hygromycin resistance genes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hygromycin resistance genes
    Hygromycin Resistance Genes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hygromycin resistance genes/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hygromycin resistance genes - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Signal transducer and activator of transcription 3 activation up-regulates interleukin-6 autocrine production: a biochemical and genetic study of established cancer cell lines and clinical isolated human cancer cells
    Article Snippet: .. After transfection, we treated the cells with Hygromycin-B (Invitrogen) for more than 3 weeks to select stable cell lines containing Stat3 shRNA or control plasmid. .. The stable cell lines were maintained in media containing 300 μM Hygromycin-B and passaged once in the absence of Hygromycin-B before treatment.

    Selection:

    Article Title: Phosphorylation State of Ser165 in α-Tubulin is a Toggle Switch That Controls Proliferating Human Breast Tumors
    Article Snippet: .. Stable transfectants were isolated under selection with 500 ng/ml hygromycin (Life Technologies). .. Resulting clones were maintained at a hygromycin concentration of 200 ng/ml, and G418 (300 ng/ml) (Sigma-Aldrich).

    Stable Transfection:

    Article Title: Signal transducer and activator of transcription 3 activation up-regulates interleukin-6 autocrine production: a biochemical and genetic study of established cancer cell lines and clinical isolated human cancer cells
    Article Snippet: .. After transfection, we treated the cells with Hygromycin-B (Invitrogen) for more than 3 weeks to select stable cell lines containing Stat3 shRNA or control plasmid. .. The stable cell lines were maintained in media containing 300 μM Hygromycin-B and passaged once in the absence of Hygromycin-B before treatment.

    Isolation:

    Article Title: Phosphorylation State of Ser165 in α-Tubulin is a Toggle Switch That Controls Proliferating Human Breast Tumors
    Article Snippet: .. Stable transfectants were isolated under selection with 500 ng/ml hygromycin (Life Technologies). .. Resulting clones were maintained at a hygromycin concentration of 200 ng/ml, and G418 (300 ng/ml) (Sigma-Aldrich).

    Concentration Assay:

    Article Title: Conditional gene inactivation by combining tetracycline-mediated transcriptional repression and auxin-inducible degron-mediated degradation
    Article Snippet: .. Cells were treated with the following reagents at the indicated final concentration: blasticidin (5 µg/ml) (Life Technologies, Carlsbad, CA, USA), doxycycline hydrochloride (Dox) (2 µg/ml), hygromycin B (200 µg/ml) (Life Technologies), indole-3-acetic acid (IAA) (50 µg/ml) (Sigma-Aldrich), nocodazole (0.33 µM) (Enzo Life Sciences, Farmingdale, NY, USA), and puromycin (0.6 µg/ml) (Sigma-Aldrich). .. AID-cyclin A in cyclin AKO cells were generated by first infecting HeLa cells with retroviruses expressing AID-cyclin A and grown in medium containing hygromycin B for 2 wk.

    shRNA:

    Article Title: Signal transducer and activator of transcription 3 activation up-regulates interleukin-6 autocrine production: a biochemical and genetic study of established cancer cell lines and clinical isolated human cancer cells
    Article Snippet: .. After transfection, we treated the cells with Hygromycin-B (Invitrogen) for more than 3 weeks to select stable cell lines containing Stat3 shRNA or control plasmid. .. The stable cell lines were maintained in media containing 300 μM Hygromycin-B and passaged once in the absence of Hygromycin-B before treatment.

    Modification:

    Article Title: Viroporin activity of the JC polyomavirus is regulated by interactions with the adaptor protein complex 3
    Article Snippet: .. Permeability of the plasma membranes to Hygromycin B (HygB) was determined using the Click-iT l -azidohomoalanine (AHA) Alexa Fluor 488 Protein Synthesis HCS Assay kit (Invitrogen) with slight modification. ..

    Transformation Assay:

    Article Title: Rapid, direct detection of bacterial Topoisomerase 1-DNA adducts by RADAR/ELISA
    Article Snippet: .. M. smegmatis was transformed using a MicroPulser™ Electroporator Bio-Rad), and transformed cells selected and propagated in medium containing 200 μg/ml hygromycin B (Thermo-Fisher). .. MtTop1 expression was induced in exponentially growing (OD600 =0.5) cultures of M. smegmatis by addition of 50 ng/ml anhydrotetracycline (ATc; Takara-Clontech) ( ).

    Permeability:

    Article Title: Viroporin activity of the JC polyomavirus is regulated by interactions with the adaptor protein complex 3
    Article Snippet: .. Permeability of the plasma membranes to Hygromycin B (HygB) was determined using the Click-iT l -azidohomoalanine (AHA) Alexa Fluor 488 Protein Synthesis HCS Assay kit (Invitrogen) with slight modification. ..

    Plasmid Preparation:

    Article Title: Signal transducer and activator of transcription 3 activation up-regulates interleukin-6 autocrine production: a biochemical and genetic study of established cancer cell lines and clinical isolated human cancer cells
    Article Snippet: .. After transfection, we treated the cells with Hygromycin-B (Invitrogen) for more than 3 weeks to select stable cell lines containing Stat3 shRNA or control plasmid. .. The stable cell lines were maintained in media containing 300 μM Hygromycin-B and passaged once in the absence of Hygromycin-B before treatment.

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    Thermo Fisher hygromycin resistance gene
    Modeling homologous recombination through Chi AD . (A) Constructs for in vitro recombination were generated on the <t>pcDNA3.1-hygromycin/neomycin</t> by insertion of either the penton base gene of HAdV-D64 while retaining the hygromycin resistance gene or of HAdV-D22 with coding sequence for green fluorescent protein (GFP) without ATG site, no CMVT7 promoter, and retaining the neomycin resistance gene. HVL2 (yellow boxes), GC/AT transition zone (red boxes with blue arrowhead above), CMVT7 promoter (gray boxes), and GFP open reading frames (green boxes) are indicated. EVH and EVN are hygromycin and neomycin empty vectors minus their CMVT7 promoters, respectively. Modified vectors were linearized with NruI prior to transfection. (B) Fluorescence microscopy for GFP expression in transfected 293A cells show green signal indicating recombination only when both HAdV-D sequence constructs are cotransfected (right micrograph). Original magnification, ×40. Bar, 25 µm. (C) Fluorescent signal graphically represented relative to GFP with cotransfection of an empty vector, demonstrating approximately threefold increase in signal upon cotransfection of Chi AD -containing constructs. The value that is significantly different ( P
    Hygromycin Resistance Gene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hygromycin resistance gene/product/Thermo Fisher
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    hygromycin resistance gene - by Bioz Stars, 2020-09
    91/100 stars
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    Modeling homologous recombination through Chi AD . (A) Constructs for in vitro recombination were generated on the pcDNA3.1-hygromycin/neomycin by insertion of either the penton base gene of HAdV-D64 while retaining the hygromycin resistance gene or of HAdV-D22 with coding sequence for green fluorescent protein (GFP) without ATG site, no CMVT7 promoter, and retaining the neomycin resistance gene. HVL2 (yellow boxes), GC/AT transition zone (red boxes with blue arrowhead above), CMVT7 promoter (gray boxes), and GFP open reading frames (green boxes) are indicated. EVH and EVN are hygromycin and neomycin empty vectors minus their CMVT7 promoters, respectively. Modified vectors were linearized with NruI prior to transfection. (B) Fluorescence microscopy for GFP expression in transfected 293A cells show green signal indicating recombination only when both HAdV-D sequence constructs are cotransfected (right micrograph). Original magnification, ×40. Bar, 25 µm. (C) Fluorescent signal graphically represented relative to GFP with cotransfection of an empty vector, demonstrating approximately threefold increase in signal upon cotransfection of Chi AD -containing constructs. The value that is significantly different ( P

    Journal: mSphere

    Article Title: Bacterial RecA Protein Promotes Adenoviral Recombination during In Vitro Infection

    doi: 10.1128/mSphere.00105-18

    Figure Lengend Snippet: Modeling homologous recombination through Chi AD . (A) Constructs for in vitro recombination were generated on the pcDNA3.1-hygromycin/neomycin by insertion of either the penton base gene of HAdV-D64 while retaining the hygromycin resistance gene or of HAdV-D22 with coding sequence for green fluorescent protein (GFP) without ATG site, no CMVT7 promoter, and retaining the neomycin resistance gene. HVL2 (yellow boxes), GC/AT transition zone (red boxes with blue arrowhead above), CMVT7 promoter (gray boxes), and GFP open reading frames (green boxes) are indicated. EVH and EVN are hygromycin and neomycin empty vectors minus their CMVT7 promoters, respectively. Modified vectors were linearized with NruI prior to transfection. (B) Fluorescence microscopy for GFP expression in transfected 293A cells show green signal indicating recombination only when both HAdV-D sequence constructs are cotransfected (right micrograph). Original magnification, ×40. Bar, 25 µm. (C) Fluorescent signal graphically represented relative to GFP with cotransfection of an empty vector, demonstrating approximately threefold increase in signal upon cotransfection of Chi AD -containing constructs. The value that is significantly different ( P

    Article Snippet: The pcDNA3.1-hygromycin vector was obtained from Thermo Fisher Scientific, and the hygromycin resistance gene replaced with one for neomycin from pcDNA3.1-myc-his-A(+) (Thermo Fisher Scientific).

    Techniques: Homologous Recombination, Construct, In Vitro, Generated, Sequencing, Modification, Transfection, Fluorescence, Microscopy, Expressing, Cotransfection, Plasmid Preparation

    Modeling homologous recombination through Chi AD . (A) Constructs for in vitro recombination were generated on the pcDNA3.1-hygromycin/neomycin by insertion of either the penton base gene of HAdV-D64 while retaining the hygromycin resistance gene or of HAdV-D22 with coding sequence for green fluorescent protein (GFP) without ATG site, no CMVT7 promoter, and retaining the neomycin resistance gene. HVL2 (yellow boxes), GC/AT transition zone (red boxes with blue arrowhead above), CMVT7 promoter (gray boxes), and GFP open reading frames (green boxes) are indicated. EVH and EVN are hygromycin and neomycin empty vectors minus their CMVT7 promoters, respectively. Modified vectors were linearized with NruI prior to transfection. (B) Fluorescence microscopy for GFP expression in transfected 293A cells show green signal indicating recombination only when both HAdV-D sequence constructs are cotransfected (right micrograph). Original magnification, ×40. Bar, 25 µm. (C) Fluorescent signal graphically represented relative to GFP with cotransfection of an empty vector, demonstrating approximately threefold increase in signal upon cotransfection of Chi AD -containing constructs. The value that is significantly different ( P

    Journal: mSphere

    Article Title: Bacterial RecA Protein Promotes Adenoviral Recombination during In Vitro Infection

    doi: 10.1128/mSphere.00105-18

    Figure Lengend Snippet: Modeling homologous recombination through Chi AD . (A) Constructs for in vitro recombination were generated on the pcDNA3.1-hygromycin/neomycin by insertion of either the penton base gene of HAdV-D64 while retaining the hygromycin resistance gene or of HAdV-D22 with coding sequence for green fluorescent protein (GFP) without ATG site, no CMVT7 promoter, and retaining the neomycin resistance gene. HVL2 (yellow boxes), GC/AT transition zone (red boxes with blue arrowhead above), CMVT7 promoter (gray boxes), and GFP open reading frames (green boxes) are indicated. EVH and EVN are hygromycin and neomycin empty vectors minus their CMVT7 promoters, respectively. Modified vectors were linearized with NruI prior to transfection. (B) Fluorescence microscopy for GFP expression in transfected 293A cells show green signal indicating recombination only when both HAdV-D sequence constructs are cotransfected (right micrograph). Original magnification, ×40. Bar, 25 µm. (C) Fluorescent signal graphically represented relative to GFP with cotransfection of an empty vector, demonstrating approximately threefold increase in signal upon cotransfection of Chi AD -containing constructs. The value that is significantly different ( P

    Article Snippet: The pcDNA3.1-hygromycin vector was obtained from Thermo Fisher Scientific, and the hygromycin resistance gene replaced with one for neomycin from pcDNA3.1-myc-his-A(+) (Thermo Fisher Scientific).

    Techniques: Homologous Recombination, Construct, In Vitro, Generated, Sequencing, Modification, Transfection, Fluorescence, Microscopy, Expressing, Cotransfection, Plasmid Preparation