C-methyl- d -erythritol synthase; IspE, 4-diphosphocytidyl-2-C-methyl- d -erythritol kinase; IspF, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate synthase; IspG, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; IspH, hydroxymethylbutenyl diphosphate reductase; Idi, isopentenyl diphosphate isomerase. Competing pathways: IspA, farnesyl pyrophosphate synthase; IspB, octaprenyl diphosphate synthase; IspU, undecaprenyl diphosphate synthase; MiaA, tRNA(i 6 A37) synthase. pAC-LYC04: Idi(pLYC) from H. pluvialis ; CrtE, geranylgeranyl diphosphate synthase from P. agglomerans ; CrtB, phytoene synthase from P. agglomerans ; CrtI, phytoene desaturase from P. agglomerans

    Article Snippet: 1-Deoxy-d -xylulose 5-phosphate (DXP), 2-C-methyl-d -erythritol-4-phosphate (MEP), 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME), 4-diphosphocytidyl-2-C-methyl-d -erythritol 2-phosphate (CDP-MEP, synthesis kit), 2-C-methyl-d-erythritol-2,4-cyclodiphosphate (MEcPP), (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP) and farnesyl pyrophosphate (FPP) were purchased from Echelon Biosciences (Salt Lake City, UT, USA).

    Techniques:

    MEP pathway flux is not affected by RpoS. a Intracellular MEP pathway metabolite pools during lycopene production in MG1655* and Seq + with dxs overexpression. Glyceraldehyde-3phosphate (GA3P), dihydroxyacetone-phosphate (DHAP), pyruvate (PYR), 1-deoxy- d -xylulose 5-phosphate (DXP), 2-C-methyl- d -erythritol-4-phosphate (MEP), 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME), 2-C-methyl- d -erythritol-2,4-cyclodiphosphate (MEcPP), (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), isopentenyl pyrophosphate (IPP)/dimethylallyl pyrophosphate (DMAPP; no chromatographic separation of IPP and DMAPP) and farnesyl pyrophosphate (FPP) were quantified using liquid chromatography tandem mass spectrometry (LC–MS/MS). 4-Diphosphocytidyl-2-C-methyl- d -erythritol-2-phosphate (CDP-MEP) was below detection level in all samples. b Isoprene production in the two strains harboring pT-ispS(L70R)

    Journal: Microbial Cell Factories

    Article Title: Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite

    doi: 10.1186/s12934-015-0381-7

    Figure Lengend Snippet: MEP pathway flux is not affected by RpoS. a Intracellular MEP pathway metabolite pools during lycopene production in MG1655* and Seq + with dxs overexpression. Glyceraldehyde-3phosphate (GA3P), dihydroxyacetone-phosphate (DHAP), pyruvate (PYR), 1-deoxy- d -xylulose 5-phosphate (DXP), 2-C-methyl- d -erythritol-4-phosphate (MEP), 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME), 2-C-methyl- d -erythritol-2,4-cyclodiphosphate (MEcPP), (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), isopentenyl pyrophosphate (IPP)/dimethylallyl pyrophosphate (DMAPP; no chromatographic separation of IPP and DMAPP) and farnesyl pyrophosphate (FPP) were quantified using liquid chromatography tandem mass spectrometry (LC–MS/MS). 4-Diphosphocytidyl-2-C-methyl- d -erythritol-2-phosphate (CDP-MEP) was below detection level in all samples. b Isoprene production in the two strains harboring pT-ispS(L70R)

    Article Snippet: 1-Deoxy-d -xylulose 5-phosphate (DXP), 2-C-methyl-d -erythritol-4-phosphate (MEP), 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME), 4-diphosphocytidyl-2-C-methyl-d -erythritol 2-phosphate (CDP-MEP, synthesis kit), 2-C-methyl-d-erythritol-2,4-cyclodiphosphate (MEcPP), (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP) and farnesyl pyrophosphate (FPP) were purchased from Echelon Biosciences (Salt Lake City, UT, USA).

    Techniques: Over Expression, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy