Structured Review

Cell Signaling Technology Inc iκb kinase ikk α β
Iκb Kinase Ikk α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iκb kinase ikk α β/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
iκb kinase ikk α β - by Bioz Stars, 2020-09
90/100 stars

Images

Related Articles

other:

Article Title: Black ginseng-enriched Chong-Myung-Tang extracts improve spatial learning behavior in rats and elicit anti-inflammatory effects in vitro
Article Snippet: Specific antibodies used against phosphorylated extracellular signal-regulated kinase (ERK) and/or total form of ERK, Jun amino-terminal kinases (JNK), p38, IκB kinase (IKK) α/β, IκB, NF-κB p65, iNOS, COX-2, β-actin, and secondary antibody rabbit horseradish peroxidase-linked antibody were purchased from Cell Signaling Technology (Beverly, MA, USA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Cell Signaling Technology Inc iκb kinase p ikk α β
    Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor κB (NF-κB) activation in MCF-7 cells. (A) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. Translocation of p65 and p50 into the nucleus and phosphorylation of <t>IκB</t> kinase (IKK) <t>α/β</t> and nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (IκB) α in cytosol was determined by western blots. Proliferating cell nuclear antigen (PCNA) was the loading control for nuclear proteins. (B) NF-κB-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and NF-κB promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts made after 3 hours. NF-κB DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p
    Iκb Kinase P Ikk α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iκb kinase p ikk α β/product/Cell Signaling Technology Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    iκb kinase p ikk α β - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc phosphorylated iκb kinase ikk α β
    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
    Phosphorylated Iκb Kinase Ikk α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated iκb kinase ikk α β/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated iκb kinase ikk α β - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    88
    Cell Signaling Technology Inc iκb kinase
    Model of proposed signaling pathway. In mature osteoclasts, TGF-β rapidly activates both SMAD and <t>TAK1.</t> TAK1 activation leads to sequential activation of MEK1/2, AKT, NIK, and IKKα/β, leading to phosphorylation of <t>IκB.</t> This causes targeted degradation of IκB, allowing subsequent NFκB nuclear localization to promote osteoclast survival. TGF-β-dependent SMAD2/3 activation and nuclear localization also occurs in parallel. NFκB activates transcription of BCLX L and Mcl-1 whereas SMAD2/3 activate transcription of Mcl-1.
    Iκb Kinase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iκb kinase/product/Cell Signaling Technology Inc
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    iκb kinase - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor κB (NF-κB) activation in MCF-7 cells. (A) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. Translocation of p65 and p50 into the nucleus and phosphorylation of IκB kinase (IKK) α/β and nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (IκB) α in cytosol was determined by western blots. Proliferating cell nuclear antigen (PCNA) was the loading control for nuclear proteins. (B) NF-κB-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and NF-κB promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts made after 3 hours. NF-κB DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Journal: Journal of Breast Cancer

    Article Title: Troglitazone Inhibits Matrix Metalloproteinase-9 Expression and Invasion of Breast Cancer Cell through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism

    doi: 10.4048/jbc.2018.21.1.28

    Figure Lengend Snippet: Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor κB (NF-κB) activation in MCF-7 cells. (A) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. Translocation of p65 and p50 into the nucleus and phosphorylation of IκB kinase (IKK) α/β and nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (IκB) α in cytosol was determined by western blots. Proliferating cell nuclear antigen (PCNA) was the loading control for nuclear proteins. (B) NF-κB-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and NF-κB promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts made after 3 hours. NF-κB DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Article Snippet: Phosphorylated (p)-c-Jun, nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (p-IκB) α, IκB kinase (p-IKK) α/β, c-Jun N-terminal kinases (JNK), p-JNK, extracellular signal-regulated kinases (ERK), p-ERK, and p-protein kinase B (AKT) antibodies were purchased from Cell Signaling Technology (Danvers, USA).

    Techniques: Activation Assay, Translocation Assay, Western Blot, Luciferase, Plasmid Preparation, Transfection, Activity Assay, Binding Assay, Electrophoretic Mobility Shift Assay

    Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor κB (NF-κB) activation in MCF-7 cells. (A) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. Translocation of p65 and p50 into the nucleus and phosphorylation of IκB kinase (IKK) α/β and nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (IκB) α in cytosol was determined by western blots. Proliferating cell nuclear antigen (PCNA) was the loading control for nuclear proteins. (B) NF-κB-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and NF-κB promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts made after 3 hours. NF-κB DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Journal: Journal of Breast Cancer

    Article Title: Troglitazone Inhibits Matrix Metalloproteinase-9 Expression and Invasion of Breast Cancer Cell through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism

    doi: 10.4048/jbc.2018.21.1.28

    Figure Lengend Snippet: Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor κB (NF-κB) activation in MCF-7 cells. (A) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. Translocation of p65 and p50 into the nucleus and phosphorylation of IκB kinase (IKK) α/β and nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (IκB) α in cytosol was determined by western blots. Proliferating cell nuclear antigen (PCNA) was the loading control for nuclear proteins. (B) NF-κB-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and NF-κB promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts made after 3 hours. NF-κB DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Article Snippet: Phosphorylated (p)-c-Jun, nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (p-IκB) α, IκB kinase (p-IKK) α/β, c-Jun N-terminal kinases (JNK), p-JNK, extracellular signal-regulated kinases (ERK), p-ERK, and p-protein kinase B (AKT) antibodies were purchased from Cell Signaling Technology (Danvers, USA).

    Techniques: Activation Assay, Translocation Assay, Western Blot, Luciferase, Plasmid Preparation, Transfection, Activity Assay, Binding Assay, Electrophoretic Mobility Shift Assay

    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p

    Journal: Nutrients

    Article Title: Immune-Enhancing Effects of a High Molecular Weight Fraction of Cynanchum wilfordii Hemsley in Macrophages and Immunosuppressed Mice

    doi: 10.3390/nu8100600

    Figure Lengend Snippet: HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p

    Article Snippet: Antibodies against phosphorylated-inhibitor of kappa B (IκB)-α, phosphorylated-IκB kinase (IKK)-α/β, and phosphorylated-p38 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

    Techniques: Activation Assay, Isolation

    Model of proposed signaling pathway. In mature osteoclasts, TGF-β rapidly activates both SMAD and TAK1. TAK1 activation leads to sequential activation of MEK1/2, AKT, NIK, and IKKα/β, leading to phosphorylation of IκB. This causes targeted degradation of IκB, allowing subsequent NFκB nuclear localization to promote osteoclast survival. TGF-β-dependent SMAD2/3 activation and nuclear localization also occurs in parallel. NFκB activates transcription of BCLX L and Mcl-1 whereas SMAD2/3 activate transcription of Mcl-1.

    Journal: Experimental cell research

    Article Title: TGF-? Coordinately Activates TAK1/MEK/AKT/NFkB and Smad Pathways to Promote Osteoclast Survival

    doi: 10.1016/j.yexcr.2008.06.006

    Figure Lengend Snippet: Model of proposed signaling pathway. In mature osteoclasts, TGF-β rapidly activates both SMAD and TAK1. TAK1 activation leads to sequential activation of MEK1/2, AKT, NIK, and IKKα/β, leading to phosphorylation of IκB. This causes targeted degradation of IκB, allowing subsequent NFκB nuclear localization to promote osteoclast survival. TGF-β-dependent SMAD2/3 activation and nuclear localization also occurs in parallel. NFκB activates transcription of BCLX L and Mcl-1 whereas SMAD2/3 activate transcription of Mcl-1.

    Article Snippet: Anti phospho- and total antibodies directed toward Tak1 (Thr184/187), MEK 1/2 (Ser217/221), AKT (Ser473), Inhibitor of IκB Kinase (IKK)α/β (Ser176/180), Inhibitor of NFκB (IκB) (Ser32), and NFκB (Ser536) (Cell Signaling, Beverly, MA) were used at a 1:1,000 dilution for Western blotting according to the product directions as we have reported [ ].

    Techniques: Activation Assay