hyperoxia chamber (BioSpherix)

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BioSpherix hyperoxia chamber

a, Heatmap of Pearson correlations calculated between the HPCS and recurrent pan-cancer cell states (AC: astrocyte-like cell state, OPC: oligodendrocyte progenitor cell-like, NPC: neural progenitor cell-like) , divided by cancer type (BC: breast cancer, CRC: colorectal cancer, HNC: head and neck cancer, LUAD: lung adenocarcinoma, OC: ovarian cancer, PCa: prostate adenocarcinoma, PDAC: pancreatic ductal adenocarcinoma, SCC: squamous cell carcinoma of the skin, ALL: acute lymphoblastic leukemia, MM: multiple myeloma). Arrow indicates the Stress -associated cell state from ref. . A full list of studies is listed in Supplementary Table 5 . b, Pearson correlation between the HPCS signature score ( x -axis) and plastic cell states ( y -axis) from either colorectal cancer (OF/HRC: oncofetal/core high relapse , ) ( top ), and pancreatic ductal adenocarcinoma (scBasal ) ( middle ), or castration-resistant prostate cancer adenocarcinoma (Plasticity ) ( bottom ). Two-tailed p value calculated from the exact distribution of Pearson correlation coefficient. c , Heatmaps demonstrating mean gene signature score in either SLC4A11 or PLAUR negative and positive cells scaled using a z-score generated per program to compare gene signatures across different cancer types in human scRNA-seq datasets ( Supplementary Table 5 ). For each dataset gene pair, significance is calculated by comparing positive vs negative cells and depicted in the positive cell column. Four human LUAD datasets are shown [LungM 5 ; LungK ; LungB ; LungD ] along with plastic cell states [OF/HRC , , lowerSpike , Plasticity ] in other cancers [CRC ; SCC ; PCa ; PDAC ; HNC_C , HNC_K , OC ]. t-test. d, Immunofluorescence of human LUAD stained for (top) uPAR (green) and integrin α2 (red) or (bottom) pan-cytokeratin (white). Arrows point to uPAR and integrin α2 double positive cells. Scale bar: 25 μm. e, Heatmaps demonstrating mean gene signature score in either Slc4a11 or Plaur negative and positive cells scaled using a z-score generated per program to compare gene signatures across different injury models in mouse single cell datasets ( Supplementary Table 5 ). Heatmaps of signature scores represented as z-scores of the indicated regenerative programs in Lung (DATP: damage associated transient progenitor ; ADI: KRT8 + alveolar differentiation intermediate ; PATS: pre-alveolar type-1 transitional cell state ), Pancreas (scBasal cell state ), Intestine (revSC: revival stem cell ; SSC2: singly profiled stem cell cluster 2 ) and Skin (Wound ). For each dataset gene pair, significance is calculated by comparing positive vs negative cells and depicted in the positive cell column. t-test. f, Representative multiplex FISH staining of Slc4a11 (red) and Sftpc (green) in normal lungs vs. 7 days following <t>hyperoxia-induced</t> lung injury. Scale bar: 10 µm.

Hyperoxia Chamber, supplied by BioSpherix, used in various techniques. Bioz Stars score: 96/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hyperoxia+chamber/C-Chamber/bio_rxiv__2025__10__03__680232-357-19-21
Average 96 stars, based on 432 article reviews

hyperoxia chamber - by Bioz Stars, 2026-10

96/100 stars

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1) Product Images from "Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma"

Article Title: Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma

Journal: bioRxiv

doi: 10.1101/2025.10.03.680232

Figure Legend Snippet: a, Heatmap of Pearson correlations calculated between the HPCS and recurrent pan-cancer cell states (AC: astrocyte-like cell state, OPC: oligodendrocyte progenitor cell-like, NPC: neural progenitor cell-like) , divided by cancer type (BC: breast cancer, CRC: colorectal cancer, HNC: head and neck cancer, LUAD: lung adenocarcinoma, OC: ovarian cancer, PCa: prostate adenocarcinoma, PDAC: pancreatic ductal adenocarcinoma, SCC: squamous cell carcinoma of the skin, ALL: acute lymphoblastic leukemia, MM: multiple myeloma). Arrow indicates the Stress -associated cell state from ref. . A full list of studies is listed in Supplementary Table 5 . b, Pearson correlation between the HPCS signature score ( x -axis) and plastic cell states ( y -axis) from either colorectal cancer (OF/HRC: oncofetal/core high relapse , ) ( top ), and pancreatic ductal adenocarcinoma (scBasal ) ( middle ), or castration-resistant prostate cancer adenocarcinoma (Plasticity ) ( bottom ). Two-tailed p value calculated from the exact distribution of Pearson correlation coefficient. c , Heatmaps demonstrating mean gene signature score in either SLC4A11 or PLAUR negative and positive cells scaled using a z-score generated per program to compare gene signatures across different cancer types in human scRNA-seq datasets ( Supplementary Table 5 ). For each dataset gene pair, significance is calculated by comparing positive vs negative cells and depicted in the positive cell column. Four human LUAD datasets are shown [LungM 5 ; LungK ; LungB ; LungD ] along with plastic cell states [OF/HRC , , lowerSpike , Plasticity ] in other cancers [CRC ; SCC ; PCa ; PDAC ; HNC_C , HNC_K , OC ]. t-test. d, Immunofluorescence of human LUAD stained for (top) uPAR (green) and integrin α2 (red) or (bottom) pan-cytokeratin (white). Arrows point to uPAR and integrin α2 double positive cells. Scale bar: 25 μm. e, Heatmaps demonstrating mean gene signature score in either Slc4a11 or Plaur negative and positive cells scaled using a z-score generated per program to compare gene signatures across different injury models in mouse single cell datasets ( Supplementary Table 5 ). Heatmaps of signature scores represented as z-scores of the indicated regenerative programs in Lung (DATP: damage associated transient progenitor ; ADI: KRT8 + alveolar differentiation intermediate ; PATS: pre-alveolar type-1 transitional cell state ), Pancreas (scBasal cell state ), Intestine (revSC: revival stem cell ; SSC2: singly profiled stem cell cluster 2 ) and Skin (Wound ). For each dataset gene pair, significance is calculated by comparing positive vs negative cells and depicted in the positive cell column. t-test. f, Representative multiplex FISH staining of Slc4a11 (red) and Sftpc (green) in normal lungs vs. 7 days following hyperoxia-induced lung injury. Scale bar: 10 µm.

Techniques Used: Two Tailed Test, Generated, Immunofluorescence, Staining, Multiplex Assay

Construct:

Article Title: Single-Cell Multiomics Profiling Reveals Heterogeneity of Müller Cells in the Oxygen-Induced Retinopathy Model.
Article Snippet: The animal experiments involving mice and the OIR model were reviewed and approved by the ethics committee of Tianjin Medical University General Hospital (TMUaMEC 2020006). .. To construct the mouse OIR model, postnatal day 7 (P7) pups along with their nursing mothers were exposed to a hyperoxia chamber regulated by a calibrated controller and an oxygen sensor (BioSpherix, Parish, NY), where they were exposed to 75% O2 until P12. ..

Article Title: Single-Cell Multiomics Profiling Reveals Heterogeneity of Müller Cells in the Oxygen-Induced Retinopathy Model
Article Snippet: The animal experiments involving mice and the OIR model were reviewed and approved by the ethics committee of Tianjin Medical University General Hospital (TMUaMEC 2020006). .. To construct the mouse OIR model, postnatal day 7 (P7) pups along with their nursing mothers were exposed to a hyperoxia chamber regulated by a calibrated controller and an oxygen sensor (BioSpherix, Parish, NY), where they were exposed to 75% O 2 until P12. ..

Concentration Assay:

Article Title: Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma
Article Snippet: Purified T cells were cultured in RPMI-1640 (Fisher Scientific, #11-875-119) supplemented with 10% FBS (GeminiBio, #900-108), 10 mM HEPES (Fisher Scientific, #15-630-080), 2 mM l-glutamine (Thermo Scientific, #A2916801), MEM non-essential amino acids 1x (Thermo Fisher Scientific, #11140050), 55 μM β-mercaptoethanol (Thermo Scientific, #A2916801), 1 mM sodium pyruvate (Thermo Scientific, #11360070), 100 IU ml−1 recombinant human IL-2 (Proleukin; Novartis) and mouse anti-CD3/28 Dynabeads (Gibco, #11453D) at a bead:cell ratio of 1:2. .. Alveolar injury was induced in young C57BL/6 mice by a 32 hour exposure to >85% O 2 in a hyperoxia chamber (BioSpherix), with FiO 2 concentration maintained at a constant flow of ∼3 L O 2 /minute and monitored by an in-line oxygen analyzer. ..

other:

Article Title: Targeted degradation of VEGF with bispecific aptamer-based LYTACs ameliorates pathological retinal angiogenesis.
Article Snippet: In brief, neonatal mice at P7 were placed in a controlled hyperoxia chamber (BioSpherix, Parish, NY), where they were exposed to a consistently maintained atmosphere containing 75% oxygen for a duration of 5 d. Subsequent to this hyperoxic exposure, the mice were returned to normoxic conditions.

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