iκb kinase  (Abcam)

 
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    Name:
    Anti IKK alpha IKK beta EPR16628 antibody
    Description:

    Catalog Number:
    ab178870
    Price:
    None
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    Structured Review

    Abcam iκb kinase

    https://www.bioz.com/result/iκb kinase/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iκb kinase - by Bioz Stars, 2020-09
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: Tanshinone-IIA attenuates the deleterious effects of oxidative stress in osteoporosis through the NF-κB signaling pathway
    Article Snippet: .. For the western blot analysis, primary antibodies: p65 (1:1,000, ab16502; Abcam, Cambridge, UK), IKK-β (1:1,000, ab178870; Abcam), IκBα (1:1,000, ab109300; Abcam) and β-actin (1:1,000, ab8227; Abcam) were added following blocking (5% skimmed milk) for 1 h at 37°C, following which they were incubated with horseradish peroxidase-conjugated immunoglobulin G (cat. no. STAR206P; Bio-Rad Laboratories, Inc.) was used at a 1:5,000 dilution for 24 h at 4°C. ..

    Incubation:

    Article Title: The short-term effect of histone deacetylase inhibitors, chidamide and valproic acid, on the NF-κB pathway in multiple myeloma cells
    Article Snippet: .. The membranes were then incubated with the following primary antibodies at 4°C overnight: Anti-IκBα (1:1,000; cat. no ab32518; Abcam, Cambridge, MA, USA), anti-NF-κB p65 (1:50,000; cat. no. ab32536; Abcam), anti-IKKα/β (1:1,000; cat. no. ab178870; Abcam), anti- phosphorylated (p)-IKKα/β (1:1,000; cat. no. 2697; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-acetyl-histone H3 (1:1,000; cat. no. 9649; Cell Signaling Technology, Inc.) and anti-GAPDH (1:1,000; cat. no. ab9485; Abcam) as an internal control. .. Subsequently, membranes were visualized using an enhanced chemiluminescence (ECL) plus reagent (Western Lightning Plus-ECL; cat. no. NEL104001EA; PerkinElmer, Inc., Waltham, MA, USA), following the addition of a horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h (1:2,500; cat. no. TA140002; OriGene Technologies, Inc., Beijing, China), under a chemiluminescence and fluorescence imaging system (LumiX® /ChemiX® /FluorChemiX® ; Federal Bioproducts, Inc., London, United Kingdom).

    Article Title: lncRNA‐ NKILA/ NF‐κB feedback loop modulates laryngeal cancer cell proliferation, invasion, and radioresistance
    Article Snippet: .. The blots were probed with the following antibodies: anti‐p65 (ab16502; Abcam, Cambridge, MA), anti‐IKK (ab178870; Abcam), anti‐p‐IKKβ (phospho Y199; ab59195; Abcam), anti‐Iκ Bα (#9242; Cell Signaling Technology, Danvers, MA), anti‐p‐Iκ Bα (Ser 32; sc‐7977; Santa Cruz, Dallas, TX), anti‐β ‐actin (ab6276; Abcam), and anti‐Lamin B (ab133741; Abcam) at 4°C overnight, subsequently incubated with HRP‐conjugated secondary antibody (1:5000). .. ECL substrates were used to visualize signals (Millipore, MA). β ‐actin was used as an endogenous protein for normalization.

    Article Title: lncRNA‐ NKILA/ NF‐κB feedback loop modulates laryngeal cancer cell proliferation, invasion, and radioresistance
    Article Snippet: .. The blots were probed with the following antibodies: anti‐p65 (ab16502; Abcam, Cambridge, MA), anti‐IKK (ab178870; Abcam), anti‐p‐IKK β (phospho Y199; ab59195; Abcam), anti‐I κ B α (#9242; Cell Signaling Technology, Danvers, MA), anti‐p‐I κ B α (Ser 32; sc‐7977; Santa Cruz, Dallas, TX), anti‐ β ‐actin (ab6276; Abcam), and anti‐Lamin B (ab133741; Abcam) at 4°C overnight, subsequently incubated with HRP‐conjugated secondary antibody (1:5000). .. ECL substrates were used to visualize signals (Millipore, MA). β ‐actin was used as an endogenous protein for normalization.

    Article Title: Tanshinone-IIA attenuates the deleterious effects of oxidative stress in osteoporosis through the NF-κB signaling pathway
    Article Snippet: .. For the western blot analysis, primary antibodies: p65 (1:1,000, ab16502; Abcam, Cambridge, UK), IKK-β (1:1,000, ab178870; Abcam), IκBα (1:1,000, ab109300; Abcam) and β-actin (1:1,000, ab8227; Abcam) were added following blocking (5% skimmed milk) for 1 h at 37°C, following which they were incubated with horseradish peroxidase-conjugated immunoglobulin G (cat. no. STAR206P; Bio-Rad Laboratories, Inc.) was used at a 1:5,000 dilution for 24 h at 4°C. ..

    Blocking Assay:

    Article Title: Tanshinone-IIA attenuates the deleterious effects of oxidative stress in osteoporosis through the NF-κB signaling pathway
    Article Snippet: .. For the western blot analysis, primary antibodies: p65 (1:1,000, ab16502; Abcam, Cambridge, UK), IKK-β (1:1,000, ab178870; Abcam), IκBα (1:1,000, ab109300; Abcam) and β-actin (1:1,000, ab8227; Abcam) were added following blocking (5% skimmed milk) for 1 h at 37°C, following which they were incubated with horseradish peroxidase-conjugated immunoglobulin G (cat. no. STAR206P; Bio-Rad Laboratories, Inc.) was used at a 1:5,000 dilution for 24 h at 4°C. ..

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  • 93
    Abcam selective cell permeable iκb kinase ikk 2
    Proposed mechanism of serum amyloid A 1 (SAA1) induced muscle atrophy. SAA1 binds to muscular Toll‐like receptors (TLR) 2 and 4, which results in an activation of the canonical NF‐κB p65 pathway leading to NF‐κB p65/RelA phosphorylation and its translocation into the nucleus. In the nucleus, NF‐κB p65/RelA binds to NF‐κB response elements (NRE), which leads to increased expression and secretion of the pro‐inflammatory cytokine Il6 . IL6 in turn induces expression and secretion of SAA1 resulting in a positive feedback loop. In addition, SAA1 causes an increase of MuRF1/ Trim63 and Atrogin1/ Fbxo32 , which mediate muscle atrophy in sepsis. Anti‐TLR2 or anti‐TLR4 antibodies inhibit SAA1‐induced Il6 expression. The specific <t>IκB</t> kinase subunit <t>IKK‐2</t> inhibitor BMS‐345541 inhibits SAA1‐induced and NF‐κB dependent gene expression, thereby reducing SAA1‐mediated atrophy and inhibiting the self‐augmenting SAA1‐IL6 feedback loop.
    Selective Cell Permeable Iκb Kinase Ikk 2, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/selective cell permeable iκb kinase ikk 2/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    selective cell permeable iκb kinase ikk 2 - by Bioz Stars, 2020-09
    93/100 stars
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    92
    Abcam iκb kinase ikk β
    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, <t>IKK-β</t> and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P
    Iκb Kinase Ikk β, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iκb kinase ikk β/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    iκb kinase ikk β - by Bioz Stars, 2020-09
    92/100 stars
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    95
    Abcam nf κb iκb kinase kinase ikk β
    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; <t>IKK,</t> <t>IκB</t> kinase β; J, control; co, co-cultured.
    Nf κb Iκb Kinase Kinase Ikk β, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nf κb iκb kinase kinase ikk β/product/Abcam
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nf κb iκb kinase kinase ikk β - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Proposed mechanism of serum amyloid A 1 (SAA1) induced muscle atrophy. SAA1 binds to muscular Toll‐like receptors (TLR) 2 and 4, which results in an activation of the canonical NF‐κB p65 pathway leading to NF‐κB p65/RelA phosphorylation and its translocation into the nucleus. In the nucleus, NF‐κB p65/RelA binds to NF‐κB response elements (NRE), which leads to increased expression and secretion of the pro‐inflammatory cytokine Il6 . IL6 in turn induces expression and secretion of SAA1 resulting in a positive feedback loop. In addition, SAA1 causes an increase of MuRF1/ Trim63 and Atrogin1/ Fbxo32 , which mediate muscle atrophy in sepsis. Anti‐TLR2 or anti‐TLR4 antibodies inhibit SAA1‐induced Il6 expression. The specific IκB kinase subunit IKK‐2 inhibitor BMS‐345541 inhibits SAA1‐induced and NF‐κB dependent gene expression, thereby reducing SAA1‐mediated atrophy and inhibiting the self‐augmenting SAA1‐IL6 feedback loop.

    Journal: Journal of Cachexia, Sarcopenia and Muscle

    Article Title: Serum amyloid A1 mediates myotube atrophy via Toll‐like receptors) Serum amyloid A1 mediates myotube atrophy via Toll‐like receptors

    doi: 10.1002/jcsm.12491

    Figure Lengend Snippet: Proposed mechanism of serum amyloid A 1 (SAA1) induced muscle atrophy. SAA1 binds to muscular Toll‐like receptors (TLR) 2 and 4, which results in an activation of the canonical NF‐κB p65 pathway leading to NF‐κB p65/RelA phosphorylation and its translocation into the nucleus. In the nucleus, NF‐κB p65/RelA binds to NF‐κB response elements (NRE), which leads to increased expression and secretion of the pro‐inflammatory cytokine Il6 . IL6 in turn induces expression and secretion of SAA1 resulting in a positive feedback loop. In addition, SAA1 causes an increase of MuRF1/ Trim63 and Atrogin1/ Fbxo32 , which mediate muscle atrophy in sepsis. Anti‐TLR2 or anti‐TLR4 antibodies inhibit SAA1‐induced Il6 expression. The specific IκB kinase subunit IKK‐2 inhibitor BMS‐345541 inhibits SAA1‐induced and NF‐κB dependent gene expression, thereby reducing SAA1‐mediated atrophy and inhibiting the self‐augmenting SAA1‐IL6 feedback loop.

    Article Snippet: BMS‐345541 (4(2'‐aminoethyl)amino‐1,8‐dimethylimidazo(1,2‐a)quinoxaline; CAS‐number: 547757‐23‐3) is a highly selective cell permeable IκB kinase (IKK) 2 and IKK‐1 inhibitor, which blocks NF‐κB‐dependent transcription; it was produced by BMS and purchased from abcam (ab 144822).

    Techniques: Activation Assay, Translocation Assay, Expressing

    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Journal: Molecular Medicine Reports

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma

    doi: 10.3892/mmr.2018.8945

    Figure Lengend Snippet: Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Article Snippet: For western blotting, primary mouse anti-human antibodies against p65 (ab16502; 1:2,000), PI3K and (ab40776; 1:2,000), AKT (ab8805; 1:1,000), tumor necrosis factor (TNF)-α (ab6671; 1:2,000), IL-2 (cat. no. ab92381; 1:2,000), IκB kinase (IKK)-β (ab7547; 1:2,000), NF-κB inhibitor α (IκBα; ab133478; 1:2,000), pAKT (ab38449; 1:12,000) and β-actin (ab8827; 1:2,000; all from Abcam, Cambridge, UK) were added to the membranes after blocking with 5% skimmed milk for 2 h at 37°C.

    Techniques: Activity Assay, Expressing, Migration

    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Journal: Molecular Medicine Reports

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma

    doi: 10.3892/mmr.2018.8945

    Figure Lengend Snippet: Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Article Snippet: For western blotting, primary mouse anti-human antibodies against p65 (ab16502; 1:2,000), PI3K and (ab40776; 1:2,000), AKT (ab8805; 1:1,000), tumor necrosis factor (TNF)-α (ab6671; 1:2,000), IL-2 (cat. no. ab92381; 1:2,000), IκB kinase (IKK)-β (ab7547; 1:2,000), NF-κB inhibitor α (IκBα; ab133478; 1:2,000), pAKT (ab38449; 1:12,000) and β-actin (ab8827; 1:2,000; all from Abcam, Cambridge, UK) were added to the membranes after blocking with 5% skimmed milk for 2 h at 37°C.

    Techniques: Activity Assay, Expressing, Migration

    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; IKK, IκB kinase β; J, control; co, co-cultured.

    Journal: International Journal of Molecular Medicine

    Article Title: Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK

    doi: 10.3892/ijmm.2018.3953

    Figure Lengend Snippet: Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; IKK, IκB kinase β; J, control; co, co-cultured.

    Article Snippet: The membranes were probed with primary antibodies against phosphorylated (p)-P65 (1:2,000, cat. no. AF2006, Affinity Biosciences, Cambridge, UK), P65 (1:2,000, cat. no. 10745-1-AP, ProteinTech Group, Inc., Wuhan, China), inhibitor of NF-κB (IκB) kinase (IKK)β (1:1,000, cat. no. Ab124957, Abcam, Cambridge, MA, USA), IκBα (1:1,000, cat. no. 4812, Cell Signaling Technology, Inc., Danvers, MA, USA), B-cell lymphoma 2 (Bcl-2; 1:2,000, cat. no. 12789-1-AP, ProteinTech Group, Inc.), Bcl-2-associated X protein (Bax; 1:5,000, cat. no. 50599-2-IG, Protein Tech Group, Inc), JNK1/2 (1:1,000, cat. no. 9252, Cell Signaling Technology, Inc.), p-JNK1/2 (1:1,000, cat. no. 4668, Cell Signaling Technology, Inc.), extracellular signal-regulated kinase (ERK)1/2 (1:1,000, cat. no. 9102, Cell Signaling Technology, Inc.), p-ERK1/2 (1:2,000, cat. no. 4370, Cell Signaling Technology, Inc.), P38 (1:1,000, cat. no. 9212, Cell Signaling, Technology, Inc.), p-P38 (1:1,000, cat. no. 9211, Cell Signaling Technology, Inc.), mothers against decapentaplegic (Smad)2/3 (1:1,000, cat. no. AF6367, Affinity Biosciences), p-Smad2/3 (1:200, cat. no. MAB8935, R & D Systems, Inc.) and the loading control β-actin (1:200, cat. no. BM0627, Boster Biological Technology, Wuhan, China).

    Techniques: Cell Culture, Western Blot, Expressing