iκb kinase ikk β  (Abcam)

 
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    Name:
    Anti IKB beta antibody
    Description:

    Catalog Number:
    ab7547
    Price:
    None
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    Structured Review

    Abcam iκb kinase ikk β
    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, <t>IKK-β</t> and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    https://www.bioz.com/result/iκb kinase ikk β/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    iκb kinase ikk β - by Bioz Stars, 2020-09
    92/100 stars

    Images

    1) Product Images from "Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma"

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2018.8945

    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P
    Figure Legend Snippet: Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Techniques Used: Activity Assay, Expressing, Migration

    2) Product Images from "Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma"

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2018.8945

    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P
    Figure Legend Snippet: Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Techniques Used: Activity Assay, Expressing, Migration

    Related Articles

    Western Blot:

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma
    Article Snippet: .. For western blotting, primary mouse anti-human antibodies against p65 (ab16502; 1:2,000), PI3K and (ab40776; 1:2,000), AKT (ab8805; 1:1,000), tumor necrosis factor (TNF)-α (ab6671; 1:2,000), IL-2 (cat. no. ab92381; 1:2,000), IκB kinase (IKK)-β (ab7547; 1:2,000), NF-κB inhibitor α (IκBα; ab133478; 1:2,000), pAKT (ab38449; 1:12,000) and β-actin (ab8827; 1:2,000; all from Abcam, Cambridge, UK) were added to the membranes after blocking with 5% skimmed milk for 2 h at 37°C. .. Following washing three times with PBS, membranes were incubated with secondary rabbit anti-mouse antibodies (PV-6001; 1:2,000; OriGene Technologies, Inc., Beijing, China) for 2 h at 37°C, in order to detect target proteins.

    Blocking Assay:

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma
    Article Snippet: .. For western blotting, primary mouse anti-human antibodies against p65 (ab16502; 1:2,000), PI3K and (ab40776; 1:2,000), AKT (ab8805; 1:1,000), tumor necrosis factor (TNF)-α (ab6671; 1:2,000), IL-2 (cat. no. ab92381; 1:2,000), IκB kinase (IKK)-β (ab7547; 1:2,000), NF-κB inhibitor α (IκBα; ab133478; 1:2,000), pAKT (ab38449; 1:12,000) and β-actin (ab8827; 1:2,000; all from Abcam, Cambridge, UK) were added to the membranes after blocking with 5% skimmed milk for 2 h at 37°C. .. Following washing three times with PBS, membranes were incubated with secondary rabbit anti-mouse antibodies (PV-6001; 1:2,000; OriGene Technologies, Inc., Beijing, China) for 2 h at 37°C, in order to detect target proteins.

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  • 92
    Abcam iκb kinase ikk β
    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, <t>IKK-β</t> and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P
    Iκb Kinase Ikk β, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iκb kinase ikk β/product/Abcam
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    iκb kinase ikk β - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    95
    Abcam nf κb iκb kinase kinase ikk β
    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; <t>IKK,</t> <t>IκB</t> kinase β; J, control; co, co-cultured.
    Nf κb Iκb Kinase Kinase Ikk β, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nf κb iκb kinase kinase ikk β/product/Abcam
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nf κb iκb kinase kinase ikk β - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Journal: Molecular Medicine Reports

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma

    doi: 10.3892/mmr.2018.8945

    Figure Lengend Snippet: Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Article Snippet: For western blotting, primary mouse anti-human antibodies against p65 (ab16502; 1:2,000), PI3K and (ab40776; 1:2,000), AKT (ab8805; 1:1,000), tumor necrosis factor (TNF)-α (ab6671; 1:2,000), IL-2 (cat. no. ab92381; 1:2,000), IκB kinase (IKK)-β (ab7547; 1:2,000), NF-κB inhibitor α (IκBα; ab133478; 1:2,000), pAKT (ab38449; 1:12,000) and β-actin (ab8827; 1:2,000; all from Abcam, Cambridge, UK) were added to the membranes after blocking with 5% skimmed milk for 2 h at 37°C.

    Techniques: Activity Assay, Expressing, Migration

    Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Journal: Molecular Medicine Reports

    Article Title: Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma

    doi: 10.3892/mmr.2018.8945

    Figure Lengend Snippet: Isoflurane regulates aggressiveness via the PI3K/protein kinase B-mediated NF-κB signaling pathway. (A) NF-κB activity in isoflurane-pretreated hepatic carcinoma cells. (B) Expression levels of p65, IKK-β and IκBα in isoflurane-pretreated hepatic carcinoma cells. (C) Effects of PI3KIR on NF-κB activity in hepatic carcinoma cells. (D) Effects of PI3KIR on migration and invasion of hepatic carcinoma cells. Control, non-treated cells. Data are presented as the mean ± standard error of the mean of three independent experiments. **P

    Article Snippet: For western blotting, primary mouse anti-human antibodies against p65 (ab16502; 1:2,000), PI3K and (ab40776; 1:2,000), AKT (ab8805; 1:1,000), tumor necrosis factor (TNF)-α (ab6671; 1:2,000), IL-2 (cat. no. ab92381; 1:2,000), IκB kinase (IKK)-β (ab7547; 1:2,000), NF-κB inhibitor α (IκBα; ab133478; 1:2,000), pAKT (ab38449; 1:12,000) and β-actin (ab8827; 1:2,000; all from Abcam, Cambridge, UK) were added to the membranes after blocking with 5% skimmed milk for 2 h at 37°C.

    Techniques: Activity Assay, Expressing, Migration

    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; IKK, IκB kinase β; J, control; co, co-cultured.

    Journal: International Journal of Molecular Medicine

    Article Title: Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK

    doi: 10.3892/ijmm.2018.3953

    Figure Lengend Snippet: Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; IKK, IκB kinase β; J, control; co, co-cultured.

    Article Snippet: The membranes were probed with primary antibodies against phosphorylated (p)-P65 (1:2,000, cat. no. AF2006, Affinity Biosciences, Cambridge, UK), P65 (1:2,000, cat. no. 10745-1-AP, ProteinTech Group, Inc., Wuhan, China), inhibitor of NF-κB (IκB) kinase (IKK)β (1:1,000, cat. no. Ab124957, Abcam, Cambridge, MA, USA), IκBα (1:1,000, cat. no. 4812, Cell Signaling Technology, Inc., Danvers, MA, USA), B-cell lymphoma 2 (Bcl-2; 1:2,000, cat. no. 12789-1-AP, ProteinTech Group, Inc.), Bcl-2-associated X protein (Bax; 1:5,000, cat. no. 50599-2-IG, Protein Tech Group, Inc), JNK1/2 (1:1,000, cat. no. 9252, Cell Signaling Technology, Inc.), p-JNK1/2 (1:1,000, cat. no. 4668, Cell Signaling Technology, Inc.), extracellular signal-regulated kinase (ERK)1/2 (1:1,000, cat. no. 9102, Cell Signaling Technology, Inc.), p-ERK1/2 (1:2,000, cat. no. 4370, Cell Signaling Technology, Inc.), P38 (1:1,000, cat. no. 9212, Cell Signaling, Technology, Inc.), p-P38 (1:1,000, cat. no. 9211, Cell Signaling Technology, Inc.), mothers against decapentaplegic (Smad)2/3 (1:1,000, cat. no. AF6367, Affinity Biosciences), p-Smad2/3 (1:200, cat. no. MAB8935, R & D Systems, Inc.) and the loading control β-actin (1:200, cat. no. BM0627, Boster Biological Technology, Wuhan, China).

    Techniques: Cell Culture, Western Blot, Expressing